LOCUS       DACIWG010000001        81218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.001, whole genome shotgun sequence.
ACCESSION   DACIWG010000001 DACIWG010000000
VERSION     DACIWG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.001"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            102..1373
                     /locus_tag="IYF54_000001"
     CDS             102..1373
                     /locus_tag="IYF54_000001"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ9830375.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            1370..2665
                     /locus_tag="IYF54_000002"
     CDS             1370..2665
                     /locus_tag="IYF54_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ9830376.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            2764..3027
                     /locus_tag="IYF54_000003"
     CDS             2764..3027
                     /locus_tag="IYF54_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ9830377.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            3472..3921
                     /locus_tag="IYF54_000004"
     CDS             3472..3921
                     /locus_tag="IYF54_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9830378.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            3978..5396
                     /locus_tag="IYF54_000005"
     CDS             3978..5396
                     /locus_tag="IYF54_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830379.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            5396..6256
                     /locus_tag="IYF54_000006"
     CDS             5396..6256
                     /locus_tag="IYF54_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ9830380.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            6430..7203
                     /locus_tag="IYF54_000007"
     CDS             6430..7203
                     /locus_tag="IYF54_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9830381.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            7350..7715
                     /locus_tag="IYF54_000008"
     CDS             7350..7715
                     /locus_tag="IYF54_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ9830382.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(7863..9071)
                     /locus_tag="IYF54_000009"
     CDS             complement(7863..9071)
                     /locus_tag="IYF54_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9830383.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(9090..9368)
                     /locus_tag="IYF54_000010"
     CDS             complement(9090..9368)
                     /locus_tag="IYF54_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830384.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            9561..10217
                     /locus_tag="IYF54_000011"
     CDS             9561..10217
                     /locus_tag="IYF54_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9830385.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(10659..11456)
                     /locus_tag="IYF54_000012"
     CDS             complement(10659..11456)
                     /locus_tag="IYF54_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ9830386.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(11453..12637)
                     /locus_tag="IYF54_000013"
     CDS             complement(11453..12637)
                     /locus_tag="IYF54_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ9830387.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(12662..13909)
                     /locus_tag="IYF54_000014"
     CDS             complement(12662..13909)
                     /locus_tag="IYF54_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ9830388.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            14124..15869
                     /gene="dnaX"
                     /locus_tag="IYF54_000015"
     CDS             14124..15869
                     /gene="dnaX"
                     /locus_tag="IYF54_000015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ9830389.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            15889..16203
                     /locus_tag="IYF54_000016"
     CDS             15889..16203
                     /locus_tag="IYF54_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ9830390.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            16228..16824
                     /gene="recR"
                     /locus_tag="IYF54_000017"
     CDS             16228..16824
                     /gene="recR"
                     /locus_tag="IYF54_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ9830391.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            16888..17532
                     /locus_tag="IYF54_000018"
     CDS             16888..17532
                     /locus_tag="IYF54_000018"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ9830392.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            17547..17876
                     /locus_tag="IYF54_000019"
     CDS             17547..17876
                     /locus_tag="IYF54_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830393.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            17890..18828
                     /gene="holB"
                     /locus_tag="IYF54_000020"
     CDS             17890..18828
                     /gene="holB"
                     /locus_tag="IYF54_000020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ9830394.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            18864..19688
                     /locus_tag="IYF54_000021"
     CDS             18864..19688
                     /locus_tag="IYF54_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ9830395.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            19681..20028
                     /locus_tag="IYF54_000022"
     CDS             19681..20028
                     /locus_tag="IYF54_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ9830396.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            20097..20969
                     /gene="rsmI"
                     /locus_tag="IYF54_000023"
     CDS             20097..20969
                     /gene="rsmI"
                     /locus_tag="IYF54_000023"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ9830397.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            21078..22199
                     /gene="dinB"
                     /locus_tag="IYF54_000024"
     CDS             21078..22199
                     /gene="dinB"
                     /locus_tag="IYF54_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ9830398.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(22253..22855)
                     /gene="nrdG"
                     /locus_tag="IYF54_000025"
     CDS             complement(22253..22855)
                     /gene="nrdG"
                     /locus_tag="IYF54_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ9830399.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(23170..25359)
                     /gene="nrdD"
                     /locus_tag="IYF54_000026"
     CDS             complement(23170..25359)
                     /gene="nrdD"
                     /locus_tag="IYF54_000026"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ9830400.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            25685..26452
                     /locus_tag="IYF54_000027"
     CDS             25685..26452
                     /locus_tag="IYF54_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830401.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            26442..28484
                     /locus_tag="IYF54_000028"
     CDS             26442..28484
                     /locus_tag="IYF54_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830402.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            28765..28914
                     /locus_tag="IYF54_000029"
     CDS             28765..28914
                     /locus_tag="IYF54_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ9830403.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            28925..30439
                     /gene="dltA"
                     /locus_tag="IYF54_000030"
     CDS             28925..30439
                     /gene="dltA"
                     /locus_tag="IYF54_000030"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ9830404.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            30436..31641
                     /gene="dltB"
                     /locus_tag="IYF54_000031"
     CDS             30436..31641
                     /gene="dltB"
                     /locus_tag="IYF54_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ9830405.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            31686..31919
                     /gene="dltC"
                     /locus_tag="IYF54_000032"
     CDS             31686..31919
                     /gene="dltC"
                     /locus_tag="IYF54_000032"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ9830406.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            31922..33190
                     /gene="dltD"
                     /locus_tag="IYF54_000033"
     CDS             31922..33190
                     /gene="dltD"
                     /locus_tag="IYF54_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ9830407.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            33331..34413
                     /locus_tag="IYF54_000034"
     CDS             33331..34413
                     /locus_tag="IYF54_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ9830408.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            34413..35216
                     /locus_tag="IYF54_000035"
     CDS             34413..35216
                     /locus_tag="IYF54_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ9830409.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            35286..36026
                     /locus_tag="IYF54_000036"
     CDS             35286..36026
                     /locus_tag="IYF54_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9830410.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            36037..37041
                     /locus_tag="IYF54_000037"
     CDS             36037..37041
                     /locus_tag="IYF54_000037"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ9830411.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(37086..38930)
                     /locus_tag="IYF54_000038"
     CDS             complement(37086..38930)
                     /locus_tag="IYF54_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830412.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            39142..39660
                     /locus_tag="IYF54_000039"
     CDS             39142..39660
                     /locus_tag="IYF54_000039"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9830413.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            39788..40471
                     /locus_tag="IYF54_000040"
     CDS             39788..40471
                     /locus_tag="IYF54_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ9830414.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            40780..40853
                     /locus_tag="IYF54_000041"
     tRNA            40780..40853
                     /locus_tag="IYF54_000041"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40814..40816,aa:Arg,seq:tct)
     gene            41012..41371
                     /locus_tag="IYF54_000042"
     CDS             41012..41371
                     /locus_tag="IYF54_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ9830415.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            41494..43125
                     /locus_tag="IYF54_000043"
     CDS             41494..43125
                     /locus_tag="IYF54_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ9830416.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(43187..43828)
                     /locus_tag="IYF54_000044"
     CDS             complement(43187..43828)
                     /locus_tag="IYF54_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ9830417.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            44110..44418
                     /gene="rplU"
                     /locus_tag="IYF54_000045"
     CDS             44110..44418
                     /gene="rplU"
                     /locus_tag="IYF54_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ9830418.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            44445..44789
                     /locus_tag="IYF54_000046"
     CDS             44445..44789
                     /locus_tag="IYF54_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ9830419.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            44802..45095
                     /gene="rpmA"
                     /locus_tag="IYF54_000047"
     CDS             44802..45095
                     /gene="rpmA"
                     /locus_tag="IYF54_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ9830420.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            45502..48336
                     /locus_tag="IYF54_000048"
     CDS             45502..48336
                     /locus_tag="IYF54_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9830421.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            48527..49018
                     /locus_tag="IYF54_000049"
     CDS             48527..49018
                     /locus_tag="IYF54_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ9830422.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            49121..50110
                     /gene="manX"
                     /locus_tag="IYF54_000050"
     CDS             49121..50110
                     /gene="manX"
                     /locus_tag="IYF54_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ9830423.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            50143..50946
                     /locus_tag="IYF54_000051"
     CDS             50143..50946
                     /locus_tag="IYF54_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ9830424.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            50967..51881
                     /locus_tag="IYF54_000052"
     CDS             50967..51881
                     /locus_tag="IYF54_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ9830425.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            51964..52338
                     /locus_tag="IYF54_000053"
     CDS             51964..52338
                     /locus_tag="IYF54_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ9830426.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            52477..52722
                     /locus_tag="IYF54_000054"
     CDS             52477..52722
                     /locus_tag="IYF54_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9830427.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            52894..53958
                     /locus_tag="IYF54_000055"
     CDS             52894..53958
                     /locus_tag="IYF54_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ9830428.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            54204..54629
                     /locus_tag="IYF54_000056"
     CDS             54204..54629
                     /locus_tag="IYF54_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9830429.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            54622..55071
                     /gene="nusB"
                     /locus_tag="IYF54_000057"
     CDS             54622..55071
                     /gene="nusB"
                     /locus_tag="IYF54_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ9830430.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            55168..56022
                     /locus_tag="IYF54_000058"
     CDS             55168..56022
                     /locus_tag="IYF54_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ9830431.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            56023..57360
                     /locus_tag="IYF54_000059"
     CDS             56023..57360
                     /locus_tag="IYF54_000059"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ9830432.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            57365..57589
                     /locus_tag="IYF54_000060"
     CDS             57365..57589
                     /locus_tag="IYF54_000060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ9830433.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            57591..58481
                     /locus_tag="IYF54_000061"
     CDS             57591..58481
                     /locus_tag="IYF54_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ9830434.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLTRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            58490..59308
                     /locus_tag="IYF54_000062"
     CDS             58490..59308
                     /locus_tag="IYF54_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ9830435.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            59415..59864
                     /locus_tag="IYF54_000063"
     CDS             59415..59864
                     /locus_tag="IYF54_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ9830436.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            59882..61564
                     /gene="recN"
                     /locus_tag="IYF54_000064"
     CDS             59882..61564
                     /gene="recN"
                     /locus_tag="IYF54_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ9830437.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(61619..61735)
                     /locus_tag="IYF54_000065"
     CDS             complement(61619..61735)
                     /locus_tag="IYF54_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ9830438.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(61808..62764)
                     /locus_tag="IYF54_000066"
     CDS             complement(61808..62764)
                     /locus_tag="IYF54_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9830439.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            62935..63303
                     /locus_tag="IYF54_000067"
     CDS             62935..63303
                     /locus_tag="IYF54_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ9830440.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            63473..63904
                     /gene="mraZ"
                     /locus_tag="IYF54_000068"
     CDS             63473..63904
                     /gene="mraZ"
                     /locus_tag="IYF54_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ9830441.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            63925..64884
                     /gene="rsmH"
                     /locus_tag="IYF54_000069"
     CDS             63925..64884
                     /gene="rsmH"
                     /locus_tag="IYF54_000069"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ9830442.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            64889..65290
                     /gene="ftsL"
                     /locus_tag="IYF54_000070"
     CDS             64889..65290
                     /gene="ftsL"
                     /locus_tag="IYF54_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ9830443.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            65291..67483
                     /locus_tag="IYF54_000071"
     CDS             65291..67483
                     /locus_tag="IYF54_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ9830444.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            67509..68471
                     /locus_tag="IYF54_000072"
     CDS             67509..68471
                     /locus_tag="IYF54_000072"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ9830445.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            68485..69846
                     /locus_tag="IYF54_000073"
     CDS             68485..69846
                     /locus_tag="IYF54_000073"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ9830446.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            69872..70960
                     /gene="murG"
                     /locus_tag="IYF54_000074"
     CDS             69872..70960
                     /gene="murG"
                     /locus_tag="IYF54_000074"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ9830447.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            70975..72195
                     /locus_tag="IYF54_000075"
     CDS             70975..72195
                     /locus_tag="IYF54_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ9830448.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            72351..73676
                     /gene="ftsA"
                     /locus_tag="IYF54_000076"
     CDS             72351..73676
                     /gene="ftsA"
                     /locus_tag="IYF54_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ9830449.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            73699..74940
                     /gene="ftsZ"
                     /locus_tag="IYF54_000077"
     CDS             73699..74940
                     /gene="ftsZ"
                     /locus_tag="IYF54_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ9830450.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            74957..75634
                     /locus_tag="IYF54_000078"
     CDS             74957..75634
                     /locus_tag="IYF54_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ9830451.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            75648..76247
                     /locus_tag="IYF54_000079"
     CDS             75648..76247
                     /locus_tag="IYF54_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ9830452.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            76262..76549
                     /locus_tag="IYF54_000080"
     CDS             76262..76549
                     /locus_tag="IYF54_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ9830453.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            76598..77380
                     /locus_tag="IYF54_000081"
     CDS             76598..77380
                     /locus_tag="IYF54_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ9830454.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            77401..78108
                     /locus_tag="IYF54_000082"
     CDS             77401..78108
                     /locus_tag="IYF54_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ9830455.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            78370..81168
                     /gene="ileS"
                     /locus_tag="IYF54_000083"
     CDS             78370..81168
                     /gene="ileS"
                     /locus_tag="IYF54_000083"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ9830456.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
BASE COUNT        26608 a        13899 c        17301 g        23410 t
ORIGIN      
        1 ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga taaaatacaa
       61 gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa atcgtaatca
      121 aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat agtgcagtcg
      181 cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt gtgccggaca
      241 tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact gtccattttt
      301 ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg ccgcacggaa
      361 aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt aaatttggcg
      421 aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt gacctacacg
      481 tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc atgtatttac
      541 ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt tcagtcggag
      601 caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta attgaaaacg
      661 ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg ggggcaaaag
      721 ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta catggctgtc
      781 ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta gcagctgcag
      841 taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc tttatagcaa
      901 aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa gtttacccct
      961 cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt gccacagatc
     1021 tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa atcatcgata
     1081 cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc gctgatatta
     1141 ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc acagaagtcg
     1201 tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg gcagaaggga
     1261 caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt atcgagaaac
     1321 taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa tgagcgatta
     1381 tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt atgccaaaga
     1441 atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag ctgtgattcg
     1501 tgcacaagca gagaaacaag gattttatta tatggaagga atattggata tcgttggtca
     1561 agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag atatctatat
     1621 ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg caggaaaagc
     1681 ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa gcgttaatgg
     1741 aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac ctctttatcc
     1801 gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa tgatcgatat
     1861 cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa aagcagggaa
     1921 aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg aagtagaatt
     1981 aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac gaagcgtgaa
     2041 aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc gagtttctga
     2101 attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg ttatcttgat
     2161 ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa gcggacgtac
     2221 attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt tcggggcagc
     2281 aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag tcgatacagg
     2341 aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc tagaattaca
     2401 actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca aaaaatcagg
     2461 tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt ggaaacttcg
     2521 aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct ttttgcgtaa
     2581 aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg gtaaaaaaaa
     2641 tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta agatgttaat
     2701 gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag gagcgaaaaa
     2761 atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga ttcaacaact
     2821 ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga atgggaagac
     2881 ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc attctacact
     2941 ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa aaaatacggt
     3001 ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt ttagcagact
     3061 atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt cgtttgatgc
     3121 tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat agttttaatt
     3181 atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt cgaagtaaaa
     3241 aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt ttgttttgtt
     3301 atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata cacgcggatg
     3361 atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg atgctatact
     3421 gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt tatgaggttt
     3481 gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc aaaaggatca
     3541 ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa tgttcaaaaa
     3601 gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga agatggattt
     3661 gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt ttttcataca
     3721 gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat ttttgccatc
     3781 gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact ctccagacga
     3841 ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc agatatcatc
     3901 agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga tagtgaagaa
     3961 aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact ggaattgctc
     4021 atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac catcaagcaa
     4081 aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa atcaaagagg
     4141 cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt gccgggaaaa
     4201 aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta atagacgaag
     4261 cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct gcaaatgaga
     4321 aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt ccatttgtga
     4381 tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg gctgatccca
     4441 actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga gatacagcgt
     4501 ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct ggaaaagcag
     4561 gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca ggttttcttg
     4621 gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca aataaaatta
     4681 gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt ttatcgatct
     4741 taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg gtgttggatg
     4801 ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc ggattactta
     4861 tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact tcgactttct
     4921 cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg gctgcaggaa
     4981 tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa tttaccaaag
     5041 aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt acagaaggtg
     5101 ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg acaggttcag
     5161 cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct catggaggga
     5221 tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc ttggcaattg
     5281 catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca gtgaaagaga
     5341 aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa gcgtaatgtt
     5401 atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag taccagcctt
     5461 caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga gattagaagc
     5521 accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg agtttgttgc
     5581 ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact tggatcatgg
     5641 cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg tgatgatcga
     5701 tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg ttgcgttagc
     5761 tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata atggttctgc
     5821 tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga tttttgtgaa
     5881 agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct tgtatccgaa
     5941 aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac aaatcgatct
     6001 tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg aagcagtaaa
     6061 atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt tcaatgcttt
     6121 gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt atccagcagc
     6181 taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca cagtaggcaa
     6241 ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat agtcttgtca
     6301 taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt cttcttattt
     6361 ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata aagtaaatgg
     6421 ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa ttatctaaaa
     6481 aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag cgaagccacg
     6541 attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt tcgcggtggc
     6601 gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat caccaaatta
     6661 gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat tcgagatgat
     6721 atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa cctagcaaga
     6781 tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt gagtgaatat
     6841 acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc aacaatcaac
     6901 ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat aatgagttgt
     6961 cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc aatggtcaaa
     7021 aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca aaaaattgca
     7081 cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt gacaagtaaa
     7141 gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg tctgtacgga
     7201 taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac cttatctttg
     7261 ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga taatcttact
     7321 tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg
     7381 caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt gttagcttta
     7441 tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta ggggatctgt
     7501 tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt tcagctgcag
     7561 ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg atgaaagtat
     7621 tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc
     7681 aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact attcttatcg
     7741 ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata aactattttt
     7801 actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc tattcatcaa
     7861 gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg actatgtttt
     7921 cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc aatcacatac
     7981 aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc aacgcctaag
     8041 cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg ttcgcggacg
     8101 atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag gccgactaat
     8161 gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa taatcctgct
     8221 gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc gaaaatcctt
     8281 ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa aaatgaccca
     8341 gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac acttgaataa
     8401 gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc aataaaacta
     8461 ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat attttttaca
     8521 ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa ccagataatg
     8581 aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac agcggcagct
     8641 aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc agggatatat
     8701 gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc attcaagaat
     8761 cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt tgaaaggaga
     8821 tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa ctgtttcccc
     8881 atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa acctgcttgg
     8941 ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat caacagataa
     9001 taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt attaaataat
     9061 ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa tttttgtatt
     9121 tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg aataaataaa
     9181 tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg attctcatcc
     9241 actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac taaagaagct
     9301 ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt gttcatccag
     9361 taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat aacgaataaa
     9421 aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt taatatcatt
     9481 agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt ttagatgaaa
     9541 gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg gataaaaatg
     9601 attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg atggaagatt
     9661 cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca aatgatggga
     9721 gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta aaagacgaat
     9781 attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat gcagagtttt
     9841 atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag ctacaacact
     9901 atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc
     9961 tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat gatttattcg
    10021 gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa gaaatcgcca
    10081 aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag
    10141 atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac ttgttagaag
    10201 acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac ctgagtatta
    10261 gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    10321 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tctgagtcac
    10381 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag
    10441 gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct gcatcaagta
    10501 ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga cttatcaccg
    10561 tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac acgatttttg
    10621 tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg
    10681 ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt
    10741 gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca
    10801 gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga
    10861 taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca
    10921 atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct
    10981 acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa
    11041 gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc
    11101 gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt
    11161 tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct
    11221 cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc
    11281 aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt
    11341 tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc
    11401 accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca
    11461 agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc
    11521 ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg
    11581 cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca
    11641 gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg
    11701 ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt
    11761 gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta
    11821 aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca
    11881 tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc
    11941 ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata
    12001 tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat
    12061 aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa
    12121 caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag
    12181 caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg
    12241 gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac
    12301 cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg
    12361 tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa
    12421 gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt
    12481 cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat
    12541 agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac
    12601 cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa
    12661 attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc
    12721 cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa
    12781 cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc
    12841 cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt
    12901 ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat
    12961 ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc
    13021 cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt acgctaggat
    13081 cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag
    13141 aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat
    13201 ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc
    13261 gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta
    13321 aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg
    13381 tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata
    13441 attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc
    13501 cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa
    13561 atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag
    13621 gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc
    13681 caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta
    13741 aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac
    13801 cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat
    13861 ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct
    13921 cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg
    13981 aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga
    14041 aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa
    14101 ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc
    14161 agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg
    14221 tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga
    14281 gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat
    14341 gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa
    14401 tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt
    14461 atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa
    14521 caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca
    14581 tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat
    14641 ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag
    14701 aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg
    14761 ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta
    14821 ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact
    14881 tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg
    14941 cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc
    15001 tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat
    15061 ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga
    15121 gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta
    15181 cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag
    15241 atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg
    15301 aactacgcag taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc
    15361 ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg
    15421 aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc
    15481 tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag
    15541 gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat
    15601 tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg
    15661 tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag
    15721 aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc
    15781 ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct
    15841 ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga
    15901 atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa
    15961 gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa
    16021 gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat
    16081 cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca
    16141 caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt
    16201 tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc
    16261 gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat
    16321 acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa
    16381 cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata
    16441 tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta
    16501 atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta
    16561 tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg
    16621 catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca
    16681 acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca
    16741 catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct
    16801 gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa
    16861 agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct
    16921 gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca
    16981 aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga
    17041 gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat
    17101 gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa
    17161 atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc
    17221 atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat
    17281 tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga
    17341 caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat
    17401 gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa
    17461 agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt
    17521 tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag
    17581 acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat
    17641 cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc
    17701 gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat
    17761 ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag
    17821 aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ttttaggagg
    17881 agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag
    17941 cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa
    18001 ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg
    18061 aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat
    18121 ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt
    18181 ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa
    18241 gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc
    18301 cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt
    18361 caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga
    18421 ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt
    18481 ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt
    18541 tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa
    18601 aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg
    18661 ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa
    18721 cagataaacc agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc
    18781 agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag
    18841 gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag
    18901 gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag
    18961 tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc
    19021 ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag
    19081 aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag
    19141 tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata
    19201 aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc
    19261 tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa
    19321 agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg
    19381 atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa
    19441 aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg
    19501 agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag
    19561 ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg
    19621 aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga
    19681 atggataaga gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca
    19741 ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact
    19801 ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa
    19861 gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt
    19921 tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag
    19981 ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga
    20041 ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt
    20101 ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc
    20161 caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc
    20221 ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata
    20281 caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt
    20341 tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg
    20401 atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc
    20461 caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt
    20521 tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa
    20581 aagaacagac agttacgctg attttttatg agtcacctta tcggatcact gctacattga
    20641 cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg
    20701 tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc
    20761 cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg
    20821 aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg
    20881 aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag
    20941 tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta
    21001 atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata
    21061 atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa
    21121 aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg
    21181 agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag
    21241 tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac
    21301 aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca
    21361 gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag
    21421 ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca
    21481 tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg
    21541 cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag
    21601 gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa
    21661 aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag
    21721 gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct
    21781 attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc
    21841 gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc
    21901 tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac
    21961 atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag
    22021 tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat
    22081 gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc
    22141 tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa
    22201 aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca
    22261 agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca
    22321 caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc
    22381 gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga
    22441 tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc
    22501 ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt
    22561 gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt
    22621 aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat
    22681 atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa
    22741 cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa
    22801 tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa
    22861 aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca
    22921 taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag
    22981 agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc
    23041 tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac
    23101 gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt
    23161 ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac
    23221 catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt
    23281 ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc
    23341 accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat
    23401 tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg
    23461 gtattcacag tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc
    23521 caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata
    23581 ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg
    23641 gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc
    23701 ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc
    23761 tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta
    23821 gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga
    23881 aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc
    23941 tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag
    24001 taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa
    24061 tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca
    24121 cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat
    24181 tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg
    24241 tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat
    24301 cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa
    24361 aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt
    24421 gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat
    24481 agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga
    24541 atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc
    24601 aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc
    24661 aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc
    24721 attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt
    24781 catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc
    24841 atttgctata tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt
    24901 cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt
    24961 ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc
    25021 aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc
    25081 tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg
    25141 gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg
    25201 tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc
    25261 atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg
    25321 ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat
    25381 tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata
    25441 gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga
    25501 aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata
    25561 aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa
    25621 attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga
    25681 agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc
    25741 tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat
    25801 tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt
    25861 gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga
    25921 gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga
    25981 taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga
    26041 ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa
    26101 acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact
    26161 tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc
    26221 ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact
    26281 gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga
    26341 tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt
    26401 aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg
    26461 ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat
    26521 ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag
    26581 caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg
    26641 ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa
    26701 taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag
    26761 aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt attatttttt
    26821 ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt
    26881 ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag
    26941 tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa
    27001 aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca
    27061 tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt
    27121 catggatgat cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt
    27181 ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga
    27241 tcatattgag taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca
    27301 cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa
    27361 tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga
    27421 aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga
    27481 ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg
    27541 ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg
    27601 aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat
    27661 cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat
    27721 atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa
    27781 gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg
    27841 tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa
    27901 ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg
    27961 tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac
    28021 ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct
    28081 cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca
    28141 cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat
    28201 tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac
    28261 aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga
    28321 tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg
    28381 ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt
    28441 atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca
    28501 agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg
    28561 atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa
    28621 aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa
    28681 aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt
    28741 gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat
    28801 tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat
    28861 ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa
    28921 gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga
    28981 tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga
    29041 cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg
    29101 caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta
    29161 tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa
    29221 accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat
    29281 aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag
    29341 cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa
    29401 aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt
    29461 tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt
    29521 attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt
    29581 tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt
    29641 ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt
    29701 gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag
    29761 tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac
    29821 tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc
    29881 gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc
    29941 aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa
    30001 caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac
    30061 aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca
    30121 gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt atctgctcca
    30181 agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca
    30241 gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa
    30301 atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca
    30361 ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc
    30421 tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt
    30481 tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga
    30541 ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat
    30601 tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg
    30661 ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt
    30721 ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca
    30781 tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg
    30841 gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg
    30901 ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag
    30961 ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt
    31021 tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct
    31081 gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg
    31141 tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt
    31201 acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg
    31261 aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat
    31321 gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta
    31381 atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg
    31441 tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg
    31501 atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta
    31561 gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta
    31621 gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa
    31681 ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa
    31741 gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc
    31801 aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc
    31861 gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag
    31921 tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt
    31981 tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc
    32041 ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc
    32101 tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca
    32161 tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc
    32221 cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa
    32281 caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga
    32341 ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa
    32401 agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga
    32461 gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga
    32521 atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag
    32581 caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa
    32641 acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac
    32701 gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc
    32761 agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt
    32821 tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc
    32881 ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc
    32941 caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa
    33001 tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt
    33061 ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta
    33121 tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca
    33181 attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct
    33241 gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca
    33301 caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt
    33361 gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga
    33421 gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc
    33481 aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt
    33541 tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg
    33601 tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc
    33661 cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct
    33721 tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag
    33781 tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc
    33841 agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga
    33901 gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg
    33961 caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt
    34021 gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca
    34081 gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat
    34141 aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat
    34201 acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt
    34261 ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat
    34321 gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt
    34381 gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat
    34441 atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt
    34501 tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc
    34561 cggaagaaag catgcagcag cacttattga gttttctagt cgataattta cttttattcg
    34621 cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa
    34681 gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt
    34741 tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa
    34801 tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg
    34861 tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg
    34921 tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt
    34981 tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag
    35041 atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag
    35101 caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag
    35161 aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg
    35221 taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat
    35281 gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga
    35341 caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta
    35401 cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc
    35461 attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc
    35521 ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact
    35581 ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat
    35641 taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg
    35701 ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt
    35761 atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc
    35821 tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg
    35881 cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag
    35941 aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg
    36001 cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac
    36061 cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat
    36121 gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat
    36181 acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc
    36241 ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg
    36301 cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca
    36361 cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat
    36421 ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga
    36481 acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac
    36541 aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt
    36601 cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa
    36661 attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg
    36721 aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag
    36781 tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat
    36841 ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta
    36901 ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta
    36961 tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa
    37021 ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct
    37081 attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa
    37141 gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt
    37201 aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta
    37261 tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac
    37321 aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct
    37381 aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga
    37441 tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag
    37501 ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct
    37561 tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc
    37621 agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt
    37681 cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt
    37741 ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt
    37801 agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc
    37861 gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct
    37921 acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg
    37981 cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa
    38041 gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac
    38101 cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag
    38161 ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa
    38221 atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt
    38281 gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata
    38341 gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa
    38401 ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga
    38461 taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc
    38521 acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc
    38581 ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg
    38641 tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga
    38701 atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc
    38761 cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag
    38821 aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata
    38881 cgtaaataaa aagattcttt tttattatat atgttattaa aaaaagacat aaaggtattc
    38941 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
    39001 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
    39061 atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa
    39121 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
    39181 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
    39241 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
    39301 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
    39361 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
    39421 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
    39481 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
    39541 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
    39601 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
    39661 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
    39721 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
    39781 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
    39841 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
    39901 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
    39961 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
    40021 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
    40081 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
    40141 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
    40201 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
    40261 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
    40321 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
    40381 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
    40441 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
    40501 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
    40561 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
    40621 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
    40681 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
    40741 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
    40801 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
    40861 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
    40921 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
    40981 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
    41041 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
    41101 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
    41161 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
    41221 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
    41281 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
    41341 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
    41401 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
    41461 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
    41521 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
    41581 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
    41641 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
    41701 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
    41761 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
    41821 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
    41881 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
    41941 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
    42001 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
    42061 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
    42121 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
    42181 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
    42241 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
    42301 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
    42361 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
    42421 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
    42481 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
    42541 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
    42601 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
    42661 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
    42721 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
    42781 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
    42841 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
    42901 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
    42961 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
    43021 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
    43081 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
    43141 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
    43201 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
    43261 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
    43321 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
    43381 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
    43441 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
    43501 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
    43561 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
    43621 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
    43681 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
    43741 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
    43801 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
    43861 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
    43921 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
    43981 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
    44041 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
    44101 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
    44161 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
    44221 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
    44281 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
    44341 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
    44401 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
    44461 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
    44521 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
    44581 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
    44641 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
    44701 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
    44761 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
    44821 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
    44881 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
    44941 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
    45001 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
    45061 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
    45121 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
    45181 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
    45241 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
    45301 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
    45361 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
    45421 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
    45481 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
    45541 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
    45601 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
    45661 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
    45721 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
    45781 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
    45841 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
    45901 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
    45961 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
    46021 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
    46081 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
    46141 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
    46201 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
    46261 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
    46321 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
    46381 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
    46441 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
    46501 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
    46561 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
    46621 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
    46681 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
    46741 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
    46801 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
    46861 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
    46921 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
    46981 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
    47041 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
    47101 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
    47161 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
    47221 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
    47281 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
    47341 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
    47401 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
    47461 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
    47521 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
    47581 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
    47641 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
    47701 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
    47761 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
    47821 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
    47881 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
    47941 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
    48001 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
    48061 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
    48121 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
    48181 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
    48241 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
    48301 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
    48361 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
    48421 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
    48481 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
    48541 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
    48601 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
    48661 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
    48721 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
    48781 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
    48841 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
    48901 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
    48961 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
    49021 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
    49081 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    49141 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    49201 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    49261 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    49321 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    49381 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    49441 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    49501 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    49561 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    49621 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    49681 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    49741 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    49801 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    49861 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    49921 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    49981 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    50041 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    50101 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    50161 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    50221 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    50281 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    50341 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    50401 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    50461 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    50521 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    50581 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    50641 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    50701 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    50761 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    50821 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    50881 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    50941 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    51001 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    51061 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    51121 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    51181 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    51241 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    51301 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    51361 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    51421 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    51481 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    51541 tcgtattagc tgcccttgta gaaagatggg tatcaatcaa attcttacca gttgtatcaa
    51601 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    51661 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    51721 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    51781 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    51841 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    51901 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    51961 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    52021 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    52081 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    52141 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    52201 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    52261 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    52321 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    52381 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    52441 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    52501 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    52561 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    52621 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    52681 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    52741 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    52801 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    52861 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    52921 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    52981 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    53041 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    53101 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    53161 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    53221 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    53281 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    53341 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    53401 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    53461 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    53521 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    53581 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    53641 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    53701 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    53761 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    53821 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    53881 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    53941 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    54001 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    54061 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    54121 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    54181 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    54241 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    54301 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    54361 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    54421 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    54481 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    54541 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    54601 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    54661 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    54721 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    54781 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    54841 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    54901 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    54961 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    55021 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    55081 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    55141 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    55201 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    55261 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    55321 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    55381 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    55441 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    55501 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    55561 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    55621 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    55681 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    55741 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    55801 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    55861 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    55921 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    55981 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    56041 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    56101 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    56161 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    56221 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    56281 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    56341 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    56401 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    56461 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    56521 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    56581 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    56641 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    56701 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    56761 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    56821 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    56881 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    56941 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    57001 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    57061 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    57121 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    57181 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    57241 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    57301 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    57361 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    57421 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    57481 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    57541 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    57601 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    57661 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    57721 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    57781 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    57841 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    57901 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    57961 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    58021 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    58081 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgactc
    58141 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    58201 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    58261 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    58321 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    58381 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    58441 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    58501 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    58561 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    58621 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    58681 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    58741 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    58801 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    58861 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    58921 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    58981 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    59041 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    59101 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    59161 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    59221 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    59281 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    59341 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    59401 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    59461 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    59521 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    59581 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    59641 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    59701 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    59761 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    59821 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    59881 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    59941 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    60001 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    60061 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    60121 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    60181 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    60241 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    60301 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    60361 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    60421 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    60481 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    60541 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    60601 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    60661 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    60721 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    60781 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    60841 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    60901 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    60961 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    61021 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    61081 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    61141 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    61201 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    61261 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    61321 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    61381 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    61441 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    61501 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    61561 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    61621 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    61681 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    61741 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    61801 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    61861 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    61921 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    61981 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    62041 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    62101 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    62161 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    62221 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    62281 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    62341 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    62401 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    62461 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    62521 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    62581 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    62641 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    62701 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    62761 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    62821 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    62881 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    62941 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    63001 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    63061 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    63121 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    63181 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    63241 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    63301 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    63361 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    63421 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    63481 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    63541 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    63601 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    63661 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    63721 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    63781 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    63841 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    63901 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    63961 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    64021 ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat
    64081 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    64141 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    64201 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    64261 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    64321 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    64381 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    64441 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    64501 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    64561 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    64621 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    64681 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    64741 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    64801 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    64861 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    64921 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    64981 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    65041 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    65101 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    65161 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    65221 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    65281 agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa
    65341 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggaatgtt
    65401 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    65461 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    65521 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    65581 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    65641 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    65701 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    65761 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    65821 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    65881 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    65941 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    66001 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    66061 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    66121 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    66181 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    66241 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    66301 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    66361 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    66421 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    66481 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    66541 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    66601 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    66661 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    66721 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    66781 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    66841 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaac
    66901 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    66961 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    67021 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    67081 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    67141 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    67201 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    67261 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    67321 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    67381 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    67441 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    67501 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    67561 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    67621 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    67681 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    67741 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    67801 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    67861 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    67921 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    67981 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    68041 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    68101 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    68161 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    68221 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    68281 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    68341 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    68401 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    68461 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    68521 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    68581 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    68641 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    68701 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    68761 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    68821 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    68881 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    68941 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    69001 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    69061 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    69121 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    69181 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    69241 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    69301 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    69361 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    69421 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    69481 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    69541 gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    69601 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    69661 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    69721 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    69781 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    69841 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    69901 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    69961 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    70021 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    70081 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    70141 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    70201 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    70261 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    70321 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    70381 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    70441 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    70501 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    70561 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    70621 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    70681 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    70741 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    70801 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    70861 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    70921 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    70981 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    71041 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    71101 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    71161 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    71221 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    71281 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    71341 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    71401 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    71461 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    71521 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    71581 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    71641 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    71701 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    71761 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    71821 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    71881 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    71941 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    72001 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    72061 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    72121 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    72181 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    72241 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    72301 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    72361 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    72421 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    72481 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    72541 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    72601 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    72661 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    72721 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    72781 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    72841 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    72901 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    72961 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    73021 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    73081 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    73141 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    73201 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    73261 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    73321 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    73381 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    73441 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    73501 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    73561 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    73621 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    73681 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    73741 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    73801 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    73861 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    73921 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    73981 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    74041 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    74101 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    74161 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    74221 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    74281 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    74341 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    74401 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    74461 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    74521 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    74581 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    74641 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    74701 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    74761 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    74821 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    74881 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    74941 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    75001 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    75061 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    75121 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    75181 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    75241 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    75301 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    75361 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    75421 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    75481 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    75541 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    75601 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    75661 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    75721 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    75781 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    75841 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    75901 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    75961 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    76021 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    76081 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    76141 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    76201 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    76261 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    76321 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    76381 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    76441 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    76501 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    76561 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    76621 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    76681 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    76741 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    76801 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    76861 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    76921 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    76981 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    77041 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    77101 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    77161 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    77221 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    77281 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    77341 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    77401 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    77461 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    77521 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    77581 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    77641 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    77701 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    77761 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    77821 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    77881 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    77941 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    78001 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    78061 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    78121 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    78181 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    78241 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    78301 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    78361 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    78421 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    78481 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    78541 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    78601 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    78661 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    78721 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    78781 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    78841 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac
    78901 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    78961 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    79021 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    79081 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    79141 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    79201 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    79261 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    79321 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    79381 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    79441 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    79501 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    79561 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    79621 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    79681 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    79741 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    79801 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    79861 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    79921 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    79981 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    80041 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    80101 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    80161 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    80221 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    80281 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    80341 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    80401 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    80461 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    80521 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    80581 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    80641 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    80701 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    80761 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    80821 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    80881 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    80941 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    81001 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    81061 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    81121 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    81181 ggtgtgacat gttctttgtc acaccccctt ttgttatc
//
LOCUS       DACIWG010000002        79173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.002, whole genome shotgun sequence.
ACCESSION   DACIWG010000002 DACIWG010000000
VERSION     DACIWG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.002"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(38..316)
                     /locus_tag="IYF54_000084"
                     /pseudo
     CDS             complement(38..316)
                     /locus_tag="IYF54_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(519..998)
                     /gene="rlmH"
                     /locus_tag="IYF54_000085"
     CDS             complement(519..998)
                     /gene="rlmH"
                     /locus_tag="IYF54_000085"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ9830457.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1426..2715
                     /locus_tag="IYF54_000086"
     CDS             1426..2715
                     /locus_tag="IYF54_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ9830458.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2754..3359)
                     /locus_tag="IYF54_000087"
     CDS             complement(2754..3359)
                     /locus_tag="IYF54_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ9830459.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3555..4019)
                     /locus_tag="IYF54_000088"
     CDS             complement(3555..4019)
                     /locus_tag="IYF54_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9830460.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4041..4361)
                     /locus_tag="IYF54_000089"
     CDS             complement(4041..4361)
                     /locus_tag="IYF54_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ9830461.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="IYF54_000090"
     CDS             complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="IYF54_000090"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ9830462.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5783..6133
                     /locus_tag="IYF54_000091"
     CDS             5783..6133
                     /locus_tag="IYF54_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9830463.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            6318..7271
                     /locus_tag="IYF54_000092"
     CDS             6318..7271
                     /locus_tag="IYF54_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9830464.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            7284..9071
                     /locus_tag="IYF54_000093"
     CDS             7284..9071
                     /locus_tag="IYF54_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830465.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            9058..10260
                     /locus_tag="IYF54_000094"
     CDS             9058..10260
                     /locus_tag="IYF54_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830466.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="IYF54_000095"
     CDS             complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="IYF54_000095"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ9830467.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11903..12748
                     /locus_tag="IYF54_000096"
     CDS             11903..12748
                     /locus_tag="IYF54_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ9830468.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12752..13192
                     /locus_tag="IYF54_000097"
     CDS             12752..13192
                     /locus_tag="IYF54_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ9830469.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            13242..13436
                     /locus_tag="IYF54_000098"
     CDS             13242..13436
                     /locus_tag="IYF54_000098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830470.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13572..14258
                     /locus_tag="IYF54_000099"
     CDS             13572..14258
                     /locus_tag="IYF54_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9830471.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14666..15355)
                     /locus_tag="IYF54_000100"
     CDS             complement(14666..15355)
                     /locus_tag="IYF54_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830472.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15330..17459)
                     /locus_tag="IYF54_000101"
     CDS             complement(15330..17459)
                     /locus_tag="IYF54_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ9830473.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17507..18844)
                     /locus_tag="IYF54_000102"
     CDS             complement(17507..18844)
                     /locus_tag="IYF54_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ9830474.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFCGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="IYF54_000103"
     CDS             complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="IYF54_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ9830475.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="IYF54_000104"
     CDS             complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="IYF54_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ9830476.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19780..20304)
                     /gene="infC"
                     /locus_tag="IYF54_000105"
     CDS             complement(19780..20304)
                     /gene="infC"
                     /locus_tag="IYF54_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ9830477.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            20624..21256
                     /locus_tag="IYF54_000106"
     CDS             20624..21256
                     /locus_tag="IYF54_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ9830478.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(21258..22163)
                     /locus_tag="IYF54_000107"
     CDS             complement(21258..22163)
                     /locus_tag="IYF54_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9830479.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(22248..22901)
                     /locus_tag="IYF54_000108"
     CDS             complement(22248..22901)
                     /locus_tag="IYF54_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9830480.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            23223..24167
                     /gene="mvk"
                     /locus_tag="IYF54_000109"
     CDS             23223..24167
                     /gene="mvk"
                     /locus_tag="IYF54_000109"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ9830481.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24160..25137
                     /gene="mvaD"
                     /locus_tag="IYF54_000110"
     CDS             24160..25137
                     /gene="mvaD"
                     /locus_tag="IYF54_000110"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ9830482.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            25150..26235
                     /locus_tag="IYF54_000111"
     CDS             25150..26235
                     /locus_tag="IYF54_000111"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ9830483.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            26232..27296
                     /locus_tag="IYF54_000112"
     CDS             26232..27296
                     /locus_tag="IYF54_000112"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ9830484.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27545..28348)
                     /locus_tag="IYF54_000113"
     CDS             complement(27545..28348)
                     /locus_tag="IYF54_000113"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ9830485.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="IYF54_000114"
     CDS             complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="IYF54_000114"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ9830486.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(29226..30233)
                     /locus_tag="IYF54_000115"
     CDS             complement(29226..30233)
                     /locus_tag="IYF54_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ9830487.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(30240..30884)
                     /locus_tag="IYF54_000116"
     CDS             complement(30240..30884)
                     /locus_tag="IYF54_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9830488.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30984..31742)
                     /locus_tag="IYF54_000117"
     CDS             complement(30984..31742)
                     /locus_tag="IYF54_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ9830489.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="IYF54_000118"
     CDS             complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="IYF54_000118"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ9830490.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="IYF54_000119"
     CDS             complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="IYF54_000119"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ9830491.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="IYF54_000120"
     CDS             complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="IYF54_000120"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ9830492.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="IYF54_000121"
     CDS             complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="IYF54_000121"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ9830493.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="IYF54_000122"
     CDS             complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="IYF54_000122"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ9830494.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            37140..38009
                     /locus_tag="IYF54_000123"
     CDS             37140..38009
                     /locus_tag="IYF54_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9830495.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(38135..39034)
                     /locus_tag="IYF54_000124"
     CDS             complement(38135..39034)
                     /locus_tag="IYF54_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9830496.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(39199..40023)
                     /locus_tag="IYF54_000125"
     CDS             complement(39199..40023)
                     /locus_tag="IYF54_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9830497.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(40034..41329)
                     /locus_tag="IYF54_000126"
     CDS             complement(40034..41329)
                     /locus_tag="IYF54_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ9830498.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(41345..41824)
                     /locus_tag="IYF54_000127"
     CDS             complement(41345..41824)
                     /locus_tag="IYF54_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ9830499.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41830..42858)
                     /locus_tag="IYF54_000128"
     CDS             complement(41830..42858)
                     /locus_tag="IYF54_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ9830500.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(42875..43981)
                     /locus_tag="IYF54_000129"
     CDS             complement(42875..43981)
                     /locus_tag="IYF54_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ9830501.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="IYF54_000130"
                     /gene_synonym="adhC"
     CDS             complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="IYF54_000130"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ9830502.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="IYF54_000131"
     CDS             complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="IYF54_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ9830503.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="IYF54_000132"
     CDS             complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="IYF54_000132"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ9830504.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            48952..49650
                     /locus_tag="IYF54_000133"
     CDS             48952..49650
                     /locus_tag="IYF54_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ9830505.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(49818..52457)
                     /locus_tag="IYF54_000134"
     CDS             complement(49818..52457)
                     /locus_tag="IYF54_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9830506.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(53076..54107)
                     /gene="queA"
                     /locus_tag="IYF54_000135"
     CDS             complement(53076..54107)
                     /gene="queA"
                     /locus_tag="IYF54_000135"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ9830507.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            54300..55475
                     /locus_tag="IYF54_000136"
     CDS             54300..55475
                     /locus_tag="IYF54_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9830508.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            55479..56120
                     /locus_tag="IYF54_000137"
     CDS             55479..56120
                     /locus_tag="IYF54_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830509.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            56117..57034
                     /locus_tag="IYF54_000138"
     CDS             56117..57034
                     /locus_tag="IYF54_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9830510.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            57031..57702
                     /locus_tag="IYF54_000139"
     CDS             57031..57702
                     /locus_tag="IYF54_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830511.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(58243..58731)
                     /locus_tag="IYF54_000140"
     CDS             complement(58243..58731)
                     /locus_tag="IYF54_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ9830512.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(58932..59198)
                     /locus_tag="IYF54_000141"
     CDS             complement(58932..59198)
                     /locus_tag="IYF54_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ9830513.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(59198..59461)
                     /locus_tag="IYF54_000142"
     CDS             complement(59198..59461)
                     /locus_tag="IYF54_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9830514.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(59454..59726)
                     /locus_tag="IYF54_000143"
     CDS             complement(59454..59726)
                     /locus_tag="IYF54_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830515.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            59937..60122
                     /locus_tag="IYF54_000144"
     CDS             59937..60122
                     /locus_tag="IYF54_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9830516.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(60264..62087)
                     /locus_tag="IYF54_000145"
     CDS             complement(60264..62087)
                     /locus_tag="IYF54_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ9830517.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(62685..65390)
                     /locus_tag="IYF54_000146"
     CDS             complement(62685..65390)
                     /locus_tag="IYF54_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ9830518.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(65696..66643)
                     /locus_tag="IYF54_000147"
     CDS             complement(65696..66643)
                     /locus_tag="IYF54_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ9830519.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            66832..67101
                     /gene="rpsN"
                     /locus_tag="IYF54_000148"
     CDS             66832..67101
                     /gene="rpsN"
                     /locus_tag="IYF54_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ9830520.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(67277..67639)
                     /locus_tag="IYF54_000149"
     CDS             complement(67277..67639)
                     /locus_tag="IYF54_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ9830521.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(67650..68771)
                     /locus_tag="IYF54_000150"
     CDS             complement(67650..68771)
                     /locus_tag="IYF54_000150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ9830522.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(68787..69137)
                     /locus_tag="IYF54_000151"
     CDS             complement(68787..69137)
                     /locus_tag="IYF54_000151"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ9830523.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(69267..70778)
                     /locus_tag="IYF54_000152"
     CDS             complement(69267..70778)
                     /locus_tag="IYF54_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9830524.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(71100..72452)
                     /locus_tag="IYF54_000153"
     CDS             complement(71100..72452)
                     /locus_tag="IYF54_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ9830525.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(72605..73681)
                     /locus_tag="IYF54_000154"
     CDS             complement(72605..73681)
                     /locus_tag="IYF54_000154"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ9830526.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            73877..75622
                     /locus_tag="IYF54_000155"
     CDS             73877..75622
                     /locus_tag="IYF54_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ9830527.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(75902..76339)
                     /locus_tag="IYF54_000156"
     CDS             complement(75902..76339)
                     /locus_tag="IYF54_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ9830528.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(76323..77696)
                     /locus_tag="IYF54_000157"
     CDS             complement(76323..77696)
                     /locus_tag="IYF54_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830529.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(78018..78506)
                     /locus_tag="IYF54_000158"
     CDS             complement(78018..78506)
                     /locus_tag="IYF54_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9830530.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(78528..79130)
                     /locus_tag="IYF54_000159"
     CDS             complement(78528..79130)
                     /locus_tag="IYF54_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9830531.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22691 a        16795 c        14460 g        25227 t
ORIGIN      
        1 atattcttta ggatattaaa attaactagc accacgggta gctattaagt aaagcatcaa
       61 aataattgat agcaactttg gtatcatcat tagaatctat ataccttttc aaaaacattt
      121 taaaaaactc atcagataaa ttgtgggttt catttggagc aaacaaaaaa gctaaaaaat
      181 tactatgtct tatttcattt ttagatagct taagtatctg aaatatattt gttttcctag
      241 tccatttaga tagtttttct tcaatatcat ccatatcctc caatagattt tttaaacact
      301 ttttttcatc tatcatgttt tttctccttg ccattattct aactaaaatt ttacgtattt
      361 gtttctatgt ctacattatc tttttaacag tatctatttt tcttttgcaa cttacttggc
      421 taccaaagca aataaaaaag ttatttataa atttctccct accactacct attgctatta
      481 tttaaaatag ggataatttt ttctcttaaa aaatatcatt atttgtggta tggagccccc
      541 gcgttgatcc ggaaggcacg gtagatttgc tcgacgagga tcaggcgcat caattgatgt
      601 ggatacgtca ttttgccaaa agagatctga gcattgcttc ttttcatgac ggcttcgctg
      661 agtcctaaag aaccgccgat aacaaaaaca aggtggcttt ttccttggat acctaattgg
      721 tcgatttgtt tagcaaaaga ttcgcttgtg ggatttttcc cttcaattgc taagccatag
      781 acgtattctt gttctttgat tttcgctaag atccgttctc cttctttttc ttttacttgt
      841 ctcatttgcg cttcgcttaa attctccggg gctttttcgt caggaacttc gatcaattcg
      901 attttcgcat aggcattcag ccgtttgaca tactcattga taccttggat caagtatttt
      961 tcttttaatt ttcctactga aataattttg atattcatgt tttctctcct ttgatcttca
     1021 cgctcacttt gtttattata atcaattttt tcgtctatat gtttattttg actatttttg
     1081 ttaaaaagat ttccccaaca gttatgcaca acatccacag gtttatccac agaaaacagc
     1141 gttgttttac cttatttcat cacattcatt aggcaaacat acgttttata agaatttatc
     1201 cactttatac acaggctgtg gatatgtttt atcccttatt tttccaatcc tatccgcgta
     1261 gttttacaca atatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt
     1321 ttcctaaagt ttttcataaa aagttcaaag tctcaaagta gactaaagtc aacaaataag
     1381 actatactaa cagtagataa atgaatgtaa ttggaggagg aacacatgga tagaaaaaat
     1441 gtgacaccaa agatgaaaaa aaataaaaat aatagcctct ggcgtaagtt gggtctgggg
     1501 cttgttggag ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc
     1561 actggcaaca atagttctac tgctacaagc ggcaatcaaa attctgccgg tgaaacagtt
     1621 gtggaaaatg tcaaagtcaa tgtagattct gatatcacca aagcggtaga taaagttcaa
     1681 gacgctgtcg tttctgttat caacttacaa agtcaaaacc aatcaagcgg ttttggcgga
     1741 ttattcgggc agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt
     1801 gaaggaagcg gcgtaatcta caaaaaagac gggaatactg cttatgtagt cacaaacaat
     1861 cacgtagtag atggacagca aggtttggaa gtcatgatga aagacggtac gaaggtaaaa
     1921 gctgaattag ttggaacaga tgcctattca gatttagcgg tgttgaagat caattcagac
     1981 aaagtcgaaa cggttgcctc ttttggggac tcaagtgctt tgaaggttgg tgaacctgcg
     2041 attgcgattg gttccccatt aggatctgaa tatgcgaatt ctgtcacttc aggaatcatt
     2101 tcttccttga accgacaagt aacaagtacg aatgaatcga acgaaacagt caatatcaat
     2161 gcgatccaaa cagatgcagc gatcaacccg ggaaattctg gtggtccttt agtgaatatc
     2221 gaaggacaag ttatcggtat caactcaagt aagatcgcaa gtacttctga atcgacttct
     2281 aatgtaagcg tagaaggaat gggatttgcc atcccaagta acgatgtagt gaatatcatc
     2341 aaccaactgg aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac
     2401 ttatctgccg tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact
     2461 aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg cagaaaaagc tgggttgaaa
     2521 caatacgacg tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa
     2581 tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg
     2641 aaagaaaaaa caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa
     2701 aatagtgaaa attaataaaa aagaggttgt gacaaagact tgtcacaacc tctttatttt
     2761 ggaaatactt ctccgactac ggcatcctca ggcaatacta agattccttt tttttctgga
     2821 gcatttggca aatgcaattc acgagcagaa caaatcattc catagctggc tacccctcgc
     2881 aaagttcctg gccagatcat cattccatct ggcatcatgg ctcctggttt tgcaacgaca
     2941 actttttgtc cagcttcgat atttggtgca ccgcaaacaa tttgcaatac tgtcccattg
     3001 tccacttctg tctcagtaat cgataaatgg tcagagtcag gatgcgggac gcatgttttt
     3061 acatacccga caacgatctt cggttcatga tcagcttcaa gctgttcttc gaatcctgct
     3121 tctttaatca atcgattcaa tttcgctaat tcttcatctg ttaagcttac ttggccgttc
     3181 ccttcaattg ttagatgatc ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt
     3241 tcatctttga tccgagcaac attccattta cgttcgacag tgttttctgt tcctttatcg
     3301 tcggctatga taaccatcaa agtgtctccg acgtgtgctt tgttataagc aaaaatcatg
     3361 ttctttcctc cattatcctt tgctgagatt tatcttaaca aaaaattgca ttcactgcat
     3421 gagatttttt tcatgcttta tttccgaaga agcttcttct ggaacaccat ttattcgtaa
     3481 caagggactg tgacaagact tttgtcacag tccctttact gtttgattaa aaaggttata
     3541 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
     3601 caaggtgaat gacgtatgac gtagctggct acacttcctg tcataaagcg ttcgactgca
     3661 tttagtccag actgcccaac catgattaga tcgattgcat atttttttgg caatttgact
     3721 gccatggatt cacgaacaga gccgtatgca ataattcctt ctatatttgt gtaatcaaca
     3781 gattttccat attctttaca ttcttcgatc atttgcttgg ctgtttcagt ttgctgatcg
     3841 actagatttt gatcaatagg agcatagccc ataaaattat aaaattgctg atcgattacg
     3901 tttgctacat atactttccc attattacgg cgagccactt ctaacgcttt tttgaaagca
     3961 gcaagtgctt gttcactacc gtcaatacct actaaaatgt tcttatattc ttgattcatc
     4021 ttgacccctc ctaacagatg tcagcctttt attacacttt ctaggaacga ttcgatttcc
     4081 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
     4141 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
     4201 tctacttcaa taaattgcca atcagaaaag tcattttcga tttcaggcat ttgcggtttg
     4261 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactttt tccagtttct
     4321 acgtagcctg ctaattcttc taaactattt gggataatca ttctttttcc tctactttct
     4381 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
     4441 ctttttataa aaaaaatctg acaaaaggtt gcttttgcca gattttatag tttcactatc
     4501 gcgttatttc ccgtatacga tcgtgttcac tgttgattta tctaaacctt tcaatgtttg
     4561 aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg
     4621 gatataacgt cccactacgc caatcactgt acttgggatt ccttcatttt gtgtatgagc
     4681 agcacctgca tctgtaccgc cttttgaaac aaagtattga taagggatgt tgtttgtttc
     4741 agcgatatct agcaagtact cacgcaaacg aggaagcatg atcaaacctg gatcttggat
     4801 acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca tttttcgtca ctgtgtcatc
     4861 tgctgcagag cagtctactg caaagaacag atctggcttg aatttattga cagaagcttt
     4921 agaaccacgc aagcctactt cttcttggac attcgcgcct gcaatcaatg tatgacctaa
     4981 tgtttcattt tgtaacgctt ctaaagcttc taataccatt gtacagccat agcggttgtc
     5041 ccacgatttg ctgatgatat tcttcccgtt ggctgttttg atcgtttcag cgtaaggaac
     5101 gattgtgtct cctggaagaa caccgtaact cattgcctct tctcttgatt caaacccagc
     5161 atcgaacaaa atatctgtta cttctactga tttttgtccg cttgttcctc gtaataaatg
     5221 tgggggaacg gaagaagaaa tacatggata attcttacca gtagaggttt taagtgtaaa
     5281 acgttgagca gaaacaacgt aagggttcca tccgcctaga ggtacgactt taaaaagccc
     5341 gttttcttgg atttgtgtca gcatgaatcc gacttcatcc atatgtgctg cgaccattac
     5401 acgaggtgca tccgcttctt catggtggcg cagcccaaaa atgccgccca aaccatctaa
     5461 ctgtaactca tctaccagcg gttccatatg actttccata taggcgcgaa cgtcttgttc
     5521 aaaaccgctt gttccttgta attctgttaa ctctttgatt cgttgaaatg ttttttcttc
     5581 catatgctat tcgtctaaat ttcagactaa actcctctca gacttatatt gatctgcgga
     5641 taaaatatga ttttttctgc agaccgatcg tatttattat agcttatttt ttctggtagc
     5701 ggttatttat ttcaaagaaa atatgagagt gtggtaaaat aaacacaaag agtagcaagt
     5761 gagaagaagg aggtatatcc acatgtatga agaaagcgaa ttaagctatt ttaaaggcgg
     5821 gttagccgtt gggatcggtc taggattatt gagtggggta gcttcaacga tttggtatca
     5881 tcgcaaaaaa gtgttatcag cagaccaagt gcttgaaaat atcaaaacat cttttttaaa
     5941 agaaggcgca atcgaaggtg cttggatctc ttttgaaaaa gaacctattc ggaaattcgc
     6001 cgttcggtcg agtgcttatc ggggtgggct ttcccggata gaagatggcg aaactgttta
     6061 ttatgaattt ttagcagatg ctcatacagg aaccgtatta gacatttcaa gaaaaaaggg
     6121 cgcaatagat taatacgtca aaaaaatgaa ataacgaata cgcacccact atctaaaaga
     6181 gaggtatcct atccttcttt tatagtgggt gatttttata aaaatagggc tatttcctct
     6241 tggaatagct agtatttgta gttattatac ggtattattg aaataagatt agttttatta
     6301 tataaggaga gtgacaaatg gaatcacgtg aaaaaattgc cgtgctcatt ccttgttata
     6361 acgaagaggc gacaatttca actgttattg aagattttaa aagggaatta ccagaagcag
     6421 atatttatgt ctatgataat aattcgaccg atcgaacata cgaattggcc gttcaaggcg
     6481 gagcaatcgt aaagaaagaa ccacgtcaag gaaaagggaa tgtaatccgt caaatgtttt
     6541 ttgatattga tgccgattat tatttgatgg tagatggtga tgatacttat cctgcagaag
     6601 ctgcacatgg tttgcttgag aaattacgtt ctggcgaagc agatatggtc attggcgatc
     6661 gtctatcaaa tggaacctat tttgatgaaa ataagcgtcc tttccatgat tttgggaata
     6721 atctggttaa aaatacaatt acttacttat ataaaacaaa aattcgcgat gtcatgaccg
     6781 gctatcgagg attcaatcga atttttgtca aaagcttccc tattatgagt tctggttttc
     6841 aaatcgaaac agaattgacg attcatgctt tagataaaaa attcaagttc gttgagattc
     6901 caatcgatta tcgcgatcgt cctgaaggta gcgaatcaaa attgaataca ttctctgacg
     6961 gattcaaagt gatcatgatg atcatcaaaa tgtgcaaaga ttataaacct ttgcttttct
     7021 ttgggatctg gaccatcttc ttctttttat ttggattggt catggggatt cctgtgattc
     7081 gtgaatatat gctgacaagc tttattacac gcatcccttc tgcgatttta agtactggat
     7141 tgatgacttt agcccttctt tcattagtaa cagggttgat tttggatacg gtcgtcacaa
     7201 atgccaagaa agaatatgaa ttgaatttat accatgtcta ccacgaatat caaaataaag
     7261 aaatgaagta ggaggccttt tttatgcata agaaaaagga gaatctattt cctacttttt
     7321 ttgcaacttt atttgcgtca ggttttgctt tttttacttc ttatggtcta ggaactttga
     7381 atccaacagt aatcattggt ttagccatgg ttttgctact tgcttttaag caatatggaa
     7441 tagagttgtt ttcaataaaa aacaaagttg cagctattgc ccgattgttt ttttctgcag
     7501 cttttgctct tacactttct attggcagac agatacaatt tcagtatgtc tcagcaaact
     7561 atacaaagac aacgattgct gatggaagta ttttttactt tcttgtttat ttctccattt
     7621 ttgtgtattc ttttttattt ttacaatcga tttatttatc aatgccaaaa ctggaaacat
     7681 tttttactgc tcaaaacaca caggctttct cttcaagaaa atatttttta ttctttactg
     7741 tctttttagt tctgtgttgg cttccttatc tgttggcatt gtatcctgga gtggttttgc
     7801 ctgattcgtt aagttcggtc gcccaatcta tgggtgatgt tcctataagc aatcatcatc
     7861 cagtcttatt caccttactg gtcaagctat ttgttcatga tttaccaact gatacgatca
     7921 atcacggtgt atttttattt tcgatttttc aaacattgat aacagcttca gcgataagtt
     7981 attctctttg ctggctgtta aaaagaaaca tccatttttt tccggtactc attagtttat
     8041 gctattttgc atttgcaccg gtttttccaa tctacgcctt gaacatgcaa aaagatactt
     8101 tgttctctat cgcttgcttt ttattttcct tagcggtttt tgactttatt tttcaacgag
     8161 aaaagccaac gattctcaat cagatttacg taatcatcct tgctttactg accacctatc
     8221 taagaaataa tgggatttat gtagtaaccg gcacaggttt gatttgcggc ttgattattt
     8281 tgaaacaaaa aaggtatcaa gcattctttc aagtggtgct gggttataca gtgattgctg
     8341 gactactcat ccacccgttg atggctcgtt atgctattcc tacagaaact gctgaacaat
     8401 taggtgttcc tttgcagcaa atttctagaa ctattgttat ggatggtgac ttgtctaaga
     8461 aagataaagc attcatgaat cagctactcc cgataaaaga ttatgaagcg tattctccta
     8521 gtcttgcaga ctcaatcaag tggcataaaa attttaacaa tgagtttttg aatacacata
     8581 aaaaagaatt tctatcacta tgggcaagaa atttaccaag taatataaaa atctatacag
     8641 atgcttatat tttagaaacg ttcgggttct gggtacctgg tacaaaaaat agttacggct
     8701 ttttagatac tcgtgtaaac acgaataatt atggtatgaa gcaagtagat ttgattgaac
     8761 atcttactgg attgtcagga atgaaaaaaa tcatcgatgc tcgtgatttc tttggttccg
     8821 gcacattact gtggttagct atcggcagtg cacttttagc aatctttaag aaaaaatatc
     8881 ggtatttact cctttttctt cccggtatgc ttgtttttgc tactattatg attgctactc
     8941 ctgtggcttt tagtttacgg tatgtgtttg ttttagcatt aggtctacct gtttatatcc
     9001 tctctccttt tttactagat aaaagagaaa aaatgaaaac agatatagga gatactcatg
     9061 aaactattta aaaagatatt ttttgctttt gttctcttga tcgctagcta ttgcgtacta
     9121 ctgacaatta cttttttcat tccgcagcac tctattgaat cgaatgcaga aaaatctcta
     9181 cagatggttg aaaaagaagg aatgtatcct tctatcaatc aaggaaatat gctaggcact
     9241 cggttagata attttactga tcatttgatg atacgaaaaa caaaagcaga tcctgaattg
     9301 aactccttgg agaacgcgat gtctatggcg gattatcccc gttattggca tggttatcag
     9361 ctttttttac gacctttact gctagggatg agtttgggga gtattcgaat gatttacgca
     9421 gccattcttt ttctgctgat tggattgacg agctactttt taaccaagtg tagcgacatc
     9481 tatttaacga cagccttgtt gatttcgctt gctataggaa atgctgcaac ctttttcttc
     9541 agtatgcaat tttcaaatat ttggattttg actttattgt ctgtattact atttttaaca
     9601 aaaccacaac tctccaacac caaccaacaa ttattcttgt atttctttgt cattggttct
     9661 ttagctaact tttttgactt gttgacaaca ccggtcatct cttgggggat acctgtgatc
     9721 attatttatt atgtaacaaa taagtattta atggataaac gcagttcatt gagcaaacaa
     9781 gtaggcagtt ttgtttttac cggtatcttt tggggattag gctatggttt gacatgggtg
     9841 gctaaatgga tcctatcatc gatcatattg aacaaaaaca ttgtaaaaga tgcaatcaac
     9901 caaatcctct ttcgaacgga aggaaacgac aaatacccgt tgcatcgatt agagatgctg
     9961 aaaagcaata ttagattgat gtatccaaaa gtagcaatat tattgctatt gattacttgt
    10021 cttgtttttc tctattttgc ttatcgtaaa cgaaatagct ggcgtccgtt ccaacttcta
    10081 gttttgtttg ttttagctgc tgtaacgccg tatatttggt acaatatttt agctaatcac
    10141 tcacagattc attattggtt tacgtatcgt acgcaaatca tcacaagttt tgctatactt
    10201 agtgctttcg cctttttgat ccctcctgag aaatcaaaag acgaactaaa ttacttttaa
    10261 gaataccgat gtactaattc atttttaaac ataaaaactc cagctgtcag agatagccgg
    10321 agttttttag tatttttatt tcgccaaacg atagatggca tctgcataga ttgctgttgc
    10381 tctgaacaag tcatcaagac tcataaattc gtttgcttga tgcattgtat cagtatatcc
    10441 tgggaacatc gctccataag caacgccacg tttcaacaaa cgtccgtaag ttccgccacc
    10501 gatgatttgt tcgtagcctt tttctccagt gtggtcttca tagacttgca ataatgtagc
    10561 caccaatgga tcatccattg gtacgtaatg tggttccatt acgcgagcac ctcgagtaac
    10621 tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt tgcaatccgt ctactgttac
    10681 accttttgga aaacggaaat tcatattgat gaagttcgct tcctctgtac cattttcttc
    10741 aaaagcaaat agtccagcat tcattgtcaa tttgcccatt ttttcatctt cataagcgac
    10801 tcctagtttt tctccataaa aatcttcatg gacataagca gctgctacat ggataaattg
    10861 tttagcagat cctaggaatt catagttatc taagaacaat gccaagaaag aacctgcatt
    10921 gataccagat tgtgggcttg caccatgtgc gcctttacca actaattcga tttttacata
    10981 cgtattgtcc gcttcaatcg ttccgcttac tggatttgct tcgatgaatt ggtaaaatgc
    11041 ttcttccatc gcgatagctg catctgcact tggtacttga agtgctgcag ttgctgttcc
    11101 tggcaccata ttttcgcgaa gtccggaaac aaaagatttc aagacataat ctcctgcatt
    11161 gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt tcaccattga tgattgggaa
    11221 ttcagcatct ggtgagaaac caaaatctgg ttcttcttca tgttcgaaat aataagccat
    11281 atcgccccag ccgctttctt catcgctgcc gactacgaag cgaacttttt ttgatagttt
    11341 caagtcaagt tctttaatga tcttcatcgc atagtaagct gccatacttg gacctttgtc
    11401 atcgcttgat ccgcgtgcaa agatctttcc atccttgatt actggttcat aaggatctgt
    11461 gtcccagcca tctcctgcag gaacgacatc catgtgtcca aatatcccta gcgtttcgtc
    11521 tccttcgcca agatcaatat gccctgcata gttatcgaca ttttttactt taaatccatc
    11581 acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga ggtccaggtc caaatggtgc
    11641 atctggtgtt acttttgaat catcgcgctc gctgttgaca cgtaaaagat ttttcaagtc
    11701 ttcaagcaag tcttctttac gtgcttctac ttctttttgc caatcaattg tcataaagtt
    11761 aatcctccac ttatttaagt cttcgtgttc atgataccac aaattacaga aaaaaatagt
    11821 tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc taaaaaaaat atacgctata
    11881 atgagtaaaa ggaggttttt cgatgctgat aaacgaacaa ctagtgaaaa gagtcatcac
    11941 acctcgtcca aagatttcac ataaaggaac atttggacgt atcgttttga ttggcggcaa
    12001 tgaacaatat ggcggagcaa tcattatgag tgctttagct gccgtacaca gtggagcggg
    12061 tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc atctgccaga
    12121 agcaatgatt gtagattgga cggatactga ccagtttctt tccgtaataa agcaagcaga
    12181 tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac gtttcaaaca
    12241 agtactagaa aaacaatcaa aagaacaatg gctggtgatt gatggctctg ctttaagttt
    12301 acttgttgct aatcatcttt tgctacccta tcccgaacaa actgtactta cacctcatca
    12361 aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata aagaaaacaa
    12421 aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga caaaaattta
    12481 tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa caggcggcat
    12541 gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc aaaatgacca
    12601 aacgattgca gcagctgttt atcttcatag tttcattgga gaccagctgg caaaagaaca
    12661 atatattgtt ttgccgacac aaatcagcga agcacttccg aagtggatga attatttttg
    12721 taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt atgtgcgaga
    12781 acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt atatccgctg
    12841 gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata aaaaaatcga
    12901 agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag aaatgatcca
    12961 ttacggagaa aaagtgagca tccacccaac tatccaaaag tatacgggaa cacggttgga
    13021 ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg gcagtagtaa
    13081 acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag tcaatccgga
    13141 gttagaacag ttattccaag cgtacgcttc tttggatgat gtaaattctt aataaatacc
    13201 gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga aaaaagcagg
    13261 tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact ggctgtcgca
    13321 ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa tccgaagctt
    13381 ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca gattgattca
    13441 atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt gcagactgtg
    13501 tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat ctgcatagaa
    13561 aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct atacgtaaac
    13621 aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg ttagaagaac
    13681 aactagctat cgggcgtaca ccgattcgag aagcactgat ccaactgcgc aatcaagagc
    13741 ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc cgatctgctt
    13801 cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa tgcagtgcga
    13861 aaatgaccaa gcaagatcaa gctaaactag aaaaaatact agaacaaacc gatcaggcag
    13921 tgatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac cgaacttgtt
    13981 atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt acgcacttga
    14041 accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga gtattagatg
    14101 aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc ggatttcttt
    14161 gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat aattaccccg
    14221 attattttga agatattcag gacagaccca tcaaataaga ggttttggaa tcagcgtttt
    14281 gacctgagta ttagagaaat aaacggcaaa atagttcctc atattttgcc gttttttttg
    14341 agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg aaaaagctgc
    14401 cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt taacttggct
    14461 gttcttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc gtgagcctgc
    14521 cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    14581 ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa aaaaggactg
    14641 tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc gtacatcttt
    14701 tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta tatggccaaa
    14761 gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct tttgaaaaac
    14821 tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa aaaggcaacc
    14881 gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct tcgatgtggt
    14941 gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc gaagctagtc
    15001 ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca tcttctgcat
    15061 aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg attggaacag
    15121 ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg atgctacatg
    15181 ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg gacaaaggaa
    15241 tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct tcggggatct
    15301 gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc ggcaacgacg
    15361 atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag ctgtagttgt
    15421 tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata aaattctggt
    15481 cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc gacagctgtc
    15541 atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac cttgataaat
    15601 cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg ttcgccgcat
    15661 acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa tcgcatatcc
    15721 tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc ggttgctttc
    15781 acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc gatttctacg
    15841 tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat cgcttcattt
    15901 ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac aacatctcgg
    15961 accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg aatcgaatat
    16021 ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc ccctacacct
    16081 acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa aatcaccggt
    16141 tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac cgtcgtatga
    16201 caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc ataagaaaaa
    16261 tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat agtcacttct
    16321 tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt aggagagaaa
    16381 aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata atctagaccg
    16441 gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc ccgtaccatc
    16501 gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt tgggtggcca
    16561 cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat attggttgtt
    16621 gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa agagcccatg
    16681 actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat ttcattgatt
    16741 tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc tgcttctcgt
    16801 acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga tgcattgatt
    16861 gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga agccatcgtt
    16921 gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc cccattatct
    16981 actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa agaagaaatc
    17041 gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt gacaatctct
    17101 atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt tgctaaaaaa
    17161 ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc tccttcaatc
    17221 agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc tggactgata
    17281 ttattttccc gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac tccacggaca
    17341 tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc attatcgatt
    17401 gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa acgtttcatt
    17461 ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag gcgttcccgt
    17521 acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt gggatggatt
    17581 tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca acccaagatt
    17641 gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg aacaaaaagg
    17701 ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag cctgaaccga
    17761 ctagattcat tggaccgcag aaaaatccta gtggtgtcaa tatagcaaat acgattgcca
    17821 agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa ggagaagcat
    17881 acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt ggtgcaacat
    17941 ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta taacctccgc
    18001 gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct aagatgattg
    18061 gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat gagatcattg
    18121 gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc tttttgttca
    18181 ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca atcataccaa
    18241 tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca gcataaatcg
    18301 tttgatattg tttgaaattg acgatattag caaagattcc agccataatc gagcccataa
    18361 atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc aacacaataa
    18421 ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca gcggttacaa
    18481 tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact acctttcgaa
    18541 tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca ccaaagaaga
    18601 catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata tgcgtcaaca
    18661 catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt ccgactagcg
    18721 ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg tagcctttta
    18781 cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata ataatatcac
    18841 tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata tcagtcaaac
    18901 tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg ttcagttttc
    18961 aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg tttcatttga
    19021 tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct tctttcctct
    19081 ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca atgcagtaaa
    19141 tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt cgatttcagc
    19201 aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag ctgctgcgtt
    19261 gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat cgcgatatgc
    19321 gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt gttttgaacc
    19381 gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta ctgttccacc
    19441 tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat tctgttattt
    19501 gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg ccactagaaa
    19561 ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg aaacggtgac
    19621 ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa cgttttgctg
    19681 atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat ctttcgttat
    19741 ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt cgtttttcgg
    19801 tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca ctgttgcaat
    19861 atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt tatgggtaat
    19921 ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca agaacttacg
    19981 tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta agcgaacttc
    20041 ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt gttgttcgaa
    20101 acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag gggcaactaa
    20161 cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg tcttaactcc
    20221 taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc cgtcgttaac
    20281 catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag aaaagcttac
    20341 cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac ttcacctaat
    20401 agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga agcgggtgct
    20461 tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg tcaatgtttt
    20521 tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc tagcagtgat
    20581 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
    20641 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
    20701 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
    20761 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
    20821 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
    20881 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
    20941 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
    21001 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
    21061 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
    21121 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
    21181 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
    21241 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
    21301 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
    21361 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
    21421 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
    21481 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
    21541 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
    21601 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
    21661 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
    21721 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
    21781 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
    21841 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
    21901 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
    21961 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
    22021 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
    22081 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
    22141 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
    22201 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
    22261 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
    22321 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
    22381 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
    22441 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
    22501 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
    22561 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
    22621 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
    22681 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
    22741 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
    22801 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
    22861 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
    22921 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
    22981 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
    23041 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
    23101 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
    23161 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
    23221 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
    23281 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
    23341 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
    23401 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
    23461 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
    23521 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
    23581 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
    23641 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
    23701 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
    23761 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
    23821 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
    23881 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
    23941 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
    24001 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
    24061 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
    24121 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
    24181 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
    24241 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
    24301 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
    24361 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
    24421 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
    24481 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
    24541 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
    24601 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
    24661 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
    24721 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
    24781 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
    24841 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
    24901 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
    24961 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
    25021 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
    25081 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
    25141 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
    25201 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
    25261 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
    25321 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
    25381 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
    25441 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
    25501 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
    25561 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
    25621 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
    25681 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
    25741 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
    25801 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
    25861 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
    25921 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
    25981 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
    26041 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
    26101 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
    26161 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
    26221 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
    26281 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
    26341 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
    26401 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
    26461 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
    26521 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
    26581 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
    26641 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
    26701 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
    26761 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
    26821 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
    26881 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
    26941 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
    27001 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
    27061 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
    27121 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
    27181 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
    27241 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
    27301 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
    27361 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
    27421 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
    27481 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
    27541 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
    27601 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
    27661 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
    27721 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
    27781 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
    27841 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
    27901 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
    27961 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
    28021 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
    28081 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
    28141 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
    28201 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
    28261 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
    28321 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
    28381 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
    28441 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
    28501 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
    28561 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
    28621 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
    28681 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
    28741 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
    28801 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
    28861 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
    28921 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
    28981 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
    29041 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
    29101 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
    29161 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
    29221 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
    29281 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
    29341 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
    29401 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
    29461 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
    29521 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
    29581 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
    29641 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
    29701 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
    29761 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
    29821 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
    29881 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
    29941 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
    30001 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
    30061 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
    30121 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
    30181 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
    30241 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
    30301 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
    30361 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
    30421 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
    30481 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
    30541 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    30601 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    30661 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    30721 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    30781 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    30841 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    30901 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    30961 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    31021 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    31081 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    31141 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    31201 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    31261 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    31321 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    31381 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    31441 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    31501 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    31561 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    31621 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    31681 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    31741 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    31801 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    31861 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    31921 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    31981 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    32041 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    32101 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    32161 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    32221 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    32281 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga aacttccaaa
    32341 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    32401 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    32461 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    32521 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    32581 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    32641 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    32701 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    32761 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    32821 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    32881 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    32941 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    33001 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    33061 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    33121 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    33181 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    33241 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    33301 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    33361 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    33421 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    33481 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    33541 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    33601 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    33661 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    33721 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    33781 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    33841 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    33901 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    33961 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    34021 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    34081 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    34141 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    34201 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    34261 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    34321 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    34381 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    34441 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    34501 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    34561 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    34621 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    34681 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    34741 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    34801 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    34861 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    34921 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    34981 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    35041 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    35101 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    35161 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    35221 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    35281 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    35341 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    35401 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    35461 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    35521 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    35581 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    35641 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    35701 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    35761 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    35821 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    35881 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    35941 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    36001 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    36061 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    36121 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    36181 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    36241 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    36301 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    36361 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    36421 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    36481 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    36541 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    36601 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    36661 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    36721 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    36781 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    36841 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaacggtgta cttcttccaa
    36901 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    36961 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    37021 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    37081 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    37141 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    37201 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    37261 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    37321 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    37381 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    37441 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    37501 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    37561 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    37621 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    37681 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    37741 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    37801 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    37861 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    37921 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    37981 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    38041 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    38101 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    38161 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    38221 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    38281 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    38341 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    38401 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    38461 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    38521 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    38581 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    38641 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    38701 aacaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    38761 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    38821 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    38881 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    38941 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    39001 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    39061 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    39121 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    39181 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    39241 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    39301 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    39361 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    39421 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    39481 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    39541 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    39601 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    39661 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    39721 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    39781 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    39841 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtgtttc
    39901 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    39961 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    40021 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    40081 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    40141 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    40201 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    40261 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    40321 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    40381 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    40441 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    40501 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    40561 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    40621 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    40681 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    40741 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    40801 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    40861 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    40921 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    40981 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    41041 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    41101 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    41161 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    41221 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    41281 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    41341 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    41401 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    41461 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    41521 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    41581 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    41641 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    41701 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    41761 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    41821 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    41881 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    41941 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    42001 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    42061 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    42121 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    42181 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    42241 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    42301 catcacacgg atcttctttc cctccaaatc ttccggcgta tggatcggac cgtctaccat
    42361 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    42421 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    42481 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    42541 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    42601 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    42661 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    42721 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    42781 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    42841 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    42901 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    42961 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    43021 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    43081 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    43141 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    43201 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    43261 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    43321 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    43381 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    43441 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    43501 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    43561 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    43621 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    43681 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    43741 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    43801 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    43861 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    43921 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    43981 tctttacgcc acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    44041 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    44101 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc
    44161 aagttctttt tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt
    44221 aagcatcaat gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg
    44281 tacattggtc ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt
    44341 gtgtgactcc ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa
    44401 cagccgttgc taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt
    44461 ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat
    44521 gtttttgtgg atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc
    44581 gtccatgagg gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta
    44641 ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg
    44701 attccatatc aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt
    44761 gtaggctcaa tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat
    44821 gtgttaacac atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt
    44881 gaggatcgac gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat
    44941 gtgtttcact gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg
    45001 tcgggataca tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga
    45061 tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc
    45121 acattccctt agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct
    45181 tgaagtctgc catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa
    45241 catctgagaa gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg
    45301 tatcgcagta accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt
    45361 tttccatttt ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt
    45421 tgaaccattg catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag
    45481 cagagacgtt acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg
    45541 gaatcatttc gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa
    45601 tacgacaagc tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga
    45661 taactgctgt atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat
    45721 ggtccgtgtt gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg
    45781 gataatctgg gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac
    45841 tgattcctgc caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg
    45901 catattttcc ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga
    45961 tataaacttg gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga
    46021 tcacagcttg ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat
    46081 cgttcactgc acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac
    46141 ctacacctaa agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg
    46201 tcgctaagac gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag
    46261 gatgttcgat ccattggata caattttcag gagctccggc agcgatagca gcatcacgta
    46321 ccactcgtgc tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg
    46381 gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg
    46441 ttggagttac tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga
    46501 catcttcatt gatcacacca actgttttat catgtttgat attatgccaa atatattcgg
    46561 acgcatacat atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag
    46621 ccatcttcgc taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat
    46681 ggtctacttt ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg
    46741 ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt
    46801 tagccatcat tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc
    46861 atacaatatt tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta
    46921 aatataaaat tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta
    46981 ttatttatat gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc
    47041 tttctttttt gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata
    47101 agctttgata cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca
    47161 gtttttactt caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca
    47221 tttgttacga cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag
    47281 atcccttcgc agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca
    47341 tgtaatccgc cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga
    47401 cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg
    47461 ataaacatca caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta
    47521 caaaaaacac tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag
    47581 attttccttt agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct
    47641 tctctaaatt caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc
    47701 aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg
    47761 atcaaatggc ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca
    47821 tcaattggac ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa
    47881 gtcatacatg tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact
    47941 ccacggataa caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt
    48001 ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt
    48061 tccccgacag ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc
    48121 gcgctttgac gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga
    48181 cggtctggat tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact
    48241 gattttttca cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg
    48301 atatcagagc ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc
    48361 gaaccgttca agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt
    48421 aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat
    48481 ttatgcagtc cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat
    48541 gtattactta aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt
    48601 actgtagcta atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg
    48661 atcaattccc ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg
    48721 gcaggttgcg tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat
    48781 cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat
    48841 tctttttttt atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata
    48901 ttttttgata catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct
    48961 tctgcagaat taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt
    49021 ttactaaatc taatccctac attgattggt attgctacta ttcttcttat tatgattcct
    49081 attatcttgt ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact
    49141 tcaggaggcg gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg
    49201 ttcatgagcg gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc
    49261 gaaccattca aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc
    49321 ttagctgtgc tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga
    49381 atcattaaaa gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa
    49441 acaggacaaa cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt
    49501 tataaaggac aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta
    49561 gcaactgctg gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca
    49621 ttttatgaac aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg
    49681 gcaggagaac accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag
    49741 gaggtgtgac aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc
    49801 aacttagaaa cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc
    49861 agttcattca ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa
    49921 ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta
    49981 atagctaatg ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg
    50041 aagatcgatt ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc
    50101 aagaatgcaa cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg
    50161 acataactac ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg
    50221 gcaatttttt gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga
    50281 tccatgatgc cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca
    50341 actacattga caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc
    50401 gcaatcaaaa caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta
    50461 cgaatattct gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg
    50521 tcatccgtca aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg
    50581 gcacacccaa cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc
    50641 gctacgactg cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt
    50701 acccgagcat agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga
    50761 tgcagttctg atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct
    50821 atggcactgg cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt
    50881 ttcgctcgct tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga
    50941 ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca
    51001 taagtagcat aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg
    51061 atcgccgctt gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct
    51121 ttggtgattg agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca
    51181 gaatcaaaag gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga
    51241 taattctctt caacagccct tacgatggct acttctgttg gattaccttt gataacaagg
    51301 tcgtcatctt cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc
    51361 atcgcgctgt tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc
    51421 cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta
    51481 gcggttccta gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc
    51541 ttctgcacac cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct
    51601 gcaacagcca aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca
    51661 ccaatcacaa atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc
    51721 tgattcaatc ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg
    51781 atcttaccca tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca
    51841 gcaaccgtac atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca
    51901 gcaataaatg ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct
    51961 acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct
    52021 aaaacaagaa tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct
    52081 cgtatgacag ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg
    52141 tttccttctt ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt
    52201 ccttcaaata aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct
    52261 acaagaagga tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat
    52321 tctttcggcg cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct
    52381 tgtgaagaca acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc
    52441 tgcttgtatt cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat
    52501 tttactataa catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa
    52561 gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata
    52621 gatgtaagga aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa
    52681 gcaaacaaag ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta
    52741 taaatagtga atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg
    52801 atacgtaaca attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca
    52861 aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg
    52921 ctttgcatgt atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac
    52981 aagaaagccg agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct
    53041 tttttgttat tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca
    53101 aaactaaaga aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct
    53161 cttcctgcaa aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt
    53221 gagaaagctt ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg
    53281 ctgtctgctt tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa
    53341 gttgttccga cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc
    53401 gcttcttccg tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg
    53461 ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc
    53521 tttgcttcga tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca
    53581 gcagaaccat tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct
    53641 ttgatatatg gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct
    53701 tcatactcaa agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc
    53761 aatctgccat caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc
    53821 aatgtctccc acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca
    53881 gtatctggtt tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc
    53941 gcatcaccag gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca
    54001 gttttccgat ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg
    54061 attaattctt ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact
    54121 ttctatcatg tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt
    54181 tttcacttct attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt
    54241 gttttaggca tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca
    54301 tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg
    54361 tcaatctttc atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa
    54421 aaacgacgac gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga
    54481 ttgatggaaa atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg
    54541 ttatccaaag tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc
    54601 aaaaattgtt gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc
    54661 ttgtggaatt gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc
    54721 agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg
    54781 agccattcgg tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt
    54841 tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta
    54901 aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag
    54961 aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc
    55021 tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca
    55081 cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag
    55141 atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt
    55201 tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt
    55261 acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga
    55321 aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat
    55381 tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag
    55441 agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc
    55501 acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga
    55561 tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg
    55621 ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg
    55681 cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt
    55741 attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt
    55801 atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat
    55861 tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg
    55921 catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg
    55981 gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag
    56041 cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta
    56101 gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct
    56161 tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc
    56221 gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt
    56281 tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa
    56341 ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga
    56401 tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt
    56461 gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctatg gttttgaaaa
    56521 tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag
    56581 cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg
    56641 taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt
    56701 acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct
    56761 tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg
    56821 tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac
    56881 gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca
    56941 ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt
    57001 cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt
    57061 caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt
    57121 cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg
    57181 atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc
    57241 cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc
    57301 aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat
    57361 accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact
    57421 gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc
    57481 ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct
    57541 ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt
    57601 ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt
    57661 ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa
    57721 aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca
    57781 ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat
    57841 ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa
    57901 gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa
    57961 ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct
    58021 tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc
    58081 cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata
    58141 caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct
    58201 cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc
    58261 gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg
    58321 atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc
    58381 attgccgttg atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg
    58441 agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact
    58501 ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa
    58561 ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc
    58621 tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc
    58681 cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc
    58741 tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa
    58801 aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt
    58861 agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg
    58921 ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc
    58981 tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca
    59041 tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc
    59101 tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact
    59161 ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat
    59221 catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac
    59281 ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata
    59341 cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct
    59401 ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca
    59461 ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa
    59521 tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc
    59581 ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc
    59641 aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt
    59701 gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata
    59761 tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg
    59821 gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt
    59881 tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg
    59941 cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag
    60001 acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat
    60061 tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct
    60121 aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc
    60181 acctttagct gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc
    60241 tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg
    60301 atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata
    60361 ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt
    60421 ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga
    60481 taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg
    60541 atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca
    60601 ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat
    60661 acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt
    60721 tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata
    60781 atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca
    60841 tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct
    60901 tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata
    60961 gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa
    61021 cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac
    61081 atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa
    61141 ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa
    61201 ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga
    61261 acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat
    61321 gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca
    61381 gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa
    61441 atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc
    61501 acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata
    61561 aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc
    61621 atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc
    61681 accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag
    61741 tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg
    61801 acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt
    61861 aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca
    61921 gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac
    61981 gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca
    62041 gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct
    62101 ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata
    62161 gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa
    62221 aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg
    62281 ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc
    62341 ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt
    62401 actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc
    62461 atctgcggta ataagctcaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt
    62521 cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc
    62581 tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt
    62641 tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg
    62701 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
    62761 gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca
    62821 tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
    62881 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
    62941 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
    63001 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
    63061 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
    63121 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
    63181 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
    63241 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
    63301 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
    63361 tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct
    63421 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
    63481 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
    63541 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
    63601 aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct
    63661 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
    63721 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
    63781 gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct
    63841 ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg
    63901 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
    63961 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
    64021 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
    64081 tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
    64141 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
    64201 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
    64261 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca
    64321 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
    64381 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt
    64441 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
    64501 tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct
    64561 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
    64621 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat
    64681 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
    64741 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta
    64801 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
    64861 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
    64921 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
    64981 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
    65041 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
    65101 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
    65161 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
    65221 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
    65281 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
    65341 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
    65401 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
    65461 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
    65521 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
    65581 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
    65641 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
    65701 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
    65761 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
    65821 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
    65881 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
    65941 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
    66001 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
    66061 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
    66121 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
    66181 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
    66241 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
    66301 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
    66361 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
    66421 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
    66481 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
    66541 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
    66601 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
    66661 ttctttaagt ttacgtttat gtctttattt tgacaaaaaa atgtcttttc gttttggcgc
    66721 atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa
    66781 gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa
    66841 aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta
    66901 cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct
    66961 cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt
    67021 aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga
    67081 gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg
    67141 accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact
    67201 agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat
    67261 ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt
    67321 tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca
    67381 atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc
    67441 ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg
    67501 ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca
    67561 aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa
    67621 tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt
    67681 cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata
    67741 gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct
    67801 tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc
    67861 ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc
    67921 ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct
    67981 tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc
    68041 aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca
    68101 taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca
    68161 tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc
    68221 agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc
    68281 cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta
    68341 caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct
    68401 agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt
    68461 actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata
    68521 aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca
    68581 caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg
    68641 tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca
    68701 tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa
    68761 gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt
    68821 tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca
    68881 ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca
    68941 gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact
    69001 tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca
    69061 atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc
    69121 ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac
    69181 caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt
    69241 aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt
    69301 tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg
    69361 ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca
    69421 ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg
    69481 atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact
    69541 aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga
    69601 ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat
    69661 gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc
    69721 acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg
    69781 gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag
    69841 ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca
    69901 aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac
    69961 aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg
    70021 tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac
    70081 aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc
    70141 aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta
    70201 acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga
    70261 tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca
    70321 tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg
    70381 tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg
    70441 atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc
    70501 cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact
    70561 aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca
    70621 gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt
    70681 ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc
    70741 aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt
    70801 taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt
    70861 ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa
    70921 aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat
    70981 tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc
    71041 atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt
    71101 tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt
    71161 agcataatac tatctgttgc tgcaagattt tgtttgatag catcaatcaa tttattcttt
    71221 tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt
    71281 gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca
    71341 ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca
    71401 gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct
    71461 gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat
    71521 tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac
    71581 agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca
    71641 ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt
    71701 gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact
    71761 aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct
    71821 gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga
    71881 ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt
    71941 gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta
    72001 tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc
    72061 gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt
    72121 ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt
    72181 gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga
    72241 gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg
    72301 ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc
    72361 ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg
    72421 gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa
    72481 aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta
    72541 ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt
    72601 ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact
    72661 ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt
    72721 acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt
    72781 tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta
    72841 acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca
    72901 tttcgatttt attattgata tattttgctt cataatcata gaatgctacg tcttttacga
    72961 cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc
    73021 gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag
    73081 cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg
    73141 ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag
    73201 gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag
    73261 cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg
    73321 cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt
    73381 ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga
    73441 tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga
    73501 catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg
    73561 taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg
    73621 cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca
    73681 aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa
    73741 acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg
    73801 tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag
    73861 aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg
    73921 agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt
    73981 aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt
    74041 tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt
    74101 acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt
    74161 atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc
    74221 tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat
    74281 cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata
    74341 cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt
    74401 gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa
    74461 caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt
    74521 gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa
    74581 aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct
    74641 tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca
    74701 tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa
    74761 aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc
    74821 agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct
    74881 ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc
    74941 ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag
    75001 tcggttccac caagaactga caagttttct tgaacagtat tatgattctc ctattgatga
    75061 aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca
    75121 gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg
    75181 cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt
    75241 tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg
    75301 gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc
    75361 gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga
    75421 aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc
    75481 cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt
    75541 tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat
    75601 gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata
    75661 agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga
    75721 agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta
    75781 ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt
    75841 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    75901 cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg
    75961 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    76021 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca
    76081 cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    76141 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    76201 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    76261 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    76321 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    76381 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    76441 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    76501 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    76561 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa
    76621 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    76681 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    76741 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    76801 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt
    76861 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    76921 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    76981 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    77041 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    77101 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    77161 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    77221 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    77281 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    77341 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    77401 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    77461 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    77521 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    77581 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    77641 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    77701 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    77761 taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc
    77821 tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct
    77881 tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc
    77941 aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt
    78001 taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat
    78061 ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc
    78121 tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc
    78181 gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta
    78241 tttagcaaat ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa
    78301 ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt
    78361 tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg
    78421 atcggcacaa aagcttccgg ccatacttct tgtatgactg ccatcagctc atccagttca
    78481 ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct
    78541 tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga ccatggtttc
    78601 aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga
    78661 cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata
    78721 tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata
    78781 agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc
    78841 actaggccaa tacaactata taaaagccca aatccattgc caaacatcag catgcccgct
    78901 actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca
    78961 aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat
    79021 cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata
    79081 atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc
    79141 cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DACIWG010000003        78696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.003, whole genome shotgun sequence.
ACCESSION   DACIWG010000003 DACIWG010000000
VERSION     DACIWG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.003"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            194..2101
                     /locus_tag="IYF54_000160"
     CDS             194..2101
                     /locus_tag="IYF54_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ9830532.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2642..5920)
                     /locus_tag="IYF54_000161"
     CDS             complement(2642..5920)
                     /locus_tag="IYF54_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ9830533.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6191..6736)
                     /locus_tag="IYF54_000162"
     CDS             complement(6191..6736)
                     /locus_tag="IYF54_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9830534.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(6748..7440)
                     /locus_tag="IYF54_000163"
     CDS             complement(6748..7440)
                     /locus_tag="IYF54_000163"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ9830535.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(7459..7743)
                     /locus_tag="IYF54_000164"
     CDS             complement(7459..7743)
                     /locus_tag="IYF54_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830536.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(7756..8319)
                     /locus_tag="IYF54_000165"
     CDS             complement(7756..8319)
                     /locus_tag="IYF54_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ9830537.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            8547..9209
                     /locus_tag="IYF54_000166"
     CDS             8547..9209
                     /locus_tag="IYF54_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ9830538.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            9333..10529
                     /locus_tag="IYF54_000167"
     CDS             9333..10529
                     /locus_tag="IYF54_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ9830539.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(10630..12006)
                     /locus_tag="IYF54_000168"
     CDS             complement(10630..12006)
                     /locus_tag="IYF54_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830540.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(12043..12909)
                     /locus_tag="IYF54_000169"
     CDS             complement(12043..12909)
                     /locus_tag="IYF54_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ9830541.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(12998..14023)
                     /locus_tag="IYF54_000170"
     CDS             complement(12998..14023)
                     /locus_tag="IYF54_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9830542.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(14041..14715)
                     /locus_tag="IYF54_000171"
     CDS             complement(14041..14715)
                     /locus_tag="IYF54_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9830543.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(14899..15792)
                     /locus_tag="IYF54_000172"
     CDS             complement(14899..15792)
                     /locus_tag="IYF54_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9830544.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(15866..17563)
                     /locus_tag="IYF54_000173"
     CDS             complement(15866..17563)
                     /locus_tag="IYF54_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9830545.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(17556..18869)
                     /locus_tag="IYF54_000174"
     CDS             complement(17556..18869)
                     /locus_tag="IYF54_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9830546.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(18869..22906)
                     /locus_tag="IYF54_000175"
     CDS             complement(18869..22906)
                     /locus_tag="IYF54_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ9830547.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(22919..23377)
                     /locus_tag="IYF54_000176"
     CDS             complement(22919..23377)
                     /locus_tag="IYF54_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9830548.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(23675..24193)
                     /locus_tag="IYF54_000177"
     CDS             complement(23675..24193)
                     /locus_tag="IYF54_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ9830549.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(24195..24812)
                     /locus_tag="IYF54_000178"
     CDS             complement(24195..24812)
                     /locus_tag="IYF54_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ9830550.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(24809..25663)
                     /locus_tag="IYF54_000179"
     CDS             complement(24809..25663)
                     /locus_tag="IYF54_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9830551.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(25660..26571)
                     /locus_tag="IYF54_000180"
     CDS             complement(25660..26571)
                     /locus_tag="IYF54_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9830552.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(26612..27946)
                     /locus_tag="IYF54_000181"
     CDS             complement(26612..27946)
                     /locus_tag="IYF54_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9830553.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            28154..28984
                     /locus_tag="IYF54_000182"
     CDS             28154..28984
                     /locus_tag="IYF54_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ9830554.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(29228..29477)
                     /locus_tag="IYF54_000183"
                     /pseudo
     CDS             complement(29228..29477)
                     /locus_tag="IYF54_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29668..29862)
                     /locus_tag="IYF54_000184"
     CDS             complement(29668..29862)
                     /locus_tag="IYF54_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830555.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(30367..30552)
                     /locus_tag="IYF54_000185"
     CDS             complement(30367..30552)
                     /locus_tag="IYF54_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830556.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(31402..31743)
                     /locus_tag="IYF54_000186"
     CDS             complement(31402..31743)
                     /locus_tag="IYF54_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ9830557.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(31743..31973)
                     /locus_tag="IYF54_000187"
     CDS             complement(31743..31973)
                     /locus_tag="IYF54_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9830558.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            32260..32598
                     /locus_tag="IYF54_000188"
     CDS             32260..32598
                     /locus_tag="IYF54_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830559.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(32803..33042)
                     /locus_tag="IYF54_000189"
     CDS             complement(32803..33042)
                     /locus_tag="IYF54_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ9830560.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(33018..33365)
                     /locus_tag="IYF54_000190"
     CDS             complement(33018..33365)
                     /locus_tag="IYF54_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ9830561.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(34097..34678)
                     /locus_tag="IYF54_000191"
     CDS             complement(34097..34678)
                     /locus_tag="IYF54_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9830562.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(34861..35487)
                     /locus_tag="IYF54_000192"
     CDS             complement(34861..35487)
                     /locus_tag="IYF54_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9830563.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(35509..36840)
                     /locus_tag="IYF54_000193"
     CDS             complement(35509..36840)
                     /locus_tag="IYF54_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ9830564.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(36995..37396)
                     /locus_tag="IYF54_000194"
     CDS             complement(36995..37396)
                     /locus_tag="IYF54_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ9830565.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(37482..37688)
                     /locus_tag="IYF54_000195"
     CDS             complement(37482..37688)
                     /locus_tag="IYF54_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830566.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(37783..39285)
                     /locus_tag="IYF54_000196"
     CDS             complement(37783..39285)
                     /locus_tag="IYF54_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ9830567.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(39303..39758)
                     /gene="argR"
                     /locus_tag="IYF54_000197"
     CDS             complement(39303..39758)
                     /gene="argR"
                     /locus_tag="IYF54_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ9830568.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(39856..40473)
                     /locus_tag="IYF54_000198"
     CDS             complement(39856..40473)
                     /locus_tag="IYF54_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830569.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            40694..42067
                     /locus_tag="IYF54_000199"
     CDS             40694..42067
                     /locus_tag="IYF54_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9830570.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            42142..43254
                     /locus_tag="IYF54_000200"
     CDS             42142..43254
                     /locus_tag="IYF54_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ9830571.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(43326..44963)
                     /locus_tag="IYF54_000201"
     CDS             complement(43326..44963)
                     /locus_tag="IYF54_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ9830572.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(45131..45739)
                     /locus_tag="IYF54_000202"
     CDS             complement(45131..45739)
                     /locus_tag="IYF54_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9830573.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(46123..47274)
                     /gene="queG"
                     /locus_tag="IYF54_000203"
     CDS             complement(46123..47274)
                     /gene="queG"
                     /locus_tag="IYF54_000203"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ9830574.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(47293..48045)
                     /locus_tag="IYF54_000204"
     CDS             complement(47293..48045)
                     /locus_tag="IYF54_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830575.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(48186..49136)
                     /locus_tag="IYF54_000205"
     CDS             complement(48186..49136)
                     /locus_tag="IYF54_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ9830576.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(49137..49976)
                     /locus_tag="IYF54_000206"
     CDS             complement(49137..49976)
                     /locus_tag="IYF54_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ9830577.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(49973..50719)
                     /locus_tag="IYF54_000207"
     CDS             complement(49973..50719)
                     /locus_tag="IYF54_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830578.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            50957..52327
                     /locus_tag="IYF54_000208"
     CDS             50957..52327
                     /locus_tag="IYF54_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830579.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(52382..53278)
                     /locus_tag="IYF54_000209"
     CDS             complement(52382..53278)
                     /locus_tag="IYF54_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9830580.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(53559..54236)
                     /locus_tag="IYF54_000210"
     CDS             complement(53559..54236)
                     /locus_tag="IYF54_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9830581.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(54327..55232)
                     /locus_tag="IYF54_000211"
     CDS             complement(54327..55232)
                     /locus_tag="IYF54_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9830582.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            55407..56861
                     /locus_tag="IYF54_000212"
     CDS             55407..56861
                     /locus_tag="IYF54_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9830583.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            56877..57908
                     /locus_tag="IYF54_000213"
     CDS             56877..57908
                     /locus_tag="IYF54_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9830584.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            57915..59102
                     /locus_tag="IYF54_000214"
     CDS             57915..59102
                     /locus_tag="IYF54_000214"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ9830585.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            59258..60640
                     /locus_tag="IYF54_000215"
     CDS             59258..60640
                     /locus_tag="IYF54_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ9830586.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            60767..60997
                     /locus_tag="IYF54_000216"
     CDS             60767..60997
                     /locus_tag="IYF54_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830587.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(61197..61865)
                     /locus_tag="IYF54_000217"
     CDS             complement(61197..61865)
                     /locus_tag="IYF54_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ9830588.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(62054..62851)
                     /locus_tag="IYF54_000218"
                     /pseudo
     CDS             complement(62054..62851)
                     /locus_tag="IYF54_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(63132..64511)
                     /locus_tag="IYF54_000219"
     CDS             complement(63132..64511)
                     /locus_tag="IYF54_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9830589.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(64686..65855)
                     /locus_tag="IYF54_000220"
     CDS             complement(64686..65855)
                     /locus_tag="IYF54_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9830590.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(65830..66981)
                     /locus_tag="IYF54_000221"
     CDS             complement(65830..66981)
                     /locus_tag="IYF54_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ9830591.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(66941..68302)
                     /locus_tag="IYF54_000222"
     CDS             complement(66941..68302)
                     /locus_tag="IYF54_000222"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ9830592.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(68299..69300)
                     /locus_tag="IYF54_000223"
     CDS             complement(68299..69300)
                     /locus_tag="IYF54_000223"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ9830593.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(69316..70011)
                     /gene="dapD"
                     /locus_tag="IYF54_000224"
     CDS             complement(69316..70011)
                     /gene="dapD"
                     /locus_tag="IYF54_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ9830594.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(70017..70733)
                     /locus_tag="IYF54_000225"
     CDS             complement(70017..70733)
                     /locus_tag="IYF54_000225"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ9830595.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(70730..71611)
                     /locus_tag="IYF54_000226"
     CDS             complement(70730..71611)
                     /locus_tag="IYF54_000226"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ9830596.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(71614..72414)
                     /gene="dapF"
                     /locus_tag="IYF54_000227"
     CDS             complement(71614..72414)
                     /gene="dapF"
                     /locus_tag="IYF54_000227"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ9830597.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(72401..73660)
                     /gene="lysA"
                     /locus_tag="IYF54_000228"
     CDS             complement(72401..73660)
                     /gene="lysA"
                     /locus_tag="IYF54_000228"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ9830598.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(74146..74709)
                     /gene="efp"
                     /locus_tag="IYF54_000229"
     CDS             complement(74146..74709)
                     /gene="efp"
                     /locus_tag="IYF54_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ9830599.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            75137..76054
                     /locus_tag="IYF54_000230"
     CDS             75137..76054
                     /locus_tag="IYF54_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ9830600.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            76067..77212
                     /locus_tag="IYF54_000231"
     CDS             76067..77212
                     /locus_tag="IYF54_000231"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ9830601.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            77383..77841
                     /locus_tag="IYF54_000232"
     CDS             77383..77841
                     /locus_tag="IYF54_000232"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ9830602.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            78000..78470
                     /locus_tag="IYF54_000233"
     CDS             78000..78470
                     /locus_tag="IYF54_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ9830603.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(78523..78638)
                     /gene="rrf"
                     /locus_tag="IYF54_000234"
     rRNA            complement(78523..78638)
                     /gene="rrf"
                     /locus_tag="IYF54_000234"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        22586 a        16470 c        14000 g        25640 t
ORIGIN      
        1 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
       61 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
      121 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
      181 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
      241 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
      301 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
      361 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
      421 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
      481 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
      541 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
      601 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
      661 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
      721 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
      781 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
      841 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
      901 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
      961 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
     1021 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     1081 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     1141 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     1201 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     1261 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     1321 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     1381 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     1441 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     1501 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     1561 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     1621 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     1681 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     1741 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     1801 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     1861 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     1921 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     1981 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     2041 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     2101 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     2161 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     2221 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     2281 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     2341 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     2401 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     2461 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     2521 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     2581 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     2641 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     2701 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     2761 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     2821 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     2881 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     2941 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     3001 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     3061 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     3121 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     3181 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     3241 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     3301 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     3361 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     3421 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     3481 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     3541 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     3601 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     3661 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     3721 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     3781 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     3841 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     3901 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     3961 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     4021 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     4081 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     4141 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     4201 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     4261 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     4321 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     4381 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     4441 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     4501 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     4561 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     4621 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     4681 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     4741 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     4801 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     4861 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     4921 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     4981 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     5041 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     5101 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     5161 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     5221 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     5281 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     5341 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     5401 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     5461 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     5521 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     5581 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     5641 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     5701 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     5761 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     5821 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     5881 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     5941 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     6001 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
     6061 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
     6121 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
     6181 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
     6241 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
     6301 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
     6361 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
     6421 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
     6481 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
     6541 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
     6601 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
     6661 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
     6721 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
     6781 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
     6841 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
     6901 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
     6961 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
     7021 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
     7081 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
     7141 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     7201 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     7261 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     7321 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     7381 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     7441 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     7501 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     7561 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     7621 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     7681 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     7741 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     7801 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     7861 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     7921 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     7981 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     8041 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     8101 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     8161 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
     8221 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     8281 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     8341 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     8401 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     8461 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     8521 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     8581 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     8641 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     8701 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     8761 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     8821 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     8881 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     8941 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     9001 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     9061 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     9121 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     9181 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     9241 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     9301 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     9361 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     9421 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     9481 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     9541 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     9601 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     9661 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     9721 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     9781 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     9841 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     9901 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     9961 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
    10021 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
    10081 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
    10141 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
    10201 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
    10261 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
    10321 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
    10381 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
    10441 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
    10501 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
    10561 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
    10621 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
    10681 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
    10741 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
    10801 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
    10861 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
    10921 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
    10981 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
    11041 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
    11101 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
    11161 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
    11221 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
    11281 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
    11341 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
    11401 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
    11461 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
    11521 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
    11581 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
    11641 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
    11701 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
    11761 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
    11821 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
    11881 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
    11941 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
    12001 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
    12061 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
    12121 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
    12181 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
    12241 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
    12301 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
    12361 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
    12421 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
    12481 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
    12541 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
    12601 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
    12661 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
    12721 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
    12781 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
    12841 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
    12901 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
    12961 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
    13021 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
    13081 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
    13141 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
    13201 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
    13261 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
    13321 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
    13381 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
    13441 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
    13501 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
    13561 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
    13621 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
    13681 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
    13741 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
    13801 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
    13861 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
    13921 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
    13981 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
    14041 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
    14101 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
    14161 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
    14221 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
    14281 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
    14341 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
    14401 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
    14461 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
    14521 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
    14581 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
    14641 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
    14701 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
    14761 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
    14821 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
    14881 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
    14941 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
    15001 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
    15061 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
    15121 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
    15181 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
    15241 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
    15301 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
    15361 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
    15421 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
    15481 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
    15541 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
    15601 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
    15661 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
    15721 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
    15781 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
    15841 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
    15901 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
    15961 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
    16021 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    16081 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    16141 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    16201 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    16261 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    16321 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    16381 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    16441 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    16501 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    16561 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    16621 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    16681 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    16741 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    16801 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    16861 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    16921 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    16981 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    17041 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    17101 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    17161 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    17221 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    17281 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    17341 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    17401 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    17461 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    17521 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    17581 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    17641 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    17701 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    17761 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    17821 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    17881 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    17941 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    18001 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    18061 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    18121 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    18181 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    18241 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    18301 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    18361 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    18421 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    18481 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    18541 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    18601 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    18661 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    18721 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    18781 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    18841 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    18901 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    18961 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    19021 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    19081 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    19141 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    19201 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    19261 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    19321 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    19381 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    19441 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    19501 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    19561 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    19621 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    19681 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    19741 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    19801 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    19861 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    19921 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    19981 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    20041 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    20101 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    20161 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    20221 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    20281 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    20341 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    20401 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    20461 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    20521 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    20581 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    20641 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    20701 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    20761 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    20821 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    20881 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    20941 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    21001 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    21061 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    21121 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    21181 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    21241 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    21301 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    21361 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    21421 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    21481 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    21541 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    21601 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    21661 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    21721 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    21781 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    21841 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    21901 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    21961 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    22021 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    22081 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    22141 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    22201 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    22261 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    22321 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    22381 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    22441 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    22501 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    22561 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    22621 tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag
    22681 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    22741 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    22801 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    22861 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    22921 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    22981 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    23041 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    23101 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    23161 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    23221 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    23281 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    23341 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    23401 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    23461 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    23521 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    23581 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    23641 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    23701 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    23761 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    23821 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    23881 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    23941 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    24001 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    24061 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    24121 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    24181 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    24241 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    24301 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    24361 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    24421 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    24481 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    24541 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    24601 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    24661 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    24721 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    24781 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    24841 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    24901 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    24961 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    25021 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    25081 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    25141 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    25201 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    25261 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    25321 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    25381 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    25441 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    25501 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    25561 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    25621 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    25681 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    25741 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    25801 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    25861 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    25921 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    25981 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    26041 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    26101 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    26161 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    26221 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    26281 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    26341 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    26401 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    26461 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    26521 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    26581 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    26641 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    26701 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    26761 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    26821 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    26881 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    26941 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    27001 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    27061 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    27121 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    27181 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    27241 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    27301 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    27361 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    27421 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    27481 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    27541 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    27601 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    27661 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    27721 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    27781 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    27841 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    27901 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    27961 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    28021 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    28081 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    28141 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    28201 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    28261 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    28321 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    28381 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    28441 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    28501 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    28561 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    28621 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    28681 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    28741 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    28801 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    28861 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    28921 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    28981 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    29041 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    29101 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    29161 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    29221 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    29281 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    29341 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    29401 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    29461 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    29521 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    29581 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    29641 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    29701 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    29761 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    29821 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    29881 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    29941 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    30001 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    30061 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    30121 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    30181 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    30241 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    30301 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    30361 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    30421 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    30481 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    30541 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    30601 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
    30661 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    30721 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    30781 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    30841 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    30901 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    30961 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    31021 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    31081 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    31141 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    31201 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    31261 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    31321 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    31381 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    31441 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    31501 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    31561 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    31621 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    31681 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    31741 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    31801 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    31861 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    31921 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    31981 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
    32041 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
    32101 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
    32161 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
    32221 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
    32281 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
    32341 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
    32401 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
    32461 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
    32521 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
    32581 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
    32641 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
    32701 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
    32761 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
    32821 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
    32881 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
    32941 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
    33001 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
    33061 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
    33121 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
    33181 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
    33241 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
    33301 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
    33361 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
    33421 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
    33481 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
    33541 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
    33601 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
    33661 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
    33721 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    33781 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
    33841 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
    33901 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
    33961 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
    34021 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    34081 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
    34141 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
    34201 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
    34261 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
    34321 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
    34381 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
    34441 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
    34501 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
    34561 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
    34621 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
    34681 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
    34741 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
    34801 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
    34861 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
    34921 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
    34981 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
    35041 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
    35101 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
    35161 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
    35221 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
    35281 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
    35341 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
    35401 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
    35461 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
    35521 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
    35581 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
    35641 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
    35701 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
    35761 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
    35821 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
    35881 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
    35941 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
    36001 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
    36061 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
    36121 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
    36181 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
    36241 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
    36301 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
    36361 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
    36421 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
    36481 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
    36541 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
    36601 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
    36661 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
    36721 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
    36781 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
    36841 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
    36901 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
    36961 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
    37021 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
    37081 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
    37141 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
    37201 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
    37261 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
    37321 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
    37381 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
    37441 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
    37501 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
    37561 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
    37621 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
    37681 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
    37741 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
    37801 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
    37861 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
    37921 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
    37981 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
    38041 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
    38101 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
    38161 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
    38221 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
    38281 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
    38341 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
    38401 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
    38461 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
    38521 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
    38581 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
    38641 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
    38701 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
    38761 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
    38821 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
    38881 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
    38941 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
    39001 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
    39061 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
    39121 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
    39181 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
    39241 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
    39301 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
    39361 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
    39421 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
    39481 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
    39541 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
    39601 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
    39661 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
    39721 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
    39781 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
    39841 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
    39901 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
    39961 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
    40021 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
    40081 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
    40141 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
    40201 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
    40261 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
    40321 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
    40381 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
    40441 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
    40501 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    40561 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    40621 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    40681 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    40741 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    40801 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    40861 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    40921 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    40981 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    41041 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    41101 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    41161 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
    41221 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    41281 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    41341 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    41401 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    41461 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    41521 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    41581 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    41641 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    41701 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    41761 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    41821 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    41881 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    41941 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    42001 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    42061 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    42121 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    42181 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    42241 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    42301 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    42361 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    42421 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    42481 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    42541 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    42601 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    42661 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    42721 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    42781 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    42841 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    42901 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    42961 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    43021 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    43081 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    43141 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    43201 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    43261 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    43321 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    43381 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    43441 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    43501 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    43561 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    43621 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    43681 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    43741 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    43801 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    43861 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    43921 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    43981 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    44041 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    44101 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    44161 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    44221 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    44281 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    44341 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    44401 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    44461 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    44521 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    44581 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    44641 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    44701 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    44761 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    44821 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    44881 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    44941 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    45001 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    45061 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    45121 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    45181 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    45241 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    45301 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    45361 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    45421 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    45481 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    45541 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    45601 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    45661 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    45721 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    45781 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    45841 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    45901 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    45961 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    46021 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    46081 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    46141 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    46201 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    46261 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    46321 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    46381 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    46441 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    46501 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    46561 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    46621 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    46681 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    46741 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    46801 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    46861 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    46921 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    46981 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    47041 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    47101 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    47161 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    47221 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    47281 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    47341 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    47401 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    47461 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    47521 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    47581 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    47641 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    47701 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    47761 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    47821 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    47881 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    47941 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    48001 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    48061 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    48121 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    48181 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    48241 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    48301 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    48361 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    48421 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    48481 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    48541 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    48601 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    48661 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    48721 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    48781 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    48841 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    48901 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    48961 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    49021 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    49081 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    49141 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    49201 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    49261 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    49321 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    49381 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    49441 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    49501 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    49561 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    49621 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    49681 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    49741 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    49801 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    49861 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    49921 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    49981 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    50041 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    50101 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    50161 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    50221 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    50281 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    50341 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    50401 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    50461 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    50521 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    50581 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    50641 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    50701 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    50761 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    50821 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    50881 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    50941 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    51001 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    51061 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    51121 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    51181 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    51241 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    51301 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    51361 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    51421 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    51481 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    51541 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    51601 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    51661 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    51721 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    51781 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    51841 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    51901 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    51961 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    52021 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    52081 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    52141 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    52201 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    52261 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    52321 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    52381 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    52441 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    52501 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    52561 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    52621 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    52681 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    52741 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    52801 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    52861 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    52921 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    52981 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    53041 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    53101 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    53161 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    53221 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    53281 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    53341 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    53401 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    53461 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    53521 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    53581 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    53641 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    53701 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    53761 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    53821 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    53881 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    53941 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    54001 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    54061 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    54121 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    54181 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    54241 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    54301 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    54361 aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta
    54421 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    54481 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    54541 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    54601 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    54661 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    54721 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    54781 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    54841 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    54901 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    54961 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    55021 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    55081 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    55141 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    55201 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    55261 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    55321 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    55381 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    55441 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    55501 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    55561 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    55621 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    55681 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    55741 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    55801 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    55861 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    55921 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    55981 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    56041 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    56101 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    56161 ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct
    56221 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    56281 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    56341 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    56401 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    56461 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    56521 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    56581 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    56641 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    56701 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    56761 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    56821 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    56881 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    56941 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    57001 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    57061 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa
    57121 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    57181 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    57241 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    57301 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    57361 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    57421 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    57481 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    57541 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    57601 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    57661 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    57721 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    57781 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    57841 ttcatttaaa agccggatca gtatttgaaa cagtaacaga attcaaaatc aaaacaagaa
    57901 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    57961 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    58021 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    58081 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    58141 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    58201 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    58261 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    58321 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    58381 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    58441 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    58501 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    58561 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    58621 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    58681 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    58741 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    58801 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    58861 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    58921 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    58981 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    59041 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    59101 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    59161 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    59221 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    59281 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    59341 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    59401 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    59461 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    59521 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    59581 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    59641 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    59701 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    59761 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    59821 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    59881 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    59941 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    60001 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    60061 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    60121 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    60181 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    60241 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    60301 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    60361 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    60421 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    60481 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    60541 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    60601 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    60661 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    60721 aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga cattgcaaga
    60781 aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca ttcagctcaa
    60841 tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca ttggtgaagc
    60901 ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg tacggtatat
    60961 tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag aagtgatcaa
    61021 cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt ggaattttgg
    61081 cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga ggttgtgaaa
    61141 aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct gtctatctat
    61201 ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta gagccctttt
    61261 tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc tttcttcaag
    61321 ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg ctgaatttgc
    61381 ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt caatacactc
    61441 atttgaccgg cattctctag gataacatgt ttggcatttt caagaaaaaa gcattcttgc
    61501 tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg
    61561 ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg attgaccttt
    61621 tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag cgcaatggca
    61681 aataggacat gccctacatg gacattgtca tcataggttg attcttctaa gatcccacct
    61741 aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt cttcccaagc
    61801 aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt aattgaaaaa
    61861 atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc tcacgaacac
    61921 gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg tgacactgta
    61981 gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt ttttatgctc
    62041 taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg agactttgcc
    62101 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
    62161 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
    62221 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
    62281 agattttgta ctccttcaat ttgtttctat aagctttcat ttgatacgcc caaccttgat
    62341 ctgagtgaaa cgtacgacga tagggacaat cgttagtggt ctggatagct tcctccaaac
    62401 cttccattac cgctaatgca ttgggctttt cagagatacg ataggataaa atctcactat
    62461 tgaacatatc cataaaagga tctagataca atttcttttg acggataatg cctttggcat
    62521 ccacttcatg gtatttaaat tcagttgtat ccgtcgtgat tttctgatgg cagatactgg
    62581 tgtagaaacg acgatggatc cgatttttgg ctactgctcc aacttttcct cgataagaat
    62641 tgtagcgacg tgatttacgc gtgtatgccg ttacttttat tcctagcttc ttgatcaagc
    62701 gctgaacttt ctttttattg atagagaaac cgcggtttct tagttcattg gtcatccgta
    62761 aacagccata atccttgtgc ttctcccgaa ttttaagcat ttccgcttca atctcttgat
    62821 ctggattttt acggtccaat cgcctttgcc atccaaataa tcatgaacga tctttaattt
    62881 aagttcaaaa gtatatttaa ccataaaaaa taccgacctc caaaagttag attttttggt
    62941 ctaacttttg ggggtcggta cacaaatcgt gtcacagtcc cttttttgaa aataaacggt
    63001 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctgtt
    63061 tttacttggc tgttcttatt acttgatgca aggcagcttt ttcacaacct cttttcaaat
    63121 atttcgtttt cttattctac aaatggttct tctcctaacg cttgttgagc ggctagattc
    63181 aacatgctcc attgtcggtc aaagcctggc tggaagaaga agtcagcttc tgctagttct
    63241 tctaaagtca gtttgttttg gatcgctagt gccaatacat ttccttgacc tgtaacatca
    63301 taggtagata atactgctcc tcctagcaat tgatgggtca ttggatccga gaataattgg
    63361 atggacactt ttggatttcc ttgttcttct ggtacatatt ttggtcgtaa cgtatcttca
    63421 taataagaca ctcgtacatc tactcctgct tttgctgctg taaagctatt caatccgctt
    63481 gctgcaaaat gatagtcaaa gacacttaat gcagaggaac ctacgactcc gccgaacggt
    63541 ttgtttggta cttcttcaaa aagatgtttt actacataac gtgcttctct acgtgcaaca
    63601 gtcgctaaag cgatcggcat ttttttgcgt gctgggatag aataagcaag tgtagcatct
    63661 cctactgcat acacatctgg aagattcgtt tgcaagtatt catttgtttg gatccagcca
    63721 cgttcatcta agtcgacgat cccttttaac cattctgtat ttgccttcac accggcagct
    63781 tgaatgacta gatctgctgg gatttctttt tcacttgttt tgattgcctc aacttttccg
    63841 ttaccagtaa aagcttcgat ttttgcattt gtcaaaactt caataccgtt ttcatttagg
    63901 tgttgttcta aaatatctgt catctcagca tctaaataag tgcctaaagg tctatcgatc
    63961 acatcaagca atacgacttc tttcccagct tttcggcttg cttctgctgc ttcgatccca
    64021 atgtaacccg cacctacgac agcgattttt ttaatagccg gatcatttaa tttttctttg
    64081 attttggttg cccaattgta tccacgcatc aagtagacat tttctagatc tgctcctggt
    64141 actggcagat tgtttggttt gacaccagaa ctcaaaatca atttgtcata aggatattct
    64201 tttgtttcgt tatctgatgc gctagtaacc gttactgttt ttttatcggc atcgatcgtt
    64261 tttacttcgt gattatttaa aatatgaaca ttggattgcg gaaagttttc tggtctgaaa
    64321 ttacgtacat catttacatc tgttaccttg tcttctaaat acagctccat accacatgac
    64381 atgaaggaaa tatagtctcc tgcttcaaat agcgtgatat ctacattttc accgtatcgt
    64441 gtcaatagtt ccaagatcga ttgatgccct ccatgagatg cacctacaat gacaactttg
    64501 attttttcca ttgatgaaac ctccttgaat attcttttaa aatcattcta aacaatttaa
    64561 acttaacatt acattagtta tgaatcaaaa gaaacgcgtt catttgaact aaatttaaaa
    64621 aacaatcgtc tccttgcaga atataccgca aaattaacga ttgttttttt gataaaatct
    64681 atgaatcact gttctgttaa aaagtctttg atccgtttgc aggcctcatg taatttttcc
    64741 atgctgctag cataagaaat ccggatataa ccttctcctt ctgggccaaa tgcaattcct
    64801 gggatacacg caacttttgc ctgttccgct aattctacac aaaactgcca agagtcagtt
    64861 tgaatggcag ctgggagttt gcaaaacaag taaaatgcac cgtttggctg ggtcacttca
    64921 aatcctaaag gcgccaattg ctggaccaga tagtctcttc gtgtttgata ttgctctttc
    64981 atattctcgc tatctttcac cccatttatt agcgcttcag ccgcagcttt ttgcgacatc
    65041 gttgtagcgg cagtcaccag atattgatgg actttaatta gttcgtcaat aagggctttt
    65101 tgagcaaaaa taaagcccac tcgccaacca gtcatcgcat gtgacttgga taacccgctt
    65161 atcacgattg tttgttctgg aagataagta gctatggaca tatgcgttcc actataagtc
    65221 aattcagcat atacttcatc acttataaca aataccggat atttttttaa aacctcagcg
    65281 attgctttca attgttttgc agacaatgtc gtcccagtag gattgttagg atagtttaaa
    65341 atgactgctt tgacagctga tccatattgt tcaaatgctc gttcaaattg ttccggctga
    65401 cagacaaacc ctgtgtcaga agtatctatc gggatcaatt cagcaccagc tagttccact
    65461 aatggctgat atcctgaata agcaggcgct ggaataagaa ccttgtctcc ttcttccaaa
    65521 acagataaca acgcagaggc aatcgcttcc gttgcaccaa ctgtcgtcaa aacttccgtt
    65581 tgcggatcat aagttaatcc ataacgctgt tgctgaaata tacaagccgc ttcccgcagt
    65641 tcctctaatc ctctcattcc ggtatagtga gaaaagtctt cttcaatcgc tgctattcct
    65701 gcttgcttga catgttcagg agttggaaaa tccggttctc ccagggtgag tttgatcaca
    65761 tctggtatcg acgatatttg ctgatcgaat tgtcgtattt ttgatacttc tattctagaa
    65821 agcctaggac taaatcgatt ggataaattc atcattattt actcgctcct cttttgaact
    65881 gctatcgatt tttacaagaa gattttctgt aaaaagaatc gttttgtacc attctacacc
    65941 attttctaaa acatccatat caaaatcgaa cgtcggatca tgtaaagcag gggtatcacc
    66001 gacacctaaa aagaaaaata cacatggata ttgttgcgta tatatgccaa aatcttcggc
    66061 ttgaagaact ggagcatcta attcatgtag tttgtttgaa tctaacagtg ctgcatataa
    66121 gcctctatgg ttgattacag ctggatagcc gtcattatag cgaaaagaaa tagtagctgt
    66181 agagtgaaac tgttctgcta aatggtctaa tcccctcttc aaataatttt gcatatctga
    66241 actgtaagaa cggatacttc cggatacaat aacttctcca gctaaaacat ttcggatcgt
    66301 tccaccttgg atttggccaa atttcattag atgattccac tctgccggta attgccggtc
    66361 aaaatcataa acattcttca aaaatcgtac agctgcctcc agactatcga ttcctttttc
    66421 actagaagca atatgtgcag actggccttt aatgataatg tctgtttcac tgctttgtgc
    66481 catcaaaggt cctggtcgag agaaaacttc tcccttagct agtccaggcc atagatgaaa
    66541 gccaaatatt gcttgtactt catatttatc caacaatcct gactgaataa cgctttgtgc
    66601 gccaccggaa atttcttctg atggctgata gatcagtact acattataaa taagatcctc
    66661 aatgtgttta gttataaagt cagtcaaccc taaaagcatc gccatatgtc cgtcatgacc
    66721 acatgcatgc atataacctt catgcttaga agcaaaagag agattcgttt cttctttgac
    66781 tggcagtgca tcgatatctg ttcgaaaagc aatcgtttct tcttgctgat tatcaaaata
    66841 aaggagtagt cctgtttcgc cgatttcatg gataacggcc tcgctgtttt ttacttgttc
    66901 atataaatat ttttttgtct tgaattcatg aaacccgatt tcaggaattt gatggagtgc
    66961 cattcgatat tctttcagca tccgtattca ccatcccttc atagataccc ttcactactg
    67021 tttcatattg attattcggt acaccgatga tcagattcaa ctcctgtgct ccttgtgata
    67081 agatcgatgt gcgaatttct aatttgttca atatcgataa cactttccca gataatccaa
    67141 tcaattcttt atgcggaccc ccaacgacag agatcaaagc taagtcttcg atcacgacga
    67201 tttcttccac gccaagtgtt tcttttaatt ctttcgtgat gagaaataac ttatcggcaa
    67261 tcgcttctgc tgagacgatc actccgatat tgtcgatccc tgaaggaata tgttcgattg
    67321 atatctcatg tttagcaaaa atgctcattg ctttccagat aaacccgatt tcatcggaca
    67381 tatgacgttt gaataaagtg atggaaagaa aatccttgcg tccagctatt cctgtcagtc
    67441 cattttcctt ttctatttcg tgattcgata tttttgtccc ttgagcgaaa ggatcattgg
    67501 tattttttat ttggatgggg atatcagcct cttgaactgg gaaaacagct gcttcatgaa
    67561 gaacgttagc ccccatataa gccatctctc tcagttctct aaaagagatt tcctcgatga
    67621 ttttaggatg atcgatgatt cgcggatcag ccatcatgat tcctgaaaca tccgtccaat
    67681 tctcataact ttccgcttgc aacatcttcg ccagtatcgc gccggtaatg tcgcttcccc
    67741 cacggcccaa taatttttct tttcctgcag ggttcgcccc gtaaaaccct ggaacaacca
    67801 ttttcggata tcttttaaat gcttttgtga tttcctgtgc tgttttttcc tgatcgattt
    67861 tcccatcata gtcaaagaaa atcaggtctt ttgcgtcaat aaactggtaa ccaagatact
    67921 cagccatcag ttgagcagtc agatattcac ctctacttac taaataatct tcagttatct
    67981 tttcgtcttc cagttgtttc tgtatgcgtg ctagttcatc ttcaataggt aactgcaggg
    68041 caagaacatc tcttacttga atgaaacgat cacaaatcct tttccaaagg ggtgtccaat
    68101 caacatgatg ctgaaggtaa gcataaatca aataaagtaa gtctgttact ttgtcatcat
    68161 ctgacgtacg tttgcctaaa gcagaaacaa cgatcacttg gcgattggga tctgaaagaa
    68221 caattgtacg gacttttttg aattgctctt catgagacat cgatgttccg ccgaatttcg
    68281 ctactttcag cgtattactc atcttgccac ctccgctatt ttctgcatta aagctgcagc
    68341 aatctgtacg gcattcgcag cggctccttt gcgtatgtta tcggcagtcg tatagaacaa
    68401 caatccgttc tctaaagaag tgtcttttct gatcctccca acataaacca tatctgttcc
    68461 tctagcaaga atgctcgtcg gatatttatg tgctgctaga tcatcttcta attgaatccc
    68521 tggaaactgg ctgagacact ctctaatttc ttccgtcgta aactcttttt gcagttgaac
    68581 tgccacagag actccatgtc cattttcgat tggcacacgt acgcaggttg ctgatactgg
    68641 caaagcaggc tgatgcagaa tcttacgggt ctcatttatc attttctgtt cttcttttgt
    68701 atacccattt tccaaaggct gatcgatttc cggaatcacc gtttggctaa tattataagg
    68761 atacaaaact ggttcctcgt cattttgcgt acgatgaaga tcttcaatgc ctttttgccc
    68821 ggaaccagaa actgcttgat aggtcgaata attgactcgc tggataccaa aggcttcttg
    68881 taaagcttta agcggaagta ccgcttgaat ggttgaacaa tttggatttg caatcagccg
    68941 attcaatgta tagtcttcca gattgacctc tggaacgatt aatggaatat ccgaatcttc
    69001 ccgccatgct gaggaattat ctatgacaaa tagtccctga cttcgtgcga ttggcgcaaa
    69061 ctctttggat acagaagcac ctgcagaaaa tagcgctaaa tcatatcctt caaatgaagt
    69121 ttctgtcaat tcctgtacag tgtgtttctt tttcttgaat acacaaactt taccggctga
    69181 acgttttgaa gctaataatt tcaatcgtcc tactggaaaa tcatattctt ctaacacttt
    69241 gagcatcgtt tggccgacta atccggttgc ccccacaata caggtatcta tttcgcgcat
    69301 ttttctccat ctcctctata attcgcgtaa cgcagcaatc aatgctgtat tttcttttgt
    69361 ctgatcatca cttattttta atacacgtgc aggtattcca ccgacaacag tttctgctgc
    69421 cacatcttct aaaaccactg ctcctgctgc cacgactgca tcttttccga tatggacacc
    69481 ttcaacaatc actgcattcg cacctaccag tacgccgtct tctacgataa ccggtttggc
    69541 cgatgctggt tcgatcacac ctgctaagac agctcctgca ccaatatggc agtttttgcc
    69601 cactgtcgct cgtccaccta agacagcacc catatcaatc atcgtgtttt caccgattac
    69661 tgcaccaata ttgataatcg ctcccatcat gatcacggca ttgttgccga ttgacacttg
    69721 atctctgata atagcacctg gttcgatacg agcattaatt tttttaatat ctagcaaagg
    69781 aactgcgctg tatcttgcag cagtttcgat ttcgatttca ataatttttt ctgattcagc
    69841 ttctaaaacc ggagcaatcg tctcatagtc accaaaaaca acgaagctgc ttccttcacc
    69901 aaaaacttta ctttctttaa attcgattgg ctcggttaca ttcagataga ctttcacagg
    69961 cgtcttttta gatgccgttt gaatgtagcg gatgatttct tgagcttcca cgttttttac
    70021 acccctttgt ccattaataa atcttctaat ttgtaataac ctggtgactg atctttcaac
    70081 cagtccgctg cttctactgc gcctgcagca aagattcgat tagaaaaaga ttcatgtttg
    70141 atcgtaaata tttcatcagt agcagcaaac aatacatcat gttcaccagg taaagaacct
    70201 gcacgaatac tgtgtacacc aatttggttt tcttctcttt ttttcccttg ccagttgtat
    70261 acgggctgca gcttttgcac ttcttggatt gtttgaagca gcatatttgc agtaccagat
    70321 ggtgcatctt ttttacgact atgatgtttt tcaatcagtt cgatctgcca gccttgcaac
    70381 attgcagctg cttgcttgat tagttgattc atcaccatga tgccgaacga aaaattagca
    70441 ctatacatca caggtacatg tttggcatgt tcttttattt cttggaactg tgattgtgta
    70501 taacctgtcg tgccaatgac taacggaagt tgatatgata agctatacga aaggattttc
    70561 tctaaattat ctggatgaga aaaatcaata agcagttctg gcagttccgt aaaagcaagc
    70621 gtagttgctt ctttaggtgc cgtccgtaaa agaaggaccg tatctcctct ttcttgtgca
    70681 acttcttcga ctctatttcc catctgtcca ctgccaatca gtccgatttt catttttcaa
    70741 ccgtcctttc ttttaacaaa agctgagcta attgtttctt tttctctttt gaaagagaaa
    70801 ctaaaggcag gcgtagaggt ccggaaggta agtctaataa tgacatggct gctttgacag
    70861 gaataggatt ggtctcactg aacaaggcat ggatcagttc aagtgcattt aattgctttt
    70921 cttttgcttg ctgccatctt ccgtcctgcg tatcttttac taattcatgg actattttag
    70981 gttggatatt cgcccataca cttattacac cgcttcctcc tacactcatt actggtagga
    71041 tcaaatcatc atttccactg taaaggaaga attcttctcc aatcaaccga gcggcatcca
    71101 tcacataagc catatctcct gaggcttctt tcaaaccaat gatattagga tgttctgcta
    71161 aatttactaa tacatggatt ggaatcgaca tgcctgttct agaaggaaca ttatacaaaa
    71221 taagcggaat cggtgaggct tctgcaattg ctgtaaaatg agcagctagt cctgcatcac
    71281 ttgttttgtt gtagtaaggg gtgatgacca aaagtgcgtc tgcccccaaa gaagcaaaat
    71341 gcttcgcttt ttcaatcgtt tctttcgtat tattcgttcc agctcctgca atcaccggca
    71401 cacggccagc cactttcttt actgtcagtt gaaggatctg ctcctcctca gattgtgtga
    71461 gcgtagaaga ttctcctgtt gtacctaaaa ctaataatcc atctgtttgt tcatctaaat
    71521 ggaattggat caatttttcc aaaccagcat aatctactga tccctcttca ttcatcggcg
    71581 tgatcagcgc cacgatcgaa ccttttatca tcttcattcc tccaatattc ttgtccattc
    71641 atgaactgct gggccagcca tttgtatcgc atcctcacca gagatcatca agtctccttg
    71701 ttccaagtga acaactacct gatctgctga aattttccca gtttccttag caaccacata
    71761 agaggcacag caacctgtac cgcaagcaag tgtccaacct actcctcttt cataggtacg
    71821 gacgatcaat tcttgttcat tgactacttc tacaaagttg acattggttt tttctttgaa
    71881 caaaggatga tgacagatca gttcacctct ttttctccag acttcctcac gcgcattttc
    71941 aacaaaaaca acggtatgaa tcgttcctaa aaataaacta gtgatcgtta ctttttcacc
    72001 attgatctct atttcctggt tttttattgg ataagtttga ttagctttga ttttttccgg
    72061 agaatcatct ggcactccca tatctacgct gcaataaaaa ggtgagggat ccaacaccgt
    72121 aatttttctt gtgcctgcta atgtctctac gttgaattcc tgttcattca tcatgttctg
    72181 atcatttacg tatcgagcaa aacagcgaat gccatttccg cacatcggcg cgcggctgcc
    72241 atctttgttg taatagatca tttccaacgg atcgattttt acggcgatca atccgtctat
    72301 atcatatttt tcttttgtgc ataaagaagc ggctaattga ctataatctg tgtcttctgc
    72361 gtaatctaat aagataaaat catttccgca gccattatat ttatgaagga tcataggaaa
    72421 cttcccccct tagtagatca gaaaatgctt gtctgcgaac aatcagtctt gattggccat
    72481 tattgacaaa aacaactgct ggtcgtgtcg ctcgattgta gttgctgctc atcgagtatc
    72541 cataagctcc cgtggaataa acagctaaaa gatcacccga ttctgctttc ggcaaataag
    72601 cttctttaat taccacatcg cctgattcac acatttttcc tgcaatcgtc actttctctg
    72661 ttttttcttc cgacagtttt gctgctaaat cacaatcgta ttctgcctga tacagagctg
    72721 gacggatatt atctgtcatc ccgccatcta caaagtaata ttttttatgt ggtgtctttt
    72781 tgatgaaacc aatcgtgtat agtgttgtac cagcttctcc aacaatactt ctacccggtt
    72841 cgatcagtaa ttgacgaatg gtcagttttc gtttttccaa agcttcttcc gtatattcaa
    72901 tcagtttttt tagtgcctct tcgactggga tcggctgatc attttgtgta taacgaatcc
    72961 caaaccctcc gccaagattc aaggaaagag gttggtcaaa atcggttaag taattcgtca
    73021 atttgtcgat ttcagctaac caagcagtaa catcaaaaat ttgtgacccg atatgagcat
    73081 gaaatccttc taactctaga tagctgcttt cttttatcaa ctgaagggct tgttggcact
    73141 ctttactttc atagctcatg ccgaattttg aatcaatatg cgcagtgacg atatattcat
    73201 gagtatgtgc ttctactccg acattcaacc gaagcatgat acagattttt tttaaatagc
    73261 gttggcttaa ttctgctaat aattcaactt ccatcaaatt atcggcaaca atatgcaaca
    73321 aatcattttc aagtgcatag atcagttcct ctggcgtctt gttgttacca tggaagtaga
    73381 tagtatctct aggaaaatca gcttgcaaag cggtatagat ttccccacca ctgacaacat
    73441 ctagcccgaa tccatattca cgaactaaat gcagcatctc aatcgtttgg aatgctttag
    73501 aggcgtaaag cacttttgta tcaaaacgtt tcgatacaaa gcctcgcttg aatgacagca
    73561 tcgtttgtct catcttttct tcatcataca catacagagg tgtcttatac ttttctgcca
    73621 atgtgcttgc tgtgactccg ccaattatca attctgtcat tttttcattc ctcttttcac
    73681 tttttttccg tttttattgg aatttcattt gataaagatt tgaattttat tggaataata
    73741 ttaaacaaaa ttttctgaat tgtctagtat atttcgaaat atttttttaa aaataagaat
    73801 aaaaatggtt ataaaaacaa aatacccgaa ctattagaag ctatttatac ataaaaacat
    73861 aaattcaaca ctatttagtg atgaaaaaat attttcgctt caatatagtt cggaatagtt
    73921 cggatatttt caattttatg aatcatttca atttcttcat aaataagtgc agttttttct
    73981 ttctctagct tttgccattc atctttcaaa atataaataa aatcaaatac ttttttattt
    74041 ttcaatttgc tgattccaca aaaaacctct tgaaaggcag atccgatgat ctgcctttca
    74101 agaggtcaag ctctgttttc aaattcttat caatccgtta gcgaattatt ttgacgcacg
    74161 ttttacataa gaaccttctg ctgtattgat ttccaacaat tcatctgttt cgatgaagtc
    74221 aggaacattg actactaagc cagtttccat tgtcgctggc ttacctgaac cagtgactgt
    74281 tgcacctttg atggaaggtt gtgtttccac aacacgtaat acgacagttg ttggtaattg
    74341 aacgccgatg acttcgctac cgaaaaattg gatttttact tccatgtttt ctaggatata
    74401 tttcatttct tcttctactg tttctacagg aatttcatat tgctcatatg tttccaaatc
    74461 catgaagaaa gctgtatcat ccatcgtgta caaatattgt acaggttttg tgtcgatatg
    74521 tgctttttca aatttttctt ctggtctgaa cgttgtgtcg tatgttgcac ctgaacggac
    74581 atctttcagt ttcatacgca taactgtatt tcctttacca ggtttatggt ggcttgcatc
    74641 cagaacttta atcagtttgc cgtcttgtac gaaagtcata ccggctctta aatcacttgc
    74701 tgagatcatt atttttgctc cttcttttct ttatcataat caactttatt gtatcagaca
    74761 accggccaga aaactagagt attctctatc ttttaaggtc tcatgcctat ttgtcagaaa
    74821 aataaacacg ggtaaacgat gctatcgaaa tatttttttc tttcaagcct ttgactttta
    74881 ttttttttca tgctatcgtt aataaaaatt ttacttggag ggatacacat gacagtcagc
    74941 aaccaaaaac aattgaatcg ttgctgttgt atggtgcata aaagcagcat actatttgtc
    75001 atgtctatta attgcccttc atagaatgag taccaggtaa cttatctttt tatctactca
    75061 atttttctct atgtctttca aggagctgat ttaatagtca gctccttttt ttattatctt
    75121 tttttaggag gcccacatga ttatctcgac aattacagaa gctattggtt ccacaccgct
    75181 ttatcgcttt gacaatcagc actattctat gcctaaaggt tctgctattt atgcaaaact
    75241 agaatattta aatcctggag gaagtatcaa agatagattg ggcagttatt tgatcaaaga
    75301 aggatttgcc caaggaaaaa tcaatgaaaa aacgacgatc atcgaaccaa ctgccggaaa
    75361 tacaggaatt gggatcgctc tggccgcact cacatacaaa ttaagaacgg tttttgttgt
    75421 tccagagaag tttagcttag aaaaacaaca attgatgaaa gcattgggag caaagatcat
    75481 ccattctcct agtgagcaag gaatcatcgg tgccatcagc aaaagtaaaa aacttgcaga
    75541 ggagatatca aacagctatc tgcctcttca atttgaaaat aaagataatc ctgctgctta
    75601 ctatcatacg ttagggccag aaatctttca tgaaatcaaa gaaaatatcc atagttttgt
    75661 agcaggaatt gggagtggtg gaacctttgc tggaacttct acctttttga aagaaaaata
    75721 tcccgacatt cgaataatcg gtgtagaacc agagggctct gtgttaaatg gtggtgatcc
    75781 agcccctcac gaaattgagg gtatcggtgt agaattcatt ccgccgtttc tttctcccct
    75841 ttcaatcaat cagatcgaga caatctctga tgtagaaggg tttaactata ctaggcagtt
    75901 agcacgagaa caaggactat tagttggaag ttcgagtggt gcggcttttg cagcagcatt
    75961 aagagaaatt cgtcgtttac cacctggtca tcgtgtcgtt acgatttttc cagacgcagc
    76021 agaccgctat ttatcaaaaa acatttactt ataggaggaa agaaacatgc acattcaaac
    76081 aaaattgatc cacggaggca tcagtgaaga tcccacaaca ggagctgtca gtgtacctat
    76141 ttatcaaaca tccacttatc ggcaagatgg cgtcggccag cctaaacaat atgaatattc
    76201 aagatctggt aatcctactc gttttgcatt ggaagaactg attgctgatt tggaaggcgg
    76261 tgttcgagga ttcgcttttt cttctggtct ttctgggatt catgccgttt tttctctttt
    76321 tcaagctggc gatcatatct tattaggaga tgatgtctat ggaggaacat tccgtctatt
    76381 tgacaaagtt cttactaaaa atggtctcga gtacacgatc atagatacga gtaatcttga
    76441 taaaatagaa caatccatca aacccaatac aaaagcactt tatcttgaaa cacctagcaa
    76501 tccgcttcta aagattactg atttagaaaa aagtgccact ctggctcatc aacatggatt
    76561 gatagttatt gctgacaata catttgctac tccttatttt caaagacctt tagatctcgg
    76621 ttctgatatc gttgtccata gcggtaccaa atatctaggc ggacacagcg atgtagtagc
    76681 tggtttagtg acttctaacc ataaagatct agccgatcag atcggatttt accaaaatgc
    76741 tatcggagcc gtacttggtc cccaagatag ctggttactt cagcgcggaa tcaagacact
    76801 aagtgttcgt atggaggaac atcaaaaaaa tgcttttgtg gtagctgatt ttctattttc
    76861 tcatccagca gtagaaaagg tctactatcc aggattaccc gatcatgaac tccatggagt
    76921 tgctaagcag cagatgagcg gctttagcgg aatgatttct tttactttaa aaaatgaaga
    76981 atctgctatt ccatttgttg aatcactcca actatttact ttagcagaaa gtttaggtgg
    77041 cgtagaaagt ttagtggaaa ttccttctgt catgactcat gcgtctattc caaaagaaaa
    77101 acgagaagag gctggcataa aggacggctt gatccgtctt tctgtaggaa ttgagtatgg
    77161 tcaggatctc ataaacgatt tagctcaggc atttgacaga atcaagaact agaagtccca
    77221 ataaacgact tagcagaaaa atctaaaaaa tatttcagat tgctacacct tggaaacgat
    77281 aagtgttaga atcaagtaga aagacgtttt tgtaaatttc actaaataat tctttagtga
    77341 aaagcatcaa aacgaatgaa caaacagata ggagtcttac aaatgagaat gaatgtcgaa
    77401 agttttaatc ttgaccatac aaaagtcaaa gcaccttatg tacgtgtagc tgatcgaaaa
    77461 acgggtgcgc atggcgatgt catcattaaa tacgacgtcc gctttaaaca accaaataaa
    77521 gaacacatgg acatgccaag tctacattca cttgagcact tgactgccga actaatccgc
    77581 aatcacgcag attacatcgt agactggtct ccaatgggat gccaaacagg cttttacttg
    77641 actgtattga atcatgaaag ctacgaagac attttagatg ttcttgaagc aacaatgaaa
    77701 gatgttcttg aagctactga agtacctgca agcaatgaaa cacaatgtgg ctgggctgcc
    77761 agccatacac tagaaggtgc acaagctttg gctcgtgaat ttttagcaaa acgcgatgaa
    77821 tggtctgaag tagaagcata gtttctaaaa gaacgtaaag atgagaagca atatagctat
    77881 cttatcttcg tattttatta aatatttatt gtctgaaaag agttctaaac gggaaaacaa
    77941 atagctagtt ttcagtttag cgctcttttt attatataaa atagaaagga gaaaacttta
    78001 tgtctattta taattttgaa actactttag aagacggcac tgtctattca ttgaaaaaat
    78061 atgcaggcaa accgataatc attgttaaca ctgcaacaaa atgcggtcta gcacctcaat
    78121 ttaaacaatt agaagaactt taccaagctt atcagacaca aggtctggtt atcttaggct
    78181 ttccttccaa tcaatttaaa caagagctag attcagcgac tgaagcagca gaagcttgta
    78241 aaaccactta tggcgttacc ttccctatgc atcaattgat tgacgtaaat ggagaaaata
    78301 cagatcctct ttttacttat cttaaaaatg aagctcctgg tactgtcact aaagcaataa
    78361 aatggaattt tactaaattt ttgattgata gaaatggcaa agtaatcgaa cgatttgcac
    78421 cacaaacttc tccattgaaa atgaccgctg caattgaatc cgttctttaa cttaataaaa
    78481 ataaaaaaag atgaactatt tctagttcat ctttttgcct ttgcgtggcg acgtcctact
    78541 ctcacaaggg gcaacccctc actacaatcg gcgctaagaa gcttaacttc tgtgttcggc
    78601 atggttacag gtgtatcctt ctcgctatcg ccaccacact gtggtgttat cttttattga
    78661 gtaaattttg ttcactcaaa actggatttg aagtca
//
LOCUS       DACIWG010000004        77135 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.004, whole genome shotgun sequence.
ACCESSION   DACIWG010000004 DACIWG010000000
VERSION     DACIWG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.004"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYF54_000235"
     CDS             complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYF54_000235"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ9830604.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1645..2568)
                     /locus_tag="IYF54_000236"
     CDS             complement(1645..2568)
                     /locus_tag="IYF54_000236"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ9830605.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2764..3369
                     /locus_tag="IYF54_000237"
     CDS             2764..3369
                     /locus_tag="IYF54_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9830606.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3677..4693
                     /locus_tag="IYF54_000238"
     CDS             3677..4693
                     /locus_tag="IYF54_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9830607.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4834..6135
                     /locus_tag="IYF54_000239"
     CDS             4834..6135
                     /locus_tag="IYF54_000239"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ9830608.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6181..6843
                     /gene="deoC"
                     /locus_tag="IYF54_000240"
     CDS             6181..6843
                     /gene="deoC"
                     /locus_tag="IYF54_000240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ9830609.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6864..7262
                     /locus_tag="IYF54_000241"
     CDS             6864..7262
                     /locus_tag="IYF54_000241"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ9830610.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7328..8410
                     /locus_tag="IYF54_000242"
     CDS             7328..8410
                     /locus_tag="IYF54_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9830611.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8622..10184
                     /locus_tag="IYF54_000243"
     CDS             8622..10184
                     /locus_tag="IYF54_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830612.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10177..11310
                     /locus_tag="IYF54_000244"
     CDS             10177..11310
                     /locus_tag="IYF54_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830613.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11310..12266
                     /locus_tag="IYF54_000245"
     CDS             11310..12266
                     /locus_tag="IYF54_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830614.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12365..13531
                     /gene="deoB"
                     /locus_tag="IYF54_000246"
     CDS             12365..13531
                     /gene="deoB"
                     /locus_tag="IYF54_000246"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ9830615.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13555..14373
                     /locus_tag="IYF54_000247"
     CDS             13555..14373
                     /locus_tag="IYF54_000247"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ9830616.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14390..15097
                     /gene="deoD"
                     /locus_tag="IYF54_000248"
     CDS             14390..15097
                     /gene="deoD"
                     /locus_tag="IYF54_000248"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ9830617.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="IYF54_000249"
     CDS             complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="IYF54_000249"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ9830618.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16253..16834)
                     /locus_tag="IYF54_000250"
     CDS             complement(16253..16834)
                     /locus_tag="IYF54_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9830619.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(16967..17650)
                     /gene="rpiA"
                     /locus_tag="IYF54_000251"
     CDS             complement(16967..17650)
                     /gene="rpiA"
                     /locus_tag="IYF54_000251"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ9830620.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            17969..18382
                     /gene="rpsL"
                     /locus_tag="IYF54_000252"
     CDS             17969..18382
                     /gene="rpsL"
                     /locus_tag="IYF54_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ9830621.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18466..18936
                     /gene="rpsG"
                     /locus_tag="IYF54_000253"
     CDS             18466..18936
                     /gene="rpsG"
                     /locus_tag="IYF54_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ9830622.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19030..21117
                     /gene="fusA"
                     /locus_tag="IYF54_000254"
     CDS             19030..21117
                     /gene="fusA"
                     /locus_tag="IYF54_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9830623.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21289..22476
                     /gene="tuf"
                     /locus_tag="IYF54_000255"
     CDS             21289..22476
                     /gene="tuf"
                     /locus_tag="IYF54_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ9830624.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22883..23191
                     /gene="rpsJ"
                     /locus_tag="IYF54_000256"
     CDS             22883..23191
                     /gene="rpsJ"
                     /locus_tag="IYF54_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ9830625.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23222..23851
                     /gene="rplC"
                     /locus_tag="IYF54_000257"
     CDS             23222..23851
                     /gene="rplC"
                     /locus_tag="IYF54_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ9830626.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23879..24502
                     /gene="rplD"
                     /locus_tag="IYF54_000258"
     CDS             23879..24502
                     /gene="rplD"
                     /locus_tag="IYF54_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ9830627.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24502..24792
                     /gene="rplW"
                     /locus_tag="IYF54_000259"
     CDS             24502..24792
                     /gene="rplW"
                     /locus_tag="IYF54_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ9830628.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24831..25664
                     /gene="rplB"
                     /locus_tag="IYF54_000260"
     CDS             24831..25664
                     /gene="rplB"
                     /locus_tag="IYF54_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ9830629.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25707..25985
                     /gene="rpsS"
                     /locus_tag="IYF54_000261"
     CDS             25707..25985
                     /gene="rpsS"
                     /locus_tag="IYF54_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ9830630.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26008..26355
                     /gene="rplV"
                     /locus_tag="IYF54_000262"
     CDS             26008..26355
                     /gene="rplV"
                     /locus_tag="IYF54_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ9830631.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26369..27025
                     /gene="rpsC"
                     /locus_tag="IYF54_000263"
     CDS             26369..27025
                     /gene="rpsC"
                     /locus_tag="IYF54_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ9830632.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27028..27462
                     /gene="rplP"
                     /locus_tag="IYF54_000264"
     CDS             27028..27462
                     /gene="rplP"
                     /locus_tag="IYF54_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ9830633.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27452..27640
                     /gene="rpmC"
                     /locus_tag="IYF54_000265"
     CDS             27452..27640
                     /gene="rpmC"
                     /locus_tag="IYF54_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ9830634.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27665..27931
                     /gene="rpsQ"
                     /locus_tag="IYF54_000266"
     CDS             27665..27931
                     /gene="rpsQ"
                     /locus_tag="IYF54_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ9830635.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            27989..28357
                     /gene="rplN"
                     /locus_tag="IYF54_000267"
     CDS             27989..28357
                     /gene="rplN"
                     /locus_tag="IYF54_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ9830636.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28394..28702
                     /gene="rplX"
                     /locus_tag="IYF54_000268"
     CDS             28394..28702
                     /gene="rplX"
                     /locus_tag="IYF54_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ9830637.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28730..29269
                     /gene="rplE"
                     /locus_tag="IYF54_000269"
     CDS             28730..29269
                     /gene="rplE"
                     /locus_tag="IYF54_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ9830638.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29288..29473
                     /locus_tag="IYF54_000270"
     CDS             29288..29473
                     /locus_tag="IYF54_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ9830639.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29509..29907
                     /gene="rpsH"
                     /locus_tag="IYF54_000271"
     CDS             29509..29907
                     /gene="rpsH"
                     /locus_tag="IYF54_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ9830640.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29939..30475
                     /gene="rplF"
                     /locus_tag="IYF54_000272"
     CDS             29939..30475
                     /gene="rplF"
                     /locus_tag="IYF54_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ9830641.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30636..30992
                     /gene="rplR"
                     /locus_tag="IYF54_000273"
     CDS             30636..30992
                     /gene="rplR"
                     /locus_tag="IYF54_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ9830642.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31013..31513
                     /gene="rpsE"
                     /locus_tag="IYF54_000274"
     CDS             31013..31513
                     /gene="rpsE"
                     /locus_tag="IYF54_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ9830643.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31528..31707
                     /gene="rpmD"
                     /locus_tag="IYF54_000275"
     CDS             31528..31707
                     /gene="rpmD"
                     /locus_tag="IYF54_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ9830644.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31751..32191
                     /gene="rplO"
                     /locus_tag="IYF54_000276"
     CDS             31751..32191
                     /gene="rplO"
                     /locus_tag="IYF54_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ9830645.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32191..33486
                     /gene="secY"
                     /locus_tag="IYF54_000277"
     CDS             32191..33486
                     /gene="secY"
                     /locus_tag="IYF54_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ9830646.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33548..34195
                     /locus_tag="IYF54_000278"
     CDS             33548..34195
                     /locus_tag="IYF54_000278"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ9830647.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34386..34604
                     /gene="infA"
                     /locus_tag="IYF54_000279"
     CDS             34386..34604
                     /gene="infA"
                     /locus_tag="IYF54_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ9830648.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34637..34753
                     /gene="rpmJ"
                     /locus_tag="IYF54_000280"
     CDS             34637..34753
                     /gene="rpmJ"
                     /locus_tag="IYF54_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ9830649.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34771..35136
                     /gene="rpsM"
                     /locus_tag="IYF54_000281"
     CDS             34771..35136
                     /gene="rpsM"
                     /locus_tag="IYF54_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ9830650.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35164..35553
                     /gene="rpsK"
                     /locus_tag="IYF54_000282"
     CDS             35164..35553
                     /gene="rpsK"
                     /locus_tag="IYF54_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ9830651.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35633..36571
                     /locus_tag="IYF54_000283"
     CDS             35633..36571
                     /locus_tag="IYF54_000283"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ9830652.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36599..36979
                     /gene="rplQ"
                     /locus_tag="IYF54_000284"
     CDS             36599..36979
                     /gene="rplQ"
                     /locus_tag="IYF54_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ9830653.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37251..38090
                     /locus_tag="IYF54_000285"
     CDS             37251..38090
                     /locus_tag="IYF54_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9830654.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38066..38935
                     /locus_tag="IYF54_000286"
     CDS             38066..38935
                     /locus_tag="IYF54_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9830655.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38925..39722
                     /locus_tag="IYF54_000287"
     CDS             38925..39722
                     /locus_tag="IYF54_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ9830656.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39925..40671
                     /gene="truA"
                     /locus_tag="IYF54_000288"
     CDS             39925..40671
                     /gene="truA"
                     /locus_tag="IYF54_000288"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ9830657.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(41004..43673)
                     /locus_tag="IYF54_000289"
     CDS             complement(41004..43673)
                     /locus_tag="IYF54_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ9830658.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(43911..44099)
                     /locus_tag="IYF54_000290"
     CDS             complement(43911..44099)
                     /locus_tag="IYF54_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ9830659.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44369..44731
                     /locus_tag="IYF54_000291"
     CDS             44369..44731
                     /locus_tag="IYF54_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9830660.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            44783..46465
                     /locus_tag="IYF54_000292"
     CDS             44783..46465
                     /locus_tag="IYF54_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ9830661.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46582..48618
                     /gene="recG"
                     /locus_tag="IYF54_000293"
     CDS             46582..48618
                     /gene="recG"
                     /locus_tag="IYF54_000293"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ9830662.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            48667..49668
                     /gene="plsX"
                     /locus_tag="IYF54_000294"
     CDS             48667..49668
                     /gene="plsX"
                     /locus_tag="IYF54_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ9830663.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            49790..50032
                     /gene="acpP"
                     /locus_tag="IYF54_000295"
     CDS             49790..50032
                     /gene="acpP"
                     /locus_tag="IYF54_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ9830664.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            50300..51304
                     /locus_tag="IYF54_000296"
     CDS             50300..51304
                     /locus_tag="IYF54_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830665.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            51305..52249
                     /locus_tag="IYF54_000297"
     CDS             51305..52249
                     /locus_tag="IYF54_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830666.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            52249..53211
                     /locus_tag="IYF54_000298"
     CDS             52249..53211
                     /locus_tag="IYF54_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830667.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            53238..54152
                     /locus_tag="IYF54_000299"
     CDS             53238..54152
                     /locus_tag="IYF54_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830668.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            54178..55959
                     /locus_tag="IYF54_000300"
     CDS             54178..55959
                     /locus_tag="IYF54_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9830669.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            56307..56993
                     /locus_tag="IYF54_000301"
     CDS             56307..56993
                     /locus_tag="IYF54_000301"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ9830670.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            57013..60594
                     /gene="smc"
                     /locus_tag="IYF54_000302"
     CDS             57013..60594
                     /gene="smc"
                     /locus_tag="IYF54_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ9830671.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            60603..61412
                     /locus_tag="IYF54_000303"
     CDS             60603..61412
                     /locus_tag="IYF54_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9830672.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            61423..62421
                     /gene="ftsY"
                     /locus_tag="IYF54_000304"
     CDS             61423..62421
                     /gene="ftsY"
                     /locus_tag="IYF54_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ9830673.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            62608..64875
                     /gene="gshAB"
                     /locus_tag="IYF54_000305"
     CDS             62608..64875
                     /gene="gshAB"
                     /locus_tag="IYF54_000305"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ9830674.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(64964..65416)
                     /locus_tag="IYF54_000306"
     CDS             complement(64964..65416)
                     /locus_tag="IYF54_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ9830675.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            65566..66516
                     /locus_tag="IYF54_000307"
     CDS             65566..66516
                     /locus_tag="IYF54_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ9830676.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            66509..67468
                     /locus_tag="IYF54_000308"
     CDS             66509..67468
                     /locus_tag="IYF54_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ9830677.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            67465..68220
                     /locus_tag="IYF54_000309"
     CDS             67465..68220
                     /locus_tag="IYF54_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9830678.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSTLRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            68243..69196
                     /locus_tag="IYF54_000310"
     CDS             68243..69196
                     /locus_tag="IYF54_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9830679.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(69840..70136)
                     /locus_tag="IYF54_000311"
     CDS             complement(69840..70136)
                     /locus_tag="IYF54_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ9830680.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            70491..70847
                     /locus_tag="IYF54_000312"
     CDS             70491..70847
                     /locus_tag="IYF54_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9830681.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            70857..71756
                     /locus_tag="IYF54_000313"
     CDS             70857..71756
                     /locus_tag="IYF54_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ9830682.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            72273..73004
                     /locus_tag="IYF54_000314"
     CDS             72273..73004
                     /locus_tag="IYF54_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9830683.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            73149..75176
                     /locus_tag="IYF54_000315"
     CDS             73149..75176
                     /locus_tag="IYF54_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ9830684.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            75284..76381
                     /locus_tag="IYF54_000316"
     CDS             75284..76381
                     /locus_tag="IYF54_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ9830685.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            76688..>77135
                     /locus_tag="IYF54_000317"
     CDS             76688..>77135
                     /locus_tag="IYF54_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9830686.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        25206 a        13900 c        16619 g        21410 t
ORIGIN      
        1 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
       61 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
      121 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
      181 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
      241 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
      301 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
      361 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
      421 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
      481 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
      541 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
      601 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
      661 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
      721 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
      781 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
      841 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
      901 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
      961 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
     1021 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
     1081 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
     1141 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
     1201 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
     1261 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
     1321 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
     1381 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
     1441 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
     1501 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
     1561 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
     1621 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
     1681 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
     1741 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
     1801 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
     1861 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
     1921 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
     1981 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
     2041 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
     2101 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
     2161 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
     2221 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
     2281 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
     2341 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
     2401 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
     2461 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
     2521 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
     2581 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
     2641 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
     2701 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
     2761 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
     2821 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
     2881 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
     2941 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
     3001 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
     3061 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
     3121 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
     3181 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
     3241 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
     3301 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
     3361 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
     3421 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
     3481 ttcaaaaaga aataatagga acagagtcgt tgttttttac gataaaataa ataagataca
     3541 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
     3601 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
     3661 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
     3721 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
     3781 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
     3841 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
     3901 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
     3961 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
     4021 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
     4081 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
     4141 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
     4201 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
     4261 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
     4321 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
     4381 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
     4441 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
     4501 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
     4561 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
     4621 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
     4681 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
     4741 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
     4801 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
     4861 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
     4921 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
     4981 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
     5041 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
     5101 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
     5161 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
     5221 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
     5281 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
     5341 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
     5401 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
     5461 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
     5521 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
     5581 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
     5641 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
     5701 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
     5761 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
     5821 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
     5881 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
     5941 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
     6001 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
     6061 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
     6121 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
     6181 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
     6241 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
     6301 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
     6361 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
     6421 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
     6481 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
     6541 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
     6601 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
     6661 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
     6721 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
     6781 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
     6841 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
     6901 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
     6961 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
     7021 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
     7081 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
     7141 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
     7201 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
     7261 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
     7321 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
     7381 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
     7441 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
     7501 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
     7561 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
     7621 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
     7681 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
     7741 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
     7801 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
     7861 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
     7921 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
     7981 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
     8041 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
     8101 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
     8161 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
     8221 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
     8281 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
     8341 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
     8401 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
     8461 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
     8521 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
     8581 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
     8641 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
     8701 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
     8761 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
     8821 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
     8881 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
     8941 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
     9001 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
     9061 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
     9121 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
     9181 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
     9241 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
     9301 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
     9361 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
     9421 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
     9481 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
     9541 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
     9601 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
     9661 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
     9721 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
     9781 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
     9841 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
     9901 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
     9961 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    10021 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    10081 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    10141 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    10201 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    10261 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    10321 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    10381 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    10441 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    10501 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    10561 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    10621 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    10681 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    10741 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    10801 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    10861 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    10921 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    10981 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    11041 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    11101 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    11161 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    11221 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    11281 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    11341 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    11401 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    11461 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    11521 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    11581 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    11641 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    11701 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    11761 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    11821 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    11881 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    11941 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    12001 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    12061 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    12121 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    12181 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    12241 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    12301 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    12361 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    12421 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    12481 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    12541 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    12601 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    12661 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    12721 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    12781 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    12841 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    12901 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    12961 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    13021 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    13081 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    13141 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    13201 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    13261 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    13321 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    13381 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    13441 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    13501 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    13561 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    13621 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    13681 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    13741 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    13801 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    13861 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    13921 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    13981 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    14041 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    14101 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    14161 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    14221 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    14281 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    14341 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    14401 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    14461 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    14521 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    14581 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    14641 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    14701 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    14761 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    14821 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    14881 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    14941 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    15001 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    15061 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    15121 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    15181 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    15241 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    15301 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    15361 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    15421 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    15481 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    15541 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    15601 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    15661 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    15721 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    15781 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    15841 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    15901 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    15961 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    16021 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    16081 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    16141 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    16201 tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc
    16261 ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa
    16321 caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga
    16381 tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag
    16441 cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt
    16501 tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac
    16561 taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag
    16621 atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga
    16681 tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga
    16741 aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat
    16801 ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt
    16861 ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc
    16921 aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga
    16981 ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta
    17041 ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg
    17101 gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga
    17161 cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa
    17221 gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat
    17281 cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt
    17341 tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat
    17401 cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc
    17461 ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac
    17521 cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc
    17581 cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt
    17641 ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt
    17701 tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa
    17761 aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta
    17821 tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg
    17881 cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac
    17941 gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc
    18001 tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt
    18061 taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg
    18121 tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc
    18181 aaacttaatt gaagttactg cttatatccc agggattggt cataacttac aagaacacag
    18241 tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt
    18301 tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg
    18361 aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata
    18421 tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt
    18481 cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc
    18541 ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat
    18601 gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca
    18661 atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac
    18721 caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta
    18781 aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg
    18841 gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct
    18901 gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt
    18961 tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa
    19021 acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc
    19081 cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc
    19141 cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa
    19201 gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc
    19261 aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt
    19321 gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa
    19381 acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg
    19441 gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca
    19501 aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac
    19561 cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca
    19621 gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct
    19681 gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa
    19741 gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta
    19801 tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac
    19861 ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa
    19921 gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact
    19981 gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt
    20041 tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg
    20101 catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt
    20161 ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt
    20221 gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct
    20281 gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt
    20341 gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta
    20401 gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct
    20461 caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt
    20521 cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa
    20581 ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt
    20641 ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca
    20701 ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa
    20761 actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca
    20821 gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat
    20881 gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc
    20941 ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact
    21001 cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat
    21061 gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt
    21121 cttaaaaccg gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt
    21181 tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc
    21241 tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa
    21301 aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt
    21361 aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct
    21421 atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca
    21481 acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga
    21541 cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg
    21601 gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt
    21661 caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa
    21721 gaattactag aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct
    21781 ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca
    21841 tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa
    21901 cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt
    21961 ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa
    22021 gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt
    22081 aaattgttag actacgctga agctggagac aacattggtg ctttactacg tggtgttgca
    22141 cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca
    22201 aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc
    22261 ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa
    22321 ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta
    22381 atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca
    22441 gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc
    22501 aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt
    22561 tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa
    22621 taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa
    22681 aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa
    22741 ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc
    22801 ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa
    22861 aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta
    22921 tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg
    22981 agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg
    23041 tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct
    23101 tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc
    23161 atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact
    23221 catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg
    23281 tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac
    23341 aatggaaacg gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt
    23401 atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat
    23461 tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga
    23521 tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca
    23581 aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca
    23641 tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct
    23701 tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga
    23761 tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat
    23821 tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat
    23881 gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga
    23941 agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg
    24001 tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg
    24061 acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc
    24121 acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact
    24181 tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa
    24241 caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca
    24301 agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga
    24361 ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt
    24421 gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca
    24481 aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact
    24541 gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca
    24601 aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg
    24661 aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa
    24721 aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa
    24781 gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta
    24841 aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa
    24901 tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat gctggacgta
    24961 acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg
    25021 tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg
    25081 atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca
    25141 tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata
    25201 tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca
    25261 ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag
    25321 tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga
    25381 tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca
    25441 acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg
    25501 taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta
    25561 aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag
    25621 ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg
    25681 tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg
    25741 atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca
    25801 aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt
    25861 atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag
    25921 gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac
    25981 gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact
    26041 gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt
    26101 aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc
    26161 attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt
    26221 gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt
    26281 cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc
    26341 gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg
    26401 tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt
    26461 cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt
    26521 atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa
    26581 accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa
    26641 caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga
    26701 tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg
    26761 cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca
    26821 agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac
    26881 tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac
    26941 atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa
    27001 aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt
    27061 gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa
    27121 tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt
    27181 atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac
    27241 aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa
    27301 ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa
    27361 gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc
    27421 gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac
    27481 cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt
    27541 ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat
    27601 tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa
    27661 gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa
    27721 aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa
    27781 acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg
    27841 cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt
    27901 ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa
    27961 tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc
    28021 tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa
    28081 aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt
    28141 tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg
    28201 tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa
    28261 aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt
    28321 catgaagatc gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag
    28381 gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga
    28441 caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga
    28501 aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat
    28561 tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt
    28621 agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac
    28681 cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct
    28741 aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc
    28801 tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt
    28861 atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa
    28921 accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc
    28981 aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt
    29041 ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg
    29101 tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga
    29161 tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga
    29221 agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc
    29281 gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac
    29341 acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca
    29401 tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa
    29461 agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca
    29521 gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct
    29581 ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt
    29641 ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta
    29701 aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt
    29761 ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca
    29821 atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc
    29881 ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt
    29941 gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg
    30001 aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat
    30061 caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat
    30121 gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga
    30181 aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc
    30241 aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt
    30301 aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt
    30361 tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg
    30421 tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt
    30481 tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca
    30541 aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga
    30601 aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca
    30661 aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc
    30721 cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag
    30781 cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa
    30841 cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta
    30901 aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg
    30961 aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta
    31021 tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa
    31081 agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa
    31141 cggacacgta gggtttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc
    31201 aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca
    31261 cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc
    31321 tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat
    31381 cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg
    31441 tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga
    31501 attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt
    31561 attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt
    31621 aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct
    31681 cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg
    31741 tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa
    31801 ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg
    31861 acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt
    31921 gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat
    31981 taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt
    32041 tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact
    32101 aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga
    32161 agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa
    32221 gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta
    32281 ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg
    32341 ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc
    32401 tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac
    32461 atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat
    32521 caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg tatcactgct
    32581 ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc aacttacgta
    32641 acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg agaacagatc
    32701 acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat catttctaga
    32761 ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga tccttcagac
    32821 atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt gatcgtgaca
    32881 ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac gaaacgcgta
    32941 gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg agttattcca
    33001 gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc attgagcggt
    33061 agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta taatacagca
    33121 cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta cgcctttgtt
    33181 caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta tataccaagc
    33241 gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg tttaagtaca
    33301 gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca aatggtttgg
    33361 gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat cggtgtagct
    33421 ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt tggattcatt
    33481 gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt tattaggagg
    33541 gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa cacaagctga
    33601 aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt tccgcgcagc
    33661 gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata aaggtgcatt
    33721 agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac cagatacaga
    33781 gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag ctctggacgc
    33841 aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg ttggtgaaga
    33901 aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag caacttacca
    33961 taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt
    34021 ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta tcaacatcaa
    34081 aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca ctattgatgg
    34141 tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa aatagcacga
    34201 ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa attatgatag
    34261 aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga attttgaggt
    34321 gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac
    34381 tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa actttgccga
    34441 atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact gtttcaggta
    34501 aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt gaattgtctc
    34561 catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac tccgtaaaac
    34621 tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa
    34681 agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa aacataaaca
    34741 acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt agatattcct
    34801 cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa cactactgct
    34861 aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga attaacgaat
    34921 gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg ggatcttcgt
    34981 cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg tggaatccgt
    35041 catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa
    35101 ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg aggttaaaac
    35161 ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt
    35221 attgcacata tccattctac attcaataac actatcatca tgatcactga tactcacgga
    35281 aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa aaaatcaact
    35341 ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga acacggatta
    35401 aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc aattcgttca
    35461 ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt tcctcataat
    35521 ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc
    35581 agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa gaatgattga
    35641 gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca agttcatcgt
    35701 tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc gtattttatt
    35761 atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac tgcatgaatt
    35821 ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca ttaaaggctt
    35881 agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta ccggacctgc
    35941 tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta acaaagatat
    36001 gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag tgaaacctgg
    36061 acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg gcgttcttcc
    36121 agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga acacacgtgt
    36181 tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg gttcaattga
    36241 accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg atatttttgt
    36301 gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa
    36361 agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct cttacaattg
    36421 tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg agcctgaaat
    36481 gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa aattgcacga
    36541 cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg aaaccaacgt
    36601 gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac
    36661 aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga aagaagttcg
    36721 ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg cacgtcgtca
    36781 agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt
    36841 cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg cagaacgtca
    36901 aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg caccaatggt
    36961 tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga ttcttttaga
    37021 gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc tcaagcgaat
    37081 atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa ttacctgtca
    37141 tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt gaaaattttg
    37201 tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc atggagccaa
    37261 ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc cccgcattga
    37321 aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga cacaatggtt
    37381 caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa tcagggatcg
    37441 tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga cagatggttg
    37501 gaatgatttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa gatgacgttg
    37561 cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt gtgaaagatg
    37621 cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt ttatcaggag
    37681 ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat atcattattt
    37741 tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc tctacgatca
    37801 agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat attgatgaag
    37861 cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt gaaggtacac
    37921 caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat ctaccatttc
    37981 ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt gaatatatga
    38041 cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag acttcactta
    38101 tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga cgatcaaaga
    38161 aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct tacttcagca
    38221 tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac gagatattca
    38281 gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca tcgtctttca
    38341 gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa
    38401 aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat tagaacaagt
    38461 tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg
    38521 tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat tggatgaacc
    38581 aacggcagga cttgatcccc agggcagaaa agaaatgatg gagatgtttt ggcgtcttca
    38641 taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg tagccaattt
    38701 tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga
    38761 ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga
    38821 gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt tgacagctga
    38881 agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg aataaattga
    38941 tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc cgagctaaat
    39001 tattagccag tttttatttt atcggaatca tttttttagc aaataattgg gaaacgtacc
    39061 ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat ttgaaatttt
    39121 ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct ttgcaggtct
    39181 tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc actgaatttg
    39241 gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt atgtcgactc
    39301 tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac ttgcttcgtc
    39361 cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg attgctcttc
    39421 gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg
    39481 gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt cctttgctga
    39541 ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct atggaagcaa
    39601 gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg cataatagag
    39661 acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt ttaagaggat
    39721 aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc
    39781 aagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc
    39841 ttttatctga aaagaaagaa cgaaaaaatt ttatagaaaa atcattattc cagataacct
    39901 tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc gtacgacgga
    39961 acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa
    40021 aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca
    40081 gatgccggag tacatgcgat cgggcaagta atccattttg attatccaga ggagcgtccg
    40141 ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa
    40201 gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa
    40261 tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt
    40321 ccttacccac ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac
    40381 gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaaatacg tacgatctac
    40441 gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat
    40501 ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc
    40561 cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga
    40621 cctacagcac accctgaagg actttatttg tatgaagtga aatatgaata agaggttatg
    40681 atagaagtgg tcagcttcaa gaaataagaa ggtatttacg aaaattactt ctcaaaattt
    40741 tgcaaaattt cggcttattt tcgaagaagc tacttctgga acaccgttta agaaagaggt
    40801 cgtgagcctg tcatttaact ctgagtcaca aagagcaatt tcaaaaaaca gcttctcatc
    40861 tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc
    40921 tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgcccttg gtgcggacag
    40981 tctgaacagg ttccatcgtt tctttatttt ctacgtcttc ttctgtgtgt gtttcgtggt
    41041 tgttcttcta ttgctggatc aatcgtgcca taaaaggggc gattgcggtc aaggtaccag
    41101 acataaagaa taaacaatgc aatacatagg acaaaaagga ttgcacctat cgtaaatcct
    41161 tcaggcaagt aactgaaagt gacagtatgt ttacctgctg gaacttttat catcaagaat
    41221 gcgtctttga aattgctgac cttgactggt ttcccatcaa ttttagcttt ccatcctttg
    41281 tcataaggaa tagttgtcac caacatggaa tcttcagcgg tatttacagt cccagaagct
    41341 tttctgccag tggcttccat ttcgacacct ttttctttga tctgattcat tgcggcttga
    41401 taggctacaa catctagacc aacgacttta ggctccatga aacttatttc tttcgtccca
    41461 taaaaacttg cagtaaatgt cactgttgtc tcttgaggat aatagccaag atcatagtat
    41521 tgaccagaaa tgttgatttg cgtttttcgc gatgtaccat tgacagtgat cgtcgcacta
    41581 gaactttcta attgcgcaaa attggttggg aacaaactta agtaagcttg tgtatttgca
    41641 ggcacatgga cttcccaagt gagctctttt ggctggttat ctgctgtttc tgagtaagtg
    41701 acgccagtcg ttgtctttgt gatcgtcgta ttcaactgat tcttcaaagt aggtgtatag
    41761 aatgtaaaat actgttcatt tagaccagac aatgtattga tcaagtttgt ctgactggtc
    41821 aaattatctg tcactggctg ctcaacatca ttaacggaca acggtgcagt aaatgctagt
    41881 ggcaatgcat tgacattctg atacagtgta tagtcgccgc tttgaccagc ttggcggaag
    41941 ccatatttac tgaacgtgct ttcgttttta gaaatgttat atttgattcc tgtaaaccca
    42001 tccatcagca aagtattatt gttataacgg atattcaaat ttgtccctct tgaacggaaa
    42061 cctaatttat ccaggtaaga ggaagaatta cgatttctta tagaagaaaa caaactgata
    42121 ccactgtaac catagttgat cgaatcgttt gcagatactg gatccagatt ttccaagcgg
    42181 taaaacgagt cggaatcttt ttttgtttca tttacaagag ctttcaagtc aggataagga
    42241 tcagtatata aactgcggga agcataattc caatcatcaa gaatgccatg gatcatactg
    42301 ttcgtattga ccagtgcttc agcagacatg atcaataata acaatatact cagatgattg
    42361 aatgaaagtt ttttatgctg ataaaatccc acagcaaaag cataaatccc taagaaaacc
    42421 actgtcaata caaaggaagt catcgtcaca taggtgtagc ttccttcagg tttgatgccc
    42481 catgccaccg caaagagagc agctacgacc aaaatgattc ctaacaacag cccaccatcg
    42541 tcttgttcta tcttttccca tccttttcct gcaagcagga gcactaagaa agagaacaga
    42601 taggcataac ggaataaaaa catattcggt gcatgcatac catgccagaa caaattcaat
    42661 ggaacaagat aaaaacttgc gataagtatg gcgaaaagac tgccgtacaa aaatttattt
    42721 ttcaacggta ctttccgact ggcaaaataa aacagacaga ggactaatgg tagcagacct
    42781 gcgtaaacga acggaatcga tccgtatttt gtcgtgtcat aaacaccaat catatttttc
    42841 atgattagat ccagataaga agtcgcttct gttttcaacg tagtgatctg actcagtgcc
    42901 tcgccatttg accgaagatc cagcactgct ggcaatacaa tgatcattga agcgccgcct
    42961 gccagtaaag aagtgatacc ataaggaaca atcgtttttt tatattctga ccagtttgtc
    43021 aatagacggg cgataaaata taagaaagaa aatacgccaa tcataaaacc catataaaaa
    43081 ctggataaaa acaagagtaa ataactgata aataaaagtt tcggccgttt aagatccata
    43141 acacgatgaa tccccaaaat cactaatgga agataaacaa atgcatccag ccacatgatg
    43201 atctccgaat gtgcagtgat aaatgagatc aatgcgtaac tgacgctaag tgcaacatgc
    43261 ccccagcgag gaatcttgaa cgtacgtgca agaaaccaaa aagccaatcc ggcacagccg
    43321 accttaagca aagtcagaac atacagtgca tctggcatca tctggtttgg aaaaaagaac
    43381 accagtggcg tgaagatccc gcctagatag taagaaatta aagataaata attcaatcct
    43441 agagaagcat tccatgtata aaaaatactt tgctttccaa gaagaacatt tcggaagctg
    43501 gcatgaaaat tagaaaactg tgaaaaggca tcacttgcca aaatacttct gctgcttcca
    43561 ggatagattc cgatacttag ataaacaagt gctaaaatca aaaacggaat caggaaacta
    43621 gctgctgcgt agcgtccgtt ttctctaaag aatgacttta aatttttttt catatttatt
    43681 ttccaccaaa tttctatgaa gttgtattca gtttatcata tccttgtttt tctctgctta
    43741 aaaagagtct agttttcaca aaaaaataaa atttagaata aataacttca taatttgttc
    43801 gtataacaaa aataaacgca aaaaaagggt aggaatcaaa agtcccaccc ttaaaaattt
    43861 ttattaaata aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt
    43921 tcaacttttc ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg
    43981 accataacgc gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg
    44041 tgtgaacggt tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg
    44101 gtaatttcct cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa
    44161 catatcatat agaatgggaa tttgcaagta cattttcata gatacaattg acaattgaga
    44221 agaataattg taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt
    44281 gggtaactgc cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga
    44341 gttttttacc cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac
    44401 cattgaaatt accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt
    44461 cggtgttgta ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg
    44521 taaagaaaat tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga
    44581 tgtttatacg attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga
    44641 aaaagtgaaa tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt
    44701 tgtacaaggt gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc
    44761 gatctgaagg aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga
    44821 aatggtccag gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa
    44881 tgtattccct gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg
    44941 agctaaggca gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc
    45001 aaaaggattg ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg
    45061 tggattttct aagcaaattc cagatgttgt ctcattaaat gcttctgatc ttgctgcagc
    45121 cttcactcat ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat
    45181 tttgacagta gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga
    45241 tgtcgttgaa gtaatgacag ctgttgtaaa aggagcaaag cgtgctttag ctaaaactcc
    45301 agacttgctt ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct
    45361 gtttgtttat gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata
    45421 tgaaccttct ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca
    45481 aggtcaaatg gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat
    45541 cggagaagga cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga
    45601 tggtatagga gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca
    45661 tacagaaaac ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat
    45721 caaagtcgac aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa
    45781 cgagtttgcg aaaaaagaaa taccacgtat cccgtatggt atcgtagcga tcgctgctgg
    45841 taaaggcgtc caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtggaca
    45901 aacaatgaat ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa
    45961 agtgattgtc ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt
    46021 agcagatatc ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat
    46081 gctttcattc aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt
    46141 gtctagcgtt gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt
    46201 agagatcaaa gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc
    46261 agatatcaca gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat
    46321 cgtaacgatc ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa
    46381 actaacagaa aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta
    46441 tccttatcta ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc
    46501 attcataaaa ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt
    46561 taaaggaggg aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga
    46621 gtcggagaaa aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta
    46681 ctgacttatt atccttttcg ctacgaagat attcaggaaa gaaatctaaa cgagatccag
    46741 gatcaagaaa aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt
    46801 ggttataaaa aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt
    46861 tcttttttta atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta
    46921 tacggtaaat gggatgcaaa gcgtaaagcc ttgaacggga tgaagatttt aggttctcaa
    46981 acaatggatg acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta
    47041 gttgatctaa tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca
    47101 aacaaccttg tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat
    47161 tttccaaaag accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc
    47221 tttttatttc aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt
    47281 ttggagatcc tttatgatgt ccaaaaattg aaacaattta ctcaaaagct accttttgaa
    47341 ctgacaaatg cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag
    47401 catatgcagc gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt
    47461 gcgttatacg ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc
    47521 ttggcacaac aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc
    47581 gcattgatta cggggtcaac gaaagcaaaa gaacgacgtg agctccttga aaaactagag
    47641 caaggggaaa ttaacgtcgt gatcggcaca catgcattga tccaagatgg agtcgaattt
    47701 cagaatttag gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa
    47761 gtacttcgtg aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct
    47821 agaacattag ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca
    47881 gctggacgta tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg
    47941 gattggacga aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt
    48001 gaagaatcag aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa
    48061 ctatttgcac caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa
    48121 gaagtaatca tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta
    48181 attgaagtag gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga
    48241 tttggattgg cacagctgca ccaacttcgt ggacgtgttg gacgcggaag tgatgcttcc
    48301 tactgtatct tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg
    48361 acagagacca ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt
    48421 gaagtatttg gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg
    48481 gatggaaatg ttttggaggt agccagagaa gaggctgcag ccctttggca aataaaggat
    48541 tggcagatgc ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat
    48601 aatcgttatt tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg
    48661 gaaacaatga aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta
    48721 gaaggcgtgg ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa
    48781 gaagcagaaa tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac
    48841 gaaaaaatca atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca
    48901 atggtccttg ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg
    48961 aatacaggcg cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa
    49021 cgccctggct tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg
    49081 ctggatcttg gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta
    49141 ggttcttttt atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac
    49201 aacggaacag aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag
    49261 aatgaaacaa gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg
    49321 gctgatgtag tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga
    49381 acagcaatga atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga
    49441 aaaatgggcg ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat
    49501 tccaaacatg gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga
    49561 gcaacaggcc ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca
    49621 gatgtagttg gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc
    49681 tgaagcgatc cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta
    49741 gccatggtgt aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga
    49801 agaagtattt aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa
    49861 agtgacggat gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga
    49921 attcgtttta gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca
    49981 aattgaaaca gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat
    50041 aaaagcaaag aaggataaaa agctgcatgc agctttttat ccttcttttt ttaatctttt
    50101 tgacacgtaa aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag
    50161 taaagcggtt ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg
    50221 ttataaaacg atttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga
    50281 agcagagagg ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac
    50341 aggattccga ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga
    50401 caaaaacgag attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac
    50461 tacgattatg ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa
    50521 cgatctgaat ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat
    50581 cggaatgatt ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca
    50641 gatcaaagaa ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc
    50701 actagaattg ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc
    50761 ccatgaacta tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa
    50821 acctccaatc gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca
    50881 gattcttgat ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac
    50941 tcatgactta ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca
    51001 gttcgttgaa gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg
    51061 ctctttactg aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga
    51121 aggtgtcgtt ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat
    51181 tccatggata ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa
    51241 ccacttggta cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt
    51301 gtagatgaca gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg
    51361 attcttcaat cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga
    51421 aaaaggaaag acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa
    51481 agccgttgtg ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt
    51541 aacgaagcga agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca
    51601 agattcaatg tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat
    51661 ccgaaatttt gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga
    51721 tatcgttggg atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca
    51781 acgtcaacgt ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga
    51841 tgaacctgta tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg
    51901 aatccagcaa gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa
    51961 acacatgtgc gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag
    52021 agaagacatt tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc
    52081 gcaaatcgat gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga
    52141 gtatatccaa aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac
    52201 gatcacaact acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg
    52261 attctgcgac gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc
    52321 ttattagccc aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat
    52381 ccggcagtga tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag
    52441 tacttccgtt ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa
    52501 cgctctgttg ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg
    52561 acagttgtcc ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa
    52621 aattcaatct tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta
    52681 tttatcttcg gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca
    52741 agtggatcac aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg
    52801 aaatatattc tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa
    52861 tatctgcgaa gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc
    52921 aaaggggtac cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg
    52981 atggcttctc aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa
    53041 aagatttttg gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac
    53101 tataccgtga tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta
    53161 ctttcagata tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca
    53221 gaaaggagaa tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc
    53281 ctatggggat acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat
    53341 taggtttgct agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag
    53401 acaaagtgat gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac
    53461 aaggacaaaa gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga
    53521 tcataggtgc tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca
    53581 tcggggttgg attagggatc atctctggct actacggagg gatcattgac aacatcttga
    53641 tgcggatcgt tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat
    53701 cgattatttc cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg
    53761 tagctaaagc ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca
    53821 gtgcctcaaa aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga
    53881 atctaagttc attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg
    53941 agacaacttt gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat
    54001 tgatcggtta tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg
    54061 catcaatcct tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc
    54121 gttctgcaga tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg
    54181 aagaaaacga tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg
    54241 ggcggaggta cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa
    54301 ttaacttttc caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa
    54361 gctgcggtcg caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat
    54421 tcatatgatg cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat
    54481 ttcagaatca cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg
    54541 acaatcaaag taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat
    54601 gtgattttcc cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac
    54661 actttccgta acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc
    54721 ggaatcaaaa aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc
    54781 atgcttcaat cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt
    54841 gtagcaatca aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc
    54901 ccttattaca tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac
    54961 tactggaatg gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt
    55021 gcaactgaag cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact
    55081 tacgatacct ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat
    55141 acttatctag gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat
    55201 ccagattcaa aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat
    55261 aatgcagtag gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct
    55321 ccagtattcc agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa
    55381 gcgaaaaaac tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa
    55441 gacaaagatg gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg
    55501 caaccattgg cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac
    55561 acaacaggtc gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct
    55621 gaaattgatg tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta
    55681 tggggaccaa atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg
    55741 atcaacgata tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa
    55801 aaatggcaac aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa
    55861 gtattgccag tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca
    55921 tgggcaaatg tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa
    55981 gggcgatgtc ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc
    56041 aaggcgctga tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt
    56101 aagtcaaatt tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg
    56161 aaactaaacg aatttttcac aacttctcgt caatcactag gattattaaa gctttactta
    56221 ccttttgacc gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa
    56281 aaatatccat aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa
    56341 actataacat cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct
    56401 atgtgaatga gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag
    56461 atgccgtttt agaactgatg gtttctcagt atttattccg tttatatcct catttgccag
    56521 aaggaaaatt gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg
    56581 ccaaagaatg tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag
    56641 gacgtacgcg accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc
    56701 ttgaccaggg attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca
    56761 aagcaggtgc tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa
    56821 aatcaggtga tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac
    56881 gagtattttg gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat
    56941 cgaaaaaatt agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag
    57001 tgagggaaag ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac
    57061 aagacagtca ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga
    57121 aaaagcaata ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg
    57181 cgaggcggaa aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat
    57241 attgcagaag tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat
    57301 gagatcagtg taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa
    57361 caaagctgca gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa
    57421 tcgttctcaa tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac
    57481 cgtcgtggga tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa
    57541 gcagaacaga agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac
    57601 gaattggaag aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta
    57661 cggctaaaag aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca
    57721 gcaaaaaaag attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc
    57781 aaactatctg agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat
    57841 gcgcaagccg atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa
    57901 ctgaagcaga cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa
    57961 agctcgcaag aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa
    58021 aagcttcaag aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct
    58081 gaagcaaatc tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa
    58141 ttattggcag aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga
    58201 aacgaactga agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg
    58261 ttagccaagc aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa
    58321 ttgacacaaa agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa
    58381 atgatccaac aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg
    58441 tatcaattaa tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc
    58501 caagaaaact attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag
    58561 ctttcgggta ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta
    58621 gctatcgaaa cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat
    58681 gctcggcaag ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca
    58741 ctgacgacaa tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta
    58801 gaagggttta tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg
    58861 gttcaaaacc tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc
    58921 gcgcaaacga tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc
    58981 ggaggatcca tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa
    59041 aatcaagaat tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca
    59101 caagaaaaaa aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa
    59161 gttttgcgta cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta
    59221 caaaacatca taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag
    59281 acacgtgaac tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac
    59341 aaacaaacag atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt
    59401 caagaaagcg accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa
    59461 caagcagatc ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt
    59521 gcccgagtcc aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg
    59581 acattgacag cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt
    59641 aacgaattat ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag
    59701 cgagatagaa ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt
    59761 gagcaaaaag cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa
    59821 atgattttag ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa
    59881 gcttcccaag actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc
    59941 ttaaaagaac agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa
    60001 caagtcagtg aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa
    60061 aaccaattgt ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa
    60121 gtatttgaag ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt
    60181 aaagctgaat tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa
    60241 gtacaacctc ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca
    60301 cttacagcca tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc
    60361 ttagatgagg tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg
    60421 agcgatttcc aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc
    60481 gcagcagatg tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct
    60541 gtacggatgg aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga
    60601 aaatgatcaa attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga
    60661 tttcagatga gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc
    60721 tatgtacagg acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag
    60781 atgaaggtga ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg
    60841 aaatcatgga aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc
    60901 agcaattgga tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg
    60961 ccccaaggaa gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg
    61021 atataagcga tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag
    61081 aagaattaga tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca
    61141 tcaaaacaag aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta
    61201 tttctttatt ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg
    61261 aggaaaatga tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg
    61321 tgcctttcgt caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag
    61381 ccaaagtgat ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa
    61441 atcaagcgtg cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac
    61501 gacaagggtt tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc
    61561 aattttcgaa cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca
    61621 gatgtaggtt ttgaaacagc gatacgaatt accgaagcat tacgtcaaga agtgaaactc
    61681 agaaatgcga aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc
    61741 tatgaagcgg aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca
    61801 gttattttgt ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc
    61861 catcagttca aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct
    61921 ggagcaatcg accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc
    61981 aatgcaggag gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag
    62041 caggcagatg ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg
    62101 aacgaattag aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa
    62161 acacttcttg tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc
    62221 aaagaaacaa cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc
    62281 ggtatcgtac ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt
    62341 gaaggaatcg atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa
    62401 ggattactaa aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag
    62461 ctccttgttt gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac
    62521 ttatttgtaa gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt
    62581 agattcatat gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc
    62641 aatgcgcgcc cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt
    62701 gatcttgcag gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat
    62761 cacccctata tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc
    62821 gattctattc ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct
    62881 ataccgaaag aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac
    62941 gaagagatca ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta
    63001 gcaaaagaat acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt
    63061 ggcgatgagt tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa
    63121 ttcaaaacag aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc
    63181 acttatttat tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat
    63241 ccgcaagaac ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat
    63301 gtgaaagttt cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa
    63361 gaagggaaac tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag
    63421 aaagtcgcag atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg
    63481 aatccttatg caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg
    63541 atgtacatgt tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg
    63601 actcgaaata acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa
    63661 ggtagagaaa ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct
    63721 ctttatttag tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg
    63781 aaactctatc aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat
    63841 caatattata aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa
    63901 ctttctactc agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta
    63961 gatgagtcgg atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat
    64021 gcaaacatga caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta
    64081 acgaaaaaag tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc
    64141 atggaagaag cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct
    64201 aaatcaacca attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat
    64261 tatcaagcag gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt
    64321 atgccgggaa cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg
    64381 cgagtccctg ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa
    64441 aaaaatagtg accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta
    64501 ggtgaattag aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa
    64561 gccaatcaaa ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc
    64621 gatatgactg atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca
    64681 ctaggagcta agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca
    64741 actgataaaa aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat
    64801 gtttatcctt ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat
    64861 cctgaagtat tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat
    64921 caaaagcttt ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc
    64981 agaagctttt tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt
    65041 attttttttc gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc
    65101 cgaattcctc aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt
    65161 tcgttgctag ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta
    65221 tggaagcatc tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga
    65281 tcatttcagc aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat
    65341 gctgttcttc agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta
    65401 catcatcttg tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag
    65461 catattccca ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac
    65521 ttaaaataag aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga
    65581 ctagtagcat tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta
    65641 caattgtcag aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt
    65701 ttgccacgaa cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc
    65761 atgcaacatt tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt
    65821 gcgcgtttag gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga
    65881 tccttgacag cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga
    65941 ttgttcctcc agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc
    66001 attggttcag ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca
    66061 tggcttcaag ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt
    66121 gttccattgt tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc
    66181 gaatcgtttg cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc
    66241 ttagtatcat tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga
    66301 gtgatcattc cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc
    66361 gtaactgctt tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg
    66421 atttttcctt atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc
    66481 ctttatcttc tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg
    66541 tatgcttgtt cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa
    66601 ctggcatttt gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct
    66661 agctgtcagc agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc
    66721 ttcagtattg ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat
    66781 cggaggagtg acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct
    66841 gatggtcggg ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct
    66901 ttttttatta ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt
    66961 tttacaagtc atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag
    67021 ctttggcaat atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg
    67081 gtacctgtta tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc
    67141 agtaaacctc gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct
    67201 tttgacaggg acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc
    67261 aaatatcagt tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt
    67321 tctggtagct atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg
    67381 gaatacaaca gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt
    67441 gattcgcgaa aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat
    67501 ggaaaatcag tggttaaaga tgtacagtta acgattcctg aaaagaaatt gactgctttt
    67561 atcggtccaa atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca
    67621 aaagatacag gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa
    67681 ttagccaaaa aattatcgac cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg
    67741 aaagaattag tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac
    67801 gaggaaatcg ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg
    67861 attgacactc tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa
    67921 gacacggatt atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt
    67981 caaatcatgc agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt
    68041 cttcatgata tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga
    68101 cagttattta cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta
    68161 tacgaaatgg acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa
    68221 aactaacatt aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc
    68281 atttggttta gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag
    68341 cagcacacaa actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt
    68401 gccaaataat ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc
    68461 tcttggtgtt ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc
    68521 atcctataaa aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa
    68581 tcagcttcag ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct
    68641 aagtcaaatt gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt
    68701 ccagcaaaat gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca
    68761 attagaggaa ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa
    68821 aaaagcacta gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag
    68881 attcggtttc atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc
    68941 cactcacggt caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt
    69001 tgtagtagac agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa
    69061 tgaactggta gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga
    69121 tgtctggtat ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa
    69181 tcaagcattt aaatagcata aataacagag gattaaactc tcatactatg taacaataat
    69241 gtttcttaca ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg
    69301 taaaatcgta ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac
    69361 gtgaattcat tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt
    69421 gtgatccatt ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg
    69481 aaacttagat aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg
    69541 atgttccatc tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat
    69601 tttctgtcca gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac
    69661 gatgttccat ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta
    69721 ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa
    69781 caatgttcaa tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt
    69841 taggcatgtg tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg
    69901 gcgggaagct tactccaccg ttttaataat tgatagaaat aactctcttc tgctttgatt
    69961 ttatactcgc ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa
    70021 atatccaatg ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa
    70081 ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg
    70141 attcctccaa agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc
    70201 cgtgaacaaa aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta
    70261 ttttttgtta tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt
    70321 tttccgttca agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa
    70381 tcataataaa cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt
    70441 agttgcatct gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag
    70501 aagtgacaaa agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt
    70561 tattagatat cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg
    70621 atacggcatt tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt
    70681 ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca
    70741 tggatgatcc atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac
    70801 caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg
    70861 gaaaattggc gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc
    70921 tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag
    70981 atacggctaa taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat
    71041 tatctacgac atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga
    71101 tggaccattt tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga
    71161 ccgttctttt agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg
    71221 aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa
    71281 cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg
    71341 aagcaaaaaa acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca
    71401 tgtatcaatc tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat
    71461 cattcggaga attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc
    71521 atcgtcgctt tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt
    71581 attattatga atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct
    71641 atgatcctat gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc
    71701 aaacacaaca tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca
    71761 gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag
    71821 atccatttat cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg
    71881 atcggatgat ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga
    71941 taatcacaaa aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
    72001 gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag
    72061 gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc
    72121 aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt
    72181 tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga
    72241 tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag
    72301 cagcaatgat gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat
    72361 acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa
    72421 ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc
    72481 caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga
    72541 ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg
    72601 ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga
    72661 aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag
    72721 cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc
    72781 aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg
    72841 ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa
    72901 atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt
    72961 ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact
    73021 gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag
    73081 gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa
    73141 aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata
    73201 tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag
    73261 acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag
    73321 aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg
    73381 agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg
    73441 atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca
    73501 tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta
    73561 tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct tatttatact
    73621 taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta
    73681 gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt
    73741 ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata
    73801 aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg
    73861 gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg
    73921 gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat
    73981 gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga
    74041 ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt
    74101 caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca
    74161 catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag
    74221 gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata
    74281 agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag catatcttga
    74341 tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa
    74401 atctgacaga ttattttatt gctggagata cgttaataat cgaattggag acaactttgc
    74461 aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt
    74521 tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc
    74581 atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg
    74641 ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact
    74701 cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt
    74761 acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata
    74821 aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag
    74881 atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg
    74941 gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag
    75001 ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc
    75061 ttttagattc tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa
    75121 tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg
    75181 aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt
    75241 gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat
    75301 tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag gggcactttt
    75361 taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga
    75421 gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt
    75481 agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt
    75541 gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa
    75601 agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct
    75661 aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga
    75721 tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga
    75781 aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat
    75841 gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca
    75901 aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa
    75961 tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga
    76021 ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa
    76081 tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa
    76141 tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg
    76201 ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga
    76261 tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt
    76321 gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta
    76381 aggttaagat aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa
    76441 caatataaaa tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa
    76501 taacaggttt gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga
    76561 caggacgatg tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa
    76621 tcagttttat ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg
    76681 atgactaatg aattatgagt tttttttaag caaagaagat cacagaagtt attcattact
    76741 taaatattta gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt
    76801 gtcgctatcg acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa
    76861 gttcgatcta gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat
    76921 taatggaaaa tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat
    76981 gaaaatggta ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca
    77041 gcatcacgtg agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa
    77101 aaaatatcaa attttttttg aaaagagaaa gttga
//
LOCUS       DACIWG010000005        74001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.005, whole genome shotgun sequence.
ACCESSION   DACIWG010000005 DACIWG010000000
VERSION     DACIWG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.005"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1175)
                     /locus_tag="IYF54_000318"
     CDS             complement(<1..1175)
                     /locus_tag="IYF54_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9830687.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNE"
     gene            1658..1873
                     /locus_tag="IYF54_000319"
     CDS             1658..1873
                     /locus_tag="IYF54_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ9830688.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(2114..2300)
                     /locus_tag="IYF54_000320"
                     /pseudo
     CDS             complement(2114..2300)
                     /locus_tag="IYF54_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            2734..3693
                     /locus_tag="IYF54_000321"
                     /pseudo
     CDS             2734..3693
                     /locus_tag="IYF54_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            4224..4634
                     /locus_tag="IYF54_000322"
     CDS             4224..4634
                     /locus_tag="IYF54_000322"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ9830689.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            4627..5082
                     /locus_tag="IYF54_000323"
     CDS             4627..5082
                     /locus_tag="IYF54_000323"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9830690.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            5099..5830
                     /locus_tag="IYF54_000324"
     CDS             5099..5830
                     /locus_tag="IYF54_000324"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9830691.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            5847..6659
                     /locus_tag="IYF54_000325"
     CDS             5847..6659
                     /locus_tag="IYF54_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9830692.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(6875..8158)
                     /locus_tag="IYF54_000326"
                     /pseudo
     CDS             complement(6875..8158)
                     /locus_tag="IYF54_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8233..9644)
                     /locus_tag="IYF54_000327"
     CDS             complement(join(8233..9089,9089..9644))
                     /locus_tag="IYF54_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9830693.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            complement(9733..9816)
                     /locus_tag="IYF54_000328"
                     /pseudo
     CDS             complement(9733..9816)
                     /locus_tag="IYF54_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10333..11583
                     /locus_tag="IYF54_000329"
                     /pseudo
     CDS             10333..11583
                     /locus_tag="IYF54_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(12157..13011)
                     /locus_tag="IYF54_000330"
     CDS             complement(12157..13011)
                     /locus_tag="IYF54_000330"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830694.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(13028..14026)
                     /locus_tag="IYF54_000331"
     CDS             complement(13028..14026)
                     /locus_tag="IYF54_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9830695.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            complement(14061..14885)
                     /locus_tag="IYF54_000332"
     CDS             complement(14061..14885)
                     /locus_tag="IYF54_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9830696.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            complement(14875..15657)
                     /locus_tag="IYF54_000333"
     CDS             complement(14875..15657)
                     /locus_tag="IYF54_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9830697.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            complement(15681..16151)
                     /locus_tag="IYF54_000334"
     CDS             complement(15681..16151)
                     /locus_tag="IYF54_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9830698.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            complement(16169..16591)
                     /locus_tag="IYF54_000335"
     CDS             complement(16169..16591)
                     /locus_tag="IYF54_000335"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAQ9830699.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            complement(16719..19442)
                     /locus_tag="IYF54_000336"
     CDS             complement(16719..19442)
                     /locus_tag="IYF54_000336"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9830700.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            19632..20648
                     /locus_tag="IYF54_000337"
     CDS             19632..20648
                     /locus_tag="IYF54_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9830701.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            20756..21625
                     /locus_tag="IYF54_000338"
     CDS             20756..21625
                     /locus_tag="IYF54_000338"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830702.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            21640..22344
                     /locus_tag="IYF54_000339"
     CDS             21640..22344
                     /locus_tag="IYF54_000339"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9830703.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            23565..24178
                     /locus_tag="IYF54_000340"
                     /pseudo
     CDS             23565..24178
                     /locus_tag="IYF54_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(24927..28076)
                     /locus_tag="IYF54_000341"
     CDS             complement(24927..28076)
                     /locus_tag="IYF54_000341"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ9830704.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(28089..29306)
                     /locus_tag="IYF54_000342"
     CDS             complement(28089..29306)
                     /locus_tag="IYF54_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ9830705.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(29296..30891)
                     /locus_tag="IYF54_000343"
     CDS             complement(29296..30891)
                     /locus_tag="IYF54_000343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ9830706.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(31401..32183)
                     /locus_tag="IYF54_000344"
     CDS             complement(31401..32183)
                     /locus_tag="IYF54_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830707.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(32248..33084)
                     /locus_tag="IYF54_000345"
     CDS             complement(32248..33084)
                     /locus_tag="IYF54_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9830708.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(33102..34511)
                     /locus_tag="IYF54_000346"
     CDS             complement(33102..34511)
                     /locus_tag="IYF54_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9830709.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(34525..36348)
                     /locus_tag="IYF54_000347"
     CDS             complement(34525..36348)
                     /locus_tag="IYF54_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830710.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(37191..37517)
                     /locus_tag="IYF54_000348"
     CDS             complement(37191..37517)
                     /locus_tag="IYF54_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830711.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(37563..37925)
                     /locus_tag="IYF54_000349"
     CDS             complement(37563..37925)
                     /locus_tag="IYF54_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ9830712.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(37962..38177)
                     /locus_tag="IYF54_000350"
     CDS             complement(37962..38177)
                     /locus_tag="IYF54_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9830713.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            38304..38522
                     /locus_tag="IYF54_000351"
     CDS             38304..38522
                     /locus_tag="IYF54_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9830714.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVEL"
     gene            complement(38716..40713)
                     /locus_tag="IYF54_000352"
     CDS             complement(38716..40713)
                     /locus_tag="IYF54_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830715.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(40811..41086)
                     /locus_tag="IYF54_000353"
     CDS             complement(40811..41086)
                     /locus_tag="IYF54_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9830716.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            41318..42052
                     /locus_tag="IYF54_000354"
     CDS             41318..42052
                     /locus_tag="IYF54_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9830717.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            42091..43233
                     /locus_tag="IYF54_000355"
     CDS             42091..43233
                     /locus_tag="IYF54_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9830718.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(43328..44719)
                     /gene="sufB"
                     /locus_tag="IYF54_000356"
     CDS             complement(43328..44719)
                     /gene="sufB"
                     /locus_tag="IYF54_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ9830719.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(44724..45194)
                     /locus_tag="IYF54_000357"
     CDS             complement(44724..45194)
                     /locus_tag="IYF54_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ9830720.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(45181..46416)
                     /locus_tag="IYF54_000358"
     CDS             complement(45181..46416)
                     /locus_tag="IYF54_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9830721.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(46413..47699)
                     /gene="sufD"
                     /locus_tag="IYF54_000359"
     CDS             complement(46413..47699)
                     /gene="sufD"
                     /locus_tag="IYF54_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ9830722.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(47712..48485)
                     /gene="sufC"
                     /locus_tag="IYF54_000360"
     CDS             complement(47712..48485)
                     /gene="sufC"
                     /locus_tag="IYF54_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ9830723.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(48682..49530)
                     /locus_tag="IYF54_000361"
     CDS             complement(48682..49530)
                     /locus_tag="IYF54_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9830724.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(49554..50240)
                     /locus_tag="IYF54_000362"
     CDS             complement(49554..50240)
                     /locus_tag="IYF54_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830725.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(50233..51267)
                     /locus_tag="IYF54_000363"
     CDS             complement(50233..51267)
                     /locus_tag="IYF54_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9830726.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(51662..52000)
                     /locus_tag="IYF54_000364"
     CDS             complement(51662..52000)
                     /locus_tag="IYF54_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ9830727.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(52002..52358)
                     /locus_tag="IYF54_000365"
     CDS             complement(52002..52358)
                     /locus_tag="IYF54_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ9830728.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(52364..53551)
                     /locus_tag="IYF54_000366"
     CDS             complement(52364..53551)
                     /locus_tag="IYF54_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9830729.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(53742..54587)
                     /locus_tag="IYF54_000367"
     CDS             complement(53742..54587)
                     /locus_tag="IYF54_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9830730.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            54821..55786
                     /locus_tag="IYF54_000368"
     CDS             54821..55786
                     /locus_tag="IYF54_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ9830731.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            56163..57206
                     /locus_tag="IYF54_000369"
     CDS             56163..57206
                     /locus_tag="IYF54_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ9830732.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(57268..57732)
                     /locus_tag="IYF54_000370"
     CDS             complement(57268..57732)
                     /locus_tag="IYF54_000370"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ9830733.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVFPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            57978..58925
                     /locus_tag="IYF54_000371"
     CDS             57978..58925
                     /locus_tag="IYF54_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9830734.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(59136..61208)
                     /locus_tag="IYF54_000372"
     CDS             complement(59136..61208)
                     /locus_tag="IYF54_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ9830735.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(61141..61518)
                     /locus_tag="IYF54_000373"
     CDS             complement(61141..61518)
                     /locus_tag="IYF54_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ9830736.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(61647..62207)
                     /locus_tag="IYF54_000374"
     CDS             complement(61647..62207)
                     /locus_tag="IYF54_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9830737.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            62357..63649
                     /locus_tag="IYF54_000375"
     CDS             62357..63649
                     /locus_tag="IYF54_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9830738.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(63709..64827)
                     /locus_tag="IYF54_000376"
     CDS             complement(63709..64827)
                     /locus_tag="IYF54_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ9830739.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(64841..66262)
                     /gene="arcD"
                     /locus_tag="IYF54_000377"
     CDS             complement(64841..66262)
                     /gene="arcD"
                     /locus_tag="IYF54_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ9830740.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(66508..67629)
                     /gene="mnmA"
                     /locus_tag="IYF54_000378"
     CDS             complement(66508..67629)
                     /gene="mnmA"
                     /locus_tag="IYF54_000378"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ9830741.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            68033..68632
                     /locus_tag="IYF54_000379"
     CDS             68033..68632
                     /locus_tag="IYF54_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ9830742.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(68770..70056)
                     /gene="efmA"
                     /locus_tag="IYF54_000380"
     CDS             complement(68770..70056)
                     /gene="efmA"
                     /locus_tag="IYF54_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ9830743.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(70327..70671)
                     /locus_tag="IYF54_000381"
     CDS             complement(70327..70671)
                     /locus_tag="IYF54_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ9830744.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(70808..71944)
                     /locus_tag="IYF54_000382"
     CDS             complement(70808..71944)
                     /locus_tag="IYF54_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9830745.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(72133..73107)
                     /locus_tag="IYF54_000383"
     CDS             complement(72133..73107)
                     /locus_tag="IYF54_000383"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ9830746.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(73187..73750)
                     /locus_tag="IYF54_000384"
     CDS             complement(73187..73750)
                     /locus_tag="IYF54_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830747.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
BASE COUNT        21980 a        15083 c        12558 g        24380 t
ORIGIN      
        1 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
       61 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
      121 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
      181 ccaatcgtta atggctcttc ccgtgttcga ctgtacacat ccgcacaact gattttaatg
      241 atattcttca gcttaccaat attcccacta aagggatgag ataacattag agacaaagct
      301 tcttttgaaa caataatatc tttttgtaaa atatccgctt cattttgaaa aaataattga
      361 atcagctcta accgttctcg aatgggacga tcttcaaaag ttggcaacat aacactgatt
      421 tgaatacgtc gatcaaaggt gtctaataaa taatcttcac ttttttccgt cgtggcgaag
      481 ataaaacgaa ccttagcaga atgccagttt acattttcac cgactggacg ataatttcca
      541 gtatcaataa aaacaaagag cttttcttga ttttctttcg acaaacgatg gacctcatca
      601 agaaaaagat agccattgtt ggctgttgcc aataacccct ctgtatcctt ctcagccccc
      661 gtaaaggccc ctttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
      721 taatcggcac aatttaaaat agcaaatggt gcatcttctt caataatttg ctcgtggata
      781 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cccccgtggc tccatttatt
      841 aatacactga gtccgttagg cggatatttg acagctgcaa tacatttttg aatgacctct
      901 ctcagacttc cgtcatgacc caccacagaa tgaaaggctt cgtttatttg ctccgtatta
      961 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     1021 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     1081 agattcttcg aaattttctc ggcagtgaca gaagacatat tccctgttag tcgtatcagc
     1141 tcatcaaaaa ctcgatcttt agcgctcttt ttcattttcc cctccagttc aacgtgtcta
     1201 gaagcataat aaactatcgg ctacaatatg tctattctat atttctgttc caattttact
     1261 ttgaaacaaa aataatcgag cttaaactga tattagttat aaaactatag ttagattacc
     1321 aatcttcctc tcatacaata aacttctctc acctgcatcc aacctactta tgtgaaagaa
     1381 taactgccga ttatttatct tttttacagt tactttcgaa cataaaaaaa tcaccttcaa
     1441 atactagaaa aactactatt tgatggtggt ttagaacaat ttccgttgat tcagtatcgt
     1501 ctggtcccac atcctctgtc tcattaatca caatagcata ctccaaagga taaagctggt
     1561 tgatatctca atgcctttca cacctatcaa ttttgtcaca cttatctttt cctaggttct
     1621 attctatata atctagaaaa acaaagaagg agacaccatg aaaaaattca ttattccttg
     1681 tatatttctt ttcttgagta ttttgttatt ttcaacttcc atcctagtcg gttcttacct
     1741 cgattcaagc ggaatgctga ttgaaccagc gttcttttgt gttccgttgg gctacttaac
     1801 attaggactt gctttcttta ctgccttata cagtttcgtc aaacaaagat tattgaagaa
     1861 atactcggct tgatttgcac gtaaatcctc tgttgaagaa aagttagttt tttcaacaac
     1921 gaaccttcga gtattaagaa tggatgttga tgtagacatc aaacgtctgc ataaacattc
     1981 attttattcc tccattacca acaattattc ccaatcgcca aaagaagctt ttttcggaat
     2041 taccttctcc attttttcac ctgctggtta tattgtcttt tgtaataccg atagtcctct
     2101 tcaatcattt tcaatatgcg aaaagaacca attttaacat ggcactcaaa tcacaattcc
     2161 tagggcggcc aaaaagatac ggctcgttga tttcaagggt ttcaacgagt tcattgacaa
     2221 acccagcggt gctatcttct tttggaataa gaagttgtga tatctaaagt gagttgattc
     2281 atgttataat ttgtatgcat gattttgatc ctttcaaggg ttgaatgatt tagagtgcga
     2341 gtatcctctc gtactctttt attttaccta aaaagctcca gaaatgacga atgactcgtc
     2401 atttctagag cttttttcag agaattatgt cacaatctct ttttgttata ttgatattct
     2461 atatcttttt ttattggttt tattcccttg ttcgttatag actaaaatcc ttctttcagc
     2521 attattcaaa tcaaatggca acataataga gaaatttata gtatgtataa tcatctgaat
     2581 tcaatcaaat caaccggagt catatacgga gtcaaactat cccactgaga gtttattaaa
     2641 cgcagattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag
     2701 ttccgaccaa agaattataa ggagtgaaga caaatgacct atacccatct tacaacaaat
     2761 gaactcgtga tgatagaagc ctatttcaat atgcatcaat ctgttaccgt agtcgctaag
     2821 acctcaaatc gttccagaca gaccatctac aatgtgtaca acttcctgaa acaaggccaa
     2881 tctatccatg actattacca acaatatcaa aaaaataaaa cacggtgcgg ccgacgaccc
     2941 atttgtttgc cagcagaaca agatgcgtat atccgaaaca aggtggcgca aggatggaca
     3001 cccgatgtga ttatcggtcg tgctgagttt tcaatttcat gttctgttcg aaccctttat
     3061 cgcaagttta aaacaaacta atttgattgc tcgaaactac ccatgaaagg aaaacggaaa
     3121 cccaacgggc ataaagaacg tcgagggaaa caagccttca aacggaatat ttcagaacgt
     3181 ctcacggact atcctgaatt tcaacaggaa tttggacatt tggaaggcga cactattgtg
     3241 ggtgtccatc ataaaagtgc tgtcatcacg ctggttgagc gcttgtcaaa agtgattatt
     3301 actttaaaac ccgaaggacg gaaagcacta gatattgaaa aagtgacaac tgactggcta
     3361 caaaagcttc cccgaaactt gttcaaatcc attacctttg attgcggaaa agaattttcg
     3421 aattggaaaa acatgagtaa tgctaacgat atttctattt attttgcaga tcctggaacg
     3481 ctgtcacaac ggggattaaa tgaacattct aatggattgt tacgtaaaga cggacttcct
     3541 aatacaatgg atttcaatca ggttgaccaa aaatttgtgg cggcggttgc ctccaaaaga
     3601 aatcatatac caaggaaatc tttaaattat cgaacatcgt tagaagtatt tttgagttac
     3661 ataagtgagg agcaattgtc taacttaatt tgacatttag aaaataataa accatatccc
     3721 atctattcaa ataatatttt accgattgaa agacttgcta atcaaagcag aatagtttat
     3781 atagataaat tattttcttt aaacttatac tcatttttag atattttata agaatacttc
     3841 atgtcttcgc cagtaatata aagaacatct gtctctccat ttggacttgg gttatctatt
     3901 ttgggtgtaa catactatac atacaaccga tacataaaag atttgacatc tggacaaaac
     3961 acaatgcttt acttcatttt tataggcttt gtttacacca tcacaaaaat cacctcctaa
     4021 tttacaaatt cattaaacaa acagaaataa aaaatcacca cgatagccgc gttcaaaaaa
     4081 atttacaaaa gagaatttta gttaaagttt ttagattcgc cccactaatt accaaaggta
     4141 ttttatttat tcttaatacg aaataattta ttgacattta gattaccaat ggtatattat
     4201 ttataaataa atggaggtgg attatgtaca caattattct tgcaagccac aaaaatttag
     4261 cgaaaggaat gaaagagaca gtcgaatata tccttggccc tgcaccattc ataaaagtaa
     4321 tgactgcata cacagacgat aactatgata ttgaaaaaga tacgaatatt cttctcgatg
     4381 gaactgataa agtccttgtc atcactgatt tacttggtgg tagcgtaaat aatcattgga
     4441 tgaattatgt ttacgaaaaa aaattaacaa aaaaaattac agtaattgca ggtatgactt
     4501 tgtccttaat tatggaatta agtatgaaca ttgaagatta taaattgaga gaaaaaattt
     4561 ccatgattat tgctgaaagc caaaagagca ttataaactg ttcagaatta atggaggtag
     4621 aagataatga ttaaactatt aagaattgac gatcgcctga tccacggtca agttgcaatg
     4681 gcttggacta agtccgtatc tgctgactac attattgtag ttgatgacgc ctctgctaat
     4741 gacaaaatga aacaaatgat tttatcatta gcaaaacctg tatcaacggg actagatatt
     4801 attacttcag cagatttttt aagtaccttt gaaaaaagga aaaatcaaaa tttgatgata
     4861 gtcactagtt cccctaaaag cgcatttgaa attgtaaacg ctatcccagg ggaagtcgat
     4921 tcaattaata tcggtggttt acggtacgcc gagggaaaag agaaagttaa cgagtatgtg
     4981 gctatcacat cagaagacaa aaactactta gcaaaattaa aggagtccgg cttcaaatta
     5041 attatgcagg ctactccaac tggaaaagcg aagatttatt agaaggtggg gaataagaat
     5101 gattgaatgc attttaattg gcatagttgg gtatttaacg acaatagatg aaagatattt
     5161 tggggcatct atgatgaatc gaccgataat agttggacct gttgttggac ttatcttagg
     5221 ggatctccat caaggcatct tgataggagc agcattagaa gcaatgttta ttggcattgt
     5281 aacgattggt gctgcactac caccagatgt aggtgtagct ggtactattg caacggctct
     5341 agcaataaaa agtggagcag gtgctgatgt agctgttaca ttggcgatgc ctttcgctgt
     5401 attggcacaa ggattaaaca tgttggcttt tacgattaac tcctttaccg tttcacaagg
     5461 tagaaaagca attgaaaacc gcaaattatc atcttttcag aagtggcatt tttttccatt
     5521 aatcgttcgc ttaccttccg cagtacttac atttttagta gtatattttg gtgccaatgc
     5581 tacagaaagt tttgtaaata ttatgccaga aaaattaatg accggtttca ccgtagcttc
     5641 tggtttactc cccgctgttg gatttgccat gctaattcaa ggaatgattg acaaaaaatc
     5701 agttccttat tttttcattg gatacctttt agcggcatat ttaggagtcc ccgtagttgg
     5761 tgtagctgtt gctgcaatta tggttgcttt attagttatc caaactaaac gcaatcctgt
     5821 aaatatttaa agggagagtg ataattatgg gagattctaa attaactaaa aaagaaataa
     5881 acaaggtttt ctggcgttca tttgctttac agggatccga gtcatacact gatatgcaag
     5941 gaatgggata tagtttttct atattacctg ctttaaacaa aatatatcaa gggaatgatg
     6001 aaaaatatta tgaagctgtt taccgaaata cggaattttt taatacaacg cctcatattt
     6061 caacctttat tatgggtatg tcattagcac ttgaagaaga aaatgcaaaa acagatcaat
     6121 ttgaaactga aacaatttca tcgataaaag ctgcattgat ggggcctgta gctggtattg
     6181 gtgatagttt ttactgggga acatttcgcg ttattgctgc tggcatcggc ttatctttag
     6241 ctgcccaagg aaacatttta ggtccaatat tgtatttttc aatttatacg ctgttacatt
     6301 ttcttggcag atatggctgc ttacgtattg gatatcggat gggagcaaac gccattgaaa
     6361 aagcatttga gaacaattac cttgaaagtt tctcgttagc agcatctatc gtaggtttaa
     6421 tgtcaattgg agccatgacc tcatctctgg tacgctttac tattcccgcc gtatttaaat
     6481 caaaaggtgg agaaataaat gttcagcaaa tgctcgatga tatttttcca ggaattctct
     6541 cattggggct aacattcttt gtttactttc tattaaagaa aaaagtaaag gttctttgga
     6601 ttatacttgc tatcttcgtc tttagtattg ttggagttta tattggatta ttaggttaat
     6661 taatccagaa taaaactaga tcagacatca acttaaacca taaaataccc aaagataaat
     6721 gtaaatatga gtaaaaagag catggtataa attattagga tctataattt atgggtctga
     6781 tgaatcagaa ttgattctgt agtgacctaa aaaagataga tgttttcgga gtggagaaaa
     6841 ttcactccgt ttttctttgc ttttcttgat gattttagac agctttgaat gctattactc
     6901 taaattgcac cagactctgc cagtttagtt tccttttcat acgctcatgg ttgtagtaat
     6961 gaatatagcc atcaatggct tgcttcaatt cttcaaagat acgatagtct cttccataat
     7021 aaatttcctg ctttaataga ctaaggaagt tctcaatgat cgaattatct aagcaggtct
     7081 ccttacggga catactttgg aagatcttat gttctttcaa cttatgacta taagctttca
     7141 tttggtaggt ccatcctttt tggagtgaaa agttcgacga tagatacatt ccgttgcgca
     7201 gatagcttcc tctaaagctt ccatgatagc caacgcactt ggcttttcag aaatgcgata
     7261 aaataaaatt tcactgttgt acatatctaa aaatggatca agatacagct tcttttcacg
     7321 attggcaccg tatttatcca tttcaaaata ttttagttct gtcgtatctg ttgtaacctt
     7381 ttgatgacag ttgcttgtgt aaaagcgtcg atgaattcga ttttttgtaa tcatgccgat
     7441 tcttccacga taagtattat agcggcgata ttttctcgta aatgatcgta cttcaatact
     7501 cagctttcga atcaaacgtt gtactttttt cttgttaaca tggaacccac ggtcccacaa
     7561 ttcctttgtc atacgtaaat agccataatc tttatattga cttcttattt taagcatttc
     7621 ctcttcgatt tcttgatctg aatttcttcg atcaaatcgt ctttgccaat acataaaggt
     7681 taattcagga aatttcaagg tttcaagaag ctcaactaat ttgaatgatc ctcggaggtt
     7741 gtcgatgatt cgtgcgattg attcgttcgt ctttgttaga cttcctgttt tctaagcttc
     7801 ctcaattctt ttaaaaaggt attctcaatc tgaaggaaac gcacttattt ttccaattct
     7861 ttgatacgat tatgctcttt aggcgtcagt ttgcttgttt cttcctttta ttctttctta
     7921 gacaaaatgg aggaggaccc tttcattttt gagagcccgt gcaatccttt tgaacaaaaa
     7981 ttttcatcca attgacaact aacgcaggtt ggtttatccc taaatgattc gccacttcac
     8041 gataggatag ctcacccgtt ttttataact ctatcgcatc tatcttgaat tgaacagaat
     8101 aacttcgctt atttcgctta cgcaagaggc cttcttctcc aaattctcga taagcatttg
     8161 tagcgacccc aaaaagttag acttttatgg agtggagaaa tccactcctt ttttgtacga
     8221 taaaattaac tgttagacag ctgtttttac agcttctcga aactgaaccg gacttcgcca
     8281 atttaatttc tttttgatgc gtttatggtt gtaataatag atatattggt caatttttgt
     8341 tttcaattca tctaaactcc gatagacttc tccatgaaaa atctcttgct ttaataaacc
     8401 aaagaagttt tccataggtg agttatctaa acaattccct ttacgagaca tactctggaa
     8461 gattttatgc tgcttcaatt tattcttata agcattcatt tgataggccc atccttgatc
     8521 tgaatggaag gttctacgaa atggacaatc atttgtcatc tggattgctt cttctaatcc
     8581 ttccattatg gctagtgcgt ttggtttttc agaaatccga taagatagaa tttcactatt
     8641 gtagaaatcc atgaaaggat ctaaatacag ctttttctga cgaacgacac ctgtggtgtc
     8701 tacttcataa tacttaaact ctgtggtatc agttgtaatt ttttggtgac agatatttgt
     8761 ataaaaccga cgatgaatac gatttttggc gacagtgcca accttcccat gataagaatt
     8821 gtaccgtcga gattttcgag tgtaagactt tacttcgata cctagttttt ttatcagacg
     8881 ttgaatcttc tttttattga catgaatccc ttgattctct aattcttttt ttagtcgcag
     8941 acagccatag tctttatgct ccttgcgaat ttcaagcatt ttttcttcaa tttcttggtc
     9001 tgggtttggt ctgtcaaacc gtttctgcca atacatatag gttgctttag gaaatttcaa
     9061 tgtttcaaga agctcaacta gtttgaatga tcctcggagg ctggcgatga ttcgtgcgat
     9121 tgattcttgc gtctttgctg ggcttcctgt tttcgcagct tcctcaactc ttttaaaaag
     9181 gcattctcaa tttgaaggga tcggacttgc ttttctaatg ccttgatacg ctcgcgttct
     9241 tcagttgttg tattttcttt tttgttttct ttttttctag tcaaagtaag tggtcgccct
     9301 ttctcttttg agagcccctc aatacctgct tctctgaaat tacgcatcca attggcaata
     9361 agcgcagggt tgttcatttc taaagtgctg gcaacttctc gataggacat ctcacttgtt
     9421 tgatataact ctagcgcatt taacttaaat tgaacagaat atttctgatt ttgatgcttt
     9481 ttcaatagac cttcctctcc gtattcacga taggcattca cccaatcact tacttgagac
     9541 ttgcttgaaa taccgtattt gttggcaaga tgccgatatt cgcccacgcc actaagatat
     9601 tctttaacga tttttagttt taattcaaaa ggatattttg ccatacaaat aacgaccccc
     9661 aaaagttaga ttttttggtc taacttttga ttgatccatc cgtgtacctt tttttgatct
     9721 cactccttgt atttttgcta aatatcctaa tccaccttct ccttcaagat actcttgaac
     9781 gattgttgac ttgaattcaa agctatattt tcccataaaa ataccatccc ccctgaagtt
     9841 agattttggt ctaacttttt agggtttgga catcaccagt cccattttca ttcacgtaat
     9901 gcaagaaact gactgaagtc attaaacaaa aatctgagct agaatgaagt tcattctgac
     9961 tcagatttga ttttgcacaa ctcatcagtc ttcgtaaaat atattttgcc taataagttt
    10021 cttttaaagt atctataaaa cacaaatttt tttgaataac atattccttc cggaatttat
    10081 ttcgttattg tgtgatttct cagagtacaa tggatgtatg gaaaggattc aacgagaatt
    10141 tgctaatcag tcctgtagct ttgtctacct tcataagtct gttatccaaa gcgctgtgtt
    10201 tactatcaac tcattagctc ttttattacc gtattctatt aggcgaggtc gtgaccacac
    10261 agtgagtttc tgtggaatcc cacagtatcg aagcattttc tagaatttat atcgaaaata
    10321 ggtttttatt ccatgaaatt atttgttggt aatgacgtta gttctgaaaa attagacgtt
    10381 tgcttcctaa cagacgacga tcagctatct atattgactg aaaattctgt tgctaatgac
    10441 attgaaaggg ctccattgac acgagaactg attcttgtgt tcaatgaaaa caatcacttc
    10501 ggtcaaatcg ttattgagat ggaattaaac gtccatgtac agctttcatc cttcgatgtt
    10561 tttccatgaa gatgcagagc taaaaaaact caatgcactt gtgaggattg aaaatccttt
    10621 tcggatgaaa caattcagtc gcatgtttga ggaagatacg actgatcgaa acaacgcgtt
    10681 aagaattgct gatttattgc gtattcaacg gttcacaact tctcctatca aagaagagaa
    10741 gtacgtggct ttacagcgtt taacacgcac tcgctatcga ctcattaagc aacttcactc
    10801 gtacaaaaca acactttctg gaaaatctaa cgtacaaatg caatacgctt actcgataaa
    10861 tgcgcgatga atcgacaagt ctctatagtg ccactgtcat tactcttgtg acagaagact
    10921 tcacgctaga tgaattggct gtgcttcctt tggaagcttt ctgtgactta ctaaaagaaa
    10981 aaggaaatgt cgctttaaag atccagaaaa aaatgccaaa tcgattcaac gtgcaattgc
    11041 cgtgagttat cggttagata gtcttgcata agaatccatc aatgtcgttc taaatgtttt
    11101 tgtatgtgaa attcgtgcgc ttgaaaagaa catcaaagac ttggataaag caaccgagca
    11161 atttgttgtc gttattccag aatatcaata tttgactagt gttccaggta ttggaaaggt
    11221 acacgccgct ggattaatcg ctgaaatcgg tcaaattgaa cgatttgaag atcaaacaaa
    11281 attggcaaaa tacgcaggct tatgttggaa aggcaaacaa tctgttaact atcggtcaca
    11341 aaatacacct ctgacaaaac aaaggagtcg ctattttaga tactacttag ttgaagcttc
    11401 caactctgta aaaaaatatc ttcctcaata taaagctttt tatcaaagca aatgtaaaga
    11461 ggtcccaaaa ataaacacaa acgagcacac gtcttaaccg caagaaaatt tacgcgtctg
    11521 gtagatacgc tactacgtaa ccatcaactc tacacgccac caaggaacgt gatagataaa
    11581 taacattctg ttattgacgc aaacccttga tataccccga aaaaatttga ttttggccgg
    11641 ggctagttaa gtacgcttca aaataggtta cacaataaaa atattctcaa tctccaactt
    11701 gtcttattac cattagactt attgacaaag agacaattac catgctcttt tttactattt
    11761 agtctactat atattggttt agacagcctt ccaagtctta tttcatcacg cagcctacca
    11821 agtcctttaa aaataaattg aagctcgcaa taatttcagc aaataatcaa cagcaatatt
    11881 tttgggtcgt acataacatg aagtgcttgt acctactagt ctcgttaagt aacagaagat
    11941 tatctttgct tgactaatca ataaattaag taaataacaa tataatatta tcgagatatt
    12001 tattcatact atcaatgacg ctttaacaac gatatgctcc ctcatgcaat tagccagtgt
    12061 tattttcatc atagcctctt gccttttcac atacaaaaat atagttatga tgagtctact
    12121 atacaaattc cagcaataaa agcccaaagc acaaagctaa gggttaatag gtgtagcatg
    12181 accaaaggag ccatcaatca tcaatgtttc atatgcaagg tttgcacctt tgtgaaaaca
    12241 atcaataaca tgttctatca ttgatttttc acgtttagat aagacaaaat tacttgcaaa
    12301 agctgcaaag tatgcatcac ctgcgcccat tgtatcatga ggtgactcaa ctttgcgaat
    12361 gaatccttca taagtcttat tctctcgagt gtcaacaaaa acagggctct tactaccacg
    12421 cgtaaaaaga agatacggaa cctcaaagtc cttgcattca ctaattaagc gattaatttc
    12481 ctcttgatca tctgtagcga ctgaaaactg tgcaatataa attgacggac aaacatctga
    12541 caagtattca tgcgttctat atttatctaa gtcagaaaaa tcaaaagaaa ctttaacatc
    12601 atattttttt aattctttaa tttttgtttc tgttcgacta tggcaactag tatgaactaa
    12661 tgaactgttc ttcatcaaat caataagttc tgctgagaga gcaggtgtca atcgggagcc
    12721 acgcacgcca cctataaact ccctatctcc atctttaata gttacatgtg cttgctcagt
    12781 aggctcgtca ataacttccg agaaagagat atcaacacct tcagaactga tggcgtgttg
    12841 taataacacg ccatcaggat cagatgatag actaccgaca tagtatgctt taacaccact
    12901 cttttttgca ttcacagcaa aattaactgc atttccccct ggaaattttt gattcaaatt
    12961 ctcatagtaa tcaacaacat tatcgccaaa tccaatcaca tacgctgtca taattaatca
    13021 cctactttta gtattcaact actcggtaat aacgacgttt atccaaagag tggttccgaa
    13081 tatcttcaag atgaacactg acgcgaccta gggctgccca cataactgga gcacaaaagt
    13141 actttcgaaa ttcgtcacta atacctggta gctcataatc cttagtatca aaaacattaa
    13201 atttatctgt atattggacc gcaaattttt caactcgagc atctaaaagc cgtgtttccc
    13261 cctcagtttt caaaagtgtt acacaaactc ctttttcgac taactcgagg gtaccatgga
    13321 aaaactctgg tgatgagaca gactttgtac gcaaccattg agattcctca agcacacaca
    13381 ttgaataaga gtaggtgatt ccccataaat tcccgctacc aatccaaatt tgataaggat
    13441 cttctttaaa ttcttcggca tacttcttcc ctttttcatc agcctgttct ctaactgaat
    13501 tcagagccgt tggtaagttt ttcagttcat cagcaaaacg agtgtattca ggaaattccc
    13561 cattcaaatt catgatagac ataattaaga aataaagtgg catttcttga gggcggccgt
    13621 ctaggtaatt gattgcataa gttgatagag cctctaacgg tgtatcttct ttacttacaa
    13681 taccaacggt tggcacgttt cgttctttta aatattcagc tgccttaata gtttctttcg
    13741 tatctccaga ttttgagtat aagattacta aagatttttc attcaactgt ttatgtccca
    13801 ttaagacaag ctcagctgca gtcatagagt aaacaggaat acttgattca gtcatcaata
    13861 catactcaaa tggttgcatt acggctaccg atccaccaga agcagtaaag aaaatattac
    13921 taattccaga atcataagct tgctttgcaa ttttttctac atcttcacga ataccgtaaa
    13981 tccgtttgcc tttttttttc atttcttttg cattatagtt taacattagt tctctcctta
    14041 caattattta gattattcaa ttaagttgtc aaaaatccga agaaggctgc tatgatacca
    14101 aagataaaga ttccgaataa cacgtaagtt gttttaaagc cttttttaag catccagtaa
    14161 atcagaaata cacataatac tgacagcaga tttggtaaaa taccatcaat gatactttga
    14221 atttttgttg cttcatcacc ttgtccaata gaaccagcta ctttgattga taccatactt
    14281 ccagacatgc ccccaattac cataagaccc aaaattgagg cagcatgagt caacgttttc
    14341 atgacgccag aggattcaat gttctctaat agctttgtac caaagttata tccctgcata
    14401 actccaaagt aacggagaag aaaattaggg acattgaaga ttagtaaaaa taaaaatgga
    14461 cccaaaatat tgccttcttt tgctaatgaa gcacctacac cgataccgat aactaataag
    14521 gtaccccaaa acattgaatc accaatacca gctaatggtc ccatcaatgc agttttaata
    14581 gaagtgattg tagaagtatc aaagtcatca ggatttttgg cattttcttc ttccatagaa
    14641 gccacaattc ccattatgaa tgttgataat ggcggagctg tgttaaaaaa ctctagatgt
    14701 ctttgagctg ctttttgctt ttcttcttca ttgccactat aaattttttc taacgctggc
    14761 atcatagcaa aagcaaagcc catatgttgt tggcgctcaa aattccatga tgcttctaat
    14821 gcacatgacc gaatacaaat ttttgttaaa tcatctttag ttaaagcttt cccactagaa
    14881 gtcatcttca accatctcct cccctcttga acccattgca gctttggttt ccatgtttac
    14941 tataatataa gctagacatg cgccgaagat tgcaatcccc agaatcggca catgtaagta
    15001 gctaaagagt gcaaaaccaa ttacgaaata tacaaagaca tctttcttaa gaagcatttg
    15061 caataaaata gcaaaaccta gtgctggcat aatgcctgca gcaatgttaa tcccatcttg
    15121 gacaaaagtt ggaatagcat ttaagattcg ttccataaca ggacttccta agtaaaatga
    15181 gaatccgaca aataaagccc gtggtaaact ttttactaag ataaaaccta aaacatgcat
    15241 tttcgacaca ttttttagtt cgcccttttc agcatatcta tctgcctttc tagcaatcat
    15301 tggtagaaca agcaatgtta gtagattatc aactaataag aatagcgcag cgattgggaa
    15361 tgccaaggtc aacgcaaatt ctgctccctt gccagttgca atagcaaacg cagtccctaa
    15421 aatcccacca gataccgtat caggcggacg tgccgctccg attgttattg ctccgataaa
    15481 tgctaactcc agtgttgccc ccatcataat ccccatctta agatcgccca taattaaacc
    15541 aacaaagacg gaagatacga ttggtcttga taagttattc gtacctgtca tccagtcaat
    15601 tacgatgaac gcagtcaaga gaccaagcaa aattgcttgt actaagagac tatccatgaa
    15661 attaccccct tattttttta ttacaaagat ttttttaaat ctattgcctt atctgttgga
    15721 acttgttgag cgaacacttc aatgccctct gagaccattc ctttaagttc tgctcgttcc
    15781 tcttctgtta caaaaattgt atgcgtcaaa gcgctactgc cactagtttc cttaacacta
    15841 ccgatattta cagatttaat ttgttcaacc ccatttgcca accgtttagc atcggaaatg
    15901 gttttggtaa ctacaaatag tttgtattta tctgtcttcc cactattaat tgcatcaatc
    15961 gaatcttgaa ttgatttgat aacaaccttc acgttatgtg gtgcggctaa tttcaaagtt
    16021 gtcttttgaa ttggatcatt caaaatttca tcgttcgcta ctaaaatgca atccgcactg
    16081 attgcgttcg tccaagttaa agcaacttga ccgtgtatca aacgatgatc gatcctcact
    16141 aatttaatca ttctcttccc tcctttattt aaaagtcatc aaccgtttgc tcataatcta
    16201 gctgattaca aaacttaatc gaaccagaag atgcttgaat tgcatgatca ataatttctt
    16261 caatctgatc agatgaaact aattggaatt ttaaatataa atccactaaa agacttaaat
    16321 tcattccaga aatcaaatag actttagaaa gatctggcaa ccgtcttagc cactcattat
    16381 taatgcttcc accaaaaatg tcggttacaa caatcgtctc atttcccata gattccttta
    16441 gataatcatc gatgatcatt tctacgtcat tatttccatc gacataggca catcgaaaat
    16501 caatattatc caaatctcct acgataatac tcaaagtttc aatcattcct tttgctagag
    16561 gaccgtggct catacataat atttgtctca tacccgaact cctttcgctt tgttattagc
    16621 aaatactgtg ccaattactg tcaccactta aatgaaaggg cgtacaaaaa ataaagtgac
    16681 actatttcca tcgctgtgaa tagtgtcact ttattttatc aataacttac aatgatgttg
    16741 cacacatatg caatctcttc cataggaatc ttaatattat aaagtttctc aatcttacta
    16801 agtgcctttt gaatagaagc aacctcagtt tcgtatcgct taagaaactg atctattgct
    16861 ggataactac ttactccctg attacgaatt agtcgctcaa tcatacagct gatatgaaca
    16921 taaagattaa ttttggacaa tgatgataac gttttcccca ccctcatttg ataatctttt
    16981 aaactttcct caatttgatt gacaactttt tcggcatcta aaatagtaag actgtcaatc
    17041 aagcggttta atgagctatt tttaacaata ttttgattaa ttgcctcagc aatctctttt
    17101 ccatagactc tcaaaagaac tttatagagt ttgccaattt caccatcaaa taattcttca
    17161 agagaaatat agggaatgtt cgacacacgc ggatcatctg ttccgataat ggcaatgata
    17221 tcctttgttt taatgagaaa ctctaattgc tgagtatctc taagagtttc attttcgtat
    17281 ggcagaaatt caatgcccaa gtctttcgac aaattatttt ccatcatttc tttaattttt
    17341 atggaggtac ccaaaccagt attgcaggaa acaacgattg tacgttttct aattttgatg
    17401 ggatatagaa cttgcttttc aaactgagaa tacggcaatt tttttacaac cttttggata
    17461 tcactcgtac tttgaatcag ttgtgcggtt tcaatagcca tttcggttgt aacattatta
    17521 ataatcattg ttggaacttc gattacttgt tcaatttcag tcttaatttg agctaaactt
    17581 cccatatcaa cgaaaatcac tagtccatct ttaggctttc gatactctat atagtgaagt
    17641 aatctgtcaa ttacttcact aaagctgata tctagaggca tatcaataga atctatgaca
    17701 aactgattaa ccaaacgatt ggcaacatta gcaatactac tagctgtcgc atatccatgg
    17761 gctaaaataa ttgcgttaat gtctttcgct tcatttccgg tctcactaat aacacacaaa
    17821 ctgatataca gtttatccca aatagtcaat gttgtatcca gggcgttctc gaagatcagt
    17881 aagaatacat ttaaatccgt caaatagtta cactttaggt tttcatataa cttttcaatt
    17941 tcattcgctg aagcattctc tttcccatac ccccattgat ccctaatagc gagataggta
    18001 gctatagctt ggaccgtatt gccagaaagg tgaacaaaaa gactgggaga gattttttga
    18061 acaatatttt ggatttccgg taaataatac ttaactaaga cttctgaatt tttttggtaa
    18121 ataaaacgat cattaaattc tgccatttgg tccttcacaa aacgtcgaaa ctcttcacta
    18181 tttctattca catcatgaca ctctgaataa tatttagaaa cacgatgaat caaacctcgt
    18241 aaatttgtag ttacagttga agtctcgtca caatcatgaa catagttacc attactaaag
    18301 atatagaacc gttggctcgc aatttggttt tttaactcaa tattttctgt tagcaagtac
    18361 ctgctaggta aatcagaaat atgaatttcg atatattttt tattgcttcc actaaatagt
    18421 ttattcgcaa tagtttgttt tattagtcca tccagctcac caatgtttcc atacaaatct
    18481 aagcgaatca ggaaactaag ggcggcttca gaaactaata tttctttatt gatcagagta
    18541 gactctttct ctaataaatg gaagacaatc tcttttcgtt cgctaatttc tctatcttgg
    18601 taattaggca aagtacaaac aatcggaatt cttcgtcgaa aagtttctag aaaaaaattt
    18661 tcttcctgtt ccgttgtcgc aaaaatatat ctaacatgtg ataaacgcca aggaccacta
    18721 tctccaactc ttcgaaaaac gcctttatcc atatgaataa aaagtttctc ttgtccttct
    18781 ggtggtaaac gatgaatttc gtcaagaaag acgattccac cgtctgctgc ttctattata
    18841 ccaatatggt cagaatcggc gccggtaaac gatcccttca tatggccaaa caactgactt
    18901 gttaataact ctggattatt aaaatactga gcgcagttta actcgatgaa cggagcattc
    18961 tcatgaataa taccagtttg tcggcaatac tcagctagtt tttccactaa gtaactcttc
    19021 ccaactccgg acgatccaaa atacattatt ggtaatccac ctggatattt cattgctgat
    19081 atcccttgtt gaaccgcttc ttttaaactt tgttttgcac caattaaatc atcaaaaaaa
    19141 tgatctgctt ttttctttga aattttttct ctaaacgaat caattgaaga aaactcaaaa
    19201 ctatctaatg aatgtgatat ataccgttca attgtatgga ctggaaaaaa aagaacagga
    19261 cgaccattga ttttcacaac ttttttctgt tccattagtt cgtttagatg cttactagcc
    19321 gtatttcttt ttatttgaaa tttttgggca atatattgcg cgttacattc ttctggaaaa
    19381 tattgtggaa aatcatagct gttaagtaga tcatttaatt cttttaaaat tttcgttttc
    19441 aaatgaaccc acctccttta cttaataata aatgattttc actataaaat tcaatattct
    19501 ttataaacat ctttccactt taacaatacc tatggtattt ttcctaaaaa atatttgaca
    19561 ctttttatta cctgtggtat tttatttatg caaacaaaat attaccattg gtaatcaaag
    19621 gagagatttt tatgtttaat tttgatcaag aaagattttt aaagactcaa gctggtgccg
    19681 ttgcaacaaa tgaacaaatt tccaatattt tagaatccat cgcatctgac ttagaagaaa
    19741 tttactttat tgggtctgga ggagttggta ttctttttga ttcaacaatc gaattcgcga
    19801 gaactcgtag cactttccct tttcatcgtg ttatcgctgc cgaatttatg attcatccca
    19861 ataaaacatt ttctaacaaa tctttggttg ttatcccatc tttatctggt acaacaaaag
    19921 aaacacttga gttagtcgaa tactgtaatg aaatgaacgt caaaacatta gctttggtag
    19981 gaagtcctaa aacgccccta gaaaaagctg caacatattc tatctataac gacgttttag
    20041 atgatacatc ttcagaaaat tattaccttc aaggactgct gattgctctc ttcttaatga
    20101 aacaaagaaa tgaaatcgat atcaatcaat acaataccta tattgaggat ttaaaaaaaa
    20161 tacctgaagc cttagtgcac gctaaagagc aagcggaacc aatggcaaaa tcttttgcaa
    20221 aaaaacacaa agatacttca tatcatattt taaccggtgc tggtgatagc tttgcggaag
    20281 catattacta tggcatgtgt attttagaag agatgcaatg gattaagact cgtcctgttc
    20341 atgcagcaga cttcttccat ggaactcttg aattaattga agcgggtgta agtgtcatca
    20401 ttacagaagg agaagatgat tctcgacctc tcacagaacg cgtgaagaat ttcgttccta
    20461 agtttacaga tgttcacgta acggttgata caaaggacta tgacttacct ggcatttcgg
    20521 aagacacgcg aaaaattatt tccccagtcg tcttagctac tgtctttgaa cgaattagtg
    20581 cttttctaga agaagaacgt aaccaccctt taactactcg ccgttattat aagcaaatgg
    20641 aatactaatc ataaaaatat gaaaaaggtg accgcgactc cattgcccat ttgattgctt
    20701 cattttctct aatgggagaa tagaatcgca gtcacctttt ttaggaggat ctaaaatgaa
    20761 aatcgcagtt atctgtagtg gtggcgactc tcccggaatg aattcagctt tattagcaat
    20821 aaaaaaaaca attgatagcg aaccagattc ggaagtagtc ttttttaaga atggttatgc
    20881 cggaatattt aaggaatgta ttgcctcagt ttctctaaaa gaagttgagg tattcaaagc
    20941 tggaacactt attggaacag ccagatttga gaaaatgaga gatctcgaaa gtcggaaaat
    21001 tattattgaa aacctaaata agatggagat cgacaactta attgttattg gaggtaacgg
    21061 ttccttgaaa ggagctgaac tattgaaaaa tgaatcagaa ttacaagtat ctgtcatccc
    21121 agggactatt gataacgatt tccccaatac cgattataca cttggcttcg atagcgcctt
    21181 aaacactata atccgatcaa tcgatgattt aacttctact gctaatgctc atgatcgtat
    21241 tattataatg gaagtcatgg gccgaggctg ctttgctctt ccagagtacg cttccattgc
    21301 aaataatact tattgggttg agagagacga caaggaatac ttacacttaa tatgtaaaaa
    21361 atttgagtcg gagttctact cgcctatatt gcttttacca gaaggtatat ctaacaaaca
    21421 aaaaatcatt gaatttatta ccaaaaattt accaaaagaa accaaatact taaatcttgc
    21481 gtatcttcag cgtggatcat ctccaacttt ttatgaccga tatattggct atttgtgggg
    21541 tcaattagcg gcgaaggaat taattgaagg aaaatctggg atattaagtc aaaaaaatgg
    21601 taatataatg ttaatacctt ggtaaaagga tggattacta tgatttttga caaagtaaaa
    21661 gaacaaattc gaaaagtgta tatcaccact gatcggaaat ctgaattatt gccttcggaa
    21721 agagatttgg cccaggaatt ttcggtgagt cgtccaacaa ttcggaaagc tttagcttct
    21781 ttagaagacg aaggaattat ttctaaaaat gaaggtcgcg ggtatctagt gaaaccagct
    21841 tcagaaaaat atgttgacca cgaactcgac tcttttatcg gattcttcca agatgcggtt
    21901 cagcaaggaa agaaaatcag ttctaagatt atccaacaaa ccatcatacc tgctgattca
    21961 acaattagtg aatgtttgga gattgagcag aatactttag tgcttgtctt ggaaagaatt
    22021 cgatacatcg atgactatcc aacttgtatt gcaaaaagtt gcattcccct atctgtttta
    22081 cctgaaattg ttgaacatga ttttgccaaa gaatccctgt tctcagtatt agaaagtagg
    22141 ggaattagat tagcttatgc caaacgctca attgaagtta caccgccaac agatttagaa
    22201 aacctttatc ttgatttaga aaaaaatgag ccagtctttg tctttacaag tattggattt
    22261 gctaaaaatc agaaaccgtt tgaatatgaa atatctaagt accctgctta taagacaaag
    22321 tttgaaagta ctgtttccaa ataattttta aatatgctta aatgtataaa gagcctcgcg
    22381 gataacagat aaagtgtctt tccgcaaggc tctttttcta cttcctcttt atcttaatcc
    22441 gctaagtagc ctacagtcca aacattatca tctaattaag gtgtgtggag gtactcctct
    22501 accctaagtc aaggatagtc gacatagttg ttaataagtc ttcaaagcaa gcctgaacct
    22561 ttttcattag gtgtgcttac ttcacatatg acgctatcaa aaatgatagc acccgttgaa
    22621 tcaaccaatt ttctctaatt catatcccca ttcaaacaac tatattttaa aaactttttt
    22681 aatctttgat tatgtgtagc aaatttgtct aatttttatt tctttgcaaa atttttccaa
    22741 atttaccaga taaaagtaat tcatattcat gattgagtcc tttaccaata aaacctattt
    22801 cgcaaaaatt atttgtaagc attaaggatt ttctgctgac tttcactgga aggataaccc
    22861 aagaattctg accaaagtga aagattaaga accatctttg ccagtctatc tatgtgctat
    22921 tgtaactgtc aacatcatgc gggcagttat tattatttat ttgaataata tttttattat
    22981 aaatcagttt tttagattgt gcataatcac taattgctgt gattaattac agaatatggc
    23041 tctctgggaa aatgtgcgcg tcatgtattc aattttaaat tctaatttgt acaaaatgaa
    23101 tcgtcacgac tcgatctgac gcaactccaa atgggggcgc cgacctgtac caagtacagt
    23161 ggcatgttct ttaaacattt gaagcaaaat ctgtccatca aaaacctgta ttttcaaagt
    23221 gaacaaggtg cgctaaatca ggaaatactt acactgatca tttcttaatt tacctactta
    23281 accaaaattt aaatcaatat gaaagcaaca cttttcaaac taaagaggtc tttttaccgc
    23341 ttgatgtttg cttcgataaa aaagttgatg tcggattagt tttcatttta ataaaacgta
    23401 ctattcctga aaaaaattta tccactacta agtattcgga tttttctaat ttttaacaaa
    23461 atttatttcg aaattttctg atattatatt cgcaacatta gtgaaaattc ttagataaat
    23521 tcattctttc aatctccgaa ttttagatat atgtgaaata tctgtttttt gaatgtttgc
    23581 ataagttaaa agatgagcgt gctttagtat tctttagaat agcttcttat acaggggaac
    23641 gaaaaggaga aatactcgga ctgacatgta gagacataga tttcgataat caaactatct
    23701 gcttcaacaa aacgttagta gaactatccg agggctctct atgtattaat cctacaaaaa
    23761 cagacaaatc aaagggagta atttcaattg ataatggaac cttacgtatt ctaaaaaatg
    23821 gaaacgttct gtaacataaa agaacctttc tagagggatt cggatagata atatagatga
    23881 tatacctgta atcagcacta tcaaagggaa taaaaaaagg gacttccttt acaaaggcta
    23941 ccctaaccat gtcatggata aagtagcacg gaagttccca gatatgaaga taatcaaagt
    24001 ccatggcttc agaaagactc atgctaccct tctctttgaa gcaggtgctc aagtaaaaga
    24061 tgtacaagat aggctaggac attcttcttc taaaacaatc atggacattt atattaaagt
    24121 atcaaaaaat cggcaaaatg aaacagtcga attatttgat tcttacatga acggatgata
    24181 agcgaggtca aaaacggagt cagaggatat tatggggata cctttttacc tcttaatcct
    24241 ttaatatcaa tcctttaagc ttcgactttt gcaatcttcc cttgttcgat atagacccaa
    24301 atcgatctat caataattat caaactgagt catatagaga caaaaatttc aaaaacgacc
    24361 tattaattgg tcatcgaata tcatttaaaa tcaatacgac ggagtcaaac acggagtcaa
    24421 aacaagcccg ctgagagtaa aaaatataca gtcccgtagg taaagtgcca caggcctgta
    24481 tattttttat ttgagattac ctgaaatgat tccatttaat ttttatctca ttttttccta
    24541 ctagttagta tcaaatactc tcctattttc tcaataaact attttgggtt acatggtatc
    24601 tgaaacagct aaaaccttgt cattatagca aaaaatcact ttatctccgt ttcatttcag
    24661 atacatttca cttaagcgga ttccaaaaaa acaaaagaaa aaggaacccc tagttagcct
    24721 atgcttttta ttggttcctc tcaaaacacg gtcatataac aacggggtgg ggtaaatgaa
    24781 tgaaaaaggt ggaggaaaca ttattcaata gctgtcttaa atacttcgtt ataaaatgtg
    24841 aacaaataaa agacatctat ccctattttc tacacttcca cttgactacc atctttgaat
    24901 cgtacaattt tcttaactct tctcgtttac ctcaattcat tctcaagtgg aatcaactca
    24961 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggcttcgct
    25021 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
    25081 ggaattggat cttcacctgg tagatattgc agattagaag catgtaattc tttctcagaa
    25141 acaaaccact cattggaaaa gacatgaatc aaatcatcac tcgcttgtgt aaaatagcga
    25201 cgtttaatga caaagatatc ttcattacta tctaaagtcc ccacatcaaa tgcatttaaa
    25261 atttgtcggt aaacttcttt gactccttct gttttattca atttagccaa agctttttca
    25321 atttcttctc gtgcatctga actattcgca gcataagttc ctagtaattg atcaatataa
    25381 gttgaatcaa tatcatacaa cttagtggta ttttcactat aaaattcgat cgttgaaaaa
    25441 tcttctcctt ctttttctgg ttcttcctct attaatgaac ccttcacggt ctcgtacacg
    25501 ccaatttgtt cttccaaaag ttgaatttga ttgttcaaag ttggagattt caccatgtca
    25561 tcattgtaat cgtcataagt cactaacgat tcataagcac gattcaattc ttgaaatgct
    25621 ttgacgtact cgatacggat ttcaagtggc gtatgttcat caatggattc catggatgaa
    25681 acctctttta acttaccata tgcttgtttg aatctttttt tggattcctg ataacttgga
    25741 taaatcaaac ctgattcttc ttttgctttt gaatacagtt ttgtcgcatc ctcgacattc
    25801 ttctccattg tcgctggctt acgaaacgtc acaatcaatc cttttgtctt atcaggatac
    25861 gtacgattcg tacgtgaaaa agcttggatc aatcctgcat actctagatt acgatccaca
    25921 aatagggttt gaatcgtagg ggcgtcaaaa cctgttaata gacgatccac tacaatcgct
    25981 aaatcgattt gtttcccaaa ttgtttgaat tccgctcttt ttcgtgccaa acgattattg
    26041 atgtctccgt tataacgctc aatgtctgct aatgaccagg aagtatcata atactgatta
    26101 tattcctcaa taattttttg catttcttct tgttgggcac ttgaatctcg gctattttct
    26161 tctaatgaat aggtaatagc gattcgtgga aaatcaggat cctccatcgt tcgtccttca
    26221 cgaatgagct gattgggaaa ctcttttgtt aaccaatcag gctctttcgt catctctttg
    26281 atggcccgat aatacttttt ggccatgtca atggaactcg tggttaagat tgcagatttc
    26341 gttggcctgc catttcgaaa atcaaatttt gtataggcat tatctggacg gaacatttta
    26401 tgaatgactt tttggatatg ttcatctgct tcaaaaacag ctgggtccaa ataactttct
    26461 tttttgacag gttccatttg atcaatcata cgattgatct gttctgatga atactctgca
    26521 tatgtttcta cttcttttaa tttccgatag attttattct ctagtgaagt cggctccacg
    26581 gtgttttcat gctctacttg aaatcctaag acagagccat cttctagcgc attcttaatg
    26641 gtataggtat gcaagacctc tccatactga tcatgggttg tacgtgccaa ttgccctttg
    26701 gcttgtttcc gattctcagg aaaaatcggt gtacctgtaa agccaaacca agtggatttc
    26761 ggaaacattt ttttgatttc ttccatattt tcagcactta acgctcgatg acactcatct
    26821 actacaaaca cgatatgttg gcccattaat ttttggaagc gattggttcc ttttttctct
    26881 tcttgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgattaca
    26941 acatttgcat tggcatctgc caacagggtt tcacttaatt ctttggcact tcccgttcca
    27001 acgatcaagc tattcgcttt ggcgttcccg gaagaaatcc cagtattaaa ttccgaagca
    27061 aattttgtaa actctgtggt ggtttgatta tctaaatctt tgcggtccac taacatgatc
    27121 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
    27181 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
    27241 aaggcttgaa tcgcatgtac ttgataagga tgtaacacca tgagggtttt attgtcttga
    27301 tcttcactga cgattgtata atccgcaatc aaacgatgag catccgggat atttaacact
    27361 tgtttacaaa actcatataa gttttctact ttttcattgt ctttcgttcg ccaactaaat
    27421 aaaaacttag ggtgcaagtc tttcggtaag gcatttgcaa aataccgagt ggtttgttca
    27481 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat accttcttct
    27541 gcatattttt tgatttggtt aaatgcctga taaacaccat cttttgcagt tacttgtttc
    27601 agctcgattt ggacaatcgg taatccattg attaataagg tgacgtcaaa acgtcgatcc
    27661 cgtgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
    27721 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcgaatct
    27781 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
    27841 aatggggttt tcgttcgcag cagtaattcc gttttgatcg tttcaaattc tttatcggtc
    27901 aaaggaaaat cattcagttc tgcttgatta tttgagataa ttttttggcg taaattgacc
    27961 caaaggtctt cttctgattt tagatccgga cgatacgtcc actgattatg cccttctcca
    28021 agtacacgaa tcaactgttc ttcgacttct gcttcctcac ggtgactaat tttcattctt
    28081 cttccctcct agacaaacat cttttgtaag aagctttttt tcgtttcttt tagtaaatct
    28141 aacttacgtt gatgaagagc gatcgtatca tctagctgtt tgaagaaagt accgattttt
    28201 tgttgttcat cctttacagg aaccttaaca ctgatattct caatatcaga actgttataa
    28261 gaaggcaatg cccctacttg ggagtatctt ggcaaattta tagtttcaaa caatatttta
    28321 ccaaaataga tatcccaagt attattaaat aaataagcca tggtattgtt atctattaag
    28381 aaagggcttg tgaccaaacg ttttctatta agcattattg ctgcgccaac tttagcaaat
    28441 ataatcgctg gcacatcttt tattggcttc cattttttac gctcaatttg ctcattagat
    28501 acataatgat ttgcatttct catttcatac tcattaccaa tgttattcat gtcggaaact
    28561 ttaaaaaatg gagtaccttc agaaccacct tgctcggcat caggaaatcc aattcccgat
    28621 tgagttttac ctaattctcc caacttacgc tcttcccaat cttccgtaaa accaggaaac
    28681 cggatttctg gcacttttgc tccgttttca gggaacattt tttgtaagaa tccttttttc
    28741 gtttccttca gcaaatctaa cttacgctga tgaagagcga tcgtgtcgtc aagttgtttg
    28801 aagaaggtgc cgattttttg ttgttcttcc tcactaggaa acattgctac tatattttta
    28861 aactttttta gagacagaac atgttgacct gttccctctg cttgaataga taattgtcgt
    28921 tggattccat tgttaatacg tcttaagaag aagtcggaat cagcagattt taaagtaaat
    28981 acaggataaa aatagcttat aatccctcta tcaataatgt cattacggtt aaacacaaac
    29041 acatcattat cacttcgact acggaacgtg aaatatcctc taggtagtac aaagtaacca
    29101 atattatttt ctgtagttac ttgccgattt gcaaaatagt cttcttgcaa aactatccct
    29161 ttttgttgag atgaagttag cataggaaat tgattttgta cacttgtctt ttcaacataa
    29221 tctacaacga agtcccccaa cttcctctgt tcccaatcat ctgtaaatcc tgggaaacgg
    29281 atttctggtt gtgtatcatt gctcatgttt gaacacctcc agcgcacctt gtaaccacgc
    29341 ttcattttca ggtgttgttt gcagggaaga gatagcttct aaaagttttt tttctagcac
    29401 ttgctgttct tgtcgtatct tcttgatgtc atttccgaca tgaaccatat caattggttc
    29461 ttcttcttcg aaagtatcta catagcgtgg gatatttaag ttatagtcat tttctttgat
    29521 ttcatcaaag gttgccaaat gagcgtattt ctcgacatct tttctttcag cgtaggtttc
    29581 gaggattttt tgaatgtttt cttccgatag tttattttga tttttccctt taacaaattc
    29641 acgactagca tcaatgaata atacatctcg agtttgacga ttcttcttca acacaatcac
    29701 cgtcgttgga atcgatgtcc caaagaacaa gttcgcaggc ataccgatca cagcataaat
    29761 actgccatct tctagtaatt tttgacggat gaccccttct gcagcgccac ggaataaaac
    29821 tccgtgtggt aagacgatgg ccatggtacc tgtttccttc aaatgataga acccatgaag
    29881 aagaaacgca aaatctgctt tggattttgg cgctaatttc ccataacgat taaaccgaga
    29941 atcatctaag aaagtcgtat ctgctgacca attagccgaa taaggtgggt tcattactac
    30001 tgcatcaaat gtgtaaggtt catctgttgg ccagtcctta ttcaacgtat ccccattgcg
    30061 gagattcatt tcttcggcgt cgacaccatg taaaatcaag ttcatttttg ctagattgta
    30121 tgtcgttgta tttagttctt gcccatgata tttaacgtta tctgggtgtg ttaagtagtt
    30181 acgtacgttt aacatcaatg aacctgaacc catcgttgga tcaaaaacac taaagaatcg
    30241 tcgttccttt tgatcgagcg ttacgatttg cgccatcata tcagaaacca tatgaggtgt
    30301 gtaaaattct ccggctttct tccctgcttc tgaagcaaat tgactgatca gaaactcata
    30361 cgcatccccg atcacgtccc catcgtgttc taaaacatct acatcattca atttcttgat
    30421 gacttcggta atcgtcacat ttcgttgttg ctcatctgtt cctagttttt ttgattggag
    30481 atccacatca tcaaataacc cattaaattg attgtaggta gatgccaatt gcacaaaggc
    30541 tttgtttaga tcgttcaatt ggaacgttgc ttgttttgct tgatccgcta agacgttgaa
    30601 aagatactct ggtgcaattg catatcccaa aatatcaacg attgtggcaa tcagatcttc
    30661 ttttgattct tcatctgaaa gtaattcacg atacaacatt gtttgtttgc ttactgtgtc
    30721 atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaatc gatcggataa
    30781 atatttatag aaaatcagtc ccaataaata atttttatat tctgacgcat ccattttact
    30841 acggagatta tctgccgcac taaataattt ttgatttaat tctgcaccca aaaagataca
    30901 ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatacc cccttttata
    30961 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
    31021 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
    31081 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
    31141 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
    31201 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
    31261 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
    31321 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
    31381 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
    31441 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
    31501 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
    31561 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
    31621 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
    31681 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
    31741 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
    31801 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
    31861 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
    31921 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
    31981 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
    32041 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
    32101 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
    32161 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
    32221 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
    32281 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
    32341 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
    32401 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
    32461 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
    32521 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
    32581 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
    32641 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
    32701 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
    32761 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
    32821 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
    32881 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
    32941 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
    33001 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
    33061 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
    33121 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
    33181 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
    33241 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
    33301 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
    33361 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
    33421 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
    33481 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
    33541 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
    33601 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
    33661 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
    33721 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
    33781 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
    33841 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
    33901 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
    33961 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
    34021 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
    34081 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
    34141 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
    34201 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
    34261 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
    34321 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
    34381 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
    34441 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
    34501 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
    34561 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
    34621 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
    34681 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
    34741 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
    34801 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
    34861 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
    34921 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
    34981 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
    35041 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
    35101 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
    35161 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
    35221 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
    35281 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
    35341 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
    35401 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
    35461 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
    35521 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
    35581 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
    35641 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
    35701 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
    35761 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
    35821 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
    35881 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
    35941 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
    36001 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
    36061 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
    36121 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
    36181 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
    36241 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
    36301 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
    36361 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
    36421 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
    36481 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
    36541 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
    36601 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
    36661 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
    36721 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
    36781 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
    36841 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
    36901 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
    36961 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc
    37021 atttctttta tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata
    37081 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
    37141 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
    37201 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
    37261 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
    37321 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
    37381 aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt
    37441 gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc
    37501 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
    37561 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
    37621 atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca
    37681 acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata
    37741 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
    37801 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt
    37861 gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
    37921 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta
    37981 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
    38041 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
    38101 ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga
    38161 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
    38221 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
    38281 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
    38341 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
    38401 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
    38461 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagaattat
    38521 gaggaagagt ttttggctct ctatcgttct cttagcactg aagatgcagt tcgaacaatt
    38581 gactatatga agctattaaa aaggcaagaa aatgaacgca atcagctatt gaaagattga
    38641 aaaataaata acccaccgaa ctttaacggt gggtgtattg agatgaaata agattaagag
    38701 ctatggataa cctgtctagt ttaatacaat tgggtctttt ctcttcctta ctctccgtcc
    38761 atcatctctt gttacacttg taaattgata cagaggaact atcccagctc ctgataatat
    38821 caattctttt attgtttcat ctatccctgc ccctttcgct aaatatatta aaaaactact
    38881 acttgctttc acattgtata gtttcttaat atccttagaa gctcgttcaa gagtgaatcc
    38941 ttgtttaaca aagtagcctc tgttttttat taaagcattt ttcacgctgt tatccccaac
    39001 attacgtagg cgttcacaaa cagtgttaat aagttctttc tctgtcatta gatatgtccc
    39061 ttgccacgat aagccctgta aaacttcatg tgcttcatct aaaatcaatt gtttttcctt
    39121 gctgagttct gatcgggaat tttgaggaaa tactctttta gcctcttttt cgttaaaaca
    39181 tatcttaacc cattcataag aaaaatattg atatgtaaaa tgaatttttt ccagaagtgg
    39241 aaggatatct tcattcaatt tttgaatcac agaagttgca ttaaaaacgt ctaatcgata
    39301 gacattgctt ctcttatgat taaagtttcc taacgctttc tcttccttta acctatttag
    39361 gtattcgagc atcttcaaag gaacatcttc atcatctaat ttttttatca atcctataaa
    39421 agtcataagt gatagtaact tattacattt atctatgtca taagttttac catagtaaga
    39481 actcattttt ttagctaatt gttcccccga caaaatacaa gtcacacttt ccttactatc
    39541 atcaaaaaaa ttaacatcag aaagcaagac atcatagaca cgtcgcattt cctctagata
    39601 attccttatt gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa aaagtacttt
    39661 tgattcctct ttcaattttc gaaaatgtgt aggatacaag tctaggttct ctaatagcca
    39721 attaaatgtt tctacccgac tgtctttctt taaaattagc atcacaactc caataaaatt
    39781 ttcactgata tcacaattac tggaatgaca ataaaaaatg gaatgcccag ttttactggt
    39841 aaaaatacta gcagagggat tatgatcgtt gtgaaacaga catcttaagt ttttacttga
    39901 cttaagtaat ttattcatat caatggttaa caatctttca taaatctcat taaaagacat
    39961 gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc atttcttctt tgtcgtcatt
    40021 ttttatcaac ttaacaaaat cggtttcatt gactagttca tactctctcc ctgaatcact
    40081 ttgacaattt ttagaacatg tcatggtaaa atcaatcgga agctcagtaa tatctaacat
    40141 gttgtcttca ttaattatta tcactgcatc tgacttatat cctccaaaaa acattctatt
    40201 cgaagcattt acattgtcat cacacccggg gatgttcttt ttcaagtatt cgtaaacagc
    40261 aaccatttta ccatagtcag taattgcttc ttttaataga aagacaatct tgtatttttc
    40321 tagattttca gagtaccgaa tacttttttg tatcgcaaac gcagaattat gaactaaatt
    40381 gatagataaa accttttcta cgcttaagta tctactatca tctttactca aatctactac
    40441 cttccccttc tcatctttaa cagtgttatc gacatccaga ataattactc tggtttccac
    40501 aaaactctcc ttgtcattgc ctttattctt tgtcttgctt ggaataaatg attgtgatga
    40561 atttagtatc tctacaaact catctcttgt aacttctcta tagttatcta aacacctatc
    40621 tcttacatca gttttttctt tttttgtaac tggcttctct aataattttt cttgatctac
    40681 ataaattgga atattaatca ttgatgtttc catttgtgtt tcctccttat aaaattctgt
    40741 taaagactaa aacatacaga taataaaaac aaactcttta ttactatatg ttttagtctt
    40801 atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat tttttaaatc
    40861 taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg
    40921 ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac tctagagtga
    40981 ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt
    41041 gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt
    41101 tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct caaaataaaa
    41161 gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat cgacatacac
    41221 ataatacctc gataaaaaag gggtgtcaac atctttgtaa aatttattta caacacccct
    41281 tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc caccaataaa
    41341 ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa agagtaaaaa
    41401 attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg acaaaaaaac
    41461 tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat cccgaattgc
    41521 tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag aatatatttt
    41581 gggaattatt ctttatagag atagcattgt actagatggt ataacatttc cagataaaaa
    41641 cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag atacatggct
    41701 ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca agacttgtaa
    41761 taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag aattattcat
    41821 caatgctatt gtagaaaaat ttgacaataa cattcttttc ctcactagca gtatagaaga
    41881 actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca tgtcttatcc
    41941 cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa cgatttcatc
    42001 aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct agtggtaagt
    42061 actccttgta aactaagagg agatgattta atggcaaaaa tatcaaagta caaaaagaaa
    42121 aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg aactggtgaa
    42181 aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa agattatgac
    42241 gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat gacatttgaa
    42301 gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc aacaatcgca
    42361 atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct taaactatcg
    42421 aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt
    42481 tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc aatgcaatat
    42541 attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta tgagaaaaat
    42601 cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa agattttggt
    42661 aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag aaagagtgaa
    42721 gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca tgtcaataaa
    42781 actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac aagagcttct
    42841 agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg gaaattacag
    42901 caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca acatgttttt
    42961 acaacagtta aaaacactct atacattcca aacactgtta atgattggtt aagatatatt
    43021 ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac acatgctagt
    43081 ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg gcatgaaaat
    43141 agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa gaaaacaggt
    43201 caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc tggtcatttc
    43261 tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc tagctcctat
    43321 aacaggttta cccaacactg ccttccatct cataactgat caatcgattg agttcgactg
    43381 catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg atcatttcag
    43441 ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct tctgagattt
    43501 ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca ttgaatggga
    43561 tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata tgagagatcg
    43621 aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct ccgccgtctt
    43681 ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg atcatttttg
    43741 cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg gtgcctcgtg
    43801 caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca cctaggtttc
    43861 cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt actaagttgt
    43921 acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct tttcttgtaa
    43981 agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc gtacaacctt
    44041 cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg aattgccccg
    44101 tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg acggcttttg
    44161 gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct aatttgttgt
    44221 cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat tctttcaaag
    44281 cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc atattatggt
    44341 acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt tcagcttctg
    44401 gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc cagtcacgag
    44461 cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg tctgaaaggt
    44521 cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc agacggaatt
    44581 caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact tcttctgtca
    44641 agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat ttatattctt
    44701 ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg gaaagctgcg
    44761 gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc acatttgatc
    44821 cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg gtcgggatct
    44881 ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc tgcttcttct
    44941 attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact gatggagcag
    45001 ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac ttgcaattcg
    45061 atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc agtcaagctt
    45121 ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa attgtctaat
    45181 ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa ctaaacggtc
    45241 ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg atacttcaag
    45301 gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc cttccatgtc
    45361 taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa cgactcctgt
    45421 ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt gtaattttgg
    45481 caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac cgagtttttg
    45541 caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg tccctgcttc
    45601 gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat cgatcatttc
    45661 gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat ataaaacacc
    45721 aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac agtctagtgc
    45781 ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga tgaccccgcc
    45841 gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta agacattgga
    45901 tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg catcctccat
    45961 atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg ttcgttttgc
    46021 caactgctgc cacgggataa cattagaatg atgttccatg taagagagca ctatctcgtc
    46081 tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc ttgtcgtcgt
    46141 accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc gtactttttc
    46201 tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac cgcgatgaac
    46261 attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt ttggcttttg
    46321 ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt gaaataagat
    46381 aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc ttcgattact
    46441 tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga accgaggaat
    46501 cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat cagatagtac
    46561 atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac ttcgttttca
    46621 tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat caagacacga
    46681 ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat accattaaag
    46741 gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat cgtatgaggg
    46801 gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct gattgccacg
    46861 attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc agcgatcaca
    46921 tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa aatgtgccca
    46981 cggcgattca tataagaagt aatattttct cctaattgat ctacggctga atatttgatt
    47041 tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt tgctacttgt
    47101 tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc tgccacgatc
    47161 aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa ggactcgagt
    47221 ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt cataaaagct
    47281 acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat ataatattct
    47341 tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat gactcctttt
    47401 tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg gacaagcaca
    47461 ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat ggtttgtgtt
    47521 tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg cggcaatggc
    47581 agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc aggttcgcct
    47641 ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt ttctttcatt
    47701 cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt actgatacca
    47761 gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat gactactcgg
    47821 ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg ttgatagtgg
    47881 gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt agcaacgact
    47941 ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa tgtaggttct
    48001 aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa accttcgttc
    48061 agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc atctaacttt
    48121 ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt gatcgctgca
    48181 cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat agctaaaaat
    48241 aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc gtcaaacaaa
    48301 atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga taaggtagat
    48361 tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat tgttaggttt
    48421 acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat ttctaagaca
    48481 gacatgcatt atacccccaa attctctttc gttctcactc attttagact aattgagaat
    48541 gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt aaacaatttt
    48601 gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca gacttatttc
    48661 tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa cagagccgcc
    48721 ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa tcagtttttt
    48781 gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt aaggagagtt
    48841 gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt aagcgaagtt
    48901 tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta aaagtgatgg
    48961 gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat cgaatgttgc
    49021 tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta gaattgtgat
    49081 gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat cttttaattc
    49141 ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga cagcaccggc
    49201 attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa tatgctggaa
    49261 gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat agtcatcgaa
    49321 tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat gttccaaaat
    49381 ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg ctttatttgc
    49441 tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca gcaaaagcgc
    49501 tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc ctattatctt
    49561 ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac gattagtaaa
    49621 ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc ttcctgccaa
    49681 gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa tccgatcatc
    49741 gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg tatcaagacc
    49801 ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag tacaccaggg
    49861 ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc ggataaaaca
    49921 agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt agtaaatgga
    49981 atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact gatgatcgat
    50041 acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga gtaaaggatc
    50101 aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt catatatagt
    50161 gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc tagatatgtt
    50221 tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt ctttgatgaa
    50281 cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca atgaaccaaa
    50341 ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga tgttatagcg
    50401 tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg tcaaagttgc
    50461 caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg aatcagcttc
    50521 tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt gtctgaagac
    50581 agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga ctttgttgca
    50641 gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat tcaatttctt
    50701 attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca atgcgctagt
    50761 agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga taccgacgcg
    50821 ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt ctaacccgac
    50881 taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc cagctaattc
    50941 cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga aatgctggaa
    51001 gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt ttgtaatatc
    51061 ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca ggagacggac
    51121 taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc ctttttcaac
    51181 gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga atgtcttttt
    51241 gacattcgtt agttggatta atgacatgcc attcacatct ttctcttatg atttttcaaa
    51301 caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac ttccgtgtta
    51361 ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg acttagcttc
    51421 tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt acgctcgctt
    51481 actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc aaacagggct
    51541 ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt catataggat
    51601 tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg attttctgat
    51661 tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc cacgcattca
    51721 tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta acagaagaaa
    51781 ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca acttcgatca
    51841 gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac gtgatgtctc
    51901 caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca ttaaataaga
    51961 tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag ttctccttca
    52021 taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat cagtggtcgc
    52081 ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt aaactcgcct
    52141 acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa caggcgacgg
    52201 attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg tacttctttg
    52261 atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt ggcttttttg
    52321 catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc cttgaagatc
    52381 ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg atcatgttcc
    52441 ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt aaaggaagta
    52501 atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg atcaaaccgc
    52561 ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc atacgataga
    52621 ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag ttttcaccga
    52681 taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt acgttgaatc
    52741 ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc tgataactca
    52801 ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac tgataagatt
    52861 tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag aagatcgttc
    52921 ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa gagattccag
    52981 acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca aagtcatctt
    53041 gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca atcagccaaa
    53101 agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg atcagtgcca
    53161 tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac gcaccgaatc
    53221 gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat ttgagcaaga
    53281 gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag atccatttgg
    53341 aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag acaacttgca
    53401 tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct acatataatg
    53461 agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa tcaatacgat
    53521 tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa aaaccatact
    53581 gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac atttgggaaa
    53641 aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc tactgcttat
    53701 ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct gagattctcc
    53761 tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc aatctctagc
    53821 agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg gaaattcaac
    53881 ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg accgattcct
    53941 tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt atcttggata
    54001 ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc ttctcttaca
    54061 agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat caaagagtat
    54121 ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga ttcatgtttc
    54181 gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg cactttagga
    54241 aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa ggtgtgggaa
    54301 gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg ctgagtttct
    54361 tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg cttagttgga
    54421 agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg ctccaactgt
    54481 ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc tctggaaaaa
    54541 tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc accacttcca
    54601 taacttttta tgataattac tataaaaact atttgattta aatatacaaa aaaccatcta
    54661 taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat gaagacaatc
    54721 agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat tcgttcatct
    54781 tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact ttttgaaaat
    54841 tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa atctgtttaa
    54901 agactatcac catgttgctt taggcaatct aactggcggt atctctatga ttcttatgat
    54961 attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt tgcatgatcc
    55021 aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt gtacttattt
    55081 tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg ctgttatctt
    55141 ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg atctttcaat
    55201 tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca tcactgtaac
    55261 tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat tgatctgcta
    55321 tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata tggctcatcc
    55381 gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa caggttatct
    55441 taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt tatctcaagt
    55501 attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt tttatccaag
    55561 ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta ctactgttca
    55621 ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag ttgcggaatg
    55681 tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt tcttaattaa
    55741 agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat cgattcaata
    55801 ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa acagctgttc
    55861 atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca
    55921 agaggtcgta aaagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    55981 ctcaaatttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga acaccgttta
    56041 ttcgaaacaa gaggtcgtac aagacttttg tcacgacctc ttgttctttt gtcaaaatac
    56101 tggtttagtg ctactatgtt cgagtatgct tttagtattt attgacgaaa ggaaccgatc
    56161 aaatgaaatc attgtcacat ccaataaaat ctgtaaagga ttcagcggtc cgcgaaatct
    56221 tacccggttt actgctttct tttgccatcg ctgccttcag caggcttctt gctatttatc
    56281 tgccacagtt aggtggagcg actattgcta ttttattggg tattttatta ggaaatactt
    56341 tttttaaaca gcctgttcta gcaatcggca caaaatttgc tgaaagcaaa ctattagaat
    56401 tctctgtttt tttactaggt gcaactgtca cagctcaaac gattgctcaa attggtgtaa
    56461 aaggaattgt ttttgtgtta ctgcaaatgt cagtcactat catcggtgct tacttgattg
    56521 gtaaaaaact gcaattcagc gatacttctt ctttgatgat ggctggagga aacgctgttt
    56581 gtggttcttc tgctattgct tcaatcgccc cttccatcca tgcgaaagag gaagaaaaag
    56641 gacagatcat cacactagtc aatctactgg gtactgtgat gatgttgact ctcccttttc
    56701 ttggattaca gttgtttggt ggaaatctca ttgaaaaaag tgcacttttg ggtggtacac
    56761 tccaatctgt tggtcaagta gtcgctgggg caagcttact tgacaattct gttgtcgaat
    56821 tcgccatgct atttaagatt ctacgtatta tgatgctcgt tgttgttgtc tatctgtttg
    56881 aaaaattcat gcacaagaaa caaccagctc tgtttgaaac gccaactgat acgcagaaaa
    56941 aacgaaaatt accgcttcct tggtatgtat tagggtttgt gatcttttgt ttggtcaaca
    57001 gcttgttttc tttacctgta gttattgacc aatcggcaca ttttatcagc agctggtttg
    57061 agatcactgc tttagcggca attggtctgc gtttagactt tgcaaaattc atgaaagaag
    57121 gtacacgctt cttatcttat ggattagctg tcgggcttct ccaaacagtg atggctttac
    57181 tatttatttg gctgatcaaa gtttagaatg aacatgaaaa aacagttgtg attctcgcat
    57241 ggaacaatgt ccgaatcaca actgttttta attttgcaga tatccttcta acaacaacaa
    57301 ttggcgtttg atttcaaccc catctattgc gtttcccata tatcctgtca gttttccatt
    57361 ttgcccgatc actcgatgac aaggaaaaac gattggcaat ggattggttc gatttgcctg
    57421 cccaatcgct cgcacagctt tcggactatc aacagcaacg gctacttctt tatacgttcg
    57481 gatttctcca taaggaatgg tcgacaaagc tttccagaca ttttgctgaa aaggcgtacc
    57541 tctctttata gataaaggaa catcaaattc ttttcttttt cccgcaaaat attcttcaaa
    57601 ctgttcttgt gtgcgcgtta aaattcgctg attcgctgat tcgctttctg aaattggctg
    57661 aaaagataca gcagtcaatc cggattcatc tgcatctaac cataaaaagc cgataggtgt
    57721 gtgtattttc attttgtttg ctcctagtca atagactgga acttcctcct ttttctctat
    57781 ccaaaactac ttgccaacta ataaaaattg ttcttaaaag tttgataaac cgaggaaaca
    57841 tggtaaacta aatacgttag tttacaaaaa gaatacctgt tcttttacgt aaaatcaaat
    57901 tttaaaggtg ttatttaagg tttattaaat tttgaatgta aaccatatat aaacatacga
    57961 ttgaaaggat agaaacgatg aaagtatcaa ttgttgtccc atgttttaat gaagaaaaaa
    58021 ctgttcctct tttttttgat gaggttgaaa aattcaaagg tgcccatgag ttcgaatatt
    58081 tgtttattga cgacggatca aaagacggta cgttagcagc aatcaagaag ctagcctcag
    58141 cttatcctta tgtccgcttt gtctcatttt ccagaaattt tgggaaagaa gcggctctgt
    58201 acgctggatt gcaagcttct acaggagatt tagtgaccgt gatggatgtt gatctgcaag
    58261 atccgcccga actgcttcct gagatgatca accggatcac tacttctgat atcgattgtg
    58321 tcggcacccg gcgttcgact cgtgaaggcg agcctgcgat acgcagtttc tttgcgaaaa
    58381 aattctacca gttgatcaat aaaatcagcg atacagaaat ggtcgatggc gctcgtgatt
    58441 tccgcttgat gactcgtcaa atggtggatg ccgtcctaga attaacagaa tacaaccgat
    58501 tttcaaaagg attatttagc tgggtcggat ttaaaacaga atatatttct tatgaaaacc
    58561 gcgaacgtgt ggctggcgaa acatcttggt ccttctggaa attattcaac tactcgattg
    58621 atggtatcgt gaacttctcg gatgcaccgt taacgattgc ttcatttatc ggcgctttgt
    58681 cttgtgtcgg ttctggtatt gcaattttgt tcattatttt gcgggcattg ttgtttggtg
    58741 atcctacatc aggatggcct tccatggtct cgatcttcct gttcattggt ggaatccaat
    58801 tgttatgtct tggaatcatc gggaaatata ttgggaaaat cttcttagaa acgaaacagc
    58861 gtcctgttta tatcataaga gaaacagaaa aagtgaagcc aaatgaattg aagcctcacc
    58921 aataatatac gtcaaatgaa gtaaacattt ttttaaatga aaaaggactg tgacaaaagt
    58981 cttgtcacag tccttttttc cgaataaatg gtgttccaga agtagcttct tcggaaataa
    59041 gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    59101 ctgaccactt ctatcacaac ctctttcata tcttattact tcttcattgc tgcttttagt
    59161 gctgctgcta atgggctttc ttctggttct tctgcttgtg catatttttt cagcaagcgt
    59221 cgttcttcat gtttggtcat tttcttcttc tgctcttttt tatcaagcaa tttttctgtc
    59281 gttccatcgt atttacaacg gaaataagca ccgtttttac cttcgatgat ttccattttc
    59341 cggtgacatt gcgggcatcg atgattagag acttttggat cttttcttct gcggtagttg
    59401 caatctgaat tggtgcagac atagattttg ccgtcacgtg tgtttttctc gcgcagattg
    59461 gccccacatt ctgggcattt cttttgggta atcgagaaat cttgatattc ttgtttgctt
    59521 gatttgatct cattcaccaa gcgttttgtt tctttttcga ttccattcaa gaaggttcct
    59581 gcttgtttct ttcctgcagc aatagcttca aggtcttttt cccagctttc tgtcaaatct
    59641 ggtgttacta aagagggatt taccaatact aataattgct tacctttagg agatacttgc
    59701 agtccttgag gggtacgttc catcagttca gatttgatta gtttctcgat aatctctgca
    59761 cgcgtagctg gggttcccag actgtatttt tccatttgac caagcaagct tgcttctgac
    59821 aatggtttag gcggtgcaga tagttctttt ttgatggcga aatcgggatt gattctcatt
    59881 cctttttccc atttgactgt ttccgtctca gtttcagttt cgcctttcca tcctgcttgg
    59941 acgacacgat tttgccgaaa aatgaatcgt tctttaccaa attcaaccgt aacttttgtt
    60001 tggctgactt tatggggttg agcaaacagt cctaaaaaac gagagacaat catttgatag
    60061 atttttcgct cttcattact taatttttca taacgtgctc tgttttcagt tggcaaaagt
    60121 gcatggtgat cggtcacttt actgtcttgg aatacttttg tctgttggac gatggctccg
    60181 tttttcaaat actgcttgac ctcaggagca aagtcgctga ctgcttgcaa tcgttctttc
    60241 atcgtacttt tcatgtcatt tgtcagatat ttgctgtctg tacgcggata agtaacgatc
    60301 ttatgggtct catacaaact ttgtacaaga cttaatgttt tctttgcaga atagccatag
    60361 cgttgattgg cttctctttg gatctctgtt aaatcataag gcaatggcgc agattccgtt
    60421 tttaattttt cttggacttc tgtgacagtt cctttgcttg tactgatcaa ttgaacaagt
    60481 ttttgtgctt cttcttgtgt ttttaaagca aattgatttt tttgaagcat tttggcttgt
    60541 tcttttccag cttgcaacgt gatcgtgaaa tacgtttgtg gacggaactg ctcgattttc
    60601 ttttcttggt ctctcactaa tgctagtgtc ggtgtctgca cacggcctgc tgataaatta
    60661 tccgcgtact tcacagttaa ggctctggtc acattcaatc ctaccagcca gtccgctttt
    60721 gcccgggcaa gagcagattc atacaatgca tcatattctt tcgcaggtct tagttgcttg
    60781 aatccagttt tgattgcttt atctgtttga gaagagatcc ataaccgctt cactggttta
    60841 ttgaaatgca catactctaa gatccagcga gcaacaagtt ccccttctct tccggcatct
    60901 gtcgcaatga ctgcttctga gacatctttt cgctgagcca gctgcttgat tgcttttaat
    60961 tgatgtcctg ttttaggaag tggtttgatt cctagttttt tagggatcat tggcaacgtg
    61021 tccatttgcc agctctgcca ttccttattc agatcttccg gcattttcaa tcctaataaa
    61081 tgaccaaggg cccaagtaac gatcaccttg ggtccttcat aataattttt gtgcttttga
    61141 ttagcaccaa gcaccttgct taggtctttc gcaacgcttg gtttttccgc tagaattagt
    61201 tgtttcataa aattcctttc tgctttctac tgtgatgggc tgatgatttt tcaacaacgc
    61261 aagtcccttg tattgctgta aatcatcgtc acactcttca caccagcgct catctccgtc
    61321 attccaaaag gcgctgagaa agttactttt tgcattgata tattcataat tttgatggat
    61381 ttcgttccat tgtttttcgt acgcttgtcg tgcttcttcg aaagactcga atgtttcttc
    61441 ttcttcaata tcttcttccc agtcttcaaa aaaccaccaa ggctcattgt ccccgaacat
    61501 ggtaattact tgatacatac tcgtgctcct tctttcagct tttctagact catcttagtc
    61561 acttttcaag aaagttgcaa gaattttctc ttataaaagg gtcatttaat gaacatctta
    61621 aaattgttac aaatgaatct gattgtttaa ggcaaagtag cgataaagtt attgatcatc
    61681 caaaaatagc tgtcttctgg atcgattgtt ggttcttttg taaagtatcc taaaaaacgt
    61741 aaggaaagat aaccgtggat catactacga agttccctgc ttcgatgaac tttttcttct
    61801 ttcgttaatt gaaaagcctc aaatatctgc aaaatgatct gattcgtttc atggattcca
    61861 tctaataaaa cagcttcatt ggtatgcggt acactgatca gtaactcata cactgcttga
    61921 tttgaaaaag caaaggatcg ataagtttct gcatagatct gcagtgcttc ctgaccactt
    61981 tttcccacaa gagcccgtcg cagctggtca ttaagttcct gcagaagttt cgctgttagc
    62041 gctgttttga catcacgaat atttttgaaa tggttataca gcgagggagc cttgatatcc
    62101 aagcgtttgg ctaaatgctg aaaggttatt tgctgaacat ccatctcttc tgctagttct
    62161 cgaaagcagt cgatgatttt ctcttgagac agatttcttt ttttcatttc catgtttcct
    62221 ctcataaaat aaatccttca ctctttcagt ttaccacgaa aaagaaagag aaaaaagaga
    62281 ctttcattga taaactacat attgtagttt ataataaaga gctgtagatg aaaattttca
    62341 gaaagaagga gtctaattgg ctgcaagaat aaaggaaaaa ttatttagga aaagagaaac
    62401 ttgggaacaa aacttgtttg tcttatggtt tggaacattt atggctggta tcggatttag
    62461 tttagtcatg ccttttatgt cactttacat agatacgtta ggtaattata gtacgtcaca
    62521 attgaacttt tggagcggat tgactttttc atctactttc ttggtgacta cgcttatctc
    62581 tccttggtgg ggaaaattag ccgaccaaaa aggaagaaaa ttgatgctgt tgcgtgcttc
    62641 attaggaatg gcaatcgtca tttctttgat gggttgcgtg actagtgtgt atcaattagt
    62701 cgcattgcgt ctgcttcaag ggattttttc tggttatatc agtaatgcca ctgcccttgt
    62761 cgcaactggg acgccaaaag aaaaaagtgg tcaagttctt ggaaccttag caactggttc
    62821 tgttacagga acattacttg gaccgcttct tggcgggatc tctgcttcag cgtttggcta
    62881 tcgccctacc tttttcatta cagggatcat cttatttttc gtcttcttgt tgagtctgtt
    62941 tttcgtccat gaaaaatttg tcccagtgaa aaaagaaaac atggtttctg ccaaacagat
    63001 tttcaaagac ttgaaatatc cacacgtcgt catcggtatg ttcgtcacaa cgatgatcat
    63061 ccaagcatcc aacaattcca ttagtccaat catcagtctg tacattcgcc aattgctgca
    63121 tggacatggg aatgttacgt tgatcagcgg aatcattgct tcgatacctg ggattgccac
    63181 tttgatagcc gctccaagat ttggtcgctt aggtgacaag attgggagtg aaagaatact
    63241 tgcgattggt ttgggttttg cgattctggt ttatatccca atggcattcg tcacgaatgt
    63301 ttggcaatta gctgccttac gttttttgat tggtatctct gatgcttgtc tgctccctgc
    63361 tgtccaagca ttgatcacaa aatattcgcc tcatcaagct gctggacgga tcttcagtta
    63421 taaccaatct ttccaagcaa ctggtaacgt agtagggcct atgatcggct ccagtgtttc
    63481 cagtgcattt ggttatcgtg gcgtctttct ttcgacttct cttcttgttt tggtcaactt
    63541 cttgcttgta cgacacaata cacaagagat ccatcagcaa gaaactgcag aagaaaaagc
    63601 ttcaggagtc tccatagctt cagggcactc tccttctcat agacaataag cgtaaatgaa
    63661 aaacactgct tgaacagttg tcacagactg tccttgcagt gtttttgttt aggcctgttt
    63721 tttcaagaac tcagataaga ggaataatcc cttttccaag gtttcttttt tacaacagta
    63781 acctagtcgg gcatgcttcg gtaaatcaaa ggcacttcct ggtactaata gtactcctgt
    63841 ttcttctaat aaattgatac aaaatgtctg gtcatcttct tcgatggcta atttaatgaa
    63901 agaagtagag acgtaattgg gagcgacaag ttcaactttc ggttcattgg ctacccattg
    63961 ttttaaaatc acaaggttct ctgtaatgat ctttttgttt cgcgccaata ttttttcttt
    64021 attcttcaat gcatgtacag caagatcatc tgacaggact ccgccgcaaa tcatcgtgta
    64081 gtctcgatac tttcgaaaaa cttctgccaa ctcaggtcca gtagctgtcc agccaatacg
    64141 aatacctgga atggagtagg ttttggatag ggaattggtt gctatacctt tttcatagac
    64201 atctacgatc gacaagaatt cacctttatc cgttaaaggc gcatatactt catctatcaa
    64261 tacatatgca tctactgtac gagcaatttc tgcgattttt tctaaagttt tccgatctag
    64321 caatgtgcct gtaggattgt ttgcactatt caaacagatg atttttgttt caggtttgac
    64381 taaggcttct agctgttcta aatcaaactg ccaatcttct tcttccttca aatgaacaaa
    64441 gtcaacagcg gcacctaaag atttggggat gtcatataat tgctgataag taggaagcat
    64501 cgaaacaaca tggtctcctg cttccactaa cgcgtataaa gctaaaagat tagcaccagt
    64561 cgctccattc gtttgtaaga tgttttccgg atcgacggtt tgataaagag aagcgacttc
    64621 ttgtttgaaa acatctgatc cttcgatcca gccatagtcc aatggttcgt ttttgcgttt
    64681 ttcgaaaaat ttacttacgc tagtcccatc taagccaacg agttcgtcta atgtcaatgc
    64741 ttcgatcgag ctttgtgcaa tatcatatac cgctttcttt tcccatttat ttagccattc
    64801 ttctacaccg aaatttgcga ttttcatgtt tctttccccc ttatatcttg atccagccta
    64861 cagcaagcaa cccgattgaa agcagtgcgg ccacacaaat cagcccaatc attacttttt
    64921 cttttcccgt aatttgatgt ttggtttcac gacaagcgat ccagtagaag caaaaaccag
    64981 gaagataggc aatactacat aaaaggacat aagaaaatcc tgctaaaatg atgcccatca
    65041 attcaaaaac tacagcaatc ccaccgatca ctacttgttt tttgattcct tcttttaaag
    65101 aaaactggac ttgatagatc ccgacaagta gataacaaat caaaattgcc gctgtacata
    65161 aagaataggc aaattgatag gcataatcag taactaagaa agtcaataag aatagattag
    65221 tcaagattgc agtaacaact aacgaataag ttggggcatt ttttttattc gtttttcccc
    65281 aacctttcgg caacagaccg tctcgagcca tcaacatcgt tgtttctgct gggagcatcg
    65341 tccaagacaa ccaagaacca atgatcgaaa taatcaagcc gatattgatc agcgctgctc
    65401 cccaatttcc cacgattcct tttaaaatat ttgccatagc aggttgtgaa atagttgcta
    65461 actcttcttg agataagacg ccgtatggca agatcgaagc aaggatatag ataaacaata
    65521 aacaaagcag accaatgatc gatgcttgct gggcttctga acgtttcttc gcgcggttgg
    65581 ctaaaacact tgcgccttcg atccctacaa agacccacat cataaccatc aaacaacctt
    65641 taatctgcaa ccagatcgtt ccttgctgcc ctgttccatt cactagatta tcagatacgt
    65701 taccccaaaa atcagcagtg aatatattca ctttaaatgc gataaataca aatattaaaa
    65761 ataggaataa tggcactaat ttacagatcg tcacgattgt attgatgacg ctcgctcctt
    65821 caactccatt gtttactaat aaagtcagac cccaaataaa aatacttgca aataaaatag
    65881 aaggaacatt ttgtccacct ttgaatatcg ggaaaaattc tcccatggcg ctcatcagca
    65941 tcgttgcaaa agcaacattt cccagccacg cagacaacca gtaaccccaa ccactgatga
    66001 agccacctaa tttaccaaat gaagcttccg cgtaaccgaa aataccacca tctagatctg
    66061 gacgcttttc tcctaaatga ttcagcgata ataccaaagc taaaatacca atcccaacga
    66121 ttatccatga aaggatcgct ggccctggag ctgcagaact agccagatca cttgtgatcc
    66181 caaacacacc gcttccgatt gacgaactga caaccagagc aaccagtccc catttgctaa
    66241 tacccgtcga tttcttttcc atttccatca ccctcactta taaaattgaa caaatcactt
    66301 gcaaaagatt cccttctatt ttgcaactgc tcttgcttaa caaaaagcaa ctttattcat
    66361 attattggat attatgaact ttttacacaa gtatttcaag tataaaatga ataaaaatta
    66421 attttattgc aaaaaaacag ctatgctatc caaaagaaaa ggtgtttctt ttgttagcca
    66481 tagctgtttt gattcattta atatcgctta aacgtattgt aaaacttttg tttcttgata
    66541 tgcgtggtcg atcaaaccac cacccagaca ttccattcca tcataaaata caactgcttg
    66601 tccaggagtg attgcacgta caggttcgtc gaatacgact tctgcacgat tatcatccaa
    66661 taagcgaact gtcacaggaa cgtcttgctg acgataacgg aatttcgctg tacacttgaa
    66721 ttcttttggc attggttcat tcgtcgtaaa gtggatttca cttgcatcca agcttgtggc
    66781 atacaatgca ggatgatgga acccttgccc cacatataat gtgtttgtcg ctagatcttt
    66841 tcctacaaca aaccagggtt cttgcgaatc tcctccgcca ccgatcccta gtccttgacg
    66901 ttgaccaatc gtataatata tcaagccagc atgttgtcct ttgacttcac catcaagagt
    66961 gaccatattt cctttttttg ctggcagata gttgctcaaa aattgtttga agttcttttc
    67021 accaataaag cagattcccg ttgaatcttt tttcttagca gtcgcaaggc ctgcgcgttc
    67081 agcaattgcc cgtacttctg atttttccat gccgcctaga gggaacatcg tttttgaaag
    67141 ctgttcttgt gataattggc taaggaagta agtttgatct ttattattgt ctactccacg
    67201 aagcatgtgg accacgccgt tttcatcgcg agtgacttgc gcataatgtc cagtagctac
    67261 ataatcggcg ccaagatcca ttgcataatc caagaatgct ttgaatttga tttctttgtt
    67321 gcacatcacg tctgggttag gtgtacgtcc tgcgcggtat tcggctagga aatattcaaa
    67381 tacacgatcc caatattctt tttcaaaatt cacagagtaa taaggaatcc cgatttgagc
    67441 agctactttc gctacatcct tataatcttc cgtggccgtg catacaccgt tttcgtccgt
    67501 gtcatcccaa tttttcatga atattccgat cacgtcgtat ccttgttcct ttaacaaaag
    67561 tgctgttaca gatgagtcga caccgccgct catccctacg acaacgcgtg ttttgctgtt
    67621 gtctgtcatg atatcaccat catttcaatt agattctgaa acatctacga gttgaaggtc
    67681 gcaacttttc agtattttac attatacgtc atatttctga aaatttcatc ccttaattat
    67741 caagtcgtaa aaatactgtt ctactggacc tattcagtac acaaacatga tagtcaacca
    67801 aaaaatcact catcttttga gcattcgata taaaaaattg gcatactcat aaaaagggcc
    67861 agcctttcgg caataaggaa taattttata aattgtgaaa aactattttt caaaattgct
    67921 tcttatcgct taaagctgca cgtctcattt acaacctttt ctttttagat gttatgagtt
    67981 gctcagataa gtgattttgt ttactctaag aaaaaaggga ggcaaaagat acatgaatta
    68041 ctttatcagt gatatgcact ttttccatga acgcttatta ggaaaaaacg actttgctcc
    68101 gcggctcttt gcaaatgtgg atgaaatgaa ccaacagttg atcactagct ggaatgcagt
    68161 aataaaagaa accgaccatg tttatcatct aggcgacctt gctatgcatc cgcaatatga
    68221 aaaagcaaat gcagaaattt tggaactggt cactcaactg caaggaacaa tccacttgat
    68281 caaagggaac catgacagtc gcgctttttt ccgttattta gaaaaaaatg atcctaggct
    68341 tactgacgga agtcccaaat tttattttta tgatgtaggt gccatcgtta aattcgacca
    68401 tcaacaatat tacttgactc attatccgat gttgctcggg cctaacgaca ctatccgcaa
    68461 tcttcatgga catatccatc attacagcgt acctatcgag aacgatgtca atgtaggcgt
    68521 agacgcaccg gaaagagaac tcttaaagga aaaactacca tttggcactc cgttatctga
    68581 gcaacaaatc aaagaaatct atgaagcaaa gaggaagtac gataggtcgt gaaaaaactg
    68641 ccctatatca agtattaaac aaataaagat aattactcat atttttcatt tgagtaggct
    68701 tattgccaca ggaacattag gatcagaaat ggagttgtaa caagacttct gtcacaactc
    68761 cattccatac tattcgtctt tttgctcatg gtgttctttt accagttttt tctgtaattc
    68821 tttatcataa tttcgggcag ctgacgttaa aaataaaacg attccgcata ataaaatccc
    68881 gattccggaa aacaagaaca ttttttctac ccctaaacga tcagctaatg gtcctgcaaa
    68941 taataatcca attggacctg ccaggttcat caaagagttg aagttcccta agacacgacc
    69001 taaaacattc gattcataac tctgctggat cattgccatc agcagtgtat tgaaatatgg
    69061 cgttgcacaa cctgctcctg cattcaagat aagaaagtac aagaaccctt gactatcgtt
    69121 tggaaccaat ccgcttgcac cgatcgtcag gcccataatg atgtaggcta taataatagg
    69181 tttcattcgg tctttccatg ttcctataaa actgatcagt gcaccgccta agagcattcc
    69241 gactgcgtag actacttcca ctgctcccgc ctgtccaacg gaaccaccaa agtaatctag
    69301 cgtcatcaat ggatacagac tgattgctgg catgaacaac agcatcacaa atgcaccatt
    69361 gatcgttatg taccataacc ctttattatc catcaattgc tgcagcccaa attttgcatc
    69421 tttaaaaata gtaagcggaa cttccaatat ttcaggagaa acttttggga tcttcacaaa
    69481 aatcaataag ccgacaccaa aaactgcccc caatacatct aataaaataa ggtaattgac
    69541 aggaacgact gaaaacaaag ccgcacctag agctggcgca atgatgaaat tcgctgattg
    69601 gaccattcct aactgtccat tcgtccttgt tatgtgagaa gatggaacga ttgtcggcat
    69661 aatcgactgg atcgttggca tttggaatgt ttgtgcgacc gaacgcataa acaaagacac
    69721 aaaaactagc caaatcggga aggattctga aatcgttccg actactgcta aaataagcgc
    69781 gaaaatcgct acaataatat ccgtgacaat caaaagagct tttttatccc agcgatccac
    69841 tagcggtcct acaaatgggc ttagtaaaac catgggaatc attcctagta aagtagcaaa
    69901 actcaaaatt gtcgccgaac cggtttctct tgtcaaatac cagatgatcg catattggac
    69961 gaccatacta gtgattcctg acaaaaactg tcccgataaa aagaacaaat aatttctctt
    70021 ccaattcgtt aaaacgaccg actgttcatt ttccataaac atccctcttt tctttttcat
    70081 tttgtatagt gtttgcacaa caatcattat acgggattct tttttcaaaa aatacctttt
    70141 tccctaaata acaatcgttt caatttaaat ttctttactt agaaacaata aatcagcaaa
    70201 atattaaaaa taacaacatg tgttaaattt aaaaataaaa aagacaacta atttgcgtcg
    70261 tccttacaaa gttagtcatc taacaatagg ccgtttcaaa actagttgtc tttttatatc
    70321 cacagatcat tcgacttttt cgaataaccc acggctaatc atcccatcca tcagaaaata
    70381 aaatttatct tggctcattt tctgttttgg atctttgaaa atattgactg ctttcaatcc
    70441 atttgctgta gtgatcgaca ttttcaatgt ttgaatatct tttgatacag tgatttttaa
    70501 tttgaatcct tctttgcttt gcggcgtggc atctaatggt cggatcaatt gataattgcc
    70561 tccgtttgtt tctgtaaatc cattatcgcg caatgtatat ctttttgcat caggatgcaa
    70621 acgatagcta attggtgcgc ccagtaccgt tgctgttgtt tcaaatgcca tgattttcac
    70681 ctcgataaaa ttagtttctc tttattataa aagtttatcc tgatttttac taggtttttc
    70741 agttaagttc cctaaaaaat gaaggcgttc tctcagacat ctgttcatat aagaatgact
    70801 tcactagtta ttttttgaca gcacgaacta attgatcaag gaaatactgg atgtcgtctt
    70861 cggtatttcc caaaccaaag ctgatacgga tggattcttt tagtatcggt gtatcttctc
    70921 catacattgc ttctaatacg tgtgaaggct cgatatttcc tgctgtacaa gctgaaccag
    70981 tagaaatagc gaccccatta agatcaagct tcatcagtag caaatcattt gcttcgccaa
    71041 ttaagcgaag attcaacaca tgagggagtt tattcgttaa atcaccattt acttgataag
    71101 ggatttcgtt tgcttctaat gtatcaagaa tcaattgctg gaatccttta tatttgttgt
    71161 tacgtgcttc acgttcttca ggtgacagca gttcaactgc ttttgccata ccccagatcc
    71221 ctgctaaatt ttctgtacct gcacgccgct tttcttcctg ctccccgcct aaaagcaatg
    71281 cagggatatt tactccgtct cgtttatata agaaaccgac gccttttggc ccattgattt
    71341 tatgagcaga gatacttaat aaatcgattc ctaattcttc tggatggatc gtttggtttc
    71401 cgtacgcttg taccgcatct gtatggaaat aagctggatg atccgctaaa agttcaccga
    71461 tttctttgat tggcatcaaa tttccgattt cattgttccc gtacatcaca gagaccaaga
    71521 tcgtatcttc tcgtaacgct tttttgacct cttcgatcga cagattccct ttttcatcta
    71581 caggcagata tgtaacgtca aacccctttg tctctaaata tttcatggta ttaagaacag
    71641 caggatgttc gatagctgta gtgattagat gtctgccttc tttttgacgc gagaacgctg
    71701 tttctaagat agctgtatta tccccttctg ttcccccact gttaaagatg atctcgtgag
    71761 gttttgcttg cagactgtct gcaatgattt gtcttgctgt ttccaatttt tcatgcgcgt
    71821 ggcgaccaaa tccatgtatg cttgaaggat ttccaaacgt gtcttgcatg attgccgtca
    71881 tcgcttcaat tacttgtgga tgcatggggg tagttgccgc atgatccaga taaatagttt
    71941 tcacttcatt catcccttcc aaattattgc taggcacatt ctagcataaa atagcgaaca
    72001 aaccaaagaa gagctcattt ttgtctagag gacgtttcat gggcatagaa aaaaggagct
    72061 gtgacaaaag tcttgtcgca gctccttgaa gctgctcact tctgtcacaa cttctttccc
    72121 tctttttttt gtttattcga attctttttc ttccagacgg aataacggac tcatcggtgt
    72181 attttcatgg atgcgtttga ccgcttcacc cattaattct gaacatgtaa cgattgttaa
    72241 gttttctgga tgacgatttt cttctgtgaa aacagaatca gtgatacaaa tatctttgat
    72301 tggtgcattg tctaaaatac ttttcgcatg atcggacaat aatccatgtg acgcacaggc
    72361 atatacttct tttgcgccgt tttcttttaa tacatttgct gctcgtgcaa gcgttgctcc
    72421 tgtgttcaag atatcatcta ccatgataca tgtctttcct tgaacatcac cgatcacata
    72481 accaccctca gttgaagcat catctgcatg atctacgatg gctaatgtgc tgttcaaata
    72541 ttctgaaaga ctacgtgcac gctgaacacc gctgtttttt ggcgaaacaa ctacgatgtc
    72601 atctcccgtc agacctaatt gacggtaata atgagcaaac aacggcatcg taaataaatt
    72661 atctacagga atatcaaaga atccttgtac ttgaaccgtg tgcagatcta aagtcagtac
    72721 tcgagttgcc cctgcttctt cgatcaagtt agcgatcaat ttcgcagtga tcggttcatg
    72781 aggtttcgcc gtgcggtctt gcctagcata cccatagtaa ggcaatacga cattgatcgt
    72841 tttcgcactt gctcgcttca ttgcatcgat catgatcagt agttccatat agtaatcatt
    72901 gacaggtgca ttcgttgatt gaacgatata cacatgatcc cctcgaatac tttcttcgat
    72961 attgatggaa atctcgccat cactaaattg cttcacgacg cttttaccta attctgttcc
    73021 gaacactttc gcgatttttt cagctaatgg acggttgcca ttcaaactaa aaatcttcaa
    73081 cgtgtcatcc tgatacttat tactcatgta gtcctcccca aaacttctct attcttcgat
    73141 agtttaacat atttttccaa gattttctgt taatttcagc aaattcttaa ggaagtgaaa
    73201 ctgttccgtt actccaactg gcaggaatga ctttatgata atcgccccag ttgtcgtgta
    73261 ctttgtaata acgttctcct gtttcagttt caaaataagc gtacgctgtc acccaatgat
    73321 tgccatatgt actgccaagt actttcaaaa taccaatcat cacaggtttg ccttcacgta
    73381 tacgttttgt agctcgctgc catgaaccta gaagagttgc tcgtgcacgc tcatgattgc
    73441 cattcttctt taaaaaacta gaaatccctg tactaacctg aaaaggaatc gtaggaagtc
    73501 ctagtggttg gatctggcta cgcaatgcct tcgtcaaaac caacgaatca gccgtatttt
    73561 tcttgcgaat ctcatttgga agcatccacc ccttacgata gtcttgataa taggcaagga
    73621 gaactgctgc cgcgtaagta ccacaaagat agcctgaggg ggtagcccat ttcttatacc
    73681 tcaaaaaacg ttcttctggc aatcccaccc agcaatttgc gagttccttt cctgaccttt
    73741 tttcttcgat caaaacggga atatcttctt ttctgttgat ccatctagat aactcgattg
    73801 gctcttcatt gatcagcttc cattttttac aagaaagacc ctcttgataa gtatattttg
    73861 ctggagaata ttctaccgga tgtcctgatt gatcgataat cacataactt tgatcttcaa
    73921 aaaccagagc taaaaaaagc aatttacttt ttcttgactg cagctgctga gtgctggcta
    73981 attttttaat ttttttatct a
//
LOCUS       DACIWG010000006        72792 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.006, whole genome shotgun sequence.
ACCESSION   DACIWG010000006 DACIWG010000000
VERSION     DACIWG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.006"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(19..729)
                     /locus_tag="IYF54_000385"
     CDS             complement(19..729)
                     /locus_tag="IYF54_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ9830748.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="IYF54_000386"
     CDS             complement(726..2198)
                     /locus_tag="IYF54_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9830749.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="IYF54_000387"
     CDS             2336..2809
                     /locus_tag="IYF54_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9830750.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="IYF54_000388"
     CDS             complement(2962..3513)
                     /locus_tag="IYF54_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9830751.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="IYF54_000389"
     CDS             3618..4148
                     /locus_tag="IYF54_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ9830752.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="IYF54_000390"
     CDS             complement(4631..5074)
                     /locus_tag="IYF54_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9830753.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="IYF54_000391"
     CDS             complement(5090..6250)
                     /locus_tag="IYF54_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9830754.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="IYF54_000392"
     CDS             6757..8457
                     /locus_tag="IYF54_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830755.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="IYF54_000393"
     CDS             complement(8559..9392)
                     /locus_tag="IYF54_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ9830756.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="IYF54_000394"
     CDS             complement(9696..11861)
                     /locus_tag="IYF54_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830757.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="IYF54_000395"
     CDS             12121..14415
                     /locus_tag="IYF54_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ9830758.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="IYF54_000396"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="IYF54_000396"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ9830759.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="IYF54_000397"
     CDS             15066..16073
                     /locus_tag="IYF54_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9830760.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="IYF54_000398"
     CDS             16095..17114
                     /locus_tag="IYF54_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9830761.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IYF54_000399"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="IYF54_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ9830762.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="IYF54_000400"
     CDS             complement(18871..20292)
                     /locus_tag="IYF54_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830763.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IYF54_000401"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="IYF54_000401"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ9830764.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="IYF54_000402"
     CDS             complement(22703..23959)
                     /locus_tag="IYF54_000402"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ9830765.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            24539..25840
                     /locus_tag="IYF54_000403"
     CDS             24539..25840
                     /locus_tag="IYF54_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ9830766.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="IYF54_000404"
     CDS             complement(25909..26508)
                     /locus_tag="IYF54_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9830767.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="IYF54_000405"
     CDS             26762..27643
                     /locus_tag="IYF54_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830768.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="IYF54_000406"
     CDS             27656..29266
                     /locus_tag="IYF54_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ9830769.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="IYF54_000407"
     CDS             complement(29343..30002)
                     /locus_tag="IYF54_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9830770.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="IYF54_000408"
     CDS             complement(30285..30950)
                     /locus_tag="IYF54_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830771.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="IYF54_000409"
     CDS             complement(31097..31702)
                     /gene="dhaL"
                     /locus_tag="IYF54_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ9830772.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31730..32713)
                     /gene="dhaK"
                     /locus_tag="IYF54_000410"
     CDS             complement(31730..32713)
                     /gene="dhaK"
                     /locus_tag="IYF54_000410"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ9830773.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="IYF54_000411"
     CDS             complement(32715..33110)
                     /gene="dhaM"
                     /locus_tag="IYF54_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ9830774.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33264..33584
                     /locus_tag="IYF54_000412"
     CDS             33264..33584
                     /locus_tag="IYF54_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ9830775.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33696..34565)
                     /locus_tag="IYF54_000413"
     CDS             complement(33696..34565)
                     /locus_tag="IYF54_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9830776.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34661..35965)
                     /locus_tag="IYF54_000414"
     CDS             complement(34661..35965)
                     /locus_tag="IYF54_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9830777.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(35962..36471)
                     /locus_tag="IYF54_000415"
     CDS             complement(35962..36471)
                     /locus_tag="IYF54_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830778.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36646..38781)
                     /locus_tag="IYF54_000416"
     CDS             complement(36646..38781)
                     /locus_tag="IYF54_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9830779.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38864..40519)
                     /locus_tag="IYF54_000417"
     CDS             complement(38864..40519)
                     /locus_tag="IYF54_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9830780.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40521..40835)
                     /locus_tag="IYF54_000418"
     CDS             complement(40521..40835)
                     /locus_tag="IYF54_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9830781.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41005..41265
                     /locus_tag="IYF54_000419"
     CDS             41005..41265
                     /locus_tag="IYF54_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ9830782.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41279..41593
                     /locus_tag="IYF54_000420"
     CDS             41279..41593
                     /locus_tag="IYF54_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ9830783.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42323..42877)
                     /locus_tag="IYF54_000421"
     CDS             complement(42323..42877)
                     /locus_tag="IYF54_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ9830784.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43003..44932)
                     /locus_tag="IYF54_000422"
                     /pseudo
     CDS             complement(43003..44932)
                     /locus_tag="IYF54_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(45238..45954)
                     /locus_tag="IYF54_000423"
     CDS             complement(45238..45954)
                     /locus_tag="IYF54_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ9830785.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(45955..47778)
                     /gene="glpO"
                     /locus_tag="IYF54_000424"
     CDS             complement(45955..47778)
                     /gene="glpO"
                     /locus_tag="IYF54_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ9830786.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47792..49288)
                     /gene="glpK"
                     /locus_tag="IYF54_000425"
     CDS             complement(47792..49288)
                     /gene="glpK"
                     /locus_tag="IYF54_000425"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ9830787.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49424..50884)
                     /locus_tag="IYF54_000426"
     CDS             complement(49424..50884)
                     /locus_tag="IYF54_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9830788.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50874..52169)
                     /locus_tag="IYF54_000427"
     CDS             complement(50874..52169)
                     /locus_tag="IYF54_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9830789.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52501..53178)
                     /locus_tag="IYF54_000428"
     CDS             complement(52501..53178)
                     /locus_tag="IYF54_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ9830790.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53208..54299)
                     /locus_tag="IYF54_000429"
     CDS             complement(53208..54299)
                     /locus_tag="IYF54_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ9830791.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54400..54834)
                     /locus_tag="IYF54_000430"
     CDS             complement(54400..54834)
                     /locus_tag="IYF54_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9830792.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55418..56875)
                     /gene="ascB"
                     /locus_tag="IYF54_000431"
     CDS             complement(55418..56875)
                     /gene="ascB"
                     /locus_tag="IYF54_000431"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9830793.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56872..58767)
                     /locus_tag="IYF54_000432"
     CDS             complement(56872..58767)
                     /locus_tag="IYF54_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830794.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58825..60600)
                     /locus_tag="IYF54_000433"
     CDS             complement(58825..60600)
                     /locus_tag="IYF54_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9830795.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60650..62146)
                     /locus_tag="IYF54_000434"
     CDS             complement(60650..62146)
                     /locus_tag="IYF54_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9830796.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLYYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62340..63962)
                     /locus_tag="IYF54_000435"
     CDS             complement(62340..63962)
                     /locus_tag="IYF54_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ9830797.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64290..65603)
                     /locus_tag="IYF54_000436"
     CDS             complement(64290..65603)
                     /locus_tag="IYF54_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ9830798.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(65848..66072)
                     /locus_tag="IYF54_000437"
     CDS             complement(65848..66072)
                     /locus_tag="IYF54_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830799.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66092..66460)
                     /locus_tag="IYF54_000438"
     CDS             complement(66092..66460)
                     /locus_tag="IYF54_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ9830800.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(66656..67366)
                     /locus_tag="IYF54_000439"
     CDS             complement(66656..67366)
                     /locus_tag="IYF54_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830801.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67384..67881)
                     /locus_tag="IYF54_000440"
     CDS             complement(67384..67881)
                     /locus_tag="IYF54_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9830802.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(67918..68370)
                     /locus_tag="IYF54_000441"
     CDS             complement(67918..68370)
                     /locus_tag="IYF54_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9830803.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68505..69110)
                     /locus_tag="IYF54_000442"
     CDS             complement(68505..69110)
                     /locus_tag="IYF54_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ9830804.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69560..70180)
                     /locus_tag="IYF54_000443"
     CDS             complement(69560..70180)
                     /locus_tag="IYF54_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9830805.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70182..71051)
                     /locus_tag="IYF54_000444"
     CDS             complement(70182..71051)
                     /locus_tag="IYF54_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ9830806.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71074..71481)
                     /locus_tag="IYF54_000445"
     CDS             complement(71074..71481)
                     /locus_tag="IYF54_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ9830807.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71500..72789)
                     /locus_tag="IYF54_000446"
     CDS             complement(71500..72789)
                     /locus_tag="IYF54_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9830808.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        21056 a        15326 c        12665 g        23745 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
    31141 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
    31201 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
    31261 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
    31321 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
    31381 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
    31441 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
    31501 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
    31561 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
    31621 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
    31681 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
    31741 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
    31801 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
    31861 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
    31921 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
    31981 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
    32041 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
    32101 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
    32161 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
    32221 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
    32281 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
    32341 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
    32401 aattcttaac gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa
    32461 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
    32521 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
    32581 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
    32641 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
    32701 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
    32761 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
    32821 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
    32881 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
    32941 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
    33001 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
    33061 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
    33121 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
    33181 ataataaatt tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt
    33241 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    33301 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    33361 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    33421 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    33481 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    33541 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    33601 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    33661 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    33721 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    33781 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    33841 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    33901 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    33961 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    34021 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    34081 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    34141 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    34201 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    34261 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    34321 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    34381 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt
    34441 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    34501 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    34561 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    34621 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    34681 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    34741 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    34801 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    34861 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    34921 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    34981 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    35041 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    35101 cttttgccca acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    35161 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    35221 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    35281 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    35341 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    35401 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    35461 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    35521 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    35581 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    35641 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    35701 aattcaattt cgtcatcaaa tactcgattg tatattcgat cacaatttct tcattatggg
    35761 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    35821 gcttatgttt gtataaaaac acataacacc agacaccaat aaacgataca aatccgccta
    35881 aaatcggata agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat
    35941 tacctgtatg ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc
    36001 aatgaatgtc gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct
    36061 ctcttctttt gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt
    36121 caatttttca tcttcatatt caaaagattt atttactttt ttgtatagat ttcgattgtt
    36181 ccacctagtc aatacaaatg aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc
    36241 gattaagaaa tacagcattt tcttttctga taaaaaagcg tacaaggaaa aatccttggc
    36301 tctttctggt ctcgttaatg ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca
    36361 tcctaaaaga gccactaacg tttgccaaat ctttaaccag taattacctt ttgaaaaata
    36421 cttatctttc accaaaattg atgcaacttc ttctcttgtt acttttttca tatcctcacc
    36481 tcacgttttt ttgttacgac gtaggagttt accatacatt tcatttcttt tcacataaag
    36541 tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata
    36601 ttttgttctt tttatcagtt gttcttttta ttttttatct cttaattatg ttgctctttc
    36661 caatacgaca atgcttttca ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct
    36721 ttgctcatct ccatcgtttt caaatggata taagattaca tgatcaaatt ccagtccttt
    36781 tgctagactc aaaggaatga tttggcagtt ttctaatcca tatgaatttt ttttaatcga
    36841 ttcttgtagc gtttgggttt cctcttctgt ttgggtgata actgccgctt tttctccttt
    36901 tagagaaacc aaaatctcag ctagtatttt caacaattct tcagaggatc gaaaagagcg
    36961 gtattcgggt tcttttcctt gcggtcggat cgatacgatg tcgatcttct cttgatccac
    37021 agcataggtt ttgaaaagct ctgtgattgc tccgctagaa cggtaactat ttctaagatc
    37081 ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt
    37141 ggaagaatta aagattgcct gattctcgtc acctactaaa gtaaagtgag tttttggaaa
    37201 taattcgatc aatagaccta attgtgccag ggtataatct tgtacttcat caatcaatag
    37261 atagcgaaat gctggtacat ttaccttttc aacaagtaaa tgacgaatag ctaatataat
    37321 gactgcttca tcaacagtac gtgtgggttg ccaagcataa gctgagccat atcgtttttc
    37381 ataaattctt tcaaacaact gatgctcatc cacccatgcc atttcttcga tttgtctggt
    37441 tatttttcga tatttttttt gtaaaagttt ccttgcataa gctacaatac tttgttccga
    37501 atcatcagaa atcaatttac cgaataattc caactgccta tcttctgtca acgagagtac
    37561 ctgattctgc acagcaggac tttttgcttg tttcaaaaga tgattcttcc attcgctcat
    37621 cagtgccttt ttcgttgctt gaagcctatc gatcaattta ggatgagcag gaactttctc
    37681 atatattttt tcgatcgttt ttttagaaag gactgttttt ccatttttag taagtccacg
    37741 gataaaattc ggatgatcta aaaacaaagg atctgattgt tttagattat cgataaactt
    37801 tttgctacgc aaacgttctg tctgctccga tacattttct cccgtgatac gtttgaaata
    37861 agcctcttca ccttctattt cctctgctga aagctgtgaa accagctgta tgatggttag
    37921 attcagtgga tttctttcac ctaatgatgg taggacattc gaaatatagt cgataaaccg
    37981 atgattaggt gatagaatca gcaagtcatc ggatgtcatc gtatctcgat ataaatagag
    38041 cagatacgca atacgctgca tgatagcaga ggttttacca cttcctgcta cgccatttac
    38101 caagatattt tgacttttca catcccgaat gattgtgttt tgttccgatt ggatcgtagc
    38161 agtgatagcc ttcatttcat tggtctcatc ttggcctaag acttctaata atacgtcatc
    38221 ttgaatagct acagacgtat caaaaaactt gaccaaacga tctctttcca aaagaaattg
    38281 tcgtctttct ttaataaccg tatcgatttt attttgacga accgtataag aagatggacc
    38341 aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc caatcataaa caagattttc
    38401 atctgcatca tctgtgaaat ttgctgtacc gatataaaag gattcttttg gctcattatc
    38461 cagaaagtct acaacgactt ttccaaagta gggacttttc aataaacgtt cgatctttgc
    38521 tagctgattt tctgcgtttt tctgtttgat attcatctga tcgatttctc tatttttcat
    38581 ttcaatcatc gaaaaagtat ccaggttatc taagtaactg tcaaaattca atcgaagctc
    38641 gccagccatt tctttcaacg actggtttcc ttctattttt gccgtatcca tcgattcccg
    38701 cagttcgttt tttttatcaa ttagcttttg atacacgttc ttcagatatg cttcttctaa
    38761 ctgtttttgt gtgttgacca tacacattct cctctttttt taaagtggtg gaaccgatta
    38821 gtttacctct tttaagagtc gaatgcaagc tttttaacga gtatcatttg ttaggatata
    38881 tcagttcttc aggtcggaca gtttgatata aagaaaatgc accatcaagg ttttttacgt
    38941 ggtagccaga ctgcttcaag attctttccg ctagatagct acgtaatccg ctatgacagc
    39001 tgacgatata ttcttgttgg ctgtctaatt catttaatcg gtcccgaagt tcattcaaag
    39061 ggatatgttt ggcttccttg aatttgccct tttccaattc ttctgctgtt cgcacatcta
    39121 ataaaatttt tccttttgct agttctgtcg gcaattcatg ccattgtacg gtttcactaa
    39181 gcccttctgc aatgttcatt gctgcatatc ctagcatgtt tactggatct ttggctgagc
    39241 caaatggcgg tgcatacgtc aattctaatt ccggtaaatc gaagatcgtc aaatgccctt
    39301 tgattgctgt tgctaaaata tcgatccgtt tatccacacc ttttgcccca acaccttgtg
    39361 caccataaat ctcgccagtc tctggatgga agatcaactt cagtaaaata tctgttgctt
    39421 ctggataata gcttgcatga tcttttcctg atacatgaat gacttcaaaa gacagttcgt
    39481 tttctttggc tgttctttca cttaaaccgg tggatgcggc agtcattcca aacgttcgta
    39541 taattgctgt tccaataccg ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa
    39601 cttgtctacc ttgacgattg gctggtgaag caagagaaat aagtgcatct tttccagtga
    39661 tttgctgttt tacaacgata gcatccccca ccgcataaat atcaggaata ttcgtttgat
    39721 aacgttcgtc tactaggata cccccgcgta tgcctaattc gattccagcg gtcactgcta
    39781 gctggttatc aggacgaaca ccaatggata aaataactaa atcagcaggg atggtacgcc
    39841 catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa
    39901 tcacttctac tccttgatga gacaattctt tttctataaa agctgccatt tcttcatcca
    39961 gtgttggaag aatatgtggt gcttgttcga tcaccatgac ttctaaacct cttcttttta
    40021 ggttttctgc catttccaat ccgatgaatc ctgcaccaac gatcacggca cgctttggct
    40081 gtttttctaa tgcatgcatc actttatcga tatctggtac atttctaata gaaaaaacat
    40141 tgtcggcttc tgttatccct ggaatcgatg gaacaactgg ttttgcacca ggagataaaa
    40201 ccaatgcatc gtactgttca atgtgttttt gaccctcatg tacgacttct accgttttgt
    40261 tttcgggaaa gatggctgtc acttcatgat ttggacgcac atctaaacaa aaacgagcgg
    40321 ataatgattc tggcgtttgg acaagaagat tttcccgttc actgatttct cccgaaagat
    40381 aataaggcaa gccgcaattt gcaaaagaaa catacggtcc tttttcaaaa acaacgattt
    40441 ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga cattccacct gctacacctc
    40501 cgacaataac gattttcatc ttatttccct cctactgttc gatcaaacca ttgattcatg
    40561 ccaccacgta cattcactac atcataacca gatttcttta attctttagc tgcttgttta
    40621 ctcctcatcc ctgattgaca aatcacatag acggtctttt ctttgtctcc atcatatctg
    40681 tttatacttt gtaatggaac atttttagct ccttttatat gcccgccccg gtattctgca
    40741 ggtgttctaa catcaagtaa cgctggattt tccaaaagct tttcctttaa ttctgggact
    40801 gttatcgatt ttattgattg aaatagcaaa ttcattttct gcacctcatt tacattttta
    40861 taaatacctc acggggtata ttaaacagga gctttattaa tatgtcaacc agaatgactc
    40921 ttcatttttt tcactacttt ttatatttgc ataaaatacc gataaggggt attatctaat
    40981 tataaaaata gaggagaatg tgttatggag attgataata aaaatatcct gaaccgtttg
    41041 aaacgtacag agggacaaat tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt
    41101 atggatgtca tcactcaatt aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg
    41161 atcgttgcag ataatctgaa aaattgcttt gagaaacctg atagtaatcc agaagaacaa
    41221 gcggctaaac tagaacaagc gatcaagatg attattaaaa agtaaaggaa tgatttcaat
    41281 gaaagtcgtt ttccatattg atgaattaga aaaatggtca gaaacaggga aaaacgtaaa
    41341 aaacctgata aaagcttctc cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat
    41401 taccggttat ctagactctg ccaatgcaga attcttagat ttgcaaggag tcgtttttca
    41461 cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa agttctttac ctgaacaagt
    41521 aatcgttgta ccagcaggtg tccttgattt agttgaactg caatcccaag gttatgctta
    41581 tatcaagcct tagtcagtag aggttgcgac agaagtgatc agcttcaaga aatttcggct
    41641 tatttttcga agaagctact tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc
    41701 tgcatcaagt aataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    41761 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    41821 ggtttgacct aagtattaga gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt
    41881 ttgagctaat accgaaggtc agctgattga acaccgttta aggaagagat tgtgaaaaag
    41941 tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gatttctaca tttttgcata
    42001 aatttggctt aataccgaga aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa
    42061 tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagctgctc atattttgcc
    42121 gttttttgag ctaataccga aggtcagctg attgaacacc gtttaccaag aggttgtgag
    42181 cctaccattt aactccgaat cacaagaaat aatttcaaaa acaacttctc ctgtttttgg
    42241 ttcggttggc ttattatcga gaagtagcta ctgggagtct atttatcaaa aaaaggaatg
    42301 cgacataggt cacacccctt ttttattttc tttgaaacat ctttcggtac tcgacagcat
    42361 acaagacaaa agattgtaaa agaccgtaag tgatgacggc aaaaatgact ctcacccaca
    42421 gcgttggtaa ttctgggaaa agcagcaaaa ggatcatgcc aacatagatg acagcagtcg
    42481 ctactttgcc ataccacttc gctccactta atttgatatt tttagatagc ccgattaatc
    42541 cagctacaaa cataaagcct tcttttatca agaatagacc aaacaacagc caaagccacg
    42601 gtcgatacca tgccatagac aaaatcagaa aaagttgtgt caatttatct gcaaacggat
    42661 caagcacctt ccctaagtct gttacctgat tatatctccg agcaatgacc ccgtctagat
    42721 aatcagacaa acctgagaca acaatgaccg ctcctgctat ataaaatgac tgtctcagca
    42781 caaaataaag ataaatcggc agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg
    42841 tttgtatttc tttacgccag tctgtgctta atttcatttt atcgccctac ctcctcagca
    42901 ctcttttctt tttattttag cttacacggg gaataaaacc aatgatttca ctgtttttat
    42961 ttttctttac agacactgat attatgagct aatttgagat aatcacttgc tgttttcatc
    43021 gtttactctt ctttcttcct gtcgtttgcg catttctttt actagtactt tatgagcttt
    43081 ttttgatcct cgctctaggt cacgctgccg tttacgtgtt ttataatcga taaacagact
    43141 gtctaaatcg taacctgtcg ggtacagatc ttctgctgaa cgcaccagac gaacacgctg
    43201 acgaggcacg tggattattt ttttgtctaa atatacatca atcgtatctt cacctatatc
    43261 ttcataaact aacgcttctt tttggtattc attgacaaat atccgatcac cttttgcaaa
    43321 gagctgacgt tcttttttat tctccttcct atttgtaact gctgtcttag gaagaaattc
    43381 ttttttctgt gtccgatagg ctttttccgc caagtaacgc tctgcttgat ggatcaattt
    43441 catcgacata tccattttat gagcaatcca caaagcttgg ctttctcctg tttcttccaa
    43501 cccgcaactg atatttagga cgaagtgctt cccgatcgaa agccatagca gccggcacaa
    43561 aatcttcatg atctccggcg aatttcttga tttctccata atgggtcgtg gcaatgatca
    43621 atgccccttg ctcatacata gattccatga ttgcgatcgc tagtgcagcc ccttcatttg
    43681 gctctgttcc actgccaatc tcatctaaaa gtaccaacgt attccttttt atctttttca
    43741 aaatagcagc gatgttttgc atatgaccag aaaatgtact caacgcattt tctaaatttt
    43801 ggtggtcacc gacatcgacg aaaatctcat caaagacagc aagctctgtc ccttcttttg
    43861 ccggaacttg cataccgaac atcgccatca aagtcaataa accgactgtc ttcaacacga
    43921 ctgttttgcc tccggcattt gctccggtga taatcaagcc tcgatagtct ttaccgattt
    43981 caacatccaa tggtaccgca tgctcaggta aaaatggatg tctgccttgc ttgataatga
    44041 tatgttccga tttattgatt ctaggtgtgc ttccattgat ttctcggcta aattttgccc
    44101 gagcaaagat gatatctaac accgtgatcg tttcgatcaa taaatcgatc acttcttcgt
    44161 tttctgcgat agcaccagtc aatgccgcca ggacttgata ttcttcactg atttcttcag
    44221 cttttaacaa ctgatagtgt tcattcaatt tagaaatcac agttggttca ataaaaaccg
    44281 tcgttctttt atttgattca tcgataatcg ttccatctat tttattttta tagctggctt
    44341 tgataggaat cgtatagtat tcgccttttt gaacaatcat tgcttcttgg atcatttctt
    44401 tgtttttcgg atgacgcaag aatttcagta attttgactg gatttctttt tcgattagtt
    44461 gtaattgttt gcgtacttta cgcaaattac gtgacgcgtc gtcactgact ttattatttt
    44521 ttattttctg gtcaatcaac tcttctacat ttatcaaatc aggaagatgt ttgctataag
    44581 caaacaacaa gggtgtctgg tattgattct tatcaaaaaa ctttgtgatc atccgactgc
    44641 ttctaagaaa atccgcatat tcgatcaaat cggttggctg cagaatcaac ccttttttga
    44701 cttgttctgt cagtgcatcg atcctcggca accccataaa tggaacatgc tgactgcttt
    44761 ctaagatcaa gcgtgcttct cttgtttctt cttgacgatc caagacagtt tgtaggtttg
    44821 tcgagaccgt catatcgctg attcgttttt tgctatagtt tccaattgct cttgcttgga
    44881 tttccttttt gatccgatca aattgtaatt gactaatcat ttcgttgtcc atattctctc
    44941 cttttcttga agagatacct gtcccaaaat aaataagggc ctagagataa ataaaaggat
    45001 agaacacagc ttaaaaaagt catgtcctat ccttattaga atacaaagat attgttttcc
    45061 tgacaggtac cgtttcataa gcacaccaaa taaactttgt ttattggcta ttcgacttac
    45121 tgattagata ccagctacac tcacaaaaca taccccattc ttctaggtct tatttatttt
    45181 ccacttaact atagcaagac acattttggt tgtcaaactt tagtatgttt tatagattta
    45241 caccattaat gaatagacac ctaccgcgat aattgctccg atcattggac ctgcgattgg
    45301 tacccatgaa tatgcccaat ctgaagttcc tttattagct attggcaata ccgcatgtgc
    45361 taatcgagga ccaaagtccc gagcagggtt gattgcatat cctgttggcc ctcctaatga
    45421 aagaccgatg gctaagatca atccacctac agcgaatacg ttcgttccag gtgcaaattc
    45481 gttttgtcca aaagctagca acccaagaac taatacaaat gtaccaatta attctgtgat
    45541 cacatttgct ggataattcc gtatagcagg acctgttgca aacgttccta aaatcgcgct
    45601 ttcatctttc gttgcattcc aatgtggcaa ataagaaagc caaacaagaa tggcccctgc
    45661 aaaagctcct aaaacttgcg caatgatgaa aggaacgaca agattccaac taattgcccc
    45721 tgtcatcgcc atagctagag agactgcagg attcaaatga gctgggccca tgaatccaga
    45781 aatatagaca gccattgtta cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga
    45841 agcctgtgct ttacttttgt ttaaattcac agccgcacac acaccatctc caagcaaaac
    45901 taggatcagt gtcccaataa actcccccat gatttgagtc atatcactgt tcatttattt
    45961 ctcccccttt agttcccgca gatctgattc tgcaatcaat gcttctaaac gtttagtctg
    46021 ttgcgccttc tcctcttctg tccattcaaa atatccagca attgcatcga tcacaggttg
    46081 cttgatctca tcaagctgat ctcgttcaaa caacagatga tttgtacgac gaatgagata
    46141 atcgcctggg gccaaaacca tttcttcttc taaaccataa cgtaaccgag ctgactcagc
    46201 gagactcaaa ccaggataag gtgccatttc ttttgctaat tcaaagatac gtcgggcatt
    46261 agtcccgtaa aaatcagcga tataagtagc atcttcttcc tctaaaccgg ctgctactcc
    46321 ttcttttgct aattctgtca ctgtttcttc taatttagtt ggatcgaagt ttccacctga
    46381 aatctgatat tttttagagt cgatttcttt cgtctctatt ccgtattctt cttttaataa
    46441 ttggcgtatc aatcgcaaag ctccttctgc catcttacgg taatcggtga ttttcccacc
    46501 agataaagtc agcaaaccat ctggttctct ttctaaagaa ctacctctgg aaatcgtaga
    46561 aggtgctttt tcatcacggc tgttttccaa atggttcaac acgtcttcta cttcagcacg
    46621 agaaactttg ttttccttat attcacttac tgtatcgaca actttattga agctcttgtc
    46681 tgaaatcgat ccattatctc caccattgta atcagaacca gaattaccaa tcaacagtgg
    46741 acgaagtcct gcccagcttg cttcgatatc attcaatgtg atatttgctt ctggatagcg
    46801 atgattgatc acatccaata gataatctac atcttcttgt gtgactttcg gatccgtaaa
    46861 atctccttgg taatccgtat ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc
    46921 aaaaaccatc cgcccatctt gctttcctgt atcgaagtat gtgggttgcg gtacaggcag
    46981 ttttttcgca tctacaacta aatggatccc tttagttgga cgcattttag gagagactgg
    47041 acgcgtaaaa ttcaagtttc gtactttatc tacccaagga ccactcgtat tgatcactaa
    47101 ttttgctttg atttcgatca cttcatctgt cagcaggtca cgagctttga cgccaacgat
    47161 ttgatcgcca tcatataaaa agccaaccgc tttcatttta cttactagat aagccccatc
    47221 ttcggcagcc tttttgatat tatcaatcac taaacgggca tcattgttgc ggaaatctag
    47281 atagacaccc gcgcctttta gcccttcttt tttcaaaaac ggttctcttt ccaatacttc
    47341 ttctggtgtc agcgtataat tctcatattt ggttcctgtc acatttgcca gtttgtcata
    47401 aaggtccatt gctactttga ccgagaacat attgaaggtt gttgctcctt cgccctcata
    47461 gattggcaaa agcattggat ctggttttgg gatgtgtggg gcaatacttt gtacgactgc
    47521 acgttcacca actgtgtcag ctactacttc cacatcaaat gtcttcagat aacgaatacc
    47581 gccatgaaca agtttggtcg agcgagagga agtcccttct gcaaaatcct gcatttcgat
    47641 caatcctgtt ttgattcctg atgctgctga ctggactgcc acaccggcac cagtgattcc
    47701 gccaccgatg atcaacagat ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct
    47761 tgtcttgttt gaaaacatat ggatttcctc cttattcaaa tgcttgggta gcttttactg
    47821 cttttttcca accgttgtaa agtttttctc ggcgctcttc ctccatttga acttcaaata
    47881 gcttgccagc ttcataaaat tcgcggatct cgtctgtatc tttccaataa ccgacagcta
    47941 atcctgctaa aaatgctgcc cctagcgccg ttgtttccaa gttttctgct cgttggatcg
    48001 gtacattcaa gatatctgtt tggaattgca tcagatattc attgtttgct gcaccgccat
    48061 ctactttcaa tacaggaatc ttgattccag tgtcatcttg catcgtatcg atgatatctc
    48121 tcacttgata agcaatcgac tgtaaagtag ctttaatgat atcttctttt gtcgtaccac
    48181 gtgtcaagcc aaacatagcc cctcgtgctg cctgatccca ataaggagca cctagcccaa
    48241 cgaatgcagg aacaacataa acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg
    48301 aatcgctagc tttttgcaac atttgaagtc catctctcag ccattgaatc gaagaaccag
    48361 ccacaaaaat acttccttct aaagcatagt aaacctttcc atttatgccg taaccaattg
    48421 tcgtcaataa attatttttg gataattgcg gtttttcacc ggtattcatg acaataaatg
    48481 aacctgtacc ataagtattc ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg
    48541 cggcttgctg gtctcctgcc atccctgaaa ttggtgcttc gcttccataa aaatgataac
    48601 cttgcgtata accataaact tctgagttag aagtgacttt tggtaacatc actcgaggaa
    48661 tatttagtaa atctaaaatc tcctgatccc aatctagctg atggatattg aacaacatcg
    48721 tccgactggc attcgagtaa tcagtaacat gtgcttgtcc atcggtcagt ttccatacta
    48781 accaagaatc gatcgttcca aacatcaatt cgcctttttc agctcgttct tgcgcgcctt
    48841 ctacatgatc aagaatccag cgtatcttcg ttgctgagaa ataggcatcg atcactaacc
    48901 ctgttttttc gtggaaaaat tcttgatagc cttcattttt taagttatct gcaattccag
    48961 ctgactgtct ggattgccaa acaactgcat gatagattgg acgtcctgta tctttttccc
    49021 atattactgt tgtttcacgc tggttcgtta tgccaatccc agcaatctga tttggtttga
    49081 tccctgattc gatgaacgct ccggcaataa ctgactgtac agaattccag atttcatttg
    49141 catcatgttc cacccaactt tcttgcggga aatattgcgt gaactctttt tgagaactgc
    49201 cgatgtgtct tcctttcttg tcaaagataa ttgctcgtga gctagttgta ccttggtcaa
    49261 tcgccatgat atacgttgat tctgtcattg tgattccctc cattatatgc aaacgctttc
    49321 tttaactact cttatcatac tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt
    49381 ttttcttttt ttgacagttt tttattatga agctattttt taatcaattc ttttcttttt
    49441 tcagcgtctc tattcttctt ttcaatcgaa taatgtcgta aggtgacgca aattctgaca
    49501 gataatattc acactgtttt ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt
    49561 taccttcttc ataacttcca atctcaatac cgccgattcc cctcagttct ctgatcagat
    49621 attgttgaaa agcaatacga taaatcggca gactttctaa atcgattcca atcactactg
    49681 gttttgcaat ataaaaatcg atcatcaaaa gaacattggc atactggatc atcaggtaac
    49741 taagctcact atcttcagct cttttcctcg gcaattgctg caccgctgtt tcctgtaatt
    49801 tcgccagtaa ctgtgttaag gaatgcccga gcattttctg ctgctgttcc aaaagccgat
    49861 cacgctcata gacttcgatc tttccaacaa agaaaaacca ttcattgttg atctgggaaa
    49921 tttggtagcc tactgccttt tcttctttga ttttcaaacg acgaggacga taatgccata
    49981 acatcgtttc ccgaatgtaa gtatctaaaa cagcggttgg taactttttg ctccgtgtca
    50041 aatcatactg ataaaatatt tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa
    50101 acatcattgt ttcgtataca ttcagttctg ctgctgttcg actaagatgc atcacgatga
    50161 ttggatcgat tgcttgataa agccggtccg actgatagag taatttcttt tctttcaaag
    50221 ttgcgtacgt acttttttca ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa
    50281 tctgtgcttc tgctgatgcc gtaaacgttg tttctaatac acggtccaat cctagaatga
    50341 acggtctttt ttctggtgta ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt
    50401 ggaatagttc ataataaaaa tatcgtatct gtagctcttc tccgtataat tgaccgtttt
    50461 tgatttgtaa tccaaattcc tctaagagct ggttcaattc tttgatcttt cgaaaaacag
    50521 aagactcact gatattaaac gctgtcgcta atcgaacgat ggatacttgc tgatgttcta
    50581 ataaatagac gagcatctta aacttcaagc tttcctgcaa taaaaacaat agaatttttt
    50641 ccaaacttac atcttcgtcc aacacaagca gcgcctgata ctcatttcgc tgtacttcta
    50701 cttttttttc catcattcgg ccaatcaatt caatatcttc caaatattga tcaaacgagg
    50761 cttttgacag ttggacatgc tctctcaaac cattgataga ggctgttccc ccttgcagaa
    50821 caaggtttcg caaaatcagg atctgtaact gctcttttct ttctaataat gcttcaatct
    50881 tcataggcag tattcgccac ctttgaccac accatatctg gcacacggta ttctggctgg
    50941 aaaaatagct catgttcttc taactgttca aacgtccatc cttcttctac tgcaattgtc
    51001 agttggtcaa gaaggtggaa catctctttt ttgctttgga gctgtgctcc aagaatcttt
    51061 ttttcagaat caaatagaaa tttgagttta actttgcttt gatttattgc tgtaagcttg
    51121 tagtctacgt atcctgtttc aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa
    51181 cctacgcttc ctaagaaata gccaaaccac tgattgccga ctggacgata ggttccctga
    51241 tcctttgtct gtgagccact aattgtcttg ctgacatttc gtgctgtacg gacagcgtta
    51301 tttactagag aaacatacat tttttcattt gttggacgaa aagttacttg gatcgcatcg
    51361 ccaatcgcat aaacgtcttt ttgagacgtt tgcaagtagt catctaccca gatcgttcca
    51421 tcatcattca atgttaattg ttcttcccac atctgattgt cgggacgaga atgattagcc
    51481 agcaaaacaa gatcatagtt ctcctttctt ttttccgtct ctacaatagc ggtttcattt
    51541 tcggtcaaag acacaacttg ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc
    51601 tttactaacg gttctgtcat ttcaggatct agataacgaa aaagaattga ctttcggctt
    51661 tcgtaaagat ggatttgttt ttttcttgat gccaaccctt cagctatttc gattcctgct
    51721 tgtcctgcac caataattgc aattttcttt gcattcatga tcttttgctg aagagccgtt
    51781 acgttttgta tctttttaac tgggtgaata agcgttgatt ctgcagaaat atttcgaaac
    51841 ttttgatttg aaccagtcgc tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct
    51901 aaaacgactt ttttttcttt caatcctatt actgtctttt ctgtgtaaac atggattcga
    51961 tagcggtcag tcagttcttc tttcgtgatc caagaaagtt gtttttcact ggtaaccttt
    52021 ccttttaata tcaaattgaa cccgctggga atccatccta acttcgattg cttttcaatc
    52081 aaatatattt cacttgttgg catcaattgg cgaagataaa tagccgcatg aatccctgca
    52141 aacgacccgc cgacaattac aatgttcatc tgcattcctc tttcttataa ggtgttctac
    52201 tgctaacttc tcaatcagaa aacacagtat tcataactcg ctttcaacct tatgatacat
    52261 agcgatgcag cagaatcaac cctctccatc ataccgtgtt ctaggtctgc ttctcaataa
    52321 tgtactagtg gacctaattg aagagtatca tttctgaaga gatgtttcaa aaaatgtact
    52381 cttataaaag aaaaaaactc gccttttacg tacctctgtc caaataggaa acagaaaaga
    52441 acgaaaagac gagttttagc gtgtgtaatg taatagttag aatagacaca tcatttattt
    52501 ttaattcttt aatgcttcaa caactgcttc gcctaattga tgagcagatt gaggattttg
    52561 ccctgtaaga agccgcccat caatttgaac cacttctgaa aaagctgctc cagactgata
    52621 gtttgctcct ttttctttca aagcatcttc tgctaagaat ggtacttcat tcgttgtacc
    52681 atttgcttcc tcttcttcat tactgaaccc agtcactgta cgtccagcta aaatagattt
    52741 tccagactca tctgtaactg gcaacaatcc tacaacaccg tgacaaacag ctgagaccag
    52801 cccttcattt cgatagattt gtgaagcgac tttcccaaga tctattgctt ctggaaaatc
    52861 ccacattgtt ccgtggccac cagcataata aaccgctgca taatcatttg catccacatc
    52921 atcaggacgt aaggtatttc ccaaggcccg atttctgaaa tcttcatctt cataataggc
    52981 ccaatcgatt tcatccatat tcaaaacact ttgcggttct aaaggtacat atccgccttt
    53041 aggactgaca aaatctgcat cgatcccatt ttttactaat acatcataaa aatgagtcag
    53101 ttcacttaac cacaaacctg taggacgctc gatcttttcg tacttttcta catttgttaa
    53161 tacgatcaat actttcattt tcccatcctc tttctataaa aattgcgtta ttgataacgt
    53221 gctttcaaga aattatataa tgggcttggc aaataaagct ctttatatgc ttcttcaaaa
    53281 ggtttcgtta caaattcatc ctcacggttt tccaataatt tctctgccca gtcaggatcg
    53341 acgatcattt gctgtccaat tgctgcaagt tctgcatatt tcaatacctg ctctacatct
    53401 tcgcgattgc ggatcccacc gacaccaact aaagggatct ttccttgaat cgtgttatgg
    53461 accacttcaa gaatcgattt atcgttgtat ttatcagaac gagctattcg atcatacata
    53521 tttaatgaaa catgcagata atctagtttt gactcacgta atcgttcaag aagccagatc
    53581 gtgtcttcaa aacggatacc tggtgtttca tattcttctg gtgagaaacg ataccctacg
    53641 atgaatggtt ttttagcata ttgttcaacc gctgagaaaa ccgttgttaa taattcttca
    53701 ataaaacgat aacgattttc caagctaccg ccccattgat cctctcgtct attcgaatgc
    53761 ggagagaaaa attgttggat caaatacgta tttgctccat gcaactcgat tccatcaaat
    53821 cctgattcaa tcgctctacg tgtggctttg ccaaatgctt ggattgtttc aacgatttct
    53881 tcttcactca ttgctcgtgg tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca
    53941 gatacaggct gttctccaga taaagttgct cgacttgtca ttcgtccacc gtggaaaatc
    54001 tgaataatcg cttttgcgcc ttctccttgg attgctttcg ctagctcact taatcgagga
    54061 atcatttcat catgagcgat gctgagttca cctggccagc cttttccgat atcttggaca
    54121 tttgcagcac cagtaataac agcaccaaga cctttagaac gttgtttata ataagtgatc
    54181 tcatcttcgg tgatcactcc attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc
    54241 aatcggttct tcaattgaac tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt
    54301 gtcattcctc ccataattcg ctctgattaa cctatcgaga atcatctatc attaaaccac
    54361 tcgattagtt gtacatgcaa ctaatttgct taaaaaattt tatttttttt gtaacttatc
    54421 tgttgcatca ttcattgtct gggctaattc tttacttgct tcatttccca aaactgcttt
    54481 gtatgactga tgaagttctt cccagctacc atcgacttcc ttagcaaatt ggcgtccttg
    54541 ttctgtaaga taaactaatg acaagcgacc ttcgctaact gttttgacgt atcctttata
    54601 ttctaatttt tcaacaaaac gagcaatcgt tgatggggcg gaatagagca tttgtgcaat
    54661 atctttttgt ggtattccat ctttttcttt taatatcaat aaaataaaac cataagttgg
    54721 tgccagacct gtccttctaa acgcttcatc agctaattta ttcaacatgc gatctagctt
    54781 tttcacgcta aagaaaagac attcatctaa cgctttttca attggctttt ccatttcatc
    54841 ccctccattg gcgtacatat catagtatga tttttatttt ttgtcaaaaa acacggaata
    54901 atgtccgcct cctctgtcta gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg
    54961 cttgttttcg acaaaactcc ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt
    55021 tagttttgag tatgaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt
    55081 ggcttaatac cgaaaaactg gcttttgaac accgtttata tcagaggttg tgaaaaagcc
    55141 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt
    55201 ttgagctaat gccgatggtc agctgattgt acaccgttta ttgagaggtc gtgagcctgc
    55261 cgttgaactc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt tttatgacta
    55321 tggtgacttg tgatcgagga gttggcagga gaatactgtt tattcgtaaa gaaggggctg
    55381 tgacaagact tttgtcacag tcccttattt ccttttatta taggatttcg ccatttgatt
    55441 ggatgacttg tctgtaccaa tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc
    55501 cttcgtcatc ttgatcaaca tagatatatc cataacgttt tgacatttgt gaagtagaag
    55561 cgctgacgat atcaatgcag ccccacgacg tataaccaaa taaatctact ccatctttga
    55621 tagcttcctt catctgctca atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa
    55681 cggttccatc ttcttctact ttgtctatag ctccgagtcc gttttcagct atgaacagtg
    55741 gtttttgata cctatcatat aattgattca aagcaattct caaaccctta ggatcaatct
    55801 gccagcccca ttcacttgat ggaagatacg gatttttaat tcctccagtt attaagtttc
    55861 cttggacccc ttcccaattt tctggatgag cactgactgc actagacatg tagtagctaa
    55921 aagaaacaaa atcaacaggg taggctgcta agatttcttc atcacctact tctattttta
    55981 agttgacacc tttttcttca aacattcgtt gcgtatacgc tggataatat cctcttgctt
    56041 gaacatcact aaagaacaaa gaagttcttt gctgctcaat tgttttttga atatcatccg
    56101 gatcacaaga atacggaaca agaagctgat agctgatcat acaaccgact tgtgaacctt
    56161 tgataatttc atgacaagct tttgtagcta aagcacttgc tataaactga tggtgtgcac
    56221 cttggtattt cgcttgttcc aaatggggat ggttttcttc aattactcct acgctaacaa
    56281 aagggtgatg ttttacacag ttgatctcat tgaaagtcat ccaatatttt actttatttt
    56341 tatagcgttt gaatactgtt tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag
    56401 gccatccacc atattctgta accaaatata acggcatttc ataatgggaa agagaaacga
    56461 ctggctcaat cccatatttt agacattcgt caaacacttg atcataaaaa aataatcccg
    56521 cttcattggg tgtttcttca tcaccatgag gaaaaattct tgtccatgcg atagacatcc
    56581 gatagcattt aaaacccatt tcagcaaata aacggatgtc ttctttaaag tgatgataga
    56641 aatcatttcc gtgtctttta ggataataat acttgctgtt gggatcctgc gccgctttta
    56701 cttgctgatg cgtcatcata tgccattgat ctgtccattt ttctttaggt aaatcgctgt
    56761 cgaatgtata cacatcagac acagataatc ctttttcatc tacatcccat gcaccttcac
    56821 attgattggc cgctgtcgct ccgccccata aaaatccttc tggaaatttt ttcatatcga
    56881 tccccctaaa cttgctagtt ttcttgtttg attatttttc ttatgactag taatgatatt
    56941 tttgaatcta ctactatttg taacaataac cattgtggtc ggatcatatc cctctttgat
    57001 taattcttgc aaatctactt ccatcaataa atcgccttta cgtacttttt ttccatcaga
    57061 tactgcactt tgaaagtatt tgccatttaa attcactgta tcaataccga tatggatcag
    57121 tatctctgct ccttcttcac tgactacccc aattgcatgt ttcgttggaa aaactgttga
    57181 aacttccccg tttacagaac tgtagatttt tccgtccgtt ggaatgatcg caaacccttc
    57241 tcctaaagat ccactagcaa atgttggatc gtttacttca gataaatcaa ttatttcacc
    57301 tgctgcagga gtacttaaca ctatatcctt tgaggattct tcttcgattt catccttgta
    57361 gccgaaaaga aaagttagca tacaggaact gatcatcgct aagaaataac tagcaactag
    57421 ccaaacaaat ccttcaccaa tataaatcgg gaaagacaaa atacttcttg tagcaactgc
    57481 caaagcaccg gctcctgcgg caccagctac tccaccaaat atagctccac atatacaagc
    57541 aatgtaaaat ggtcgtttat atttaagagt cacaccataa acagcaggtt cagtgattcc
    57601 aaataatgct gtgataaatg cagataaaga taaacttttt actttcttat ttttcgtttt
    57661 caaaaatacc ccaagtactg ctcctgcttg cgcagctacg gcagtcatac atgctggacc
    57721 tagcgtatca cgaccgtaga aagaaagatt gttggtcatt actggtacaa gtccccaatg
    57781 taacccaaaa atcactaaca cttgccaaaa tccgccgata acagcaccag ataaaacagg
    57841 gctaaagtta taaacagcta aatatccagc tgcaatcagc tgacttgcat aatctgcaat
    57901 tggacctaca actaataaaa taattgggac catcacgatg atacaaatga atggtgtcaa
    57961 taaatttcga atactttcgt gcaagctttt gcgaatctta ggctcaatat aagaaagaac
    58021 ccatacagct aaaattgctg gaataaccgt acttgtatac cttgctagaa tcacgggcat
    58081 ccctagaaat cgcaaggtta tgctatcact atatgcactg acaatcgtag gataaacgag
    58141 agctgctgca ataaccatag cgatgaaacg atctgtattg aacttttttg cagcagtata
    58201 tgccaaaaat ataggcaaga aatagaaaaa tgcatcagct gctgcattca aaatgatata
    58261 agttcctgat tcagtagtta ataaattcaa actcgtacat aaggcgagta ctcctttgat
    58321 caaaccactt cctgccaaga cgcctaaagt cggggcaaaa acagcggcga tcatgtctat
    58381 aaaagagttt aaaatgcctt ttttctcagt tcctctcttt ccactagatg catcatcttc
    58441 tagtttcatt tgtttggata atacgtcata cacttcattt actgcatttc ctactacgac
    58501 ttgaaacatt cctccagctt cgactacagt gatcacacct tctagttgct ctaccttctc
    58561 cttatctgcc ttcttattat cttttagggt aaagcgtaac cttgttgcac aatggtaaac
    58621 attgctgaca ttttcttcgc ctccgacaaa tcggacaata tctttcgcta attgtgaata
    58681 gtccattttt acttccccct tgttaattaa tgccgtagca atccttctta caagataagt
    58741 atagttctta gaaaacggtt tctccattca attagtaccc cttctaatag cgaacgaaac
    58801 tgtacaatag tgtgtatggt ttacttagcc gagcaaatca ctcactgctt gaaagaaaac
    58861 aagtatatcg tttctcttat agtcaaagga gacaaaaaaa acacgcttga tcttcctttg
    58921 ctgatgtata agtggtatct caagctgata ctctgtgatt tttgtttcta cttggctttt
    58981 gataagacag cacaacaaag tctcggcatt taacggaagc ataactaggt cagaactagg
    59041 agagattagt tgctgaatgg aatctatctt tttatggatt tttatttgat tttcttcttt
    59101 gtagtaatga tcaagcagtt ttgttttctt tttttcttca actatggtca gcttttcacg
    59161 caaaatctga taaatctccc aagattcaaa ctcatctaga acactaggta gataaatcaa
    59221 atcatcccac tcaaaaataa gctcttgctc agtagtcaaa attgcatcat attccgttat
    59281 tttgtttgat tgatttttga aaagatacac tggttctatc cggcattctt tgatccacat
    59341 gttcatactt tgttgaatct gtttttttag tgtattcaaa atactaattg gctggttact
    59401 aacaatcagt atctcccctt gttttcgaaa aggattcaac gcttcggcaa gatacaaaac
    59461 aaaataagcg ctttcagcta aattgggacg taattgaaaa tgtattggga agaaacgccg
    59521 aattaaataa atttctaaag gataacggga gatgatcttt tgtacaaaat gattcatggt
    59581 attatgtccg gcatccatcc tcgttttcat ttgtttgata tgggttagca aaaattctgt
    59641 ttcatggaac aataacgaaa caggaagatc taaaaaacga ataatcgttt gctcaaatgc
    59701 tcttagattc ttctctattt ctttgttttt ttcatcatta atcatcaaat aatttaactc
    59761 taatagtcga ttttcaattg cttgtttttc ttgattggaa aaagaatagg gtagttctga
    59821 tgataacttt tttgtgaggg tctctaccat cggaataaat aatgtatacc tgtttgtttt
    59881 ttctaaaagt tcttctcttc gaagaatggt caaaacgaga taccgagaaa atcgtatgaa
    59941 gtcctctcct gaaacaatat aattatatga tataagaacc tcttttaatg tcttttggac
    60001 agtcggcatg atattggaaa aaattgtact gatttgttga ggaaaccttg cgtgttggat
    60061 caccgtttct gttccaacaa gagataagaa aattcttttt gaattttctt cagcaatgat
    60121 ttttagtcct tttgtcgacg aaacttccag ttctaattta ggattgcgct cgatccaacg
    60181 caaaatcgtt tttatttgta atgatacggc tgtttttgaa agaaagaaaa tgtctgctat
    60241 cttttgcata ctgacatacc ctttttgaaa caataagtac gtcacaatag ttgaagctcg
    60301 gaaatccatg ctggaataga ttttatgttt cgtcacctct tgtatgattt gctgtttagt
    60361 ttcagattca attgattcta aaaaataacc tgaacgttta tttgactgga taatgatttt
    60421 cttctcgtag atagcaagca tatttttgat ttcagtttgg atcgttttct tgttggtacc
    60481 taaagcttca gctaattctt cgcttgttat ccaatgattc ttatttctta atactacaag
    60541 aatttggagt tgtcgtttat ttaagtccat ttgcttacac ctcctctttg ttataaacat
    60601 acaggaaaaa cctctcaaat gacaatagtc acttgcagag gtttaaaaat catttgaaga
    60661 aagatctata ttcaaggttt ttaccattag actggataat ttgtttataa tattcaaaac
    60721 tatctttttt caatctccta aagcttccgc tcccatcatt ttgtctatcc acataaataa
    60781 agccatatcg cttcctcatt tcccctgttc ctgccgatac gatatcgatt ggtccccacc
    60841 ataagtaacc aatgatttgg caaccatctt ttattgcctc agcaatttcc tgaagatgta
    60901 tttttagata ctcacaacgt tttggatcgt ggattcgatt caattcatct ggtgcttcat
    60961 ctagtccaat gccattttca actatgaata atggtaattc ataacggtct gtcaattcat
    61021 tcatgacata acgaaaccct agaggatcaa ttggccaact ccaaggttcg ggagattttt
    61081 ccttcaaaaa gggattatcg aaaccatttg ctccgccagt atctacgcgc atttctgctt
    61141 cttttttata gacagcgcta cgataataac taaatgcaat ataatctgcc gtgttagctt
    61201 gcaagcactg ttcgtctcca tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc
    61261 tgtaaatata agctggataa tgccctcggc acatgacatc ggagaaaaac catgaattgc
    61321 gtttaaattg cagtgaacca aatacattct ctggatcaca gtcatatgca taagtcgcta
    61381 atgcagaaaa gctacacata atacccatct tagcatttgg atcgattttc tttaataatt
    61441 gagtagcacg ggcgttcgct agaaacatat gatgacaggc ttgataaatc actttttttg
    61501 ttagtgtagc aatgggttca agtttatctc tagcagggaa tgtaaccaat ccagcagctg
    61561 cataaggcat acgaaaagca ttattgatct cattaaaggt catccaatat ttcactttcc
    61621 cttggtagcg ttcaaagacg gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat
    61681 tttgccaccc cccatactct cctaccaatg ctaagggggt ttcataatga ctaagagtaa
    61741 ttactggctc gatatcatat cgatttaatg tattgattag tctgtcataa aatacgagac
    61801 cagcctcatt tggcttctgt tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg
    61861 ttcgaaaagc ttttaaacct aactccttca gtaaacgaat atcctcttca aatcgatgat
    61921 aaaaatcaat cgcttcatga gaaagataat agagtgattc gtctaaagca atctcccaaa
    61981 tctttttttc ttcattccac tgaatactag gatgctggtt catgatcccg ataagcgtat
    62041 ctgtcacatt aggtaatttg ccgtcttcca accatgcacc ttcacattga ttagcagcta
    62101 ctgctccgcc ccataaaaaa ttttctggaa atatttcctg atccataact atctctcctc
    62161 gctatcaaca atttcttact tgaagtatag ctcgattcat caaaacaaga tattctaact
    62221 atttcggcag aagagaagaa aaaaattgaa tgctactctt catttttaat atttatatat
    62281 aacaaataca taagacgatc cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt
    62341 tattttactt ccaacacaaa tgcttcataa ggtcgtaaat caactttgcc gtctggtaac
    62401 tgtttcagag gataatttga cacaataacc cttaaagctt cacgtggaat agtgaacgtc
    62461 tgcttttcac tggacatatt acaaataacg agccattgct ggtcttcata tatgcgagaa
    62521 taggcaaaga catgctcagg cgtcttttcc actaattcat agtctcccca gacgatgatg
    62581 tcatgttttc tacgcaaatc gatcaatttc ttgtacatat aaaaaacaga atcttgatct
    62641 gctaacgctt cttctgcatt gatttcttta taatttggat tgactcgaag ccatggaatt
    62701 ccagtagtaa acccagcatg ttcacgggcg ttccattgca taggcgtcct agcattgtct
    62761 cttcctttcg ccttgattga ttccaagatt tcctcttttg taaatccaga agaaatcctt
    62821 tcgttataca tattgattgt ctcaatatct tttgcttccg cgatatcatc gattgggcaa
    62881 ttcgtcatgc ctaactcttc tccttgatag atataagggg tgcctttcat catatggagc
    62941 aagatagcaa acatcttggc actttctttt cgatacacat cgtcatttcc ccaacgagaa
    63001 acaattcgcg gaagatcgtg attattccaa aataaactat tccatccttc atcacctaaa
    63061 gatgtttgcc atttggataa tacttttttc agttcagaga tactcaaggg tttcaaatcc
    63121 catttgtctt tgccttcttg ctgatctaat cctatgtgtt cgaattggaa gaccatggat
    63181 aattcatttc ttttaggatt ggaataaagt ttagcgattt cggtagttgc tccccatgtt
    63241 tcgccaacag tcatcagatt tttgtcacca aaagttgctt gattcatttc ctgaagatac
    63301 tcatgcagtt taggtccatt acttgtgatt tctttatccg gttctttgcc aatcatatcg
    63361 atcacgtcca tacgaaagcc cccaatacct ttatcgatcc agaaattcat catttgataa
    63421 atctcttggc gaacctgttt atttttccaa ttcaaatcag gttgacgctt gctatacaag
    63481 tgcagaaaat attgccccgt tttttcatcc aactgccaag cagaaccact gaatattgag
    63541 cgcaatctat taggctcttt tccattgacc ggatcacgcc aaatataata gtcccgataa
    63601 ggattttctt tatttttacg ggcctcaatg aaccacggat gttcatcaga agtatgattc
    63661 accaccagat ccatgatgat acggatattt cttttctttg cttcttcaat cagttcttcc
    63721 atttcttcca tcgtaccgaa ttctgccatg attgcttgat aattactgat atcataccca
    63781 ttgtcatcat tcggagactc gtatactgga gaaagccaaa ttgcatcaat acctaaatac
    63841 gccagataat ccaacctgga aataatcccc cgcaaatctc caataccatc ttgatttgaa
    63901 tcttgaaaac ttcttggata gatttgataa acaactgaat ggtgccacca tttttgttcc
    63961 atgattttcc tcctataaat ggatgtttct attttttgct taatcattta ttttagtctc
    64021 tctcactaaa aattgtaacg ctttcaaata gctattgcac cttgttatcg gtaacagaat
    64081 aaaacaaaaa aacagctgag gaaattccct caactgtttt ttgaaaggat tgatttatgc
    64141 agggaagaat gctgatttca caacttcttt ataaacggtg ttcactcagc tgtcccttgg
    64201 tattagctca aaaaacggca aaatataaga aactattttt tcgttttttt ctctaatact
    64261 caggccagaa cgctgatttc acaacctctt tatttcacat tcaatatcca catgaatccc
    64321 acaaatacaa agaacagacc atacatgatt ggagatactt cttttcctct tttagctgct
    64381 aacatggtaa ttggatagaa gataaaacct aatgcaatcc catctgaaat actataagtc
    64441 aaaggcatgc cgatcaagat caagaatgaa gggatcgcaa tctctaagtt tccccattcg
    64501 atttgtcgca aagattgtgc catcaatact cctaccataa tcaatgctgg tgcagttact
    64561 tgtgaagtaa caactgatag taatggagaa aagaacaagc ctaacaagaa gaaaatccct
    64621 gtagtaatag ctgtgatacc tgaacgtcct ccaacggcga taccagctga tgattctaca
    64681 aatgcaccaa caggagaagt accaaataag gaacccgcaa gcatagctgt tgaatcagaa
    64741 gctaaagctt tccctacacg aggcatcttg ttgtctttca tgaaacctgc ttggtttgct
    64801 aatccaacta aagttcctgc tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg
    64861 acccacattt gaagagaatt gatgtcccct acatgtttta atgcaactaa aaatgttggt
    64921 ttcaaacttg gtgctgcggc aacaatttca gaaggcattg gaatcaaacc tgtgaccaga
    64981 cctaaaatcg ttgtagctgc catcccgatg aagatgccac ctggtacacg ccgaacaagc
    65041 aagatagcag ttacgactaa tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat
    65101 cctaatccta ctaatgtaga ttcattcgaa acgatgatcc caccttcgct cagaccaagg
    65161 aaagcgataa atagaccgat accgccagaa atcgcatatt tcaaatctgc tgggatcgca
    65221 tcaatgatca tttcccgcag cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg
    65281 aatacgccgg ctaatgctgt ttcccatgga atccccattc ctaaacaaac agaataagca
    65341 aaaaaagcat tgatcccaag agcaggtgcc gttccaattg gataacgggc taataccccc
    65401 attaaaacac agccgatcgc actggctaat gcagtcgcag taaacactgc tccttcatcc
    65461 attccagaag cacctaatac agttggattt acaaataaga tataagccat tgagataaat
    65521 gttgtaaatc ctgccagtac ctcacgtttt accgtggtgt tcaatttttc tatttcgaag
    65581 taggaaagta ttttttcctt catcttccat ccccctaaat ccgaacatta taaataaagt
    65641 ccgttaaaat ttcgatttat taataaaaca acgtgtttta tcatatacta aaacaaaaca
    65701 taagtaaaca aaaaattcgg attttaagca aaatccgaac gttccatctg attaaatccc
    65761 tttatctcaa agttcatgaa cattcacttt ggattacaag aatttcttta ttgtgttaat
    65821 aaagtttctt cattctagtc tcaaaaatca atagtgtgat ttgattttac catttcgttt
    65881 ctgttcttct ttgatgaatt cttttgtatc gacaggattc agattattta gtccgccaaa
    65941 acctgtatcg accatttttc cccagccttt tgtcttcttt tttcctgatt tgagattctt
    66001 tgctcgaagc ttctcactat gttttgctcg ccgatacttc aaataacctt ctaatcttcc
    66061 cattagatcc actgtttttc ctccttttta ttcaatcaga cgactcagct tttcctataa
    66121 caaaataatt gccttcaggg tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga
    66181 tttctcctgg ttcttccatg ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta
    66241 aagaaggcgg tggtcctaat acctctggtg aaaacttctg cacaaatact tttgggaata
    66301 cgcctaattc taattctttt gagatgctga gtacaatact cttaaattct tctggcaatt
    66361 cgattgtttc agaaatttct gcaccaattt tttctacaaa gaatcggcaa atcatctcta
    66421 cgtcattgac ataaagcatc acacgtgttt tttgtatcat catttgaccc ttctttctat
    66481 ttgattttct ttcctgtcta taaactgatt ccattatatc acgaaagcgc aaacaccttg
    66541 tgtttgatac tattcaagaa aaataaagca atagtttcta ggattgcttt cctccacaaa
    66601 ctttttttac aaaaaaaaaa cggcaatgaa tgccgctctt cttatttcga ttcttttata
    66661 attctgcttc tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt
    66721 gatcgatttt ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat
    66781 tgatggattc aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg
    66841 cttcacgcca gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt
    66901 ccggaagtgc cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg
    66961 cttttcctaa atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc
    67021 caaaagacaa ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc
    67081 gttcttgatt tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct
    67141 tgaatgtggt aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt
    67201 ctgtaaggta atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt
    67261 cactttcttt tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt
    67321 caggaaatac ttcccagaaa ttggtatcca ctattacttt tttcatctgt tcacacctct
    67381 tgattagaat aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa
    67441 aacatgttcg ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt
    67501 gtgttcccaa acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc
    67561 tgtatctagt aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg
    67621 ctcgatttct aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt
    67681 caggtaaccc gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt
    67741 tagctcttct gtaagttttt ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc
    67801 cggtgaatta tcttttccaa atgtatctgt aaatgtttcg atactaattt gttgtagtaa
    67861 gtctacatct gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca
    67921 atagatcctt ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac
    67981 tttatttaaa agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac
    68041 tgcattggag tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt
    68101 cggatagagt ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt
    68161 ggattctaat ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc
    68221 tagtttttct tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc
    68281 tcttgttaaa tcgacttctt tgaattcgat attgctaata gaatctagcg ctcgggcaat
    68341 cattccgatg tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac
    68401 tagttcacct ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt
    68461 taataactag taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc
    68521 tggtttttgt tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt
    68581 ggcaactctt ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg
    68641 gcctcttcac cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga
    68701 atatcttttt gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat
    68761 cctgtttctt catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca
    68821 aatccaccag gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca
    68881 tcttttttga tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt
    68941 gtgagcgctt ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa
    69001 atcgtccgga tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca
    69061 gcttgagaaa gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct
    69121 tcttttttag caaatgtatg actatgtaat caagtataga agactggttt aattacggca
    69181 aataaatata atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga
    69241 cattcgactt ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata
    69301 agccccacca aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag
    69361 gcaggacagc tttttcacaa cctccccact aaacggtgtt ctcctaccaa ctcctcggtc
    69421 acaagtcaca atattcatca aacatgagaa gctgtttttt tgaaattgct ccttgtgact
    69481 cggagctgaa cgacaggctc acgacctctc ggtaaacagc gttcaaaagc caatttttcg
    69541 atattaagcc atttctttgt tacttattgt cattgatttc tttttctaat ttatccaata
    69601 gtggagtgta atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact
    69661 ggaatttgtc atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat
    69721 tggtcccttt cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc
    69781 tcaaatgacg atctgattca cgatcacgtt tgtaccgacg ttgccataac acttcaaagt
    69841 ctgctgttag gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc
    69901 gtgctttttg cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca
    69961 tatacaaatc aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc
    70021 gttcttccaa attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg
    70081 tgatggcata catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg
    70141 taccaggact accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta
    70201 attggacttt ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct
    70261 tcatttgctg gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt
    70321 tctttagcta ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc
    70381 ccgttattga tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact
    70441 agggggacat caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt
    70501 gatttgaatt gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct
    70561 tctacaaatt tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg
    70621 attccttctt ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca
    70681 tgggcatatt ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac
    70741 ccatcataca tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct
    70801 cgggcgtggt ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt
    70861 acattgacta acacatgcat gccgatatat ttagcggtat cgacacttgt ttgaataatg
    70921 atcggcgaac ccatttcttt tgctgcgcct agcatagcag gaagcatttc taggttatgc
    70981 gtattaaaag ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat
    71041 gttgtataca tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg
    71101 ttttgcaatt tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc
    71161 accagcacga gcagcatctg cacgcttctg gttatagagg ctcatcagtt ttttataccc
    71221 atctccgatc gtctgtttat tttccaaact cttttctaaa tattggatgg cttgatcgtt
    71281 cacatacaat tcagaaaatt taagtccaac ttgttcataa agtttcgcta atttatgctt
    71341 atcttgggta gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt
    71401 cgcttctggc gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt
    71461 tttcttaaaa aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg
    71521 gcttaagatc caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc
    71581 attgttgaat cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa
    71641 gaaaccatga gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa
    71701 tatccctgct gtccctccgg caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa
    71761 gccgaacatg ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc
    71821 taaaatgaca gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc
    71881 atcaggaact aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag
    71941 acgtacacca gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc
    72001 aaacatgata tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa
    72061 aacagtgatg atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag
    72121 aaattcgaaa tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc
    72181 accaaaaagc ttagcaacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa
    72241 agcaatatca tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc
    72301 aatggcgaaa caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc
    72361 ggcaaaatag ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccagttaa
    72421 aaaaatgtac ttccattttg tgaaacgagc caaaataata ttgaatacaa aaatggctaa
    72481 gaacgtcagt gcaatatctc tacctaaacc caaatcattc attgcttgac cattgataga
    72541 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    72601 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    72661 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    72721 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    72781 aacgcccatg tt
//
LOCUS       DACIWG010000007        66819 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.007, whole genome shotgun sequence.
ACCESSION   DACIWG010000007 DACIWG010000000
VERSION     DACIWG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.007"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            212..1018
                     /gene="proB"
                     /locus_tag="IYF54_000447"
     CDS             212..1018
                     /gene="proB"
                     /locus_tag="IYF54_000447"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ9830809.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1015..2262
                     /locus_tag="IYF54_000448"
     CDS             1015..2262
                     /locus_tag="IYF54_000448"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ9830810.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2338..2682
                     /locus_tag="IYF54_000449"
     CDS             2338..2682
                     /locus_tag="IYF54_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830811.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2799..3125)
                     /locus_tag="IYF54_000450"
     CDS             complement(2799..3125)
                     /locus_tag="IYF54_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830812.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3498..4699)
                     /locus_tag="IYF54_000451"
                     /pseudo
     CDS             complement(3498..4699)
                     /locus_tag="IYF54_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4816..5331
                     /locus_tag="IYF54_000452"
     CDS             4816..5331
                     /locus_tag="IYF54_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9830813.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5530..5976)
                     /locus_tag="IYF54_000453"
     CDS             complement(5530..5976)
                     /locus_tag="IYF54_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ9830814.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6023..7501)
                     /gene="msr"
                     /locus_tag="IYF54_000454"
     CDS             complement(6023..7501)
                     /gene="msr"
                     /locus_tag="IYF54_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9830815.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7855..8484)
                     /locus_tag="IYF54_000455"
     CDS             complement(7855..8484)
                     /locus_tag="IYF54_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9830816.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8468..8932)
                     /locus_tag="IYF54_000456"
     CDS             complement(8468..8932)
                     /locus_tag="IYF54_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9830817.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9419..10687)
                     /locus_tag="IYF54_000457"
     CDS             complement(9419..10687)
                     /locus_tag="IYF54_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ9830818.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10918..11655)
                     /locus_tag="IYF54_000458"
     CDS             complement(10918..11655)
                     /locus_tag="IYF54_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9830819.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11722..12291)
                     /locus_tag="IYF54_000459"
     CDS             complement(11722..12291)
                     /locus_tag="IYF54_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ9830820.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12419..13291
                     /locus_tag="IYF54_000460"
     CDS             12419..13291
                     /locus_tag="IYF54_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9830821.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13633..14505
                     /locus_tag="IYF54_000461"
     CDS             13633..14505
                     /locus_tag="IYF54_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9830822.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14502..16460
                     /locus_tag="IYF54_000462"
     CDS             14502..16460
                     /locus_tag="IYF54_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830823.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16711..17562)
                     /locus_tag="IYF54_000463"
     CDS             complement(16711..17562)
                     /locus_tag="IYF54_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9830824.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17685..18152
                     /locus_tag="IYF54_000464"
     CDS             17685..18152
                     /locus_tag="IYF54_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ9830825.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18415..18897)
                     /locus_tag="IYF54_000465"
     CDS             complement(18415..18897)
                     /locus_tag="IYF54_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9830826.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18997..19860)
                     /locus_tag="IYF54_000466"
     CDS             complement(18997..19860)
                     /locus_tag="IYF54_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9830827.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20458..21306
                     /locus_tag="IYF54_000467"
     CDS             20458..21306
                     /locus_tag="IYF54_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830828.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21477..21818)
                     /locus_tag="IYF54_000468"
     CDS             complement(21477..21818)
                     /locus_tag="IYF54_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ9830829.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21904..22611
                     /locus_tag="IYF54_000469"
     CDS             21904..22611
                     /locus_tag="IYF54_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ9830830.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22598..22921
                     /locus_tag="IYF54_000470"
     CDS             22598..22921
                     /locus_tag="IYF54_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9830831.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23010..24047)
                     /locus_tag="IYF54_000471"
     CDS             complement(23010..24047)
                     /locus_tag="IYF54_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9830832.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24494..25477)
                     /locus_tag="IYF54_000472"
     CDS             complement(24494..25477)
                     /locus_tag="IYF54_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ9830833.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25893..26663)
                     /locus_tag="IYF54_000473"
     CDS             complement(25893..26663)
                     /locus_tag="IYF54_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830834.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26656..27591)
                     /locus_tag="IYF54_000474"
     CDS             complement(26656..27591)
                     /locus_tag="IYF54_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830835.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28185..29834)
                     /locus_tag="IYF54_000475"
     CDS             complement(28185..29834)
                     /locus_tag="IYF54_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ9830836.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30029..30925)
                     /locus_tag="IYF54_000476"
     CDS             complement(30029..30925)
                     /locus_tag="IYF54_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9830837.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30918..31598)
                     /locus_tag="IYF54_000477"
     CDS             complement(30918..31598)
                     /locus_tag="IYF54_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9830838.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31599..32381)
                     /locus_tag="IYF54_000478"
     CDS             complement(31599..32381)
                     /locus_tag="IYF54_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9830839.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32374..33291)
                     /locus_tag="IYF54_000479"
     CDS             complement(32374..33291)
                     /locus_tag="IYF54_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9830840.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33294..33578)
                     /locus_tag="IYF54_000480"
     CDS             complement(33294..33578)
                     /locus_tag="IYF54_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9830841.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34038..34430)
                     /gene="rpsI"
                     /locus_tag="IYF54_000481"
     CDS             complement(34038..34430)
                     /gene="rpsI"
                     /locus_tag="IYF54_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ9830842.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(34446..34889)
                     /gene="rplM"
                     /locus_tag="IYF54_000482"
     CDS             complement(34446..34889)
                     /gene="rplM"
                     /locus_tag="IYF54_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ9830843.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(35408..35875)
                     /locus_tag="IYF54_000483"
     CDS             complement(35408..35875)
                     /locus_tag="IYF54_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ9830844.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(35995..37533)
                     /locus_tag="IYF54_000484"
     CDS             complement(35995..37533)
                     /locus_tag="IYF54_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ9830845.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            37613..38299
                     /locus_tag="IYF54_000485"
     CDS             37613..38299
                     /locus_tag="IYF54_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ9830846.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(38500..39027)
                     /gene="msrA"
                     /locus_tag="IYF54_000486"
     CDS             complement(38500..39027)
                     /gene="msrA"
                     /locus_tag="IYF54_000486"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9830847.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(39111..39788)
                     /gene="pgmB"
                     /locus_tag="IYF54_000487"
     CDS             complement(39111..39788)
                     /gene="pgmB"
                     /locus_tag="IYF54_000487"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ9830848.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(39785..40915)
                     /locus_tag="IYF54_000488"
     CDS             complement(39785..40915)
                     /locus_tag="IYF54_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9830849.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(40915..43098)
                     /locus_tag="IYF54_000489"
     CDS             complement(40915..43098)
                     /locus_tag="IYF54_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ9830850.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(43095..44120)
                     /locus_tag="IYF54_000490"
     CDS             complement(43095..44120)
                     /locus_tag="IYF54_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9830851.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(44165..44992)
                     /locus_tag="IYF54_000491"
     CDS             complement(44165..44992)
                     /locus_tag="IYF54_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9830852.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(44989..46056)
                     /locus_tag="IYF54_000492"
     CDS             complement(44989..46056)
                     /locus_tag="IYF54_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ9830853.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(46082..46918)
                     /locus_tag="IYF54_000493"
     CDS             complement(46082..46918)
                     /locus_tag="IYF54_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9830854.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(46915..47784)
                     /locus_tag="IYF54_000494"
     CDS             complement(46915..47784)
                     /locus_tag="IYF54_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9830855.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(47854..49137)
                     /locus_tag="IYF54_000495"
     CDS             complement(47854..49137)
                     /locus_tag="IYF54_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9830856.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(49151..50836)
                     /locus_tag="IYF54_000496"
     CDS             complement(49151..50836)
                     /locus_tag="IYF54_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ9830857.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            51111..52136
                     /locus_tag="IYF54_000497"
     CDS             51111..52136
                     /locus_tag="IYF54_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9830858.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(52731..56384)
                     /gene="rpoC"
                     /locus_tag="IYF54_000498"
     CDS             complement(52731..56384)
                     /gene="rpoC"
                     /locus_tag="IYF54_000498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ9830859.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(56463..60080)
                     /gene="rpoB"
                     /locus_tag="IYF54_000499"
     CDS             complement(56463..60080)
                     /gene="rpoB"
                     /locus_tag="IYF54_000499"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ9830860.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFVDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            60808..61776
                     /locus_tag="IYF54_000500"
     CDS             60808..61776
                     /locus_tag="IYF54_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9830861.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            61794..62699
                     /locus_tag="IYF54_000501"
     CDS             61794..62699
                     /locus_tag="IYF54_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830862.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            62696..63502
                     /locus_tag="IYF54_000502"
     CDS             62696..63502
                     /locus_tag="IYF54_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9830863.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            63659..64627
                     /locus_tag="IYF54_000503"
     CDS             63659..64627
                     /locus_tag="IYF54_000503"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ9830864.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(64944..65468)
                     /locus_tag="IYF54_000504"
     CDS             complement(64944..65468)
                     /locus_tag="IYF54_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ9830865.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            65755..>66819
                     /gene="scm"
                     /locus_tag="IYF54_000505"
     CDS             65755..>66819
                     /gene="scm"
                     /locus_tag="IYF54_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ9830866.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        19354 a        14204 c        12266 g        20995 t
ORIGIN      
        1 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
       61 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
      121 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
      181 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
      241 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
      301 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
      361 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
      421 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
      481 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
      541 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
      601 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
      661 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
      721 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
      781 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
      841 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
      901 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
      961 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
     1021 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
     1081 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
     1141 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
     1201 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
     1261 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
     1321 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgaccggcat catttacgaa
     1381 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
     1441 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
     1501 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
     1561 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
     1621 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
     1681 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
     1741 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
     1801 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
     1861 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
     1921 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
     1981 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
     2041 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
     2101 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
     2161 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
     2221 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
     2281 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
     2341 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
     2401 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
     2461 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
     2521 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
     2581 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
     2641 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
     2701 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
     2761 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
     2821 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
     2881 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
     2941 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
     3001 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
     3061 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
     3121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
     3181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
     3241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
     3301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
     3361 caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     3421 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
     3481 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
     3541 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
     3601 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
     3661 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
     3721 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
     3781 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
     3841 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
     3901 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
     3961 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
     4021 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
     4081 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
     4141 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
     4201 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
     4261 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
     4321 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
     4381 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
     4441 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
     4501 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
     4561 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
     4621 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
     4681 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
     4741 gtaaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
     4801 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
     4861 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
     4921 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
     4981 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
     5041 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
     5101 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
     5161 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
     5221 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
     5281 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
     5341 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
     5401 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
     5461 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
     5521 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
     5581 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
     5641 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
     5701 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
     5761 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
     5821 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
     5881 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
     5941 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
     6001 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
     6061 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
     6121 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
     6181 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
     6241 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
     6301 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
     6361 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
     6421 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
     6481 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
     6541 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
     6601 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
     6661 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
     6721 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
     6781 ccggtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
     6841 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
     6901 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
     6961 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
     7021 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
     7081 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
     7141 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
     7201 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
     7261 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
     7321 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
     7381 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
     7441 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
     7501 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
     7561 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
     7621 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
     7681 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
     7741 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
     7801 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
     7861 gaacgtagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
     7921 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
     7981 caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac
     8041 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
     8101 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
     8161 tggcatcttc cctgattcgt aagcttggtt atagatttct tcgccatatt cgtaacattg
     8221 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
     8281 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
     8341 ttgcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
     8401 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
     8461 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
     8521 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
     8581 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc tcgattttct
     8641 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
     8701 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
     8761 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
     8821 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
     8881 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
     8941 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
     9001 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
     9061 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
     9121 ttattgattc ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc
     9181 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
     9241 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
     9301 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
     9361 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
     9421 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
     9481 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
     9541 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
     9601 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
     9661 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
     9721 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
     9781 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
     9841 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
     9901 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
     9961 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    10021 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    10081 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    10141 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    10201 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    10261 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    10321 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    10381 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    10441 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    10501 aaattgtatt ttctgagaca gtagctgact gcaaagtact ctctgtatcg ttttcaattc
    10561 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    10621 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    10681 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    10741 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttctttttg ataaatttgc
    10801 tttttctaca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    10861 tggtcgtttg aataactatt taacaagtaa ttctctttgg atattatccg ttatggatta
    10921 tacttcctgt gtcgtcggac ggataatcat gttactgaca gacatccggt ctggcgtttc
    10981 tatcgcaaat gctacagcag aagctatatc ttcggaggtc aatccccatt ctttttgcgc
    11041 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    11101 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    11161 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
    11221 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    11281 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    11341 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    11401 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    11461 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    11521 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    11581 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    11641 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    11701 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    11761 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    11821 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    11881 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
    11941 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    12001 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    12061 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    12121 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    12181 cgctgctaaa ggctcttcgt tttcagcttg gatctgatgg atcacttcaa agagcggatc
    12241 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    12301 tccttttatc cttactttat aacggacggc ttatcatgtc taatacttat aaaacatact
    12361 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    12421 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
    12481 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
    12541 agaacttggt gtgacacttt tcactcgggg ctcccgagag atacagttga cggattctgg
    12601 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
    12661 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
    12721 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
    12781 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
    12841 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
    12901 ccaagatcgt tggggacttt tgatacatcg aaatcatcct ttagcaaagc aaaccgtcat
    12961 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
    13021 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
    13081 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
    13141 tgggatcatc aatacttatg ggacggaaaa aatattcatc ccactcgatc cgccattaca
    13201 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
    13261 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
    13321 tttaacaacg aataagataa gcaaaagatc caacgattaa ataacataag tttcttaatt
    13381 tatttcttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
    13441 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
    13501 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
    13561 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
    13621 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
    13681 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
    13741 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
    13801 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
    13861 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
    13921 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
    13981 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
    14041 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
    14101 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
    14161 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
    14221 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
    14281 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
    14341 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
    14401 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
    14461 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
    14521 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
    14581 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
    14641 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
    14701 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
    14761 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
    14821 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
    14881 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
    14941 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
    15001 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
    15061 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
    15121 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
    15181 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
    15241 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
    15301 aaaacaaagc aaaaacagct tccttgatgc tagcagcagc ttttgcttca ttctttactg
    15361 gtgtgacaga accacttgaa ttttcattta tgttcgttgc ttggccatta tatgtcttgc
    15421 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
    15481 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
    15541 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
    15601 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
    15661 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
    15721 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
    15781 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
    15841 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
    15901 tcgttgcaga tgagatggca aaactacatg gtggtgctgc agcacgacct gcacaaacaa
    15961 acaccgtggt taaaacagaa acatttgccg gtgaaacgat cgaacaagat atttacgcta
    16021 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
    16081 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
    16141 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
    16201 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
    16261 tatatgtaga agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
    16321 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
    16381 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
    16441 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
    16501 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
    16561 ccaactccgc ggtaataagt ccagctaaac gaaacatatg aagagctatt ttttatttga
    16621 ctcttcttat tacttgatgc agaacagctt tttcacaaaa tttttcttca tactcaaaac
    16681 taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt cttttaatgc
    16741 tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag aggaaatagc
    16801 taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg cttcttgagg
    16861 gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa taatttctaa
    16921 ctctttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag ttagattgcc
    16981 ggataattct aagatttctg gagagtcact ttctactagt acttttgctg cagcttctgc
    17041 atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa cgaattgacc
    17101 agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat tattccgtaa
    17161 aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt gatctggcgc
    17221 taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat aagatacccc
    17281 agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc ctggaacgcc
    17341 agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt ctaaatcatc
    17401 ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt tttcttcact
    17461 acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt ccagcgcata
    17521 accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca ctccctattc
    17581 tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat cataaagagt
    17641 gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag tattctctgc
    17701 aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat acccgatcac
    17761 tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca
    17821 tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg cagccttccc
    17881 ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc gaaggtaata
    17941 cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc
    18001 aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta
    18061 agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa aaaagaccag
    18121 aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg catttttctg
    18181 atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt gtcatgcccc
    18241 tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt
    18301 tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa gctatttccc
    18361 atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat attatcatgg
    18421 tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa ctcccatttc
    18481 gactacagca tttggacccc caacataggc gtaatcttct acttctctta aaacatgccc
    18541 aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta aagcgcttac
    18601 aacaagatac gttccttttg ggaatgggat gattcgcgta gtgtttggaa catcctgact
    18661 cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga ctgtaaagaa
    18721 ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga taactttttg
    18781 ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac tcttcaattc
    18841 cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact ttgccatttc
    18901 tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa atgacacgat
    18961 tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt tcactgcaat
    19021 ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag agatctcggg
    19081 agcttcgact aacccataat ctgaagaagg aagccattct gcatagattt tcccccaagt
    19141 atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag tgtgttcttc
    19201 tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa agccaatcat
    19261 gtgcatcaaa ctgccctttt cttccaagcg gtctccatca aatccataag aaacgttgat
    19321 tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag tctccgtgat
    19381 cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag gaactttttt
    19441 cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca tcgattttcc
    19501 tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc cgtatctact
    19561 gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt
    19621 ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagaa gatcgcgatt
    19681 agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg caataaacgt
    19741 aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta ttttatagga
    19801 aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat ctttaagcat
    19861 atttatcttc ctccttgatt gcctttttat catattatca aagaaagtca attacttgat
    19921 gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa gaaaattctc
    19981 aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt acctttagaa
    20041 aaataaattt tggggaagac agattctaag taaacaaagc ggtaaaaata tcttttattc
    20101 ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa actgctgatc
    20161 agaacgctgc ttggattttt ctttgcgata catcatacct cttttatcaa ttggaacaat
    20221 tatttatttc taacaaaatc acttcatttt tgtcagataa ataaaaaaca aaaaaagttc
    20281 catctttaaa acacgctatt cttttacttt ttctcacatt ctctaagaaa agaaaaggag
    20341 aatgaatttt tgacaattcg agcaaaagtg attatcaaat atgtaaaatc agctactttt
    20401 ttcttaaacg tttctttata ctaaatataa ataagtgttt catgaaagga gaactagatg
    20461 aataattggc ttgagtattt tcctgaaaac gttttggaac gcggttacag ctatcatctg
    20521 cacggttttg tccgtcattt gaactataca agcaagtact tgtccgcaac agtttctggc
    20581 acggaagatt ataaggtagt gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt
    20641 ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa
    20701 gaacgtccaa taaaaaaatc caactactca ctttccgaac tctcgtccct tgtttcctcc
    20761 gctagttcct ctcttgtacg ggagctttta acagagattc ttatagaaca tccgcacttt
    20821 atcgaacgct tcaaagttaa gatgccattt catgcaataa attattcaga taaattaaca
    20881 actatcatcc ataaatacga tcatattatc aagaaaaaca aaaacaggaa aacagcgaag
    20941 ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg aatcgcttat tcagcaaaat
    21001 gcctatcttc ccgcattcga attgatcaat gaagtgattg ctactctcga aacattctat
    21061 tgggagcctg aggatgaacg aacccttctt ctgatagagg actgttatta tttatggaaa
    21121 gaattattag cagaagcacc tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc
    21181 caagtcgatc atactgacgc ctcttattca aaacgctact ctataaaaat cctgaaagaa
    21241 gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa tagataaaaa actaaagaaa
    21301 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    21361 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    21421 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    21481 aagtcatgac aaatttcctt cgttgctgga tagattttcc ggcattgttg atagatttta
    21541 tgatactttt ctacattttg taagagaggt tccgctgcat ctgtatattg gataaaattc
    21601 tgacaacaac tttcaggtgt gtcgaaccac cctaacccta gtgcagcaat cattgttgcg
    21661 cctaatgcag gtccttcctc cacacttaaa caatagaccg ttgcatcgaa aatatcagct
    21721 tggatttgta gccatgcaag atttttcgcg cctcctccca ccgaaacaat acgtgtaaat
    21781 ctgcgacccg ctagttcttt acgtttcaat cccaccattg acaaatgctc attaaaaatt
    21841 tagaatattc acaagtttaa cgacaattat gaaaatttat gtcgtgctaa aggagttttg
    21901 ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttc
    21961 ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg atttcatcca
    22021 ctagtcgtca cgttgatgtc cctgctggtc tatgctggtt ctgcccaatt tatcacagtc
    22081 agcatgcttg ctagtcacag cccattgctt tccatcgttt tctcgacctt tctagtcaat
    22141 tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt
    22201 caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa
    22261 ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct
    22321 tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat
    22381 ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt
    22441 caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact
    22501 ttgattctgg tatatgtcgg actgattttt atcccaagca acttagtgat tgtagttgta
    22561 acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt
    22621 gctgactatt ttaggttgta ctttagcaac ttggctttct agggtcctac catttgtctt
    22681 actgaaaaaa ttcgatttac cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt
    22741 cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca
    22801 gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa
    22861 aagcttattg gtgatcgtct tggcaggaat tctttccctt tcgttgattc gcttattata
    22921 aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc
    22981 aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact
    23041 tttcctaaca tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc
    23101 atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca
    23161 gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg
    23221 aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc
    23281 tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg cttgatttga
    23341 tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat
    23401 tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt
    23461 ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc
    23521 gctagttgat tcaaaatcga accgacgccc ccggatccgt tgatcactaa gatttttttc
    23581 ccgcgattgg cattttcttc tggagttaga ccaaattttt caaataacaa ttcataagcg
    23641 gtcaatgagg tcaacggcaa ggctgcagct tcttcatctg aaagattttt tggtgcaaga
    23701 gcgacaatac gttcgtcaac tagttggtat tcttgattgc ttcctgctct tgttgttgta
    23761 cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcgtcacc tacagcaaca
    23821 actttcccga ctccatcaaa acctaagata cgtaaagcat ttcttatccc gttatcttgg
    23881 cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt
    23941 gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcggc
    24001 aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct
    24061 tagcttcatc aagttggagc atccatacag tgttcatttc aatatatata gtatgctatt
    24121 tgttccacct tttttattca tcataattat tctcttactt aaagtaaact atttgcactt
    24181 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctcttata aacggtgttc
    24241 tcctaccaac tcttcggtca caagtcaaaa tattcatcaa acatgagaag ctgttttttg
    24301 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctgat ataaacggtg
    24361 ttcaatcaac tgaccctcgg tattagctca aaaaacgaca aaatatgaag agctattttt
    24421 gtttggctgt tcttattact tgatacagga cagctttttc acgacctcta attcacggtg
    24481 ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtctcta
    24541 agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg
    24601 ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg
    24661 ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt
    24721 ctttttctgt atgttccctc gggtcttttt caaatgcata aggattcttg aaaatccctc
    24781 ggccaatcat gatgccatca acgccatatt tctcggccaa ttcttctcct gtctggcgat
    24841 cggggatatc tccattgatc gtgattagtg tttgtggagc gatttggtca cgtaaagcaa
    24901 tgattttagg aatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca
    24961 aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct
    25021 cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag
    25081 ctgcttcaat caattgtgcc gctacttctg gacgaaggat caagccactg ccttttccac
    25141 gttctgctac attagggact gggcagccca tgttgatatc gattccttgg aagcccattt
    25201 ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa
    25261 ccatcggctg ttcatcttct gtaaatgtta atcttcctcg tacactgtct tttccatctg
    25321 gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat
    25381 gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa
    25441 aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc tccattctat
    25501 caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa
    25561 gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaaaga ctgtgacaaa
    25621 aatcttatca cagtccattt tccggaataa atggtgttca aaagcttgca tttgcggtaa
    25681 taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg
    25741 atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg
    25801 tattcgctca aaaaacaaca aaatatgagg aactattttc ccgtttgttt ctctaatact
    25861 caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg
    25921 atcaaatacc aagtaacggc gtacaaaaca acgatgaata acacaagtac gatcatcgat
    25981 gttgctactg gcacatcgat cgttccaagg aatccgtaac ggaaaccgga gatcatataa
    26041 acgatcggat tgacttttga aacagcttgc caaaagggcg gtaacataga gatggcatag
    26101 aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatca
    26161 tcataagact gagcaaaaat cccgttgagc agtcctgcta atgaaaacaa gattgccgtc
    26221 atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag
    26281 gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccacaccacc aatcacaaag
    26341 ccccaaataa tcacatgcgt aggaacaggt gctaccaaga tttcttcgat atttttctga
    26401 aatttttgag agaagaagga cgaagaaaca ttggcatacg aactagtgat agcagacatc
    26461 ataatcaaac caggaacgat gaattccata tatgaaaaac cacccatgtc accgatccgc
    26521 ccaccgatca tttttccgaa aataacaaaa tacaaggatg tcgtgatcac tggcggaacc
    26581 agtgtttgga cccatatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat
    26641 gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt
    26701 caagaacagt tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg
    26761 tcttgtaagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat
    26821 tgtctgctca tcttcaaatg tacaagcata tccttcaatc acaggtttgg tatcatatgg
    26881 cgctaaatca aagataaatg tttcaaattg cagttttgac agaagctctt tcatacttgt
    26941 attctcgatc agttcacccg attggatgat tccgatgttg cgacaaagca tttcagcttc
    27001 ttctagataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcgtagaaa
    27061 ctcccacatc tcgcgtctca actcgatgtc aactcctgct gttggttcat ccaagatcag
    27121 caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga
    27181 aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc
    27241 gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat
    27301 ttgctggacc gtttcaaaag ggttaaaatt gaactcttgt ggaaccaatc caatctgttg
    27361 ctttgctcga actagatcct tgtccagatc ataaccgaag acattcactt ttccagatgt
    27421 tttattgact aaagaagtaa taatcccgat cgttgtagac ttcccagctc catttggccc
    27481 taatagcgca tagaaatctc cttctttcac cgaaagatcg attccccgca atgcttcgac
    27541 cccacttcca tagatctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct
    27601 atctccttat agacatgatt cttaattgta tcatggaata cagcagatga aaaaacaact
    27661 tggcttccac acaaaaacac acctgctaac aaagaggaga tgtgttttct agttcatgat
    27721 catcttcgat agaataacta tctaaacaac tctggaactt ttaaaatcat aaaagtaccg
    27781 acagcaacta ctacgatgac atatagaaga tccttataac tgaggttctt gtctttttta
    27841 ttcatgttaa gctttcctcc gtccttttat cgaaagcttt tttcttttat tttaaccgaa
    27901 ataaataaaa tggaaatagt tattttcaat ttctgttttc gctgtttgtt ttacggagaa
    27961 ctttgccatg gaacgtgcgt ctcgtcgctg atgctaagtt ttttcgataa aagctgctag
    28021 aaggaagaca gcgtggttta ttttgtatcg tcttttgcta aatcttccaa gatcatctgg
    28081 acagcagact cgttcccatt gaattctgcc acttttttgg catcaatgta cttcttttga
    28141 taatcgtctt tttggaccca cgcatcatgc ttcgtttgat aaagttaatc aaacggatac
    28201 cactgacgct gttcatcgaa caacaaactt tctttttggg cctgttcaac tatggattta
    28261 ggataattca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
    28321 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
    28381 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
    28441 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
    28501 tttgttcctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
    28561 tccagcaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
    28621 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
    28681 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
    28741 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gactggatta
    28801 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
    28861 aactctggtc ggcaagtgat cggcatatag gtgcgataat tcaaaacggc tgctgcaact
    28921 tccaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
    28981 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
    29041 atcaattctg cttcactccc gctcttcatt cgaaatgaaa aacctaattt agggatgtct
    29101 cttaatggtt tagtttgctg tttcagctct ttttgaagtg gtgtcgtcat ttttgctaac
    29161 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
    29221 gtttgtttct tcactagata atagaccgta ttgaatacta agccaccgat tgctagatag
    29281 attcctagta cttgccctaa aagaagcaaa cctacaccca ccagcggaag caagccaaga
    29341 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
    29401 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggattct ttctctcagt
    29461 tgaggatttt cctgataaaa agcaattagt ttttctaact gataatcttc tccaaaatca
    29521 aagctacgta aacgctgata caacgcttct gaacctacac tagaataggt actgttgatc
    29581 ccttcaaata cttcgaacat atccaagtca taccaagtaa gatcgtcgat ttcactatcc
    29641 catgaacgga attttttctc cgtgagccac gcatccttca aactttcttc tttatcaaat
    29701 ctcggttgat aaggaatttt tccccaatta ctacgaactg tttcctttac tttcaaacgt
    29761 gaaaaaatgt ctaaaacaac taataaaata acaactgcaa taataattaa aacgataaat
    29821 tctctttggc ccatatccct ctccgttcct tgatttgtta gttttagtat agcactgttg
    29881 cgctatttga aaagggggta caaaaataac tgtcttcgtt acctaattcg tatcaatcac
    29941 accaaaataa aaacaccttc caaaaaagaa gatgtcttta aaacgagata caattttttg
    30001 tcattcactt caggtaaatt agagccaatt aaaccctccg caacttgacg gagatggaga
    30061 agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg
    30121 acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa
    30181 aacgctggaa gactttttca acagaggttt gcgctcctgg tttgatagga ttggccactg
    30241 ttaatcgaat gaatgaatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac
    30301 gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata
    30361 cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt
    30421 cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca
    30481 tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta
    30541 atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta
    30601 cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag
    30661 aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt
    30721 gttcattttc atccacccat gtattcagtg catctactag gtccattaga ttcttttctc
    30781 gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct
    30841 tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa
    30901 cgagaagcaa cagccactta atcaaatcta aaccccactc cccacaccgt atgaatatac
    30961 acagcatgtg cctttgctag tttccctcga agattggaaa tatgcatatt cactgttttc
    31021 tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact
    31081 tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca
    31141 gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag
    31201 atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt
    31261 cgtaacaaca gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc
    31321 aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc
    31381 gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct
    31441 agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa
    31501 gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaagaatg aacagcttca
    31561 tcgtcttcga taatcaaaat agtttccagc aggaacacct acttaatgtc agatttttgg
    31621 aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt
    31681 ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc
    31741 gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaacgaag
    31801 gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg
    31861 aatgtcaaag ccactggaac agataatgtc gctaatatca gcagaagcaa tacaggaaat
    31921 acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca
    31981 agtgtgtaag caatcatggc tagccccgta atcaaaacca cgccaagtgt tgtgatcaaa
    32041 agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta
    32101 ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa
    32161 gccagaagaa tccatgcagc cattttttcc accattaaaa tccctgttgc accgttgatg
    32221 actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc
    32281 caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc
    32341 ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa
    32401 ttgtaagaaa taatcttcca gtttttgccc aactaggtgg ataccatcca caggaacttt
    32461 tgcctctacc aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat
    32521 aacacggcta gattggacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc
    32581 ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc
    32641 tttcgataat tctttgatca agtgtccttc atgcaaaata ccgtagtgag tggcgatctg
    32701 tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa
    32761 acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc
    32821 atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg
    32881 catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt
    32941 cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt
    33001 atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg
    33061 tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat
    33121 caaacccata atagttctca taaaggtaga cttacccgct ccattctccc caatcaaacc
    33181 atagatcatt ccacgtttga tttcgatcga tactttatct aatgctttgg cagcaccgta
    33241 ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct
    33301 tctttcttat tgataagtga agtatagttg acgggtataa agaaagcagc cagaaatgta
    33361 taaagaaagt gtaaattttc caattgaatt tcaagaaaaa tcagactgtc tcgcagacgt
    33421 ggttgagttg tctgccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc
    33481 accatggtaa tcgttgttat cgttgacatc agacgtcctt gtatatgcgt tggaacttct
    33541 cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat
    33601 gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa
    33661 aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca
    33721 caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg
    33781 aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt
    33841 tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt
    33901 aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta
    33961 ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac
    34021 tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga
    34081 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    34141 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    34201 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    34261 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    34321 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    34381 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    34441 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    34501 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    34561 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    34621 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    34681 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    34741 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    34801 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    34861 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    34921 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    34981 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    35041 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    35101 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    35161 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    35221 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    35281 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    35341 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    35401 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    35461 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    35521 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    35581 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    35641 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    35701 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    35761 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    35821 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    35881 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    35941 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    36001 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    36061 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    36121 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    36181 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    36241 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    36301 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    36361 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    36421 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    36481 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    36541 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    36601 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    36661 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    36721 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    36781 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    36841 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    36901 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    36961 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    37021 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    37081 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    37141 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    37201 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    37261 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    37321 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    37381 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    37441 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    37501 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    37561 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    37621 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    37681 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    37741 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    37801 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    37861 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    37921 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    37981 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    38041 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    38101 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    38161 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    38221 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    38281 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    38341 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    38401 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    38461 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    38521 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    38581 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    38641 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    38701 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    38761 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    38821 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    38881 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    38941 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    39001 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    39061 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    39121 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    39181 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    39241 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    39301 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    39361 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    39421 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    39481 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    39541 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    39601 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    39661 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    39721 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    39781 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    39841 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    39901 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    39961 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    40021 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    40081 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    40141 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    40201 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    40261 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    40321 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    40381 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    40441 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    40501 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    40561 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    40621 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    40681 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    40741 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    40801 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    40861 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    40921 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    40981 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    41041 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    41101 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    41161 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    41221 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    41281 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    41341 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    41401 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    41461 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    41521 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    41581 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    41641 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    41701 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    41761 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    41821 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    41881 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    41941 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    42001 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    42061 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    42121 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    42181 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    42241 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    42301 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    42361 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    42421 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    42481 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    42541 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    42601 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    42661 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    42721 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    42781 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    42841 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    42901 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    42961 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    43021 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    43081 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    43141 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    43201 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    43261 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    43321 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    43381 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    43441 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    43501 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    43561 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    43621 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    43681 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    43741 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    43801 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    43861 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    43921 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    43981 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    44041 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    44101 atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta ttgttattct
    44161 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    44221 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    44281 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    44341 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    44401 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    44461 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    44521 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    44581 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    44641 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    44701 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    44761 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    44821 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    44881 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    44941 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    45001 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    45061 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    45121 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    45181 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    45241 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    45301 gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac tgccactcgt
    45361 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    45421 tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt ctctacgctg
    45481 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    45541 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    45601 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    45661 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    45721 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    45781 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    45841 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    45901 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    45961 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    46021 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    46081 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    46141 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    46201 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    46261 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    46321 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    46381 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    46441 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    46501 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    46561 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    46621 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    46681 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    46741 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    46801 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    46861 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    46921 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    46981 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    47041 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    47101 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    47161 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    47221 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    47281 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    47341 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    47401 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    47461 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    47521 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    47581 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    47641 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    47701 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    47761 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    47821 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    47881 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    47941 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    48001 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    48061 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    48121 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    48181 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    48241 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    48301 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    48361 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    48421 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    48481 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    48541 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    48601 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    48661 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    48721 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    48781 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    48841 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    48901 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    48961 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    49021 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    49081 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    49141 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    49201 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    49261 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    49321 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    49381 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    49441 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    49501 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    49561 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    49621 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    49681 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    49741 ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    49801 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    49861 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    49921 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    49981 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    50041 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    50101 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    50161 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    50221 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    50281 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    50341 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    50401 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    50461 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    50521 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    50581 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    50641 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    50701 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    50761 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    50821 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    50881 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    50941 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    51001 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    51061 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    51121 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    51181 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    51241 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    51301 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    51361 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    51421 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    51481 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaataca
    51541 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    51601 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    51661 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    51721 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    51781 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    51841 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    51901 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    51961 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    52021 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    52081 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    52141 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    52201 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    52261 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    52321 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    52381 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    52441 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    52501 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    52561 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    52621 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac
    52681 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
    52741 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
    52801 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
    52861 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
    52921 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
    52981 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
    53041 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
    53101 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
    53161 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
    53221 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
    53281 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
    53341 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
    53401 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
    53461 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
    53521 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
    53581 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
    53641 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
    53701 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
    53761 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
    53821 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
    53881 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
    53941 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
    54001 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
    54061 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
    54121 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
    54181 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
    54241 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
    54301 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
    54361 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
    54421 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
    54481 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
    54541 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
    54601 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
    54661 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
    54721 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
    54781 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
    54841 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
    54901 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
    54961 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
    55021 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
    55081 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
    55141 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
    55201 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
    55261 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
    55321 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
    55381 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
    55441 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
    55501 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
    55561 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
    55621 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
    55681 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
    55741 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
    55801 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
    55861 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
    55921 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
    55981 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
    56041 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
    56101 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
    56161 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
    56221 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
    56281 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
    56341 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
    56401 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
    56461 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
    56521 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
    56581 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
    56641 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
    56701 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
    56761 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
    56821 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
    56881 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
    56941 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
    57001 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
    57061 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
    57121 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
    57181 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
    57241 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
    57301 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
    57361 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
    57421 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
    57481 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
    57541 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
    57601 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
    57661 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
    57721 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
    57781 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
    57841 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
    57901 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
    57961 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
    58021 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
    58081 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
    58141 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
    58201 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
    58261 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
    58321 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
    58381 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
    58441 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
    58501 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
    58561 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
    58621 ttgtatgtca attcgccaag agggtttgtt tgatccacga actgtgacaa ctgagaagaa
    58681 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
    58741 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
    58801 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
    58861 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
    58921 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
    58981 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
    59041 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
    59101 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
    59161 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
    59221 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
    59281 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
    59341 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
    59401 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
    59461 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
    59521 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
    59581 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
    59641 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
    59701 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
    59761 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
    59821 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
    59881 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
    59941 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
    60001 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
    60061 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
    60121 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
    60181 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
    60241 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
    60301 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
    60361 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
    60421 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
    60481 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
    60541 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
    60601 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
    60661 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
    60721 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
    60781 ttttccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
    60841 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
    60901 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
    60961 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
    61021 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
    61081 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
    61141 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
    61201 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
    61261 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
    61321 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
    61381 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
    61441 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
    61501 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
    61561 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
    61621 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
    61681 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
    61741 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
    61801 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
    61861 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
    61921 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
    61981 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
    62041 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
    62101 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
    62161 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
    62221 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
    62281 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
    62341 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
    62401 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
    62461 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
    62521 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
    62581 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
    62641 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    62701 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    62761 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    62821 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    62881 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    62941 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    63001 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    63061 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    63121 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    63181 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    63241 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    63301 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    63361 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    63421 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    63481 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    63541 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    63601 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    63661 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    63721 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    63781 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    63841 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    63901 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    63961 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    64021 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    64081 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    64141 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    64201 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    64261 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    64321 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    64381 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    64441 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    64501 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    64561 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    64621 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    64681 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    64741 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    64801 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    64861 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    64921 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    64981 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    65041 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    65101 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    65161 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    65221 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    65281 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    65341 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    65401 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    65461 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    65521 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    65581 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    65641 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    65701 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    65761 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    65821 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    65881 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    65941 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    66001 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    66061 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    66121 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    66181 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    66241 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    66301 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    66361 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    66421 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    66481 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    66541 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    66601 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    66661 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    66721 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    66781 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DACIWG010000008        65895 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.008, whole genome shotgun sequence.
ACCESSION   DACIWG010000008 DACIWG010000000
VERSION     DACIWG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.008"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(40..1464)
                     /locus_tag="IYF54_000506"
     CDS             complement(40..1464)
                     /locus_tag="IYF54_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830867.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            1675..2121
                     /gene="nrdI"
                     /locus_tag="IYF54_000507"
     CDS             1675..2121
                     /gene="nrdI"
                     /locus_tag="IYF54_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ9830868.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(2191..4173)
                     /locus_tag="IYF54_000508"
     CDS             complement(2191..4173)
                     /locus_tag="IYF54_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830869.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4187..5083)
                     /gene="murQ"
                     /locus_tag="IYF54_000509"
     CDS             complement(4187..5083)
                     /gene="murQ"
                     /locus_tag="IYF54_000509"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ9830870.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(5086..6147)
                     /locus_tag="IYF54_000510"
     CDS             complement(5086..6147)
                     /locus_tag="IYF54_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9830871.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(6359..6886)
                     /locus_tag="IYF54_000511"
     CDS             complement(6359..6886)
                     /locus_tag="IYF54_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9830872.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6953..8455)
                     /gene="lsa"
                     /locus_tag="IYF54_000512"
     CDS             complement(6953..8455)
                     /gene="lsa"
                     /locus_tag="IYF54_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9830873.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(8713..9834)
                     /locus_tag="IYF54_000513"
     CDS             complement(8713..9834)
                     /locus_tag="IYF54_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9830874.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9960..10901
                     /locus_tag="IYF54_000514"
     CDS             9960..10901
                     /locus_tag="IYF54_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ9830875.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(11044..11940)
                     /locus_tag="IYF54_000515"
     CDS             complement(11044..11940)
                     /locus_tag="IYF54_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ9830876.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            12222..13562
                     /locus_tag="IYF54_000516"
     CDS             12222..13562
                     /locus_tag="IYF54_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830877.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            13757..14200
                     /locus_tag="IYF54_000517"
     CDS             13757..14200
                     /locus_tag="IYF54_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ9830878.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(14462..15880)
                     /gene="nhaC"
                     /locus_tag="IYF54_000518"
     CDS             complement(14462..15880)
                     /gene="nhaC"
                     /locus_tag="IYF54_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ9830879.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(16284..17507)
                     /locus_tag="IYF54_000519"
     CDS             complement(16284..17507)
                     /locus_tag="IYF54_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ9830880.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(17539..18378)
                     /locus_tag="IYF54_000520"
     CDS             complement(17539..18378)
                     /locus_tag="IYF54_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ9830881.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(18395..19075)
                     /locus_tag="IYF54_000521"
     CDS             complement(18395..19075)
                     /locus_tag="IYF54_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9830882.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(19077..20150)
                     /locus_tag="IYF54_000522"
     CDS             complement(19077..20150)
                     /locus_tag="IYF54_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9830883.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(20800..21471)
                     /locus_tag="IYF54_000523"
     CDS             complement(20800..21471)
                     /locus_tag="IYF54_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9830884.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(21559..24237)
                     /gene="mgtA"
                     /locus_tag="IYF54_000524"
     CDS             complement(21559..24237)
                     /gene="mgtA"
                     /locus_tag="IYF54_000524"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ9830885.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            24897..25334
                     /gene="ndk"
                     /locus_tag="IYF54_000525"
     CDS             24897..25334
                     /gene="ndk"
                     /locus_tag="IYF54_000525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ9830886.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(25356..26222)
                     /locus_tag="IYF54_000526"
     CDS             complement(25356..26222)
                     /locus_tag="IYF54_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9830887.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(26456..28534)
                     /locus_tag="IYF54_000527"
     CDS             complement(26456..28534)
                     /locus_tag="IYF54_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9830888.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            29027..30244
                     /locus_tag="IYF54_000528"
     CDS             29027..30244
                     /locus_tag="IYF54_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9830889.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(30703..31326)
                     /locus_tag="IYF54_000529"
     CDS             complement(30703..31326)
                     /locus_tag="IYF54_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830890.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(31482..32879)
                     /locus_tag="IYF54_000530"
     CDS             complement(31482..32879)
                     /locus_tag="IYF54_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9830891.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(32901..33872)
                     /locus_tag="IYF54_000531"
     CDS             complement(32901..33872)
                     /locus_tag="IYF54_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9830892.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(33879..36179)
                     /locus_tag="IYF54_000532"
     CDS             complement(33879..36179)
                     /locus_tag="IYF54_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ9830893.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(36181..37506)
                     /locus_tag="IYF54_000533"
     CDS             complement(36181..37506)
                     /locus_tag="IYF54_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9830894.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            37737..38162
                     /locus_tag="IYF54_000534"
     CDS             37737..38162
                     /locus_tag="IYF54_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830895.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(38660..39361)
                     /locus_tag="IYF54_000535"
     CDS             complement(38660..39361)
                     /locus_tag="IYF54_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9830896.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(39377..39817)
                     /locus_tag="IYF54_000536"
     CDS             complement(39377..39817)
                     /locus_tag="IYF54_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ9830897.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(39817..40392)
                     /locus_tag="IYF54_000537"
     CDS             complement(39817..40392)
                     /locus_tag="IYF54_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ9830898.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(40803..41309)
                     /locus_tag="IYF54_000538"
     CDS             complement(40803..41309)
                     /locus_tag="IYF54_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ9830899.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(41320..41799)
                     /locus_tag="IYF54_000539"
                     /pseudo
     CDS             complement(41320..41799)
                     /locus_tag="IYF54_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(41971..42672)
                     /locus_tag="IYF54_000540"
     CDS             complement(41971..42672)
                     /locus_tag="IYF54_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9830900.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(42780..44222)
                     /locus_tag="IYF54_000541"
     CDS             complement(42780..44222)
                     /locus_tag="IYF54_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9830901.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(44236..46101)
                     /locus_tag="IYF54_000542"
     CDS             complement(44236..46101)
                     /locus_tag="IYF54_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830902.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(46222..47073)
                     /locus_tag="IYF54_000543"
     CDS             complement(46222..47073)
                     /locus_tag="IYF54_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9830903.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(47383..48096)
                     /locus_tag="IYF54_000544"
     CDS             complement(47383..48096)
                     /locus_tag="IYF54_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ9830904.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(48164..48490)
                     /locus_tag="IYF54_000545"
     CDS             complement(48164..48490)
                     /locus_tag="IYF54_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ9830905.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(48613..48840)
                     /locus_tag="IYF54_000546"
     CDS             complement(48613..48840)
                     /locus_tag="IYF54_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ9830906.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(49162..50415)
                     /locus_tag="IYF54_000547"
     CDS             complement(49162..50415)
                     /locus_tag="IYF54_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9830907.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            50476..51216
                     /locus_tag="IYF54_000548"
     CDS             50476..51216
                     /locus_tag="IYF54_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9830908.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(51302..52060)
                     /locus_tag="IYF54_000549"
     CDS             complement(51302..52060)
                     /locus_tag="IYF54_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9830909.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(52057..53085)
                     /locus_tag="IYF54_000550"
                     /pseudo
     CDS             complement(52057..53085)
                     /locus_tag="IYF54_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(53132..56272)
                     /locus_tag="IYF54_000551"
     CDS             complement(53132..56272)
                     /locus_tag="IYF54_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830910.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(56496..57380)
                     /locus_tag="IYF54_000552"
     CDS             complement(56496..57380)
                     /locus_tag="IYF54_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ9830911.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(57475..57912)
                     /locus_tag="IYF54_000553"
     CDS             complement(57475..57912)
                     /locus_tag="IYF54_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830912.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(58129..59040)
                     /locus_tag="IYF54_000554"
     CDS             complement(58129..59040)
                     /locus_tag="IYF54_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9830913.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(59497..59925)
                     /locus_tag="IYF54_000555"
     CDS             complement(59497..59925)
                     /locus_tag="IYF54_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830914.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(59953..60591)
                     /locus_tag="IYF54_000556"
     CDS             complement(59953..60591)
                     /locus_tag="IYF54_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9830915.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(60595..62631)
                     /locus_tag="IYF54_000557"
     CDS             complement(60595..62631)
                     /locus_tag="IYF54_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ9830916.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            62757..63422
                     /locus_tag="IYF54_000558"
     CDS             62757..63422
                     /locus_tag="IYF54_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9830917.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(64913..65785)
                     /locus_tag="IYF54_000559"
     CDS             complement(64913..65785)
                     /locus_tag="IYF54_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9830918.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT        19185 a        13911 c        11160 g        21639 t
ORIGIN      
        1 ttgaataaac cacaggcttc acagcctctc tttatacttc tatacgtgaa gcatcccagt
       61 ggcaaataaa accattgcga ttactccgac catcgtcaca ccgataagta aaatcttatc
      121 taagtggata tgttgtacag gttcgttttg ttctttacgt gctttgaggt aaaaataaat
      181 acctggtaag aaaagaacaa gaacaagcat cacttcatta aaggctacaa ctagcatggc
      241 ccatacttga aaagcacttg ctaaaaaccc tactaaaaat tggacccact ctttccgctg
      301 ataagaaagc ttcatttggt acagccctaa gaaaagccac gtgaagaaaa tcgctgctgt
      361 accaagtgta taagcaaagt tatatgcact gtcagtgaat aataatgaaa gtaaaaagac
      421 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
      481 tccccatgct ttaggcaaaa gtccgtcctc agccatattc cccattgttt caatcggcaa
      541 gattgtccat gagagccaaa ccccaactaa agaaatgatc aaaccgccgt tgatcacgac
      601 cgccccccat ttaccaacca tcatttcaaa tacatatccc atagcaggtt gcgctttgat
      661 cgtttgcagc tgattcatcg tcattgtgcc atatggaagc atggaaacca agacgtaaat
      721 aatgatcaaa ccgaatattc ctagaatcgt tgctttccca acatccgatt gtttttttgc
      781 tcgtgaacct acgacagaag ctccttcaat accaacgaat acccagagca tcaccatgat
      841 gcaactcaat acttgactag aaaacgggat cggctgccca ttcgcattca atatgatatc
      901 gctagaaaag ttattaatga attgttggat cgaaaaggtg ctgatagcta ctacaataaa
      961 taaaaataat ggcactaatt tacaaaatgt gacgatagca ttgacaaagc tagcattttc
     1021 aactccacga ttcactagcc atgcatatcc ccataagaag atcgtactca tcacgatcga
     1081 tgccacattt tgaccacctt cgaatacggg gaagaaataa cctaatgcac tcatcaacaa
     1141 tgttgcgaac gccacgtttc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     1201 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     1261 acgtttttta ttgatattat tgatcgataa ggagagcatc aacattgctg ttcctacgag
     1321 tacccagctg aagatagctg ggccaccagc cgatccggag atgtccgtca taataccaaa
     1381 tacgccacct ccaatcgctg aaccaatcac taaggcagca agtggaatca aaccaattcc
     1441 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa gtaaaaccgc ttttatcaaa
     1501 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     1561 tctaccgact tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     1621 ccaaaaaaac ttaaaaagat gctatactaa agggaaagaa ttgggggatg atccatgaac
     1681 attttatata tatcgatttc aggaaatacg cgtgcttttg ccaaacatct agcagaatat
     1741 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     1801 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     1861 gacggcggga atgggttaga caacggtgac acagaaatct tgaccgaaac gatgcgcgaa
     1921 tatctggaat acgaagacaa tcataaactt tgccttggtg tagtcggtag cggtaataaa
     1981 aacttcaaca atcaatattg tctgacagcc aaacaatacg cacaaagatt cggctttcct
     2041 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgcgt ttatcaaatt
     2101 ttggcagaaa aaaataaata acacaaaaga agaagctgaa aaataaattt cggcttcttc
     2161 tttttatgtt ctctattgtt tctattctta ttaagctttg acatccatca caggtgcttt
     2221 tgctttcgct ttacctgttt tttccaagac aaagtttttt acacgatcca tattggttac
     2281 caagacgatc atggaggttt ctttgtctgc tgctttgatt gcttctaagt cagcttctgc
     2341 tacaagcgta tcaggtgtca cttgctgtcc ttctgttact ttcacatcga atggcgtacc
     2401 gcctaagtca actgtgttga tgcccatgtg aagcaagatt tctaagccat ttggtgtcgt
     2461 aatgccgata gcatgttttg ttgggaaaac agaacttatt actccagtaa ccggggaata
     2521 gatatgtccg ttttctggtt ctacaaagta accttcaccc atcatttttt gtgcaaaaac
     2581 tgggtcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg ataagatagt
     2641 cacactttca gctgaagctt gaggtactgc tttttcaggt acttcattta ctgtggctgt
     2701 ttcggtcgct agttcttctt tttctaaacg gtctttagga ataccgaaga agaatgtagc
     2761 aacgaagcca ccagcgtaag cagccaataa accaaggata tagcctaacc atttaccatc
     2821 agagatcaat ggaatcaaag cggctcctga aggtccaata gcgattgctc cgacatttcc
     2881 gatcattccg atcactgccc caccgatacc tccaccgata caagcagtaa taaatggacg
     2941 tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctaaga ttccgactgg
     3001 taaagcccct ttgatcattt ctgttaattc tttgtctttt ttgcagcgga tccataatgc
     3061 caatgctgca cccacttgtc ctgctccggc catcgcaagg attggaagca ataaggtacg
     3121 accagtttca tcaatcattt gtacatggat tggcgtcaag atctgatgta gtccgaacat
     3181 gaccattggc aagaataatg tacctagaac gaagccggca aatgctcctc caacaccaag
     3241 tacccagttg atcccaccga ccatgccgtc agaaatgaac cctgctaaag gcatgatgaa
     3301 gaaaatttcg atcagaccga tggcaatcag ggaaagagtt ggcgtaacga tgatatctac
     3361 cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaataacc aaactgcgaa
     3421 gataacacct aagatcccgc cttgtccagt tgcaagagct tcaccggtaa acaagttttt
     3481 cagtggtgct tctggattca tccctgtcag catcacaaca gccccgataa ctccaccaag
     3541 cgttggcgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa ctagatagct
     3601 aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg ttcctgcgtc
     3661 tagtgttcca gctgtgacta agtttgctaa aactgcggca atccctgcca cgatcccagt
     3721 gccgacaaat gcagggatca ttggtacaaa gatattagag atgtctttta ataccgattt
     3781 gattttagag ggtttttgtt tcgatttttg tgccgctttc atttcttgtg cccgttcgtt
     3841 tacacggtct tttccagatg cagaagaact tgctgtagtt gcactagcag aaggaagttt
     3901 ttctcctaat ttcacgcctg ctaagtcaac catttcctgc gctactttgt tcactttccc
     3961 aggtccgatg atgacttgga gctgttcatc ctctactacg ccaagtactc ctggaatcgc
     4021 ttttaattcc gagacattta caagatcatc attgatgacc ttcatacgta cacgagtcat
     4081 gcagtggaca atgctttcaa cattgcccat accgcctgct ccttgataaa tttgttttgc
     4141 cagttcttcc aaagtcaatt cgttttttgc catggtcttt tccccctcat aaggttcctt
     4201 ttacaaaacc gtttgcttca gctaattttg cttgagctgc ctctttatca ctatttgtca
     4261 aaatcatgac gatagccagt ttgacatttt gatctgcttc ctcaaagtac ttttcagctg
     4321 tttcgtagtc agcttctgtc gcttgcatga tgattcgttt tgagcgttca attagttttt
     4381 catttgttgg tttgacgtcg accatcaagt tattgaatac tttaccgata ccgatcatac
     4441 tgatagtaga aatcatattc aaaatcagtt tttgtgctgt tcctgacttc aatcgagtag
     4501 agcctgtcaa aaattctggt cctgcatcga cttcgattgg gaaatccgca tgctcgctga
     4561 tttctgcttg tttattgcaa gaaatcgttc ctgtaacagc accaatcgat tttgcgtaat
     4621 ctaatccacc aatcacatat ggtgtacgac cgctagctgc gataccaata actatatcat
     4681 tttttgttaa attcagcttg attagatctt cttgccctaa ttcataagaa tcttcggctc
     4741 cttcgacggc tacagtcatt gcttcttctc caccagcaat taggcctatg accatttccg
     4801 gttctacacc gaatgtcggt acacattcag cagcatctaa tacaccaagt ctaccactcg
     4861 ttccagctcc catataaatc aagcgaccac cttttttaaa ggattcaatc gtgttggcga
     4921 tcactggctc gatacttggc agttcttttt caacagcagc ggccactttc tgatcttctt
     4981 tgttcatgag ttggactgct tgagaaatac tcatttcatc taatccaaaa gtttcttggt
     5041 ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc ttccttcttt
     5101 tcttataaat tgatagttca taccagcttt gatttggttg atcagcggta gttcttcttt
     5161 gatgactttg gctactacat tcactttttc atccgctggc aaatcatatt tagttaattg
     5221 gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagatc ctttcatacg
     5281 cttagctgta tttcttactg ggattggcgg aatctctcga aaacgcgcat ctgcgcttcg
     5341 tatgacatct cttgcatcat cttgccgatt cgtatgtttt cctaatatat attcgtaaaa
     5401 aacttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga aagaagcaaa
     5461 ttgctcctga acgtgttctg acagacctgc atctccgata taaacaatat ccgtgttact
     5521 tgctaataga tcaagagcac ctgccagagg atggatccct cgatgttctt ccaaagttgg
     5581 taatccttga tacagtggac ctctcaactc tgtatctcct ggtacaaagc cttgtacaag
     5641 gaatccttga gttttgagcc attgattttt ttcctgatac cattctttgt ccaaccctgt
     5701 ttcaagacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag ccgctgcttc
     5761 tttcaactca tcgatatcat taggcgtgat cgtgctggca ttcaaactga ttttcaattg
     5821 atgtgaccag tcggcgatct gctgattaga aatatgataa tccattctta accctgttac
     5881 accaattgct ttcaagggtg ttagatcttc taatgaaaag ccagctcgtt tcaatgcctc
     5941 tcctgaaata tcgaccatca actctagttt attctttttc gcttcgcttc ctagttcgat
     6001 caatcgattt tgataagccg aaacatcatc ttccggtata tgcatggaag tgaaaatacc
     6061 gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg acaaagaatc
     6121 attcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc gtcttcattt
     6181 ctatgactag agtatatatc taaagaaaca aaatttcaat acttttgtta aaataattaa
     6241 attttatttc taaaaattgg atttataatg ccaaaagaat tgacacagcc acaaaaaagg
     6301 cactgctaag caaaagcagt gccttgaaac attttattta aataacacaa aagtctaatt
     6361 actcataaga aattaattct gtattgtcta ccatgaatgt aaagaaccct tcattaggca
     6421 ttggatgaaa ataagcattg atcactctta tcaaggcatt atgacagact aacaagtaga
     6481 cattttcttc gtcttccatg cattctttca ataaaggaac gatccgtgcg tacacatcta
     6541 agaccgactc tccatttggg aaacgtacag caaacgctaa acgcgctttt tgaaagtttt
     6601 catctttgct tggcagtccg tcataatccc caaaatccat ttctaccaat cgctcatcag
     6661 cggtcatcgg caaattgaga cgatcaacta ctgcttgtgc tgtatctctt gctcgttgaa
     6721 gaggggaatg gatgattttc gtgattggaa caggcaaatc tactaccttt tctgctagtg
     6781 cctgtgcctg catctgtcct ttttcagtca gcggcacatc tgctcgcccg caaactaatc
     6841 cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata aagcatgtga ttctctcctt
     6901 cgttgacaaa gttctcctta accatacctt gttgtctttg agattacaag gtttagattt
     6961 tctcaagaga aataatctca gtagatactt tatctagaaa ggtttgatca tgttccacta
     7021 gtaacatagc aggtttcacg ttcaaaatca gttgttctaa ttgttcttga ttgaagacat
     7081 ccaaataatt caatggttca tcccatatat agagctcagc tggttgggat aaagatttag
     7141 ccaattccac ttttttccgt tgtcccatac tcatctgttc gattttatta tgaaaaacat
     7201 ctctttccat cccaagcttt cggagattat tcaaaaatgc ttgatagtct actcggtttt
     7261 tcttcgcgaa ttccgccaat gtccctcgat tgtcttcata attttggcgt acgtaactaa
     7321 tggtcaaatg tttgggctgg gatttttctc ctacaacctt gccgttgaat gtccccagaa
     7381 gataatggat aatggatgac tttcccgcgc cgttaggacc tgaaattgcc atccgctgat
     7441 gaggttcgat cgtaaaatga actggctcga ataacagatt ttcataccct aattgaagat
     7501 tttctacgct taacagccgt ttatgatgag acacttggct attcatcgtc aaagcatcga
     7561 tatactcgat atcttttaat aatttttctt tctcactgat ctgggtttcc atccgattca
     7621 cgattgcttt agatcgtttc atcgtccgtg cagcatcagc gcctactgct cctttattca
     7681 ctcgcctaga ttccgtatcg atgaatccga cttttttctt tgttttgtcc ccttctcggg
     7741 aacgagacca ttctgctttt tcagctgctg ttttctttag cctgctgact tcttttttca
     7801 atttttcatt tttagctatt tcaaattcat cacgaagttt tttctgttct tcatagatag
     7861 agaaattccc ttgataaagt tccagttggc tcttttcgat tgctaaaaca tggtccacta
     7921 cttcatcgat aaatccccga tcatgactca ccacgatgaa tccttgtttt ttctttttca
     7981 agtaagccgc tacttgtttt ctgccagaga tatccaaatg attcgttggt tcatcgatta
     8041 acgggaaatg agtgtcatcc acaaataaga gtgccagtag gacttttgtc ttctctcctc
     8101 ctgatagtgt gctgaattct ctccataaga tttcaggatc tgtttgcatc agttggagct
     8161 ctctttcaat ttcccatatc tcaaaatcca taatctcatt taacacataa taggtcaaac
     8221 gctctttatc ttttgtttgc tgcgggaaat agacaaacgc ttgctgatgg atcacttgcc
     8281 cttgataagg gagtttgttt tgcaggatat tcagtaaagt tgtctttcct cgaccgtttc
     8341 gtccgataag tcctagtttc cattgtgtgt caaagtttag attggcttgt tcaaataata
     8401 atgtgccttg gctgtcatag ccgaatgtca gattttttat ttcgatttta gacattattc
     8461 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
     8521 ttggggcaca caaaaagacc catacatcga aagaaccaag ccaatcccga aaatctgcac
     8581 actgctccct tgtctccaca aggtacagtc tgaatgcagc tttcatcaat cgacttaatt
     8641 tttcaatgca cggatccctc actttgtcct atatttgaaa ttacgatagc ataaagaaat
     8701 tgtttttgtc aactacttcg ggagaatgtt gaaaaatctg ctgagtgtct gtcctaagat
     8761 ttctgttttt tctaatgttt gcctgtgttc cattgcttcc tctaaatcga ttggtcctaa
     8821 ttgaatgctt aagacaatgg ggagaaaatc aaagagtcct tccagctctc ggtctctact
     8881 tccgcaaata gcgattattg gttttcgata tttttttgca agcactgcta cgccatatgg
     8941 aacttttccg tggattgttt gctgatcaat ccgcccttct cctgtaatga tgacatccgc
     9001 ttgttcgatc tgtttttcta atccagtgat ttggctgacc aactcaaaac cagagaccat
     9061 cttcgctcct aacaaaaaaa gggctccgcc aataccgcct gctgctccgc ttcctgggat
     9121 gtcatctaaa tccacctccc aaacatcttt tatctgctga gcaatctttt cagcttgttt
     9181 atccaagaaa gctaacgtcg tatccgttcc tcctttttgt cttccaaatt gccaagcagc
     9241 tccttgttga cctgtatatg ggttggtcac gtctgcagcg atgatcactt ttactttaga
     9301 aacaagaacg ttccttcgat ccaaatgaag ttttctagta gataacaaag gatttccttc
     9361 tgtgtaccct tctaaagaag cacccaaaga ttggagtaat cctagaccac catcgctaca
     9421 tccgcttcct cctaaagtga caatgatttg ttccacttct tgaagcagtg tatctttgat
     9481 aagctctccc aaggcataac tagaagcaag tcggatggtt tgatccgttg gtttatcaag
     9541 taaatctaat cctaatactt gtgcagattc aatgactgcg gtattttttc cttgccagtt
     9601 cgtcaataaa taactgattt ttcgctgacg aaataagaga tccatcccat caatcgtacg
     9661 ccaggttccc ccgagcgcgt aatagattgc ttctgcagta ttttctccgc catctgcaat
     9721 cggcacattg atttttcttc ctttacggtc ttcccaacca tttagaactg cctgatttaa
     9781 ttcaaatgaa gaagcacttc ctttaaatga atcgattgcc gccaaaatat ccatctatat
     9841 ccctcacttt cttcttaagt aagacacttt ttttcacttt tttcaaacaa aacaatgcta
     9901 ttttcttctg tttttctata tactaaaagg gaactcattg aataaaggag agaaaaaaca
     9961 tgaaaatcgc aatagctgga gccggagcaa tgggcagccg aatcggatta atgttgcatc
    10021 aaagcggaaa tgaggtcgtc ttgatcgatc gctggcctgc tcacattgaa gccattcgta
    10081 caaatggatt gattgctgac tttaatggca aagaagtagt tgcaaaactg ccaatctatt
    10141 caccagaaga aatcatcgaa agcaacaaac acgtggattt gatcgtagcc ttgacaaaag
    10201 cgaatcaatt agatgatatg tttcgttcga tccaatccat tattactgag aatacttatg
    10261 ttttgtgttt actgaatgga ttaggtcatg aagatgtact ggaaaaatat gtaccaaaga
    10321 aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc ggaaaagtca
    10381 cattattagg tgatggagaa atcgaactag agaacttgga accagaaggc gaagcattta
    10441 caaaaaaagt tgtagaagtc tttcaagaag caaaattgaa tccaatctat agtcataatg
    10501 ttcgttattc catttggcga aaagcttgtg taaacggaac attaaatgga ttatgcacga
    10561 tattggattg taatattgct gaattaggtg cgcaaaaagc ggcagagggc atggttcgaa
    10621 cgattgtatc tgagtttgct tctatcgcag caaaagaagg aatcacatta gatcaagaag
    10681 aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg cattacccat
    10741 ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac atcaatggtg
    10801 ccgtttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc tttttgaccc
    10861 aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc tgatgagaat
    10921 cagctggatc agcagctggt tctcattttt ttacccccat gaaagttcaa aaagaaaaac
    10981 acaattctca tgttgccata agaattgcgc ttgtttattt tcatctatga ttggttggca
    11041 cttttattta tttttaacag aggaaagatc gtattcatat tgttcataaa tagcggagtc
    11101 agtagtgaaa aaacgttttg cccaattttc tgcatcaaaa aattccaaca gttctgatgg
    11161 gattggttga aatggcgtat gcaaaaattc cggtaattca tctaaatcac gttcttgaag
    11221 caaaaaaata ttttcagaat gataaaactg ataatgagct atggcttgat tatctgtcac
    11281 tagtttcttt tgaaaataca tagattcaaa cgttcgcaaa gtcactcctt gctgcccttc
    11341 ccgcaacagt tctagaatcc ccctcgattc cccggttttt ttcagatatt cttcataagt
    11401 aaggtatcca ggttgttcat ttcctcgaag cggaagaata aagtaattgg tcgtaacacc
    11461 tctttttgta aattcctgct tatatttttc tgcccgttct tttctgccgt tatttgttgc
    11521 accaaaaaat agatccgtct ttggttcgct agaagaaaaa tccatatgtt tcgaataaaa
    11581 ggaactatta tgttttagtc caagttcttt cgcttctaaa aaatcaaaat gataaatctc
    11641 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttaaagtg
    11701 gttccaaaaa aataacacga tttttgcatt ggtaaatgga cgcacgcgtc gagccatctc
    11761 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtcgagc
    11821 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    11881 ggaaggaaaa aaataaagtg atttattttt cgcaattagc aatacgtcgt ttttgttcat
    11941 ctgttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    12001 gccagtaaaa cgcttcctat tatttttata aaaatttaac atttctatga aaatacgatg
    12061 aaataaatca ttcttccatt aaaatatgcc caaaagtttt ctgaatagtt gtaattatct
    12121 gaaaatttgt tatcattagt cattattatt attactattt attcttacta tttattctta
    12181 ctatgcattt tgattgtgaa tttaaaagaa agaggttatt tatggaagat aaaaaattga
    12241 aaaaacaact tagctcccga catatcacca tgctagcttt aggtggagcg attggtgcag
    12301 gtctatttaa aggaagtggc gaagcgattg gaatcgccgg cccttctgta ttgatcgctt
    12361 ttttgattgg cggcataatt ttatatatcg tgatgaaagg tctgggaaaa attgttttaa
    12421 gtggtggtga tacgcaccac ggattatctg gattgattcg tccatatcta ggtgctcatg
    12481 ctgccgattt taccgattgg gtctactggt ccatgtggat tatcaatatc attgctgaag
    12541 ctgttgcagc tgcttccttt ttacagttgt ggttccccca catcccagct tgggtatttg
    12601 tttttatgtt ggctgtttta actactatca tcaatcttta ttcggtacga ttattcgcag
    12661 agacagaata ttggctggcc tttgctaaaa tcagtgtcat tatcctattg atcatttttg
    12721 ctgcttatct tgtcggtcag caaatgttgg gtactggtgt tttccctacc cttcaacaat
    12781 taactgatca tggtggtttt actccccacg gtatgaaggg aatcatcagt tctttgcttg
    12841 tagttatcta ttcttacggc ggttctgagt tgattgctat cacagtaagc gaagcagatg
    12901 atcctaaacg tgcgattcct aaagcaatca aaggcgtcat gggaagaatc atttcctttt
    12961 acattattcc tttatttttg cttttgatta tttttccttg gaatacttta gcaggcacga
    13021 acgttagtcc ttttgtgatg gtatttgaaa aaatgaatat tccatttgcg gcagatatcg
    13081 tcaatttcgt gattgttctt gctctgttct cgtctatcaa ttcaggtgta tatgcttctt
    13141 cgagaatttt gttcttccgc ttgaaagatc ggaaaggttc ctctagaaaa ctagctgttt
    13201 taaacaagca tcaagtaccg caacgtgctg tgctgttttg taccagtgtt ctttatctag
    13261 gtgtggctct ttcttatttt gtcggtgaca aattatttgg ctatctggca ggttctcttt
    13321 cctatacagt attattgatc tggatcatta tcagtgctgc gtcctttgta ctgacactaa
    13381 ggaaaggaac gttatgggac aaaggaatca gcttttttgc attagcagtc ttatggttga
    13441 ttttccttgg tatcctattc acgaactcca ttggggtaac tgtattgacc ggtttgcttt
    13501 acctgttcat ttatttcagt tatcaaaaaa agaatgatgc tttcgtcttg acgaatgaat
    13561 gaaaccaata gagaatttta ttctatttat attgaatatc tggagctatt gaaaaaaagg
    13621 agctgcgaca aaaattgttg cagttctttt ttccgaataa acggtgttca aaaaccatct
    13681 gtgcagatta attgattgtt ctccacgctc atgacatgat agaagaaagg aatgaagaaa
    13741 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgattg gtgtttcgac
    13801 ggtagtcatg tttggtatga tgtatttgaa tgtttatgcg cttgatcata tcttcttcag
    13861 tcgcacacgt gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgttgtt
    13921 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgct atgattttag ccgctagtct
    13981 tcttgttttt gccggttcct tatttatggt acgaagccaa acaactgtca acgatacggc
    14041 gtggatgaag gcgatgatcc ctcatcactc tattgctatc ttgacaagta aacgagctga
    14101 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    14161 aatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatcca tccaaataaa
    14221 aaaagaggat gttccagaag tgatcaactt caagaaataa gaagaagcta cttctggaac
    14281 accgtgaatc agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    14341 aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcaaatgc aagcttttga
    14401 acaccgttta ttcgtaaaag ggagtgtgac aaaagtcttg tcacactccc tttttacgtt
    14461 tttattgttc taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttctttg
    14521 agatccccca acctgtaaag gcactgacca tcgaaaacac aggtgaaagc aaactgaaaa
    14581 acacgaatgg caagtaagtg agtgtaggaa tgccaaatgt acttgctacg aaacttccgg
    14641 cgattcccca aggcactaag tagttgatta ctgttccacc atcttctagt gttctcccta
    14701 atacagttgg atcaaatcct gctttttcgt aagtttcttt aaatgcatta ccaggcagga
    14761 taacagatag aaattgttct ccaataaaga tatttactcc gatgcttgtc atcaatgttg
    14821 taaaaatcaa ttttggggtg ttcgtcatct ttcttgatac tcgttccata acggttgaga
    14881 tcaacccaaa ttcgatcaac aaaccaccta atgacaatgt tagaataatc aatgacacag
    14941 ttggcatcat gctttcgatt cctccacggc taagtagtga atcgatttgt tggtttccag
    15001 tatttgaaac aaatcccgat tcgatgaccc ctgccaactt cgttaaggaa gtttgtgggt
    15061 tttgaataaa gatcattcct actgctacag caatattcaa caaaatcgtc aaaattgccg
    15121 gtaccttttt ccatgcacac aagagcatta aaaatagagg aatcagtgac cagatcgaaa
    15181 tagtaaagtt tgcttctaaa atatcagtca cttcacgaat agatgaaaga cttgcggctt
    15241 tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc ggaacagttg
    15301 accacatcat atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctaaat
    15361 ttgttgattc ggataatgga gacatcttgt ctccaaaaac ggcacctgaa acaatggctc
    15421 caacaacaag ggctggattt agtcctagtg ttgtcccaat accgaaaaat gcaatcccaa
    15481 tcgttgacat aacagtaaag gcactcccta cagcgctacc tacgattgca caaacaacaa
    15541 atacagaagg gacgaaccac tggacactaa tcaatttaaa tccaatgacc atgagggttg
    15601 gaataattcc tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga
    15661 ttggaatgat ccccggtttg atgccattga tgatcccttc gtgcagccaa tctgtcgggt
    15721 atttttttat cactgcgtac aacatgacca ttccgatagc tgccaaaatc gtgatattag
    15781 gtgaaacctt taatccgaca acgccaatcg caatgcctaa aacaatgacc atcaatacaa
    15841 gtgttgcttc taaaaaactt ggtgttcctt ttttttccat gataatctct ccttttttag
    15901 ttgttcttat gttctaaaaa agttaagata tcggattact ccgccacaat tcatccgcga
    15961 ttcctccttt ataaaaataa aaagacctgc tttcaactaa ggacgaatga ctccgcggta
    16021 ccaccttaat taaaaacaga tctgtttttc actttcaaac gattattcaa atgtatggac
    16081 tttccatgta attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac
    16141 aaaacaccta accactactg cgtgaaaagt tgacttgtat gaagtttacc ttccataaga
    16201 tcatttgtca atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt
    16261 tcgcgcacaa ctttatcttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    16321 atttcttgat gttcttgttt gattttttct aatagcttgg gttctctcaa taaatctaga
    16381 gctgtattgg caagtaattc cgctgcaatc gcaatagaag caagtccttt ttcactttta
    16441 gcagcagctt tgaattcttc tgtatgtccg gcaatatatg tctcagagat tgctacagta
    16501 ggctgtatcg ttggaataac ttggctaaca tttcctacat cagaagaacc tagacttgtt
    16561 ctttgttccg tttctatttc ttcatcagga acccctgctt cttttgcatg ttggaagaat
    16621 aagtcatcga aagaaggcgt tacgatgaca tcatcgactg cattttggaa caatccaaac
    16681 tcaaaagtcg tacccgtgct taatgcagca cctcgtacga tattttccac tttttcatat
    16741 actttgtcta atgtttgtcg atttcctgca cgtaaataga aacgtccagc agcgtagtct
    16801 ggtatgacat tggccgccac gcctccatct gtaatgattc catggatatt gacatctttt
    16861 ggcaattgca agcgtagagc gttgattccg ttaaacactt ggatcaaggc ttccaaagca
    16921 tttactcctt tttcaggtgc tgctgctgca tgagaagcga caccgtaaaa tttgatatcg
    16981 actggatcat ttgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacacat
    17041 aatgctgcat cgacatcctc aaaaaaacct tcccgaacga agcttccctt agctgatcca
    17101 ttttcgcctc cttcttcccc cggagtacca tacacacgaa tttctccgcc aatctcatca
    17161 atgaattgtt tcactatgct ggctgctaat acggagtaag taccaaataa attatgtccg
    17221 catgcatgac caatacctgg taaagcatca aattctgcca aaaaggcaat tgtcggacct
    17281 ggtttattgg aaatataacg tgcatcaaag cctgttcggt gcccagctac ttctttttct
    17341 acccagaacc cttctttttc caattgtttg attaaagctg ctgaggaata aaattcataa
    17401 ttacttactt caggatggtc gtggatagct agtgcaagtg cttgatattc tggtaaacgg
    17461 tattgaatca gatccttcaa ttgatttgtt gagagtgtgg ttgtcataaa aatttcctcc
    17521 tatgctagtc gttttttctt attttctccc gaatgtttcc cacgcaggta cgcttgaacc
    17581 ttttgatgtt ttttcgatta ctttggctgt ttcttctgtt tgataggcat ctactatttt
    17641 ttgatacgtt ttattgtctt catcttcttt tcttgcaacg atgatattga cataaggacg
    17701 tgcatgctca tcgactggtt ctaagaagat tgcatcagaa gcgggattaa atccagcatc
    17761 tacagctaca ccgctattga tgactgaaat atctacatct gacaaagcac gtgctgtttg
    17821 ggaagcatct aactcagaaa tgtctagatt ttttttattc tcggtaatat cagaaacggt
    17881 aggcgcttgg ccttttgcct tgtccacttt gatcaatcca gcagtttgca ataacagaag
    17941 agctctgccg ccattcgtga catcattagg gattgcaact tttgctccat ctttgatatc
    18001 agccacatct tttaacgtat tggaataaat tcccaacggt gcattaaccg tattcccaat
    18061 cgatactaaa tcagttccgt gttcttcatt gtaattgtct aaaaagaatt gatgttggaa
    18121 ggaattcaaa tcgatcgatc catctgctaa agccgcattt ggctgattgt aatctgtaaa
    18181 tttcacatat tccaaatgga tgttttcctt tttcaaacgg tctttcacat cgtcccatac
    18241 atctgtatct tccccaatga tccctagttt tacggtcgtt tctttttttt cagttgtagc
    18301 tgcttttcct tggctgcatc ctgccaacca aataattgct acaaatgtaa tgactgcaaa
    18361 acgaataatt tttttcattt tttctcctcc attttcaatg tgtgactttt ttaatcagtt
    18421 gattactgat aaattgactc aaaaaaacta agaccagaat caataatgta gcgacaatcg
    18481 ttacatctgt ttggaatcga ttatatcctc tcgatatagc aagattccct aatcctccgg
    18541 cacctactgc tccagccatg gtagtcagtc cgatcacatt gatgatcgtc aacgctgaga
    18601 cccgaatgat accaggtaac ccttctaaca aataaacacg taaaatgatg ccgattgggc
    18661 tcgtgcccat cgcttctgct gcttcgatca ccccaggatc aacttctagc agtgcattct
    18721 cgatttgacg ggcaaaaaac gggacaacgc caaaaatcaa agggactaaa gctgctttgg
    18781 caccaatcgt cgttccaact aagaaacgag tagtcaaagc taaaagagaa agcaagataa
    18841 tgaaagggat ggaacgaatg atattgatca acttatcaag tatttcgaac agcagcctgt
    18901 tagctagtaa tccatttgga cgtgtcacta ctaacgtcac acctaagatg attccgatga
    18961 tcccaccaat gaatgccgtc caaaaagaca tatatagtgt ttgctttgtc gcttcgatga
    19021 attcatccgg aatcaaatag acattcggca aatatgtttt aaaaaattca gtcattttag
    19081 tttcctcctt cgatgacttt gatacggtta tctaattcat ttttttgtaa aatagttact
    19141 tttacaccag atttaatcaa ataatccaat gctttttgcc gctgtttggt ttctccagct
    19201 aaagtaacga tcaaacttcc taagggtgtt tcttgtaata gctctacatt accgtataag
    19261 atattcgctg ttacttggta cttgctgtaa agatgagcga ttagaggttc gttcgtctgg
    19321 ttgccgatat aacttaattc caccaaccat ttatttgcaa tctgatcggc aaaagcagca
    19381 tgggcactga ttgtttccaa tgcttggtca aggtgtgtcg ctgtccgaat aaaatcttta
    19441 gtcaattctt cttcaggatg actgaaaatc tggatgctgc tacctttttc aatgattttc
    19501 cccgcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    19561 atcaggacga ttgttagacc tagctgacga tttagttttt ttaacagtgc aaggatctga
    19621 agggtcgttt tcggatcaag tgcactagtt gcttcatcgc ataacaacac tttaggttca
    19681 tttgccagag ctcgtgcgat tgccactcgt tgtttttgcc cccccgacag ttgtttagga
    19741 tacgcagatg ctttttcttc cagaccgaca agttccagta gttcattcac tttttgacga
    19801 cgttcctgtt ttgattttcc agaatacttt agagaaaaat ctacgttgtc aaaaatcgtt
    19861 cgactttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    19921 aattcttttg gagagagctg gcttaaattc gtctgattga cgatgacttc tcctgctgtt
    19981 ggtttctgca acaaattcat cacgcggact aacgtactct ttcctgcacc tgagtaaccg
    20041 acgatcccat aaacatcccc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    20101 tctttttctt tttgtttgaa acatacagaa atatctcgca attcaatcat taacattcct
    20161 ccttgtctat aaaaaaagca tcctcaagaa aatgaaaata gtcattttct tgaggacgcc
    20221 tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac
    20281 catcatactc tagcaagata tcggtcgcag ccgtcaccgc cttacatcta tgctcgggat
    20341 gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct
    20401 ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa
    20461 aatcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgtgg taccaccttt
    20521 tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt
    20581 aacggtcgaa catttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt
    20641 cagcttttct tacttgactc ttcacaccag ccagagcctc tctgcaaagt ttccaagctt
    20701 actttccttc tcaacgaaat caatattttg ttgttgggaa taatctacag gaggaaaact
    20761 gaaaagtcaa gcatgaaatc caaattattt tttctatatt tatttttctg tttttttatc
    20821 taacatttca ataacagttc gaagatcttt tccttccgca agaaggttca atttttcctt
    20881 ctcttcgacg ctataatttc gaacaaaact ggaatcggga atcaaaacaa cgcctagtcc
    20941 tgcacgtgaa gcggctgtcg ctcctactaa agaatcttcc actgcaaaag ctgaaagagc
    21001 ttctaatcct gttcgttcca aagctgtcag ataagggact ggtgaaggct tgttctccga
    21061 tacttcatct ccgaacgtaa acgtatcaaa gtaatcggct aattgaaagt gatccaagat
    21121 atctgttgct cttttcgtaa cagttgtcgt agcaatccct agtttcatat gacgttcttt
    21181 taagtctgtc aatatttctt ttgcataggg tttcaattgg atagctccac ttgtcagctg
    21241 ttgataaaaa tattcttcac gctttgctcg gacttcctgc actttttcag gactgcctgc
    21301 tgcctgcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat
    21361 ttctttcggg atatgaatat caaattgctg gaatccccaa agccaaccat cccgatacgt
    21421 tctttccgta tccactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt
    21481 aatcctttcc aaaacaatac acctagttta gcatgactga tccaaaatga acagaagcat
    21541 agagcccagc cgcatcactt ataagagttc ttggaatcga cggatataga ttttcttcac
    21601 agctgtcaca agcgccagat aagcgacgac tgttataaaa aaccataacc agtaattgcc
    21661 agggagcggc atcaagccta actcagcacc aaatcctgtg aatggaagga ctgaaccgac
    21721 aatgattcct aatgtagtga taaccagcat ggcaaatgtt gcattgcttt ggataaatgg
    21781 tattttcggt gtacgcaatg catggagtac taatgtttgt gtccacaggg attcgacaaa
    21841 ccatcctgca tggaataatg cgataaaaat cacttgttgt gagctatcca atgtatggaa
    21901 acttccacca actacagcag gacaaataac aaaatacatc aacaagtaag tagtgatatc
    21961 aaaaatcgaa cttgttggac caaaataaac catgaatttt ccaatactag atgcattcca
    22021 tttctttggc tcatccaaat attctttatc catccgatcc catggtaagg aaatgcaaga
    22081 aacgtcatag atcagattca aaaatagaag ttgcaaaggc aacattggta aaaatggtaa
    22141 gaacgtactg gcaatcagta cggaaaacat attgccaaaa ttagaacttg ctgttgcctt
    22201 gatatatttc atgatgtttc caaaagtttc tcttccagaa agaaggcctc tttccaaaat
    22261 cgtcaaatct ttttctaaaa ggatcacgtc tgctgattct tttgcgatat ccacagctgt
    22321 atccacggaa ataccgatat cagccacttt cattgctgga gcatcattga taccatctcc
    22381 taaaaatccg acagtatgcc catttttacg taaagctgtc gtcaatcgtg ctttttgctg
    22441 aggattcaat tttacgaata cttcgtgttc ttcagctact tgcgctaatt catgatcatt
    22501 catttccgag attttctcac cagtaatcaa ttcattgatt tctaggccta cttgacggca
    22561 cacagaccgt gtgactaatt cattgtcccc agtcagaact tttacagcta caccatgttc
    22621 ttttaaagct ttcaacgctt gttttgttgt ttcttttggt ggatcaagga aagcaagata
    22681 accaatcaga accatgtctg actcatcttt gactgaaaat tctccaacaa cgcttggatt
    22741 ggtcttttgt gcgacaccaa tcactcgtaa gccgtcttca ttaagtgatt gtacttttgc
    22801 aagcaccgct tcttttcgtt cttttgttaa tggtgcgaca gtaccaccaa gatcaataaa
    22861 tttagaaatg ttcaacattt cttcaatcgc gccttttgtg atcatctgtg ttttgccagc
    22921 cggatcagcg actacaacac tcatacggcg acgttcaaaa tcaaaaggaa tttcatctac
    22981 tttttgataa ttgattgtat ctgtatctaa agtttctgct gcttcatcaa tgatagccac
    23041 atctaacaaa tttttcagcc ctgtttgata gtagctgttt agataagcat ggcgcaaaac
    23101 acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt tgtcttgtgt
    23161 caatgttccc gttttatccg tacataacac atcgatcgca ccaaaattct ggatagcatt
    23221 taaatttttg atgactgttc cttttttggc catcacactt gctcctttga ccaaattcgt
    23281 cgtaacgatc attggaagca tttcaggcgt taaaccaact gccacagata atccaaaaag
    23341 aaaagcttct aaccaatctc cttttgtaag cccattgata acgataactg ctggagccat
    23401 caatgacata aatttgatca gcagcatgga tgttttgtgg atgcctaatt caaaacttga
    23461 ttggatgggt ttttcagata gcgtttctgc tacatgccca aatagtgtgt catttccgac
    23521 agaaacaatg attccttccg cgcttccact gatgacattg cttcccataa agacaagatt
    23581 ttcataactt gtttcacttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg
    23641 atagctttcc cctgtcaatg ctgcttgaga aatgaataga tcctttgcgg acaataaacg
    23701 catatccgct ggtatcatat ctcctgctga aagccgaaca agatctccac agacgatttc
    23761 ttccatagga atctctgtat actcgccatt tcgtttgaca gctgccgtta ctttcacaag
    23821 tgatttcagt ttttcagctg cttgattaga acgcacagac tggatcaacg tcatcgtccc
    23881 actgacgata accatagtga agataatggc tacaccaaag agatctttat ctcctggagc
    23941 ggctaatacg tattccgtaa tgaatgaaat aattcctaat gcaatcaaca ctaatgtaaa
    24001 gggtgtgatg tatgctttca atatttctat aatgaatggt gtttttttgc catatgaaat
    24061 cgcattttca ccataagttt ctcttgaacg ctcgatttgt tcttctgaca atccatttgg
    24121 tgatgcatgg aacaattcaa ataattcttg cttcgttttt ccagcaaaag ctgcatagat
    24181 tttttgtgta tctttctttt tttccatctc gatctctcct ttgtgtccaa aagacagctg
    24241 ttctcgctgt ttttggtctt ttgctattca tcggatcgat agagaaaatg ctgaaagggg
    24301 ctgagaacag atagataata gccctggcct ttcctctaaa tccttgtttt tcgaatgact
    24361 gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt
    24421 tttcgaatag ttattttccc aactcgctca cctccctttt tgttttttca cctatgggat
    24481 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcaaatca
    24541 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    24601 tctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    24661 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatcc
    24721 tattcggttt tccctcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    24781 tgtttttcat acttttttaa aatacacgcg ttatatttcc tgtctcacgc agttttattt
    24841 gtctcatttt ccacctcccg ctataataga attatagtag gaaacgaggt gctgttatgg
    24901 aacgaacact tgttattatc aagccagacg gcgtaagacg ccatttggtc ggaagcatca
    24961 tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaaatttgca acgatgacac
    25021 ctgaactagc aaaagaacat tatcagcact tgactgaacg tagttttttt gatgagctta
    25081 tcgattacat gacttctggt ccggtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    25141 atatcgtccg taagatggtc ggagcaacaa aaggggcgga tgccgttcca ggtacgatcc
    25201 gaggagatta tgcactgccg gggactgaaa acatcatcca tgcttctgat tcccgtgatg
    25261 ctgcaacaaa agagatcgcc cgctttttcc cgccattcga tacaaacaaa ccgtctgaaa
    25321 aagcggcgaa gtgatacaaa tcctaaactg ttggtctaac tgctgggttt agcaatggcc
    25381 agcagttttt tctctaaaga attcctttga ttcttcccta atgtatattc gatcactgcg
    25441 aaaaactctt ggtgattttc cgatttttct tcccctaaag cagcagaaat cagtcgttgg
    25501 taataatccg ccatcaatgt ttcataaatc actgtacttc tttctttatg cgctaaactc
    25561 cgtttcatat cttcattcac gctacgtgcc ttttttaagc gaccattttc tatataaaac
    25621 aaagtcaagt tcgtcaaaaa agtaaagaga tactgctcgt gattattcag ttgcgcgatc
    25681 cgtcgcattc tttttttgat tttcaaataa ttattctcaa taaaacgatc actgaatatc
    25741 caaggaaaat ctgctagaat agatacttca aattgcccat actctggtac ttgcatcaaa
    25801 tattcttcaa tgatacgaca ttcataacgc aaaaattcaa attccgtctc agatacttct
    25861 gtttttttgg acatgtgcag attcaaaacc aaatagtaca gatcttctgt tttcttatag
    25921 tgcctgcggt tattagcaat ttctttgtaa ggatcaaagc tgtcttcttg aatccgatct
    25981 agcattcgtc tttttttctt taattttttt ggctcatcaa gctgatttcg tatccattca
    26041 tattcggcaa gtgtcatgtt cattcgctct aaaaatgatt tcaatgttcc aaacgatact
    26101 tttttcactg aatttgtctc gatcccgact agcgaagttt tagaacagat tccttccgct
    26161 aattgggtct gtgtcagctt tttgttgact cgaagcttct taatcaattc tccatcatac
    26221 ataatagttc tcctttttta attgaaatta acccttggct tttccagaat gcttgccaat
    26281 aagaggaatg attatttcaa tttaaatttt ccgcattcaa taaatagata cgttaaaaat
    26341 aaaaaataca cgaaaaaaat aaaaaaatgt ttttctcata aaaaatacca accttcaagc
    26401 atgagactgc tgtctaatcc ctgaaggttg gtttgattgt ttattgatca tttttttact
    26461 gctccgcatt ggttcctgtt actggatata ggatagtcgt tgttttattg ccgttttttg
    26521 aaaagacact ggtcgaatgt tcaccttttt gcttttcgat tgcttcatta cgttccgctt
    26581 cgtttttata gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc
    26641 gcaagagatt ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct
    26701 gatctttgga gatttcctcg tcgattcctg ctgctttcgt atcgatgatt tctcctgtct
    26761 gattattgta agcttttcct cctaggacgg tgtatttggg cgtcacgaag ttcccatttc
    26821 gaaaagcaac tacttgatcg tgctgagaag ataataagtc tgtaccaaaa tgaagataat
    26881 ctttatcatc gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccat
    26941 attcatgatt gactgtccct ttagtgtaac caggcatatg gatcatcaat ggcactcgtt
    27001 gcatctgtgc gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcacttgcta
    27061 aatctttatt atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat
    27121 tttggtaaat accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat
    27181 aatgggccgt tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt
    27241 cgaaattctt gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt
    27301 aaaacggctg ttgtagatgt tcaagatatt gggcagattc tctgaataaa tctttatcta
    27361 atatcccata acctaacgtt tcatcggatt catcaaaata agatcgatca aagaaattgt
    27421 cataccctaa atttttatat acatgatctc tattccagaa actagctaca ttcccatgga
    27481 aaacagcact tgtatagcct tctgtttgac caagggtcgc tggtgctgct tgaaagacat
    27541 tatctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca
    27601 acatgttttc agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaaac
    27661 tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg cgtgacttct tgtccgtcta
    27721 ccttcatatt gatcaaaaac tgttgaaaac tttctaaatg aaggacaatc acattttttc
    27781 ctttagcaat cccaaatgtt tcaggatttg gctctgcata atgtttcttt gtatagtcta
    27841 aaacttcatc aattccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt
    27901 tgattccgtc ataaatagtg tacgcatcga ttcctagata tttgacaata taggaacggt
    27961 caaatgtccg ctgtagcaat tggggacggt tggcttcgct caacgctaaa tttcctgaaa
    28021 aaatcaatcc tgaaagacag gtaacagcga tagccatcgg tttgctgaca gggttgcttt
    28081 tgataggtat tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca
    28141 accaatagat gacatcatgg ggtttcatca aagaaaagga gcttcctgac aacccttgtg
    28201 acactttcga aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa
    28261 aaataatatt caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatggcta
    28321 acgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa
    28381 tcgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg
    28441 catagtaggc aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta
    28501 aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt
    28561 ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt
    28621 ttttattctt tctaaagatt attccattca tttttcacag cataaaatcg tttcggtgta
    28681 agcggttaaa atagtaagga gataaaaatc ttgtggtgac cgatttactc tatagtattc
    28741 aaaagtagtt tctcggtatt cgtacaaaaa aaacatacta agattagagg ggaacatcat
    28801 tgacgaatga tgttctcctc tattttctat ttttagatta tagtgaagag tgaaagaaag
    28861 ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa ccgaacttct ttactgtcat
    28921 tttagaatca ggtttacgta tgttggggcc atgagcccgt gtataggaaa ttggaatcga
    28981 aatgaagcag taaagttctt tcaacactta aaaaggagga aaacaaatgc cttacattct
    29041 tgcattagat gtttcaatgg gacacagcta cagtgtccta tacaaagacg acacctgctt
    29101 atttgaagac gagatcgaac acactcaaag aggatttcat gcattactcg aagagatcca
    29161 gcacttgcct gaacgtccat tgattgtttt tgaagcaacg ggtatttatt ccagaccagt
    29221 cgaaaagttc tttcaagatc atcacttttc ttactgtttg ttaaacccgt tagaagcgaa
    29281 aaaacagatg gaagaaaaca ccttacgcag ttggaaaaca gataaatcgg atgcccatcg
    29341 gctcgcacag actcatctta aaaatcaacg tcaaccaaaa gaagtacaaa gcaattttta
    29401 cttagagatg cgcgatttag cccgttttta tcaagaaatt gagaagaaaa tcactcgact
    29461 gcgcatggat ttacacaact gccttcagtt gacttttcca gagcttgaac agttcttttc
    29521 aaacaggctg accccttacg cattgacatt gatccgtctt tttccccatc cagattttgt
    29581 attggcatct actcggacaa aaataaaaaa taaactgatc aacgaaacac gggaaaaaat
    29641 ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc cactatgcac agtgtgccta
    29701 tccagctgtt gaaaaagaca gtattcactg tcaaaaaacg atctattacg ccgaacttct
    29761 ccaagattta ctcgaacaaa aaaaagcact ggcgacacaa atgatcaaaa aagccgaagc
    29821 ctctccttgc tttttgttgt accaaacgtt ccctgggatt ggcgcactca cagccgcttt
    29881 attgctcggt gaattaggag atatcacacg atttaaaacc cataaacaat tgaatgcctt
    29941 tgtcggaatc gacatccgac ggtatcattc aggtaagtat actggccaag accgcatcaa
    30001 taagcgtggt aatcccaaag cacggaaaat tattttcttc accattcgaa atatgattcg
    30061 ccagcaacgc gcagctccta atcatattgt cgattattac tacaaattaa aaaagcaacc
    30121 tatccctaag aaagataagg ttgccacagt tgcttgtatg aacaagcttc tgaaatgtat
    30181 gcacgctatg gttagggcac atacagagta cgactatgcg tacgcggtct cagtggacca
    30241 ttaactaaac cacttacagt ataccacttc tttgcccaga tcgaaaattt tttacgaacg
    30301 gggctttatt tagtgtgcct tgattttgtc gctttcgttt acgagctatt tttcttgcac
    30361 cttttacttg actaatcgta ggaaagaggc tgtgacaaaa ttcttgtcgc agctccctat
    30421 ttcttgatgc aggacagctt tttcacaacc tctccactaa acggtgttca atcagctgac
    30481 cttcggtatt agctcaaaaa acggcaaaat atgaggagct attttcccgt ttttttctct
    30541 aatactcagg tcaaaccgct gatttcacaa cctctgattc acggtgttcc agaagtagct
    30601 tcttcggaaa taagccgaaa ttttacaaaa acttgagaag cacttttcgt aaatttcttc
    30661 ttatttcttg aagctgacca cttctgtcac aacttctgtt tttcattgtc cataggtttg
    30721 atattgtcct tctgatgact cattcgtaat gattgaggct tgattttctt catttcccgt
    30781 tatcgtttgt ccactgccag aatctgaagg gcttagttct gtcgactcgt ttttttgaga
    30841 agaagcagtt gttttcttga cttgttcgtt ggtctttttc cattccgcta gtcgatcggc
    30901 caccactttt ttgtccactt tttcagaata agctgtaacc acttttccat ttttcaaacg
    30961 tgctaccttg tcgccatcat gagtcaaaag caaaacgacc aaatgtttgt ctttgaattg
    31021 atattgtctg agagaaatcg cactaagtac gtcatcgata ttgataaatt gattttcttc
    31081 ttttcctttt ggcaaataga tgtccaattc acctttttcg taattggcag ttaatttata
    31141 ttcctcccac caataagttg agaattcttt tttgaaatta tctactgatt ctttgcttgc
    31201 tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg aaaatgctgc
    31261 ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt
    31321 cctcatgcct tcctccactc ctatttaatt ttgactattc tattatatac aaaaaatgaa
    31381 atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt tcctgaagcg
    31441 gtaagaacgg gaattttcat acattctctc ttggctattc tttaagcttc aaatccattt
    31501 ctgtctgata catctttgaa ccaatgaccc gatttcttga tcgttctttt tccatcttgt
    31561 gctaaatcta ctgaaataaa accgtaacga ttcttatagg cattggtcca cgaccagtta
    31621 tccatgcatg tccacatatg ataacctaaa caattggcac cttcttgaat ggcttgatgg
    31681 acatatttca gatgagattg aacaaattcg atccgataat catcgtggat cataccatct
    31741 tccttgataa atcgtgcttc attttctaca cccatgccgt tttcagaaat aaaacagtcg
    31801 atattcccat aatgatctcg tacattgatc aaaatatcat agattccttt ttcatagatc
    31861 tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc gaagtaatcc
    31921 tcaggcatag gaccgttttt agtatcgatt ggtgtttctt ttgcttttat tcggcgtggt
    31981 tggtaataat taattcccaa aagatctact gtgttttctt taatgatcgt caaatcatct
    32041 ggatgaatct ccggcattag atcgagttct tttattctct caatcaattc actgggaaac
    32101 tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc tattcgtgct
    32161 gctttaacat cttcgggatt tgattcgtct cttggataac tcggtgtcaa atttaagatg
    32221 attccgatca caccgctttg atccatttca tggtaagctt tgatggctaa tgctgaagcc
    32281 aacgcttcat gataaccaac ttggattgcc tttttcatat ctgattcttc cggataatgc
    32341 catcgataca aatagcctgc ctcgactgga acgattggtt cattatgggt aaaccatttt
    32401 tttactttcg agccaaacaa ttgaaaacaa ctttttgcgt atttttcata ggcttcaacc
    32461 gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac
    32521 agattaacaa agggttcgat accgttttca atcaactcat caaaatagtg atgatagaaa
    32581 cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata
    32641 gaggttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc
    32701 gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttcgttt
    32761 ttgtaccaat aatcaaaaat attctctcct tttccatcgc ctgaaattct tccttctgtt
    32821 tgtggaccac ttgctgcaga gccccaccaa aaattttttg gaaattgata tcttttcatc
    32881 aaagcacctc tttttctttt ttagttgatt ccatgtatga tattttccat cacaatcact
    32941 ttttcatttt cttcctcttc ttttttgatt cgtttcaaca gctgttctgc tgctcgttcc
    33001 ccgattttat ctgttggtgg cgtgatcgtg atttttggaa ggatactcag atcgttttta
    33061 tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc ttcttgattg
    33121 aaacattcga tggcaccaat cgacatgttg taattgctga caaaaatagc agttgcccct
    33181 ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc tcctgtaaag
    33241 atctctaaat ggatagacgg atccttttgt accgcttctt tggctccagc taaacgttct
    33301 cttgctacgt aatctgtttg agcggcagca ataaatccta ttgttttatg tccttgtccg
    33361 ccatgatgag caatcacttt tgctacactt tctcgatcat tcactactat tgtatcaata
    33421 cctgctggcc cacccggaca attcaatgcg acaactggga tactcatttt tgtcagcatt
    33481 tcaaatccag accactcttc ttctgataag aagacgatca atccatcgat cgcatgcttc
    33541 atcaggtatt cgattttttg ttcgattttt tctggctgat cggcaaaccc gcttaataat
    33601 aagctatacc cattttcttc agctactctt tcgatccctg aaacaacctc cgaaccaaat
    33661 cggttagata tcccgtttat caacagtcca atagagaaac ttcgattatt cttcaatcct
    33721 ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgatat tttttccata
    33781 ttcttttcct tcagctgctg accgtttaaa tagcgggaaa ctgttcccac tgaaacacca
    33841 gccatcttag ccacgtcacg gatcgatgcc atacattttc actcctgcca gttacatttg
    33901 atttcttttt cttgttgttc gaccatgatg accgctttct ttgcataatg ccgcttgtca
    33961 tcaatatcca tataactaaa gtcttcagaa gtgagttcaa aaacaagcag tctttcctct
    34021 tgagaccgca attcatcttt acgaaaagct ttcaataatt tcgttctttg gatgacaacg
    34081 ccgtcaatta atcggacata gacaagagta cttgttttac ctgaaacact tcctgtattt
    34141 ttaactttaa ttgatattat tactttgtca atatccgttt tgacttccat ctgactgtat
    34201 tcaaatgttg tatagcttag accatgtcca aaaggataaa atggacttcc gcttaaatca
    34261 taataattct cttgtttaga agcagcacgc tgatagtaat aaacaggtag ttgccgacta
    34321 tttctaggat aagacaaact caatcgtccg cttgggttat ttcttccaga aatcatctgc
    34381 gcaatggctt ttccaccctc ctgtccagga aaccatgctg ttgcaatggc attcgtaaat
    34441 gactcgattt tcatcaaatc gtagggtctt ccttgcacaa gtatcgtgat gattggcttg
    34501 cctagttgat acaattgttc aatcagctgt tcttggtatc cacctaaaga aagagaagca
    34561 acatctacat tttctccaga atccatgttg atccctttcg atgaaactgc cccgttacga
    34621 agaaattcca tatcaaagtt ccgcgcactt gatccaccta gaactgcaat gatcacatct
    34681 gagttttgag caagggtcac cgcttctaat aatttttctt cctgatcctc ctgatttctt
    34741 acttcacatc cttgtgcata agaaaccttt gattggggaa actctgtttt tatctcttgt
    34801 actatctttt ttttcaaacg gtcttctgtt tgtggtgcag agtagtctcc caacaagtgg
    34861 taaacatcgt gagcatttgg acctataaca gcaatgtttt ttacttgttg atccaacggc
    34921 aatataccgt cattttttag taaagtgata ctttcttttg ctgtctctaa atttaagtgc
    34981 tctgattcct ccatgagatg atcccaatac tcctttggat cgtttgcaaa tggctgttca
    35041 aataaaccta ataaaaattt gacagataaa actcggcgaa ctgcttgatc tagtagcttt
    35101 tcatctacga cttgattatc aatggcttct gcaatcttgg tataggtctc atcccacaaa
    35161 ctgagatcga ttcctgccgc taatgcatat gctgcagcag tctttttatc tgtaaaaaca
    35221 tcggataaac gatccaaagc cacaccgtct gccataacga ttccttgata accgatctct
    35281 tttcttaatg tttgctcaaa aagcgctcga ttgatatgac aaggtatccc gtcgatgtca
    35341 ttataagcag ccatgacacc tactgcattt cttgtactct tcaataatgg tccgtaaata
    35401 tcggagaact ctctgcgtcc aatcgttact gtgcctgaat tatgaccgcc taaagcttct
    35461 ccttgtgcga tacagtgctt taacacaaca ccgatctgat ccttcttctt attgatttcc
    35521 tctactgtct gatcaagaaa agaaactttt tctgaaataa tctctccttg gaaccctcga
    35581 acgaccgctt cagaaaaacg agctgagaga atcggatctt ccccaaagca ctcttctgtc
    35641 cttccccaac gaggatcttt tgccagatcc aaagtcgaca ccagcgctaa atgaacacct
    35701 ttagcagcca gttcttttgc catcaatcga ctcgattgtt ctaataatgc tacattgaaa
    35761 gtatttccac ggccgatatt cgttggataa gaaatcccat ccaagccttg atgaccatgt
    35821 ggacattctt ctacaagcaa aactggaatt ttccatcgcg aatgctgtag tacatattct
    35881 tggactaggt tagccacctt ccagctttct tttgcaggaa tcccattctt ataatctgtt
    35941 ttagaccacg gatcagagcg aaacagaccg tataaggcac ccagaccgcc tccccattta
    36001 acatgttctt ttaacttttc tgttaattcg atcttgtctc cgtttttttc ataacattcc
    36061 caaccataca aatgctggtt tacctgtcct actttttcag caatattcat cctcgaaagt
    36121 aaatcttcta ctctttggcg gatggttaac tttgggtttt gatacgggta atcgtacacc
    36181 ttaatatcct ccttctgcca attttgcctt attagcggct aatacaaatg gcaggaataa
    36241 aaatacatct agaatgataa gtattactgt taagactgct gctcgtatat cgcctcctgt
    36301 agcaataaaa gattgtataa ccggtggagt gatccaaggt gcagcagcaa ctgttttact
    36361 gatgattcca agacttgtgg ctgcgtaggc aataaccaag ttaatagatg gtataatagc
    36421 gcatggaatc atatatattg gattcatgac tactggtaat ccaaacataa ttggttccgt
    36481 aatattaaat aaacaagtag tcagaccaaa tttagcgatt gatcgctgtt cttgacgttt
    36541 agaagcccac aagatagcaa taactaacgg taaaatacac ccgccacctc cgatcaatcc
    36601 aaatgcattg gtaaagggct ccgtaataat attaggaatt tcttttccta ctgaaaaagc
    36661 atccatgttt tcctgaatag actgtaacaa aggtgcttgc cgtataggag ccaaaatact
    36721 cgaaccatgc ataccaaaca cccataagaa atcagagaaa aactgtaaaa ataagatgcc
    36781 tgggacggat aaaacaaaag ctttcaatgg tgtctgaatc gctaattcaa ttaattgcgg
    36841 aacattcaaa ccagtcgtat ggtttaaagt aaaaacgaca actgaaaaaa ataaaatgac
    36901 aactatttct ggtactaaag aattaaatga cgcagctact gccggtggaa ctccttctgg
    36961 catcctgatt ttaaaactct tcattttcgc taatttgata aataattctg tactgacaag
    37021 ggcagcaatc atcgccgttg ctaagccaga cgaagaagtc agactttgta agaaaacccc
    37081 tgtagcttct gctgtatttc cgtccatcaa agtgactgta cttgataaag acatcaaaat
    37141 aaacattacg gctgtactta gaattcctgc atgaacagga ctaaatgatg gatcatcaat
    37201 cacgcctgtt tggatatagt gggttgccaa attccagccg atattgtaac acacgataat
    37261 agctaaaata ctcatcgtac cattattcac gattgaagca agttcagcca atggacttag
    37321 atcaagtatt ctttggactg ttgcatttga aaaaatcaga gcattcagca aaaccatgac
    37381 agaagcagcc attactaacg gcatagtaac gataaaactg tctcgaatgg ccagcaagtg
    37441 tcgctggcta ctcatcttag atacgattgg tagcattttt tcagatagtc tgtctacgac
    37501 tttcattatt tcatctccta gattctaaaa ttgaaccggt tcatttttaa tatagcactt
    37561 tttttaaaga ttgcaacaca tttttttaaa acgctttcta aaggtggtgg attttttatg
    37621 acttttgaaa aattattcta tttaatggta ttttgtgaat tttttctctt ccatccgttt
    37681 gaagttttac tttccaatgg tataatttct ttatcctatc aaatggggga agtattttga
    37741 agaaattaat cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt
    37801 tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg
    37861 aaaaagcaga tatcaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat
    37921 ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac
    37981 aaggagctga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatta
    38041 ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa gcaacagtca
    38101 catttaccaa aatcggtaat taccgtttga aggataagga tggcaacatc tacagtctat
    38161 agtgccatct gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc
    38221 aagatgactg taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat
    38281 aacaacaaaa atatccatac agaatttaat gatcaaggaa gtttgagtat atgcgacaat
    38341 tgccacaaac tagtcaaaga ttcttttaaa tcgtttgaat cttatcctgt caaaaattat
    38401 gtaaaaagca aaagaagata agcaaaatca agcaaaactc actatggctg atttttatga
    38461 acttcaaact cagcttcttc ctttgatcaa gcttcaaaaa ctggaatcag taaaacaaac
    38521 agtggatcat ttcagttcac aaatcaaaga gtaacgtttt atttatttca catctttgaa
    38581 tataaaataa ccaaacgata gaaagactta ttctacatca cgtagtaatt tttctttccc
    38641 gtcgtttggt tttttacttt tagcctaatt cttgaccttt cgcatgctga ttatctgtaa
    38701 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacgaatca
    38761 ccttttttgc atcttctgga ttttctacaa aatcgaatgt atccccatcc acttgtattt
    38821 taggacatac atcaaaatcc tcataccatt gattatagcg ggtgttcagt gtttcgtaat
    38881 aggtgtataa cgtttcatcg ctttctagct gttcatagtc acgcccgcgt tttttgatac
    38941 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcagga cgtttttttg
    39001 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    39061 cgatatccgt tacccgacct aaatcagcat ttagatggaa caacagacta tcttcgtaga
    39121 tggagcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    39181 tattcaaaaa gaagatttgc aataaaaatg cgtactgctc aggatttgaa taaaaaagcg
    39241 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    39301 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    39361 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatttttcaa
    39421 aatcatattc acgtacagct tggtctgatt ttaaatacgt gtgaagactt tgtgtaatca
    39481 tcaagttcgt aggtaccgtc tcttcttgta cgatgatcat cggttttttc aacatagttg
    39541 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    39601 ccaaaacaaa ttctgcattt gtcaactctt ccatatcttt ttcgtatact tcctgcgccc
    39661 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    39721 tgattgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    39781 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    39841 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    39901 gatcacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    39961 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tatcaggtgt
    40021 caggccgatc gctatccaag aaagaagttg cgaaaattcc tccaatgtac ctagacctcc
    40081 agggaaaacg aggacagcat ctcctttttc aaataataat acctttcttt catcaattgt
    40141 ctctgtagga ataaatgtag taacatcttt cccaggcagc tcagtttcaa aaaggccatt
    40201 tggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    40261 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tatattttcc
    40321 taacgtttgc gcaataaatt tgtaactttc tttattgcca aaacgggaac cgcaaaaaac
    40381 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    40441 tttttaaaat aaaagactag attttgtagt tttttagtgg tatatagatc aaacaccact
    40501 atatatagta tttgcaaaaa aataattttt cagctatttt acagtaacca aaaaaataaa
    40561 taactagtaa atataaaatt gaaacgactt acttatagaa gaaattggga tttcgtcctg
    40621 catgtcaata acacgcatta tttaaatagt cacacacttg tttctcctat taatgaaaaa
    40681 tccattttat tctcgtgtta tgtttcacca acgcctgatg tttctctaaa taaaaaagcc
    40741 gacgaaccaa ataatcgaaa tttgatcatt ttggttcatc agcttgttcc caaacctttg
    40801 tattaacgat aaatagctgg attcgtaaaa ctatcacttg gatcatcact gctggaagaa
    40861 aattcggtga taaccgcgcc tgttgcacct gcctggaacc agtgtttaat attttgttcg
    40921 atcgtgtact gatctccagc gtgaagtatg atttcttttt ttgccgtata gtaacgttct
    40981 tgcccctttg gaataataca ggaaagtttt tttgtttctt ttccttcgac ataaagatat
    41041 actaagcctt ttcgacaacg aaaggtttcc ttttttcctt cattctcatc tcgaggtggg
    41101 tgccgatgct ctggacaagt ttgataaggc aataatacca gttctttccc acagtatcgc
    41161 tcattatttt cataaacaaa taaattcaat ccttcagtct cgattgctcc taatccaaaa
    41221 tctgtatatt cgattctttc taattctgtt tctttcaata ctactcctga atctaggaac
    41281 atttgttgta ctcttttctt gtaatccatt ctgttcctcc taactgattg tcaagtcatc
    41341 ccaagatatc tcttcttcct ctttttcttc ttggatgact tctgcatttg ggtcaactgg
    41401 ttttttcaat aataaaatag ttattcccgt agcaaaagaa ccagctaaca atgcaccaat
    41461 gaatgcccat ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct
    41521 atctgcgccc caaaacatgc tcaatccacc gccgactgcc ccaccgactc ccgttgctat
    41581 gacattgcgt gccaagtcgt tcaatacgat agggatacat ccctctgtga tctgacagat
    41641 ccccatagga aaagcggtct tcaatgtatc gatttcttga cgattgaata tatttttacg
    41701 aatgattttc cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa
    41761 aatcaataca gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc
    41821 agaataaatg gtaatccaga aacagccggg tatctgcggt aataagccta accaaacgaa
    41881 aaatatgaga agctaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca
    41941 gggcagcttt ttcacaacct ctttttttat ttatgtttca taatcaatat ggtaaggatg
    42001 cagctccgta tggtagaccg cctccgaata ttcaatcatt tccccaattt gcgtagacga
    42061 tttccgtatc cgtttgatgc cgatctgtgc gtctgttttc agaatttctt gttcttcttc
    42121 tgttaaaaat actgccgaga aatcatcaga aaatgagtca atagtatatc cttttttcgt
    42181 cagcaaacga tacaaagatt ctttgccaaa ttcttctctg ctgaattgca tcatttcttg
    42241 tggaagataa tgaacaaaat aaatataagg ctgattgttc aaataatata agcgcttaaa
    42301 acaaacagct tgctcaccga aaaaggaaaa caaaggatct tccttcttta tatggaccaa
    42361 ttgcaaatcc agcgtttctt tccggacttc atatcgggag ttctccaaga tttgcgtaaa
    42421 gcttgccgct ttggataatc gattgtaagg acgattgctt agaaccgtcg tcccacgacc
    42481 acttttcttt gcgacatatt catctgatgc cagcagttcg attgcctttc ggacagtgat
    42541 tttgctgact tgaaacagtt cttctaattc actttctgtc ggtaacatcg aaccgactgg
    42601 atattttcct gagaaaatat cttctttaat tttatctgcg acttccaagt atagtacacg
    42661 tgtccttttc ataacgtcac cctttgcttt actattacta aaagaatagc acataaaaaa
    42721 taaaaaaaga acacaaatca catgtgagaa gtgtgttctt tttcattcag ctgctttaat
    42781 tacaagttta cgccatttga ggcaattact tctttatacc aatcaaaaga ttttttcttt
    42841 gatcgattca gtgtcccatt tccttcatcg tctagatcca catagataaa gccgtaacgt
    42901 ttactcattt ctcctgttga tgcactgaca agatccatac atccccaagg cgtatagccc
    42961 atcaaatcta caccgtctgc tactgcatta gccatcgctt cgatatgacg acgtaaataa
    43021 tcgatccgat aatcatcaat aatcgtgcca tcctcttcca tatgatcaat ggctccaaga
    43081 ccgttttcaa caatgaataa tggtttctgg taacgattgt ataattcatt caatgcgatt
    43141 ctcagccctt ctggatcgat ctgccagccc cattcacttg cctcaagaaa cggatttttt
    43201 actcctccca gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc attgattgcc
    43261 gtagacatat aataactaaa tccgatgtag tccactggat attcttttag caactggaag
    43321 tctttttccg tttgctctaa tttttccgga tcgactccat attttttatg ggtacgcgct
    43381 gtgtatgcag ggtattctcc acgtacttgg acatccgtgc agaaaaaatt gaattcttgg
    43441 ttttggatca ttgttgctgc ctgattgact ggattggcat cgatgctgta agttgttgca
    43501 tagataatca tgcagccgat ttggatatcc gaacgcaatt cgtggccgat ttttacggct
    43561 ttgctacttg caacgaattg attatgccat gcttggaaaa tattttggta ctctcctgct
    43621 ccattgcttt ttactaatcc ttgacttaaa gctggaaagt gaaaggccga attgatttca
    43681 ttgaaagtca tccaatattt gactttactg ctgtatctct ctaatactgt ctgagcaaat
    43741 cgttcataga agtcgatcag ttttcgattt ttccagccac cgtattcttt tgccaaatga
    43801 agaggcattt cgtaatgaga aatcgtgatc acaggttcaa tatcatattt taagcactca
    43861 tcaatcagct gatcataaaa ttctaatccc gcttcgttag gagtaccttc gtctccattt
    43921 ggaaaaattc gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac
    43981 aaggcaagat cttctttgaa acgatcgtaa tgatcaattc ctcgatgatt cgggtaaata
    44041 tatttttctt gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc
    44101 ttacctccag gcatagcatc tgcaacggat agcccttttc cgtctcgatc ataagcccct
    44161 tctaattgat tggcagcagt tgctccaccc cataaaaatt tttctgaaaa acgtgattgc
    44221 atcctgattt cctccttatt ttacaattgc cagaacatct tcgtttgcaa taatctcttt
    44281 ttggtctaat tcaagtatat cggcaaatgc atcagtattc gtaatgacaa ctggagtgat
    44341 tacgctatag ccagctgctc taattgcttc gatgtcaaaa cgaatcaata aatctcctgc
    44401 tttgacgaga tccccttgtt tgactaagat ctcaaagcca ttcccattta attctactgt
    44461 atccatcccg atatgaatca aaatctccgc tccgtcagtc gttgtgatcc ctacagcatg
    44521 tccagttgga aaaatagtcg tgatctctcc gtctgcaggt gcaactagtt ctcctacttc
    44581 tggatcaata gcgatacctt ttcccatcac gccagaagaa aaaacttggt ctggtacttc
    44641 actcaatggc agtactttac ccgttaaagg actttttaag gtatgccgtg ctgaagtaat
    44701 cggttctcct gcattcgcat gtttgttttc taattgattg tcttgtgttt gttcgtcaaa
    44761 tcctaaaatc aatgtaccta cgaatgcaag aacaaaagca attcctgtag ctattactgc
    44821 tactgtcaca tttgactcta caccatccac cgtattaatg aaagtcggaa tcgtcaataa
    44881 gctgaccaaa ctgctggaga aagcttttac tccggaaaat ccgataatag ctccaccaat
    44941 acctccagaa atacaagcgg caatgaatgg tttcttcaaa ggtaatgtca caccatacac
    45001 agttggttca gtgattccaa ataacgaagt cactgtagaa gaaatcccta atgctcgaag
    45061 tttcgtatct tttgttcgca atgctacagc taaagcagcc ccaccttgtg ccaagatggc
    45121 tggaagaagc attggcatca atacatcaaa cccatattgt tcgatattca aaaacatgat
    45181 cggtacgaat ccccaatgca tgccgaacat cacgaatact tgccataaac ttcccatgat
    45241 tagacctgct acgattggac taaatccata aatcgagttg aagagaccgc ctaaagcatt
    45301 accggcaacc gtcccgattg gtcctaaagc aagaaatgtt aatggaacca tcactactaa
    45361 aacgacaagc ggaactaaaa tcatttgcag aaattgcgga atcacttttt ttacgaatgg
    45421 ctctaattta ctttgaaccc atacggctag aatgattggt atgacagaag aagtataacc
    45481 agatggtgaa agaatcactg ggatcccaaa gaaactgata gcttctcccg caccagcaac
    45541 tgctgtgata gctggataca caagtgccat agcaattgct actgctaaaa actggttggc
    45601 tttgaacttt ttagctgctg taaaagcaag catgacaggt aagaaggtaa agacaccatc
    45661 tgctgcagca aacaatattt gataagtacc gctatcagct gtcagccagc ccatcaccgt
    45721 tgccaatgat aagaatcctt tcaatacccc ggcggctgcc atcgccccta gaaaaggtgt
    45781 aaatatacta gagataatat cgatcagacg attcaaaata tttttggact cttcttttcc
    45841 atcctccgaa actgcaagac cttcctcatc cacgatcgat tgataaacat ctgctacatt
    45901 actgccaatc actacttgat actgaccgcc actttgtacg acttggatca cgtccgggtc
    45961 ttcttttaac ttttgcgtat ctgctaaaga ttcatctttt aatctgaatc gcaaccgcgt
    46021 agcacaatgt actaaacttt tgacattctt ttgtcctcct accgcttccc atacacgacg
    46081 accaactgct tgattatcca ttgttttttc cttctttctt ttttatttaa aaaataaaaa
    46141 cccagattga aaaaaacagt cacaaacatt cgtgaaccta ttttttcaat ccaggttttg
    46201 cctgccatac agtaacaatc cttatgattg gatgcttttt ttctcactta ctactctttg
    46261 aatatgaatc gttaaataaa tctgctctgc gatccccatt tcctgttttt gcgtttgtcg
    46321 aagatactca ttgatccgtt cgacacaatg aaacgctttt ggatactttc cttgaactaa
    46381 tgcgtagaga aattcatctt gctcgtcaga tccttcccct ttgagataac gctgggcaaa
    46441 aaactgcaga tgagtaatga tacgctgata attcaaagat tcttcatcga acacgatgcc
    46501 gaagaattta ctgatgatac tcagtatatc tcttaccatt gtggtagcgg tcatcgtcac
    46561 ttgcatgtct tcgtttcctt gttggctatt cacaaaatga aaggcaataa aaccggcttc
    46621 atcctcagaa agttccacac ccgtcttttg tttcacgaac aaaagagcct ctttcgctgc
    46681 ttgatattct ttcggataaa atttttttgt ttcaaataat aatggattag gcagttggat
    46741 attttctttt gctcgtgtga tcgcataatg aagatgatcg gtcaatgtca gataaatatt
    46801 aggcgaaaat tcaactttta gtttatcttc agccagttcg actacttgtt tgacccattc
    46861 gatctcctct gccggaatat cagccatcag ctgctggaat tggtttttag caaaagaatc
    46921 tcttaaaaca aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg
    46981 aaaagctagt ccttttccca tacaaacgac ttcttccccg aatccatttt tcgaaatgac
    47041 gacattgtta ttcaaaattt tatcaatttt cattttgctt cgccctctca agaaaaataa
    47101 aaaaacctag aattcgccta ctgtttctta gctgaattct ggttttgcct gccgtacagt
    47161 aacaatccat ttcttattca aaatcatcat accatcgaat ttttatttat gcaagccttt
    47221 tcatataaaa aataaaaaaa ctggaacaca agtctatcta gaaaacaacc aaatcagaga
    47281 tgcacctctt tgcgtttctc tttttttcag caaattggtt tttccttatc gattcatatc
    47341 ctttggctgt ttttaatctg taacgactta tgtcccagta tcctaaaatg tttttgtctc
    47401 tagattgaaa gcagatctca tatcatctgt cgcatctttt atcgatgtaa cactttccag
    47461 ttctccttgt acaactagtc gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac
    47521 aatttctttt ccctcgactt cattcagtaa ttgggtatct gtaggagcaa ctttttcttt
    47581 aaaaaatgtt ttgtatgtat acacattttc taaatctgtc aaatagatct tggcacccat
    47641 ctctaaattt tctagcggtg taaacagcaa ttcagggttc gttgcacgat ggcttgctaa
    47701 ggcataattt ccttttccca tttcttgatc tggagataat gttcctgctc cgtaaagtag
    47761 tgacgtatta tccaagcctt tgaaaaccgg taaattgatt gaaactgaag ggactgcgat
    47821 actagcaatc actggtagat ctgttcctct catttgcgac ctcatgaccc cttctgtcgt
    47881 cattggttct accgcatcaa agtcaaaact gacatcttta tcgttattct ttttcagatc
    47941 ttcttttgtt acattcgcta tagcatattt atctcctgtc tctctgacaa aataatcctt
    48001 gatctgttca ttgaaaatca aagctagtcc tactaataac aagaggaaca agaggatatt
    48061 gactagccat ctttttgctt ttcctttttt tttcattttc tctccctcca gactcatttt
    48121 actgtttttc ttttgctctt tccaacagcc gaatgacaca tttctacaac tctgtgtatt
    48181 tttttcgtac acctgcgctt ttttctacag gatatttcaa attatttttg atcaattttt
    48241 cttcaatgat ctcatctaga tccaggtgaa ggttgtctgc catcatgtat gaatagatca
    48301 atacatctgc cagttcttct ttgattcgtt ctaattgtgt ttcttttact tcttccggcg
    48361 ttttccattg aaaaagttct aacaattcag atgcttccaa agaaatagag atagccaaat
    48421 ctttttcgtt atgaaattgt cgccaattac gttcatctct aaactggttg actttttcca
    48481 tactattcat ttgcttgcct tcttccaatc acttcttact tgattaatca taccattttt
    48541 ttacataaaa aatagatgat gcttttaaaa aaactcctgt tttcttttat ttaaaataaa
    48601 tgtcatagaa aatcaataat ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc
    48661 ccttgaggct ttttgttttt cgatacgttc ttcttcccaa acagacaacg ctactagaat
    48721 ctctcttaat gattttcctt cttcggtgag cgagtactct actttaggcg ggacttggtt
    48781 ataggctttt cgatggatac tttcatcttg ttctaactct cttaactgct gggtcagcat
    48841 tttttttgtg atcgtaggga tttgcttgct tctccacgcc taaaataagc gatattgctc
    48901 ttccctttat ctggtgtaag caaagcagtt agcattttga cgatcgtggg ttccgctttt
    48961 cacttcttat ctacttagta tcatgaagta tactttcgta ctaatactaa gtagcggaag
    49021 tgtcaactac tttgtttaac tacatagcaa aagaaacaga agggtgacaa cggtcatcac
    49081 gcttctgttt cttttgaata tgctgtgttg ttgtctctat ctccgtgtag actttcctca
    49141 aacttatcct ttgacagtaa gtcagtaggc tgatttgctc tttctagatg ccagatacat
    49201 ttctacaaaa aaatagtgat tctgttttga aaattcgatt tttagttctg cattttcgta
    49261 aacatccaac atttttttcg cactgtatag acctaacccg ccattttttt tagaggtttt
    49321 tcctcttttg accatctccg atagcgtcaa attcgttgga tttgccttgt tcgctatact
    49381 aaaacgtgat ccctcttttt cttccagcag attgatcgta atcggactct gatcggctac
    49441 acgatgatcg aatgcgttag aaataaaaat agataaacag cgaataaaaa caaccagatc
    49501 aatcgggatt gttttcactt cgacaggaat aactacttgg atcgggattt tctccgattc
    49561 tgctttttct agaaatttta aaaagacacc cctgacagct ggggatacaa tatttgttag
    49621 ctgttgtatt tgcggttttt caaaaatagc tgcagaatga tcttctaata tttctaaaaa
    49681 ttccaacgtt tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt
    49741 ataatcatgt ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg
    49801 aagcgattcc tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa
    49861 caaagaaaaa aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg
    49921 gaagtgaaca cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc
    49981 tccaaaatag tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc
    50041 tatacttttt actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt
    50101 tccaagatgt ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc
    50161 agtgatagca aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa
    50221 ataaaaaaga aacatcaata ataagccaat taggtaagga aagctcactt tagggatagg
    50281 aagtaaaagt gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg
    50341 tttcgaaaag aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt
    50401 cactgttgcg accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt
    50461 aataggagcc tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa
    50521 tggatcatca gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc
    50581 tctcaaatca atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc
    50641 gatatcgact tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa
    50701 aatgcacata gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc
    50761 aatcggaaaa tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt
    50821 cgtcaagtgc tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc
    50881 tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa
    50941 agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg
    51001 aatacttctt taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta
    51061 aaatcttact gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta
    51121 gaatttttca acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa
    51181 cgtttccatg acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca
    51241 tagcagattt cagggagtgg gccttgaatc ggctccactc cctgaagtcg ttgatatgat
    51301 tttacgcttc ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt
    51361 tgtctacagc ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca
    51421 cattctctcc taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta
    51481 gatctactgc ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag
    51541 atggcattcc actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct
    51601 ttgaaccagt tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg
    51661 ataatttcca accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt
    51721 gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg
    51781 tacctaactt gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg
    51841 atgttccctg atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag
    51901 gaggaactgg aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc
    51961 cttctgtagt ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac
    52021 ttccagctag aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttcttt
    52081 taaaataaat aatccagcaa ataagccaat aagaatgact ccaacaagta tcagccatcg
    52141 ccagtcaaat gtttctggct gattttttaa atcaatatcc ttttcattca attggtccgc
    52201 tgcatcatca gttatttcaa aatcggtatg gagattccag cgtttttctt tccaagcacc
    52261 cttcacaacc gcttgataat gtcccggagc taattttttt ccttctaatg ggacaagtaa
    52321 atcaaaattt gtattgggtg ctacatctat tttttctttt tttattgaaa ggtaaggctc
    52381 ttttgtttct tttttgtaga tttcaaccga tgtagtcaag ccgaacatat aagaagcatt
    52441 tggattttga agattcgcct taatcacatt tcttccatcg ctttgatccg cataagcttt
    52501 ttttaatttc aaatctgggg aagactcttt ttcgctattt caaatcaaga ttgctttcac
    52561 ttgagaaaac tgattgacga caccaactgt acttttgtta gaaacgttct cttttttttc
    52621 ttgaaacgtg atccctcctg cgaccacacc agccagctct ttttctggcg ctttcagtga
    52681 aagtgtctca gtgaccgcac tttgaggagg aacagttatt tctcccactg tttttgctaa
    52741 gtttttcata tcataactta atgtttcatc aaatttactt tttgattctc catattcgat
    52801 cacaccgttt gaattcgtcg tagcggagtg gatcaatgtc tcaactgtta ctgatttatc
    52861 tgttcggtta aagaccgtgt aggggatttc ctgtgttttc ccaggctcca tccttacatt
    52921 tatatatgtt ttgttcttat caatctgatt agccggtaat tcaaaatcta ccgcaaaatt
    52981 gaattcggag gcatggacag gtgtctgcca aaggatgaat ccaaggagaa ggatcataag
    53041 taaagagcag ttgtttcgat gacttttatt ccatattcga ttcatcaatc atcctccttg
    53101 atctttattc tgtcggcgca tctgctaatg tctatgttat ctctgctgag taattaccag
    53161 ctatctgatt ttctttggtt aagacaagtt taaacgcccg accgttattt aaatattctg
    53221 aataatctgc ttgcacattc gttgaatttt gtcctctgac aatttccagt tcttcttcat
    53281 tcactaattg atactcctct cctttcttga aatataggac atcctgcata gaaaatcctg
    53341 catcatttgt gaaaggctga gataatttta tcgttaagat ccacggattt ttcgtttctc
    53401 gctgatcata aatccataat ttttcctgta tatccatttg gccaaatgct tcttgtgtca
    53461 aaccagttgc tttgatctga ccaaaatcga gtttggaagg ggcaagaaat gagagaccac
    53521 cttctactac gatcttcgta gcaggtgcaa aaggcggggc atcatgataa acaactggcg
    53581 tatcacttgg attgtgattc ccaatttcat cgttggtact tggcttgttc tgcacaccct
    53641 tagcagtagc ttctcctgct tggtcgttta atacaatttc taatacatct cctgcaaaaa
    53701 gttgattttc tgggatagaa agaacaaatt cccctgtatc atcaatagtt gtttctgaca
    53761 aaacagcttg attttttttc aatgaaacca aggctcctgc ttcacctgta cctgtgatcg
    53821 tgcgagaagc tgcattgatc aatgtggtac ttaaattagc aggttcagga ggaatcacat
    53881 cgactgtcgt tttggcagag tccagttctg tcacttcttg gccacctctt acttttttag
    53941 cagcaacaac ttgcacttga tcctccgttt ttaatctctc atcattgggt acttgaactt
    54001 cgaacaagcc gccttgttcg ccttctcccc atgggttata tcctgcccct tcattttcat
    54061 ctgtttgtcc ttgcgtgaga gcagtaattt ctacagatga tttcgtcgta tctgcgtaag
    54121 taatacgtaa agtcacttct acttcgcctg tccaagcatc tcttggcttt gcctcgacac
    54181 cttcgggaac aacgacatgc ccatagattt ttttatctgc attcgttggc actcttaagg
    54241 attcaatcat cgggagctga ttattggcac tgattcgatt ataatttttc attccggcac
    54301 ctgtctgagc aaattcattt gttaaacctg cactagggct tgatacgaaa ttgcctaagt
    54361 cggtcccatt gatcgataag tctgtcatat cagaaaaata atccgggtct ttttcaaagt
    54421 tatcgaatgt tgcgcctgtt tttcgccata aagcgatttc tgaatgggcg atctttaaag
    54481 aagaatctcc atttccttgg aatacccagc cgcctatgcc attggtctcg cttccaatcc
    54541 gtttgttttg gaaatcaaaa taggtaggag attctaagtc cacattcaac acatccccac
    54601 tgatgatgcc ggtcttatct tcccctgttt tcccgaccat gactaaataa gttttttcac
    54661 ctgccgtaaa atttaaatca atatctccag ttgtatctac agttgctccg gaatctgcgc
    54721 gtatatcgat atttgatttt tctccagtga ctgtaaaatc agctgaacct ggactttcta
    54781 gaaaaccaga agcgtcataa aattggatcg cttgatttcc ttgattgagt ctgttcacgt
    54841 aagatttgcc atcacctgta tttcttaccc tcaatgagcc tccattggtt actgtcagct
    54901 tttgcgtacc attctcaaaa cgaactgcgc tggaacgccc actctgctta tcgatgtcga
    54961 taactgatcc ggctccttct acgatcaaag aaaggccttt tcctacaaaa cgcatcccat
    55021 tgttcccggg ttgatcgtca tctccagcca ttttgaccga gatttgtcca ccacttgata
    55081 catggatttg actagaatct ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg
    55141 tgattttccc accattcgat acagtgactt tcgatcctga acctctcatt tggatgattc
    55201 cgcgattgtt gtcattggta atattgatct ttgtatttac cgaaaattcg cttcccgaac
    55261 cactgacttc aaacatgaca ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa
    55321 cgccatctgc ccgatcataa tcaagagaga cctttgaacc gcttaagata tggattcctg
    55381 gcgctgtttt tccactacca gtctcatctc ctgaatctag aaaaccgtag aatttcccta
    55441 gatacggtcc ataaagttga ctaccattga tcacattcaa ttgatagctt ctacaaaaat
    55501 aggctttacc cggagcggca ttcagtcctt ccttcactgg ttggacatcc aacacaccgg
    55561 aaacaccatc taatgtgaca gttgcacgag gagcgtaaat cagtcctaat ttggacccat
    55621 catacaaatc aagtgtcccg gtaaatttta tttctcccgt aaaatgatat ctgcttcccc
    55681 aaaaatcggt catataagca atgataatgg catttccatc gatatttggt gtcaatccac
    55741 ctggatgtgc agctgttcct ttaaaattct ggaactcgat cgactgaggt atctcattga
    55801 tataaatatg gtgtgtgccg aaattgtgag cgaatccacc aaaatcaata acgatatctc
    55861 gtttgatctc taattccctg tctaaagtca aaggctgaat cagtcggatc ttagttactg
    55921 attcgtctgc gagaagctgc gccagttctt ctgttgtggc agcttcgctg tacccatccg
    55981 ctgaaacagc tcttgccagt gccgcttcac tgctatcaga cgtttgttca gctacttctg
    56041 aggtctcact tgtcttattt tcttcttgcc ttttattagt agaagcctca ctttgagatt
    56101 ctgtagtttc agaagtaatc gattcttctg aaaaagtagt cttgtcaaaa ctttgattct
    56161 ctgattgcgt tgattcgttt gttttctcta catcggcaaa aacggatgtc accatcaaga
    56221 gagggaagac aaacaaaatc gacgcaatca gcttttttac acattgattc attctccacc
    56281 atcctctctg acgttttgac aattttattt tatcgtttta atctatttcc aattgtactt
    56341 tcatatttaa aatatgtatt tttatggaaa aatttttcta ttaggtgtaa atttcttttt
    56401 tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt
    56461 gttctcccgc caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag
    56521 ggcaatactt tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc
    56581 aattcttcct catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt
    56641 ttcgtagatt catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa
    56701 gaagaatctt ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt
    56761 aatgtttcaa aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt
    56821 tcttccttct ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt
    56881 ttgattcctg acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca
    56941 tcattgattg attcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt
    57001 atttttccaa tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca
    57061 ggttctagcg gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat
    57121 ccgaaatttg gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca
    57181 tctccgccca tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt
    57241 tttgcatccc ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat
    57301 tttgctatcg ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc
    57361 actaataaaa cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga
    57421 tagcataaag aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt
    57481 tttgacaaga taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga
    57541 aataaaagaa aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata
    57601 aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct
    57661 tatagcgatc agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat
    57721 gtcacggcct tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa
    57781 aacggaaaat tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt
    57841 atttgtgaga aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca
    57901 aaaccactcc atttatctac ttctacgtgc attttttgat attgttttct ttatttaaga
    57961 ttatacagaa aaatatctag ggtcgctgga ttctaagtaa aaaaaatttt taaaataaaa
    58021 agaactaaat caagtagtaa ttaaactact gttgatttag ttctaataaa aagatttaga
    58081 tgaacagttt tattgattta aatagttcat attttttatt tataccgctt atttactact
    58141 tgctgtacta gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat
    58201 ttttacatta gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact
    58261 taattttgtg tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc
    58321 tttgttttgg tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt
    58381 ggtagaagtg atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac
    58441 ttctgccgga accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt
    58501 cgtagtatca gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc
    58561 tttcttcaca tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc
    58621 tggatggaaa gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt
    58681 tagaccttct tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta
    58741 acctgcagat ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata
    58801 ttgagaatcg atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata
    58861 aacgatcaac tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac
    58921 ttcttgttcg ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt
    58981 acagcctgac aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat
    59041 ttatcaattc tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg
    59101 aaaatcatca agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta
    59161 cacaatccag aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat
    59221 actacttctt ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa
    59281 ttcttagaac aaatgttatc gattttgttt ttcttcttcc gctgctggga tattcaaatc
    59341 agacactttt atgttcgatt ttccagacat ttttttgttg tttttattat gatatatttt
    59401 tatacaacta aaaatgaata tattaaaact accaaaaata acttttaacg ctgtttcatt
    59461 atatgttttt tctattttat ataaactttt ggatacttaa taccaaccgg ttggattgta
    59521 ccaacatgag aatgtgtcag ttctatttcc tgtcgcactt gcataggccc atactccttt
    59581 tgttgcattt gctgatcgaa tagagtttcc aactttcgct tttgcaccgt gacgattata
    59641 gacacgacta tagtggttag aatgacctgt ttttctcgta caagttgtat cactataata
    59701 aacccatctt ccagagctat gattaattgc tgttctagaa atttcccggt ctactggtaa
    59761 atttgactta ataatattac ctttagcatc aaaatgaatc tctcctggct ttaaagattt
    59821 gtaatagatt tcaccgtgaa ctgaatttgc ttccacttca gatgacatag cgtttcctcc
    59881 aacaaaacct atccctagtg cacaaataat aactgctttt ttcataaaac gacatccttt
    59941 cctttttgat ttttaaagca tgatcttctt gtttcctatt ttggtcagat atggatcatg
    60001 cgtgactacg ataactaacg taccttggga agcttgttgc gataagattt caactacttt
    60061 atccttattt cttttatcca atgacccagt cggttcatcg gctaaaatga ttttgctatt
    60121 cttcaataag actctcgcac aggcgattct ttgcttctca ccaccagata atgtgtagat
    60181 aggcgtactt agcttcttgt ttatatttac ttttttcaat gcctcatact ttgctgttcg
    60241 cttatctgaa acatttaaat tcgaaaactt caatccaata tctaaattaa aatcgattgt
    60301 ttcatattca atcaacccaa aatcttgaaa aagaaaagat atggtatttc tttttatcaa
    60361 gcgcttctct ctcccagtta attgtttcgt ctcttgatcg aacaaataga attgtcctga
    60421 actgaactga tcattcaatc ctaatatatt caataacgta cttttacctg ctccactttc
    60481 tccaacaaca gtcacaagat ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt
    60541 tttgtttcga atgatttttt tcgcaccttt tatcactatc aacggctcca attgttaacc
    60601 tccattcatc agcaataact gacttttctt ttctgtagac aaaataaaaa caactgaaag
    60661 aataaaatca atgaagctaa agaaaaaaat attaaaaaga tatttaggct ctctttctat
    60721 aagaaaaagg aagattacac ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc
    60781 tttatacgtt tcaaagagcg aataaccact tgaacgaaag atagcgattt tccgacaatt
    60841 gtagacaaaa tatatgcata acatgacata gctcaacaag aagaaagaaa agaaagtgag
    60901 taaaaatatc tgtatcaagt aatgaaatga tctgcttaac actttgacag ctatctcttc
    60961 agtctgagta tacggaaatg ataactgcaa gttatcacgt aactgacttt cttcaatcag
    61021 ctcaaataac ctttctttcg acaaagagcc tatcttgatc tttaacggtg gatagatttc
    61081 tccgctgaga atatttcttt ccaaatacat actgtttttc aatgtcagta tttcaacaac
    61141 cggataatgt tcgatccagg ggatagaagg aataaatgta taaatgggtt gattgttttt
    61201 tataaataga aatttcggtt ctttttcact gatttcttga taatattctg ttatttgcgc
    61261 caggtcattt tttaaatctg tatgtctata tcgttcaggg atcaatataa cagggtgcgt
    61321 ttctttttcg gttatttgga tcaaccttcc agagacatca atcaaaggaa atttcttaag
    61381 ataattcaga tttattttta tccctctgcc aaactgttca ttttcttcat tcaagtaatc
    61441 tgatacattg actaatatac ttccgttttt attcaaagtt tcatataatt cctgatctgc
    61501 tgctgatgca aaacgattgg aataaataaa atccacgtgg tttttcccga tggttactgg
    61561 ataaaataca tgatagtcct caccagcgat ttcttttggt ggttcatggg aaacagctaa
    61621 ctgtgaagaa gaaaaaacga tagttgctaa ttcgacattg gcaacggtca aaagaagaga
    61681 aaacaacaat ccaaatgtaa aaagaatgta ttggatatag ttgaacgctt ctcttaacga
    61741 agcgattttt tcaactaata aaagagaaat caaagagata ccatatattc cgctgaacaa
    61801 gaaaacgtac atctggaaca aataaagaaa ttgctcctta taatacataa aaataaaacc
    61861 accaatgatt cctgcaatta tgaatgtaat aatgattttc tttttcccca ataaacacca
    61921 caatacttga tgtaatgaaa agtcattatt caacaataaa ctgatttctt tattcatcaa
    61981 taagaaaaag gatgataaca aaaggaataa aagaatcaat ccaatagtta aatacactcg
    62041 ataattatat acgtctgtat ttgaaataac cgaatacgaa tagctatttt caaagtcttc
    62101 aaaaatatta gtggcataat cagtgtgaaa tgcttgattg aatcgatcag tgaatagtga
    62161 aatgacccgt tcataggctt ctttttctct cgtcatcaag aaaaattcac cttcaaaatc
    62221 ttttattttg atcgattctt caatggacgt tacgcgaaag gaggtattag aatagggtac
    62281 cttctcgtga gaagatttga catccccttg ataatacaat gtcatttcaa gctttggctg
    62341 tatcttgaag ttccaagatg tatctacttc attggattca tttactgttt ttttcatata
    62401 agaaagctca gtttctgatg agacattttc aaataccatg attagctgct catattcttc
    62461 ttgcccaata aaatgttgtg gcaatcgaat cggagtataa gtatctgttt gatggaaaaa
    62521 ttcatttgtc cgatagagta cctgtttatt tatactaacc aaggcaaaac cactcatgat
    62581 accggtcact atgattgtga gtaaaactgt gaactttttt ttgattatca ccactctccc
    62641 ttacttaaat gattctttga taaaatcata ccgtttttca tcaaaaaaac gtatctatca
    62701 agtgtatagg tcatgtagaa taaatctata tactgtagaa tcgaaaggag aagaagatga
    62761 acaccggaga aaaaatacga acttaccgca gaaattgtaa gcttacgcaa aaagaattag
    62821 gcgaaaaaat tggtgtttcc gataaaacga tttctagttg ggaaaattct cgtacaatgc
    62881 cagactttga aatgctgtca cttttacatc atactttagg tctgccgatt gattttcctt
    62941 ctattgaatc agctaccgaa tcaatagaac caacattgcc agatgaacct cttgttaaca
    63001 agaaatctta ttcatcaaaa caacccttct cactcaaaaa gacactatct ttctttctgc
    63061 tcgtcttttg tttggtctat ctttttctta tcaattatca atacaaaaat gaaacgatcg
    63121 atcgattcaa tccttttttg aaaccacaaa tcggctatac cacactccct gtaggatacg
    63181 tggacaattc catcgaacgt tgggtatgta atgatatctt tggccatggt acatggctgt
    63241 cttttcacgc tggagaggta gaaaaagaga aaaaccatgc tgtaatcaaa cacaaaggaa
    63301 aacacgtcat gaacgttcgc ctgctcactt gggaagccat tccctcagat taccaagaaa
    63361 tcctcagcca agactatgac gacgccttaa aaagaagccc tcaggagcca gataggcatt
    63421 aagtaggaga tgaacgtaga gatagaaaaa atcaatgcac atgatcttat ccaaaaaaca
    63481 ggggtacaaa ttaattgaaa aagccttgag atgaaagttc ctaaataaaa aagttcagaa
    63541 tgataggtat ttctggctta tagtataata tttacgccac agccttccaa tgattctttt
    63601 tggaatcata atcaaggatg gacattcaca aaaatatttt gctcattcga tccgaataga
    63661 ttaacgacca atctagtaag taatatagat tggtacgtag tcaagctgca cataatttag
    63721 atcaatttta gttctttttt atatttctat gatctgctca tttactttta tatgatggct
    63781 tacagtaaca taaataaata tgataatcgc tggtagttta tccaagagta ctcaataaaa
    63841 tacaaaatgg attgttcaag cgcctatcat aatgataaaa tgaaacaaat aaacgataca
    63901 aggaggaaga gactttgagt aatggcagaa caattttttc attgtaaaaa ttgttcagtt
    63961 gttaatctat gggatttcgg tagcatgggc atttttttat agaggaatca caccaacctg
    64021 gccgttttat ctcttgttag tgggaatatt agtaggaaat tttgcccctc ttcactatcg
    64081 tacgaacttt tttccaactg attctggcct ttttcttaaa acaaagacgc gaaaattaga
    64141 aaacattatt gaaatgacaa ttacctgtat aatcattttc ttagttatat tcctaaacgt
    64201 cttctaataa gaatctctta aaattagctt gttacagtta gttacctagt ttatataaca
    64261 cagttatctt atcttcctta tcaacatctt ttgccaactc tttgtcttaa attcgaaaca
    64321 tataagcaga gctacgaaca ccaagactgt ttcggctctg ttttttgcat caaattcact
    64381 cttatatagc tgataatctc ggctctttaa aagaaattat tttcgcttca ttttattata
    64441 aaaaggctag gaaccttgat gttatcaggt tcctagcctt tatttaagta ttaccccaca
    64501 gacccttcca tatagatttt caaattacac actagtttaa gataaaacaa acaaggaaaa
    64561 acttaccatt tactcaaaaa attaactcat ataagtacat tagaatgtaa aatagactgg
    64621 tcaaaatctg gtcaaaaaag tagatgtgac acaagttcgc attcaaagcg atgatccatg
    64681 atttcgtaat ggtcaattat atggatcaaa gccacgcgac aagagcgcaa tggatgtctc
    64741 taccgattgg ttgaataaac gaaataaata gaatgtgaaa aaggatcgga caaatgttcc
    64801 agtttgctcg atcctttttt atttgaattc attttcgctt tatcaaacat catttgatta
    64861 ctcagttaaa tacttttgac atgagtacca atgaaacatt tattcattaa aattacttat
    64921 ttcttatcgc ctttctagcc aatgccaatc cgccgattat tccagcacta tcacctaatt
    64981 cgggcgtaac aatgtagcta gcaattggtg gtatttcaac atatccattt ataagctcaa
    65041 caaatttatc ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa
    65101 taattctatc tggcgaaagc atcagtgttg tattgtatgc acattgggca aggtaaaagg
    65161 cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaatcgtt
    65221 tttcaatcgc tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc
    65281 ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga
    65341 acccttccac gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga
    65401 aatatgcaac acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg
    65461 cattaacatc tgttgtccat tcaatcggaa tattgaaatg ttgctttagc gttcctacaa
    65521 aattgaaatt ctgccaagca agtttcggtg ttgatgtgat atgaccgtat ttctttgatt
    65581 tacgttgaat atcaattgga ccaaaacagc cgaccccgat tcctgccaat tgttccttaa
    65641 aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac tggagtcgtt gtgggaaaac
    65701 tgactctttc caaaatcgtc aattcctcag tactgactgc acaaataaat ttagtccctc
    65761 cagcttcaat tgccccatat atcatcatag gcttctccct tcgaatatgt gtaaaacact
    65821 tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc
    65881 tgttgaatac caata
//
LOCUS       DACIWG010000009        65434 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.009, whole genome shotgun sequence.
ACCESSION   DACIWG010000009 DACIWG010000000
VERSION     DACIWG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.009"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IYF54_000560"
     CDS             complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IYF54_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ9830919.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2146..3117
                     /locus_tag="IYF54_000561"
     CDS             2146..3117
                     /locus_tag="IYF54_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ9830920.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3141..5333
                     /locus_tag="IYF54_000562"
     CDS             3141..5333
                     /locus_tag="IYF54_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ9830921.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5350..5826
                     /gene="ybeY"
                     /locus_tag="IYF54_000563"
     CDS             5350..5826
                     /gene="ybeY"
                     /locus_tag="IYF54_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ9830922.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5804..6205
                     /locus_tag="IYF54_000564"
     CDS             5804..6205
                     /locus_tag="IYF54_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ9830923.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6220..7119
                     /locus_tag="IYF54_000565"
     CDS             6220..7119
                     /locus_tag="IYF54_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ9830924.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7262..8074
                     /gene="recO"
                     /locus_tag="IYF54_000566"
     CDS             7262..8074
                     /gene="recO"
                     /locus_tag="IYF54_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ9830925.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8458..9375
                     /gene="glyQ"
                     /locus_tag="IYF54_000567"
     CDS             8458..9375
                     /gene="glyQ"
                     /locus_tag="IYF54_000567"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ9830926.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9377..11452
                     /locus_tag="IYF54_000568"
     CDS             9377..11452
                     /locus_tag="IYF54_000568"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ9830927.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11836..12615
                     /locus_tag="IYF54_000569"
     CDS             11836..12615
                     /locus_tag="IYF54_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830928.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12656..13600
                     /locus_tag="IYF54_000570"
     CDS             12656..13600
                     /locus_tag="IYF54_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9830929.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13616..14395
                     /locus_tag="IYF54_000571"
     CDS             13616..14395
                     /locus_tag="IYF54_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ9830930.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14408..15106
                     /locus_tag="IYF54_000572"
     CDS             14408..15106
                     /locus_tag="IYF54_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ9830931.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15134..15898
                     /locus_tag="IYF54_000573"
     CDS             15134..15898
                     /locus_tag="IYF54_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ9830932.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15911..17728
                     /locus_tag="IYF54_000574"
     CDS             15911..17728
                     /locus_tag="IYF54_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9830933.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17742..18965
                     /locus_tag="IYF54_000575"
     CDS             17742..18965
                     /locus_tag="IYF54_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAQ9830934.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18979..19713
                     /locus_tag="IYF54_000576"
     CDS             18979..19713
                     /locus_tag="IYF54_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ9830935.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            19730..20341
                     /locus_tag="IYF54_000577"
     CDS             19730..20341
                     /locus_tag="IYF54_000577"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9830936.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
     gene            20344..21489
                     /locus_tag="IYF54_000578"
     CDS             20344..21489
                     /locus_tag="IYF54_000578"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9830937.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
     gene            21512..22564
                     /locus_tag="IYF54_000579"
     CDS             21512..22564
                     /locus_tag="IYF54_000579"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9830938.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
     gene            23093..23869
                     /locus_tag="IYF54_000580"
     CDS             23093..23869
                     /locus_tag="IYF54_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ9830939.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            23880..24341
                     /locus_tag="IYF54_000581"
     CDS             23880..24341
                     /locus_tag="IYF54_000581"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ9830940.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            24400..25857
                     /locus_tag="IYF54_000582"
     CDS             24400..25857
                     /locus_tag="IYF54_000582"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAQ9830941.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            25892..27061
                     /locus_tag="IYF54_000583"
     CDS             25892..27061
                     /locus_tag="IYF54_000583"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9830942.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            27099..28190
                     /locus_tag="IYF54_000584"
     CDS             27099..28190
                     /locus_tag="IYF54_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9830943.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(28258..29736)
                     /locus_tag="IYF54_000585"
     CDS             complement(28258..29736)
                     /locus_tag="IYF54_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ9830944.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(30002..30750)
                     /locus_tag="IYF54_000586"
                     /pseudo
     CDS             complement(30002..30750)
                     /locus_tag="IYF54_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            30893..31843
                     /gene="pfkB"
                     /locus_tag="IYF54_000587"
     CDS             30893..31843
                     /gene="pfkB"
                     /locus_tag="IYF54_000587"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9830945.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31825..33288
                     /locus_tag="IYF54_000588"
     CDS             31825..33288
                     /locus_tag="IYF54_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9830946.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            33285..33743
                     /locus_tag="IYF54_000589"
     CDS             33285..33743
                     /locus_tag="IYF54_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9830947.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            33740..34714
                     /gene="lacD"
                     /locus_tag="IYF54_000590"
     CDS             33740..34714
                     /gene="lacD"
                     /locus_tag="IYF54_000590"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9830948.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(34893..35843)
                     /gene="yidC"
                     /locus_tag="IYF54_000591"
     CDS             complement(34893..35843)
                     /gene="yidC"
                     /locus_tag="IYF54_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ9830949.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYHENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(36281..36556)
                     /locus_tag="IYF54_000592"
     CDS             complement(36281..36556)
                     /locus_tag="IYF54_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ9830950.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            36664..37428
                     /locus_tag="IYF54_000593"
     CDS             36664..37428
                     /locus_tag="IYF54_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ9830951.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            37551..38054
                     /locus_tag="IYF54_000594"
     CDS             37551..38054
                     /locus_tag="IYF54_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9830952.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            38462..39109
                     /locus_tag="IYF54_000595"
     CDS             38462..39109
                     /locus_tag="IYF54_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ9830953.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            39272..40540
                     /locus_tag="IYF54_000596"
     CDS             39272..40540
                     /locus_tag="IYF54_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ9830954.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(40567..41766)
                     /locus_tag="IYF54_000597"
     CDS             complement(40567..41766)
                     /locus_tag="IYF54_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ9830955.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(41885..43192)
                     /locus_tag="IYF54_000598"
     CDS             complement(41885..43192)
                     /locus_tag="IYF54_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ9830956.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            43663..44781
                     /locus_tag="IYF54_000599"
     CDS             43663..44781
                     /locus_tag="IYF54_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830957.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            45165..45485
                     /locus_tag="IYF54_000600"
     CDS             45165..45485
                     /locus_tag="IYF54_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830958.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            45937..46137
                     /locus_tag="IYF54_000601"
     CDS             45937..46137
                     /locus_tag="IYF54_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9830959.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            46284..49073
                     /locus_tag="IYF54_000602"
     CDS             46284..49073
                     /locus_tag="IYF54_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9830960.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEMELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            49120..49647
                     /locus_tag="IYF54_000603"
     CDS             49120..49647
                     /locus_tag="IYF54_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9830961.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            49668..50858
                     /locus_tag="IYF54_000604"
     CDS             49668..50858
                     /locus_tag="IYF54_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9830962.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            50898..52196
                     /gene="asnS"
                     /locus_tag="IYF54_000605"
     CDS             50898..52196
                     /gene="asnS"
                     /locus_tag="IYF54_000605"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ9830963.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            53373..54047
                     /locus_tag="IYF54_000606"
     CDS             53373..54047
                     /locus_tag="IYF54_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9830964.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            54044..54385
                     /locus_tag="IYF54_000607"
     CDS             54044..54385
                     /locus_tag="IYF54_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830965.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            54415..54774
                     /locus_tag="IYF54_000608"
     CDS             54415..54774
                     /locus_tag="IYF54_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ9830966.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            54977..55156
                     /locus_tag="IYF54_000609"
                     /pseudo
     CDS             54977..55156
                     /locus_tag="IYF54_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            55298..57379
                     /gene="cadA"
                     /locus_tag="IYF54_000610"
     CDS             55298..57379
                     /gene="cadA"
                     /locus_tag="IYF54_000610"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ9830967.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            57689..59155
                     /locus_tag="IYF54_000611"
     CDS             57689..59155
                     /locus_tag="IYF54_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9830968.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            59770..60309
                     /locus_tag="IYF54_000612"
     CDS             59770..60309
                     /locus_tag="IYF54_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ9830969.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            60383..60823
                     /locus_tag="IYF54_000613"
     CDS             60383..60823
                     /locus_tag="IYF54_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ9830970.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            60836..61045
                     /gene="copZ"
                     /locus_tag="IYF54_000614"
     CDS             60836..61045
                     /gene="copZ"
                     /locus_tag="IYF54_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ9830971.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            61045..63231
                     /locus_tag="IYF54_000615"
     CDS             61045..63231
                     /locus_tag="IYF54_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ9830972.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            63251..65422
                     /gene="copB"
                     /locus_tag="IYF54_000616"
     CDS             63251..65422
                     /gene="copB"
                     /locus_tag="IYF54_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ9830973.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT        21806 a        11006 c        13584 g        19038 t
ORIGIN      
        1 tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg
       61 ttattcttct ggtaactcac ttgcttcttg tttatatcct gatacgcgat atacataata
      121 aaagagcaag ctgccagcgt aaccaagcaa tgttgggacg acccagccaa gacctaatgc
      181 agcaaaagga acataatgat gataaaatcc tagtaagctt gctacgacag tttgctgact
      241 cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat
      301 cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagtaagat
      361 taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt
      421 atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat
      481 ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat
      541 agcagtagtg aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc
      601 taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt
      661 attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac
      721 gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc
      781 acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt
      841 gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg
      901 gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag
      961 aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag
     1021 gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt
     1081 ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc
     1141 aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc
     1201 gacaggttta gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc
     1261 taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga
     1321 tccagccatt tgacctaagt gtacaggaaa gatcagatta cctgacccga agaacatccc
     1381 gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat
     1441 aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa
     1501 ataccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt
     1561 ccacttggga aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc
     1621 ttcttcattc acggacgctt ttcgataaag ttatccgcta gcctgataat ggaggcagcc
     1681 acaatgatct acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt
     1741 catttctcac ttacatgact cactgggaat aaatcactga atagcctact gttttcctcg
     1801 acgctttgct gtatatttat ttgttgacgt tataatacac ctgttttttt tgaaatgcaa
     1861 gaatatttca aatattttgt tttctttttt tgaaacaaga aaaataccca gagaatactt
     1921 gctaagttag acggaatact tgtcctctat cttatgcata gcttacatga tcagcttttt
     1981 ttattcaaac gagaccttta gagaatggaa gcggctagtt ttttaacgtt tcttggtact
     2041 tatggttaag caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat
     2101 ggtaaaatag gaaagaatta atcgaaaaaa aggggatgtc tatttttgac ggaagagtct
     2161 agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac
     2221 gacaagcata taaaactgat tgaggaaaca acggaaacaa ccatccatac acgtggagaa
     2281 atcattcaga tcattggtga gcaatcccag atttccttgg ctgcctcagt cattcggaca
     2341 ttacaggaat tgatcaaacg aggaatccac gtaagtacgc ccgatgttgt gacggcattg
     2401 aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc
     2461 aaagaccgta atggaaaacc tatacgggta aaaaacagcg gccaaaagaa atatgtcgaa
     2521 tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt
     2581 cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg
     2641 acgcggcctg ctgtggaagc aggtgaaaat ctaggctttc ttcccgggga tctaaaagaa
     2701 aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat
     2761 catacgaacc gattgatgga acgaggcgta atcgaaatcg cacctttagc ttacatgcgg
     2821 gggcgaacat tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct
     2881 cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat
     2941 acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca
     3001 ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac
     3061 cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct
     3121 ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg
     3181 gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat
     3241 ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta
     3301 tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag
     3361 cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc
     3421 ggatcaagca actatttgaa ttgatcgata agacgaatga tgccatcaac aaatcttatg
     3481 acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc
     3541 ggatcgccgc gctgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gtgttttatc
     3601 aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact gagattcata
     3661 ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa
     3721 gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac
     3781 cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat
     3841 tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga
     3901 tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga
     3961 aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca
     4021 tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa
     4081 atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag
     4141 tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt
     4201 atcccgctgc ttttgcacca cttgttttaa gctttttctt gaatcgaaga gcagggatca
     4261 tggctggtat tttccaagca gtatccgcac ttttcatttt ttatgattcg attggcacga
     4321 actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca
     4381 gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg
     4441 tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt
     4501 ggttaatgct cgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc
     4561 tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca
     4621 gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc
     4681 atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt
     4741 tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt
     4801 tcgttgaaaa cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca
     4861 gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa gattataata
     4921 tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt
     4981 tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg
     5041 gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg
     5101 cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta
     5161 tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc
     5221 gaaaagtgga aaaatcatta atcagcggtt taagtagcac cttccattct cggatcaaat
     5281 atcctaaaat gaaaacagaa gttgagaaga taaaggaagt acaagaagaa tagaaagaag
     5341 cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg
     5401 aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca
     5461 gaaatgtctg ttactttcat ggacaatgcg gcaattcaag taatcaatcg tgattaccgt
     5521 ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa
     5581 atcccagtga tttttgaaga tggagagaat ccattacctc gtgtactagg agacatcatg
     5641 atctctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa
     5701 cttggctttt tagcggtaca tggatttttg catatcaacg ggtatgacca tatgacacca
     5761 gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa
     5821 agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg
     5881 agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct
     5941 tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt
     6001 agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga
     6061 catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg
     6121 agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa
     6181 aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg
     6241 tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg
     6301 ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga
     6361 gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca
     6421 aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat
     6481 gcagtcttat ttatgatcag tgctgatcaa aaacgtggcc ggggagacga ttttattatt
     6541 gaacgtctaa aaaacgtgca ttctcctgtt tatctgatta tcaataaaat cgataagatc
     6601 catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa
     6661 gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc
     6721 agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa
     6781 tacttcatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg
     6841 cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat
     6901 atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc
     6961 ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga
     7021 gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc
     7081 tatctgcaag acttcggcta ccgaaaagac gagtactgat attctggact tcttataggt
     7141 taggtataga aataaactgg ataaaaatcc gtacgttgag aaaaacagcg gaagatcgaa
     7201 acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa
     7261 tatgagccag cttgtagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa
     7321 agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg
     7381 aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta
     7441 tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc
     7501 gtttcggaac atccaagaag atatcttttt gagcggttat ggtacgtatt tgctaaatct
     7561 agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca
     7621 agctctgcag cggatggacc aaggagatga tgcagaaatc atcacgaata tctttgaagt
     7681 gcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga
     7741 aacaaaagga aaatttgact attcttccaa atatggcggt gtgatatgcg agaaacactg
     7801 gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt
     7861 ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc
     7921 aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa
     7981 aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat
     8041 gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa
     8101 actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc
     8161 actgagagga tctattatac aaaccagaaa actgcaaaat gaatacgtac attgaccaag
     8221 atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt
     8281 tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg
     8341 gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt
     8401 tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg
     8461 agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac
     8521 ggctgcatgc tgatgcaagc ttatgataca gaaaaaggag caggtacgat gagcccatat
     8581 acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc
     8641 cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt
     8701 caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt
     8761 cttttaggaa ttgatccatt agaacacgat attcgctttg ttgaagacaa ctgggagaac
     8821 ccatcgatgg gttgtgccgg tgtgggctgg gaagtatggc ttgatgggat ggagatcaca
     8881 caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc
     8941 acttatggac tagaacgtct tgcttcttat atccaagaag ttgaaagtgt ttatgatctg
     9001 gaatgggctg atggcgtgaa atatggtgag atcttcaaac aaccagaata tgaacattct
     9061 aaatattctt ttgaagtaag cgaccaagac ctgctactaa gcaattttga tcgttttgaa
     9121 aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg
     9181 aaatgtagcc atacattcaa tttattagat gctcgcggtg cagtatccgt aacagagcgt
     9241 gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca
     9301 gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacgtg
     9361 aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc
     9421 tgctcatgta gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga
     9481 tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat
     9541 caaagtaaca aacattccag aacgtcaagc agacagcgaa gaagaagtca aaggaccagc
     9601 caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg
     9661 cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt
     9721 ctatgtaacg aaatacactc acggcaaaga agcaaaagaa gtattgacag gtttagcaga
     9781 tgtcattaaa agcctcactt tcccagtaac gatgcactgg gcagaatacg attttgaata
     9841 tattcgccct attcactgga tcgtggcatt gttagatgac gaaataatcc cgtttgagat
     9901 cttggatgtc aaaacagaca gagtttcaag aggtcatcgt ttcttaggcg aagatgtgac
     9961 atttacgcat gcaaaagaat acgaagaaaa attgaatgaa caatttgtta ttgcttcacc
    10021 gcaaaaaaga aaagaaatga tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg
    10081 gatcattgat ctagatgaag atttactaga agaagtcaac aatctagtcg aatatccaac
    10141 tgtcttttct ggcgacttcg aagaaaaata tctatctgtt cctgaagaag tactagtcac
    10201 ttctatgaaa gaacatcaac gttatttcga agtacggaac gatcaaggta tgttgcttcc
    10261 tcattttatt tctgctcgta atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa
    10321 tcaaaaagta ttgactgctc gtttagaaga tgcggaattc ttttataatg aagacaagaa
    10381 agcttcaatc gaccaatacg ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg
    10441 tagcatctat gagaaaatgc aacgggtaca agtcatcgct cgcatcatag gcgaacaagt
    10501 aggactatct gaggaagaat tgatggatct gcaacgcgct gctgaaatct ataaatttga
    10561 tctagtaacc aacatggtag gagaattccc tgaactacaa gggatcatgg gagaaaaata
    10621 tgctctgctt aaaggcgaga aaccagccgt agcaacagcg atccgtgaac attacatgcc
    10681 tatatccagt gaaggagatt tacctgagac tacagtaggt tctgttttag cgattgctga
    10741 taaattagat agtatctttt ctttctttgc agttgggatg atcccaagtg gctcaaacga
    10801 tccatatgct ttgcgccgtc aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg
    10861 ggcctttcct ttgtctgttc tgcaagaaac gattagtgaa gttatcagta aagatacgga
    10921 tcgttttggc attggcttgt cagcgggaca acaacaggtg atcgacttta tcaaaggccg
    10981 tctgcgccaa ttattgacaa cgaagaatat ccgtcatgat gtcatcgaag ctgttttgaa
    11041 tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca aacaacatct
    11101 tgcagatgaa gacttcaaac cttcaatgga agcattgaca cgtgtcgtga acttagcgaa
    11161 aaaagcgcag cttgaaaaac aatcagaagt agaccctgag ctgttcgaaa acgacgcaga
    11221 gaaagaatta cacaaagcag tagaagcaat gaaagcaagt tttgctgacc aaacgatcca
    11281 cgaaaactac gaccagcttg tcgctcttcg tccactgatc gaaaattatt ttgatcaaac
    11341 gatggtcatg gctgatgaac cagcagtacg taagaaccga ctaacacaac ttagtcaaat
    11401 aactgccatg gctttgtcat tagcaagctt ggatcaaatc atcacaaaat agtcttttgt
    11461 ttgaaaaaca gagagcatgc acgaaacgtg ctgtgtctct gtttttttgt tataaatata
    11521 aaagctgtta ttaattctat tttaataatt tttgttattt tattctttgc tagaattttc
    11581 aaaacgctca tgaaaagtga ggaaataaaa atgaaaaaga ttaataataa tcttgttact
    11641 gttttattta cttttaaatt aggaatttcc ctagtcaaga aaactgtttg tggtatgatt
    11701 atatagggaa aacctgttta taaatatttc tatgaatata ttagtttaat tatgctttta
    11761 aaataaaaag cgctcattct gatgtgaatg aatcacatct ttatttatct atcatcaata
    11821 tttaggagtg aatctatgga aaagccgttc gtcaagttat tggctactgt ggcggtaggc
    11881 acaggcgcta ttgtgatttg cttgtttgga taccatctca ataatcagcg gcaacatcat
    11941 caacggatca attacgcaga atctgccatc accaatcaaa aagacaccgt gacctcttta
    12001 tcaaaagaag tagataagct gtacagtaca aaagaaaaaa ttttcctaaa tcctgaaata
    12061 acagaagaaa cagtttcaaa tttgtcacat aaactatctt ctatcaaact ttcagcagat
    12121 gattatgaca tcaaggaatc agaattacct aaagaagctg ctgcgatcca agaagaaaag
    12181 aaagcagtct taacacagtt agaggatgca gaatcaaaac tgaagatcca aacggctgtc
    12241 aataaattat ttactaaaaa cgtatcaaac tggcagcaag ctgtggatga tgtcatcatc
    12301 aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg aaaatatgag cttttttaag
    12361 gatagtgctt ggaaaacggt cgttatgcaa tatctaggat tcgctgatac ccaaattgca
    12421 caagtcacgc aactagatca gctattcgat acgatgctga aagacggtca agtaacagct
    12481 actgctactt acgatcaata tttgaccgca ctcagtctca tcgaacagat ccggaatgaa
    12541 aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg ctcagcaaat gggctatagc
    12601 aatacgagct attaataagc gagaattcaa gcaaggctag caaacggagg aaactatggc
    12661 aaaacgaaga agaagatcaa gagggaaaaa gatatttcta attattttag caattcttgt
    12721 cgtattagta ttagcaatcg taggagtagg agcaaaatta tatctagatc tctcacattc
    12781 tattgaaaaa acttacgaat cagtggaacg aacccaagga gaacgaacaa aaccagttga
    12841 tctggacaaa caagaatctt tctctgttct attgatgggg attgatacgg gagatttagg
    12901 tcgggtcgat caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa
    12961 acagacaacg attgtcagca ttcctcgtga tacgtatgtc gacatcgttg gacatgacac
    13021 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
    13081 acaaaaatat gtagacatcc caatcgatca ctatgtttca atcaatatga aaggtctaaa
    13141 agaactcgtt gatgcagttg gtggtatcga agtcaataat gacctcacct tctcacaaga
    13201 tggctatgat tttacgatcg gtaaaatcac attgaacggg gatcaggcat tgtcttattc
    13261 tcgtatgcgt tatgaagatc caaatggtga ctacggcaga caagaacgtc aaagaaagat
    13321 cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt gtaagcaact atcaatccgt
    13381 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaagat
    13441 tgctttagat tatcgggatg catttggtac gatcaagcaa gatcagctgc aaggagaagg
    13501 cttcatgcaa gacaatatct cataccaaaa agtcagcgat caagaacttg atcgtgtgaa
    13561 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
    13621 agagacaatc agcttgaaag aaatttttga aacattacgc aaacacgtga taacaatcct
    13681 catctcaatg tttgctggct tggccatctc aggcatcgtg acgtttttca tcttgacgcc
    13741 aaaatacagc tcacaagccc aattgatcgt gacactgccg caatcagata ctgcaaatgt
    13801 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaggatatga tcgtcagtga
    13861 tctagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtaa aaatgactgc
    13921 tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
    13981 ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc gctaatacaa cgtcacaagt
    14041 attccaagag aacgccaaag acgtgatgaa tgtcgataaa atctcgatca tctcaggtgc
    14101 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
    14161 gttaggaatg atggtaggcg taggtctagc tttcttattt gaattactcg accggacagt
    14221 aaaagatgaa aaatatgtga cggatacatt gggcttcccg atcttaggga ctgtaccaga
    14281 aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa ggaccagctc cgacacaacc
    14341 aaaagctaca aacgataaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga
    14401 aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
    14461 cacactcaca aacccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
    14521 ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt gttgtgactt catcaggacc
    14581 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
    14641 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
    14701 gaacaacgca agcggattaa gtaccgttct tagttcatca ggaagtgtag cagatgagat
    14761 ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt ggacctaaac caccaaatcc
    14821 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
    14881 tgatgtagtg atcttcgaca tgccgccagt agttgccgta acagatgcac agatcatgtc
    14941 ttccaaaacg gatggcacct tattggtcgt acgtgaaaat atgtcacgga aagaatcatt
    15001 gactaaagca aaagaattgc tggatatggt acaagcgcga gtccttggtg ttgtatacaa
    15061 tggggcagaa cacagcaaag atgctggcta ttactacggg aattaagaaa taaaaagaga
    15121 ggagacaaga gaaatgatcg atctacattg ccatatcctg ccaggaatcg atgacggagc
    15181 agaaaacata gatgccagta tcgccatggc tgaaaaagcg attcaacaag ggatcacaca
    15241 tattttatgc acaccccatc ataataatgg aaaatacagc aatgaaaaat cacaagtcat
    15301 ttctctagtc gcttcgttac aagcagaatt agagaaaaga caattgcctt tgacgttatt
    15361 ggaaggacaa gaagtacgta ttaccggaac actgatcgaa gacatcgacc gagatgaaat
    15421 tttatttaca gatttagatg atacatatct tctaatcgaa tttccgacac tggaagtgcc
    15481 gttatatgca gaacagctct tttttgagtt gttacaacta ggaaagacac cagtgatcgt
    15541 ccatccagaa cgcaacgcgc attttcgtaa agacccgaat catatgctgc cattcctaga
    15601 tatgggatgc ttggcccaac tcaccgcacc aagttatgtc ggtgtatttg gaaaagacat
    15661 ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg cagatggctg cttctgatgc
    15721 ccatggggtc tctaagcgca ccttttactt aaaagagtgc tataagcaga ttgccaagga
    15781 ctttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
    15841 tgaaatcagt tatccagtct atgaagagat aaaaaagaag aagtttggtt tattttaggg
    15901 ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt gccacattgg ttgttgtgga
    15961 cagtttgtta ttgatcgctg caaatattgc agcagctaaa ttcatgaagc cgtttgtagc
    16021 tataccaatg gaccttattt taatctctac cggtttgtcg attggttttt atttatttta
    16081 tggtagttta ttcaaagttt tcactcggat caatcgctat acaaatttaa gagagatcat
    16141 tgcaattttt ggttcactat ctgcaagtga cgtctcaagt atcctcatac tgctgttcat
    16201 taatcggcaa tacagtttac ggttagttat ctttgcttat ctgctttcgc tcttattgat
    16261 catcggtagc cgattgatct ggcgtatcta catcgaaaca aaaaatatga gatatgtatc
    16321 agcggattct gctaaaaata cattgatcgt tggtgctggc gaaggcgggc gtattttata
    16381 caacagcttt ctgggatcca aaacggctca agatatccaa gttgtcggat ttgtagatga
    16441 tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga
    16501 tattccggaa ttgattgaaa aatatgacat tcaaatggta acgatcgcta tcccttcctt
    16561 atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa tccgctcacg tgaaagtcaa
    16621 cacgatgcct tctatagaag aacttgcttc aggaaaaatt agcgtatcaa aattaaaaac
    16681 aattgacgta gtggatttac tgggaagaga tgaggtaaag ctggatattg aatccatcaa
    16741 agatcagatc acagacaaag tcattcttgt caccggcgca ggaggatcga tcggttcaga
    16801 aatctgtcgg caaatcattc aattcaatcc tgcaaagcta ttgcttttag gacatgggga
    16861 gaactcgatc tacttgattg accgtgagtt acgcacgcat catcaaaatt gtcctacaga
    16921 aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa atcaatgaga tcatggaaca
    16981 atatcatcca gatatcgtct accatgcagc tgctcataaa catgtaccac tgatggaata
    17041 taatccaaaa gaagcagtca aaaacaatat tttcggtaca aaaaatgtag cagaagcagc
    17101 taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca gataaagcta acaatcctcc
    17161 aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc gtgactggat tgaatgaaga
    17221 aggctgtacg aaattctcag ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt
    17281 gattccagta ttcagagaac aaattgctaa aggtggtcca atcactgtaa cagactttcg
    17341 tatgacccga tacttcatga cgattcctga agctagtcgt ttagttattc aatctggcgc
    17401 attagcaaaa ggtggcgaaa tttttattct agatatgagt gaaccagtga agattgtaga
    17461 cctagccaaa aacatgatcc gattgagcgg gtatagcgaa gacgaaatcg aaatcattga
    17521 aacaggtatc cgtccagggg aaaaactata tgaagaacta ctgttggata aggaaagaaa
    17581 tgatgaagca gtttatgaga aaatctttgt aggaaacatc aaaggttact caatacaaaa
    17641 agtgatggat tttgtcaaga gcctgccgca agacgatgaa caattggcta aagatatcgt
    17701 cacttttgca aatgcttcga ataaataggg gggaaatata aatgaataaa ttagaaaaac
    17761 gtcagatacc attttcaccg ccagatatta ctgatttaga aataaaaaac gttgtagaag
    17821 tattaaaatc tggatggata actacgggac caaagacaaa aaaattagaa aaaaaaatta
    17881 caaagtggtt aggaacttct aaagtagttt gtttaaattc acaaactgct gctgcagaaa
    17941 cagggttacg tttgctggga ataggagaaa atgatgaggt tatcgtccca gcttatactt
    18001 attctgccag tgcatcggtt gtatgccaca taggcgcgaa cctagtatta atagactgtc
    18061 aacaagattc tttggagatg gattacgatg cattagaagc agcaattacg gagaagacaa
    18121 aagtgattat tcctgttgat cttggtggta taccttgtga ctacgaccgt atttttgaga
    18181 ttgtggagaa aaaaagaaat ttatttcaac ctagtaatga gattcagaaa gcttatggtc
    18241 gtataattgt tatggcagat tctgctcatg catttggagc taattggcat gatcagatgg
    18301 ttggtaatgt tgctgacttg acaacatttt catttcatgc tgttaagaat tttacaactg
    18361 cagaaggtgg agctttagta tggaaagagc acgacaaact agataatgag gcaatgtatc
    18421 atcagtgcca gctgctttct ttgcatggac agtcaaaaga tgcacttact aaaacacagt
    18481 tgggtgcatg ggaatatgat attgtgggtc catggtataa atgtaatatg actgatgtta
    18541 ctgcatcaat tgggttggca caatttgaac gctatcctaa tattctgaaa agaagaaaga
    18601 atataattaa gaggtatgat gaagcctttc aacaactagg aattgaagtt cttatgcatt
    18661 atacggatga ttatagttct tcaggccatt tatatattac tagagtacct aacattagtt
    18721 ctgagcaaag aaatgaaata attataaaaa tggcagaaca agggattgct tgcaacgttc
    18781 actataaacc acttccaatg tttacagcat ataaaaatat ggggtttgat attaataact
    18841 atcctaatgc ctatgctcat tttgtaaatg agattacatt accattgcac acgaaactta
    18901 cagatgaaga agtagaatac gtaattaata actatagtaa gattgtaaag gatttggttg
    18961 gatgatagga gaggcaaaat gagaaagtgg gaagatttgc caaaagaaat gcagaatgat
    19021 gaaatacggt attattatga tatcctcagt aaaaaaaaat tcagcttagt ggtaaaaaga
    19081 gtatttgata ttttagtgtc tgcaactttg ttactgacac tttcatggtt attttttatt
    19141 cttgcaatag tgataaaaat tgattctcct ggacctgtat tttatagaca agaacgtatt
    19201 actcgttatg gaaaaaaatt taaaatttat aagtttcgta ctatggtaat aaatgcagat
    19261 aaagatggca ctcttgttac tgtgaaaaat gatgatcgta ttacaagagt aggaaaggta
    19321 atccgtaaat atagattaga tgaaattagt cagctaatta acgttttggt cggagatatg
    19381 tcttttgtag gaaccagacc agaagttgga aaatatgtaa agtcgtattc accattaatg
    19441 ttagccacat tattattacc agccgggatt acatcagaaa ctagtatttt atacaaagat
    19501 gaagataaat tgttgcatga taaaaataac atcgacaaaa cttatataga aaaaattctt
    19561 cccagtaaaa tgtattacaa tttgaaaagt atcagtgaat ttagttgtat gaatgatttg
    19621 aaaataatgt gtaaaactat ttttgttata tttggtaaag aatatcacgc cgacttagaa
    19681 ggtgaacgag agtttattaa tcagataaaa tgaatggagg ataagagttt tggtgattga
    19741 agagataaaa agttcggata aagagttaat taattcgatt gtcgatattc atatgagaac
    19801 atttacaaat ttttttttga ctttcatggg aaaaaagttt ttggaattga tgtatcgttc
    19861 ttacacagaa tataatgatt ctggtatatt ggttgctatt caaaatgata agccaatagg
    19921 atttcttgca tattctggag atatgagtgg tttatataag tatatgataa agaaaagaat
    19981 aataccgttt ggattttata gttgtgtagc ctttataaag aaacccaata tccttatgag
    20041 gctttttaga gcttttctaa aaccaagcga aacaaaacgt gatgaaaatt atgtggaatt
    20101 agcatccatt ggagttaatc cgaaagagaa atcgatagga gtaggttcca tgcttataga
    20161 taacttaaag agtagaattg atttttcaaa atatgaatat attgcactag aaacagatgc
    20221 attaaataat gatgttgtga ataattttta taaaaagaat gagttcattt tagaacgtaa
    20281 atattctact aatgaaggaa gaattatgaa tgaatatcgg ttttatatgg agaagaatta
    20341 attatgaaat tattgtatat tttaaatgta gctaataaag taaataactt ttctaaagct
    20401 agtatgatgg cagctaaaca aacgggttta gaattttata tagctggtaa ctggaaatac
    20461 aaatccgatg aggaaagaaa agctgatgaa gtgaaatatg gaataaaaat ataccaagtt
    20521 gatttttata gaaacccact aaatttaaaa aatataaaag cttatcatca gattgtagag
    20581 attataaaaa atgaaaaaat agatattatc cattgtaata cacctattgg aggtgttgtc
    20641 ggacggattg ctggtcttaa atgtaaagtc aaaaaagtaa tttaccaggc acatggattt
    20701 catttttatt caggtgctcc taaaataaat tggtttatat attatcctat ggaacgttgg
    20761 ctagctcatt atacagatgt tttgattaca ataaatcatg aggattatac tttagctcaa
    20821 aaacttcgtc ttaggaaagg tggaaaagct tattatattc caggtgtggg tatagataca
    20881 aataggttta tgtcttccac atcaataagg aattttagag aaagtatatg taaagaactt
    20941 aatatacctg atacagcaaa aatcatttta tctgtaggag agctaaatga gaataaaaac
    21001 catttatcag taatacatgc attacaaaaa attccgctgc atacagatat ctattatatt
    21061 atcgctgggg taggagaaat gagggagaca ctacaaaata ttgctaatga attaggatta
    21121 catcagcgag tttttttact aggatataga agtgatatac ccaaattatt agctgctgca
    21181 aacatatata ttctaccctc attaagggaa ggcctcaacg cctctttaat ggaagcgatg
    21241 gctagtggtt taccatgtat tgtcggtaat ataagaggaa atgtagatct tatagaagat
    21301 ggtaagggtg gatttctaat caatcctaaa gacgaagaag aaattgctgg tgcgataaat
    21361 agattggctg ataacaaatt attgtgtgaa tctatggggg cacataattt agacaagata
    21421 aaagattttg atttatcagt tgtgataaac caaataaata atgtttacca tgaaattgta
    21481 agtaaataat tgaattgaaa gtgatattaa tatgaaaaaa ataaaaatta tgcatttgtt
    21541 gcaaagtaat agattttctg gcgctgaaaa tattgtttgt caaataatga gtttatttca
    21601 aaataacgaa aaatttgaga tgatttacgt tagcccagat ggtcctatag caaatatttt
    21661 aaatattaga ggtttaagtt acttaccatt aaaaaaattt tcgagaaaag aaattgacag
    21721 agccgtacat gaattaaacc cagatataat tcatgctcat gattttaatg ctagtgtaag
    21781 agcaagtaga tataagaagc aaatcatcct ttcccatttg cacaataatc ccttgtggtt
    21841 acctaaacta gacataagaa ctattatata ttctttatgt tcgtctaggt ttaattcagt
    21901 aattggtgtt tcgaattccg ttaaggaaga atttcgttac aaacaattaa taaaagataa
    21961 atttgtgttg ttacctaaca ctgtaaatac tgaaagaatt ataaaaatgg ggaatgaatt
    22021 tgttgaagat gatattgata ttttatatgt aggacgcatg gcagaaccta aaaatccaat
    22081 aggtgcaatt aatgttttag aaaatgtagt aaaaaaatat tcaaaacatt tacgtgtcgt
    22141 gatgattggt gaaggaccat tatttgatga agttaagatg caaatacaaa aaagaaactt
    22201 aacagatgtt attgagttaa agggattcaa aagtaatccc tataagtaca tgaagaaatc
    22261 taaagttcta ttaatgccat ctctttggga agggtttggc ctagtatcag tagaggcaat
    22321 gttgttagag acgcctgttg tagcttataa cgtaggagga ttgaaggata ttatcgatga
    22381 gacaaatgga tttctgtgtg gtagtgaagt tgaattatct aattcactaa tgaagctatt
    22441 aaattcagaa gaacttagaa aaaaaatggg agaaaatgct gctattagtg caaagaaatt
    22501 ttctaatatg aatcgttatc aaggagtatt agaaaaaatt tatgtatctg cagctttaaa
    22561 ataaaatatg aattataaaa atagagatca attagtttta cgaaaaataa tacccattag
    22621 tattttgttt caatcaggaa tgatttttta ttcactgatg ccctcaatag caattgcagt
    22681 tgtttctttt tttatgtttt tgtattatat gtggtgctta aaaaagtatt tttgtttagc
    22741 agatagctta ttgttaacat caattctttt cattccaaca tcaactatat ctatattagg
    22801 aactagttat tctagcttac cattgacgtg gcataatcta ataataataa ttttgatgtt
    22861 atatattatt atagaaggaa cattaaatcg ttcatacttg attattttat ttttatttat
    22921 ttgttttgga atatcgacgt ctatgttctt acctttttta atagatgcat taaagcaatt
    22981 aatgactatt tcgttgttta tctgtagttt tttaatagga aaacgtttta aaatgtatag
    23041 caaaaactta actatgattg ggtggaattt ctatttcata ggagtagtat ctatggcgtt
    23101 tcaggtatat ttgcaacgag tatatatttt atctactggt gtaataatag gccattatgc
    23161 agaaatgggt ttaaatagaa ttgcatatgc aggtttgatg ggagatttta gttttgcagg
    23221 gttatatctt gctacaggct gtctatatgc ttttctaaaa tatgtggatt taaaaaattg
    23281 gaaattatta ttctttataa taatagaagt atttttatta ggtgcaattt tatgtgttag
    23341 tgcaagaaca gggttagttt ccttgtttgt cacaatgaca ttgtattatc tatttaatat
    23401 aaatagaatt agtgttcctc atcttttagt tctaataggt ggaatcataa ctacacctta
    23461 tatactagta atgttaatgg caggtcgttc tggacaaagt cttttggatt cttcaggcag
    23521 attagaaaac tatatgtcct ctttaaatgc ttttagtgat aaatacttaa taggatatgg
    23581 attaggatta aataatttat acacgctact aggtgtagca gtaccacata atttttttat
    23641 tcaatattta ttacagacag gaattattgg tacgctaatt attttaagtg gcttctttaa
    23701 atttacgtat gaagaattaa agaaatcagg ttgctataaa tggttatttt tgatgatagt
    23761 agtgggagca atgtttatac cggatattgt atcttcgcgt tttctttacg caatagttgt
    23821 tttgtgtatg atttctgcat cagaaagaac tataaaaaaa gaaagttgag gtagaaaaaa
    23881 tgagaagaaa agcagagaaa tgggcagata aagcttgtag acaatacagg aatggtcaaa
    23941 ataaaatgtc aaaaatatcc tgctatattt taaggattat ttatggatgt gaattctacg
    24001 gacaggtaga ttttagctct aatgctcaac tagtccataa tggtcttggt tgtgtattcc
    24061 accctaaaac aaaaatcggt aataattgta aaatatatca aaatgtgact ttgggtggaa
    24121 atggcaaaat aataaatgga aaagaaactg ttggggcacc aacgctagag gaaaatgtgg
    24181 ctgttttcgc tggtgcatgt gttttaggac caattaatat cggacatgat tcaatcatag
    24241 gtgcaaatgc tgtagttaca aaaagtgttc caccatatag cttagtatat ggaaatccag
    24301 caatcataac agagttgaaa tttgattata aatttgatta aaaagtaatg tttaatatgc
    24361 gaaaggttgc tatatttatt tagcaaaatg gggtaataaa tgggaaataa tattcatgga
    24421 aagacagcaa aagctttaaa atggtcatcc ttagctgaaa taattgcaaa gattataact
    24481 ccaatttcga atatgatttt agctagatta ttgactccag atgcttttgg agtagtagcc
    24541 actgtaacta tggttattag tttcgctgat atgtttagtg attcaggatt tcaaaaattt
    24601 ttagtacagc atgaatttga taatgaagaa cacatgtttt catcaacaaa tgtagctttt
    24661 actagtaact taggtatttc tttattatta tggttattta tttgtgtgtt taataataaa
    24721 atagcgcact tagttggaaa ttctggactt ggattagtaa ttatagtagc tggagtttct
    24781 cttccaatta cttctttttc cagtatacaa tcggcaattt ttagacgaaa ttttgattac
    24841 aaaattttgt gttatgctag aataatatct gcattgatac ctttagttat tacagtaccg
    24901 ttggcgataa ttggactaag ttattggtca ctagtaattg gatctataat aggaaattta
    24961 gttacagctg caatattaac taagttatca gagtggaaac caaaactttt tttttcaaaa
    25021 aaagaatttt acgaaatgtt ttcctttagt gcgtggtcac tagtggaatc attaggtaca
    25081 tggttgtcat cttatatagg tacttttatc gtaggaggca ttttaagcgt atactatttg
    25141 ggaatataca aaactacaat gacaacggtt aatggtattt ttgcaattgt aacttcagca
    25201 acaacaactg tattattttc aacattatct cgtttacaaa atagtaaaac ggagtatgat
    25261 aagacatatt tagattttat taaaattgtt tccattttta ttattcctct gggtataggt
    25321 atatatgtat atagggaact agtaacaaat attctactcg ggagtcaatg gaaagaggct
    25381 gtaccatttg ttgggatata tggattaatg agctgtttta ctcttgtctt gggacaatat
    25441 gcctctgagt actatcgtgg tttaggaaaa ccaaaagcaa atgttcttat gactatccta
    25501 catttagcag tattaattcc agttctaata atctattcaa ggaaagggtt tatagaacta
    25561 gcatatggta gatctttcgt taagatagag cagattatag tcttttggat tattttatgg
    25621 tttggtttta agtttaatcc atttaaaatt ttaaagcagg tttggaaatc ttgtgctagt
    25681 gcattgctaa tgggagttct agcaaaaatt atgttggaat tctgtactaa ttatgttatg
    25741 caattacttt cagtgtgtct ttgtgtaatt ctatattttt ttatatatag ttgtatattt
    25801 aaaggaaaaa cagaaataat taatatggtt agaatgttta ctggtaaagg atattaagag
    25861 agatttttat ggcaaggttg tgattaggaa aatgaaaaaa gtaacaattt taattcctgg
    25921 aatgtattat ggtggaatgg aaagagtagc ttttataacg cgtcaaatac ttttgaaaaa
    25981 taattataat gtatcatttg taactctttt tgataacgat gcagattatc agccaaattt
    26041 tgaatattta tcattaaaat gtgatataaa gccatcaaaa tttggtaaag taattaacat
    26101 ttttaaaaga gtttataaaa tgaaaagtgt caaaaaaaaa ttgaaaacgg atataactat
    26161 atcttttgga caaagttgta atttagtaaa tgttttatca aagagagatg ataaagtata
    26221 tgttggaatc cggtcctatg actggctatt tcaatatata ttccattact atgtagataa
    26281 atttgtatat aagaaagcag atgctgttgt gtctgtcagt cagttaatta aacgtgatgc
    26341 tgaaaatatt ttcaatttta ataaggataa aagctatttt ttatataatc catatgattt
    26401 agagttgata aataataaga gtaaagaaaa aattacagaa atagaggttc caacagacaa
    26461 aaaaattata gtgacggttg gtcgattaga agatcaaaaa ggtttatatc atctaattaa
    26521 agctatatct atgttaaaga acaaagataa tgttgttgtg tacatcttag gacatgggac
    26581 aaaaaaagaa ttattagaaa atttaataaa agcatatcaa ctacaaaacg taatattttt
    26641 gctaggtggc caatcaaatc cttataaata catgtcacgt gcagatttat atgtcatgcc
    26701 atctataact gaaggttttc ctaatgcatt agttgaagca atgtctacag aaactgctgt
    26761 cttatgtgca gattgcaaat ctgggccaag agaaatatta tcgaatagaa cgctagacac
    26821 gatttgtgaa aatatagaat atgctgaatc aggcatatta gtatgtccaa tgacagacag
    26881 tagaaattat tcgccccaat atgaagaatg tgataaaaat ttagcgaatg ctatagaaga
    26941 attgattgaa aacgataatt tgaggaataa gtatgcaaaa gcagcaaaaa agagagcaga
    27001 actatatagt tatgatgcct ttgaacataa cttgttgcaa attatgaaga catttaatta
    27061 attcatatga tgaaataaaa ataagggagt ataactagat gctgaaaaaa aatacatctt
    27121 tacagaataa aacaatatta ataactggcg cagccggttt tattggatca aatttggttc
    27181 ttgagttatt acgtactcag tcatcaataa aaatcattgg cattgacaat atgaatgatt
    27241 actatgatgt ctctattaag aactatagat tatcagagat agaaacggag gcaaagaacc
    27301 atccaggatc tacttggaat tttatcaaag gttctattgc tgataaagca acaattgatt
    27361 tcctctttga taattataaa ccaagtatcg tggtgaattt agcagctcag gcaggagtcc
    27421 gttattcaat tactaatcca gatatgtata tcgaatctaa cataatagga ttctataata
    27481 ttcttgaagc ttgtcgtcat tcatatgata atggagaatc tggtgtggaa catttagtgt
    27541 atgcctcatc gtcttccgtt tatggtacta ataagaaaat tccttattct acagaggata
    27601 aagtagacaa tcaagtatcg ttgtatgcag caactaagaa aagtaatgaa tcaatggcac
    27661 atgcatattc taaactttac aatattccgt ctacagggct acgtttcttt acagtatatg
    27721 gtcctgctgg tcgtccagat atggcttatt ttggttttac tgataaactt cgtgctggtg
    27781 ataatatcca aatattcaat tatgggaatt gcaagcgtga ctttacctat atagatgata
    27841 ttgttgaagg agtaaagagg gttatgcagt gcgctccaga gaaaattgat ggggaagatg
    27901 ggctaccaat cccaccatat catatttata atattggtaa caatacgccg gaaaacctac
    27961 tagattttgt agatattcta caacaagagc taattgcagc aggggtgcta ccagaagatt
    28021 acgactttga atctcatacc gaactagttc caatgcaacc aggagatgtg ccagttactt
    28081 atgcagatac tagtgcgtta gaacgagatt ttgaatttaa accaagtaca agtttgcgtg
    28141 acgggcttcg taaatttgcg cagtggtata agtcttttta tgaagtataa ttgtattttt
    28201 ttacttataa aagtttatag aaacacctca gacactacaa atctgaggtg ttatttttta
    28261 atctcgttga aataaatctc tggtatatac tttttctttt acatcatcaa gattactatc
    28321 gtaacgatta gcaataatac tgtgactgat atttttaaaa tcatctaaat tatttattac
    28381 ttttgaacca aaaaatgtag tgttattttc aagtgttggt tcataaatta caatagttgc
    28441 cccctttgct ttaattcgct tcataactcc ttgaatagat gactgacgga agttgtctga
    28501 attactcttc attgtaagac ggtatacacc tatgactacc tctttttctt tttctgcatt
    28561 ccacgaactg ttagcctcat acgtgccagc aagttctaac actcgatctg ctataaagtc
    28621 ttttcttgta cgattactat cgacaattgc acttatcata ttctgaggaa catcgttata
    28681 gttagtgaga agttgttttg tatctttcgg taaacaatag cctccatatc caaaagatgg
    28741 attattataa tgtgtgccaa ttcttggatc taagcaaacc ccgtgaatga tttcttgtgt
    28801 atttagtcct ttcatttctg cataagtatc cagctcattg aagtaagata ctcgaagtgc
    28861 aaggtaagta ttagcgaata atttaactgc ttcagcttct gtgaatccca tgaaaagagt
    28921 ttcgatatct tttttgattg ctccttcttg gagtaaatct gcaaaagtgt gagaggcttc
    28981 tcgtaaagtt ttgtcagttt ggtccgtgcc gacaataatt cgcgaaggat atagattgtc
    29041 atataatgcc ttagattctc tgagaaattc tgggctgaat agaatatttt tagtattgta
    29101 ttttttacga atactttcgg tatagcctac gggtaccgtc gacttgatga ccattattgc
    29161 ctttggattg acttctaaca ccaatttgat tactgactct acagcagaag tatcaaaata
    29221 attttgttta ctatcataat ttgttggagc agcaatgaca acaaaatcgg cttctctata
    29281 ggcggctgca ccatcagtcg ttgcaattaa attaagattt ttttcagcta gatatttttc
    29341 aatgtaatcg tcttgtattg gagatttctt attgttaatt tgttttactt tttcaggaag
    29401 aatgtctact gctgttacct gattgtgctg ggcaagaaga gtagcaatgg aaagacctac
    29461 gtaaccagta ccggctacag caatattctt cgactgatgt tttgttctgt ttgatttatt
    29521 tgattcattt ataaatagat ctgttggttc aaaatttaga acttctgcta atgattctaa
    29581 ttgttgtatt gacgggataa aatcgagaga ttctactcga ctaattaagg cacggttgat
    29641 accagtagct actgcaagtt gttgttgtgt cattttcaga gattttctag aatcaataat
    29701 gatttgagct aaacgttttt gagataactt tttcatgatg cactcctttt tttgttgcta
    29761 ataataacaa acaatttttt cttctataac agaatagctt attattaata aaactgtcaa
    29821 tttttttatt gattaaaaag atattttttg attttaagta ttttcaatta ttttttgtta
    29881 cttaaaataa ctttatagat tgtgaataac tttatacata aaaagtttac gtgagtccat
    29941 acaatataaa gaataaaaaa tgtgatgttt taaactgtaa ccaaccataa taaactgctt
    30001 ctcaattctc cctcatacgc aatacatagg cgtcaagcag gattctcgcg atgatctgga
    30061 gaggcaagcg tgaacgtact tcataatcag ggaaataaga accttctgat ttgaaaccta
    30121 cgagatttat ctcgcaataa tctttgagtg tagaggaatg attggctgta actagaatcg
    30181 aaccaatctc tgcatcatag cagttctttg cggcggtgac gagttctttt gtctcgccgt
    30241 tcaaagaaac aaaaataacg ctgtctttct tagttagttt ccgactgatg cttttgataa
    30301 tctctggatc agtatgcatt tgacaatttt ttcctgttaa ttgaagtttt acgagcatct
    30361 cttgaccaat catttctgaa aatcctcggg caaagacaaa aacccggtca gtaaatttga
    30421 ttctttgtat tgcatcttca atgttcccga tttccaacat attgatcgta cgtaccactt
    30481 cttgttcatt ttttagaatg gctcgacgga tacctttgtc tactttatct aaaaagaaaa
    30541 attgatgttc ttgttttctc cttcttttag atggtgctta aacgaagtaa atccttcata
    30601 gccttttttc ttcattgtcc gcacaatggt ggctgttgag acatttgctt gttcacttag
    30661 tttcacgatg gagtagttag ggatctctag gatgtggtgt tcgataaagt tccagaggta
    30721 ctgttctgtt tcacttaatt tgtgatccat attctttcct tctttcacta aaagttagtt
    30781 aaaaaatagc acgatgtgtt tataaatgta aaggttttct ttgtgttgga acgattaaga
    30841 aaacattttc tctttcgcgt atttcataat agattcaagg aggtcattgg ttatgtctat
    30901 ctacacatgc acgatgaatt tggcaattga tctatttatt gaaacagagg agatgcatcc
    30961 ttttatggta aaccgtacaa aggaggatga cattcaggca aacggaaaag gagtaaatgt
    31021 gtcactagta ttaaagatgc tagatttccc aagtacggca ttaggttttt ctgctggttt
    31081 cacaggaaaa tatatagaag actatttaca gaagaaacaa atccaaacag agtttattga
    31141 agtgcctggt atgacgcgta tcaatgtatt tacacaagtc aatcaaacga gagaagaata
    31201 caaattagtg aatcaaggac ctgaaatacc gcaggcagct gtccataatt ttttaaatca
    31261 aataagagca ttacaagcag aggattatct ttgtgtttct gggagtttac caagaggaat
    31321 gtctccgaag gttttagtgg aaatcagtcg gatttgtcag gaaaaggaga ttcatttgat
    31381 tattgatagc agtgatcaag aggtcatgga ttgtttgccc tatcgaccat ttttgctgaa
    31441 accgaatgag gaagaacttt cttcttggtt cggacgaaaa atggttacag atgaagatta
    31501 ctttgtttat ggacaacgac tggtagagtc aggtgcagaa aatgttttgt tatcccttgg
    31561 tgagaaagga gcgattcttt ttacgaaaga cagggtgatt cgaggaaact cgccaaaagg
    31621 aaaagttgtc aatacagcat gtgcaggtga tgccatgtta ggcacttttt tagtaggata
    31681 catgaatcga cgaccgcttg atgaaacgtt acgtaaaagc atagcagcag gcagctctac
    31741 tgcttttcgt aaaggactga ctgatttttt ggatgtggag gaattaagta aacaaatcaa
    31801 aatcaaggaa gagaggttcg aaagatggga aaatatcaac taattgcagc aaccggttgt
    31861 ccgacaggaa ttgcacacac ctacatggcg caagaggcat tagagcaagc agctagaaaa
    31921 aaaggcatca cgatcaaagt ggagacccat ggacaaattg ggatcgagaa tgaattaacg
    31981 gctgaagaaa tcaaacaggc agatgctgtc attattgccg cagacaaaga tgtccattca
    32041 gaaagatttg cgggaaaacg agtcattgat gtttctgtca gtacaggaat caaggatgca
    32101 caacggctga ttgacgaagc attggcagga aaagggacca tcttagcaga taatgaagct
    32161 gttgatactt tagaatcaga aacgataagt ggtgctaatt ttggtcgcag catctataaa
    32221 aatttaatga atggcgtctc tcatatgttg cctttcgtgg tagctggcgg agtattgatt
    32281 gctatttcat ttgccgtttg gggaatctat tcttttgatc cagaccatgc acaatacaat
    32341 gctacagcgg cgatgttaaa aacggttggc gatgcagcaa tggggatgat ggttccagtt
    32401 ttagcagctt atattgcaga agggatcgcg aaacgtccag ggttagtcgt tggatttgtt
    32461 ggtggaatcg tggccatgag tggcggtact ggatttttag taggtatttt agctgggttc
    32521 ttagcaggtt attttatctt atttttgcaa aagctactga caattttacc taaacaacta
    32581 gatggcttga aggcaatctt cttatatcca gttattggtg tagtgtttat cggcatcgtg
    32641 atgtctttgc tggcgggtcc aatgaaggct ataaatgaag gaatgatgag ctttttagct
    32701 ggatttgaac attcgaatcc acttgttctt ggcgtgattg ttggttgcat gtgtgcgttt
    32761 gatatgggag gaccgattaa taaggcggca tatgtaacag gtacagctct tttggcacaa
    32821 ggaaacacga cttttatggc tggcgtgtca gtagcttgca ttgcaccacc gttgattact
    32881 ggttttgcaa ctttattgtt tggtaaatat tttgatacta atgatcgtaa tgcaggtttg
    32941 gttaatttta ttttaggatc aactcatatt acggagggag ctattccgtt tgctgcgaaa
    33001 gacccgatcc gagtattacc aacgatgatg ttaggctcat caattgctgc tgtcttgacc
    33061 tatctgtttg gtgtccaagt accagcccca catggcggtt ttttagtgtt acctgtagtg
    33121 acgggaaaaa tccaatgggt tacagcgatt cttattggtt cgatcgttgg tggagtgata
    33181 ctaggtttga tccaaaagaa aagagtaact gataaagaca agaagatgga taataaagca
    33241 gcaaatcgta cagttatcga agaaggaaga gcaggaggct tatcatgaca atcattcatg
    33301 aagagaacat ttttttacaa cagccattag atagtcaaga agaagcattt aagtttttag
    33361 caaagcaaac attctgttta ggaatcgctc atgacgaaaa agaagtatat cgaaaacttc
    33421 tagaaagaga aaaagaaggt actacaggaa taatgtcagg ttttgctatc cctcatgcga
    33481 agtctcaaac aatcaacgaa cctagtatga ttatcgtcac actgaacaaa ggaattgaat
    33541 ggaacagttt agatagacag tcagttgatt ttatttttgc gttatttatt ccagattcag
    33601 aagcaggaac gactcatttg aaattattgt ctctcgttgc acgatcattg atgcgaaaag
    33661 aggttacaga acagttgaga aaagcaaaat caaaaacaga aatagccaca atattaacta
    33721 aagaaatagg tgaagatcta tgaagaagat gagtatagaa aagcaggaac gtttgaagcg
    33781 attgacggat gaagctggaa ttattagcgc gttagcaatc gaccagcgtg gtgctttgaa
    33841 gaaaatgatc aaccaatata aagaagctac atcagaagac atcatccaat ttaaacagat
    33901 ggtttctcaa tacctgactc cctatgcctc agctatttta cttgatccag aatgcggcct
    33961 gcctgcagca gcgcttagag agaaagaatg tggcttgctg ctagcttatg aaaaaaccgg
    34021 ctatgatacg aaaatgccgg gaagattacc tgatttattg aatatttggt cggtgaaaag
    34081 gttaaaagaa gctggtgcgg atgcttgtaa atttttattg tactatgacg tggatgaacc
    34141 agcagagatt aatgtgcaaa aacaaatagt tatagaacgg attggttcag aatgcgatgc
    34201 agaaggcata cctttctttt tagagatcct ttcttatgat gcaactattg gcgatccgtc
    34261 ctctcttgcc tatgcaaaag taaaacccca taaagttatt gaagccatgc gagaattttc
    34321 aaaagaacgt tatcaagtgg atgtattgaa agtagaagtg ccagtgaata tgagatttgt
    34381 ggaaggatat gggcaagaga cttgttatag tcaagcagag gcatccgcgt atttctatga
    34441 acaatcgaaa gtgactgaac aaccgtttat ttttttaagt gcgggagtca atgctacgtt
    34501 atttcaagat acattacgtt ttgcaaaaca atcaggttcg acattcaatg gtgtcttatg
    34561 tgggcgagca acatgggcga agggagtggc accttttgtt accgaaggcg aagaaaaagc
    34621 gagtagttgg atgttagaag aaggaaaaca aaatattaag gaattaagta cactattaaa
    34681 agaaacagca acacctgtct acgttgatag ataagaaaaa gttgtttctt cataaaaaga
    34741 tgctgctgca attagatagc gatcaatcga tgattcaaat tatacattca cttaaataaa
    34801 aatatcgact agatttcttt ttcaaaaatc ttggtcgata tttttaattg ctaaaactgt
    34861 taatgaattt ttattacact cttatttact atctacttcc gcttctgctg ctttcctgcg
    34921 ttgcggcctt tgttatttgt actgtttttt ggttgtttga tttgttttgt tgcttgtttg
    34981 ttgtcagctg gttttgctga ttctgtcacg tcttttcttg gagtgacgac ttgttttggc
    35041 gggttttgtt tcaactcttc tgccacttgt gctttcaagc gtggtttcat cataacgttt
    35101 gtgatgaatg tttgcagaca gctgaacaca ccaccgacta cccagtaaag tgtaacgccg
    35161 gctggcgcac tgatcgacat gaagacgatc atagcaggag agacgatcag catcgtacgc
    35221 atcgtctttt tctgttcttc aggaataccg attgttgaaa tatatccttg aagcagataa
    35281 gcgacccctg caatggccac aagcaccata cttggtgagc ctagatcgat gccgtaaaaa
    35341 gatgattcac ggataccttc tgtgtagcga gccgtaaaat acaaggcaga gaagatcggc
    35401 atttggatca atagaggcag acagccgatt ccgccggtca tactcaatcc gttttcgtga
    35461 tatacttgct gcatttctgc ttgtgctgcc atttgttctt cacgtgttgt tgcagctttt
    35521 agcttttgtt gtgctgcttc aacttgtggt tttaacgctt gcattttttc agattggatc
    35581 attgtttttt tcgattgact gatacctagt ggcaagatga tgatacgaac gatcacagtc
    35641 attgcaataa ctgcccagcc gtaagaccaa ttgaaattct caactagata tgtaatcgct
    35701 tggcccatag gatggacgag tactcgataa acgattcctg aagtgtcagg gtttccgttt
    35761 gaatccatcc gcacacagcc agataaaaag agcaggatac tgacaagtcc ggatcccaat
    35821 aaccagtttt tccatttatt catagaatac ttcctcttct ataattttta cacaaactta
    35881 actatactaa aaaagcgcat ggaattcaat caacttcata gaaatttcat ataaatattc
    35941 tttctttttc gtagcgaaaa attttgaaaa aagacaaaaa atcaagaaag aaatacttgg
    36001 aaagaaaaga gtgattccaa gtgtttcttt cttatataaa aaactaaaaa cttttttgtt
    36061 ctgtctggaa aatatgggtt aagcaaattt attgcaagtt atagatcagc ttttttacta
    36121 cctatcttta atacttgagg caaagcgcct caaaaacaac ctgatcgacg gtgttcaaaa
    36181 gtcagtttct cggtattaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    36241 ttttccttaa tactcaaaac taagcgacct tttcacaacc ttatcccacc acatcaaatt
    36301 tctttcgttc ctcaatcatc ggatcttctt ggacatccac atactgcact cgtccacttg
    36361 ggctcggaga cttcttgact tcttcgataa atttttggat gatgtcgtca tttccgattg
    36421 cttcgattgt gactgagccg tcgtcttcgt tttttaccgt tccggtcacg cctaactgat
    36481 cagcaaccat cttggtcata tagcgaaatc ccacgccctg gacacgtcct tggacattca
    36541 ttttaacttt gcgcatgttc gtcacctttc tttcctgttt tataaatcaa tgataccgat
    36601 ttttttagga tgaaacaagc tcaagcacct attccatgat ataatcaggt gaggtgaagg
    36661 aatgtgaaag agatccaatc agcaaaaaat ccaaaaatca aagaattaaa aaagctacat
    36721 aaaaagaaat acagagaaga aaaggcagaa tatcttttgg aagggttcca tctgatcgaa
    36781 gaagctgcgt cgtctgatgc agaaataaaa gaactttttg tgacacagag aggtatgaaa
    36841 gaatggggag actggctggc tgtaaacaaa gagatttcta cttttctagt ttcagatgag
    36901 gtcatgaccg ctatgtcaga tctacctaca ccgcaaggga tcttagcggt gatgacgtta
    36961 acatctgaag atacgccaga tttttcagga ggctggcttt tactagataa tgtacaggat
    37021 cctggaaatg tgggaacgat gatccgtacg gcagatgcgt ttggtatggc tggtgtttta
    37081 ttaggtaaag gaacggcaga tatttatagt acaaaggtgc tgcgaagcat gcagggaagc
    37141 aattatcatt tgcctgtgtt acaaaaagat ttggcagaag tgatccctgt aatgaaaggc
    37201 aaagggttta cggtgtacgg tacagaatta aatgaagaag cacatgcatt agacaaagta
    37261 gaaaaaacag aggatttcgc tataatcatg ggaaacgaag gtcagggagt aagtcaggaa
    37321 atcttgtctt tgactgatca gaacgtatat atcccaatga aaggaaatgc ggaatcattg
    37381 aatgtcggag tcgcagcagg gatcgttctt tatcattttt ctttatgatt aaggaactat
    37441 taagctttag ctattttatc tagagcgact agaataatcc gaacactggt ggtatactta
    37501 gatcaatttc agaagaaaat gaaattcaaa aaggtggcag atcgataatt atgacagaaa
    37561 aatggcgtga agacgaagaa tatatgtctt atgtacaaga tcttttagaa acagaagcag
    37621 tgaaaaaatt agcgaattac acacagcatg ttcattccac tcgtttggag cattccatca
    37681 gtgtttctta ctatagttat ctgctcgcaa aaaaatgggg tggcaatgct aaagcaacag
    37741 cgcgtgctgg acttttgcat gatctgttct actatgattg gcgtacgacg aaatttgacg
    37801 aggggacaca tgcgtatatc catccaagaa ttgctgtaaa gaatgcagaa aaaatcaccg
    37861 atttgtcgga tcttgagcga gatatcattt tgaagcacat gtggggtgca acgatcgcac
    37921 cgcctaagta taaagaagga tatattgtga cattcgtgga caaatactgc gcagtcaaag
    37981 aagcggctca gcccatgtct gcaagtatgc gtaagagatg gcaacgttac tttgggaaag
    38041 aatcctcgat ttaacaggta aaaccaataa tagaccatat tgaggttgtg acagaagtgg
    38101 tcagcttcaa gaaataagaa ggaatttacg aaaattactt ctcaaatttt tgtgaagttt
    38161 cggcttatta ccgcagatgc aagcttttga acaccattca ttcgaaacaa gggactttga
    38221 cacgactttt gtcacagtcc ttttttctgt ttgcagttga ctatctgttc aagatacggt
    38281 ttacttttat ttatcgaatg aatcatgtac atagaagaat aagcattaaa gggggtgatg
    38341 ataagatgca aaacgtatct tatttaaaag agtccgggat cttcaggtaa cacatacttt
    38401 gcagaagatc acttttttct acaaagtatg cactttggtt agaagaaagg actttaaaat
    38461 catggaacaa acaataaaac cataccaata tttttttata aaaagagaag tagaacagtt
    38521 attaaatgcg tactcatctg tcaatgatcc aaaaacggta caaacagttc aggcactagc
    38581 aactgaaaag attcgagaca tattagaaca cgaactgcca gaaatcgata catttttaac
    38641 gacagtttta gacgtaaaac tgacaaaagc gcaagcagaa cgagcattgg aggagctgaa
    38701 gcaaatcgtt cagccgttta tgcagcctag caaaccacaa atcgagaaat tattccgaaa
    38761 agtgaaaaaa ctcaaacaac cagcttggaa tgaaatcgac ttgcaagaac atacgtacat
    38821 cggctggaat gatccaggta cgcagaaaaa attcatgatc tattacgaag atgaaaaatt
    38881 aaaagggatt agtggtactt tatcaccaac gataaataag ggaatctgtg caatctgtca
    38941 gaaaacatcg aatgtttcgc tttttctttc tacaacgaaa gcaggttctg atggcacgta
    39001 tacgaaaaag gggaattata tttgtcatga tagtgaccaa tgtaatcaac agttaacgaa
    39061 attggctcct ttctatgaat ttgctgaacg tgtaaagaaa gaacgataat tttattgttt
    39121 tttacagaaa aatatgctat cttttaatgg aagtgaatag gtgatggtgt tcgcctttaa
    39181 atgctaacga aagatagcta atgacgccta ctaaagtctg gggacagccc ctttctttag
    39241 taggcgtttt tttattgagg aggaattttt tatggaaaaa caattaccga gtccagtacg
    39301 cattttagtg tacatgctga agtggctggt gatcacagcg attctcggta tattcatggg
    39361 gagcttgtct gcttttttct taaatagcct gacagttgtg acagatatcc gactatcaca
    39421 tccttggctc ctttatctac taccagttag tggtgcgctt tttgcttatc tctatgcgta
    39481 tcacggcggt ctttctagtc gtggaaacaa cttagtgatc gatcaaggaa atggtggaga
    39541 agaaaaaatc ccgttgcgac ttattccact tacactgttt ggaaccataa ctacccattt
    39601 atttggggga tctgttggcc gtgaaggaac agcagtccaa atggggggag cgttagcaga
    39661 taatacagca cgtttgttcc gattggataa atcagaacgg gaaatcttga ttatcagtgg
    39721 aatcagtgcg ggttttagtt ctgtatttgg cacaccttta gctggaacat tatttggatt
    39781 agaagtgttg gcaatcggga aagtccgaac agaagcgatc ttccctagct tctttgctgc
    39841 actgtttgca aatttcatga cagaaagcta tggtgtttct catctacatt accaaatggg
    39901 tgtgattcct gaatggagta tgttgctgtt cttcaaagtt tttgttgctg ggattgcttt
    39961 tggtttgatt ggttgggtat tcagccgttc gatcgtcttt ttaaaagcgc gatatgcaca
    40021 atggattcct catcctgttt ggcgaaatac gataggtgga gctgtagttg tggcagcagc
    40081 attgattttg caaacacaac gttatctcgg attgagtctt ccgttacttc aagaatcatt
    40141 taatggacag gcacatgtct atgattttat tgggaagtta ttcttcacag ttttgtcttt
    40201 aggcgcggga tatcaagggg gagaagtgac accattgttt gaaattggtg caacgcttgg
    40261 tgcagcacta gcgcctttgc tccatgtttc tgttcctttt cttgcaggat tgggctttat
    40321 cggagtattt tctggtgcga caaatacacc gattgcctgc tttgtgatgg ggatcgaatt
    40381 gttcggttca caagcggcag tatacttctt tatgatctgc ttgatcagct ttatgtgttc
    40441 aggcaacagc ggtatttatt ctgctcagca agtagcggtc aaaaaaggga tactcttttt
    40501 gccaatagtt aagagaatga aagaaaaaaa caaggtgtga catatgtttt gtcacacctt
    40561 gtttttttag aaaagtaaag taatcagcga taagaatgcg gagcctgcga aaagcaatcc
    40621 gcaaatgatc atatgtcttt ttttgttcat catcaagatg gcgatgtatt ctcttaaaaa
    40681 cgcattaggc aagtagtaga aagctgtttt tgcaccaata ccatttgctt tcaatttggc
    40741 ttgccacgca taaagaccgg ctctaaaaat gtggtaattg ttggaactaa agatcgcgcg
    40801 gtatggccct ttcatttgct gatccatgat ttcctttgaa tagagcatgt tctccaaagt
    40861 ggttgtcgaa ttggtttcga caagaatgtc tctttcaggg attccttgtt ccatggcata
    40921 ttgtttcatc gcaatggcct ctggtacttt ttcgtctgct ccttgcccgc cagacatcaa
    40981 taagatgggc ggatttaatg tagcacggct ttgagcccga taaaaagcaa ttgccttatt
    41041 gattcgcttg gctagaagag gagaaactgt ttctccattg atcaacccag cgcctaacac
    41101 aacgatgaag tcttgattgt atcgaggccg gttgaactga tacaagaatg agacagtcaa
    41161 aaagttgtag aatacgataa atagatagat caagatcagg ggaacgcaga aaaacaaagc
    41221 atttatccat tgcggcaaat attttaacaa gaaaaagtca aaaacaagaa acagtgtaag
    41281 aaaaatggct agtatcaacg ttaaaagatt agctaatgaa tgcgattctc tacgtaaaac
    41341 tgtgaccccg ttccaaaaaa gaaaaacaat caatccataa ataccaaata aaaggacaag
    41401 tagcaatgga atcgtgatca aggcgatcag tccgccaagg aaaatatttt gcgtttcata
    41461 cagcaagaca aacaagtaga tgccgaaact gatgaggaaa acattgaata aaagaccatt
    41521 cagcagcctt cgtttttctg tgaaataaga aaaagcaaag atactaaaga acaacaatgg
    41581 aatcaaaaaa taaaaggtcg aataaaaaat ttccaacacg acaaaataag ttaagattac
    41641 cgtaaatgtc cacgtactgc cgtaaaagaa caaggaagga tccttcttct gttttcgcca
    41701 ataaacgata gagaataccc caataagata gagaactaaa aagatcagat ctctggttag
    41761 attcataaat tcaactcctt tttcttatac agtataacga aaatagatga aaaaagctgt
    41821 tatttaaact agaaaaagca aaaaaacatc tttccgatgg aaagatgttt ttcttattgt
    41881 tcaattaaaa caagttgccg aaaaattctg cgacgcgtcg ccagccaatc acgaatatat
    41941 tcgctttttc cactgtttta tccgtaatga tctctgttga aggtgcattg ttgtcttcta
    42001 aatagcctaa tttatcgtct gccaaggtga tcgtagcggt accgattttt gtacctttat
    42061 ccataggtgc ttggatctca ttgtcagtga cttgcttctt gtcaagtgtt ggcgtgatcg
    42121 ttaatttgcc agtatccata tcgtttcgta cccatacttt tacagggctt ttaagcacaa
    42181 caggaactgt tgattctttt ccgtctttta catcaatggt ttttagacta ggaatacttg
    42241 cgttagcttt tcctagctct ttttctgacc aattgtcata gcagtagtcc atcaaacggt
    42301 ctgtttcatt gaagcgggca ctcgggtttt ccgcatggcc gtccgcattc aatacgacag
    42361 tgatgatccg ttgatcgttt ttagtgatcg taccaacgaa acaagcacct gcaagctcag
    42421 tcgtaccagt ttttaatccg tccacgcctt ccttgtaatt aacgaatcct gggagcatcc
    42481 agttccagtt gaccatttcc actggtgatt gcgtattttc gccaaacatt tgggtcgtag
    42541 tacttgatac atccagtatt tctgggaaat ccaagatcaa atggcgggca acgatggcca
    42601 catcttgagc agacatttga ttttcgtcgt tttctccagt gccttctggt cggttttcgc
    42661 caagataaga gttgttcaat cctgatgcat taacgattgt tgcatctttg attccccaat
    42721 ctttcaattg ttctttcatt cgttcagtga atttagcttc gctgccagag attttttctg
    42781 ccaaggccac cattgaagca ttggctgact ggatgattgc agagtcgaat aattctttga
    42841 cagtgtaggt gttttcttta tgcaaaggaa cattcgataa atctggggtg atactcaaat
    42901 tttctgcata atctgaaatc gaaactttat catcccatga cagttttcct tcttttactt
    42961 gatcaaggac gatgtaaaga ccgatgattt ttgtgattga agcgatcccc atggtttttt
    43021 ctccatcttg atcataaaga atcttaccgc tttgcgcatc tacagcaaaa gctgcttttg
    43081 catttacttt gaacgtatct tcagcttgca cagcaacagc tggtagaaaa gaagcaaggc
    43141 aaagaatgag actagccaat aataggacta gacgtgtttt ttttatggac atatccgatc
    43201 tccttgtaat taatttgttt cgctacaaat taaaatttta tcagagaggg gagagagttt
    43261 caatgtttct gaaaaaactt taggataaga acgcgcgtta tggaagttta gcggtcgttt
    43321 ttacgtactt ttacaggaat aggctgtgtt tccgcatcta ataagcggga agcgctgaaa
    43381 gcaaataaac tgataatact taataaactg aaaatcataa tcaaatgcat aagatccacc
    43441 tcttttattc tttttcttag tttatcagca tttcatgaag aatagttgaa gactggggct
    43501 aaataattga ggaaattatg aagataactt aaaaaaatag ctaaatttaa aaccgtagaa
    43561 accgttttat ttttttagat acgttttatt gtatggaaaa atagtgttgt ggtagacttt
    43621 agatagaaac aaaggaatga tttttttagg aggggtacgt ttatggcaaa aaaggataaa
    43681 gaattattga cacaattgac ttcagaaggc gctaaaggac cattcgtcac gatcatgctg
    43741 aatacgcatg tcgcccatca agatgtggaa aaagatcaga tcaaattcaa aaattttgcg
    43801 aaagaagcaa agaaacgttt tgagaaaaaa tattccgaac atgactggtc cccgttccaa
    43861 gaaaaaattg aaaatttgtt aggagatcct tcgttttggc gtagtgcaac tacgagtgta
    43921 gcggtcatta taactgctga ccagacctat atccatcgtt tgagtatccc ggtagacgat
    43981 caatactatg taagtgatct tccttattta ctggctatta tcaaaaatgg gcagttcaac
    44041 tattcttatt atttattggg attgaaccgc gactccatga aattgtatga ggtcagcaac
    44101 aaacaagtaa aagaagtatc tctaccagaa ggggcaccgg ttgatgtcgt tactgcattg
    44161 ggatctgaac tgacaggtgg cggtttgaac ttctcttcta atggaggagg cggagcccga
    44221 gatggagtaa ctttccatgg cgtaaatcca aaagatgaag aagtagagat cgattgggtc
    44281 aactattacc aagctgttga tacgtacttg aaggattctt tcgaaaatcc tgaacaatgg
    44341 ccgatttatt tatttgcatt accagaaaat cagacgatgt tcaaaaaaat tgctaaaaat
    44401 ccctattatt gtgcagacgt tgcgatttct gcttctcctg cgcagctatc ctttagagat
    44461 atcgaagaag gggcgaagaa gctgtcaagt gaactcgcag ctcaagaatc aaaagaatac
    44521 aataaactct tagataagaa attcatcgat cagttagttg atatcgttcc agctgcgaaa
    44581 gaaggaaaag tgtcacattt atttatcgct acgtcaaact tagctgatgg tttcggtgaa
    44641 gatcctgaaa cagaatatga tcggcgtcaa gtgttgaatg cattagcaga tgatgtgatc
    44701 attaatggcg ggaaagtgtt tattttggat caaaaagatg cgccagatga aaagagtctg
    44761 acagcgattc ttcgttatta aaagaggttg tgaaatcagt gttttgacct gagtattaga
    44821 gaaacaaacg gaaaaatagt ttcacatatt tttccgtttt ttgagctaat accgcagatg
    44881 caagcttttg aacaccgttt aaggaagagg ttgtgacaga agtggtcagc ttcaagaaat
    44941 aagaagagat tgacaaaaat tatttctcaa atttttgtga aatttcgact tatttccgaa
    45001 gaagctactt ctggaacacc gtttaaaaaa gaggttgtga cgtggtcacg acctcttttt
    45061 gacgattatg atacaaaaaa catcatttat gaacaaaatg tgaataacgg gcgttcttga
    45121 gatatgattt taattgggaa aaacttatcg ataaggaggt ctttatgaaa aaaagactgt
    45181 tatggtgttt gctgtttcta tccctaagtt taacaagtca gccagttttt gctgaagaaa
    45241 cacagcggtc tcctattaca ggcacacgtc tgtacaaaaa tgtttatgta cagacaacag
    45301 atcttgattc tgcgattcct gaaacagaag aacggtcttt gcagccaaga tttggttact
    45361 tttggtactg tccgacctgt gattatgaaa gttgctggca tttcttacat tctacagctg
    45421 cagcttttgc ttttactcat actcagaaat atgatggaca tcatcctgtt atttatgagg
    45481 tatagatatg agcaaaaaag aaaaactaat ctcgttgatc gtattggttt gccttgttct
    45541 tctggtaact gtcataggct atcagaaata ccatgagaac atcagaccta ctaaagaatc
    45601 ttccccagcc tcgaccgttg aaacagaacc gataagtagt cagaaagaat aattttttta
    45661 gtttaaagaa gaacatcaga gcaaaaaaac accaactgaa caatatgtcg tttattttaa
    45721 aaaaagttcg gtttttcact ttatgtaatc gctgagaaaa ctttttttaa taaaaaaagt
    45781 cgaaaaactc tagctttttt gtaaacggta tgatatacta taggagtagt ttttgttcgg
    45841 taatctgtgg tagcttgttt caaataaaca tcttccagat atacaccatt caagtaattg
    45901 caaaatatgt gttgatacac aaggaggatt tttattatgg aaacaggtac agtaaaatgg
    45961 tttaactcag aaaaaggatt tggattcatc actgcagaaa acggaaacga tgtattcgtt
    46021 catttctcag ctatccaagg cgacggattc aaaactttag aagaaggcca agcagtaact
    46081 ttcgatgttg aagaaggcca acgcggacct caagcaacaa acgttactaa agcttaatta
    46141 acctatacta aaaaataaac gcccagacat ttgcgtgtct gggcgttttt gtctattttc
    46201 tttaaaaagc gatgagagta tttttctgca ctttgtagta taatttcaaa agaacattag
    46261 aggcaggaga agaggaagat attttgaaac caagaactta tgcagtcgtt gatatcgaaa
    46321 cgactgggac gaatccaaaa gaagatcgga tcatccaatt tggctgcgtc atgattgaat
    46381 caggtcggat cacttcacgt ttttcgatcg atattcatcc agggcggaag atttcaaaac
    46441 aaatccagca tttaacaggg atcacgaatc aacgcgtcca aaaagcgcct tatttcgaag
    46501 atgtcgccca aacgatttac aatcttttgg cagacaccat atttgtcgcg cataatattt
    46561 attttgatta taattttttg aaccatgaac tcatgcgatg cggacttcct agtttgaaga
    46621 taccaggcat cgatacagta gaacttgcac aaattttctt gccgacggaa ccaagctttc
    46681 gcttagctga tttgtcagaa agccttggat ttattcatga gaatcctcat caggcagata
    46741 gtgatgccga agtgacgggt caacttcttt tgctgatcga agaacggatg agaaaattgc
    46801 caatcatcac gttagagcag attgcgcgat taagtagaca cacaggtatg gatacgagtc
    46861 gtttcattta tcatgtgatt gaagaaatga aagaaaaacc agagccactt gatccctcat
    46921 tagaaatcgt cgatgggcta gctttgcaga aaaaagaaat ggaactattc atatacgtcc
    46981 attatggcga gcggacatat ccaagaaaaa aacaagcaaa agaaaaaatg tttggtaaaa
    47041 cactgatgta tcgaaaagaa cagaacagac tgatgaatgc ggtctacgac catttcacaa
    47101 aagatgaatc aaaagatttg atgatcgaag cagctacagg aatgggtaaa acaatcggtt
    47161 atctgctgcc attgagttat ttggctacac ctgaaaaacc cgctgtcatc agtaccgttt
    47221 cgctagtttt gcagcagcag atcatagaaa aagacattcc attgctgaat caattgctag
    47281 atcagcccat acaacctgta attatcaaaa gctatcgcca ctatattgat ttacagcgtt
    47341 ttaaaggcac acttgtggaa ccgccagaac aaaaacaata tgctttgtat caaatggcgg
    47401 tcttagtttg gctgacgcaa acgaagaccg gtgatttgga tgaattacat ttaacaaatt
    47461 tgaaccattc attctttgca gatatcgcgc acagaggcac tggattttta gcaagaaatc
    47521 agtcatttta cgaacaagac tttgttcgtt tcttgcaaaa gaaaatcagg caaagtaatt
    47581 ttttgattgt caatcatgcg tttttaatac aagaaacaca gcgaaaagaa ccattgcttc
    47641 cagctagtcc atttttgttg atcgatgaag tacatcattt gccggaaatc gctgaaaaag
    47701 taaatgacaa acgattaagt attaactatt tccataaaca aatccagcaa ttagaagaac
    47761 caaatcagct gtttgaccga atacaaagct atttaccgaa agaacatgaa gccttacgct
    47821 ggttatcgat ttatcgagaa gaactttctg cgctatgcga agcacagatt tgtatggcag
    47881 aaggattaat gagtttagtg agcggcaagc aacctaacgg acagtatcca gaagaagtca
    47941 tgacgacaaa agagatgttg gatcagctgc cactggaatc tgggacagcc attgatcacc
    48001 tgcagctttt ttatcaggag atccagcagc tacagtcaca aatgcgtctg acttttgaga
    48061 ttatccaaga aagctggaca aaccagatgc ggatcgattt agcaagttta tatagcttat
    48121 ttgatcaatt agacaagcag tacttgctga tggatcaatg gctggaagac tggcgagatc
    48181 atttgatcca ttggttcgtt cctgggaaca cagcgcagca gatgattttt catctccatg
    48241 attttcaagc ggctatgttg ccgtcgactg tttggtatcc tcgatatgat cgtattcttt
    48301 atattggagg aacgctgaag atcggcccta atcgacacta tctctctaag cggcttggca
    48361 ttgaagcagc acctcttaaa gtgattgcct ctccttatga ttatgagaaa caggcaagga
    48421 tctatgtgcc aaaagaggca cctgccgtcc atcaagtcag caatcaagca tatatcgatt
    48481 atttagagac agcacttaca ggtctgatcg aacaagaaga tcggccaata cttgtgttat
    48541 tcacttctca tgatgttttg cagaaggtct atcagaaaat gcatctgtct atgctagaaa
    48601 aaggacgaga aatactagct caagggtttg gcggaagccg ggagaagctt ttgaaacgct
    48661 tcattttgtc agaaaaaagc gtattgtttg gcgctgacag tttttgggaa ggtgtagatt
    48721 tgcctggaga tgctttgcag atcgttgtag tcacacgttt acccttcgat aatccgcttc
    48781 gcccgatggt caaagctagg aacgcttatt tagaatcaga ggggatcaat cctttttacc
    48841 aggaatccgt acctaaagca gctttgaagt tacgccaagc attaggacgc ttgataagga
    48901 cggagacaga tcgtggcgta ttgcttattt tggatcgaag actagttact gcaaaatatg
    48961 gtgcacggat catccatgca ttaccgaaaa aagtaccgat caaagagcta gctatggagg
    49021 aaatctatca ggatatccat gaattcttaa agaaaaatga agaaggaagt tagaggagct
    49081 gtcttttttc ataattaaaa tctgctataa tgaaaaggaa tgaagataaa ggagcgacga
    49141 cccttgaaga aaaaacagac aaaggaaaaa acgattaatc gcgtgttact tggcttgatc
    49201 gtatttttat tagcggtcat cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg
    49261 gcggtgcagg cgaagaaaga agcagtagaa atcgcaaaga aatatgcaaa tcttgaggaa
    49321 gccgatcatt tttattggtt tactcgaaag aagacatact tttcactgac aggtacaaat
    49381 gataaaggaa aagaaatcgc cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg
    49441 gatcaaacaa agggactgac tgaaaatgaa gcaaaacaga caattgctgc ggcccatcct
    49501 gaaatccaaa tcgaaaaagc tgctttaggg atgtatgatg atcagcctgt gtgggaagta
    49561 gtaggaaaag aaacaaatgg gaaactgaat tattacctga ttggctttga atctggagat
    49621 gaagtcaaga caatcaaggg aatttaaaag gtaaaggaat gaatgggatg gagttatcaa
    49681 aaagaacaaa acgattagaa ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat
    49741 taaaagccaa aggcagagat gtgctgagtt tgactgtggg agaaccggat tttgcgacac
    49801 cagaaaacat ccaagaagca gcaatcgaag caatccgcaa tgggaaagct agctattata
    49861 caccaacagc aggaatccca gagcttcgac aggcaatcgt tgattatctg aagaaaaact
    49921 atggacttgt ctatgaacct tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt
    49981 ataccctttt ccaaacgatc cttgacccgc aagatgaagt catcattcct gtgccttact
    50041 gggtcagtta cggtgagcaa gtaaaactgg cagaaggact tcctgtcttt ttgaaaggag
    50101 aagaatccaa tagctggaaa gttactgttg agcagctaga acaagcacgt tcagaaaaaa
    50161 caaaagccgt aatcatcaat tctccatcga atccgactgg aatgatttat agcaagaatg
    50221 aactgcaggc aatcggtgaa tgggcagtaa aaaatgatat tttgatagtt gctgatgata
    50281 tttatggccg tttagtttat aatggcaatg agtttactcc aattgcaacg atttcagaag
    50341 caatcaaaaa tcagaccatc atcatcaacg gcgtgtctaa aacatatgcg atgacaggct
    50401 ggcgcattgg ttatgcggtg ggaaatcctg tgatcatcaa tggaatgatc gctgttgctt
    50461 cccaatcaac tagcaatccg acagcagtca gtcaatatgc agctgttgaa gcattgaaag
    50521 gcgagcaaga tacagtggaa gaaatgcgga acgcctttga agaacgcctg aatacgttat
    50581 atccgcttgt ggcagaactg ccgggtgttt ctcttgaaaa gccacaagga gctttttatc
    50641 tttttcctaa tgtaaaagaa acgctacgca tgtgtaaata tgaaaatgtc acacaatggg
    50701 tagaagatct tttagaagaa acaggggtag cgcttgtgac tggcgaaggc tttggggcac
    50761 cagaaaatgt ccgcatgagt tatgccactg atcgcgctac gttagaagaa gcggtacgca
    50821 gaatcaagca atttattgag agtaaaagtc aaaattaatc ctgaagaaat tcaaggaata
    50881 taaaaggaga gtcatacgtg gaacaaattc aaatcatcga ttcgaaaaac catgtgggag
    50941 aaacagtaac aatcggcgct tgggtcgcaa ataagcgatc aagcggaaaa atcgcatttt
    51001 tacaattaag agacggaaca gcctttttcc aaggtgtagt tgtaaaaagc gaagtgccgg
    51061 aagaagtttt ccaaacagct aaaaatctgc accaagaaac atccgtgtta gtaactggag
    51121 aaatccgtga agacagccgt tcaaaatttg gttatgaaat cggaattaca aatgtggaag
    51181 tggtaagcga gagccacgat tatccgatca caccaaaaga acacggaaca gacttcttga
    51241 tggatcatcg tcatttatgg ctacgttctt caagacaaca tgcgattatg cagatcagaa
    51301 atgaaatcat tcgtgcaacg tatgaattct ttaatcaaca tcattttgtc aaaatcgatc
    51361 cgccgatctt gactggttcc gcaccagaag gcactacgga tctattcgaa acaaattact
    51421 ttgatcaaaa agcttattta tctcaaagtg gccaattata catggaagct gctgctatgg
    51481 ctttcggaaa agtcttttca ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac
    51541 gtcatttgat tgaattctgg atgatcgaac cagaaatggc tttcactcat caagaagaaa
    51601 gtttagaagt acaggaacaa tatgtagcat tcttagtaca aaatgtttta gatcattgcg
    51661 aatacgcatt agatgtacta ggacgagaca aagaagtctt aaaaacttat acaaaattgc
    51721 cattccctcg tattacttat gatgatgcga tcgaattact gaagaaaaat ggatttgatg
    51781 acatcgaatg gggcgaagac tttggttcac ctcatgaaac gttcattgcg aattcattcg
    51841 aacagcctgt ctttatccta aactatccaa aagctatcaa gccattctac atgaaaccac
    51901 atccaacaag agaagacgtg gtaatttgtg cagatatgat cgcaccagaa ggttacggtg
    51961 aaattattgg cggaagcgaa cgggcaacgg actatgattt cttgctagaa caaatccgta
    52021 atcacggttt ggatgaaaaa gaatattcat ggtatcttga tctacgtaaa tacggttctg
    52081 ttccgcactc tggttttggt ctaggccttg aacgaacagt cacttggatt gctggtatcg
    52141 accatgtgcg tgaagctatt ccattcccac gtttgttgaa ccgaatttat ccataagagg
    52201 tgatgaaaaa gctgttctgc atcaagtaat tagaacagcc aagcaaaaac agctgttcat
    52261 gtttttgaat tggcgggact tattaccgca gatgtaagct tttgaatacc gtttagtgga
    52321 gaggttgtga aaaagctgtt tagtttcgag tattaagaaa aattttgtgg aaatggcttt
    52381 ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttaag
    52441 gaagaggttg tgaaatcagt gccttgacct gagtattaga gaaacaaaac ggaaaaatag
    52501 cttctcatat tttaccgttt ttttagctaa tatcgagggt cagctgattg aacaccgttt
    52561 atatcaagag gttgtgcgcc tgccgttcag ctccgagtca caaggagtaa tttcaaaaaa
    52621 cagtttttca tgtttgatga ctattgtgac ttgtgaccga ggagttggcc ggagagcacc
    52681 gtttattcta aaaaagaact gcgaactctc gcagttcttt ttttaaaata cgggataaag
    52741 gaagtttcca gcagacaaga gaagaaagca aaagaaattg ctatttgatt cacaaggttc
    52801 tataacgaaa tcgcaatcaa tcaaacattt acaaagaagg taattcaaat ggaaatctga
    52861 tttgatgatg cttctgctgt tgaaataaag cagcatctag cacctgaaaa caaactaata
    52921 gtaacattcg aagatggagt aggtccgtat tttcagcatg caataatcca tatgtaagtt
    52981 caattcaccc ttgaaatcat cggaccagat aaagttatga ccaatctatc ccaagcaatc
    53041 tagaagagat attagtaaag ggacatttac tagaagtctt agatgagcct ataaatatat
    53101 gttctgatgg cgtcatcagt ggtctgattg acggaagctt aggtgtgatc gatctctctg
    53161 gaccaaacaa cgaaggaatc aaggggaatc cggcacgtta agaaaaaaat agcataaaaa
    53221 actagacact tgatgagcgt tcattcaatt gtttgaaggg actaggctgg tgtcattttt
    53281 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
    53341 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
    53401 atagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
    53461 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga
    53521 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt
    53581 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
    53641 ctagcgatca ggctatattc ggaactgaga aatctgctca gcaatttgaa tccgtttatc
    53701 gaaaattact aaatgaactt caaacagtgg gaatctgcaa catcatcttg atggagccgt
    53761 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
    53821 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggtatat
    53881 tcaatgcttt agggattgtt tatggttttt cgaaatatac aagagaagat ggggtccacc
    53941 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
    54001 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
    54061 ggcagtaaaa gaagatctac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
    54121 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
    54181 aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg
    54241 attggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
    54301 ggaagaaatc caaaaaaaat ttgcagaata tttgatactg gaagctaaaa aaagaagaat
    54361 aaaatcattt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
    54421 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
    54481 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
    54541 aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
    54601 atgtcattga ttgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
    54661 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
    54721 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaagaga
    54781 ataataaagg ttagataaag catagagtca ggtgggatac atgtatttca cctgactcta
    54841 tgcgttctcc tgtataagtt cttccatgtg acttttacgt cttttattta tatggggtta
    54901 ctgtttatgg aaagttgaaa taattttcat taaagaactc tttctatatt tttgaaagcg
    54961 tagtggagct tcgaatatct gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc
    55021 tatcgaaaaa ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac ttagttgcta
    55081 tcctgtcaag atcaagaagg caagtgcggg ttggataaga gcagtggcaa tgatcgaaaa
    55141 gaaaaagaaa gaataagcca aaacatctgt atggaagaaa ttttccgatg cagatgtttt
    55201 tcattttttc tttcggatac cttgacatat gaataaccgt tcatttataa ttagttatga
    55261 agagatattc atatgttgag gcgaggaggt cattaaaatg aaaagttaca gattagaagg
    55321 tttagattgt gcgaactgtg caatgaaaat cgaaaaagga gttcagaaga tcaatggagt
    55381 caaggaagca acggtaaact tcacttctgg aaaactaacg attgacgcag aggaagatca
    55441 tttggctacg attgaacaag aaataaaaaa agtagtgaag gaattagaac cagatgtcaa
    55501 agtaacagaa atagataagg aaaaagtatc tgaacatgga aatgaaaaag agagaaatac
    55561 gctgttccgg attttatttt cccttgcagg tatcgcttta ttgctattat ttgattttaa
    55621 tgaaccaatt cgcttgatag ggtatttatt gatttatctg ctgataggat atgatgtagt
    55681 gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc
    55741 tgttgctacg attggcgcta tgttgatcgg tgaatatcca gaagccgtag cagtgatgct
    55801 attttatcaa atcggcgagt atttccaagg attggcagtc agtcattcaa gaaaatcgat
    55861 tcgcgaattg atggcgatcc gtccagaaac agctcacgtg cagacgacgg aaggattgat
    55921 gaccgtgaac cctgaagacg tgcagatcgg tcagtttgta cttgtgaagc ctggtgaacg
    55981 tgtaccgtta gatggaacga tcatcgaagg agaatcatta gtcgatacgt cagcacttac
    56041 aggagaatca gtccctaaat ctgtatatgt tggtgaaaca gtattaagtg gatttatcaa
    56101 taaaaataaa ccatttttag ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa
    56161 attattagaa ttagtagaaa atgccagcag taaaaaagca ccagctgaaa atttcattac
    56221 taaattcgcg cgctattaca caccagtcgt tgtaggattg gccgttttat tagctgtttt
    56281 acctccactt gttgtttctg gagcggcgtt ttctgaatgg atctatcgag cattgacatt
    56341 tttagtgatc tcatgtccat gtgcattagt gatctccgta ccccttagtt tctttggcgg
    56401 tatcggagga gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct
    56461 tgcacaaact gagacagtgg tattcgataa aaccggcaca ttaacgaaag gtgacttttc
    56521 gatccagacg atcgatacaa aaatcgatcc aaagatattc atgcaatatg ttgcgagtgc
    56581 agaacagttt tctacacatc cgattgctca atctgtttta gaaggctacg caggtccgct
    56641 tttaccaaca gctaatatcc aagaatttgc aggtgaaggg atcttagctg aagttgatgg
    56701 caaacaagta ttagcaggta atcacaaatt gatggaaaga tttgaaatct cttttccttc
    56761 aagtcaagaa atcggtacat tgctttatct agcgatcgat caaagttaca gtggttatct
    56821 tgttattgca gataccttga aagaagatgc cgtagacgca ttggttcaat taaaaaaggc
    56881 tggtgtgaag aacacagtca tgttgacggg cgattcgaaa aaaattgctg atcatatcgg
    56941 gaagcaagta ggcgtggaca agatatatag cgaattgctt ccagaagata aagtgcagcg
    57001 gttagaggaa atcttacaaa gaaacaataa aaagaccgca tttgtcggag atggaatcaa
    57061 cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca atgggtggat tgggaagcga
    57121 tgctgcaatt gaagcagcag atgtagtcat catgaatgat caaccatcga agattgcaga
    57181 agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa cagaacattg tctttgctat
    57241 tgggataaaa attttggtac ttgccttagg tgcttttggt tttgcatcaa tgggagatgc
    57301 agtatttgca gatgttgggg tgacagtatt agctgttttg aatgccatgc gaagcttgca
    57361 tgtaaaaaat aaacgttgat caatgaagag gacgaatgaa cgtcctcttt ttttatatcc
    57421 ttttcttaat gaaaaatgta aagaaaatag ataggattat aaaataacta atgtttttaa
    57481 ttttgcagaa aattcaaaat agcttgaaat tcaataaggt aaatgaaata aaagaaaata
    57541 acttattttt ttgatggaaa aaatatgaaa aaacgatgtg tattaaataa aagaatacac
    57601 acataaaata acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa
    57661 caaattttta tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt
    57721 tgcgttaatt gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca
    57781 gcaaggagta tcagttgtca cctcttggct attgattttg gccatttatt ttattccata
    57841 cgcattgatc gttggacaat tagggtcagc cttcaaggat agtaaaggag gcgtcagttc
    57901 atgggtcgaa aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg
    57961 ggtcgtccat attccttatt tggcacaaaa gcctcaagca atcctgattg ctggcggttg
    58021 ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat
    58081 ttcacttatt atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa
    58141 agtgattggc ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt
    58201 agctgtaacg gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca
    58261 agtgaaaaca tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt
    58321 atttgcagtt ggaggagcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc
    58381 aaaggaattt cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt
    58441 aggctcagtc gcaatgggta tgctgtttgc tagcgataat attcctaaag acttgatggc
    58501 aaatggggca tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt
    58561 gatgatcatt tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat
    58621 tgatgcacca ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct
    58681 acgtaaacgt aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt
    58741 aggaatcatt attttgttgc cgatctttgg tatcaaagat atgaatgaat tagtaaaatg
    58801 gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata
    58861 tatgatgttg aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc
    58921 aaaaatcggt tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct
    58981 aggaatggtg ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt
    59041 cacgaatatc atcacaccaa ttgtcttgat cttattaggt atgatcttgc cagcaattgc
    59101 tcgtcgagac aaaaacaaag aaatcactga accagaaaca gtggttcagc cttaaaatag
    59161 aaatacttgt tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt
    59221 tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt gtttttcgca gaagctactt
    59281 ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
    59341 ccaaacaaaa atagcccctc atatttttcg ttttgctggg cttattaccg cagatgcaag
    59401 cttttgaaca ccgtttaaga aagaggtagt gagcctgccg tttaactccg agtcacaagg
    59461 agcaatttca aaaaacaact tctcatgttt gttgactatt gtgccttgtg accgaggtgt
    59521 tggcaggaga acaccgttta aggaagaggt tgtaaaatca gcgatttgac ctgagtatta
    59581 gaggaacaaa cgaaaaaata gctcctcata ttttgccgtt ttttgagtta atgctgaagg
    59641 tcagctgatt gaacaccatt tattcgaaac aagggacttt gacacgactt ttgtcacagt
    59701 cccttgtttt tttgcggcgt tataagtggt atgatcgaac caaattccaa agagaagtga
    59761 gggagaagaa tgaagatcat cgtgattgga caaagtgggt cgggaaaatc gaccctcgca
    59821 aggaagatca aagaaatcac cgactttccg ctactgccgc tagacctttt atggcataca
    59881 acagactaca gtattcaggc aaaaaagtgg tttttgcaag aacagcaatc gtttatggta
    59941 tctaatccgt catggattgt tgaaggaaat tatacttcta cattagagga aaggattaaa
    60001 gaagcagata agatcatttg gcttaaagtg ccgcgttatc tcgccatcta ccgagtagtt
    60061 tggcgttctc taaaaagaaa gataaacaaa aaatctcggc cagatatgcc tgaatcattt
    60121 tcagagaaac taaacaggga atacttagaa tttttatctt ttatctggca ttttgaagaa
    60181 aacaatgaac cgaaaataca gcagctgatc caagtagcta atgcaagaaa taaactgatc
    60241 atccttaaaa caagacgaga gaaacaagaa tttttaacta aattgaaaat agagagcaaa
    60301 aataattgac agtttttgtt tacaagcgta atctgtattc ataagctaat gattacattt
    60361 gtaaacgaaa ggtgtgaaat aaatggaaaa agtcaaaacg ccagtaaaga tcagtgattc
    60421 agagtgggag atcatgcgtg ttatttggac acttggacag acgacagccc aagaaatcac
    60481 gcagatctta gctgattcga tggattggaa accagcaacg atcaaaactt tgctgggacg
    60541 tttggtaaaa aaagaagtga tctggacaga acaagaagga aaaaaattca tttatcatcc
    60601 agctgtctcg gagatggaaa cagttcgcag tgcgacggaa aatctttttt cccatatctg
    60661 tgcaaaaagg attggagaga cgattgcgga tctagtagaa gaagccactt tgactcaaga
    60721 agatatcaat gagattttga aacaactgga acaaaagcaa ccagtagata cgatcgaatg
    60781 taactgtatc ccaggtcaat gtgaatgtaa acaacatcaa taggaggaag aaaaaatgaa
    60841 acaacaattt tcaatcgaag gtatgagctg caatcattgt gcagcaagag tagaagaaac
    60901 agtaagcgca cttgacggcg tgcaaaaagt gaaagtaaat ttgaaaaaag caaacggtac
    60961 ggtcaagttt gatgaaacaa aagttcaatc cgaaaagatt tgtcaagcaa tcaatggatt
    61021 gggttataaa gcagaggtga tttgatggca acagcagaaa ccaaaatgga tacgttcgtc
    61081 attacaggta tgacttgtgc aaattgttct gctaggatcg aaaaagagct gaacggacaa
    61141 cctggtgtgg tcaatgccac agtcaacttg gccactgaaa aagcaagtgt caaatatgaa
    61201 gggacgacca ctgaaaaatt aatccaaagc gtagaaaaca tcggttatgg tgcaattttg
    61261 tacgatgaag cccacaaaca aaaaattgca gaagaaaagc aagcgtattt gaaaaaaatg
    61321 ctattcgatc ttatattgag tacggttttg actttgccgt tgatgttgtc aatgattgct
    61381 atgatgttag gaagccatgc ggcgattgtt cacttttttc attttccaat cgtccagtta
    61441 gtgctgtctg ctcctgtcca attttatgtc ggtgctcgtt tttataaagg tgcgtaccat
    61501 gcaatcaaga cgaaagcacc aaatatggat gtattggtag caattggaac atcggctgcc
    61561 tttgcattga gcatttacaa cggatttttc cgtggacacc cccaagatct ttattttgaa
    61621 agcagcagta tgatcatcac gctgattttg ctcggcaaat atttggaaca tacagcaaaa
    61681 acgaaaacag gtaatgcaat caaacaattg atgtctcttc aaacaaaaac cgcccaagtg
    61741 ataagaaacg ggaaagaaga aacgttagcg attgaagaag tagtcgttgg tgatcaacta
    61801 gtcatccgtc ctggtgagca gattccagca gatggtcgaa ttatttcagg aagcagtgcg
    61861 atagatgaaa gtatgcttac tggtgaaaat cttcctgtag aaaagaatcc ggatgacacg
    61921 ctttttggcg ggacaatcaa tacgaatggt ctgctacaca tggaagtcac acaagtcggc
    61981 aagcagacag tattagcgca aatcattcaa atggtggaag atgctcaagg gagcaaagcc
    62041 ccaatccaaa aaatcgcaga tcggatctca gggatttttg ttccgatcgt attagtcatc
    62101 gcgtttatca cgctcatagc tacaggtttg atcactggtg attggcaact cgcattgatc
    62161 catagtgtct ctgtattagt cattgcgtgt ccatgtgcat taggtttagc gaccccaaca
    62221 gctattatgg taggaacggg tgttggtgca cgaaatggaa ttttaattaa aggcggagaa
    62281 gcattagaag cagctgctca cttagacagc attgttttgg ataaaactgg aacgattaca
    62341 gaaggaaagc ccaaagttac agatctagtc ggttcaaaag aagtcctatc tattttttat
    62401 actttagaac aagcttctga acatccacta ggtaaagcaa tcgtggaata cggaaaactg
    62461 caagaagcag caacatacga tatgattgat tttacagccc acccaggtgc tgggatcagc
    62521 ggtacgatta atggagtacg ctattttgcg ggtacacgta aacgattaat tgaattgaac
    62581 ctatcatttg atgaatacca agaacacgcg ttagaattag aacagcaggg aaaaacagtc
    62641 atgtttttag ctgatgaaaa gcaagtgatc gggtggattg ccgtggctga tcaaatcaaa
    62701 ctagaagcca aacaagcaat caagcagctc cagaataaag gattagatgt ttttatgctg
    62761 acaggagaca acaaactggc agccgaaacg attggtaaac aagttggtat tgatccaaag
    62821 catatctttg ctgaagtact tccagaggat aaagcagcct acgtagaaaa acttcagaaa
    62881 gatggcaaaa aagtcggtat ggcaggcgat ggaatcaatg atgcaccggc cttagcatta
    62941 gctgatgtcg gtatggcaat gggaagtgga acggacattg cgatggaaac agccgatgtg
    63001 acgctgatga atagcagttt ggcatccatt gcacaaacaa tcgagttgtc taggttgaca
    63061 ttgagaaaaa tcaaacaaaa tcttttctgg gcttttgtct ataatacgat tggtattcct
    63121 tttgccgcac taggcttttt gaatccaatc attgcaggag gagcgatggc gtttagttcc
    63181 gtcagtgtat tgttgaactc cctgagctta aatcgacaca tgaaaaagta agagaaggga
    63241 tggagtaaaa atgaatggga acgatatgga gaaaaaacat gagcataaaa aagaagtagt
    63301 aaacaaagaa agtacagaat ctcatgaaaa acagatggag atgaaacatg accatagtca
    63361 gatggatcat tccatgcaca tgggacataa ccatggagat atagcaaaca gcaaacaaat
    63421 ggaccattcg atgcacatgg atcacgaaca cggcggcatg gatcattcca tgcacatggg
    63481 gaatttcaaa caaaaatttt ggctatcgct tatcctagct atcccaatca ttcttttttc
    63541 accaatgatg ggaatggaat ttccatttca agtaacgttt cctggttctg attggctcgt
    63601 attgatctta gcaactatac tatttatcta tggcggtcag ccatttttaa gtggagctaa
    63661 gatggaattg aaacaaaaaa gtcctgctat gatgacactg atcgcgatgg ggattacagt
    63721 tgcatatatt tatagtgtct attcgtttat cgcaaatctg ctcaatccac atacacacgt
    63781 catggatttc ttctgggaat tggctacttt gatcgttatt atgctactag gtcactggat
    63841 tgaaatgaat gctgtgtcga atgccagcaa tgctttacaa aaactagcag aattactgcc
    63901 agaatcagtg aaacgcttga cgaaagatgg aaaagaagag acagtttcat tgaaagaagt
    63961 aaacgaaggt gatcgtttga tcgtccggtc tggtgataaa atgccaacag atggtgtaat
    64021 cttgaaaggt gaaacaattg ttgacgaatc agctgtgact ggcgaatcaa gaggaatcaa
    64081 aaaacaagcc aatgacaaag tgattggcgg ttctatcaat ggggatggaa cgatcgaaat
    64141 cgaagtaact ggaactggtg aaaatggtta tctagcgaag gttatggaaa tggtgcgaaa
    64201 agcacaagga gaaaaatcca agctagagtc attatctgac aaagtggcaa aatggctgtt
    64261 ttatgtcgca ttaattgtgg gtatcctggc attcatcgcc tggttgttct tgacagatct
    64321 tcctaatgca ttagaacgta tggtaacagt ctttatcatc gcctgtcctc atgctctagg
    64381 tttagctatc ccattagttg ttgctcgctc cacatcaata gcagctaaaa acggattact
    64441 tttgaaaaac cgcaatgcac tggagcaagc caatgatcta gatgtgatca tgctggacaa
    64501 aacaggaaca ctaacagaag gaaaatttac tgtaacgggt gtcgaagtac ttgatgatgc
    64561 gttcaataaa aatgaaatcc ttcaatatct aggagccttg gaagcgaatg ccaatcatcc
    64621 attggctgtt ggcattatga attatttgaa agaacatgaa atcaagcctt atcaagctga
    64681 aaatctaaaa aatctttcag gagtcgggct agaggcaaca gtcaaaaaac aacaagtgaa
    64741 aatagttaat gaaaaagaag ttgagcgtct cggattaaac gtagaacaag cattattgaa
    64801 accttaccaa gaacaaggaa ataccatcag cttcctgatt ttagaaaatc atctagctgc
    64861 aatcgttgct ttaggtgacg tagtaaaaac agaagcaaaa gaatttatcc gaacattgaa
    64921 agaaagaaaa atcactcctg tcatgcttac tggtgacaat aaaaacgctg cccaagcagt
    64981 agcagattat ttagggatcg aagaatatta tggcggttta ttaccagatg ataaagaagc
    65041 tgttgtccag aaataccttg accaagggaa aaaagtaatc atggtgggtg atgggatcaa
    65101 tgatgcgcca agtttagccc gtgcttcaat cgggatggca atcggtgcag ggacagatat
    65161 cgcaatcgat tctgcggatg tcgtattgac aaacagcgat ccaaaagata ttttgcattt
    65221 tcttgatcta gccaagcaaa cgcgaaagaa aatgatccag aacctctggt ggggggcagg
    65281 ttacaatatt cttgcaattc cgctagcagc cggaattctt gcgccaattg gtatcatatt
    65341 aagtccagca gttggtgctg tcttgatgtc gttaagtacg gtagttgtag ctctaaatgc
    65401 tttgacattg aaaatcaaat aataatgaaa aaag
//
LOCUS       DACIWG010000010        61534 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.010, whole genome shotgun sequence.
ACCESSION   DACIWG010000010 DACIWG010000000
VERSION     DACIWG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.010"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(60..188)
                     /locus_tag="IYF54_000617"
     CDS             complement(60..188)
                     /locus_tag="IYF54_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ9830974.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="IYF54_000618"
     CDS             complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="IYF54_000618"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ9830975.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1675..2730)
                     /locus_tag="IYF54_000619"
     CDS             complement(1675..2730)
                     /locus_tag="IYF54_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ9830976.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="IYF54_000620"
     CDS             complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="IYF54_000620"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ9830977.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="IYF54_000621"
     CDS             complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="IYF54_000621"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ9830978.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="IYF54_000622"
     CDS             complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="IYF54_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ9830979.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="IYF54_000623"
     CDS             complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="IYF54_000623"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ9830980.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="IYF54_000624"
     CDS             complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="IYF54_000624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ9830981.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10492..12051
                     /locus_tag="IYF54_000625"
     CDS             10492..12051
                     /locus_tag="IYF54_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ9830982.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12162..14093)
                     /locus_tag="IYF54_000626"
     CDS             complement(12162..14093)
                     /locus_tag="IYF54_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9830983.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="IYF54_000627"
     CDS             complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="IYF54_000627"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9830984.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15022..15774)
                     /locus_tag="IYF54_000628"
     CDS             complement(15022..15774)
                     /locus_tag="IYF54_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9830985.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="IYF54_000629"
     CDS             complement(15933..16142)
                     /locus_tag="IYF54_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830986.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="IYF54_000630"
     CDS             complement(16153..17481)
                     /locus_tag="IYF54_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830987.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17846..18433)
                     /locus_tag="IYF54_000631"
     CDS             complement(17846..18433)
                     /locus_tag="IYF54_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ9830988.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IYF54_000632"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IYF54_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ9830989.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /locus_tag="IYF54_000633"
     CDS             complement(20013..21302)
                     /locus_tag="IYF54_000633"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ9830990.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="IYF54_000634"
     CDS             complement(21456..22388)
                     /locus_tag="IYF54_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830991.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22545..23204)
                     /locus_tag="IYF54_000635"
     CDS             complement(22545..23204)
                     /locus_tag="IYF54_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ9830992.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="IYF54_000636"
     CDS             complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="IYF54_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ9830993.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24245..24535)
                     /locus_tag="IYF54_000637"
     CDS             complement(24245..24535)
                     /locus_tag="IYF54_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830994.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24945..26750
                     /gene="glmS"
                     /locus_tag="IYF54_000638"
     CDS             24945..26750
                     /gene="glmS"
                     /locus_tag="IYF54_000638"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ9830995.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26822..28177)
                     /locus_tag="IYF54_000639"
     CDS             complement(26822..28177)
                     /locus_tag="IYF54_000639"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ9830996.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28201..29370)
                     /locus_tag="IYF54_000640"
     CDS             complement(28201..29370)
                     /locus_tag="IYF54_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9830997.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29367..30254)
                     /locus_tag="IYF54_000641"
     CDS             complement(29367..30254)
                     /locus_tag="IYF54_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ9830998.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30443..34123)
                     /gene="nifJ"
                     /locus_tag="IYF54_000642"
     CDS             complement(30443..34123)
                     /gene="nifJ"
                     /locus_tag="IYF54_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ9830999.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34321..34842
                     /locus_tag="IYF54_000643"
     CDS             34321..34842
                     /locus_tag="IYF54_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ9831000.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35541..36106)
                     /locus_tag="IYF54_000644"
                     /pseudo
     CDS             complement(35541..36106)
                     /locus_tag="IYF54_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36285..36941)
                     /locus_tag="IYF54_000645"
     CDS             complement(36285..36941)
                     /locus_tag="IYF54_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831001.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37509..38444)
                     /gene="whiA"
                     /locus_tag="IYF54_000646"
     CDS             complement(37509..38444)
                     /gene="whiA"
                     /locus_tag="IYF54_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ9831002.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38478..39476)
                     /locus_tag="IYF54_000647"
     CDS             complement(38478..39476)
                     /locus_tag="IYF54_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ9831003.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39473..40357)
                     /gene="rapZ"
                     /locus_tag="IYF54_000648"
     CDS             complement(39473..40357)
                     /gene="rapZ"
                     /locus_tag="IYF54_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ9831004.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40492..41223)
                     /locus_tag="IYF54_000649"
     CDS             complement(40492..41223)
                     /locus_tag="IYF54_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ9831005.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41227..42492)
                     /locus_tag="IYF54_000650"
     CDS             complement(41227..42492)
                     /locus_tag="IYF54_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ9831006.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42755..45571)
                     /gene="uvrA"
                     /locus_tag="IYF54_000651"
     CDS             complement(42755..45571)
                     /gene="uvrA"
                     /locus_tag="IYF54_000651"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ9831007.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45581..47575)
                     /gene="uvrB"
                     /locus_tag="IYF54_000652"
     CDS             complement(45581..47575)
                     /gene="uvrB"
                     /locus_tag="IYF54_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ9831008.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47834..49336
                     /locus_tag="IYF54_000653"
     CDS             47834..49336
                     /locus_tag="IYF54_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9831009.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49338..50075
                     /locus_tag="IYF54_000654"
     CDS             49338..50075
                     /locus_tag="IYF54_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831010.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50443..51609)
                     /gene="dnaJ"
                     /locus_tag="IYF54_000655"
     CDS             complement(50443..51609)
                     /gene="dnaJ"
                     /locus_tag="IYF54_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ9831011.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51760..53589)
                     /gene="dnaK"
                     /locus_tag="IYF54_000656"
     CDS             complement(51760..53589)
                     /gene="dnaK"
                     /locus_tag="IYF54_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ9831012.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53640..54203)
                     /gene="grpE"
                     /locus_tag="IYF54_000657"
     CDS             complement(53640..54203)
                     /gene="grpE"
                     /locus_tag="IYF54_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ9831013.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54297..55340)
                     /gene="hrcA"
                     /locus_tag="IYF54_000658"
     CDS             complement(54297..55340)
                     /gene="hrcA"
                     /locus_tag="IYF54_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ9831014.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55575..56741)
                     /locus_tag="IYF54_000659"
     CDS             complement(55575..56741)
                     /locus_tag="IYF54_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ9831015.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56757..56984)
                     /locus_tag="IYF54_000660"
     CDS             complement(56757..56984)
                     /locus_tag="IYF54_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ9831016.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57371..57627)
                     /locus_tag="IYF54_000661"
                     /pseudo
     CDS             complement(57371..57627)
                     /locus_tag="IYF54_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57970..58993)
                     /locus_tag="IYF54_000662"
                     /pseudo
     CDS             complement(57970..58993)
                     /locus_tag="IYF54_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59631..60305)
                     /locus_tag="IYF54_000663"
                     /pseudo
     CDS             complement(59631..60305)
                     /locus_tag="IYF54_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60828..61418
                     /locus_tag="IYF54_000664"
     CDS             60828..61418
                     /locus_tag="IYF54_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ9831017.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17075 a        13214 c        10645 g        20600 t
ORIGIN      
        1 ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct ttagcacgtc
       61 tatttattaa agattttagg gccttttctc atgtttttac gatcattctt aggtgtttcc
      121 atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat tttttctttt
      181 tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga tgggtttgcg
      241 ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca aataacttag
      301 catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct ggtgacattt
      361 cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca actacgatca
      421 cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct tttccgactt
      481 cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct tcaattgctt
      541 ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac aagccaatcg
      601 tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg gcaaactcga
      661 tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat gactttgctg
      721 agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca tataaaactt
      781 tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg atgaccgaaa
      841 ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta aagaattttt
      901 ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga cggataatga
      961 tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg aaccgttgca
     1021 agatatctct gactactttg attgcttgat cgatgacagg atcttggata taaaaatctt
     1081 cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac tgtccatcga
     1141 tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc attccgaccg
     1201 ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc tctacttgtt
     1261 tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg atacctatgc
     1321 gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata aatgtttcga
     1381 cttttccata tccaaactta ttgcctgctt ttactactag tatttctact tcttctccag
     1441 ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc attccttcat
     1501 aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt tgctgttctg
     1561 tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta taaatcagct
     1621 aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt ttgattaatt
     1681 cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt catccgttaa
     1741 acgttcgact tcttttacga attctttgct gacaacctct aattcttctt catctgtacc
     1801 tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt gttgatgaag
     1861 attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga tcattactgg
     1921 tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat agattattct
     1981 tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca agatctcaaa
     2041 gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt tggcatctgg
     2101 cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca tcgaagcatt
     2161 tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta ctcgagggag
     2221 catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta gttctgatgg
     2281 aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga agtaactgtt
     2341 atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt ctgctgctgc
     2401 tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg taccagctgg
     2461 gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca cttcgttgat
     2521 tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga ctctacatgc
     2581 ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt caaatcctgc
     2641 ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt ttcccgaggt
     2701 ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg ctaattcatc
     2761 tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt tcatgatttg
     2821 accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga cagattgttg
     2881 gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag ataattgcac
     2941 ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca attcacggaa
     3001 gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa tcattcctgc
     3061 taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt tcaagtaagc
     3121 agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat tttccaaaac
     3181 agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt catactctgg
     3241 aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag gcaaactagc
     3301 ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag gctctgggaa
     3361 gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat tcgttgtttc
     3421 gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt ttgcttgacg
     3481 tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt ttttcaattc
     3541 tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg catcacagcg
     3601 catagagcct tcttccattt tcacatcgct gacttctgta aattggataa ttgatcgcaa
     3661 tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag aaacgatttc
     3721 gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa taccatgcat
     3781 gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt tctttttacc
     3841 ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt gggaaatttg
     3901 atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag tatctttaga
     3961 gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag ctttattcat
     4021 tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt ttggttctgc
     4081 accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact cgacgtggac
     4141 ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat agctggttgt
     4201 tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat cgttttttca
     4261 tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa acctgccggt
     4321 acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc actcatgtac
     4381 attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg tgaagatggc
     4441 ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat caatgtacgg
     4501 acttgtcccg cttttttgaa gtaagcgtca taataaccag cacttaagga gaatgtacct
     4561 aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt gacatataca
     4621 tcatctaatg atttcacatt ttctgagcga taaccgtagc ggataccatc gaatcgttgc
     4681 aagttggaac ttgcttctga tgaggcaatg atataataaa cagccacacc gtattttgaa
     4741 tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc tgctgctttc
     4801 aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt aggtaaagca
     4861 atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac tgaaacgcct
     4921 gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa tgcattatct
     4981 ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc aatcaaacca
     5041 aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa agctgctggt
     5101 tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc agctgcaact
     5161 gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca agcatttttt
     5221 gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc catgtttagc
     5281 ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc atcataaatt
     5341 ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt tgttacgata
     5401 ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat ccctttttca
     5461 tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa tgcttcgacc
     5521 tcaggctctg tttctttgat tcgttcaaat gttgcttttg tcaaatcttg tacagtgatt
     5581 tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa ttctgtcatt
     5641 atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg tcttctgttt
     5701 ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga tcttgtcgca
     5761 tcacgtttac tgtttcaacc acattcgacg taaatggaac accttctgta tctacttctt
     5821 ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt gcaagttctt
     5881 catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct tcactaatcg
     5941 ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt aacacaagag
     6001 tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt cattcttcta
     6061 ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact tcgattccta
     6121 attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg atatccgttt
     6181 ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct tttgcttctt
     6241 cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta aaaggagact
     6301 cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg cctgcttgtt
     6361 ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact acactgtcgg
     6421 caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt ttactcaatg
     6481 tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg acatgtcgga
     6541 ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg attgctgtca
     6601 aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct tcttctgtca
     6661 atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt tctagtacgc
     6721 gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc aaaccttctt
     6781 tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca tccaaatgga
     6841 caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg ctgtctttag
     6901 gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct ttataaatca
     6961 gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta tgcaggctgg
     7021 cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt ggcgttacta
     7081 cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact tcttctggcg
     7141 gaaacttgta agctgctgcc catctcggtg ctttgacagt aaaaccaagt tgatcttgta
     7201 gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa tccacacgtt
     7261 tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat agacgttttt
     7321 cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac tgggtttttg
     7381 cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt cttttcgctg
     7441 tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga ttcgcaaaga
     7501 tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt tttggcatga
     7561 aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg accgaccgga
     7621 cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga gtcgcacctc
     7681 tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt tttagttcac
     7741 agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa gctatcagct
     7801 cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg actttttcaa
     7861 aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg atcaaatcag
     7921 gatattgttc ctcaattgct actaattctg cgtattcttt atcataaaca tagtcttcca
     7981 ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt gtacgaagtt
     8041 cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt taccctccac
     8101 tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc ttgacttggg
     8161 aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac tgttcctaga
     8221 ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc ccctccagta
     8281 gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc tgttttttta
     8341 gcgaatacag gtgttttttt aggcgtagga cgcatgcctt cgatttccag taactcctga
     8401 tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata taatgttctg
     8461 gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac atatgcgaga
     8521 cggcgttctt cttccagttc gctttcttcc atcaatgctc gagataaagg aaaaaccccc
     8581 tcttctagac caatcaggaa tacaactggg aattctaatc ctttggctgc atgcaaagtc
     8641 atcaatgtca cttgggaagc atcttcttct aaattgtcaa tatcggaaac aagtgccaga
     8701 tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc attttgctgt
     8761 tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt ttcgatgcgt
     8821 gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct acgatccaaa
     8881 acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac ttcttggatc
     8941 atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc cagatccaca
     9001 ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg tagtttttcg
     9061 atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa actgattgaa
     9121 tcttgaggat ttgccagcac attcaaatac gctaaaatat ctttgatttc tttacgatcg
     9181 tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag agtttcttcc
     9241 attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta attccgatga
     9301 ttgcttcgaa tctcttcttg catccgatct acgataaaac gggtttcgtc tcgttcatta
     9361 tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa gttttttggt
     9421 tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt tttcgtggag
     9481 cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc aaaatccaag
     9541 atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc tcccacgacg
     9601 cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc gtgatttgta
     9661 tcttggtact catctacatg aatataatgg aatttgtttt gataatatgt gagtgaatca
     9721 ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc catacattga
     9781 ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc ttcgtaataa
     9841 ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc ttgagagatt
     9901 gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc ttttagtatc
     9961 cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg gttatacccg
    10021 ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt cgatacccag
    10081 acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc ttttgcagcc
    10141 ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc ctcaatcaaa
    10201 taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc cattagcagt
    10261 aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc gtttaataat
    10321 tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa ttatagcatt
    10381 ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta tggatttttc
    10441 cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg aatggaaaaa
    10501 aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa aggaatattt
    10561 gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga aaagctaagc
    10621 gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt aattggccta
    10681 gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa tgaaccggat
    10741 atcaagggaa gcgggattcc acaagtagag ggacagcttc gcggggaatt atcaatgaac
    10801 tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg tgcgggcctt
    10861 tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca aggagctgga
    10921 caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg tgcaagtagc
    10981 ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt agaagaaatc
    11041 catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat cactgctaac
    11101 ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga ccttccttct
    11161 ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg catcttaggt
    11221 tttatttatc agaaggtctt gctggctttg ccaaaatttt acaaccgatt gcctttacca
    11281 gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta tttatggcct
    11341 agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc cttgccaatc
    11401 attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc ttacggtggt
    11461 ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat cggtagcttt
    11521 tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga ttttgtcgtt
    11581 attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc aattatttta
    11641 gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc tgctcttgtt
    11701 gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt attggaacgt
    11761 ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt tgattttcca
    11821 gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg gcctaaaaat
    11881 atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg tgatacgatc
    11941 atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc aagaatcaag
    12001 caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta acgaacatgg
    12061 aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga ttctataaaa
    12121 aaagaagaga ctgggaaaaa ccagcctctt cttttttatt tctatgcttc tgctgctgca
    12181 ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat agcaataaac
    12241 accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc atgaggagcc
    12301 agtaatttga tcccgaaagc tccgactaat ccgccagtta aagcagaacc aataataaaa
    12361 cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc cgtgacaaac
    12421 gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct gaatttattc
    12481 ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc agccataaca
    12541 gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc tgtaccaaac
    12601 acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc agctaataat
    12661 gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt caatgcatca
    12721 ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat cagtaagcca
    12781 aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa gttcttgaat
    12841 aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag tgcacctaag
    12901 aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat caaaccaggg
    12961 cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa accaaatgca
    13021 gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag atttgataat
    13081 tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat agcgattcct
    13141 ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt gtaaattctt
    13201 tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc atcaccatgg
    13261 aatactggtg cagtaccact aagggctaga ttgatcagct cttctggttt acggatacca
    13321 tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc tacttttttg
    13381 tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa tcggtttttg
    13441 attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt cgcttttttc
    13501 ttcagagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc tgtaacggca
    13561 acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc ttgttctgct
    13621 ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac ttcttctggt
    13681 gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg agatagtgct
    13741 gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat gaaaaacaga
    13801 taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt tgcgaataaa
    13861 actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc atcaccaaga
    13921 cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc gatatcggaa
    13981 atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt tttatcagtc
    14041 gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt catgattatc
    14101 ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca tcaatcaatt
    14161 cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact ccccatttaa
    14221 aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccggca atcattgaat
    14281 cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta taagttcctt
    14341 gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc tgtgcccctt
    14401 cagctagcaa ttttttacca tacggaatga tttcttctac agattggaag gttgtattgt
    14461 aaagctcagc taattcgtga ttgtttggtt tgatcaacaa tggtttctta acaagtgcat
    14521 tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct cgaatgatat
    14581 caatcagttc ttgataaaat ccttttcgca ggctagccgg cgcgcttcct gacaatacga
    14641 cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt tcttcatctg
    14701 aaatagatgg gcccaaaccg ttaatttcag tttcagtatc tgattttagt ttgatattga
    14761 ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt tcaagccact
    14821 tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat tgagaatcga
    14881 tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg aagtcttcct
    14941 tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa tcaattgatg
    15001 gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct tttcacggaa
    15061 ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt ccaaaggtgc
    15121 aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa atgtctcttc
    15181 ggaatttttc atggctaaac gtttaacagc tgcctcttct gggtccggag tcgtgaatcc
    15241 ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa aattttttag
    15301 ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg ttccacctaa
    15361 aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg aatttgtcac
    15421 tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag agccagcgtc
    15481 taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga tttgctgttt
    15541 ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca ataattgctc
    15601 tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct cttgtaaatc
    15661 acgtcttatc gttgattcag aagcattaaa aaggtttaca agatcttgtg atttcacgat
    15721 tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt tcctctgtaa gcattttcac
    15781 gatcacctca atacaaataa tagcatattt tttttctact cgcaatataa atcagtcaaa
    15841 aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca aaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgcg agcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tgaatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttaaaat ctttttataa tttaattata
    17521 accaacgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc ctccctgagg tggcacgctt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcacgttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcatt taacacggaa
    21661 ctaagcagcg catcttcatt accgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc tactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat gttcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    22501 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    22561 tggttgaatg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgcct
    22621 agatgttgct ttttctgatt aaagattgct ggaagatacc ctaacatcag caaaggaaat
    22681 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    22741 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg accatgattg
    22801 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagata
    22861 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    22921 cgcgtaatag ctccgataca caaaagatcg accaagaaag caaacattcg gataaaaaaa
    22981 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    23041 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    23101 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    23161 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    23221 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    23281 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    23341 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    23401 cctttcgcat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    23461 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    23521 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    23581 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    23641 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    23701 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    23761 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    23821 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    23881 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    23941 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    24001 aacctgaagc ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    24061 tactgtctcc ctcttccaac acatttgaag ctagttcttc atccccatat aaccaactgt
    24121 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    24181 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tctcttattc attctttctc
    24241 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    24301 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgtcaat tcatcttcta
    24361 ccgtaaactc acgagcttca gcaacttcca aggcatcagg tgcgatttct tggatgctat
    24421 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    24481 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    24541 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    24601 aacccattat tttttgacat aaaaagagca tttgacaaag catttgatat ataccaattt
    24661 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    24721 ccagataaaa aacaaaagag gaggatagtt atacctttta ggatagcgcc aggtctgtgt
    24781 aaacagatga cgaggagagg gtttatcgaa agattcggcg gatggcccta gggactgcac
    24841 tctacagaaa atccacaaaa aatatctgca aagataaaac aaaaggattt tcctgcagct
    24901 ggaagcattc atacgaattc attatagaaa gtaggatgtt ttttatgtgc ggaattgttg
    24961 gaatgattgg attaaaaaat gtaacaccag gattgattga cggtctagaa aaattagaat
    25021 accgtggtta tgattcagct ggtatctttg tctcagatgg cacaactgat tatctggtca
    25081 aagcacaagg cagaattcaa aatttgaaaa acaaaataaa tattgatact acaggaaata
    25141 taggtattgg tcatacacgc tgggcaactc acgggcaacc ttctgaagaa aacgcccatc
    25201 cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa tggcgtaatc gaaaactttg
    25261 aagaacttaa aattgcttat ttagcaaatg accactttat tggtgagacc gatactgaga
    25321 ttatcgctca tctaattgaa actttcgcga aggatactac aacgcaagag gcgtttttaa
    25381 aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg acagcacctg
    25441 atgttattta cgtcgctaaa aataaaagtc cactattaat cggactcggc gatggattca
    25501 atgtgattgc cagcgatgca atggctatgc ttgctcatac gaaggaattc gttgagattg
    25561 aagatgaaga gatggttacc gtcacatcag aaaaagttat tattcaaaat tttgcaggtg
    25621 acatcgtaga acgttcttca ttcgaagcac aagtagattc ttcagatatc gagaaaggta
    25681 cctatccttt ttatatgctg aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg
    25741 ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg gtcgatacat
    25801 tgtctgatag cgatcgaatc tatatcgttg cttgcggaac tagttaccat gcggggttag
    25861 caggtaaaca gactctggaa aaattgacac agatccctgt tgaggttcat ttagccagtg
    25921 aatttggcta taacacacct cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa
    25981 gtggcgaaac agcagatagc cgccaagttc tggtaaaaat caaccgtctc ggctaccctt
    26041 cacttacgat tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc tttacactgc
    26101 tattgcatgc tggacccgaa atcgcagtag catcaacgaa agcttatact gcacaaatag
    26161 ctgtactaac tttattagcc aaagctatcg gtgataaaaa agaatttgaa acttctcttg
    26221 tattcgatat cactcatgaa ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc
    26281 aaaaagatta tttagaagag ttggcagaag tatatctcag cgatacaaga aatgcctttt
    26341 acattggacg tggcgctgac tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa
    26401 tcagttatat ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga acgattgctt
    26461 tgatcgaaga aggaacacca gtgattggaa tcatcagtga agaagtgact ggtgcgcata
    26521 ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt attgtctcag
    26581 agggattaga aaaggaaagt gaccagctgg tgctaccaac tgtccaccct tatctaacta
    26641 ctctaaccac agtcgtaccg actcaattac ttgcatatta cgcaacgcta ttacggggat
    26701 tcgacgttga caaaccacgt aatttagcga aatcagtgac agtagaataa taaaagaaca
    26761 aggaccagtg tcagacatct gacactggtc cttgttcttt tattttttat gaaaatatct
    26821 cttaatccag tccgatttct tctttgacta ctgccgcgat tttatcgaca tagtaattaa
    26881 ctttctcatc agtaggcgct tctgccatca ctcgaagtaa tggttctgta ccggaaggac
    26941 gaactaggat tcttccttct gatcccattt cagcttctgc ctcttcaatc acttttttga
    27001 ttgccggaac atccatcgca ccgtttttat cacttactcg gatattcact aatttttgcg
    27061 gatagattgt tacctctgct gcaagctctg ataatttctt acctgtctgt ttcatgatat
    27121 tcagtaactg aattcctgaa agcatcccat ctccagttgt gttaaaatct aagaaaatca
    27181 tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt gcgcatttct tctacaacgt
    27241 aacgatcacc aacttgtgta acgacatctt tcaaaccgat tccttccacc gctttgtgga
    27301 atcctaagtt actcatgaca gtcgtaacga tcgtatcttt tttcaatcga tttttttctg
    27361 ccaagtattt agcacagata aacatgattt tatcaccgtc gacaatgttt ccaagttcat
    27421 ccaccgcaat aatacgatcg ccatcgccat cgaaagctag tccagcatct gctccttttt
    27481 caacgaccat ctcagctaaa cgttctggat gggtagagcc tacgccatca ttgatattta
    27541 acccgtctgg atttgtgccc atggtataaa aatctgtttc aagatcggca aacaaacgat
    27601 taacacttgt tgaagttgct ccgtttgctg catcgacaca aatggtcaaa ccggaaaggt
    27661 caccgttgat cgtttgtaca aggaattgag aatatttcaa taagccttct ggaaattctt
    27721 ctacagttcc caatccttca gctgatggac gaggcaattc atccacttca gcatctaata
    27781 atgcttcaat ttctagctct tggtcatcta ctagtttaaa accatctgca ccgaaaaatt
    27841 tgattccatt atcttgtgct ggattatgag aagcagaaat catgacccct gcgcttgctt
    27901 tttgcaaacg tgtcagataa gcaacacctg gtgtagagat cacacctagc tggaacactt
    27961 cgatacccac cgataaaagt cctgaaatca gcgcttcttc taacatttga cctgagattc
    28021 ttgtatcccg ccctactaac acgcgtgggc gttttccttc ttcttcatgc tggcttagga
    28081 cataacctcc gcaccgtccc agcttaaatg caagttctgg cgtaagttcc ttgttggctt
    28141 caccgcgtac gccgtctgtt ccaaaatatt tacccatagt tgtaaaatta ctcctttaac
    28201 ttaattttct gaggaatttg ttctgctcat ttgtgttgat tcgcttgttt ctgatgtttc
    28261 ttctgtactt gtggtcgagc tgcttgtccc agattccgaa ctggaagaaa tggttgttgt
    28321 cgatggttcc gttcctgaat cagcagatga aatacttcta ctcgtacttg tattttcttg
    28381 tgtccgactg ctcgtcgaag cagagctact gcttgacgtt tgcaacacgg gtgtaatctc
    28441 cacactaact gattcgggct tgatacttaa cccctcttct actggtattt caattttacg
    28501 agttgttcga tgattgatac ctgagacatt gatcggtaca gcaatactgt caccgatcga
    28561 tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt tttgaaaaac tgaaatcata
    28621 gctttctacc ccggcagctg gtgttccttg ctgtatccca taaagtcgta ccgacttgct
    28681 tggcttgatc gaatcagctc tcactgttac ttgcactgga tcagagacga tacttaatac
    28741 ttcccctgct gcattcagcg cctgtattgg gacctcacta ttaatcccat catctgtgac
    28801 catcgaagcg tccacatcag ctactactcg tttgatttct gccaatgttt tgtcacctgt
    28861 agtgatagag acttttttag gactaacgga agtatcatca agttcatatc cagatggtgt
    28921 gaacgttgaa ggaatcaacg tttccacatc gaaatctttt attacctttt tctcaaccgt
    28981 tactgtaata gtcgatggtt cgatctttgc tgtgacagca ctgctcaaat tttgaatttt
    29041 tagcggaacc tcatgtgttc cttcgcccag cttagtgata tctgctgtga cgcggaacat
    29101 ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg tttgcactgg aaagtttgac
    29161 agtcactgca ttttcatatc catggatata gtagcgcttt gaatcataag aaatcttgat
    29221 tggcacccca gtaactgttt gattatacgc ttcatttcca gaaagcgtat ttggaacgga
    29281 ctggccattt gcattgaagt acagcgcaat actgaacagc aatgcaataa aactgtaaag
    29341 aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc ctcccttagt cataccgtct
    29401 aggaaagatt gaatcacgtt tttcttgttt tttgttttct cttcagcaat cagttctcta
    29461 cgcaaaattg ccaaatattc ttgctgggat aagccagcca tcagttcatt atctaatgta
    29521 atgctgacat ccccagtttc ttcagagaca acgatggtaa tcgcgtcgct gacttcgctt
    29581 ataccgacag ctgcccggtg tcgagtcccg aattcttttg gaatcaacat actttctgat
    29641 agaggaagat aggcgcttgc tactgcgatc tttccttctt ttacaataac tgcaccgtca
    29701 tgcagcggtg tattaggaat aaagatattg atcagcagct ctccagtgat atctgcatct
    29761 aatgcaattc ctgtctcaat atactcatct aatccagtat gccgttcgat agtaattagc
    29821 gcaccgattt ttcgcttgga catatattgg atggctttgt caaaagacaa gatcatcttc
    29881 tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg aactgcgtcc aagatgttcc
    29941 agtcctctcc ggatttccgg ctgaaaaatc acgatagcag ctatcacccc gtacgtaatg
    30001 acttggttca tcagccaaga caaggtgtgc agaccaatga tttccgctac gattcggata
    30061 acaataaaaa tagctacacc tttcagaagc tgaatcgctt ttgtgcctcg cagcagttgg
    30121 atcagtttgt atacaagata ccagacaacc agaatatcaa gtatgttcac caaaaaattc
    30181 agagagaaca agtcaccgga aaacatttgc tgccaatagt ccaaattaaa aagttccccg
    30241 atttgaaatg acataaaaag cctctcttct cttttgagat ttcctctcta gtatatcata
    30301 tgttttaatt ttagaggctg atctgctcgt atttttcttg agaaaaacgc ataattttca
    30361 ggaaaattgc aagtatgcgt tttatttttc tctgcaataa atatttttaa acttcagaga
    30421 attattcatt atattttttc tgttattttt cctttttttc ttttgtagct tgttccattt
    30481 ttttacgtcg ttctgctctg gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt
    30541 cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt ttcttgattt
    30601 tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg tcttggatag
    30661 tcttttcaaa taaagctttt gcaagatttg gatttgcgtt ctctagcgag gaaaagcgaa
    30721 cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag tccaaagtca
    30781 ttgggttctt gtttttttcg accattgcag ggttatatcg atataatgac caatagccag
    30841 aagaaacagc ttcttttgct tctttaagcg attctttcat tccaccaagc aaaccatgtg
    30901 tgatacaagg cgtataggca atgattaaag aaggtccagg atacttttct gcttcttcaa
    30961 aagcctttat cgtctgcatt tgattggcac ctgaagcaat ttgcgccaca taaacatttc
    31021 catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca ctagccgaaa
    31081 atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta ttggaataga
    31141 cttcgttatc caaaacaaga atattcacgt ctgctccgct ggcaagtaca tgatcgatcc
    31201 cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg ctgtttttta
    31261 cgaatagatc ctttctcttg tatatatcct ccagttcagg gacaattgat cgttcgcttt
    31321 cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcgctctca aacatgtgta
    31381 ccaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat acttctttga
    31441 ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca taaccaaatt
    31501 cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat tcattggtcg
    31561 tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc gcattcgcga
    31621 tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt tcgccacagc
    31681 ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac aagacagtat
    31741 tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc caattcacag
    31801 cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt gcgggacaag
    31861 cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata cggtatttta
    31921 cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct tccatttcct
    31981 cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca cattcattac
    32041 acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc cgcttttcca
    32101 tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca agtgtaccaa
    32161 cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt atataaccag
    32221 tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct gggatcgcga
    32281 cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct acgatagcca
    32341 ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc acctcatcaa
    32401 aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc
    32461 ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta atgttatttt
    32521 cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg tcgctccaaa
    32581 gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca tagctatgca
    32641 ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac gctgaacgta
    32701 tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt ttcgaatcat
    32761 aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg atagctgatt
    32821 tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg aatgtttgcg
    32881 gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca tcatccacga
    32941 tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg
    33001 ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca ccaataacag
    33061 ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga ggttctccac
    33121 ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta tgaggcaact
    33181 tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa aaacctgttt
    33241 tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca actgatccca
    33301 tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag tcataagaag
    33361 ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt aatttatcat
    33421 aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg ttcgtaccac
    33481 taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat ttctcctgat
    33541 tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc tcgtgactcg
    33601 tacggaatcc atcaaagaaa ttcaaaaaag gtacttctcc ttcaatagaa gcaagatggg
    33661 caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata gcaaatcctg
    33721 tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca tttgtcgtca
    33781 ctgcccgact agcaacatgg aaaacacttg gcagtagttc cccagcaatc ttgtacatat
    33841 tcggtatcat caaaagcagc ccttgagaag cggtatatgt agttgtcagt gcaccagttt
    33901 tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct actactttta
    33961 ctggttgacc gaatacattt tcttttcctg ctgctgacca ttgatctact aattcagcca
    34021 tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca taagaaatat
    34081 atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat catccttgtt
    34141 catattctct cttattatct gttccattat aacgtaaaac attccatttt ttcttttatt
    34201 tataaaaata tttcacatat gtctatttga attggtcatc taatttttag acaaacagtc
    34261 taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac
    34321 atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt
    34381 tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaacc tatcttgtcc gtattgccga
    34441 caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag
    34501 cgagtgatca aattgttcga taaagaaaaa gaaagcttgc agcgaggaaa cgtgatgcac
    34561 cggtatctca caatcagcga tggtcaaaaa gcgatcaaag agatcaaaca aatattcgat
    34621 actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa
    34681 ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag
    34741 caagcacata ttcgatttgt tccgactgtc atcttgggta aagaaatctt tgatgaatct
    34801 atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa ggagcgtagg
    34861 acaaaagtaa agaacacttt tgtctcacgc tctaaacacc tataaacggc ggaagcagaa
    34921 gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcctt attccgaata
    34981 aatggtgtcc aaaagcttgc atctgccaga ttatctcatt tcttatgaca agctgagcaa
    35041 attatctcca ccaaataatt tattcatatt tttgacttgc gcatactaac taattcttaa
    35101 attttcatac aaaatttctc aaattccctt aacgagtaat atttatgtac ggtgattcac
    35161 actagtatcc caatcacttt ttaagttatc ccgatttcaa ttctacttca aaaatagttg
    35221 cttttatgta tgctgttagt tgattagatg gtcgtttgtt gagctaggtc gtttatttta
    35281 aacaagttgt ttctttagct tatacctatc tcgtctacca aacgcaagat ggcaaacaca
    35341 aatgcatacg catctgtatt tgccactttg attcgtataa agctaacaac tcctaactcc
    35401 tctcatttaa tagttgctct cttttttatt cagatacatt tttgctgatc acagcctagt
    35461 atttcaatgg ttttgaacac taaaccaaat tgttccatta caacattcac tggctgatga
    35521 aatggattaa tagatttcga ttattcaaag cgattttcta ttggtggatt gtgttcccaa
    35581 ctaacaagac ggattccaaa gtcattatat aactcttcaa gagttaaccc tttcctgttc
    35641 ccaatagatg tatctctccc acgctcgata taaaatatgg agtttcgaat gccaagacct
    35701 ggcaaatctt tccttgttac agttacactg ctactaactt ccccatataa aatccagctc
    35761 ctccaaaatg ataatatatt tttcgaccat catctaactc atgtaacaat aattgttgtg
    35821 ccctcttgtc actcgccaaa tgcaaacgcc acgtgcgata ccatgccatg ctacctgcat
    35881 ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat
    35941 ctacttcata ttctaatcta aaatcaaact cccctcgggt aatcgcaggt cttggtggct
    36001 taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa
    36061 caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt cgcaaatcac
    36121 ctctacattc tcacatacta gttcgtaata ctcctttaat atcaaaatat ctactgatcg
    36181 taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt
    36241 acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg
    36301 ttaacaatcg gttcagatat ttcccaattc actagcctta tattatattg atttaacaat
    36361 tcttcttcat ttattgtatt ttgatcaagc ggaccaaatt gttctaataa ttctttgtca
    36421 tgtaaaatat cccttctagt aatgcctctt tccctttcaa tgaaagaagc actaggaaaa
    36481 gtatgattat aaccattcaa cctatctccc atgtaataat tagcaggtcc tggaaaataa
    36541 aatatttgct tattatcact aacttctaac agcaacacta aatcttctcc gctggctaaa
    36601 cgacttgtcc atctacgata tctccctatg ccttcgttca ttccaaatcc atcaaattca
    36661 actattaacg tgtcttctat cacatgtaat tcttcattca attcatactc aagtctaaaa
    36721 ggaaattctc catatgtaat ctctggcaat ggtggtgctg gagttctcca aataaataca
    36781 ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc tgctcgaagt
    36841 aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa aataaaaagt
    36901 actgcaatca taatgatagt aaaagttgta aaaaaacgca tttttgccct ccctttataa
    36961 acatcgattc tcaaattcta aaccaaatct tggtagttca agttcaggta taacgtattc
    37021 caaccataca tctcaattac tatttatgaa cttatgatta tcactgaata aatcactcca
    37081 tccccttgtt aaaacttcta cattaattac acctggttgt tttcgactag ctaatccaat
    37141 gaacttatca accatcctta tccaccaacc acgagaatct tgaccagaag taagaagagc
    37201 agttctcaag gatgatcgag cagttccttc cagtttttta attaatcagg ccagttatag
    37261 ataaatactt gatcttatct atatcaggac ttctatatta agcaaaaatt tatgtacaaa
    37321 actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa aaacaataga
    37381 agcctttcca aattatgtta ctccagtaaa gatggaatta tagtttgacg gaagaaaagc
    37441 aagtataact tttaaaaaaa actgatacat tcgcatctcc tgcttcatgt atcagttttt
    37501 tctttggatt aaactgcttc ttcacgcagt ttttccgcaa attcatttat tttgcgaatt
    37561 cggtgattaa ttcccgattt tgatatcgca cctgagggaa tcatttcccc taattctttc
    37621 aagctgacct ctggatgttg aagccgaagt tctgcgattt cctgaagttt ttcaggaagg
    37681 gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt cgatttgttt agaagccgca
    37741 tcaatcgtct tgttcagatt tgctgtctca caattgacta gacgattgac cgaatttctc
    37801 atatcccgta cgattcgaac atcttcaaat cggagcattg agttggttgc accaatcaat
    37861 gttaagaaat cagcaatttt ttctgctcct tttaaataag aaatatagcc attgcgccgt
    37921 tcaagcgttc gagcatgtag accgtaataa tttaacatat cgcaaatgtc ttggttatgc
    37981 tgttcataaa tagaaaatat ttccaaatga taacggctcg tttccggatt attgactgaa
    38041 cctgaggcca taaaagcacc acgtaagtaa gaacgcattt tttgcgaatt tcccataatc
    38101 tcgtccgaaa cgtgtccatt gaacatcata ccatccatga tatcaagatc catcaacaca
    38161 tcttttgtgc cttgcttcaa ccggacgata tagacattgt tcttttttaa tttcattttt
    38221 ttacgaacga gtagctcact tcgtacctga taatgatctt tcaaaagaga atagatacgt
    38281 ctggcaattg cagcattttc tgtttggacg ttcaaaataa actgctggtt cacaatacct
    38341 aaagagccgt tcattcgcag caaagcagct aattccgctt tcgcatgttc tcgatgaact
    38401 tccaaaccag ttagttcttt cttcacatct gcagcaaatg acatcctatt ctcctccttt
    38461 ctccaacaat aaattgctta atattttgaa ccgaaaacta aacggaacaa ttcttctact
    38521 actttgtctc catcgtggaa gacaccacca tctttcaatt ccaaaaagtc ggtggaaatc
    38581 actcgacatc cttcatttct caatccgttg aagtcgtgtg cgacttgtac tagatactca
    38641 tcatagatct ctgggtccat ataatcttca ggcacttttt ccgtattgac caatactgta
    38701 tcgataaaag gctgattcaa atgcttgtgt aaaacacgta tatgatcggc atctgtgaaa
    38761 tgttccgttt cccctttttg ggtcatgata ttacagatat agacggtttc agcagttgtc
    38821 ttgagcattg cttcgccgat ttctggtatc actaaatttg gaaggatact ggtgaataaa
    38881 cttccgggtc ccaaaacgac catgtcggcg tccataattg ctgaaacgac ttttcgagcc
    38941 gctttggctt cacttttatc atttgtgttg cagacataca cacggtcaat ggttttacgg
    39001 tcgatcgcga ttttagattc tcctactgca gtcgttccgt ctttaaagac agcatgcaag
    39061 gtcaaaggag tttctgaaga aggataaaca tgtccatcta catgcatcat ttttgctaat
    39121 aattgaatcg cctcataagt gcttccccgc atctcggaaa ctgcggcaat gatcagatta
    39181 ccgatcgtat gattggctaa aaactgatct tcctttttga aacggtattg gaaaatatct
    39241 tcatagaatt gtggcatatc cgataacgcc actaagacat ttctcaagtc accaggagga
    39301 gccatagcga tcgattctct gattgcaccg ctgcttcccc catcatctgc aacagtcact
    39361 actgctgtga tatctgctcc ttgatttcgc aaacttttca ggataactgg cagaccagtg
    39421 ccaccgccga ccactacgat ctttggtttt cttatccggt aggttttcat tttcacgacc
    39481 ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt cacattgact ttatagtttt
    39541 tccctaaagc atttgccaaa cgttcagtca atgccacaga acggtgttga ccgcctgtac
    39601 agccgattgc aactgtcaag ctgctttttc cttctttaat gtatccaggc aatacagttt
    39661 ccagaagatc caaaaattta tggtagaatg attgcgtttc atcaaaggac atcacataat
    39721 catagactgg ctgatctttt ccggtcaatg gtcttagttc cgggatataa tgagggttgg
    39781 gcaaaaaacg cacatccatc acaatatccg aatcgatcgg caacccgtat ttaaaaccaa
    39841 atgatacaaa ttcgatccga aatccttggt ctccctttga tttgaatgac tcattgattt
    39901 tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat gatcacggaa gcttgggctt
    39961 tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc agtgatcaaa ccgtccatcg
    40021 ccattggatg tgctcgtctt gtttctttgt agcgagccac tagttcttcg tctgaagcat
    40081 ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc gatttcgatc agcatgtttt
    40141 ggatttcttc aaagaaggaa cgagaacgta aatccactcc taaagccatt tttgtgactt
    40201 tgcctgattc tttgattaat tcccagaatt ttggaatcaa tcgaggcggc atattatcga
    40261 tacaaaaata gcccatatct tcaaaacttt ggatagctac tgttttccct gcaccgctca
    40321 tacctgttat gatgactaat tgcaaattat ctgtcataat tacgaactcc tctcacattg
    40381 tgcatagtaa aattatatca ttccgggcgt atcattacca gttttctttt tcttttacaa
    40441 aaagatttgg accaaatatc ttttaggata tttgccccaa atccaatttt tttacttttc
    40501 cgatgctgta tccaatgcct tgcttctttc agctagcgct cgttcaatcg tccgatctgc
    40561 taaaatagat tgggcatcga tcactggata atggttatct tcagcaaatt catgcatcaa
    40621 tgacaattct gtgcaaccta agataacttt ttcacagttt aggcgctcta ccgcttcttc
    40681 taagatttcg taatatagct cttgattcaa ataatccgat tccttgatct catgatagat
    40741 cagataattg attttttctt gaagaacagt atcaggaatc actgtttcga atccaagatt
    40801 tttgacttcg cgttcataga ttccagcttt catacttcct tctgttccta gaatcgctac
    40861 tctccctgtc gtatgctgac ggacaagttc atttgctgct tctcttggca tatgaaggat
    40921 aggaatatcc gttgccgctt gtaattcttc aaaaaagtag tgtgcggtgt tacaagtcaa
    40981 cacaatgaag ttcggctgca ataaattttg tttctcaata tcatccagca gaaacggcat
    41041 tggattttct tctgatcgat ctaaaatata agctgtacga tcaggaactg ttgcatgatt
    41101 gaataagaca taattcaaat attcttgatc ttttgtagct ttcgttcggt ggttgatcaa
    41161 gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct aaaatactga aaaaattctc
    41221 catgaactag ccttctttca catgaaaata ctttttgaat cttggaatat agttatggaa
    41281 catatatttc atcaacaacc agcgcttgat tgaccggtct ttttcataaa acacagtagt
    41341 accgtaacga ttttctttga tcatttgctc agctagtttt ttgtcttcat tttcccgagc
    41401 atattctaaa aagatttttt tgggcacacc catccataat cttgctttat ctgattgatt
    41461 cgttccatag gtcgtttcaa caaagggttt gttaaacact cgatcttctg tgacgaaacg
    41521 agccagattc aagccgttca atgtcacaaa gaaactgcta cgaccttgtc tcaaattgat
    41581 ttcaaataat ttatattctc catctctagg atcatacttc atatcgaaat tggcaaagcc
    41641 tgtatattcg attttttcca aaaatgcctt aatggtttga tagatttttt cgttataatc
    41701 tggcatgatc acaacgtaat taccaatcga tgctggtgta ggatcttcta acagtggatg
    41761 accaagacac atcatccgaa cttgatgatc ttcatctaca tacgcattta gcacacgcat
    41821 attactatca tcacccggga taaaatcttg tacaatcatt tcacttttat aaccggcttc
    41881 gtatatccgg cctaaaatta aatcgaactc ttctctagtc tctaaaataa aagctttttt
    41941 tcttccttca aattggacag aaagatactc cacactgttt gcaggtttca aagcaactgg
    42001 gaattcaaaa ggcaattcct gttccaaatg tccatttact aacatttctt ctctgacgat
    42061 caatgtttta ggatacggaa gatcatattc ttcacaaact tcataaaagc ttactttatt
    42121 gatcagtctt tcaaataaag aataatcaat atatggacag ataaaatatt ctgacagctc
    42181 ttgtttatgt tgggagatca attctgcata tccatcacca caagcaatca aaagatattt
    42241 tttactctta tcagaatatc tttcttttgc caaacgaagc atcgtctcga taaacacagg
    42301 gtctttatca aatcctggaa taacttctac attgacaatt ttactgtagc gggtaggggc
    42361 taactgatcc gaagcatatg cttggcagat tttgccgtat gcttcattaa aagaacgtgc
    42421 cataccataa acgttcatgt cgcttcctaa taatataggg ataaattctt cattttcaat
    42481 actgttcatc attatctctc acttactcat aatctcttgt ttttcttttc caactacaca
    42541 aggaatatca taccataaag aaaaaagcaa tcaaaggaaa ctcctccgat tgctcaattc
    42601 ttttttgaac ggattgaact tataggaagt ttttgtctat accaatcagt gtagaaagaa
    42661 ctaccatgta atgattctag aacactgata ttaataaaac aagcaaaaat tcattttttt
    42721 atgttttttt aactatctca gctatctatc ctctttatac cattattttt ttcaaatagt
    42781 gacctgtata actttctttt acgttaatga tttcttctgg tgttccagtc gcaaggatcg
    42841 ttcctccacc ttctccacct tctggtccta aatcaatgat ataatctgct gttttgatta
    42901 catctagatt gtgttcaatc actaaaactg tgttaccagc atctactaat ctttgtaata
    42961 catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt cgttggttca tctagtatat
    43021 agaaattctt tccattagag attttgtgca attcactggc aagtttcatc cgttgtgcct
    43081 caccaccgga caatgtcgtt gctggttgcc ccatcgtcac ataacctaag ccaacatcaa
    43141 caatcgtttg cagtttgcga tgaatctttg gaatgtgctt gaagaattct acagcatctt
    43201 ctactgtcat ttccaaaata tcagcaatgc tttttccttt gtaatgcact tctaatgttt
    43261 cagagttata tcgtttgcca tgacatactt cacaaggaac atagacatca ggcaaaaagt
    43321 gcatttcgat cttaataatt ccatccccgc gacaagcttc acaacgaccg ccttttacgt
    43381 tgaaactaaa acgccctttc ttataacccc gcatttttgc ctcgttcgtt tgagcaaata
    43441 acccacggat atcatcaaat acacttgtat aagtcgctgg attacttctc ggcgtccggc
    43501 cgattgggct ttgatcgata tcgatgatct tttcgatact ttcgtagccg gaaattgtct
    43561 tgaatttacc tggcttagca gaattcttat ttaatttttg cgctaacgat ttctttaaga
    43621 tactattgac caatgtactc tttccagaac cagacacacc tgtgactgct acaaattctc
    43681 ctaatggaaa ttcaacacta atatttttca gattgttttc tgaagcacca gtgatcttga
    43741 ttgctttccc attaccttta cggcgttctt ttggtacagg gattgatttt ttccctgaaa
    43801 gatatttccc tgtcaaagaa ttttcatcat tagctacttc ttcaggagtg cctgcagcca
    43861 cgatttgtcc gccttgttct ccggccccag gtccgacgtc aatcagataa tctgctgccc
    43921 acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt gccaagatca cgcattttct
    43981 ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa tccgatcgat ggttcatcta
    44041 aaatatacaa aactccagaa agattcgatc caatttgtgt agccaatctg attcgctgcg
    44101 cttctccccc agataatgtt ccggaagcgc gactcaaagt tagatagtct agtcccacat
    44161 tttccaagaa tgaaagtctg tcatttactt cttttaaaat cggtttggca attactttct
    44221 cttgttcaga taacgttaag tttgagaaga aatctactgc tttttgaata gacaactcat
    44281 ttgtttgccc gatattcgtt ccattgattt gaacagatag agcttgggga ttcaatcgat
    44341 aacctttaca tgtctgacaa gttaattctg tcatataaag acgcatttgc tcacgcgtga
    44401 aatcactgtt cgtatcatga taacgacgct cgatattctt caatacccct tcgaaagtcg
    44461 tttctacatc gcgaacgcca ccaaaatcat tttcataatg gaaatggaaa agatcatctg
    44521 tcccattcaa gatgatatcc tgatgctctt ccggtaattc ttcgaatggt gtatctaaat
    44581 cgataccgaa actttcacaa gcctgcgcta acatttgagg ataatactga gagctgattg
    44641 gattccatgg aacgatagca ccttgactaa gtgtcttcgt tctatctgga atcactaaat
    44701 cttgatcgac ttctaatttg acacctaaac catcacagtc tggacaagct ccaaaaggcg
    44761 cattgaatga gaacagtctt ggttctagtt cgccaacagt aaatccgcaa taaggacagg
    44821 catagtgttc gctaaacaac atttcttctt caccgatcac gtcaaccaga gcatagccgt
    44881 ctgccaatcg cagagccgcc tctaaagaat caaatagacg agaacgaatc ccttctttca
    44941 caatgatccg gtcgatgacg atcgcgatat catgtttttt gtttttctcc agctcaggca
    45001 cttcacttaa atcatagatc tcaccatcga ctcgaacgcg gacgtatccc tctttttgga
    45061 ttcgttcgaa tattttttta tgttgtcctt ttttctttac cacgatcggt gccaacactt
    45121 ggatctttgt gcgctcaggc aaagccatta cttgatctgc catttgatct actgattgac
    45181 tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc tacacgtgca aacaacaagc
    45241 gtaaatagtc attgatttcc gtcacagtac ctactgtcga ccgaggattt ttacttgttg
    45301 ttttctgatc aatagaaatc gccggactca atccgtcgat gctatctacg tccggcttat
    45361 ccatttgtcc taaaaactga cgagcataag cagaaaggct ctctacgtat ctccgctgcc
    45421 cttcagcgta cagcgtatca aatgctaggg aactttttcc agaaccggac aaacctgtaa
    45481 cgaccacgaa tttatctcgt ggaatcgtta catctatatt tttcaagtta tgggcacgtg
    45541 caccgtgaat cgtaatttta tcgttagcca tgttgtcctc ctactttgaa gcttttaatt
    45601 ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc caatgctttc gccgcatttt
    45661 tcatttcttt ttctaatttc atcaataacg tatctttttc ttctttcgtc atttcttcat
    45721 acgagacttg taccgcttct tttgtatcat cttccgattc tttcgtaata gaaatcaaat
    45781 ctcggatttc tttaatgatc gtttttggaa cgatcccatg ttcttcattg tacttctgct
    45841 gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg catagaatct gttactttgt
    45901 ctgcgtacat gattacttta ccttcttcat tccgagcagc tcgtccaatc gtttggacta
    45961 aggaacgttc actacgtaaa aagccttctt tatccgcatc caagatagca acaagggaaa
    46021 cttcaggtac gtctaatcct tcacgaagca aattgattcc aactaacaca tcaaattcac
    46081 ctaatcgaag atctcgaatg atttctgttc gttccagcgt tttgatatca ctatgcaaat
    46141 atttgacttt gatccctaat tctttgaaat aatctgtgag gtcttctgcc atttttttcg
    46201 tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg ttcatttatt tcgcctacta
    46261 gatcatcgat ttgtcccatg attggacgaa cttcgatcac tgggtcaagc agccctgtcg
    46321 gacggatgat ttgttggata acggtatctg tttgttcatg ttcatatggt cctggcgtcg
    46381 cagagacata aatgatctga ttgacatgtt tttcaaactc ttctaaccga agtggccgat
    46441 tgtctaaagc agatggcaaa cggaaaccgt aatctactag catctgtttt ctcgctcggt
    46501 caccattata cattcctctg acttgaggca tggttacgtg cgattcgtcc gccacgatca
    46561 aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg ttcaccttct ttacgtccgt
    46621 ccatatgccg agaataattt tcaattcccg acgtgtagcc catttctaac atcatttcaa
    46681 tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag aagtttgttt tcatttcgca
    46741 agacagttaa acgctgttct aattccgctt tgatattcgc tacggcatgc tccatatgtt
    46801 catcattcgt cacaaagtga gtcgccggga agattgccac gtgttccgtt tctcctaaaa
    46861 cttcacctgt cagtgcattg acttctcgga tacgttcgac ctcatctcca aaaaactcca
    46921 cacgtaaggc acgttcatct cttgaagccg gaaaaatctc cacgacatca ccgcgcactc
    46981 gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa ttggatactg actaagtcac
    47041 gaatcaactg attgcgatct agttctgctc cctgtcgaat cgaaactact tgtttttggt
    47101 actcaaaagg cgaacctaat ccaaaaatac atgaaacgga tgcaatgacg atcacatcat
    47161 ttctttctaa taatgaacta gtagccgaat gtcgtaattt gtcgatctca tcatttacac
    47221 ttgaatcttt ttcgatatac gtatcgcttg aaggcacata agcttcaggc tgataataat
    47281 cataataaga aacaaaatat tcaacagcat tgttagggaa aaattccttg aattccccgt
    47341 acagctgacc agccaatgtt ttgttatggg caataattag tgttggttta ttcactttct
    47401 caataagatt ggaaatcgta tatgttttcc ctgttccagt cgcacctaat aagatttgtg
    47461 cttttttccc gccgactacg ccatccacta attggttaat cgcttcaggc tgatcaccag
    47521 ccggttggta tttcgagaca agctcaaagt gtcgggaggt atctctctca atcattcttc
    47581 gtcctccttt tcattcattc cttttcattt tacgaccaat aaaaagaaaa ggaaagcaaa
    47641 ggcattcctt actaaaaaat tataccacac cgaacatact ttcgccactt ttttatctta
    47701 ttatgcatgt tacgttttat aacataaaat gttagaaact cgcatttttt tcattttttc
    47761 gagcttacag tcttgtctga ttcagcactt ttcgcctata ataataaaat attggaaatt
    47821 taggaggttg attatgaaaa gaaagcattt atttcactca tttgttttct taatgacagg
    47881 acttgtgaca cttgcgtttg gacatgttgc acaagctgac aacactacaa cggcagagtc
    47941 aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa gacgtatacg taattgccag
    48001 tgactctgct tttgctccat ttgaatttca aaacgcaaag ggcgagtacg aaggcatcga
    48061 tgtaaaattg atggaaaaca tcgccaaact gcaaggattt aacatccgaa tggactaccg
    48121 tggtttctct gctgctttgc aagctttgga atcaggacaa gcagacggaa tgattgccgg
    48181 aatgactgta acagatgaac gaaagaaaag ctatgatttt tctgattctt actttgatag
    48241 cgggatccaa ttagctgtca aaaaaggaga tacttcgatc aagtcttatg aagatttgaa
    48301 aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct gaatttttaa gcgaccatga
    48361 atctgaatat ggctattcta tcaaatattt tgatgctgct gatcaattgt acgacgggtt
    48421 aaaagttggc gcaatcgatg caatgatgga tgattatccg gtaatcggtt atgcgatcaa
    48481 acaaggacaa gaattggaaa caccgatcaa aaaagaatct ggcggaaaat atgcatttgc
    48541 cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc aatgaaggat tgaaagaatt
    48601 aaaaagaaca ggcgaatacg ataagattat taaagaatat gtagcggatg gttcagaaac
    48661 ttccagtgag tcttccactg ctgatgaatc aacatttctt ggacttatcc aaaacaacta
    48721 tcaagcattg ttgaaaggac tttggagaac aattctccta actttgatct cctttgcttt
    48781 ggctatagta atcggtgtga tttttggatt attcagtgta gcaccaatac gcgctcttcg
    48841 cacaattgct agtatttatg tcgatatcat ccgaggaatt ccaatgatgg ttcttgcgtt
    48901 ctttattttc tttgggttgc ctggtatcgt tggatttacg attcctgact tccttgctgg
    48961 tgttatcacc ttgactctta acgcaagtgc ttatatcgca gagattgttc gaggcgggat
    49021 caacgccgtt cctgtcggac aaatggaagc ttccagaagt ttaggtcttt cttataatcg
    49081 gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc atgatcccat cattcgtcaa
    49141 tcagttcgtt atctcattaa aagacacaac gatcatttcc gcaatcggtg tagtcgaatt
    49201 gctgcaaact ggtaagatca ttgttgcacg tacaacacaa agtacgtatg tttacttgat
    49261 cattgcgatc atgtacttga ttttgatcac agcattgaca aaattagcaa aagttttaga
    49321 aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga gcatttagtc aaaaaatacg
    49381 gcgataacac agtattaaac gacatcaatg tgtccatcaa cgaaggagat gtcgtctgta
    49441 tcattggccc ttcaggttca ggaaaaagta catttttgcg ttgcttgaat cgtttagaag
    49501 aaccttctga cggtgaaatt attattgacg gcgcccactt gatggataaa agcacagata
    49561 tcaacttggt gagacaacat atcggaatgg tattccagca ctttaaccta ttccctcatc
    49621 tttctatttt ggaaaacatc attttagcac cgatggacct gaagaaagaa tctagagaag
    49681 aagcagagaa aaaagcaatc aaattgctgc agacagttgg tctagaagat aaaaaagata
    49741 tgtatcctga aatgctttcc ggtggacaaa agcagcgtgt ggctattgct cgtgctctag
    49801 cgatgaaccc tgacatcatg ctttttgatg agcctacatc tgcactcgac cccgaaatgg
    49861 tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca aggtatgacg atggttatcg
    49921 ttacgcacga aatgggcttc gctaaagaag tggccaatcg tgtcatgttc attgacgacg
    49981 gaaacttttt agaagacgga acgcctcaac aaatctttga aaatccgcaa aatgaacgga
    50041 caaaagattt cttgaataaa gtattaaata tttaataaaa accaaaaatg cccggtcgct
    50101 tcatgacgac cgggcatttt tagtttcttg ttttttcact taactgtacc agccgcatcg
    50161 cttgttctcc tgcacaaaca agcaatcgct ctacctgttc catcgcataa gaaagtacca
    50221 tcggttcatt gacgatatca agtacatagt cgataccacc ttcagaaact atgatgatta
    50281 tcttattcta aagctggctt ttttgttggt acaaacatga caactacaaa aaaaccaggc
    50341 gaacgtatga ttgttcgtct ggtttttaat tattaatgac ttagcgtttt tactagaaat
    50401 agaaaatttg ggcaagtcta ctttatattt ttactagaaa aactacttcc gctttttccc
    50461 gccaaaagca tctttaaact tatcgaagaa accgtcttct tgctgttctt caactttgat
    50521 gccgccagct tcagcgaatg cacgtaatgc ttctctttgt tgttcattta gatttttcgg
    50581 tgtgatcagt tttactttga catgttggtc gcctgttgca ccaccacgta acttcggagc
    50641 gccttttcca cgtaaacgga agttcgtacc tgtctgtgtt ccagcaggga tcttcaattt
    50701 cacatctcca tggacagttg gtacttggac ttcatccccc aaagctgctt gtacaaaact
    50761 taaaggcaat tcatagtaaa tttcagaacc ttcacgatca aagatatcac tttcttctac
    50821 acgaaaaacc acatacaaat caccaaatgg tccgccgttt tctccagctt ctccttgacc
    50881 agctaaacgc atttgttgtc cttcttctac accagctggt acattcactt taactgaatg
    50941 tgctttcttc tcatgtcctg agccatgaca ggttggacaa ggttctttga tttctttccc
    51001 tgtgccatga caaacatcac aagtttgacg gctcatcact cgtccaagtg gcgtttggcg
    51061 ttctacattg atcgtacctg caccatgaca tttatgacaa gtagttggtt gtgtgcctgg
    51121 ttttgcaccg tttccaccac aagtatgaca gatttcttcc cgattatact tgatttcttt
    51181 ttctacacca aaaatcgctt cttcaaaaga caagtcgacc gtatattgca aatcagcacc
    51241 ttgacgaggc gcatttggat cgactgaccg accgcctcct ccaaagaaag agtcgaagat
    51301 atcttcaaaa ccaccgaagc cgccaccaga gaagccgccg aatccaccaa atccgccacc
    51361 agctccacca gcaccgtaat ttggatctgt gcctgcatgc ccgtattggt cgtatgcagc
    51421 gcgtttttgc gggtcactta aaatctcata tgcttctgat acttctttga atttttcttc
    51481 cgcatcaggt tctttattga tgtcaggatg gtattttttc gaaagcttac gataagcttt
    51541 ctttatttca tcttctgatg ctccttttga gagccctaag acttcataat aatcacgttt
    51601 tgttgccata ggctttcctc ctattggttc tcatcatttt cacgtcgctt gttatcatat
    51661 catattttgt ttgaaacaaa aaccgatact tcaaactatt tttcaaaaga aaaagccaaa
    51721 gtagcaactt tggccttctc tcgaaattca gtgaactgat tatttatcgt cgccatcgac
    51781 ttcttcaaaa tcagcatcaa cgacatcatc tgctccgcct tgagctgctt ctggattttc
    51841 ttgagcttgt tgttgtgcag cttgttcgta tagtttaaca gtcaagtttt gaacgatttc
    51901 gttcaatgca tcgcgtttag ttttcatttc ctcgatgttg tttgcttcaa cagcggcttt
    51961 taattcgtca cgagctgttt ctgctttttt cacttcttct tcgtccactt tgccttctaa
    52021 ttctttcaat gttttgtcca cagagaatag caatgcgtct acatcattac gtaggtcaac
    52081 ttcctcttta cgagctttat ctgcttcagc gtttgcttct gcatctttca ccatacgttc
    52141 gatttcttcg tctgttagac ctgaagaaga tttgatcgtg attttttgtt ctttttgtgt
    52201 acctagatct ttcgcagata catttacaat cccgttttta tcaatatcga atgttacttc
    52261 gatttgtgga ataccacgag gagctgctgg aatatctgtc aattggaatc ttcctagagt
    52321 tttgttgtca gcagccattg ggcgttcacc ttgtaataca tggatgtcta ctgctggttg
    52381 attatctgcc gcagttgaga atacttgtga tttacttgtt ggaatcgttg tattgcggtc
    52441 aatcaattta gtgaacactc cacccattgt ttcgatacct aatgacaatg gtgtaacgtc
    52501 aagtaaaaca acatctttca catcaccagt gatcacgcca ccttggatag cagcacccat
    52561 agcaactact tcatcagggt taactgattt gtttggttct tttcctgttt ctttacgtac
    52621 tgcttctaca actgcaggga tacgagtaga tccaccgact aaaataactt cgtcgatttc
    52681 tgattgtgac agacctgcat cttttaacgc ttgacgtact ggtacttttg tacgttctac
    52741 taagtcagct gttaattcat caaattttgc acgtgtcaat gtcatttcca aatgcaatgg
    52801 tccagattct cctgctgtga taaatggcaa gctgatttgt gtgcttgata cgccagataa
    52861 atcttttttc gctttttcag cagcgtcttt caagcgttgt aaagccattt tgtcttgaga
    52921 caagtcaatg ctgttttctt ttttgaattc tgctaccatg tagtcgatga ttttgttatc
    52981 aaagtcgtcc ccacctagat ggttgtcgcc agctgttgac aatacatcaa agacaccgtc
    53041 tcctagttca aggatagata cgtcaaaagt accgccacca aggtcgaata ctaagatttt
    53101 ttcgtcgcga tctgttttat ctaaaccata ggcaagagca gctgcagttg gttcattgac
    53161 gatacgttct acttctagac cagcgatctt accagcatct tttgttgctt gacgttgtgc
    53221 atcattaaag taagctggca cagtgataac tgctttttcg actttttcac ctaaatagtc
    53281 ttctgcgaat cctttgatat attgaaggat cattgctgag atttcttgtg gtgtatatga
    53341 tttaccttct acatctactt tgtaacctgc ttcaccgata tggcgtttga ttgatgaaat
    53401 cgtatttggg tttgtcacag cttgacgttt tgcaacttcc cctacttgga tttctccatt
    53461 tttgaatgat acaacagaag gtgttgtacg gtttccttct gggttagcga tgatttttgc
    53521 ttcgtttcct tccaatactg ctactgcaga gttagtcgta cctaagtcaa taccgataat
    53581 tttactcata attaacgtct ccttctttaa ttgaaagtgt aagttttata tccgcacaat
    53641 tattgtgcaa caatgaccat agttggtctt aacacgcgat catgtaattt gtagccttct
    53701 tgtagtactt ctacaatcgt atcggctggt gtatcttcag tcgctggaac agtttgtact
    53761 gcttgatgaa gattaggatc aaaggcttct cctttagcag ggattttttc aattccttct
    53821 tcttccagtg cgttacgcaa actttccaag accatttcca cgcctttttt caaacctgcc
    53881 ccttgatcat ctgacacttc tgtggctaat gcacgttcca ggttgtcaat tgatggaagt
    53941 aattttttgg ctaaatcttg tgaacggtat ttttgtaatt gctcacgctc attttttcct
    54001 cgatttgcca tatttgcgat ttcagcacga gcccgcaagt atttatcttc catttcttct
    54061 aactcagctt ttaatttcgc tgtttcgaat tcttccgctt cagcttctga aatacccgaa
    54121 tcttctgcag cttcgacatc gatttccggt tctggctgag catctgtcat ttctttatct
    54181 agttcttctt gattttcttt cacgatacag tcttcctttc attattcagt cagctgtttg
    54241 attgctctta ctaagaaaga gagaacgatc agctgcaaat agcttcgtta ttcacattac
    54301 gagtttgaca agtcaagcga gcggtagtaa tcagcaagtt tagctgctag ttctcttcga
    54361 taaacatcga ctagaccgaa catccgagaa taaggcatac tagtaggccc taacaaggct
    54421 attgtacctc ttccatgacc atctatctcg taacttgctt gaatcatact catattctgc
    54481 aataaatcat tgcctaattc agcaccaatc ttgatatgaa tattgctatc catcggagtg
    54541 atcatttgtg taagtccgtc tgagtctttc ataaaagaaa acatcgattt gaactgatca
    54601 agatcttgac ggggctcata atccaagaga ttcatttgtc cactgacata aaccttttct
    54661 tcaaaaacat gaccaagaac ggtgtcaaat aactgagaca tcccttctgt cgtctggaaa
    54721 tatttatgaa gaatcatcgg aatctccgta cgaagtttat ggtacaccgt catcagagga
    54781 tctccgacta gacggtcgtt tacaatctgt accatttttt ctagttcctg acttcccata
    54841 tattcgggaa gagtgaacac ttggctttct acattcccca tatctgttac gacgatagca
    54901 ataatctgtc gattattcaa gggtacgata cgaaagccag tcagtcggcg gtcttttatt
    54961 tcaggtccca atgaaaaagt cgtatagctt gtcagctcag ataatatctc agcagattgc
    55021 ttaataatct cattgatttc atgaaattct ctgcctagag actgacgaat cagcagcaaa
    55081 tcatcttctg ctacgcgagc aggttttaac aaatgatcca cgtaataacg atagcctaaa
    55141 gcagatggaa tccgcccaga agaagagtga gtcttcaaca gcaatccttc atcttctaat
    55201 gctttcatat cattgcgaat cgttgctgag ctggccttga ctccctcaga cattaatgtt
    55261 ttggaaccaa cgggcacgcc actactcgta tagtgttgga tgatcagacg taaaatgtcg
    55321 ttttgtcgct ttgttaacat cttcctcacc tctcattagc actcatatat tctaagtgct
    55381 aattcactta taaatatagc aagaagccct tctcctgtca agagaaagac tccatttttt
    55441 agcactctaa acacaagagt gctaattcta aataattgat gtaaatccag ttatatcaat
    55501 acttcaatac ctcattgtca aactttctaa actttttctt ttcaagtttc gataaaaaac
    55561 aaatattctt caaactattt ttcaaataaa aagcgctcaa acacattgtt ccccaaaaaa
    55621 gtccctcttg gtgtcaaata aacacggtct tcttcattaa caagtaaccc ttcttgctct
    55681 aattccctta gcacatcacc gtacaccgat tcaattgttt tttgatagcg atcatagaaa
    55741 tgttttttag taatccctgt tttttttctt agtcctaaga acatttcttc ttctatttga
    55801 ttttttcgtg tcaatttttc ttcttccaat accgggcgtt catttgctcg taatgggcgt
    55861 aaatagtgtt ggattggtcc tttgttgcgg taacgaatat ctccaagata accgctagca
    55921 ccagctccaa acccaaaata gtggtcattg ttccagtaca tgaggttatg ttgagattca
    55981 tgtccggaga gcccaaagtt acttatttca tattggtgac gtccatgctt ttccattgct
    56041 tcaatcgtct cttcaaacat tctggtttcc gtttcctgat caggcagttc gagtcttcct
    56101 tgtcgtaccc agttcatgaa cattgttttg ttttcgagga tcaatgagta caaagaatag
    56161 tgaggcagat ccaaagccaa agctttttcc aaagtatccc gatttccttc tagtgtttgt
    56221 cccggtaatg cataaatcaa atcgatgctt acattggtaa aattttctgc ttctaaaaat
    56281 tgcattgttt gatacacatc ttctgcagta tgtttacgtc caattttttt caataagcga
    56341 ttgtcaaatg tctgcactcc catggataaa cgattgacgc catagttttt catcacttga
    56401 agtttttcac gggtcaaatc accaggattc gcttctacag taaactcatt accattttga
    56461 aaaggcagga tctctctagc acctgataat agtcgatcaa gttgtttagc agataaagaa
    56521 gttggtgttc cgcctcctac ataaagtgtt tccaaattcg ctgtaggatg actttccagc
    56581 attaatctca tttcattcag cagcatttcc acatactcat ctactggctg cccttctaaa
    56641 aaaactttat taaagtcaca gtaatagcag atatgctcgc aaaatgggat atgaatgtac
    56701 gcagaaacct tgttttctgc gggatttatt gtttctatca ttgaaattct ttctccttac
    56761 gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc gttcaagcac caactcattg
    56821 aaataagccg catatttaga atcaaggaaa cctagctcta aaaatgcttg cttattgtca
    56881 tcggagctac acggtgcttt tacagcctct cgataaacgg taatcgcaaa gctgaccttc
    56941 ggcattaagt caaaatctgc aaaaatatga aaagctattt tcacagattc ctccttaagt
    57001 gctcagacca caacgctttg cttacaacct ctcatatacg catactaaac gtatttggcg
    57061 aacaaatcca ctaaaaaatc caagaatcga cttcttggat gattttttta cactcatgat
    57121 tcttcctaaa caattttgtc cttattttat tcataaaaaa atctagtcaa atctctcact
    57181 tcaagagttt taactagatt ccaattatta tttttttgta aaaataaaca gtctcttaat
    57241 catagacatt tcttcggtgc cctacttgaa tagcgataac aacgaattga ttatcttcta
    57301 tctctaccaa tacacgtatt tgccaatcag agctgcttct gaatttcggg cgtgaatttt
    57361 aaacgacagg tcattccagc ccaccaaatt cattaaggat tcttccatga atactgttgt
    57421 tttcccactt cttatccaat gtttatgggc aatttcagcc gtttgacgat tgtattcatc
    57481 ttctagttgt tccatcgaat agttttttag aagttcagat aaagtaatcc cataaaattc
    57541 agccatataa gaaagccatt cttttttggc gtcacttaca cgaaccgtaa tcatagccct
    57601 ataattgcct ctcttaaaga atctcatgca tttcctgatg tgattttaga aaaatttact
    57661 tgctaaaaaa ctgtgaaaat gtaggagcta gcatccttgt agtaaatgat cgagctcttt
    57721 attatacatt aaaaaagttc agaaattttg atgaagtagc caactacttt ttcatgagta
    57781 aagcaacact agaaaaagaa tcctccagta cttagaattt actggaggat gtacttttga
    57841 agaagcctat aggttattta aaaaatatta ctacaggaag gaacgagaat tacttcgtta
    57901 cttataatag aacttagtct tatatagaag gcatagctca cattagctta tttgtcctat
    57961 aaaaataaat tacggtattt tagaaaagct attttctctt ttttcaacga tcttcagcaa
    58021 tttttcttta tcgtcaggta actcaagttc atgaattcta gcatgacaat tgggacataa
    58081 cgcgatacag ttttcaatca catcttttcc accttgcgac aaatattcta tatgatgaca
    58141 atgtaaataa ggtacaccgt cttttataaa tggccctttt tcttggcaaa gttgacaaat
    58201 accttttgca agtcgtttaa catattcaga tacgaaggga ttacgtttat atatttttgt
    58261 ttttgagaag caaaactcct cttcgttaac atattttgca gccatttttt tattataatt
    58321 actaactttt cttgcttctt cctctaattt agcattagat aaattacgaa tatgtctagt
    58381 ttgattttct tcctggttat atatctttat cttcaatcaa ttgctcttga tttattaaag
    58441 ctaatggaaa tttatatact tttctgctgt tgcccgaact atctttttca ttaacagtat
    58501 atatttgtcc tgctaaatat acttctccca gatatgtgtg ttcttttggt ttaaatgttt
    58561 caaataaatg aatctttaca aaattacttt ttgaattggc taaaacttta ttttgattct
    58621 tatccaatac ttgatcccct tttagcccca taccggtata ttgtaaaata ccatttaacc
    58681 acttatcttt ataaacatta cttcctgttt gatcactaaa cagtacaaga gtattagttt
    58741 gatgagatct tctcattcca ccctgtttgg aacatttaaa cttctctgaa atttctttgt
    58801 tagtatatac tctaccaact tctaactcac ctaatatgtt tttagaccaa ttttctcgcc
    58861 atgtaataat cttatcaaga ttaaacattt ctccctcttt tggcatgcca tttctaatta
    58921 aattagctaa tacaccagta gaaatatcta atttttttag taattctttc ttagtaatca
    58981 atgttggatt cataacaatg cagctcctaa tgatatcaat ataattgcat tagataactt
    59041 gaaaaatcga aaaaaactca ttttgtgtca agttatctgt aatatataaa aatatcatta
    59101 ttatacctca tctatgtgaa tttttactat tcttacctta gcatattctt tataggtata
    59161 tcagagctaa tcaaactaaa aaatacccga agtcttctta tgtaaaaaca ccggttatta
    59221 cttaaatatg acttaggtaa aacaatgagt gatccaatta taaaatttta tggcgaataa
    59281 tttagtaagc gctacatatt gaatgattta gaaattttga aaaagtagct agttcctttt
    59341 ataccagaaa agtaaaccta ataaaaaaca caactaattt ttagctgtgt tttttaagtt
    59401 acaataatta tctatttaat agtcttaatt cattaaaata aaaaactcaa ctttacaagg
    59461 atatggttta aaaaatttat ttatccaacc caactagttt tcgctgctta cgatttgctt
    59521 catctgctac gtaattcatt actgttttaa attctttcgc tgaggcgttt tctgataaat
    59581 cagcgatact tttattatac ttcttcacga tttgttgtac ttgttctttc tcagtcatgt
    59641 aattcctcct ccaactcttt tgaagtatat tgtattggta gtactaattg aatattatct
    59701 gctgcataat gcacttcaga aatgtcgttt tgataatcct taatcattat gtgaaggact
    59761 acttcaggat gtctattttt gactttttca atattctctc tggacttaaa aaacgcattg
    59821 ataaacattt cttttggcac atgacgtcct tcagctagtt cacgttttcg agtgaaatcc
    59881 caggcaactt tgggatcttg gtaaacatag aaaatcgtaa cgcgaaagtt atttttaagg
    59941 gctcgaataa tatttttctc tgcactaagg atagcaaaag tatcctctag tatgaaagaa
    60001 tagccatgtt ccagtacata ttttaatgat tgttcaacta accaagaaga agctttctga
    60061 aaaacacttg agttctttcc attgtattct ggaaattgtg ctctaaaata gtccgcatca
    60121 atcacaacat ggttgtcata attttaagct aaacctagtg caacctctgt tttacctgct
    60181 cctgggcttc cagccatgaa taccgcatct ttttcatcat cagggatttt tcccttgatg
    60241 attgtttcaa taaatttatc tttattgacc ctagcgtaat ctaaatactt gtctttaggt
    60301 tccaagattg caccttcttt ccttcttcta tattcatact aattatacga tatttatata
    60361 aaaaatataa cggtcatttt aaccatatta acatttagtc acagtaatag cagatatgct
    60421 cgcaaaatga gatatgaatg tacgcagaat ccttgttttc tacggcattc gctttttttt
    60481 cattggacta gttatatatt tctagggttg ttttcatgtc tgagtgcccg atatgattta
    60541 tttttcgttt ctataattac tttttgcctt ccagtttctt ttaagattgt actcaagcaa
    60601 gtgggaattg gttcttttgt catccatgta tgcaaaatgt tataatgctg gtaaacaaaa
    60661 atgattacct gtcattttta tttagttcgt atttaattaa gttgactgtc tgtatactct
    60721 gtctaaaact taagaattgt tgttttcgct gtcgctatcc aaatttaatg aatgtctgta
    60781 tgttaaaact gttgtccttg tcatagatag taaaggagag cttgaaaatg aaaactgcga
    60841 ttacgtttct attagaggaa tatgccgatt gggaaggtgc ttatattact agcacactaa
    60901 accaaaaaga gggatggcaa aacaaaactg cttctatcca atcagaggtc acttccattg
    60961 gaggattaaa aacaaaaatc gattacttaa tcgaacaaat acctgaaaaa gttgaccttt
    61021 ttattatgat cggtggcaat tcatgggaca taaaaaatca tcatttatat caaataatcg
    61081 ataaattgtt aaaaacagac gtaccagtag ctgctatttg tggggcagtt gattatttag
    61141 ctcagagcgg attattgcaa ggttacaaac acaccggaaa tgcgcaattt ccttggttag
    61201 aatcggaaaa ttatcagaat aaagaagatt ttatagaaca acaagctgtg aaagatcgta
    61261 atttgattac tgcgaatgga acagctccta tagaattcac agacttgatt cttcaagcag
    61321 tccattttga aacagaagaa ataagagaaa aaaagattcg tctatacaaa cttggattct
    61381 atgattacta taaaaagtac ggagatcctt tctcctaatc attctcttca attaaatact
    61441 ccttatcaat agtatgacaa gtaattttac tgatggttat ttgtcacact ttttctgaca
    61501 tttatcaaag aagatacact ctatttatat tcat
//
LOCUS       DACIWG010000011        59637 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.011, whole genome shotgun sequence.
ACCESSION   DACIWG010000011 DACIWG010000000
VERSION     DACIWG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.011"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(451..1761)
                     /locus_tag="IYF54_000665"
     CDS             complement(451..1761)
                     /locus_tag="IYF54_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ9831018.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="IYF54_000666"
     CDS             complement(1773..3191)
                     /locus_tag="IYF54_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9831019.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="IYF54_000667"
     CDS             complement(3431..4999)
                     /locus_tag="IYF54_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ9831020.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="IYF54_000668"
     CDS             complement(5003..5413)
                     /locus_tag="IYF54_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ9831021.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="IYF54_000669"
     CDS             complement(5410..6333)
                     /locus_tag="IYF54_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ9831022.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="IYF54_000670"
     CDS             complement(6330..7208)
                     /locus_tag="IYF54_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ9831023.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="IYF54_000671"
     CDS             complement(7195..8532)
                     /locus_tag="IYF54_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ9831024.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="IYF54_000672"
     CDS             complement(8535..9638)
                     /locus_tag="IYF54_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ9831025.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="IYF54_000673"
     CDS             complement(9712..11220)
                     /locus_tag="IYF54_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9831026.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="IYF54_000674"
     CDS             complement(11186..11878)
                     /locus_tag="IYF54_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9831027.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="IYF54_000675"
     CDS             complement(11900..12874)
                     /locus_tag="IYF54_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831028.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="IYF54_000676"
     CDS             13174..14046
                     /locus_tag="IYF54_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9831029.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="IYF54_000677"
     CDS             complement(14504..16702)
                     /locus_tag="IYF54_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831030.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="IYF54_000678"
     CDS             16934..17926
                     /locus_tag="IYF54_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9831031.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="IYF54_000679"
     CDS             17986..19407
                     /locus_tag="IYF54_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ9831032.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="IYF54_000680"
     CDS             19400..20899
                     /locus_tag="IYF54_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ9831033.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="IYF54_000681"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="IYF54_000681"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ9831034.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="IYF54_000682"
     CDS             22491..23174
                     /locus_tag="IYF54_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ9831035.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="IYF54_000683"
     CDS             23176..24012
                     /locus_tag="IYF54_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ9831036.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="IYF54_000684"
     CDS             24023..25777
                     /locus_tag="IYF54_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831037.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRIYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="IYF54_000685"
     CDS             complement(26053..26394)
                     /locus_tag="IYF54_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ9831038.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="IYF54_000686"
     CDS             complement(26462..28633)
                     /locus_tag="IYF54_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ9831039.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="IYF54_000687"
     CDS             28844..29701
                     /locus_tag="IYF54_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9831040.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="IYF54_000688"
     CDS             complement(29772..30629)
                     /locus_tag="IYF54_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9831041.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="IYF54_000689"
     CDS             complement(30614..33316)
                     /locus_tag="IYF54_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ9831042.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="IYF54_000690"
     CDS             complement(33329..34618)
                     /locus_tag="IYF54_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ9831043.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="IYF54_000691"
     CDS             34768..35814
                     /locus_tag="IYF54_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9831044.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="IYF54_000692"
     CDS             35816..37969
                     /locus_tag="IYF54_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ9831045.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="IYF54_000693"
     CDS             complement(38442..39893)
                     /locus_tag="IYF54_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831046.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="IYF54_000694"
     CDS             complement(39890..41614)
                     /locus_tag="IYF54_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9831047.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="IYF54_000695"
     CDS             complement(41607..42221)
                     /locus_tag="IYF54_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9831048.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="IYF54_000696"
     CDS             complement(42566..44023)
                     /locus_tag="IYF54_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9831049.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="IYF54_000697"
     CDS             complement(44064..44984)
                     /locus_tag="IYF54_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9831050.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="IYF54_000698"
     CDS             complement(44996..45943)
                     /locus_tag="IYF54_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831051.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="IYF54_000699"
     CDS             complement(46422..47141)
                     /locus_tag="IYF54_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ9831052.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="IYF54_000700"
     CDS             complement(47190..48836)
                     /locus_tag="IYF54_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9831053.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="IYF54_000701"
     CDS             49015..49605
                     /locus_tag="IYF54_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9831054.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="IYF54_000702"
     CDS             49697..51202
                     /locus_tag="IYF54_000702"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ9831055.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IYF54_000703"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="IYF54_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ9831056.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="IYF54_000704"
     CDS             complement(52638..53156)
                     /locus_tag="IYF54_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831057.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="IYF54_000705"
     CDS             complement(53172..53498)
                     /locus_tag="IYF54_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ9831058.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="IYF54_000706"
     CDS             complement(53511..55490)
                     /locus_tag="IYF54_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9831059.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="IYF54_000707"
     CDS             complement(55511..55825)
                     /locus_tag="IYF54_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ9831060.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="IYF54_000708"
     CDS             55993..56286
                     /locus_tag="IYF54_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ9831061.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="IYF54_000709"
     CDS             complement(56364..57476)
                     /locus_tag="IYF54_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9831062.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57863..58042
                     /locus_tag="IYF54_000710"
     CDS             57863..58042
                     /locus_tag="IYF54_000710"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831063.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="IYF54_000711"
     CDS             58236..58406
                     /locus_tag="IYF54_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9831064.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..59570)
                     /locus_tag="IYF54_000712"
     CDS             complement(58830..59570)
                     /locus_tag="IYF54_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9831065.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17824 a        12190 c        10426 g        19197 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aatttattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc atacccc
//
LOCUS       DACIWG010000012        59618 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.012, whole genome shotgun sequence.
ACCESSION   DACIWG010000012 DACIWG010000000
VERSION     DACIWG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.012"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            189..419
                     /locus_tag="IYF54_000713"
     CDS             189..419
                     /locus_tag="IYF54_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ9831066.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="IYF54_000714"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="IYF54_000714"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ9831067.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="IYF54_000715"
     CDS             1830..2006
                     /locus_tag="IYF54_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ9831068.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="IYF54_000716"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="IYF54_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ9831069.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="IYF54_000717"
     CDS             2622..3089
                     /locus_tag="IYF54_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831070.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="IYF54_000718"
     CDS             3174..4058
                     /locus_tag="IYF54_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9831071.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4175..4408
                     /locus_tag="IYF54_000719"
     CDS             4175..4408
                     /locus_tag="IYF54_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ9831072.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="IYF54_000720"
     tRNA            4496..4576
                     /locus_tag="IYF54_000720"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="IYF54_000721"
     tRNA            4583..4654
                     /locus_tag="IYF54_000721"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            5169..5876
                     /locus_tag="IYF54_000722"
     CDS             5169..5876
                     /locus_tag="IYF54_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9831073.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="IYF54_000723"
     CDS             complement(5935..6579)
                     /locus_tag="IYF54_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ9831074.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="IYF54_000724"
     CDS             6705..6992
                     /locus_tag="IYF54_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831075.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="IYF54_000725"
     CDS             7100..7420
                     /locus_tag="IYF54_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831076.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="IYF54_000726"
     CDS             7501..8814
                     /locus_tag="IYF54_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9831077.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="IYF54_000727"
     CDS             8811..9530
                     /locus_tag="IYF54_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ9831078.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="IYF54_000728"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="IYF54_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ9831079.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="IYF54_000729"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="IYF54_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ9831080.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="IYF54_000730"
     CDS             join(13091..13153,13155..14192)
                     /gene="prfB"
                     /locus_tag="IYF54_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ9831081.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="IYF54_000731"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="IYF54_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ9831082.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="IYF54_000732"
     CDS             14967..15860
                     /locus_tag="IYF54_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831083.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="IYF54_000733"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="IYF54_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ9831084.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="IYF54_000734"
     CDS             17797..18654
                     /locus_tag="IYF54_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ9831085.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="IYF54_000735"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="IYF54_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ9831086.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="IYF54_000736"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="IYF54_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ9831087.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="IYF54_000737"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="IYF54_000737"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831088.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="IYF54_000738"
     CDS             21410..22168
                     /locus_tag="IYF54_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831089.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="IYF54_000739"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="IYF54_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ9831090.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23095..24126
                     /locus_tag="IYF54_000740"
     CDS             23095..24126
                     /locus_tag="IYF54_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ9831091.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24123..24890
                     /locus_tag="IYF54_000741"
     CDS             24123..24890
                     /locus_tag="IYF54_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ9831092.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="IYF54_000742"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="IYF54_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ9831093.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26858..27175
                     /locus_tag="IYF54_000743"
     CDS             26858..27175
                     /locus_tag="IYF54_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ9831094.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27175..27525
                     /locus_tag="IYF54_000744"
     CDS             27175..27525
                     /locus_tag="IYF54_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ9831095.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /locus_tag="IYF54_000745"
     CDS             27692..28630
                     /locus_tag="IYF54_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ9831096.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /locus_tag="IYF54_000746"
     CDS             28645..29475
                     /locus_tag="IYF54_000746"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ9831097.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="IYF54_000747"
     CDS             29519..30544
                     /locus_tag="IYF54_000747"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ9831098.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="IYF54_000748"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="IYF54_000748"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ9831099.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="IYF54_000749"
     CDS             31622..32062
                     /locus_tag="IYF54_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ9831100.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="IYF54_000750"
     CDS             32065..32667
                     /locus_tag="IYF54_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ9831101.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="IYF54_000751"
     CDS             complement(32726..33832)
                     /locus_tag="IYF54_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ9831102.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="IYF54_000752"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="IYF54_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ9831103.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="IYF54_000753"
     CDS             complement(35380..37725)
                     /locus_tag="IYF54_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ9831104.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="IYF54_000754"
     CDS             38004..38906
                     /locus_tag="IYF54_000754"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ9831105.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="IYF54_000755"
     CDS             39119..40894
                     /locus_tag="IYF54_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ9831106.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="IYF54_000756"
     CDS             complement(40960..42546)
                     /locus_tag="IYF54_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ9831107.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42797..43021
                     /locus_tag="IYF54_000757"
     CDS             42797..43021
                     /locus_tag="IYF54_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ9831108.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="IYF54_000758"
     CDS             43163..44914
                     /locus_tag="IYF54_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9831109.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="IYF54_000759"
     CDS             44914..46698
                     /locus_tag="IYF54_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831110.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /locus_tag="IYF54_000760"
                     /pseudo
     CDS             47024..47875
                     /locus_tag="IYF54_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            47929..49680
                     /locus_tag="IYF54_000761"
     CDS             47929..49680
                     /locus_tag="IYF54_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9831111.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49684..49854
                     /locus_tag="IYF54_000762"
     CDS             49684..49854
                     /locus_tag="IYF54_000762"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831112.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49894..50796)
                     /locus_tag="IYF54_000763"
     CDS             complement(49894..50796)
                     /locus_tag="IYF54_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9831113.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51027..52430
                     /locus_tag="IYF54_000764"
     CDS             51027..52430
                     /locus_tag="IYF54_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9831114.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52430..53863
                     /gene="ascB"
                     /locus_tag="IYF54_000765"
     CDS             52430..53863
                     /gene="ascB"
                     /locus_tag="IYF54_000765"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ9831115.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54008..54934
                     /gene="trxB"
                     /locus_tag="IYF54_000766"
     CDS             54008..54934
                     /gene="trxB"
                     /locus_tag="IYF54_000766"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ9831116.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55148..56875
                     /locus_tag="IYF54_000767"
     CDS             55148..56875
                     /locus_tag="IYF54_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ9831117.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56947..57240
                     /locus_tag="IYF54_000768"
     CDS             56947..57240
                     /locus_tag="IYF54_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ9831118.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57332..58192)
                     /locus_tag="IYF54_000769"
     CDS             complement(57332..58192)
                     /locus_tag="IYF54_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ9831119.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58284..58712
                     /locus_tag="IYF54_000770"
     CDS             58284..58712
                     /locus_tag="IYF54_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9831120.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58874..59050
                     /locus_tag="IYF54_000771"
     CDS             58874..59050
                     /locus_tag="IYF54_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ9831121.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59077..59523
                     /locus_tag="IYF54_000772"
     CDS             59077..59523
                     /locus_tag="IYF54_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ9831122.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19420 a        10338 c        12778 g        17082 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
    13261 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
    13321 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
    13381 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
    13441 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
    13561 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
    13621 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    15121 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggtttca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaaaat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtt cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgatatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc tgtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aaaggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtag gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcgc aaacattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac tggtagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgattgtga agaacaagta
    39481 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttattagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcaatggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac tattttttaa gtgtctacac
    40801 tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccgt
    40981 ttcctgcaaa agctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggaa tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca aagcggcatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca aaataccatt
    41581 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac
    41641 agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc
    41701 cattgacaac tggatattag aatcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgttttgg tgatcgatct tccgagtttg tgaaactgct gaatgaagat ataagttatg
    41821 cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt cgccgccgat
    41881 gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacctaatg tcttacttgt gcgtgctcgg attgcctgtg ccaaatccat
    42241 ttggctcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga
    42361 ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttat tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaagattc ggtacgccta attttttttc atggcgcgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga
    45481 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac
    46081 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga
    46801 tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac
    46861 aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg
    47101 aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acgggctttc acaacagttc taagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca
    47281 agtccagttg aatgattctg tgtatattgc attgacggat catttgtata cggctatcga
    47341 acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt
    47401 tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt tactataaca atacaggata ttctaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt
    47641 cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga
    47701 agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaaaaaa
    47881 ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct
    47941 tccactaata aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc
    48001 gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt
    48061 cgtttcaaat taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt
    48121 attgtaacgg taatgcgagc gggagggcag tatcaagttg tcattgggaa tcatgtgcca
    48181 gtcgtgtttg aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca
    48241 gaaaaaatga gaccgtttga tatgcttatt gatggaatat cgggctgttt ccagccattc
    48301 cttgctatcc tagctgctgg cgggatgatt cgtggactga cagcattcct tgtcttctta
    48361 ggcgcgtttg accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta
    48421 ttccaattta tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa
    48481 ttcgtaggga tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg
    48541 agtggtgcag cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct
    48601 atttatcaaa cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt
    48661 cccattatct ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta
    48721 cctgagatga tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta
    48781 ggttttttac tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt
    48841 gaaacacttt tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa
    48901 attctggtga tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct
    48961 gtgaatggtt ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca
    49021 gcacttaccg cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca
    49081 gccattgttt ctggtattgt ggggattaca gagccagcta tttatgggtt tacattacca
    49141 cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg
    49201 ttaatgaatt taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat
    49261 attagaccag atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg
    49321 atggtggtat cctttacgct gacttttttc ttttggaagg atgaagcagg tgaaacagat
    49381 gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct
    49441 ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg
    49501 atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc
    49561 ccgacgaaac aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc
    49621 ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa
    49681 agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga
    49741 tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa
    49801 aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt
    49861 aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt
    49921 gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga
    49981 aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc
    50041 tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc
    50101 gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact
    50161 ggtcaaagct aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac
    50221 attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc
    50281 ttcatagaat gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat
    50341 tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt
    50401 ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag
    50461 tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc
    50521 gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc
    50581 tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc
    50641 tacacggatg agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat
    50701 ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga
    50761 aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt
    50821 tacgtaaatt ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa
    50881 aatagacatt ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa
    50941 atgttactgg aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa
    51001 gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg
    51061 gaggcagcag ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac
    51121 cgatacgaga aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta
    51181 gtcagatgtc tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc
    51241 tggatcggga agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc
    51301 aattagatgc acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag
    51361 aaacaaatgg tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa
    51421 tcgttgagga catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa
    51481 gtgggatggt gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat
    51541 gcaatgtgcc agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag
    51601 agctgatcta caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata
    51661 aaggaaatga ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac
    51721 cgaaaaatat ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc
    51781 cttgtggcta ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag
    51841 aatatcaaac gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg
    51901 aattatacaa agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg
    51961 cttattattc agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac
    52021 agatcgtggt atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag
    52081 tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc
    52141 ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag
    52201 cagctgtgac tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac
    52261 gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt
    52321 taccgcaatt caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg
    52381 aactggcaag agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag
    52441 aaaagatttt ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga
    52501 aggtggacgc ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt
    52561 tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat
    52621 tgcaatcgat atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt
    52681 caagacttat cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga
    52741 gccgaatgaa gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg
    52801 aattgagcca cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata
    52861 tggtgcgtgg cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt
    52921 caatcgctat aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct
    52981 tcatgcacca ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat
    53041 caaatatcaa gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca
    53101 tgaagtagat ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata
    53161 cacttgcaat cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt
    53221 cattgatgtc cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa
    53281 tggcattcag atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga
    53341 ttttatctct ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga
    53401 acagactgca ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg
    53461 ggggtggcaa attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta
    53521 tcaaaaacca ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa
    53581 tggttatgtg gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa
    53641 ggatgcagta gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga
    53701 tttagtatca gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg
    53761 tgataaccaa ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa
    53821 aaaagtaatt gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag
    53881 tctattgttt ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga
    53941 acaatagact ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa
    54001 tctacatatg tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt
    54061 atatgcttca cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca
    54121 aatgaacaat acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga
    54181 tctagcttat aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat
    54241 cgttcaagat atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta
    54301 cgaaggaaaa acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg
    54361 cgaggaagaa tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt
    54421 ccgtaacaaa cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta
    54481 tttgacacaa tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca
    54541 aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt
    54601 cgttgacgaa atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa
    54661 aaccggcaat gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc
    54721 attaactgca ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga
    54781 caacgaaatg aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga
    54841 tttgcgacaa atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa
    54901 atacttggag tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt
    54961 caattgttct tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca
    55021 cgtccaacac cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    55081 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    55141 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    55201 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    55261 ttatgcaccg ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa
    55321 tcgaatgaac atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac
    55381 acaaggtgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc
    55441 accagaattt gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt
    55501 attcgaaagt ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt
    55561 tgcgggaatc atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta
    55621 cggagaagat ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    55681 tgaagtcagc aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg
    55741 gttgatcacg atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt
    55801 gacgattgat cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc
    55861 acttcatgga acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    55921 attcgtacta gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc
    55981 aaatccagaa gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc
    56041 tgacttgctg attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc
    56101 agatggttct tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat
    56161 tttggaagca catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat
    56221 cgtttccagc gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt
    56281 gttgacaggg tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca
    56341 tacttttatg tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga
    56401 taaagacgcg atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca
    56461 aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga
    56521 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    56581 ggaaaaattc cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga
    56641 agactttaaa gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc
    56701 gccatccgac gtattgaagt atacattgga agaaaatggc tggattgcta tccgcccttc
    56761 aggaactgag ccgaaaatca agttttatat tggtgtaaaa ggaagttcta cagaagacgc
    56821 agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac
    56881 ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg
    56941 aatggattgc cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg
    57001 ttgagtgatc ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg
    57061 acagcaatca cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta
    57121 ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt
    57181 gatgattctc atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa
    57241 cgataggtgc aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact
    57301 ccagtgtttt ttaagaaaaa gacaaaaatg atcaatcgct tttttctgtt gtttcagttc
    57361 cataataact atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa
    57421 tagataaaga tgctacattg atcactacac atcctagttt ttcataaaga tcattcgcaa
    57481 aatcgagttc agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt
    57541 aagagcgcat ccgttcttta cggatattat ttaaaatatc tgggttattt gttagtccca
    57601 cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt gggatcaaag
    57661 gtaaattggc aacttttaaa tttttgttag ctaaaaacaa gctcaaaggc gttttggaag
    57721 ttcgggatac gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat
    57781 catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata
    57841 aatggagggc accaggttgc cgaattggct tgataccagt taaacgttca atttctgata
    57901 tcggtggcgt catcacatca aaactaaata attgatgttc ttcacaaaaa gaacgtgtta
    57961 tctcgatcag ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat
    58021 cttgcaatgc ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga
    58081 actcaacggt aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag
    58141 cagagtcgga gatgacaaaa aaagtaacga tttctgtaga atcggaagtc atggataaaa
    58201 cctcacttgc atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc
    58261 attttgattt ggagggataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa
    58321 cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag
    58381 tgaagcgtaa ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca
    58441 aaggggtaag ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac
    58501 tggaagaaac cgatttaaat ggagaaatga agtttcgttt tcattgctgt caggggaaaa
    58561 tcggccatca ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt
    58621 gtccgatgaa ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg
    58681 agattttcgg aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat
    58741 tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg
    58801 acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag
    58861 ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct
    58921 tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga
    58981 attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa
    59041 aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac
    59101 gatgacatga aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg
    59161 atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa
    59221 gaactaacgg tgttgtctcg tgaaatgaaa cagcgacgtg attctttgca tgagtttgaa
    59281 gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa
    59341 tatatgccgg aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact
    59401 caaactggtg cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa
    59461 gtcaaaggaa aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct
    59521 taattttatc atttaaactg cggacagtga ttatccgcag ttttttgtgt tcgtaaaaaa
    59581 agaactaaaa aacagctaga agaaagaatc ttattctt
//
LOCUS       DACIWG010000013        57516 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.013, whole genome shotgun sequence.
ACCESSION   DACIWG010000013 DACIWG010000000
VERSION     DACIWG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.013"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(61..2424)
                     /locus_tag="IYF54_000773"
     CDS             complement(61..2424)
                     /locus_tag="IYF54_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ9831123.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IYF54_000774"
     CDS             2872..3093
                     /locus_tag="IYF54_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831124.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IYF54_000775"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IYF54_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ9831125.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IYF54_000776"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IYF54_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ9831126.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IYF54_000777"
     CDS             complement(5894..6205)
                     /locus_tag="IYF54_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ9831127.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IYF54_000778"
     CDS             complement(6202..6495)
                     /locus_tag="IYF54_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ9831128.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IYF54_000779"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IYF54_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ9831129.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IYF54_000780"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IYF54_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ9831130.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IYF54_000781"
     CDS             complement(8333..12685)
                     /locus_tag="IYF54_000781"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ9831131.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IYF54_000782"
     CDS             complement(12897..14606)
                     /locus_tag="IYF54_000782"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ9831132.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IYF54_000783"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IYF54_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ9831133.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IYF54_000784"
     CDS             complement(16103..16903)
                     /locus_tag="IYF54_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ9831134.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IYF54_000785"
     CDS             complement(16900..17712)
                     /locus_tag="IYF54_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ9831135.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IYF54_000786"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IYF54_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ9831136.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IYF54_000787"
     CDS             complement(18467..19189)
                     /locus_tag="IYF54_000787"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ9831137.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IYF54_000788"
     CDS             complement(19325..20206)
                     /locus_tag="IYF54_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ9831138.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IYF54_000789"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IYF54_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ9831139.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IYF54_000790"
     CDS             21446..21925
                     /locus_tag="IYF54_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ9831140.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IYF54_000791"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IYF54_000791"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ9831141.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IYF54_000792"
     CDS             23226..24011
                     /locus_tag="IYF54_000792"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ9831142.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IYF54_000793"
     CDS             complement(24357..26048)
                     /locus_tag="IYF54_000793"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ9831143.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IYF54_000794"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IYF54_000794"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ9831144.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IYF54_000795"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IYF54_000795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ9831145.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IYF54_000796"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IYF54_000796"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ9831146.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IYF54_000797"
     CDS             30332..31033
                     /locus_tag="IYF54_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ9831147.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IYF54_000798"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IYF54_000798"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ9831148.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IYF54_000799"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IYF54_000799"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ9831149.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IYF54_000800"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IYF54_000800"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ9831150.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IYF54_000801"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IYF54_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ9831151.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IYF54_000802"
     CDS             complement(34528..35508)
                     /locus_tag="IYF54_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9831152.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IYF54_000803"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IYF54_000803"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ9831153.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IYF54_000804"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IYF54_000804"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ9831154.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IYF54_000805"
     CDS             38266..38433
                     /locus_tag="IYF54_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ9831155.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IYF54_000806"
     CDS             complement(38495..40306)
                     /locus_tag="IYF54_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ9831156.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IYF54_000807"
     CDS             complement(40303..40668)
                     /locus_tag="IYF54_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ9831157.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IYF54_000808"
     CDS             complement(40831..41226)
                     /locus_tag="IYF54_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9831158.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IYF54_000809"
     CDS             complement(41244..42206)
                     /locus_tag="IYF54_000809"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ9831159.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IYF54_000810"
     CDS             complement(42206..42418)
                     /locus_tag="IYF54_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ9831160.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IYF54_000811"
     CDS             complement(42439..43137)
                     /locus_tag="IYF54_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ9831161.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IYF54_000812"
     CDS             complement(43157..43699)
                     /locus_tag="IYF54_000812"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ9831162.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IYF54_000813"
     CDS             complement(43831..44835)
                     /locus_tag="IYF54_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ9831163.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IYF54_000814"
     CDS             complement(44832..45821)
                     /locus_tag="IYF54_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ9831164.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IYF54_000815"
     CDS             complement(45818..46624)
                     /locus_tag="IYF54_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831165.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IYF54_000816"
     CDS             46790..47746
                     /locus_tag="IYF54_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9831166.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IYF54_000817"
     CDS             complement(47823..48341)
                     /locus_tag="IYF54_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9831167.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IYF54_000818"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IYF54_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ9831168.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IYF54_000819"
     CDS             48806..49252
                     /locus_tag="IYF54_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ9831169.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IYF54_000820"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IYF54_000820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ9831170.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(51667..52641)
                     /locus_tag="IYF54_000821"
     CDS             complement(51667..52641)
                     /locus_tag="IYF54_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ9831171.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(52977..53064)
                     /locus_tag="IYF54_000822"
     tRNA            complement(52977..53064)
                     /locus_tag="IYF54_000822"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53030..53032),aa:Ser,seq:cga)
     gene            complement(53209..53775)
                     /locus_tag="IYF54_000823"
     CDS             complement(53209..53775)
                     /locus_tag="IYF54_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831172.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            54030..55361
                     /locus_tag="IYF54_000824"
     CDS             54030..55361
                     /locus_tag="IYF54_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ9831173.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            55327..55677
                     /locus_tag="IYF54_000825"
     CDS             55327..55677
                     /locus_tag="IYF54_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831174.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(55769..56188)
                     /locus_tag="IYF54_000826"
     CDS             complement(55769..56188)
                     /locus_tag="IYF54_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ9831175.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(56189..56533)
                     /locus_tag="IYF54_000827"
     CDS             complement(56189..56533)
                     /locus_tag="IYF54_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ9831176.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            56883..57479
                     /locus_tag="IYF54_000828"
     CDS             56883..57479
                     /locus_tag="IYF54_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9831177.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        16181 a        12116 c        10052 g        19167 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    51661 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    51721 atgacgatcc aagtcttctt tatttatatt tacagcagta atttgactgt cttcatacgt
    51781 acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta
    51841 ttttccttct ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa
    51901 aaattgactg atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt
    51961 ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc
    52021 acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga
    52081 tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa
    52141 cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga
    52201 tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc
    52261 gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg
    52321 ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc
    52381 tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt
    52441 agcctcataa tagagtggat agttggatac cccagctgct attcctatca ttgcataatg
    52501 tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac
    52561 tacttcatta taagacatct cataatcaaa attccttcca aaataatggt ctttcgttgc
    52621 ataagtgatt gcggtacaca tcgttttccc tcctaaaaca aatcgttttt acacttttat
    52681 cttacatcta gtattttttt ttatcaaaaa aaaggccaaa gaagattgat ttactagcat
    52741 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    52801 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    52861 ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg
    52921 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    52981 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
    53041 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
    53101 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
    53161 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
    53221 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
    53281 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
    53341 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
    53401 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
    53461 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
    53521 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
    53581 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
    53641 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
    53701 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
    53761 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
    53821 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
    53881 tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
    53941 ccttacattt ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata
    54001 gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc
    54061 atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg
    54121 ttttttttct ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc
    54181 taactattct tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg
    54241 gaagcgcatt cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc
    54301 acaaggtgat tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt
    54361 tttttggcaa aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg
    54421 tatttgtcaa taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat
    54481 caccggtcgt tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat
    54541 tggggaattt agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt
    54601 tagttttcca tcgtggattc acactttata atattggatt cacctctggg ttgattgcga
    54661 tgacttttac tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt
    54721 tcaatgaata tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg
    54781 gaattggttt ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat
    54841 ctggaaaatt gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca
    54901 tgggcttagt tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg
    54961 gacctgtcat cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa
    55021 aaaacagttt tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg
    55081 agatcaccag tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa
    55141 gcggttttta cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc
    55201 ataacgtcag cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt
    55261 tcgttgcagg tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa
    55321 agacacttgg aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat
    55381 tcccatgaaa taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata
    55441 gagatacagg gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg
    55501 aacaatcctc gggaccctag tatggaaatc tactatcaag aactattagg taatccccga
    55561 catgatcggg aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat
    55621 gcccagccga ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc
    55681 tattttctat ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc
    55741 tactttcagg agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat
    55801 ctgttacagg ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac
    55861 tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag
    55921 ccattaatcg tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa
    55981 gtaagatttg atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag
    56041 ttacattacc tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt
    56101 gcccggtaaa tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat
    56161 tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt
    56221 ctaaatagat ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa
    56281 aagctaaatg ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa
    56341 tcttcagatg agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga
    56401 tgatactacg ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat
    56461 catcatttaa ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt
    56521 gtttttctgc catttctctt attctccctt cacgactgtt aggtgattca actatggttc
    56581 taaggcaact ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc
    56641 tgtccaatgc ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt
    56701 ttattttaaa aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa
    56761 tcttttatac ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc
    56821 gcagttagtt gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa
    56881 taatgaatat tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc
    56941 tatcaatcgc gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt
    57001 taagaggatt agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa
    57061 tcctgcaagt agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga
    57121 tatttggccc ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta
    57181 tcatttttct gctagggcgt cgttatggaa agccattcat actttccatg attagcgata
    57241 agacatataa caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg
    57301 ctctagctat ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga
    57361 caaacatgtc agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt
    57421 tcttatatag tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat
    57481 atttattttg taaaattagt cattgattga caatgt
//
LOCUS       DACIWG010000014        55197 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.014, whole genome shotgun sequence.
ACCESSION   DACIWG010000014 DACIWG010000000
VERSION     DACIWG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.014"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            368..1471
                     /locus_tag="IYF54_000829"
     CDS             368..1471
                     /locus_tag="IYF54_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ9831178.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1755..2435
                     /locus_tag="IYF54_000830"
     CDS             1755..2435
                     /locus_tag="IYF54_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ9831179.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2525..3559
                     /locus_tag="IYF54_000831"
     CDS             2525..3559
                     /locus_tag="IYF54_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ9831180.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3573..3908
                     /locus_tag="IYF54_000832"
     CDS             3573..3908
                     /locus_tag="IYF54_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831181.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3892..6372
                     /locus_tag="IYF54_000833"
     CDS             3892..6372
                     /locus_tag="IYF54_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831182.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6428..7987
                     /locus_tag="IYF54_000834"
     CDS             6428..7987
                     /locus_tag="IYF54_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831183.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7984..9348
                     /locus_tag="IYF54_000835"
     CDS             7984..9348
                     /locus_tag="IYF54_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9831184.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9519..10589
                     /locus_tag="IYF54_000836"
     CDS             9519..10589
                     /locus_tag="IYF54_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831185.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10604..11272
                     /locus_tag="IYF54_000837"
     CDS             10604..11272
                     /locus_tag="IYF54_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831186.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11292..11870
                     /locus_tag="IYF54_000838"
     CDS             11292..11870
                     /locus_tag="IYF54_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831187.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12273..13184
                     /locus_tag="IYF54_000839"
     CDS             12273..13184
                     /locus_tag="IYF54_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9831188.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13440..14357)
                     /locus_tag="IYF54_000840"
     CDS             complement(13440..14357)
                     /locus_tag="IYF54_000840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ9831189.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14559..14996
                     /gene="zapA"
                     /locus_tag="IYF54_000841"
     CDS             14559..14996
                     /gene="zapA"
                     /locus_tag="IYF54_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ9831190.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15054..15602
                     /locus_tag="IYF54_000842"
     CDS             15054..15602
                     /locus_tag="IYF54_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ9831191.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15717..18077
                     /locus_tag="IYF54_000843"
     CDS             15717..18077
                     /locus_tag="IYF54_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ9831192.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18164..18478
                     /gene="trxA"
                     /locus_tag="IYF54_000844"
     CDS             18164..18478
                     /gene="trxA"
                     /locus_tag="IYF54_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ9831193.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18660..20438
                     /gene="uvrC"
                     /locus_tag="IYF54_000845"
     CDS             18660..20438
                     /gene="uvrC"
                     /locus_tag="IYF54_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ9831194.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20583..21482
                     /locus_tag="IYF54_000846"
     CDS             20583..21482
                     /locus_tag="IYF54_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831195.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21475..22737
                     /locus_tag="IYF54_000847"
     CDS             21475..22737
                     /locus_tag="IYF54_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831196.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22900..24798
                     /locus_tag="IYF54_000848"
     CDS             22900..24798
                     /locus_tag="IYF54_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ9831197.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25097..25816)
                     /locus_tag="IYF54_000849"
     CDS             complement(25097..25816)
                     /locus_tag="IYF54_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ9831198.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26153..26485
                     /locus_tag="IYF54_000850"
     CDS             26153..26485
                     /locus_tag="IYF54_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831199.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26472..28475
                     /locus_tag="IYF54_000851"
     CDS             26472..28475
                     /locus_tag="IYF54_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ9831200.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28494..28964
                     /locus_tag="IYF54_000852"
     CDS             28494..28964
                     /locus_tag="IYF54_000852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ9831201.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28985..29569
                     /locus_tag="IYF54_000853"
     CDS             28985..29569
                     /locus_tag="IYF54_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ9831202.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29584..30570
                     /locus_tag="IYF54_000854"
     CDS             29584..30570
                     /locus_tag="IYF54_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ9831203.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30560..30871
                     /locus_tag="IYF54_000855"
     CDS             30560..30871
                     /locus_tag="IYF54_000855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ9831204.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30893..32674
                     /locus_tag="IYF54_000856"
     CDS             30893..32674
                     /locus_tag="IYF54_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ9831205.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMCG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32667..34043
                     /locus_tag="IYF54_000857"
     CDS             32667..34043
                     /locus_tag="IYF54_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ9831206.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34050..34685
                     /locus_tag="IYF54_000858"
     CDS             34050..34685
                     /locus_tag="IYF54_000858"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ9831207.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34715..36070
                     /locus_tag="IYF54_000859"
     CDS             34715..36070
                     /locus_tag="IYF54_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ9831208.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36262..37656)
                     /locus_tag="IYF54_000860"
     CDS             complement(36262..37656)
                     /locus_tag="IYF54_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ9831209.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37748..38719
                     /locus_tag="IYF54_000861"
     CDS             37748..38719
                     /locus_tag="IYF54_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ9831210.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38716..39498
                     /locus_tag="IYF54_000862"
     CDS             38716..39498
                     /locus_tag="IYF54_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ9831211.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39667..40038)
                     /locus_tag="IYF54_000863"
     CDS             complement(39667..40038)
                     /locus_tag="IYF54_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ9831212.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40172..40615
                     /locus_tag="IYF54_000864"
     CDS             40172..40615
                     /locus_tag="IYF54_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ9831213.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="IYF54_000865"
     CDS             complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="IYF54_000865"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ9831214.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41486..42853)
                     /locus_tag="IYF54_000866"
     CDS             complement(41486..42853)
                     /locus_tag="IYF54_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9831215.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42837..43667)
                     /locus_tag="IYF54_000867"
     CDS             complement(42837..43667)
                     /locus_tag="IYF54_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ9831216.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43830..44267
                     /locus_tag="IYF54_000868"
     CDS             43830..44267
                     /locus_tag="IYF54_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ9831217.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44343..44963
                     /locus_tag="IYF54_000869"
     CDS             44343..44963
                     /locus_tag="IYF54_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ9831218.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45152..45658
                     /locus_tag="IYF54_000870"
     CDS             45152..45658
                     /locus_tag="IYF54_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ9831219.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45675..46574
                     /locus_tag="IYF54_000871"
     CDS             45675..46574
                     /locus_tag="IYF54_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ9831220.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46661..47545
                     /locus_tag="IYF54_000872"
     CDS             46661..47545
                     /locus_tag="IYF54_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ9831221.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47737..47808)
                     /locus_tag="IYF54_000873"
     tRNA            complement(47737..47808)
                     /locus_tag="IYF54_000873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47773..47775),aa:Arg,seq:ccg)
     gene            47868..49202
                     /gene="rpoN"
                     /locus_tag="IYF54_000874"
     CDS             47868..49202
                     /gene="rpoN"
                     /locus_tag="IYF54_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ9831222.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49405..50442
                     /locus_tag="IYF54_000875"
     CDS             49405..50442
                     /locus_tag="IYF54_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ9831223.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50483..51484
                     /gene="gap"
                     /locus_tag="IYF54_000876"
     CDS             50483..51484
                     /gene="gap"
                     /locus_tag="IYF54_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ9831224.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51594..52784
                     /locus_tag="IYF54_000877"
     CDS             51594..52784
                     /locus_tag="IYF54_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ9831225.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52888..53643
                     /locus_tag="IYF54_000878"
     CDS             52888..53643
                     /locus_tag="IYF54_000878"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ9831226.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53759..55057
                     /gene="eno"
                     /locus_tag="IYF54_000879"
     CDS             53759..55057
                     /gene="eno"
                     /locus_tag="IYF54_000879"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ9831227.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18305 a         9607 c        11686 g        15599 t
ORIGIN      
        1 ataccgaggg tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag
       61 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac
      121 ttaataccga gaaaatggct tttgaacaca gtttattcaa aaaaagggac tttgacacta
      181 cttttgtcaa agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt
      241 agtgacatct ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag
      301 taatataaaa agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg
      361 cattgagatg gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa
      421 tatggaagat ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat
      481 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg
      541 tttgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
      601 cgaagaaatg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca
      661 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
      721 aagttcttac gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc
      781 gcaatggttt caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt
      841 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
      901 ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg ccaatgccga tcgttcaggc
      961 ttatcaatca ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact
     1021 tagtccaaaa gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg
     1081 tgttcaatca gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt
     1141 atctaaccat ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata
     1201 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg
     1261 acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt
     1321 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
     1381 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
     1441 aataaaattg cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc
     1501 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
     1561 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
     1621 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
     1681 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
     1741 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
     1801 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
     1861 cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag
     1921 tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg
     1981 tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
     2041 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
     2101 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
     2161 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
     2221 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
     2281 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
     2341 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
     2401 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
     2461 cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg
     2521 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
     2581 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
     2641 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
     2701 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
     2761 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
     2821 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
     2881 ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc
     2941 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
     3001 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
     3061 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
     3121 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
     3181 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
     3241 tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
     3301 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
     3361 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
     3421 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
     3481 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
     3541 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
     3601 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
     3661 caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg
     3721 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
     3781 tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat
     3841 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
     3901 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
     3961 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
     4021 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
     4081 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
     4141 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
     4201 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
     4261 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
     4321 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
     4381 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
     4441 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
     4501 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
     4561 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
     4621 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
     4681 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
     4741 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
     4801 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
     4861 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
     4921 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
     4981 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
     5041 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
     5101 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
     5161 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
     5221 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
     5281 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
     5341 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
     5401 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
     5461 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
     5521 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
     5581 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
     5641 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
     5701 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
     5761 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
     5821 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
     5881 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
     5941 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
     6001 cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg
     6061 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
     6121 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
     6181 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
     6241 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
     6301 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
     6361 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
     6421 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
     6481 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
     6541 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
     6601 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
     6661 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
     6721 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
     6781 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
     6841 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
     6901 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
     6961 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
     7021 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
     7081 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
     7141 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
     7201 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
     7261 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
     7321 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
     7381 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
     7441 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
     7501 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
     7561 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
     7621 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
     7681 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
     7741 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
     7801 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
     7861 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
     7921 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
     7981 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
     8041 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
     8101 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
     8161 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
     8221 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
     8281 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
     8341 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
     8401 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
     8461 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
     8521 aatgttttaa caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
     8581 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
     8641 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
     8701 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
     8761 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
     8821 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
     8881 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
     8941 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
     9001 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
     9061 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
     9121 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
     9181 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
     9241 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
     9301 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
     9361 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
     9421 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
     9481 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
     9541 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
     9601 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
     9661 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
     9721 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
     9781 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
     9841 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
     9901 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
     9961 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    10021 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
    10081 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    10141 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    10201 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    10261 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    10321 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    10381 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    10441 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    10501 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    10561 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
    10621 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    10681 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    10741 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    10801 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
    10861 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    10921 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
    10981 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    11041 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    11101 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    11161 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    11221 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    11281 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    11341 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
    11401 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
    11461 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    11521 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    11581 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    11641 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    11701 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    11761 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    11821 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    11881 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
    11941 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
    12001 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
    12061 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
    12121 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
    12181 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
    12241 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
    12301 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
    12361 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
    12421 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
    12481 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
    12541 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
    12601 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
    12661 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
    12721 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
    12781 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
    12841 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
    12901 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
    12961 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
    13021 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
    13081 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
    13141 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
    13201 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
    13261 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
    13321 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
    13381 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
    13441 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
    13501 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
    13561 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
    13621 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
    13681 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
    13741 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
    13801 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
    13861 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
    13921 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    13981 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    14041 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    14101 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    14161 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    14221 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    14281 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    14341 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    14401 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    14461 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    14521 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    14581 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    14641 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    14701 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    14761 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    14821 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    14881 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    14941 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    15001 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    15061 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    15121 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    15181 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    15241 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    15301 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    15361 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    15421 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    15481 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
    15541 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    15601 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc
    15661 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    15721 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    15781 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa
    15841 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    15901 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag
    15961 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    16021 tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt
    16081 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    16141 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    16201 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    16261 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    16321 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    16381 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    16441 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    16501 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    16561 cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg
    16621 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    16681 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    16741 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    16801 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    16861 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    16921 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    16981 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    17041 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata
    17101 cgattaatgc cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga
    17161 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    17221 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    17281 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    17341 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    17401 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg
    17461 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    17521 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    17581 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    17641 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    17701 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    17761 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    17821 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    17881 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    17941 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    18001 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    18061 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    18121 aatgctatac taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    18181 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    18241 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    18301 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    18361 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    18421 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    18481 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    18541 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    18601 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    18661 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    18721 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    18781 tccgctcata ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg
    18841 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    18901 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    18961 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag
    19021 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    19081 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    19141 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    19201 atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt
    19261 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    19321 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    19381 ctgatttact ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc
    19441 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    19501 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    19561 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    19621 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    19681 cgaataagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    19741 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    19801 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    19861 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    19921 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga
    19981 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    20041 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    20101 caaaaaacga taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac
    20161 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    20221 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    20281 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    20341 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    20401 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    20461 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    20521 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    20581 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    20641 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    20701 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    20761 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    20821 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    20881 ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa
    20941 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    21001 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    21061 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    21121 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    21181 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    21241 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    21301 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    21361 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    21421 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    21481 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    21541 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    21601 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    21661 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    21721 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    21781 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    21841 atacaacaac aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca
    21901 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    21961 gattcaaaag gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt
    22021 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    22081 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    22141 caaaagcatt tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca
    22201 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    22261 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    22321 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    22381 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    22441 ggatatatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    22501 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    22561 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    22621 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta
    22681 tggagttcat tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat
    22741 atagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    22801 ctatttctat ttttttatta tattggatat atatattcat tgaaaataga gcataacaac
    22861 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
    22921 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
    22981 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
    23041 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
    23101 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
    23161 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
    23221 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
    23281 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
    23341 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
    23401 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
    23461 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
    23521 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
    23581 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
    23641 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
    23701 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
    23761 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
    23821 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
    23881 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
    23941 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
    24001 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
    24061 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
    24121 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
    24181 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
    24241 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
    24301 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
    24361 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
    24421 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
    24481 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
    24541 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
    24601 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
    24661 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
    24721 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
    24781 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
    24841 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
    24901 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
    24961 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
    25021 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    25081 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
    25141 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
    25201 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
    25261 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
    25321 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
    25381 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
    25441 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
    25501 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
    25561 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
    25621 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
    25681 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
    25741 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
    25801 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
    25861 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    25921 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    25981 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    26041 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    26101 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    26161 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
    26221 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
    26281 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
    26341 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    26401 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
    26461 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    26521 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
    26581 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
    26641 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
    26701 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    26761 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
    26821 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
    26881 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    26941 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    27001 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    27061 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
    27121 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
    27181 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
    27241 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    27301 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
    27361 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
    27421 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    27481 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
    27541 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
    27601 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
    27661 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    27721 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    27781 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    27841 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    27901 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    27961 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    28021 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    28081 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    28141 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    28201 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    28261 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    28321 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    28381 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    28441 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    28501 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    28561 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    28621 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    28681 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    28741 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    28801 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    28861 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    28921 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    28981 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    29041 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    29101 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    29161 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    29221 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    29281 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    29341 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    29401 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    29461 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    29521 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    29581 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    29641 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    29701 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    29761 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    29821 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    29881 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    29941 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    30001 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    30061 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    30121 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    30181 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    30241 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    30301 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    30361 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    30421 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    30481 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    30541 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    30601 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    30661 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    30721 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    30781 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    30841 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    30901 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    30961 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgtg
    31021 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    31081 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    31141 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    31201 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    31261 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    31321 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    31381 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    31441 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    31501 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    31561 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    31621 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    31681 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    31741 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    31801 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    31861 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    31921 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    31981 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    32041 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    32101 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    32161 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    32221 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    32281 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    32341 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    32401 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    32461 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    32521 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    32581 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    32641 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    32701 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    32761 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    32821 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    32881 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    32941 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    33001 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    33061 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    33121 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    33181 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    33241 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    33301 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    33361 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    33421 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    33481 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    33541 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    33601 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    33661 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    33721 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    33781 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    33841 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    33901 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    33961 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    34021 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    34081 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    34141 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    34201 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    34261 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    34321 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    34381 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    34441 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    34501 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    34561 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    34621 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    34681 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    34741 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    34801 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    34861 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    34921 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    34981 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    35041 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    35101 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    35161 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    35221 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    35281 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    35341 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    35401 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    35461 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    35521 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    35581 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    35641 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    35701 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    35761 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    35821 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    35881 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    35941 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    36001 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    36061 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    36121 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    36181 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    36241 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    36301 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    36361 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    36421 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    36481 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    36541 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    36601 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    36661 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    36721 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    36781 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    36841 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    36901 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    36961 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    37021 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    37081 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    37141 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    37201 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    37261 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    37321 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    37381 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    37441 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    37501 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    37561 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    37621 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    37681 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    37741 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    37801 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    37861 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    37921 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    37981 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    38041 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    38101 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    38161 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    38221 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    38281 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    38341 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    38401 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    38461 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    38521 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    38581 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    38641 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    38701 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    38761 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    38821 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    38881 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    38941 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    39001 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    39061 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    39121 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    39181 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    39241 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    39301 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    39361 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    39421 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    39481 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    39541 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    39601 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    39661 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    39721 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    39781 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    39841 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    39901 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    39961 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    40021 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    40081 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    40141 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    40201 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    40261 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    40321 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    40381 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    40441 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    40501 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    40561 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    40621 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    40681 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    40741 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    40801 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    40861 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    40921 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    40981 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    41041 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    41101 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    41161 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    41221 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    41281 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    41341 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    41401 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    41461 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    41521 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    41581 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    41641 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    41701 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    41761 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    41821 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    41881 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    41941 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    42001 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    42061 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    42121 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    42181 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    42241 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    42301 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    42361 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    42421 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    42481 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    42541 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    42601 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    42661 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    42721 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    42781 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    42841 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    42901 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    42961 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    43021 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    43081 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    43141 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    43201 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    43261 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    43321 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    43381 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    43441 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    43501 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    43561 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    43621 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    43681 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    43741 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    43801 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    43861 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    43921 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    43981 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    44041 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    44101 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    44161 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    44221 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    44281 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    44341 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    44401 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    44461 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    44521 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    44581 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    44641 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    44701 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    44761 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    44821 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    44881 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    44941 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    45001 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    45061 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    45121 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    45181 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    45241 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    45301 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    45361 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    45421 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    45481 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    45541 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    45601 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    45661 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    45721 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    45781 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    45841 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    45901 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    45961 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    46021 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    46081 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    46141 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    46201 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    46261 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    46321 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    46381 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    46441 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    46501 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    46561 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    46621 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    46681 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    46741 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    46801 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    46861 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    46921 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    46981 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    47041 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    47101 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    47161 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    47221 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    47281 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    47341 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    47401 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    47461 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    47521 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    47581 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    47641 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    47701 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    47761 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    47821 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    47881 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    47941 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    48001 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    48061 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    48121 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    48181 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    48241 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    48301 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    48361 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    48421 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    48481 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    48541 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    48601 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    48661 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    48721 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    48781 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    48841 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    48901 gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat
    48961 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    49021 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg
    49081 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    49141 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    49201 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    49261 agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    49321 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    49381 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    49441 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    49501 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    49561 acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    49621 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    49681 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    49741 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    49801 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    49861 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    49921 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    49981 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    50041 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    50101 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    50161 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    50221 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    50281 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    50341 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    50401 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    50461 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    50521 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    50581 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    50641 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    50701 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    50761 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    50821 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    50881 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    50941 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    51001 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    51061 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    51121 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    51181 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    51241 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    51301 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    51361 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    51421 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    51481 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    51541 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    51601 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    51661 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    51721 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    51781 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    51841 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    51901 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    51961 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    52021 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    52081 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    52141 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    52201 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    52261 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    52321 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    52381 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    52441 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    52501 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    52561 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    52621 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    52681 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    52741 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    52801 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    52861 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    52921 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    52981 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    53041 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    53101 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    53161 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    53221 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    53281 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    53341 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    53401 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    53461 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    53521 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    53581 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    53641 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    53701 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    53761 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    53821 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    53881 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    53941 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    54001 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    54061 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    54121 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    54181 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    54241 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    54301 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    54361 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    54421 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    54481 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    54541 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    54601 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    54661 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    54721 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    54781 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    54841 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    54901 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    54961 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    55021 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    55081 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    55141 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgttt
//
LOCUS       DACIWG010000015        54992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.015, whole genome shotgun sequence.
ACCESSION   DACIWG010000015 DACIWG010000000
VERSION     DACIWG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.015"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            4..1086
                     /locus_tag="IYF54_000880"
     CDS             4..1086
                     /locus_tag="IYF54_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9831228.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="IYF54_000881"
     CDS             complement(1304..2446)
                     /locus_tag="IYF54_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9831229.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="IYF54_000882"
     CDS             complement(2451..2636)
                     /locus_tag="IYF54_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9831230.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="IYF54_000883"
     CDS             complement(2990..3235)
                     /locus_tag="IYF54_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831231.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="IYF54_000884"
     CDS             complement(3229..3630)
                     /locus_tag="IYF54_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9831232.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYF54_000885"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYF54_000885"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ9831233.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="IYF54_000886"
     CDS             complement(5648..7414)
                     /locus_tag="IYF54_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ9831234.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7427..8221)
                     /locus_tag="IYF54_000887"
     CDS             complement(7427..8221)
                     /locus_tag="IYF54_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9831235.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8231..9607)
                     /locus_tag="IYF54_000888"
     CDS             complement(8231..9607)
                     /locus_tag="IYF54_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9831236.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVNWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYF54_000889"
     CDS             complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYF54_000889"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9831237.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYF54_000890"
     CDS             complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYF54_000890"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ9831238.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYF54_000891"
     CDS             complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYF54_000891"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9831239.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13041..14672)
                     /locus_tag="IYF54_000892"
     CDS             complement(13041..14672)
                     /locus_tag="IYF54_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ9831240.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14976..15647
                     /locus_tag="IYF54_000893"
     CDS             14976..15647
                     /locus_tag="IYF54_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9831241.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16372..16707)
                     /locus_tag="IYF54_000894"
                     /pseudo
     CDS             complement(16372..16707)
                     /locus_tag="IYF54_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16804..17256)
                     /locus_tag="IYF54_000895"
     CDS             complement(16804..17256)
                     /locus_tag="IYF54_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831242.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17263..17811)
                     /locus_tag="IYF54_000896"
     CDS             complement(17263..17811)
                     /locus_tag="IYF54_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9831243.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17856..18188)
                     /locus_tag="IYF54_000897"
     CDS             complement(17856..18188)
                     /locus_tag="IYF54_000897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831244.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18202..18432)
                     /locus_tag="IYF54_000898"
     CDS             complement(18202..18432)
                     /locus_tag="IYF54_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831245.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18654..19196)
                     /locus_tag="IYF54_000899"
     CDS             complement(18654..19196)
                     /locus_tag="IYF54_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831246.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19217..20308)
                     /locus_tag="IYF54_000900"
     CDS             complement(19217..20308)
                     /locus_tag="IYF54_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ9831247.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20316..22526)
                     /locus_tag="IYF54_000901"
     CDS             complement(20316..22526)
                     /locus_tag="IYF54_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831248.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22542..25070)
                     /locus_tag="IYF54_000902"
     CDS             complement(22542..25070)
                     /locus_tag="IYF54_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ9831249.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25122..25532)
                     /locus_tag="IYF54_000903"
     CDS             complement(25122..25532)
                     /locus_tag="IYF54_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831250.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25545..25763)
                     /locus_tag="IYF54_000904"
     CDS             complement(25545..25763)
                     /locus_tag="IYF54_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831251.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25760..26752)
                     /locus_tag="IYF54_000905"
     CDS             complement(25760..26752)
                     /locus_tag="IYF54_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9831252.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26831..27742)
                     /locus_tag="IYF54_000906"
     CDS             complement(26831..27742)
                     /locus_tag="IYF54_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9831253.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27761..28105)
                     /locus_tag="IYF54_000907"
     CDS             complement(27761..28105)
                     /locus_tag="IYF54_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831254.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28125..28681)
                     /locus_tag="IYF54_000908"
                     /pseudo
     CDS             complement(28125..28681)
                     /locus_tag="IYF54_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28762..29262)
                     /locus_tag="IYF54_000909"
     CDS             complement(28762..29262)
                     /locus_tag="IYF54_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831255.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29263..29808)
                     /locus_tag="IYF54_000910"
     CDS             complement(29263..29808)
                     /locus_tag="IYF54_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831256.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29805..31061)
                     /locus_tag="IYF54_000911"
     CDS             complement(29805..31061)
                     /locus_tag="IYF54_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ9831257.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31422..31733)
                     /locus_tag="IYF54_000912"
     CDS             complement(31422..31733)
                     /locus_tag="IYF54_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831258.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31756..33066)
                     /locus_tag="IYF54_000913"
     CDS             complement(31756..33066)
                     /locus_tag="IYF54_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ9831259.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33680..34132)
                     /locus_tag="IYF54_000914"
     CDS             complement(33680..34132)
                     /locus_tag="IYF54_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831260.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34134..34499)
                     /locus_tag="IYF54_000915"
     CDS             complement(34134..34499)
                     /locus_tag="IYF54_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831261.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34598..38881)
                     /locus_tag="IYF54_000916"
     CDS             complement(34598..38881)
                     /locus_tag="IYF54_000916"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ9831262.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39028..39267)
                     /locus_tag="IYF54_000917"
     CDS             complement(39028..39267)
                     /locus_tag="IYF54_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831263.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39357..39629)
                     /locus_tag="IYF54_000918"
     CDS             complement(39357..39629)
                     /locus_tag="IYF54_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831264.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39712..39963)
                     /locus_tag="IYF54_000919"
     CDS             complement(39712..39963)
                     /locus_tag="IYF54_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831265.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40959..41870)
                     /locus_tag="IYF54_000920"
     CDS             complement(40959..41870)
                     /locus_tag="IYF54_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831266.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="IYF54_000921"
     CDS             complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="IYF54_000921"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ9831267.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44048..45268
                     /locus_tag="IYF54_000922"
     CDS             44048..45268
                     /locus_tag="IYF54_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831268.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45288..45782
                     /locus_tag="IYF54_000923"
     CDS             45288..45782
                     /locus_tag="IYF54_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ9831269.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45775..46446
                     /locus_tag="IYF54_000924"
     CDS             45775..46446
                     /locus_tag="IYF54_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831270.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="IYF54_000925"
     CDS             complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="IYF54_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ9831271.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49457..50503)
                     /locus_tag="IYF54_000926"
     CDS             complement(49457..50503)
                     /locus_tag="IYF54_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ9831272.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50514..50786)
                     /locus_tag="IYF54_000927"
     CDS             complement(50514..50786)
                     /locus_tag="IYF54_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ9831273.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50821..51729)
                     /locus_tag="IYF54_000928"
     CDS             complement(50821..51729)
                     /locus_tag="IYF54_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ9831274.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="IYF54_000929"
     CDS             complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="IYF54_000929"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ9831275.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53108..54229)
                     /locus_tag="IYF54_000930"
     CDS             complement(53108..54229)
                     /locus_tag="IYF54_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ9831276.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54226..54927)
                     /locus_tag="IYF54_000931"
     CDS             complement(54226..54927)
                     /locus_tag="IYF54_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ9831277.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT        16578 a        11367 c         8237 g        18810 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac
     7501 atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa
     7561 taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa
     7621 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt
     7681 tttttccttt atcttgacaa cacagttcat caactaatga taataattca tgtctttccc
     7741 attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
     7801 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac
     7861 taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
     7921 aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt
     7981 gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa
     8041 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
     8101 tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt
     8161 tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca
     8221 tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt
     8281 tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa
     8341 ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag
     8401 agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta
     8461 tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta
     8521 cttcttgatc atcaataaca tcagtaatat ttgcccaatt aacagtatta aacacaccca
     8581 aacccatata gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca
     8641 aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt
     8701 ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag
     8761 gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat
     8821 aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta
     8881 tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt
     8941 attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa
     9001 taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta
     9061 tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca
     9121 aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa
     9181 tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac
     9241 tcccccataa tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa
     9301 tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa
     9361 tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag
     9421 ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc
     9481 cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag
     9541 taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc
     9601 tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac
     9661 tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt
     9721 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctactcgact
     9781 tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc
     9841 aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat
     9901 tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga
     9961 acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat
    10021 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg
    10081 atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa
    10141 cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc
    10201 aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat
    10261 tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac
    10321 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
    10381 ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag aataaattta
    10441 agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac
    10501 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
    10561 cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg
    10621 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
    10681 ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
    10741 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
    10801 gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca
    10861 ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
    10921 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
    10981 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
    11041 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
    11101 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
    11161 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc
    11221 aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt
    11281 aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta
    11341 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct
    11401 gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg
    11461 cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc
    11521 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
    11581 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
    11641 tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta
    11701 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
    11761 ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt
    11821 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
    11881 aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa
    11941 ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
    12001 taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc
    12061 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
    12121 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa
    12181 atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
    12241 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
    12301 tccagtacat aatgtaatac cattagaagg agaatccact acattacaca accaatctaa
    12361 atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg
    12421 gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
    12481 tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt
    12541 aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
    12601 tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt
    12661 agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat
    12721 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
    12781 attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac
    12841 actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat
    12901 tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt
    12961 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
    13021 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
    13081 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct
    13141 accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc
    13201 gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc
    13261 aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc
    13321 agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
    13381 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
    13441 gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta
    13501 attacttcat caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa
    13561 ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct
    13621 gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc
    13681 aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc
    13741 acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa
    13801 tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata
    13861 tctaaatcct cgagccccat catcaataga tcttccttta cattctctga aggatttggc
    13921 gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
    13981 ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta
    14041 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac
    14101 tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga
    14161 ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct
    14221 tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca
    14281 aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata taaactatca
    14341 gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa
    14401 atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac
    14461 acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
    14521 aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct
    14581 tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg
    14641 atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag
    14701 ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata
    14761 aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac
    14821 tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt
    14881 atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa
    14941 atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa
    15001 atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa
    15061 aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag
    15121 cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata
    15181 tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac
    15241 gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa
    15301 ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata
    15361 atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat
    15421 ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag
    15481 gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac
    15541 aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc
    15601 aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt
    15661 ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata
    15721 tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt
    15781 atgactgctg ataacttttg atattaaact agtatctcca atacaaataa aaaatagata
    15841 tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc
    15901 ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact
    15961 ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg
    16021 ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg cttttttcaa
    16081 aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac
    16141 atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact tataagaaat
    16201 tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca
    16261 gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc
    16321 cgattactta tattctacgt atagttttag tattcttatt ttttatcacc agtaaccaat
    16381 tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt caggcttagg
    16441 aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag aaataccact
    16501 gcctatcttt tttacaaata tttcatttgg tgtacagact aataaaacaa tatttgtaat
    16561 caatccttgc actaaagaat taaaaggaag actatttaaa acagggtgtg cataactata
    16621 acatgtgaag tagttatttt gaactttaac ttttaaaaac tttaaaaata atcttatttg
    16681 ttcttctaaa caatcatcta tactcatata atcactccac taattaatct ccccaaaaag
    16741 acacagcatt aagagatata ccaacacaac agtgcaaata actctacatc ttccttcgca
    16801 accttatttc aataaaccat aaaaattatt ttttaataga aactgaaaat taaaactaga
    16861 aaaagtagtt ttaccgaatg taagagcatc atcaccatca atatagaaat acattctttt
    16921 ccagttacat ttaattttta aacaaaattc tccccaaaaa ggaacttttt ctagtgaaaa
    16981 cttggtaatc tctttgtaat taattcttgt taaagaataa ttaaacactt catttttact
    17041 tgttattttt tcattgacta taactagttc atctggtaaa aaaattaacc aacaaacatc
    17101 gacaaacagt aaatgatata atccatttcc acctccaagc atattcaata ctggtgaaca
    17161 tgccttagaa acacgaatat agttatggtt taaatagata ttttctttaa acattgcttg
    17221 tttaaaagcc atatgctctt gttctaaact tttcataaac ttttactcta atgcacagct
    17281 agcagcagct gctgcaattc ctgctacaat atttacctta gctaactctt tagctgcagc
    17341 ttttcctaat tgtttttctg caatttttaa taataaatcc gcagcttttt caaaattaaa
    17401 agcttttaat gcttcaataa aatctccctg cgtacctata gcccaagcca ctcctcctcc
    17461 aggaagagaa ttaatggcta cacatccagc atatcgtttc caatcaactt tctccattct
    17521 aggaacaata atatgttcat aaaagactct tgctgctctt ccttgttcgt tatcttgact
    17581 gattcgttca cgaatagcac caaaatcctt tatatggtat acaccatgtt cgtctaaaaa
    17641 accaactgta tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc
    17701 aactgtattc actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa
    17761 acctaataag aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc
    17821 tccataaaaa ataagttact ttttaaatat cttgtttatg aattcattcc ccaaaggcct
    17881 tttaagattg atacagcagt ccgttcactt cttttagact tgtcatacaa gtcttttaaa
    17941 tgatcccata tcagactagt ttgtctatct agcttcaatc cattaaacgc taatgaacgg
    18001 ttcatttttt ctattacttt agataaagct acagaaattt ttataccttc acaacgcgca
    18061 tcttggattt caataatagt atcatcaatt agtttttgaa tttcttgcct atcaacatat
    18121 tcaatcttag aaagagaatc ttttaaagct actaatgatt caacgatttc ttttttctct
    18181 tcagacattt tcttcctcct tctagtgacc cgtaaagcca tgtagagcag agtccattga
    18241 accagtaatt acactatcta caacgccatt ccaatttact ctacattttc catttttcag
    18301 atttgtcggt ccatacggat agcctttttg acattcaatg ttcggattat accagatagg
    18361 attataccat tcagccgcag aagcagattc agtcgataca aacaatccca caccaacaaa
    18421 tccaatacac atcacactat agccaattag ttttttcatc tcgtatccat ctcctaaaaa
    18481 atagaatagt tcttatagtt attcataaga catatttttt ccgtttttgt tttatccttt
    18541 ctgtcctata acaacttttt tcaagtaaag aatgacatag tttttcttaa aaaaacagtc
    18601 tgtttttttc tatttcttcg gtgtataata aatatgagtg ttcatttttt attttaatac
    18661 gcactccgta cataataaat cttgatcgca tcaatctttt gagcttcttg atctactttc
    18721 aattgaagta atacaaattg agtaacctta ttcattgttt cttctccaaa taacaaatat
    18781 tgatccggat tctctatatc ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt
    18841 acatgcgggt ctacatcaat actattgtct ttaatacaac gatctgtcat atagtccctc
    18901 acagcttgat tgcgtttata aacatctgtg aaattactcc attttctagc aaaatctttt
    18961 aagaaatttt ttgtcatttc atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa
    19021 ccaatttgac tgttactttc agtactttga agagttccac ttgtttcttt tactgcttcg
    19081 attgtttctt ctgtcgttct caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa
    19141 ccagtaagac ctatcaaaca taaccaacta aatactaaaa ttatcccttt tttcacgttt
    19201 ctagtcactg cctttcttat ttcaccttac gaaccagact acgaattttt gtttgattgt
    19261 acgtgcgttg ataccgagcc ataatccgtc cttgctcggc attctgttcc acactagaga
    19321 aatgttgtcc gccatatgaa acatctgtta taattccggt gtgaccatag attccaggag
    19381 acaatgcacc acctgcttgc caattcacca catctcctgc ttgtaaatca cttggttttg
    19441 ggttcatgat tacttgccaa ccataggttg tccagttata atcttcccct attaacatcg
    19501 caaacatgtg tccagaaccc attaactgtg ggccacctaa ttgagataca taccatgccg
    19561 taccaccata acattcccca ttatttacgg atcgtccaat gatccgatcc aaaaccttcg
    19621 cattgccttt actttttcct gcaataagcg aagttttttt tgttggtagg ctattccaat
    19681 gcttgatcgc aaaagtatca tattccgtta agttatagga cgttatgata ctcattgctc
    19741 ctcgataata cggactccct tcggtaatac ctgctgccgc atagcctcca cgacctagaa
    19801 tcaataaaca acttccagga tctttattgc ccacacattg attaaagttg ctttggctcg
    19861 ccaccataaa gccataatag tcataacaag cttttaagtt cggaaacttc atatagaaac
    19921 caccttcaga accaccaatt ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc
    19981 aggtaatccc aaaaaagttc gtatcgtttc ttccagattg tgactgtcca taagctgatt
    20041 catagcctaa ttgagaaaga atcacactcg gtaataactg atactttaca gctgcaccaa
    20101 ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg
    20161 gtaatccttc ggtatattcc ccattaaaag cgacagctag acctgtagct tctcctccat
    20221 gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa
    20281 gaagacaacc tagacaccct ttcttcattt cttcctcatc ctttcttcga taatggtttt
    20341 tggactgttc gtccttttgg taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc
    20401 agtgttttag gatccttttc tattttttcc gattgctctg ttggcttatc taaagaagaa
    20461 aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt tactttcaga cgaacgttgc
    20521 cccggttgtt gcacagaacg atttcctgat agagtggcta ccgacgaatt aggaacagag
    20581 tattggttca ataactgctg cattgctgga taactcccat ttttcgcctg atttttcact
    20641 tggcgaatct gttgtgaaag agaaccagca gctgttaatt gtggcacggg tacttttcca
    20701 gaaggcaacg gaatcgattg accaacaaaa gcgatacctt tatttaacaa tggttttctt
    20761 tgattattcc cttgttgctt actcaattgt gccaatgatt gtacctgttg ttttggtctt
    20821 actgaattca tggacgaatt tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt
    20881 ttcgatttgg tagaagacat ttcaggctct accccatgat aacgaacatg acgaccacta
    20941 taaggcaagg cttgatttga acgcccacct agttcaaagg gattaaccac ttgcctccct
    21001 tttaccgatt cttgtggtgt acgtttttgt tgaaactttt gaaatccatg tatcgctgca
    21061 gaagcgagtg gattcgttgc tcgatacaca ttccctgcta atcctgcacc tttgattgcc
    21121 aaattttgtc cagcagttga tagttgacca ggcagttgtt tcatttgaga caaagaatga
    21181 cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag ctcctaaccc tagtttcttt
    21241 aataaatctt tccgttttac aatgagtaaa attgatagga gtgctttgac aaacatattt
    21301 cctaaaaaac caaaaacagt attcattgga attaaatcgt ctactatgtt atagatcaaa
    21361 aatgccacca aaataatcac actgtaaaaa gatttttgaa acatcaagcc caaaaaggac
    21421 tttaaggtat tcatgagcat ataatcaaat actggaacaa aagaagcgag taatgaaaaa
    21481 ggaatcacaa gtaataaaca caacaccaac atttgaaaga taaaacgaat catgccaatc
    21541 actacaagtg gtaatccaat acttagatta agcaatggca ccatagaacc aacgactgct
    21601 ttataaacag ctttagttgg ttgaatatag acacgataag ccttcttttt ccctctatct
    21661 tctcctgcag cagtggtcac ttttgtctca atatcagata attcttcact tgacgcttcc
    21721 ttggctagaa attcttgtgg ttcaatctct gcttgataca attcttctaa atttgttgtc
    21781 ccataattta acaatatata cggatcaaca acagccgctt tataataaag ttggcgcact
    21841 ttttgaatgg cttccttttg tgtcgttggc actaaaacac cattgacatc ttttgtcgcc
    21901 aattcatcta gactatttgt agcagaaaaa ataaagcctt ctacctccac ggacaaatca
    21961 tttaaccctt tggtaaaagc ttctccttta ctaggtacgt tactaccata gccaaaccaa
    22021 accattgaaa aaatgacaac gaagagaaat cgaataaaca ccatttttgc gtaatgtttc
    22081 cctctaaata tacgaagagt aaatagatat aacacataca agacgacaat ccccgcacca
    22141 ataggatcgt atatttttga ataaatagta tccgaaaaag tgaaaaactg gtgaattaac
    22201 tcgttcattg cagctccttc atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc
    22261 aatttactta aactaaagaa catattcgtc atcattttta gaaaatctgt gccatttgcc
    22321 ccaaataaca ttttgcctaa tgaaaattca ctatcagata aataagaaga aaaggtatca
    22381 atatgaaatt tcccaatctc atagatcgat tgatccacct gcttttctgc cgcatacacc
    22441 aatccaccaa caaaaagcag tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc
    22501 ttttccttat gctttatata agccattgtt tcacctcatt tctattgcat gattaacgta
    22561 tcctgttctt ctacttgaga gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt
    22621 tcttcaaaca aactgtgtac attgagtttt cctacacgtc catagggatc taacatccaa
    22681 cactgtcctt taatcatgtg gctcatttca gctaagttcg cttcatttac ttccaaacgt
    22741 aaatgattca aaatcttttc tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa
    22801 ttcccttttt ctttatctga acctaagtca ccaataaatt gtgtatctaa cactaattga
    22861 ttcatctggg aacgccccac acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa
    22921 ttattaaata cccatgcttc tgtaaaataa atcgctgttt tcttactact atctcgtttt
    22981 ccaaacattt cacaaaaacg acctaaacta atcatcaaac acaaagactt tcgatccgca
    23041 tctgtatata gttctggtcg gacattgtca tttggtaatt tcaaatcttg gatctctaaa
    23101 atcgttgttt tggcattaaa gtctaatccc gggttttctc cataagaaaa tcctaaacgt
    23161 aaaatcgaat cttctaccgt caagcgtaaa aactcaccat attctcgaac ctctttttgt
    23221 tccatttgtt ctaaatagtc gaccacatgc atcaagccaa cttgttgtcc ggcttctttt
    23281 tgaactaaaa cttcttggat tccctttaat accgcccctt ttaaaaggtg attggtacct
    23341 aacggactta actcatcaat catatcctgg gctacttgtt tcgcatctac tcgggataaa
    23401 caaacaattg gatctaatac accataatta cggggatttg tagcatctaa cgtgacataa
    23461 ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt tctgataata cgggtcttga
    23521 atgagtttcg taaaccattt gcgcttttct cctttgggat ccacaaataa actttgtaca
    23581 ttcataaacg aactatacaa aaagagcaag ccaactaaaa aggtcttccc tttaccggtt
    23641 tttccagtta ctgctacatg aggcgcatca aacaacgcac ctttgattcc ttccgcaatg
    23701 gctagaagat tcaaaaagac aaaacgattt gacgcataaa ttaattcttc taatggtgtt
    23761 ttttctcctc gagaaccacc atcccctaat gtatcttgcc gaccaatata ccagccggat
    23821 tccataccta attctgatac tgctgaaaac aaccattcgc caactgcttc tacagttgtt
    23881 cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta ataaacattt atagaacaat
    23941 tctcgttgat cattttttgc acggtggata tggataatcg tatgaagttg ttgaataacc
    24001 gttgcactat tttgacgaac ttcttcaacc gtttgaccgt atacaataaa acatgcgatc
    24061 caatcgacga caggttgccc attatctact cgttgcttta aatgttttac taatcctcga
    24121 acagttaata agcgttcact aggttcttca tcattttgaa ccaattcctt atgctctgtt
    24181 ttgaaccgtt ccgatagcca attcagtttt aaaccaatac ctccctgtcc tttcaagcgt
    24241 ggatagtgaa ttttcattcg caattctgct gggaaagaaa gatcctgagc aaattgaaat
    24301 agatcacaat ttctcatatt ttcaggtgtt tccccaatag gcaacaatgc taaataacta
    24361 ctccctaact catcttctaa ttgaagaagg cctttatgtt ccttcggtat acgtacatac
    24421 gttccttgat tgtctttcag tacttcccaa ttattttgtt cttctacttt gtgagccatt
    24481 cctcgaatat attgcagacg attgagatac aacagctctt tttttactaa gggacgaata
    24541 gaaaagctat aaagtaattg tgtcacttct tttacataag gatcactttc agaaaacaaa
    24601 gtttccaaat catctacttc taatccaaat aagcgtgcta aacttcctgc taaatctaac
    24661 cagattagat ttattacatc atgaaaactc tccaccgttg ctttttcttt gactttgatt
    24721 cctaaaataa atgaaggacg atagacttga ccttctcgtt ccaacaatgt cactgtttct
    24781 tttgcataat acgcaccaat tgcttgacta tcgggagaaa atccctttcc taatgtgtca
    24841 aacttcgtcc gtaaattcat tgttcgtgga tacatcacca actccacatc ttcataatct
    24901 gctaaaagca acagtaaacg agccatcatt tcttttattt cctgtgtctt ttttggtgtg
    24961 ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc agatacaatc ctctccattt
    25021 aacaaacaat ttttcttgaa tgcttttact ggttctttta caagcaccaa tgaatttcac
    25081 ctcccaataa aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca
    25141 tacttattca caaaaagtaa ttcttccaat gcattgtttg ataccttgtc ccagttataa
    25201 tacacacaat caccattcat catacgaatc atataacaaa accaatccca taaataaaaa
    25261 tgcacttttt ttccatcagg ttttagtttt gccagtgcta acacacataa ataggggagt
    25321 cctatataaa aaacaactcc tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa
    25381 cgtaaaaata aatattcaaa gattacgacc aacaaaaaag ttgctgcatt gactaatgca
    25441 actggaaatg gaagacgcca attgcttgta atctgttgta cgcgataagg ttccgcataa
    25501 acaatcgaat aatcataagg ttcccaatcc acttcactat cctcctaatc cataaataat
    25561 ttttcaatga ttttaccaaa cgcatcaaat acacgtgttg gatcatttaa gaagaaataa
    25621 actgcggcac ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt
    25681 cgcaatccca gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa
    25741 tctacaattc ccttcagtgt catttccgtt cttctccttt cctatccaac caattatgtg
    25801 ttaatttttc aacataatac tgatttgttt ttttcgtaat gacaatcgtc atttgttctt
    25861 tttgtaaaag tgacgtatca ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt
    25921 ttttcgtttc tttataacgc aatactttca tctctacttg atcaacttta gctgtttgat
    25981 tgagtccttc aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact
    26041 tatctaagaa atcttccaca aatccttcaa catctttcgc ttccgacgca tcaaccgttt
    26101 cgtataatgt gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg
    26161 taaaataagg ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga
    26221 gtacttgtgt gattgtcaca cttgtttctt tcttactctc tggattcatc gagcgaatat
    26281 cataagttac tttataacta gcagttggaa tggtttcaat ttcttttaat ccaataaacg
    26341 aagcagcagt taattttcga acacctgaaa cacttcctgg ttcttgaata aagttttgtg
    26401 cataaaactc tttttttaat gtttcttgac gttgttgttc ttgttcatga ttatcgaatg
    26461 tattcatata caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta
    26521 attctgaccg actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg
    26581 acacttgaac cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa
    26641 ataaaaccaa acaccaaatc catttcactg ctttttttga atggattttc ttttgcttcg
    26701 gtactttctc tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact
    26761 ccttctagta tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa
    26821 aatagtccat tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa
    26881 atccagcaat acttgtcaac actcctccta gaacagaaag tgcaacatgt atttcttcac
    26941 cagtctttgg taagactttt gtttttccat ctgctgtttt gatttcaata ttgccacttg
    27001 gaagtttctt tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta
    27061 atggaatacc atcttttaca ccaacaattg tttgatcacc tgctactttt aatggggtgg
    27121 ttggtgttgg aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc
    27181 cttgagaagt attcgtcgta atttcaccgc ttggggttac cgatactgtg acttcttttg
    27241 ctggaacaat aggtttattt tcttttggaa ttgcttctga tttttcaaca ggttgtttcg
    27301 gttcttcttt ttgttcttca ctggtagacg acatcgttgg ctgttccgta cttgacgaat
    27361 cagtattctt ttcagaagaa ctggttgttc ctgattgttc cggagttgtg actatacttg
    27421 aatccgttgt tgttgaacta tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg
    27481 attcaggaaa ctctggttct ggtgtcactg gctctttttc aggtttctct gactgcaatt
    27541 gttcaggttc tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctggta
    27601 atccttcccc tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg
    27661 cataaaccaa tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga
    27721 ctgctaatgt tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt
    27781 tcatagacgc cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta
    27841 acccaacata tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg
    27901 gatagtttgc aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt
    27961 cataacgata ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc
    28021 atcgttccat tcgtggatag agatagacac gatacacata gaacaatgcc ataccacata
    28081 ccaccaaaaa taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc
    28141 atcgaacatt cataccctct atagagattt gaaaaatcca tcatcaattg ttcatataaa
    28201 cgttgttgca aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga
    28261 tcttcttttt catagcatct tgtagctcta tgacgctcag cttgcaagac ataaccaaac
    28321 tctgcaccat atcgttctac tgttaataac tgttgactac gcaataatgc aatataacaa
    28381 tcacaaaaat gttcaaccga catttgattt ttctcacgtt tgtgaaatag gtattcttta
    28441 gaaagcacat tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt
    28501 tcatctgtga atttttccca taataaactg atttttattc gttcagcggc tagatagtct
    28561 tgttcgcgtg gataaaattc atcaatcgca caaagttttt tatcacctga ataaacaaat
    28621 ccatataatg cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca
    28681 taccatcttc tccttcctaa aaaaaataaa aaagcagaaa ccgatttggc ttctactttt
    28741 ctttcctttt atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatc
    28801 aatcggcaca agttcacgaa aacttggagc aattttggat agaatcactt ccccatcttg
    28861 taaaatacaa tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat
    28921 aaaatagtag cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc
    28981 atcatagaaa taagccacaa atcgcccttc aagttcatgt ttttcaatgg gatactcttc
    29041 tggtaacaat ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc
    29101 taaacgtctg tcagtatatg gtaactgacg aatcaacgat tctaatcgtt ctttatacag
    29161 atagaataat tggcgcaagc aatagtgtac cgcctctgga ttttctaata ctgttggatc
    29221 acgtgaccaa tacgcaccaa cataaatcat aatttttttc atttagacaa tcacaccagc
    29281 aatactaatg accgatccac aataggtaga acaagtggca tagcgaccaa tttcctctaa
    29341 ttctatctca atctcttcta aagaaagata tgtctgttct tctgttggct ggcgactcat
    29401 ttcccaagtg acagtcacca atgaaatttc ttccataaga ggtttttcgc tgctaagtgc
    29461 tgccacacag ttttcatata tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc
    29521 aggtattggc aatgaaaatc gataaaagcg aatgaattct agccttcctt tccttcggct
    29581 aaattccaac tcttcgagtt cttgttcgta tcccttacga acttctaata agcgtgcaat
    29641 ttcttgccag ttctcttttt cctgataacg tccagctttt accgcttgtt caatataagg
    29701 aactcgaaaa cgaaaataag acaaatcaac catcttttca tttaatgctt cgattgcagc
    29761 ctttaaatcc aacaggcgca gccattctgt tgtcgtatat gcattcatgc aattttccct
    29821 cctgacgttc ccatccattc tagatagttt tgttgctcgt catttagttc ggcattatca
    29881 atcccttctt gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa caactttaat
    29941 gtcggagcta cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc
    30001 tttactgtaa aatccaattc ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca
    30061 atccgtgcat cactttccag tctatcccaa acaataagtt ttgcattaat taaaccaacg
    30121 ccaatctctc ctaaacgttt cggtgaatca cctaaaataa atgcttctac tgcttgtgtt
    30181 gctttctcat tggctaaccg taactcatat cgattataaa taccatattt cgttaatgct
    30241 tctaattctg aaattttttc tcggttcgca atttcaaaac gtttctcata aaaattaaaa
    30301 aagagaggac tctttcgcga tccaaaatac aaagataatc cttcacttcg aaaatagcca
    30361 tcatctaaaa aacaaccgcc aatattctgg aatctcttaa agtccatttc taataaacct
    30421 gctttttgtt tttctagtaa ttcaaagaga tcgaaatgtt cttccgtttc caatccccaa
    30481 agttcatcta acgcaatatc gaatcggcta cattgcacac gtgtataacc agcttccaac
    30541 tttccttgat ttttccaaat ggaatatagc catgttcgcc aatctgtttg ttgttcacgc
    30601 atgacttcct catattcccg gcaaccttgt cctgaaaatt gcacaagtac attttctgtt
    30661 acagttcgtt ttgaatgcca gaacacccag atattcccac gaacagaaag acagtcaaaa
    30721 agatacaagc ccgttggacg tgtttcaaat tctgtcagat ccattcgtaa tacacgttga
    30781 atcactgttt tgtagtctgt cccaaaaaag gaaagacgca aataatcaat cattgctcga
    30841 agatctattc cgcttttcat atacaatcga aagcgttcct tgattcgttg ttcaaaatcc
    30901 tctgggattt ttcttttccc ttgttccatc tttctaatgt aatcttttga gcagcccatc
    30961 aattgcgcaa atcttgcttg tgatagcggc tttttatctc cacttatatt atgaattgca
    31021 cttaacttct ttctgtagct ttttagctca atcggtgtca ttttctcgct ccttttataa
    31081 aataacaaaa tcgatttttt ttaacccttt agaaacaccg atatatcaac tagtccccct
    31141 cgttttttgg gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt
    31201 ttataaaact tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg
    31261 ctttacacaa agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta
    31321 gcgagtgcgc attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc
    31381 ggctaccaga ctacgtctag agccctcccg atagataata actacaaaat taaatgatct
    31441 aattttgtta tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa
    31501 tcctcttcta aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc
    31561 gttagcaata cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg
    31621 taatcagtta cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa
    31681 ggattaatca ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt
    31741 cacctcctta gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt
    31801 tgctaaatac atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt
    31861 ttgatcttga ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc
    31921 aaattctttg ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac
    31981 atcaaatcct ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt
    32041 tttaaaatct tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat
    32101 acggaatgtt aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat
    32161 tgctaaaata atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt
    32221 atcgactcta tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa
    32281 aaagataaaa tgcggtggta acccataata agcataattt ttccctggtt catatttagg
    32341 atctttcatg gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa
    32401 acaaataatc atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc
    32461 acctaatgct gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa
    32521 acaagcttga attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat
    32581 caacatattt ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc
    32641 attttctgct atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt
    32701 gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa
    32761 atgacccatt tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac
    32821 taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg
    32881 tttctcacta acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag
    32941 taactgacgc caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa
    33001 ccctaaaatt aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag
    33061 atacatttgc caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca
    33121 acctaacaga atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg
    33181 attgaaatag cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat
    33241 tcctcctttc tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac
    33301 aggaatatca catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg
    33361 gtgtgcacac atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg
    33421 tttccactgg tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat
    33481 aaccgcacaa atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc
    33541 aatcaattgt tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta
    33601 tctgaattca aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc
    33661 ctattctttc aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt
    33721 tttgatctaa acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta
    33781 ctttctctaa tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg
    33841 ctggaatata taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa
    33901 aaccagtcaa atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg
    33961 gatttccaaa aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt
    34021 cattatcaaa tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag
    34081 tttcatctaa tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa
    34141 acctccccaa tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga
    34201 cgctctgttt gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca
    34261 aaataagctt cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc
    34321 tcttcagctg tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt
    34381 aattgaacat atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa
    34441 cccaataaaa atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata
    34501 aagtttttcc tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa
    34561 caagcggtta aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt
    34621 tataaagtgt tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac
    34681 tacttgtttg ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt
    34741 gttctggttt cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt
    34801 tgtttgttag atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg
    34861 ttttcgcttg gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca
    34921 ctaagaattc aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat
    34981 gagtgccttc aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga
    35041 tttgaccaat cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg
    35101 ccaagatctc ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga
    35161 ttttgatggt ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc
    35221 taaacagatc ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa
    35281 agtctagatt gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt
    35341 cttttccatc tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg
    35401 tgtatgtttt tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg
    35461 ttaatggatt aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg
    35521 ggttagttac tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt
    35581 catcatttct aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat
    35641 caaaggtaaa tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg
    35701 tttctaccgt atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc
    35761 ctttgaccaa gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg
    35821 taaacaatcc tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa
    35881 cggttggatt attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa
    35941 cttcaatgcc atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac
    36001 tagcattaac cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca
    36061 aggtacttgt gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca
    36121 atactcctga aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt
    36181 taatgacgat cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt
    36241 gtgtttctag ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat
    36301 catctggcgc attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga
    36361 aactttctaa tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta
    36421 aaatttcccc gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca
    36481 aggtaaagtt cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata
    36541 attttacact tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat
    36601 cattggtaat cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga
    36661 tttcttccaa acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc
    36721 ctttttcatt ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga
    36781 cataacgatt ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt
    36841 ttcctgtttc ttgatccact ttcaccactt taagacgttg ttcaattact ttatcttcta
    36901 aaacataatg gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt
    36961 tatacccttc ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt
    37021 taccaaaagt agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg
    37081 tttttgacgt caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg
    37141 aatcgactaa atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat
    37201 cttttgcggt cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag
    37261 aaagcacata accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat
    37321 ctttaatttc tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt
    37381 tcccatctgc ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt
    37441 tcacaccagt ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta
    37501 aattaataac agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg
    37561 gacgacgata ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt
    37621 tatccggtgc ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga
    37681 caccttttgg tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt
    37741 tcaacgtttc gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa
    37801 agttattaat caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt
    37861 tgattgggat ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt
    37921 catcggattt gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc
    37981 gtacatttga atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg
    38041 cggctgagac accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca
    38101 tataatttaa agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc
    38161 aatcaatttt ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac
    38221 gattggttgg tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt
    38281 ttttcttttg attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt
    38341 gactagctgc ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt
    38401 cagcagtagc cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt
    38461 tctcagaaga ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag
    38521 tggacgtttc tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac
    38581 caggactatt ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat
    38641 tgaccgttgg cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc
    38701 cggcttctgc tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg
    38761 taggttgtat agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga
    38821 attgagaaaa agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca
    38881 caagaattcc tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag
    38941 tgataacgac gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc
    39001 atcaatgatt ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa
    39061 atcaactgtc catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa
    39121 tctcatgcca taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc
    39181 ttcatctaat gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag
    39241 atcttctgga agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc
    39301 attactagct actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat
    39361 tcttctttta cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta
    39421 aaaccatcta ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa
    39481 taggtaggtt tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca
    39541 aaataactat ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt
    39601 attgatactt gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat
    39661 aaatattccc gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc
    39721 cccattcgta taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc
    39781 gtcccaacca atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa
    39841 tgccataagt tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac
    39901 acgtgtgcct tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg
    39961 cattttttgc tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct
    40021 tctttataaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40081 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40141 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40201 tttactagaa cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa
    40261 aaacgataga tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc
    40321 attgcttatc aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga
    40381 ccgtacgaat ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa
    40441 taaccctatc ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact
    40501 atgaccatta ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga
    40561 tctccgaatc tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat
    40621 gtggctctgt acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc
    40681 aagcgaactt tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca
    40741 ttttatctag ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc
    40801 cattgagctt gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa
    40861 ccctttttgt ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa
    40921 aacgtcgata acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat
    40981 tctaaagaaa gatttcctat ataagcatat tggataattc gattagtatt gggtatatct
    41041 ctaccattct catacctatc tatggaccat tttgaaattg aaggagaaaa aagctttcca
    41101 aactcctccc tttctagata ataatctttc ctaattttcc tcatccttaa tcccatacta
    41161 aaagaattta tctttttaaa atcgctaact ctttcatctt ttggaagtat attcttataa
    41221 tcttctccgt acataaggta aggaacagtg accttaccaa gataggcaat attcattagt
    41281 ttttcaatat ctggaatatt aatacctttt tcccatctac ttataacatt tttgcctgta
    41341 tagatggtaa agtttttacc aaatttttca agcgtctctt cccggttctt tcggacttct
    41401 cgtattcttt ttcccataac aattttatcg tcttttgata aactaaatat ctcactatca
    41461 cgattaatct ccttcaaaat ttctttttca ttacttaata gaaaaatatc gatttttttt
    41521 tgcatttctt ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta
    41581 attcctatat tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt
    41641 agtttaccga tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc
    41701 agaatcttgc ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat
    41761 ttaactaaag tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt
    41821 ttctcttttc ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca
    41881 cctcctatga gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc
    41941 aattttgtta attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca
    42001 gataatcctc agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt
    42061 ataagttatt tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc
    42121 ggaacttcga cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag
    42181 agtttacgtt tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag
    42241 gctttgatgt ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca
    42301 aattgttggc gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag
    42361 gcaaagtctc ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg
    42421 tccatcttca ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag
    42481 cctttcgtgc ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata
    42541 ttatagacga catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt
    42601 tttcgttgag aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct
    42661 ttgacgaatg gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta
    42721 tctactgtag cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc
    42781 gtgatcaatt ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga
    42841 agtccgagga atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat
    42901 tgtaaagcag catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga
    42961 gaagtatcaa ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt
    43021 aaagcttcag cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg
    43081 acagttttga tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca
    43141 actgcttttg gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc
    43201 agtttgattt tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca
    43261 ccgcggtaac tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct
    43321 ccacttggtg caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct
    43381 gatttggcac tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct
    43441 tgacggacac gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc
    43501 tccaaatcat tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt
    43561 gctgcatcga ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag
    43621 gtgaagtcga cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc
    43681 gctgtgtaat tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa
    43741 tccatcgagt ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct
    43801 aaaatacgat cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta
    43861 cgaatctgct cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata
    43921 taatcagcac tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc
    43981 agctatagaa gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga
    44041 tgaaaaaatg gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga
    44101 acatatcgat caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat
    44161 cgaggaaagc gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga
    44221 agtatcgaaa gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta
    44281 tccttgtgaa gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg
    44341 aacttatcca gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga
    44401 taatatgcgc aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta
    44461 tcggcgtaaa ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca
    44521 gctacctaaa caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga
    44581 cgtattttcc gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga
    44641 ttttcattgg gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta
    44701 ctacgatagc aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt
    44761 tcacgtaaaa gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat
    44821 ttccgcacat ttttccatga tctactgggt acatggagat atttataaaa atgagccttt
    44881 agcatttttg attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg
    44941 gatcgttgat gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt
    45001 tcatttcatc attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg
    45061 ggatgaacag ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga
    45121 tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat
    45181 cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat
    45241 ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt
    45301 ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa
    45361 tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg
    45421 acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg
    45481 ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg
    45541 aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac
    45601 agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat
    45661 ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt
    45721 cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt
    45781 aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat
    45841 aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg
    45901 gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa
    45961 cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt
    46021 ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg
    46081 gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat
    46141 gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga
    46201 ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat
    46261 cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat
    46321 acgatgggcg acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg
    46381 tctcaaagta aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat
    46441 acctaataaa agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc
    46501 gaagaaacta cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt
    46561 cagacccctt ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc
    46621 tgttttgaaa tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac
    46681 atgtcctgca acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt
    46741 caatggtctt gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc
    46801 atcactgatt gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat
    46861 tttgtcaacg attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc
    46921 gattcggttc aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc
    46981 cactgcttct ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat
    47041 attgctggtc atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt
    47101 caagcgtccg tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc
    47161 gatttcatcc aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc
    47221 accttcttcg taacctacat agcctggagg agctccgact agacgagaca cagcatgttt
    47281 ttccatatat tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc
    47341 agctaacgct ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga
    47401 acctagagga cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac
    47461 cgcatcaact gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat
    47521 tagtttttct cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac
    47581 gacttgagca atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt
    47641 tgctttagct tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa
    47701 tacagcagcg cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt
    47761 atcgatttca gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg
    47821 catcttcatc gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa
    47881 gcgtttttta cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg
    47941 catcaaacgt ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat
    48001 cgttgcactg gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat
    48061 ataacggtca gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg
    48121 gtggatctca aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg
    48181 ttctttgact aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg
    48241 acggtactca tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg
    48301 tttcaacaga tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg
    48361 gatttcatcg atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc
    48421 ttttagtctt tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc
    48481 taatgaaaaa atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg
    48541 agcaagccct tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt
    48601 attttttgtt tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat
    48661 gattggatcc atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa
    48721 tgctttgtat tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc
    48781 gatgttttta cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg
    48841 ataatttttt aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc
    48901 gaagctttca cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt
    48961 ttgtccatat tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat
    49021 ttcatgttca aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg
    49081 attagattgt aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt
    49141 gaccgcaatc tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat
    49201 attcatacag gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca
    49261 aaaactatta tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt
    49321 ttttctcgac ttatataaat cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga
    49381 gccaaacaaa cgtaaaatta tcttatccat aacgtttttc tttgatttga taaaaagggt
    49441 agatcttata aaagacctac ccttgatttt ctttattctc tttttttact tctattattt
    49501 cttctttgat cactcttcgc cgttcagcaa tcacatacac aaatccatag agaaatggca
    49561 agataaagga actgaaacga tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa
    49621 ctggtctgaa taataacaaa tagacaaatt caaaggaacc aataccagca ggcgtaggaa
    49681 taacccctga aagcactact gcaaagctga taaacgaaat ggtcagtaga aaatctattt
    49741 taggatggtt ttcaatcaga atcaaataag gaagtacata ccaaaataca agttttaaca
    49801 tattccatag ataaatccgc aatagcgcag tcctgtcttg gataatggtt tggaccgttt
    49861 cccttaaaga gtagatctgc tgattgcatg tatccaccca tttccgcaaa cgctcacttt
    49921 taaataactt attcgataag ctgacaaaaa atatttgaac attcaagctg acggacatcg
    49981 ccaataaaaa ggcaacaatc acgatcgtca aaaggatacc agcgatgata aaccagatca
    50041 ttgatggacc attagaataa aaaagagaga attggatgac caagccagct aatgcataag
    50101 tgatcactgc cagtttgtac ataatcatat ggagtgctgt cacccctgct ccctgggata
    50161 gcttcattcc ttttttgtta taaaaataaa tctccgagat cagcgttcct gtaccaaacg
    50221 aaacgattcg gtaaaacgca atatagctag aggtcaagaa cccatctttc gttgtaaaat
    50281 tcggcgcgaa aggttcagca atttctttga ttgagcgccc ttcagcgatt tggtaaactg
    50341 tccctaatat aacagccgct gccaaaacaa caacactggt agacagcaat tcggcaaaga
    50401 tatctgcaaa tgattgattg attaaataat aaataatggc aaaaatcaca atcagcaaaa
    50461 gcagattcag aatgatttta gttttcgttc ctttagacga catctcctgc tccttaatgg
    50521 aaaaaataaa tgagaatcaa atcaacaagt acacctgcag cgaatcctat agtaaacttc
    50581 tttttccgag ttttatgatg gaaaactttt cgagagatca atcctcccaa accaccgcct
    50641 aacaggctga caaatatcaa attattctca ggaacacgcc agccgctagt tctagcctga
    50701 tgtttatcat accccatcag acaaaataag taaaaattca caatgaaaag gtaaaaccac
    50761 aaaggattgt gtaacaatac atccattact cttcttccaa ctttctgtta actactattt
    50821 ctaagctttt tcttctttct ttggtgaata aactgttaat ccttttgata caacagataa
    50881 ttcaactgga agatcatctg tcgtgtctcc atccgttcga gtgcccacag atttttcctg
    50941 cgcttggatt ttcatgcgtt tggctgtgaa ataagtaatg ccggggatcg tatagaattt
    51001 gcctctcatg attctaggca aataaaaaat aaatcggaaa atatctaagg aaggtacgat
    51061 cgttacatga aattttccat catctagggt cgctgagtcg tcaaaatttg taaaaccgcc
    51121 cacggagtta gacatcgtga ctgtcaacag ttgggatttt cctttccaat gctcctcgtc
    51181 tgtttctaaa tccaaatgat attttttctt cttcagcaat aacttaaaaa acttcttgat
    51241 aaagatccac gagccgtaac gctgtttttc ttcttgactg atccaaactg ctgtatctgc
    51301 taagattccg attgtcagtg tagagataat gacttcatca ttgacttgac catagtctat
    51361 tttccgtaca ttgccttcca ggatcacgtc agcagcttct tcttttttga ggggaatctc
    51421 aagaacacgt gcaaggttgt tgacagttcc acctggaagt aatccgacat ggtattgaga
    51481 taatgttttt tggaacgctt ggacgatatg atgaatcgtg ccgtctccac ctataattac
    51541 taaagtatca gcttctatgg cttctgcttt cttgactaat tccgcatcat caatcgatgg
    51601 gccagtttca gctagttcta catataattc tggctgcttt tcttttacat aatcttgaaa
    51661 ccagcgtgct aatttctcac cctcatcttt ccctgaactt ttgttataaa aaactagcag
    51721 cttttccatt ctttacctcc tatttactct tcaaaaaaga gggcgggaca accaattcca
    51781 ttgtcctgcc ctttagcctt agacagatga cattccatca ttgggacgaa cgccttgtca
    51841 taagctgttc tttttcgatt ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg
    51901 tctgttgctt tttccattac ttgtaaagaa acaaattcga atcgtccgtg catattttct
    51961 cctccggcaa atagatttgg cgtcggaata cccatgtaag agatttttga accatctgtt
    52021 cctccacgaa ctggttcgat cactggagag ataccaagct cgatcattgc ttgtttcgct
    52081 aattccacga tgctcatatc tttttcaatg atttctttca tgttgtaata ttgatcatac
    52141 atatctactt tgattcgttc ttgatcaaat cgctgattca actcttcttg gatatgtgtg
    52201 atttgtgctt tgcgtgcttc gaatttttca cgatcatgat cgcgaatgat ataactcata
    52261 gaagcttctt caggattccc atccaatgcc atcaaatgga agaaaccttc gtatccttcc
    52321 gttttttctg gaacttcatc ggctggaagc tgattatgaa aatcgattgc taactgtaga
    52381 gcattgatca ttgtatcttt tgccgtccct ggatgtacat ttttcccttg gatcgtaata
    52441 tttgcttgag cagcactaaa cgtttcatac tgcaactcac ctaaaggacc accatcgact
    52501 gtataagcaa aatctacatc gaattgctca acatcgaatt tatccgctcc gacaccgatt
    52561 tcttcatcag gaccaaaagc cactttgatg gtaccgtgag ggatagaagg attattgatc
    52621 aagatctcca ttgcagtcat gatttccgca atccctgatt tgtcatctgc tcccagtaat
    52681 gtactgccat ctgttgtgat caatgtgtgg ccttcataat ttttcaaatt tggaaaatct
    52741 tttgtattca atgtatagcg ttcttccgca tctagtacga tcgtagactc gccatcatag
    52801 ttttcaatga tttgtggatt gactcctact gcattaaaat ctgctgtatc catgtgggaa
    52861 ataaatccaa tggaacgcac ttcatgatca acattgcttg gtaatgttcc cataacgaat
    52921 ccgttatctt cattatagaa aacatcagct aagcctaact cttctaattc ttttttcaat
    52981 tcttgcgcaa aagcaacttg tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag
    53041 cgtgtttctg ttttgacata tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc
    53101 tctcctttta attaaattga aatgggtttg tatttgtttc agaaacgaaa aagttaatat
    53161 cccaattttc ttcttgtttc caagattcaa atttctcgat aaatcgttgc ttgcataacg
    53221 attcaatata atgaccggga tcaattgcaa tcaacccagc gctttgcata tcatgagccg
    53281 tatgataata gatatctccc gtgatataga catctgctct ttgggcgatt gcttgcggat
    53341 aaaatttttc accgcttcca ccgcaaatcg caatgcgctt cacagaggat tttgcatttt
    53401 ttggctggac tattctcagt ccatctagtt ggaacgcttc ttttacttgt tctacaaatg
    53461 cctctataga aatttcttgc ggcaattcac ctactcgtcc taacccaaac atttcgactg
    53521 gttcatctat agcgaaaaga tcataagcag gttcctcata aggatgagct gaccgcatgg
    53581 cttgaatcac ttgtttttcg atagtttccg gcaaaataac ctcaactttt gcctcttgaa
    53641 cttgttctgt cttccctact ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc
    53701 gaccgtgtcc aatcgaggta aaacttgtac cggtataatc accttgtgta ccagcaccag
    53761 ctgcagctaa tacttcacgc attttctgtg catgatccac aggtacataa acggccagtt
    53821 ttttataatg aatctcatga gtctttacta aataactctc cacttcaata ccgagaagtt
    53881 cacaaaacca gtcattcagt ccatcccaaa taatatccat gttcgtgtgt gctgcataga
    53941 cggcaatatc atgtttcaac aaatctgcat acattttttc ttgtggctga tcagtaacca
    54001 gccgttttac aggacgaaag attggaggat gtttagcaat cagcaaatcg atttttttct
    54061 tgattgcttc ttcgaccact tctggacgaa cgtctaatgt catcattact cgttggattg
    54121 gcttatccaa tgttccgata tgcagtccga ctgggtctcc ttcttcagct agccactgag
    54181 gacaatagct ttcaaacttt tggatgaaag tgcgtccgct gattgtcatg gctttaaaac
    54241 ctcctcaatc atttggattt cctgctcgat ttgtttttga cgtgcctctt gattagctgc
    54301 ttgtttgagc tgttcacgga tagcttttct ttgttgaagc tctcttgtcc attttgattt
    54361 gaagatctca cttttctctt caagcagaaa tggcccgaac atcagctcca tttcattgta
    54421 agtaactggt ttttgttctt tttcggctac aatgatttca tatatttttt tattttcttc
    54481 aagtattttt tctgattgga ttttatactg attttcttgc agccaagatc gtaatgtacg
    54541 ctctccgatg tttggctgaa ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt
    54601 gccagcttct aggatatctc ggatcagtga acctcccata cccgcaatcg tgacagctgt
    54661 aataccatct tgtggctgga tagcatctag accatttgca aggcgtacgg tgatacggtc
    54721 ttctaatcca tcttttttga cttgcttacg tgctgattca taaggacctt gaacgacttc
    54781 accagcaact gcatattcga tttttcctct taatatcaat gcaacgggta agtaagcgtg
    54841 atctgatcca atgtctgcta aacgagcgga ttcagggaca aagtcaccca ctgcggctaa
    54901 gcgctgagat aaatctaaat ggttcatagc ttcactcctt ctagataatt atagcatttt
    54961 ccaaataaaa taaggagact aggaataaaa aa
//
LOCUS       DACIWG010000016        53768 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.016, whole genome shotgun sequence.
ACCESSION   DACIWG010000016 DACIWG010000000
VERSION     DACIWG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.016"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            7..729
                     /locus_tag="IYF54_000932"
     CDS             7..729
                     /locus_tag="IYF54_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ9831278.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            787..2826
                     /locus_tag="IYF54_000933"
     CDS             787..2826
                     /locus_tag="IYF54_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ9831279.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2935..4086)
                     /locus_tag="IYF54_000934"
     CDS             complement(2935..4086)
                     /locus_tag="IYF54_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9831280.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4240..4770)
                     /locus_tag="IYF54_000935"
     CDS             complement(4240..4770)
                     /locus_tag="IYF54_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831281.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4869..5219)
                     /locus_tag="IYF54_000936"
     CDS             complement(4869..5219)
                     /locus_tag="IYF54_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ9831282.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IYF54_000937"
     CDS             complement(5376..6905)
                     /gene="ahpF"
                     /locus_tag="IYF54_000937"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ9831283.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IYF54_000938"
     CDS             complement(6915..7478)
                     /gene="ahpC"
                     /locus_tag="IYF54_000938"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ9831284.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7622..8284)
                     /locus_tag="IYF54_000939"
     CDS             complement(7622..8284)
                     /locus_tag="IYF54_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9831285.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8468..10114
                     /locus_tag="IYF54_000940"
     CDS             8468..10114
                     /locus_tag="IYF54_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ9831286.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10105..11187
                     /gene="uxuA"
                     /locus_tag="IYF54_000941"
     CDS             10105..11187
                     /gene="uxuA"
                     /locus_tag="IYF54_000941"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9831287.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11295..12848)
                     /locus_tag="IYF54_000942"
     CDS             complement(11295..12848)
                     /locus_tag="IYF54_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9831288.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12996..14284
                     /locus_tag="IYF54_000943"
                     /pseudo
     CDS             12996..14284
                     /locus_tag="IYF54_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14410..15489)
                     /locus_tag="IYF54_000944"
     CDS             complement(14410..15489)
                     /locus_tag="IYF54_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9831289.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15592..16032)
                     /locus_tag="IYF54_000945"
     CDS             complement(15592..16032)
                     /locus_tag="IYF54_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9831290.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16089..16499)
                     /locus_tag="IYF54_000946"
     CDS             complement(16089..16499)
                     /locus_tag="IYF54_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ9831291.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16679..17113)
                     /locus_tag="IYF54_000947"
     CDS             complement(16679..17113)
                     /locus_tag="IYF54_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9831292.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17213..18061)
                     /locus_tag="IYF54_000948"
     CDS             complement(17213..18061)
                     /locus_tag="IYF54_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9831293.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18450..20216
                     /locus_tag="IYF54_000949"
     CDS             18450..20216
                     /locus_tag="IYF54_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ9831294.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20318..21577
                     /locus_tag="IYF54_000950"
     CDS             20318..21577
                     /locus_tag="IYF54_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9831295.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21724..23025
                     /locus_tag="IYF54_000951"
     CDS             21724..23025
                     /locus_tag="IYF54_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831296.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23028..23879
                     /locus_tag="IYF54_000952"
     CDS             23028..23879
                     /locus_tag="IYF54_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831297.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23892..24707
                     /locus_tag="IYF54_000953"
     CDS             23892..24707
                     /locus_tag="IYF54_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ9831298.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24858..26024
                     /locus_tag="IYF54_000954"
     CDS             24858..26024
                     /locus_tag="IYF54_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9831299.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26254..26901
                     /locus_tag="IYF54_000955"
     CDS             26254..26901
                     /locus_tag="IYF54_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ9831300.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSETSTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26985..27929
                     /locus_tag="IYF54_000956"
     CDS             26985..27929
                     /locus_tag="IYF54_000956"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ9831301.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28064..29515
                     /locus_tag="IYF54_000957"
     CDS             28064..29515
                     /locus_tag="IYF54_000957"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ9831302.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29536..30372
                     /locus_tag="IYF54_000958"
     CDS             29536..30372
                     /locus_tag="IYF54_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ9831303.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30372..31031
                     /locus_tag="IYF54_000959"
     CDS             30372..31031
                     /locus_tag="IYF54_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9831304.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31157..31846
                     /locus_tag="IYF54_000960"
     CDS             31157..31846
                     /locus_tag="IYF54_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831305.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31852..32808
                     /locus_tag="IYF54_000961"
     CDS             31852..32808
                     /locus_tag="IYF54_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ9831306.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32852..33364
                     /locus_tag="IYF54_000962"
     CDS             32852..33364
                     /locus_tag="IYF54_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831307.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33620..34168)
                     /locus_tag="IYF54_000963"
     CDS             complement(33620..34168)
                     /locus_tag="IYF54_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9831308.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34257..35438)
                     /locus_tag="IYF54_000964"
     CDS             complement(34257..35438)
                     /locus_tag="IYF54_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ9831309.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35674..37182)
                     /locus_tag="IYF54_000965"
     CDS             complement(35674..37182)
                     /locus_tag="IYF54_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ9831310.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37341..37532
                     /locus_tag="IYF54_000966"
     CDS             37341..37532
                     /locus_tag="IYF54_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831311.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37735..38652
                     /locus_tag="IYF54_000967"
     CDS             37735..38652
                     /locus_tag="IYF54_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ9831312.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38645..39718
                     /locus_tag="IYF54_000968"
     CDS             38645..39718
                     /locus_tag="IYF54_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831313.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39866..40384
                     /locus_tag="IYF54_000969"
     CDS             39866..40384
                     /locus_tag="IYF54_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831314.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40365..40757
                     /locus_tag="IYF54_000970"
     CDS             40365..40757
                     /locus_tag="IYF54_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ9831315.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40833..41768
                     /locus_tag="IYF54_000971"
     CDS             40833..41768
                     /locus_tag="IYF54_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ9831316.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41901..42680
                     /locus_tag="IYF54_000972"
     CDS             41901..42680
                     /locus_tag="IYF54_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ9831317.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42673..42990
                     /locus_tag="IYF54_000973"
     CDS             42673..42990
                     /locus_tag="IYF54_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9831318.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43234..44844
                     /locus_tag="IYF54_000974"
     CDS             43234..44844
                     /locus_tag="IYF54_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9831319.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45001..45849
                     /locus_tag="IYF54_000975"
     CDS             45001..45849
                     /locus_tag="IYF54_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9831320.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46080..46559
                     /locus_tag="IYF54_000976"
     CDS             46080..46559
                     /locus_tag="IYF54_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831321.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46592..48820
                     /locus_tag="IYF54_000977"
     CDS             46592..48820
                     /locus_tag="IYF54_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ9831322.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            48949..50592
                     /locus_tag="IYF54_000978"
     CDS             48949..50592
                     /locus_tag="IYF54_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ9831323.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50665..51210)
                     /locus_tag="IYF54_000979"
     CDS             complement(50665..51210)
                     /locus_tag="IYF54_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ9831324.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51233..51718)
                     /locus_tag="IYF54_000980"
     CDS             complement(51233..51718)
                     /locus_tag="IYF54_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9831325.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51708..52520)
                     /locus_tag="IYF54_000981"
     CDS             complement(51708..52520)
                     /locus_tag="IYF54_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ9831326.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52659..>53768)
                     /locus_tag="IYF54_000982"
     CDS             complement(52659..>53768)
                     /locus_tag="IYF54_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ9831327.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17904 a         9783 c        10380 g        15701 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt
     1861 ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg
     1921 cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat
     1981 tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat
     2041 gctcctggat ctacgtttaa aataattaca gcagcaatcg gtctcgacaa cggcactatc
     2101 gatccaaatg aagtgttgac gatcaacggg cttaaatggc aaaaagacag ttcttgggga
     2161 tcttttcaaa caacgcgtgt cagtagtgat gtatcacaag tagacttaaa aactgctttg
     2221 atctattccg ataatatata tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt
     2281 cgtacaggct tagataaatt catttttggt gaagaccttg atttgccaat tagtatgaat
     2341 ccagcacaaa tttctaatga agatagcttt aactcagata tcttgctagc tgatactgga
     2401 tatggacagg gcgaacttct aattaatcct atccagcaag cagcaatgta ttctgttttt
     2461 accaacaatg gcacacttgt ctatcctaaa ttgattgcag ataaagagac aaaagataag
     2521 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg
     2581 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2641 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2701 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     2761 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     2821 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     2881 ttttcaaata aacggttttc acgatgtctc tttcacaact tctttttgat ttactcacac
     2941 ttcataaact ttttttgtaa aatatttgag aacaagtgga gaaataagtg tacttaataa
     3001 aatcacgata actaaaggtg aatagtactg tggttcgatc aattgacttt gctgtccaat
     3061 ttgtaaaatg atcaaagcca tttctccacg cgaaatcata ccagcaccga ccatcaatga
     3121 actatgttga gaaaaaccgg caatctgagc accaaaataa cctccaagca atttggtcaa
     3181 aatcgctaca agcgtcaata tagaaataaa gagtagttgt tcactaaagt cagaaaaatc
     3241 aacttctaat cctacactga caaaaaatac tggtataaat actgcatatc ctaaagcctc
     3301 tacattgtgg tatacttctt ctttaacctt tgtctgactg acagctatcc cagcaaaaaa
     3361 agcaccaatg acagagctaa gcccaacaag atccgctacg taagacattc ctaaacaaat
     3421 aactagcgat acgatgataa tagaagaatt tgcgtacact ttttctgcaa atgaaagtag
     3481 gaaaggcgca atccatttga ctaataagaa aataaaaaca aaataaatta gctgttcaat
     3541 caaaacaagc ggtaaagaag cattgctaga agcttccccc gttagaaaag ataggctgaa
     3601 acttagaact aaaacgacta aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc
     3661 ttcttttgta ttcactacgt ttagttcttt taatacttct actgaaatac tgacagaagt
     3721 tgccgcaaga ataatcccaa agaaaatcgc ttcatttccc gaaacttgga atgctttgcc
     3781 tgtcaaccag ccaaaaacaa ccgggaagat gatcccaagc aaagcaacga acataccagg
     3841 tcggaaatat tttttcaaaa gagataaatc actttccaga ccagctaaaa acattaataa
     3901 gatcacacca atctccgaaa agtcatggac tagaatatca ggtttgaccc aattcaatcc
     3961 agcttggcct agcaaaacac caacaagtaa ttgtccaatc actgctggga tacctaatct
     4021 ccgcgagata tgtgagccaa tcgttgttga aatcaatatc aggcataaaa taccaataaa
     4081 ttccattctc atcattcctt tgtataaaat atagcccgaa cttagcataa catacattat
     4141 tgaaaaaata ggaaaatata ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt
     4201 gttggttttt atacggtcta ttttaataac taaaaatgat taacgtatag tcatttttct
     4261 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
     4321 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
     4381 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
     4441 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
     4501 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
     4561 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
     4621 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
     4681 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
     4741 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
     4801 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
     4861 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
     4921 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
     4981 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
     5041 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
     5101 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
     5161 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
     5221 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     5281 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     5341 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     5401 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     5461 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     5521 actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt
     5581 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     5641 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     5701 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     5761 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     5821 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     5881 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     5941 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     6001 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     6061 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     6121 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     6181 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     6241 gcactactag ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac
     6301 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     6361 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     6421 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     6481 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     6541 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     6601 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     6661 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     6721 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     6781 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     6841 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     6901 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     6961 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     7021 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     7081 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     7141 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     7201 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     7261 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     7321 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     7381 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     7441 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     7501 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     7561 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     7621 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     7681 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     7741 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     7801 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     7861 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     7921 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     7981 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     8041 ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt
     8101 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     8161 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta
     8221 agtcagcata gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt
     8281 tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa
     8341 acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca
     8401 tagcgatttt tattgtataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag
     8461 gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga
     8521 agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt
     8581 ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg
     8641 tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga
     8701 aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag
     8761 cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc
     8821 agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt
     8881 agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat
     8941 aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat
     9001 tcttgctgcg ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc
     9061 tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa
     9121 agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga
     9181 aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt
     9241 tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg
     9301 aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca
     9361 ttttcccaat ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac
     9421 ggtagataaa gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt
     9481 agctattatg ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga
     9541 tttatcgctg ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc
     9601 aggtatcatt gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc
     9661 aaatattcct gatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg
     9721 ttatggtgag acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt
     9781 attcatacca ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg
     9841 aaaaccattc atcccgagtc cagacccact attgacagac tttcagcata agctttccga
     9901 tattcaactg ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc
     9961 aaataaagaa atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga
    10021 aatgttcatc aagatggtag caggaccgaa tgccgtacga aaaacattac aggattactt
    10081 acaagaacat ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa
    10141 gaaaagatta ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca
    10201 ttgatggata taccagtagg agaggtatgg cccagggaga ggatcaaaga actaaaagaa
    10261 gaaattgagt ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc
    10321 attaaaattg gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga
    10381 aacttggcag aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg
    10441 gtgaaatctg accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct
    10501 gatgatattc cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa
    10561 tttgaattgc cgggatggga acctgagcgc ttagctcata tcaaatcctt gcttgaagcc
    10621 tatgcatcag tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc
    10681 atcccaacat gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac
    10741 tcgatttttg gattacctcg aatcgtaaag aatcgagaag atttagattg gttgtgccgc
    10801 atagtagaca gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca
    10861 gaaaatgatg tatatgggat tttagcagaa ttttgtcaaa gagatcgaat cccatttgca
    10921 catgttcgaa atattaaatt tttagctaat cgagatttct atgaggcacc gcataaatcc
    10981 gaatacggtt cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac
    11041 gaatacatcc gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac
    11101 ggcttatatg atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa
    11161 aaaggaaaca aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag
    11221 aaggatgttc aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat
    11281 gtatcttatt tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct
    11341 tcttctttta attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct
    11401 ggtgtgatga ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa
    11461 gggcagtcgc tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat
    11521 tcatttgaaa aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc
    11581 gtatagttca caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact
    11641 aggtcttcaa ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga
    11701 ttttgttctc tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaacacg
    11761 ctatgttctt gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga
    11821 aatccattat aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca
    11881 ggtataaaca aatcaaatac agtacaaaag gactccacca tttctcttga ataaagaaca
    11941 tgaggaacgt ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg
    12001 attttcgtga tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt
    12061 ggcagattag gtcgcaaaat atctacagag ggatcaataa aaaagtcaat atattcatac
    12121 tctgtgaatt ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt
    12181 tttaatagaa aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt
    12241 ttaaaaaaag tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta
    12301 gcagtacttg aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct
    12361 tttccagaaa aagactttct gccattatta gaaaaggaga tagacaaacc gtacaatgct
    12421 aaaaatgcat taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc
    12481 tgacttaaac tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa
    12541 ctgactgatt cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt
    12601 ccttcaaaat tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg
    12661 agacgctcaa agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata
    12721 tggaaatgat ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga
    12781 aaaatatcta attttgtatt gctttttttc ttcaagagaa acgttttaat cgcttcttga
    12841 gtttgcatga aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt
    12901 ttttcataaa tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt
    12961 tcttgcatac aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat
    13021 caacatttgc agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga
    13081 tagaaacagc caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg
    13141 atgaattttc atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca
    13201 tcaaagcaaa aattgactat tcaggagact tagcacctat actacaatca gctgttacat
    13261 ttgagaatgt tgatcccgct ttgtcgattg gctatgacga gaacactttg actatccaac
    13321 acaagcaagc gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc
    13381 tttttactgt caaggtatca gatggactac cgaacgatca tcatcattta acgccgtata
    13441 gtcagcggca gacaattgag caagattcag ctatcgactc aggaggagat cttgttgaag
    13501 aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag
    13561 acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag
    13621 agccagacaa tgaatcaaaa cctgaaataa cccctggaga aaaaccagat gtagacccag
    13681 aagaaaaacc agatgtgact ccagaaccag atacggattc tggaaaccaa acagtaccgg
    13741 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagccccag
    13801 aagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
    13861 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
    13921 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
    13981 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
    14041 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
    14101 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
    14161 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
    14221 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
    14281 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
    14341 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
    14401 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
    14461 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
    14521 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
    14581 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
    14641 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
    14701 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
    14761 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
    14821 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
    14881 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
    14941 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
    15001 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
    15061 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
    15121 tgcatctttt ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg
    15181 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
    15241 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
    15301 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
    15361 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
    15421 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
    15481 gttattcatt atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat
    15541 gacgttatct tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt
    15601 tttctggtct tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa
    15661 ttcttcctgt tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt
    15721 ttgcaacgca gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc
    15781 acgctgacga ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc
    15841 agaatccaga cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa
    15901 aactaacatc acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta
    15961 gtgtctaatg atttctttag aggcggcata taaaggaaaa catacttgat taactaaata
    16021 tagatcttcc atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac
    16081 aaaaaagtct attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt
    16141 gaatacggac acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga
    16201 ccttcgatag atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa
    16261 agggaaactt gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat
    16321 tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt
    16381 gcattttcga cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta
    16441 tgcacttctc cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata
    16501 aaattaccac ctttaattat attttataca attgaatttt acacaatata attgtggaaa
    16561 gcaaagaaat ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc
    16621 tcgaatagca aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt
    16681 acatatattt ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat
    16741 gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca
    16801 tcatttctgt catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt
    16861 cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag
    16921 cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa
    16981 aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa
    17041 agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg
    17101 ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat
    17161 tataccagct aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga
    17221 ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg
    17281 cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa
    17341 cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa
    17401 ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca
    17461 tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag
    17521 tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa
    17581 gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt
    17641 ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa
    17701 tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag
    17761 taatcattgt ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta
    17821 ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata acgatattca
    17881 acactaaaat aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac
    17941 cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta
    18001 atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca
    18061 tttattcact tccttaattc attaataata ctcttatctt attataactt acttttcacc
    18121 ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt
    18181 ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa
    18241 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt
    18301 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
    18361 tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga
    18421 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
    18481 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
    18541 aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa
    18601 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
    18661 gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg
    18721 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
    18781 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
    18841 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
    18901 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
    18961 aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc
    19021 ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
    19081 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
    19141 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
    19201 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
    19261 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
    19321 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
    19381 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
    19441 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
    19501 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
    19561 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
    19621 ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
    19681 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
    19741 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
    19801 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
    19861 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
    19921 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
    19981 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag
    20041 ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt
    20101 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
    20161 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
    20221 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
    20281 gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
    20341 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
    20401 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
    20461 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
    20521 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
    20581 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
    20641 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
    20701 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
    20761 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
    20821 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
    20881 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
    20941 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
    21001 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
    21061 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
    21121 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
    21181 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
    21241 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
    21301 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
    21361 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
    21421 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
    21481 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
    21541 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
    21601 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
    21661 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
    21721 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
    21781 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
    21841 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
    21901 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
    21961 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
    22021 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
    22081 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
    22141 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
    22201 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
    22261 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
    22321 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
    22381 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
    22441 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
    22501 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
    22561 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
    22621 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
    22681 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
    22741 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
    22801 aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga
    22861 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
    22921 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
    22981 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
    23041 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
    23101 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
    23161 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
    23221 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
    23281 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
    23341 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
    23401 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
    23461 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
    23521 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
    23581 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
    23641 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
    23701 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
    23761 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
    23821 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
    23881 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
    23941 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
    24001 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
    24061 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
    24121 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
    24181 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
    24241 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
    24301 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
    24361 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
    24421 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
    24481 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
    24541 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
    24601 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
    24661 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
    24721 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
    24781 aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
    24841 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
    24901 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
    24961 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
    25021 gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
    25081 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
    25141 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
    25201 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
    25261 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
    25321 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
    25381 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
    25441 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
    25501 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
    25561 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
    25621 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
    25681 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
    25741 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
    25801 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
    25861 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
    25921 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
    25981 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
    26041 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
    26101 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
    26161 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
    26221 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
    26281 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
    26341 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
    26401 agcacatctt cgtctagcca gacaaaaagt tctagtgaaa caagtactca gtcagatatc
    26461 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
    26521 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
    26581 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
    26641 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
    26701 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
    26761 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
    26821 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
    26881 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
    26941 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
    27001 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
    27061 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
    27121 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
    27181 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
    27241 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
    27301 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
    27361 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
    27421 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
    27481 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
    27541 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
    27601 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
    27661 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
    27721 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
    27781 gcgcaagcat gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta
    27841 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
    27901 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
    27961 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
    28021 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
    28081 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
    28141 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
    28201 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
    28261 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    28321 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    28381 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    28441 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    28501 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    28561 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    28621 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    28681 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    28741 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    28801 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    28861 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    28921 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    28981 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    29041 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    29101 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    29161 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    29221 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    29281 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    29341 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    29401 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    29461 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    29521 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    29581 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    29641 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    29701 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    29761 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    29821 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    29881 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    29941 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    30001 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    30061 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    30121 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    30181 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    30241 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    30301 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    30361 gtgagtgagt gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt
    30421 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    30481 atctggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    30541 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    30601 tatattttgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    30661 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
    30721 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
    30781 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
    30841 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
    30901 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
    30961 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc
    31021 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
    31081 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
    31141 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
    31201 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
    31261 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
    31321 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
    31381 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
    31441 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
    31501 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
    31561 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
    31621 aggcgtaaaa tattttccaa cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
    31681 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
    31741 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
    31801 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
    31861 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
    31921 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
    31981 aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa
    32041 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
    32101 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
    32161 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
    32221 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
    32281 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
    32341 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
    32401 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
    32461 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
    32521 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
    32581 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
    32641 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
    32701 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
    32761 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
    32821 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
    32881 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    32941 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    33001 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    33061 aagcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    33121 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    33181 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    33241 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    33301 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    33361 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    33421 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    33481 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    33541 aaactccctt catcgagagg tcgtgacata gcttttgtca cgacctctcg gcttatccta
    33601 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    33661 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    33721 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    33781 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    33841 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    33901 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    33961 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    34021 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    34081 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    34141 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    34201 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtatattat
    34261 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    34321 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    34381 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    34441 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    34501 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    34561 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    34621 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    34681 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    34741 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    34801 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    34861 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    34921 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    34981 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
    35041 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    35101 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    35161 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    35221 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    35281 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    35341 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    35401 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    35461 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    35521 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    35581 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    35641 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    35701 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    35761 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    35821 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    35881 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    35941 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    36001 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    36061 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    36121 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    36181 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    36241 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    36301 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    36361 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    36421 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    36481 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    36541 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    36601 gctttcatga tacagttggt tcaaggaagt agcgagcttt ccacttcctg ggccaggagc
    36661 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    36721 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    36781 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    36841 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    36901 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    36961 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    37021 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc
    37081 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    37141 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    37201 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    37261 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    37321 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    37381 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    37441 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    37501 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    37561 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    37621 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    37681 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    37741 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa tgcggatcag
    37801 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    37861 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    37921 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    37981 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    38041 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    38101 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    38161 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    38221 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    38281 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    38341 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    38401 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    38461 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    38521 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    38581 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    38641 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    38701 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    38761 aatccctatg ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc cgacgaatac
    38821 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    38881 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    38941 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    39001 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    39061 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    39121 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    39181 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    39241 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    39301 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    39361 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    39421 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    39481 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    39541 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    39601 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    39661 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    39721 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    39781 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    39841 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    39901 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    39961 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    40021 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    40081 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    40141 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    40201 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    40261 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    40321 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    40381 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    40441 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    40501 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt
    40561 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    40621 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    40681 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    40741 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    40801 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    40861 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    40921 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    40981 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    41041 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    41101 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    41161 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    41221 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    41281 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    41341 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    41401 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    41461 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    41521 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    41581 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    41641 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    41701 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    41761 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    41821 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    41881 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    41941 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    42001 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    42061 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    42121 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    42181 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    42241 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    42301 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    42361 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    42421 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    42481 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    42541 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    42601 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    42661 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    42721 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    42781 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    42841 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    42901 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    42961 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    43021 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    43081 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    43141 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    43201 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    43261 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    43321 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    43381 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    43441 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    43501 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    43561 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    43621 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    43681 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    43741 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    43801 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    43861 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    43921 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    43981 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    44041 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    44101 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    44161 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    44221 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    44281 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    44341 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    44401 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    44461 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    44521 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    44581 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    44641 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    44701 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    44761 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    44821 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    44881 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    44941 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    45001 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    45061 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    45121 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    45181 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    45241 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    45301 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    45361 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    45421 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt
    45481 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    45541 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    45601 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    45661 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    45721 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    45781 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    45841 gcgcaataat tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact
    45901 ttctacatgt tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg
    45961 aagctctttt tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta
    46021 aatgagtaaa ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa
    46081 tggaaatacc aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag
    46141 ttgatggaag aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt
    46201 ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat
    46261 ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg
    46321 cacaaggaag tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa
    46381 atccagtcgt agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca
    46441 aaaatccaaa gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag
    46501 aactgattgg ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat
    46561 agacagagat gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg
    46621 aaccttttca cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag
    46681 tgcagaagaa tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa
    46741 aatcagtaac gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc
    46801 actatctgct gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa
    46861 atcagatgtt ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga
    46921 attactagaa gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa
    46981 ccattacggc attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat
    47041 cgaagctgat tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc
    47101 gattgaaaaa ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt
    47161 tcttgtggca cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat
    47221 cattcgtcgc catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa
    47281 tcgcccatta aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc
    47341 tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact
    47401 tctcttaaag atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat
    47461 caaaggtgta gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca
    47521 agatttactc cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga
    47581 attggcaatt cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat
    47641 attgagaaaa gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag
    47701 cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga
    47761 tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac
    47821 aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg
    47881 aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat
    47941 ggaagcacat gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa
    48001 ggaaacgatt catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac
    48061 gccggaagca cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca
    48121 aattttagag ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg
    48181 tcagcggttc aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa
    48241 cccgccacat gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat
    48301 gatacgggat gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc
    48361 taagaaagtc gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga
    48421 aaagacttct ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa
    48481 attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc
    48541 agaaaaatta aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca
    48601 gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt
    48661 gaaagaatat gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga
    48721 agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt
    48781 tcccaagcaa atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac
    48841 gcacttagca gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa
    48901 aatagaatcg gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa
    48961 caaaaatggt ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc
    49021 aatggcgatg gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat
    49081 cttggtatcg gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt
    49141 tatgacgtaa gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat
    49201 ttattgctaa aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac
    49261 catagttcgg atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat
    49321 cgcgattatt acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg
    49381 attttcggcg gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt
    49441 tttgcaaaag aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc
    49501 aatatgatcc gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct
    49561 catgtaaaaa aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa
    49621 aatgtctcag gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat
    49681 atcatgactg tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg
    49741 aaagacggtt atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa
    49801 aaagaagggc aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca
    49861 ttggatggta tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt
    49921 tcagtgtttg gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg
    49981 atgtatttct ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg
    50041 aatatgccct ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa
    50101 aggctgcttg cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg
    50161 agagacaaca gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca
    50221 catgaacctt ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa
    50281 tatgaaccag agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat
    50341 aaaggtctga tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc
    50401 tatgaaagat atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt
    50461 gctgactatg aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc
    50521 tctagcggag aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa
    50581 acaaacaagt aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc
    50641 agattgctct gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg
    50701 acgagagcga agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg
    50761 tacttggaca tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg
    50821 tccaactacg tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca
    50881 agcacctcca gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc
    50941 cactgttcgt tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc
    51001 aatttcttta ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc
    51061 actggaattt tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa
    51121 aagatcacct tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg
    51181 gtcttttaat tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt
    51241 gcttccgtgc ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa
    51301 tcagattttg gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa
    51361 tccaaccggc gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc
    51421 cccacatacg agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga
    51481 tcactaatga aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac
    51541 tggaagcacc aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc
    51601 cggcctcgca taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg
    51661 aaagggcaac tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt
    51721 ctcattcctc ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc
    51781 acgccattag aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa
    51841 tctgccccta acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt
    51901 actgttgtag tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga
    51961 agatccacag cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt
    52021 cctgaagcag tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct
    52081 tgaccagaaa gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa
    52141 agttcttcga tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat
    52201 gccaaaacag ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca
    52261 accatgattg cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt
    52321 ttatcaatcg aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat
    52381 cgctcattaa tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa
    52441 ttctcaaaag tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca
    52501 attgtcaaga ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca
    52561 aaaaagaaaa acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga
    52621 aaagaaagac caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat
    52681 tctttctcga tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa
    52741 tcacggatga tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc
    52801 caatccattt ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc
    52861 cttacaatca ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct
    52921 aatgtactat tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca
    52981 tgcttatcca taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg
    53041 aaccgtgcat cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga
    53101 ccatctgtgt tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg
    53161 tcttccagtc caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga
    53221 gccttaagaa tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc
    53281 tgtgccatcg acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg
    53341 gagtgatagt atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac
    53401 tgaacaaagt tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga
    53461 acaacttttg aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa
    53521 gaagagatac caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc
    53581 ttttccgaaa tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga
    53641 gcagaatgac aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa
    53701 atttcatttt tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa
    53761 agaatagg
//
LOCUS       DACIWG010000017        52450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.017, whole genome shotgun sequence.
ACCESSION   DACIWG010000017 DACIWG010000000
VERSION     DACIWG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.017"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            1..794
                     /locus_tag="IYF54_000983"
                     /pseudo
     CDS             1..794
                     /locus_tag="IYF54_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            934..1860
                     /gene="cysK"
                     /locus_tag="IYF54_000984"
     CDS             934..1860
                     /gene="cysK"
                     /locus_tag="IYF54_000984"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ9831328.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2096..2542
                     /locus_tag="IYF54_000985"
     CDS             2096..2542
                     /locus_tag="IYF54_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9831329.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2814..4166
                     /locus_tag="IYF54_000986"
     CDS             2814..4166
                     /locus_tag="IYF54_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ9831330.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4337..4624
                     /locus_tag="IYF54_000987"
     CDS             4337..4624
                     /locus_tag="IYF54_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831331.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4850..5293
                     /locus_tag="IYF54_000988"
     CDS             4850..5293
                     /locus_tag="IYF54_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9831332.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5299..6483)
                     /locus_tag="IYF54_000989"
     CDS             complement(5299..6483)
                     /locus_tag="IYF54_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ9831333.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6751..7449
                     /locus_tag="IYF54_000990"
     CDS             6751..7449
                     /locus_tag="IYF54_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ9831334.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7456..8133
                     /gene="nth"
                     /locus_tag="IYF54_000991"
     CDS             7456..8133
                     /gene="nth"
                     /locus_tag="IYF54_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ9831335.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8259..8792)
                     /locus_tag="IYF54_000992"
     CDS             complement(8259..8792)
                     /locus_tag="IYF54_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831336.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9089..11470)
                     /locus_tag="IYF54_000993"
     CDS             complement(9089..11470)
                     /locus_tag="IYF54_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ9831337.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IYF54_000994"
     CDS             complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IYF54_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ9831338.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12171..12710
                     /locus_tag="IYF54_000995"
     CDS             12171..12710
                     /locus_tag="IYF54_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ9831339.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12800..13219
                     /gene="gpsB"
                     /locus_tag="IYF54_000996"
     CDS             12800..13219
                     /gene="gpsB"
                     /locus_tag="IYF54_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ9831340.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13241..13608
                     /gene="rnpB"
                     /locus_tag="IYF54_000997"
     ncRNA           13241..13608
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IYF54_000997"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13817..14983
                     /locus_tag="IYF54_000998"
     CDS             13817..14983
                     /locus_tag="IYF54_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ9831341.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15029..16525
                     /locus_tag="IYF54_000999"
     CDS             15029..16525
                     /locus_tag="IYF54_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ9831342.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16606..17289
                     /locus_tag="IYF54_001000"
     CDS             16606..17289
                     /locus_tag="IYF54_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9831343.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17934..19124
                     /locus_tag="IYF54_001001"
     CDS             17934..19124
                     /locus_tag="IYF54_001001"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ9831344.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19226..20179
                     /locus_tag="IYF54_001002"
     CDS             19226..20179
                     /locus_tag="IYF54_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ9831345.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20248..21597
                     /locus_tag="IYF54_001003"
     CDS             20248..21597
                     /locus_tag="IYF54_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9831346.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21780..22103)
                     /locus_tag="IYF54_001004"
     CDS             complement(21780..22103)
                     /locus_tag="IYF54_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831347.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22232..23395
                     /locus_tag="IYF54_001005"
     CDS             22232..23395
                     /locus_tag="IYF54_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831348.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23465..24814
                     /locus_tag="IYF54_001006"
     CDS             23465..24814
                     /locus_tag="IYF54_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9831349.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24859..25290)
                     /locus_tag="IYF54_001007"
     CDS             complement(24859..25290)
                     /locus_tag="IYF54_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831350.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25678..28320
                     /gene="alaS"
                     /locus_tag="IYF54_001008"
     CDS             25678..28320
                     /gene="alaS"
                     /locus_tag="IYF54_001008"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ9831351.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28520..29116
                     /locus_tag="IYF54_001009"
     CDS             28520..29116
                     /locus_tag="IYF54_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9831352.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29238..30386
                     /locus_tag="IYF54_001010"
     CDS             29238..30386
                     /locus_tag="IYF54_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ9831353.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30424..30837)
                     /locus_tag="IYF54_001011"
     CDS             complement(30424..30837)
                     /locus_tag="IYF54_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ9831354.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30916..31347
                     /locus_tag="IYF54_001012"
     CDS             30916..31347
                     /locus_tag="IYF54_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ9831355.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31446..31679)
                     /locus_tag="IYF54_001013"
     CDS             complement(31446..31679)
                     /locus_tag="IYF54_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ9831356.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31862..33532
                     /locus_tag="IYF54_001014"
     CDS             31862..33532
                     /locus_tag="IYF54_001014"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ9831357.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33679..34065
                     /locus_tag="IYF54_001015"
     CDS             33679..34065
                     /locus_tag="IYF54_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831358.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34052..34750
                     /locus_tag="IYF54_001016"
     CDS             34052..34750
                     /locus_tag="IYF54_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9831359.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34755..35219
                     /locus_tag="IYF54_001017"
     CDS             34755..35219
                     /locus_tag="IYF54_001017"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ9831360.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35216..36127
                     /locus_tag="IYF54_001018"
     CDS             35216..36127
                     /locus_tag="IYF54_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9831361.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36362..36901
                     /gene="pyrR"
                     /locus_tag="IYF54_001019"
     CDS             36362..36901
                     /gene="pyrR"
                     /locus_tag="IYF54_001019"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ9831362.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36915..38198
                     /locus_tag="IYF54_001020"
     CDS             36915..38198
                     /locus_tag="IYF54_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ9831363.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38211..39137
                     /locus_tag="IYF54_001021"
     CDS             38211..39137
                     /locus_tag="IYF54_001021"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ9831364.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39156..40436
                     /locus_tag="IYF54_001022"
     CDS             39156..40436
                     /locus_tag="IYF54_001022"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ9831365.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40441..41517
                     /gene="carA"
                     /locus_tag="IYF54_001023"
     CDS             40441..41517
                     /gene="carA"
                     /locus_tag="IYF54_001023"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ9831366.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41522..44707
                     /gene="carB"
                     /locus_tag="IYF54_001024"
     CDS             41522..44707
                     /gene="carB"
                     /locus_tag="IYF54_001024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ9831367.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44761..45537
                     /locus_tag="IYF54_001025"
     CDS             44761..45537
                     /locus_tag="IYF54_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ9831368.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45534..46466
                     /locus_tag="IYF54_001026"
     CDS             45534..46466
                     /locus_tag="IYF54_001026"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ9831369.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46463..47179
                     /gene="pyrF"
                     /locus_tag="IYF54_001027"
     CDS             46463..47179
                     /gene="pyrF"
                     /locus_tag="IYF54_001027"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ9831370.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47186..47815
                     /locus_tag="IYF54_001028"
     CDS             47186..47815
                     /locus_tag="IYF54_001028"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ9831371.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47803..48117
                     /locus_tag="IYF54_001029"
     CDS             47803..48117
                     /locus_tag="IYF54_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831372.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48155..48796
                     /locus_tag="IYF54_001030"
     CDS             48155..48796
                     /locus_tag="IYF54_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9831373.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48827..49531
                     /locus_tag="IYF54_001031"
     CDS             48827..49531
                     /locus_tag="IYF54_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ9831374.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49528..50430
                     /locus_tag="IYF54_001032"
     CDS             49528..50430
                     /locus_tag="IYF54_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9831375.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50588..52294)
                     /locus_tag="IYF54_001033"
     CDS             complement(50588..52294)
                     /locus_tag="IYF54_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ9831376.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17147 a         9140 c        11269 g        14894 t
ORIGIN      
        1 ggaacgagtc ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca
       61 gatgaacaag gatctcaaac agttgctgat cctcaagcgt ttatcgatca aatcggctgg
      121 tctgcgtcag aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg
      181 ttgttggaaa gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc
      241 ggtgtgaaag gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaacg
      301 gacaatgggt aacgatgaat gaaccattcc gaaaatattc ttcttattgg gaatccttcc
      361 aagaccatgc gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg
      421 gcgtgtggaa aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt
      481 atgctaccga tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa
      541 cccgttttga tacgccatca acaggttcgg ctaccacaag tacaacaaca acgactacta
      601 cgacgacttc aacgtcatca tcttcaacaa cagatgtaaa tacaaataat ggtcagacat
      661 atacagtcgt gtcaggtgat acgctatggg atattgcaca aaaatttggc atgtctgttg
      721 atgatctaat ggcgaaaaat ggcatgtcta tgtccagtta ttctttatta gttggtcaag
      781 aactaaacgt ttgatttaga taaaaaggca aagaaactac catatatggt atatctttgt
      841 cttttttttg ctagtcaaag acgaatcact cgccataaat gaacagattg tattatgata
      901 agtaagaaga gcaaagacaa ggaggaattg aagatgacag ctatctataa ctcagtcacc
      961 gaattgattg gcaaaacgcc aatcgtcaaa ttaaataaaa tcgtaccaga agattcagca
     1021 gatgtttttg taaagcttga gttttttaat ccaggaggaa gtgtaaaaga tcgtattgct
     1081 ttaagtatga tagaaaaagc agaacacgat ggtctactga aaccaggtga tactatcatt
     1141 gaacctactt ctggaaatac aggaatcgga ttgtcgatgg taggagttgc aaaaggatat
     1201 aaagtaatca tcgtgatgcc tgagacgatg agtatcgaaa gacgtttatt gatgaaaggg
     1261 tacggcgcag agttgatttt gactcctggt gcggatggta tttctggatc aatcagagaa
     1321 gcagaacgtt tggcaaaaga aaacggctac tttttaccat tacaatttga aaatgaagca
     1381 aatcctcttg tgcatgagaa gacgactggt cctgagatac atcaagcatt tggagtaaat
     1441 ggcttggacg cgtttgttgc aggtatcggt acaggaggaa cgatcactgg cgctggccga
     1501 gaattaaaac gtgtgtatcc gaaaatcgaa ttaatcgggg tagaaccagc agaatctgct
     1561 attttagaag ggaaagaagc gggacctcac aaaatccaag gaatcggtac aggttttgtt
     1621 cctaaaacac tggatacttc tgtgtatgat aaagtccttt ctattagtgg agacgaagcg
     1681 atggaaacag ctagagaagt tgggcgaaaa gaagggattt tagtaggcat ctcatctggc
     1741 gctgcaattg ctgccgcgct aaaagtcgca aaagaattag gaaaaggcaa gaaggtatta
     1801 gctgtggttc ccgacaatgg tgaacgttat ttatctacag ccctttacca agagatataa
     1861 ggcacttggt ttttctccag ataggtttat gtaaaaaact tttaaagatg aaaagaacac
     1921 acaaaaattt atgatgagaa aaattatttg aatagagacg taaaatagtg aacgcgactg
     1981 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
     2041 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
     2101 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
     2161 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
     2221 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
     2281 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
     2341 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
     2401 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcgagca gactgactga
     2461 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
     2521 agcaaaaaag gcgcaaatgt aaaaacagtt tctataagtg ttatacctaa aaaaagctag
     2581 ctgtgtaaaa aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatatc
     2641 aaagtgaata ttatcacaat aagaatcttg tagatattgg gttcttatgc ttgaaaagga
     2701 atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta tccgttttat
     2761 ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca taaatgaaag
     2821 tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt ttaaaaaatc
     2881 atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc ttatcttgcg
     2941 gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc tactcaaatc
     3001 ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta acagacgtcg
     3061 atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca gaaacggttt
     3121 cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg attagtggaa
     3181 tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa attattgcac
     3241 aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt attgaattag
     3301 tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta gatcgcatct
     3361 tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg acagaccgcg
     3421 gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa gaaggaaaag
     3481 tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt ttatgtgtgg
     3541 gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca aacggggcaa
     3601 tcaaagtgaa tgaatacatg cagacaagtg atccagacat ttttgcagct ggagactctg
     3661 cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca acaaatgccg
     3721 ttcgtcaagg tttacttgtg ggaaataact tgacagatca taaaatggcg tatcgtggta
     3781 ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca ggtgtgacca
     3841 aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa gataattatc
     3901 gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat gaaaaaggaa
     3961 cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa tcagcaaata
     4021 ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt tcagatttct
     4081 tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca caagaagcgc
     4141 taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt tgtcacgacc
     4201 ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc ttcgaatttt ttgtcacaat
     4261 aagcagcaaa gataaagctg ttacataaaa gaacaagcag ctttatttga ttttgaaaag
     4321 aaaggtgaga aagaagatga ataaagaata ttttgtccaa caagatgcat ttttagaaga
     4381 ttataaaagt gcttcgaaga aattaacgat caatattgat gagttggcag atcaagtact
     4441 ctctcagttg aatgaaacgc aagaggaata ttacacgatg ccagcaaatg acgccaaaga
     4501 taatgtttct catcgttttc ctttcagtag acgcatctac tcagatgaaa atgacggcac
     4561 attgaataca gagtacacat atgaaggtca gccatatgtg ctagaaactg atgatgatct
     4621 ttaaagacag tttttgaaaa ggccgctcat ccttaagtga tgacaaaaaa tcatcacaat
     4681 taccgatgac tgtctcttta ctatctttta atcagaaaaa ggggttatga caagctttgt
     4741 caaccccttt ttttcttggt ttcagattgg gttttcctac aaaatatttt tatctttgtt
     4801 gaataggaaa caaaaaaaca atataatgaa agaaaaagaa ggtgtagaaa tggataaaag
     4861 acaactgcta atagaagaat atctggattt agctaatgag attattcatc gtaataaacc
     4921 agaaatggaa aaagaatttg caggatttac tctaaatgaa ctagaagtga ttgaacatat
     4981 cgggaaaatc cctgatccta acgtgacaaa attagcgaat gctagttaca tgactagagg
     5041 agccatcagt aagctaacaa aaaaactgat agctagaggg atcataaaca cttatcaaag
     5101 cgaagagaat aaaaaagaaa tctattttca attgacgcca atcggacaaa aaataaggaa
     5161 tagacacgag caactccatc aaaaatgggt acagcgagat gctactgtat ttgaaaatat
     5221 gtcaaaagaa gaatttgaca cagtatttcg ttttatcggt cgatacagag aatttatacg
     5281 aagtagcaaa tgaagctact attttttatt gtagtgataa taggcaaaaa tcataagcaa
     5341 aaatccgaat accgtcggaa gaaatggtgc tgcagcataa agctgtcctg cgattagcgg
     5401 gccgatgacg cgcgatagag attgtatact ttgggtaccg cccattacaa gaccttgctg
     5461 gttatctgag acagaattgg atagttggcc attgaataca ggagtaaaaa tagaatcacc
     5521 gaaaccataa cagataatcc caaataaaaa caaaatcggc agtagaagaa ttccagataa
     5581 tgtgatgaat agataggcga ggatttcact gaacatccct attcttgtga tctgctgttc
     5641 tgaaaatttt ttgagcaatc gaggcataat gaacaattgc gagacagtat ccaagattcc
     5701 aatcaaagag aagcttaaac caaccaaccc agccttccac tgaaaggcgt caatcgaaaa
     5761 ttgagaaaag atagattgaa gagagccagc tgccaaccaa acagcaaaac ctgctgtcaa
     5821 taaaggaata acggatgaaa tcttgaataa ttgttgtaat tggtgaaaag gtcttacatg
     5881 ggagaaactt aaatctactg aacgtttttt tattggaagg ctttctagca taaatgtgta
     5941 gccataaacg gcatttaaga aagtaaagag tgctccaata aatataggaa cagaattccc
     6001 caactcagct aatagacccc ctataatagg acccagcgtt gttcctatac cgactaaagc
     6061 acccatccat ccaaaaactt tggttctttc attaggttcc gtgatgtcgg caaagtaagc
     6121 atataaggtg gagatttctc ctgccgtcaa tccttcaatg atacgaccaa ggaacaacat
     6181 ccatatcgaa tttcctaaac caaaaagcaa atatccgata cttgatccta ataaactgat
     6241 aataaggatc ggatgacgcc caaaatgatc acttaagcta ccgagaatgg gggcagataa
     6301 gaaggcagct agagcataaa cagacatgag caaagtgata tagaaagctt gctgatgtat
     6361 ggagtgagaa taaggcagcg ttaaaaacgg aagtacagga ctgacgatag taaatcctaa
     6421 tccagtgaat aagacagaga cgtatccaaa aactttagaa tgtttatttg tgaaactgta
     6481 catgtaaaaa ctcctttatg caataatgtt aacttggaaa caaaaatagt gtatagtaat
     6541 tttgtttcct tgtcaacatt ctaaggaaca gcgaaatcta cttaatttat ttattccgaa
     6601 aagtgaagat acgcatcaaa aacatgaaca aacaaaaatc aagtagacga cattgtaaat
     6661 ctgaccgatc agtgaagtaa atagaaaaga aacctgttct tgcttcccat gtttttttta
     6721 cgtatactgt gattgagcgg aggagaaatc atgttgaatt tagaaaatta cttaagcgct
     6781 ggacaaacag tcatatctaa tcttttactt aaaaattatc ataggatcgg tctacaatct
     6841 gaagaatttt tatttgttct acagttgcat atggcacagc ttgaaggaga tactttccct
     6901 gatttgcaga tgattgctat ggatatggga atcaaacaag atcagatttt tcaaatactg
     6961 gatcgtctcg taacaaatgg atttatcaag atagaaacga cgattgtcaa cggtaaaaaa
     7021 gcagatcgat ataatcttta ccccatctat gatctattag gtgagtattt aaaaacacaa
     7081 gaaaaaaaat acgaagaaaa aacacaagaa aaagaaatcc agtcagtgta tcaattattt
     7141 gaacaagaat ttggacgccc gctttcttcg atcgagttcc agcggatcgg acaatggcta
     7201 gaagaagacc attatcagcc ggaaattttg aagctagcac tgcgggaagc cgtattaaat
     7261 caggcttata gttttaatta cgttgaccgc attttgctat cgtgggaaag aaaaaatcta
     7321 cgtacaaaac aacaagtgga agaagaccaa aaacgaagaa aacagcagct tcttcaaaga
     7381 gaagtagaac agtcagctca acaagaagaa ttgccgaaag tctcactgaa aaattggttg
     7441 gaggagtagc ggaatatgtt aagcaaacaa aaaacaatgg aagctctaga aacgatgtat
     7501 ggaatgtttc ccgaagcaca tggagaactc aagcacaata atccatttga gttattgatt
     7561 gcagtgatct taagtgccca ggcgacagat gtatcagtga ataaagcaac gccagatttg
     7621 tttgcctctt ttccgacacc cgatgcgtta gcagaagctt caatcgatga aatcattctt
     7681 aaaataaaaa cgattggttt gtatcgaaat aaagcaaaaa acatcaaagc gtgtgcacag
     7741 cagctgattg aacgtttcga tgggcaagtg ccaacgtcaa gagaagaatt gatgtctctt
     7801 cctggtgtag gcagaaagac agcaaatgtc gtattggggg atgcattcgg gataccagca
     7861 atcgcagtag atacccacgt ggaacgtgtc tcaaaacgcc tgaggatctg caaactagat
     7921 gctacggtca tggaagtaga agaaacactg atgcggaaag taccacaaga attatgggta
     7981 aagacacatc atacactgat tttttttggt agatatcatt gcacggcaag aaatcccaag
     8041 tgcgaggtat gtcctttgct atcaatatgt caagatggga aaaatcggat gcgtctaaaa
     8101 gaaaaggcgc taaaaaagaa accacgcctg tagacgtggt ttctttaatt gcaaataaat
     8161 gtatgaatta tcttgttatt atggagaaaa gctgtttcgt ttatcttttt tgcatgaaaa
     8221 agtaaatgta catactcagt agctaggatg gtaacttatt acaatttatt gcgataaaaa
     8281 taaaagttgt tatttttatt gggaaaatac ccgatgtaga aaataatgcc ttccaatata
     8341 cctataaaat aagtccagta ttccaaagga ttatataaat aagtgacgta tctatctaaa
     8401 ggtgtaataa aaatgataag tgataataaa aaaataccat ttataactaa acataaacta
     8461 atggatttgg gcggtttcag gctcatcaga aaaatataga ttctggtgaa caacaaccat
     8521 aaaaaagtga gaatcattgc atgagagctt ctttccataa attttccttt aagagtagtg
     8581 cttaaaagta cagaaaaaat aataaatgca aggataggag caattaaagt aaatttattt
     8641 aataataaac aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat
     8701 aaaaaaagat ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg
     8761 aagccttctt tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca
     8821 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
     8881 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
     8941 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
     9001 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
     9061 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
     9121 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
     9181 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
     9241 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
     9301 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
     9361 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
     9421 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
     9481 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
     9541 ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg
     9601 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
     9661 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
     9721 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
     9781 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     9841 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     9901 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     9961 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
    10021 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
    10081 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
    10141 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
    10201 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
    10261 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
    10321 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
    10381 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
    10441 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
    10501 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
    10561 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
    10621 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
    10681 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
    10741 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
    10801 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
    10861 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
    10921 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
    10981 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
    11041 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
    11101 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
    11161 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
    11221 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
    11281 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
    11341 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
    11401 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
    11461 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
    11521 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
    11581 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
    11641 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
    11701 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
    11761 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
    11821 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
    11881 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
    11941 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
    12001 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
    12061 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
    12121 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
    12181 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
    12241 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
    12301 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
    12361 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
    12421 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
    12481 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
    12541 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
    12601 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
    12661 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
    12721 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
    12781 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
    12841 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
    12901 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
    12961 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
    13021 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
    13081 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
    13141 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
    13201 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
    13261 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
    13321 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
    13381 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
    13441 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
    13501 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
    13561 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
    13621 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
    13681 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
    13741 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
    13801 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
    13861 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
    13921 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
    13981 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
    14041 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
    14101 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
    14161 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
    14221 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
    14281 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
    14341 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
    14401 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
    14461 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
    14521 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
    14581 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
    14641 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
    14701 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
    14761 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
    14821 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
    14881 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
    14941 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
    15001 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
    15061 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
    15121 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
    15181 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
    15241 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
    15301 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
    15361 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
    15421 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
    15481 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
    15541 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
    15601 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
    15661 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
    15721 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
    15781 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
    15841 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
    15901 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
    15961 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
    16021 attttatcgt ttattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
    16081 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
    16141 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
    16201 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
    16261 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
    16321 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
    16381 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
    16441 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
    16501 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
    16561 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
    16621 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
    16681 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
    16741 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
    16801 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
    16861 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
    16921 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
    16981 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
    17041 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
    17101 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
    17161 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
    17221 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
    17281 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
    17341 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
    17401 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
    17461 attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt
    17521 gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa
    17581 attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat
    17641 aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg
    17701 tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca
    17761 tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat
    17821 tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac
    17881 acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt
    17941 ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat
    18001 acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa
    18061 agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa
    18121 aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca
    18181 atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag
    18241 aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa
    18301 tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt
    18361 gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga
    18421 aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg
    18481 gtgttttctc catggatgga gacattgcac gattgccgga aattattccg attgttgaaa
    18541 aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg
    18601 caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat
    18661 cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt
    18721 tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag
    18781 ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg
    18841 aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg
    18901 aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga
    18961 agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga
    19021 caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag
    19081 tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt
    19141 cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta
    19201 gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca
    19261 aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc
    19321 gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt
    19381 aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca
    19441 tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa
    19501 cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac
    19561 gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac
    19621 tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga
    19681 ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc
    19741 ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc
    19801 aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta
    19861 tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt
    19921 tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc
    19981 tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc
    20041 aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt
    20101 ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa
    20161 attggtagag acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat
    20221 tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa
    20281 gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt
    20341 atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca
    20401 ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca
    20461 ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat
    20521 atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt
    20581 tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta
    20641 tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg
    20701 ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc
    20761 aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac
    20821 ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg
    20881 ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga
    20941 gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc
    21001 atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc
    21061 gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga
    21121 agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt
    21181 actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc
    21241 tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc
    21301 atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt
    21361 gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa
    21421 cctgatttgt ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc
    21481 attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg
    21541 gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta
    21601 aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg
    21661 aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt
    21721 tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt
    21781 tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc
    21841 aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta
    21901 gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga
    21961 ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta
    22021 gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca
    22081 aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg
    22141 aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata
    22201 agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt
    22261 tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga
    22321 atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt
    22381 ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc
    22441 ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga
    22501 caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt
    22561 atttttggaa ccagctttta atgttttatt actcatacca tttggcattt atttaagata
    22621 ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt
    22681 tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga
    22741 tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt
    22801 atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa
    22861 gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct
    22921 tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc
    22981 tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac
    23041 caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga
    23101 acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg
    23161 tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt
    23221 gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt
    23281 aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca
    23341 cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag
    23401 caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc
    23461 acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc
    23521 aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg
    23581 aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc
    23641 tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag
    23701 tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc
    23761 ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct
    23821 gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa
    23881 tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac
    23941 attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct
    24001 gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata
    24061 catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga
    24121 aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac
    24181 aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat
    24241 tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc
    24301 aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc
    24361 gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga
    24421 aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt
    24481 aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga
    24541 aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata
    24601 cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat
    24661 tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg
    24721 ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac
    24781 tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg
    24841 tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc
    24901 ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt
    24961 catttcagct actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc
    25021 ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc
    25081 cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg
    25141 atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc
    25201 aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat
    25261 ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga
    25321 tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg
    25381 aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa
    25441 aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata
    25501 gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt
    25561 tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt
    25621 cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg
    25681 aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga
    25741 catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc
    25801 aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca
    25861 agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca
    25921 gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa
    25981 aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat
    26041 ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat
    26101 gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc
    26161 ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac
    26221 gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa
    26281 atctggaact tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt
    26341 cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat
    26401 gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta
    26461 agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca
    26521 gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg
    26581 agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt
    26641 attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat
    26701 tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa
    26761 cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta
    26821 aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat
    26881 ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat
    26941 gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact
    27001 gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc
    27061 attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct
    27121 cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat
    27181 gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc
    27241 gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa
    27301 gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc
    27361 aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta
    27421 cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac
    27481 tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac
    27541 gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa
    27601 aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat
    27661 attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc
    27721 ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct
    27781 gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta
    27841 gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa
    27901 cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa
    27961 caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca
    28021 caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa
    28081 aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg
    28141 gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc
    28201 gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa
    28261 aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag
    28321 ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg
    28381 gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa
    28441 tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata
    28501 aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc
    28561 gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt
    28621 aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg
    28681 cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta
    28741 tgccatgcca acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt
    28801 tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa
    28861 taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt
    28921 cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt
    28981 agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga
    29041 agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc
    29101 gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa
    29161 ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac
    29221 tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac
    29281 agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt
    29341 ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag
    29401 tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg
    29461 caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt
    29521 tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa
    29581 aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt
    29641 ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt
    29701 ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact acactggctg
    29761 tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt
    29821 ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg
    29881 aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg
    29941 cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac
    30001 gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga
    30061 ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact
    30121 tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa
    30181 ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa
    30241 aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag
    30301 aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt
    30361 ttgatcacaa ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt
    30421 aatttaaaga gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc
    30481 tgctccttta tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag
    30541 aagttcttgg attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc
    30601 gaccgtagaa actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc
    30661 gtttttcttt agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt
    30721 ccctatagaa agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc
    30781 acctccacta ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa
    30841 aagagattct cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg
    30901 gtgagaggtt agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc
    30961 atctattggt cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac
    31021 acaggtcctt attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta
    31081 ggtttgcggc gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt
    31141 tggaaagacc gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg
    31201 gtgcaaatga tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat
    31261 caagtagtcc tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat
    31321 ccttatacga aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt
    31381 tacaggaaaa gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg
    31441 tttatctatt catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca
    31501 gcttgtgttc ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa
    31561 gttttgaatc cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca
    31621 taactgataa agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa
    31681 gcactcacga cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta
    31741 cgtcaaataa attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa
    31801 ttggcatatc ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca
    31861 aatgaagaat gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc
    31921 tagtaaatta gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat
    31981 caatttttct ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac
    32041 ttccatcaat ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga
    32101 cgcgctcaat aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc
    32161 tgtgatggga atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga
    32221 agatatcaac ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt
    32281 atctgctctt ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg
    32341 agtgatttgg aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg
    32401 cttgggtggg ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag
    32461 cgaaatcatg gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc
    32521 aaggatcgtt gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc
    32581 agaaggcgct ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat
    32641 ttacggaaca ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa
    32701 tagtatttta gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg
    32761 atttggtgca gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa
    32821 agcgccggat gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat
    32881 gaaaaaagat gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt
    32941 aaagcgccat atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga
    33001 attcgtaaca gatacagaca atgaactcac actgttggag cacttgtgtg aaaatcaggg
    33061 gattcttgcc aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc
    33121 aaaagctgta gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct
    33181 agaagaaacg atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg
    33241 tgttgttttc agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga
    33301 caaactgccg atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct
    33361 aggtgcaccg gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg
    33421 atttttagta gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc
    33481 tgcattgaat atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt
    33541 ataatctacc atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga
    33601 attaattttg tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa
    33661 gcataatagg agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg
    33721 ggtagctcga ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa
    33781 aagataacgc ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa
    33841 agccccgttt atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg
    33901 attcttcaaa tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa
    33961 aatttatttt tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa
    34021 aaccgggcat atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag
    34081 tttcaatcga atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt
    34141 taccgaactt gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga
    34201 tgatctttta cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga
    34261 ttttgtcatt gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga
    34321 actgattcag cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca
    34381 agatcttccg ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc
    34441 gttatacaat aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct
    34501 agcaaaagaa agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat
    34561 catccaaaac ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga
    34621 ggttgaagcg atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg
    34681 gaatcccgat ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga
    34741 aaaggattga ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg
    34801 atcagttggt caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta
    34861 taccaggtat tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag
    34921 aagggaagat gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat
    34981 tgatgattaa aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc
    35041 tagctggtgc gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt
    35101 tccagacaga ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg
    35161 gagtgatttg tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac
    35221 agaacttaaa acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt
    35281 attcaccgat tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt
    35341 caatggacag ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt
    35401 gatccccgaa ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta
    35461 cgaagatgaa gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc
    35521 aggtcatacg catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac
    35581 gatcaacggc gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact
    35641 gctaatggtt gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa
    35701 gacctctttg cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac
    35761 catcaatgcg ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga
    35821 tgggaagtcg gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt
    35881 tgaattaaaa ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg
    35941 ttatccgtta gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca
    36001 gtttctccat gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt
    36061 tgaagcacct cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc
    36121 attttaattt agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc
    36181 ctttaagttt agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca
    36241 taagtagatt gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa
    36301 acattcctcc tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa
    36361 aatgcaagca aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac
    36421 ttatgagatt atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac
    36481 acgaggaatc tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga
    36541 tattcctgta ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga
    36601 agaaccggaa cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt
    36661 gattgatgat gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga
    36721 ttttggcaga ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt
    36781 gccaatccgt gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt
    36841 agtagagatg caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta
    36901 gaggagagag aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata
    36961 tccacgatcg cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga
    37021 tgttcggagc aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag
    37081 tcagttcagg actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt
    37141 acttaggaag cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt
    37201 atccagcgat tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga
    37261 tcatcaaaaa gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc
    37321 cagtcatcat ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata
    37381 atcagggcac gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa
    37441 ttttcttcaa catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta
    37501 ttagcggata catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg
    37561 ctgcctggtt tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat
    37621 atcttggagc aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg
    37681 gccatcttat ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc
    37741 ataagacgtt gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc
    37801 ctgtaacaag ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt
    37861 ttgtgatcgg tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg
    37921 ctttgatttt aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag
    37981 taattgccgc aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga
    38041 aaaatctgtt gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag
    38101 taggcacatt tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc
    38161 ttatcttacc agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca
    38221 aatcagaacg tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag
    38281 ttatgggctt gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa
    38341 agcgccagta ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct
    38401 tcgatgtagc ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct
    38461 ctgtccaaaa aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag
    38521 acgtagcggt tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa
    38581 caatccagtg ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct
    38641 tgcttgattt gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca
    38701 tcgtaggtga tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc
    38761 taggcgcaca agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt
    38821 acggacatta tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc
    38881 gggtgcaaca cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg
    38941 aacatgggtt aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc
    39001 cagcaccagt taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc
    39061 ggatcgttca gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc
    39121 taaatggaaa agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc
    39181 agatcaatac acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa
    39241 tcaaagcaat cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg
    39301 gacaattgat cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta
    39361 catacaagga gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg
    39421 tatgcgcgat gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat
    39481 atgacatcat tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa
    39541 atctgcgaag tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt
    39601 tcacaaatga cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag
    39661 ctgcaaaaaa caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg
    39721 gcgttatgca cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa
    39781 ccgagtcttc tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc
    39841 atgtttgcca tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag
    39901 gaatccacgt aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc
    39961 ccgaagattt cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag
    40021 ctttgatcga aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc
    40081 atggattaga agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg
    40141 aaacagcgtt tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac
    40201 aagtgatcaa ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc
    40261 taacggttgg tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg
    40321 ataaagaaga ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag
    40381 gcgaaacaca aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt
    40441 atggaacgtt tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca
    40501 gatgctagtg tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact
    40561 atcactgatc aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat
    40621 tatggggtaa atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta
    40681 aaagaacatg cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg
    40741 aaacaaaaag gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc
    40801 cgtgaagtcg gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca
    40861 tttgatcagc tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac
    40921 aaaccttatc caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa
    40981 catagtattt tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac
    41041 acagatgccg agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct
    41101 ggtgatccaa aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta
    41161 ccgatttttg gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc
    41221 tataaaatga aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga
    41281 agaattgatt ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca
    41341 gaaaaactca tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac
    41401 agagattatc ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat
    41461 gcactacatt tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca
    41521 gatgccgaaa cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat
    41581 tggtcaagcc gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga
    41641 aggatatgaa gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt
    41701 ggcagatcaa gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa
    41761 agagcgtcca gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat
    41821 ggagctggca gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct
    41881 ttctgcgatc gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa
    41941 tcagccaatc cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa
    42001 taaaatcggc tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg
    42061 tatgtgtgaa aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc
    42121 cgctacccaa tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt
    42181 gatgagagat tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt
    42241 gggtatccat acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga
    42301 atatcaaatg cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg
    42361 ctgtaatgtc caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa
    42421 tccgcgtgtt tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa
    42481 gctagctgct aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg
    42541 aacgactttt gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg
    42601 gccgtttgat aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg
    42661 tgaagtgatg gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt
    42721 agaaatcggt acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct
    42781 aatccaaaaa gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag
    42841 aagagggtac accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga
    42901 taaactgttg catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa
    42961 gatattgaaa acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga
    43021 aataaacgaa gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa
    43081 gatggttgac acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta
    43141 tgaatatgaa aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg
    43201 tcctatccgt atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc
    43261 aatccagcag gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac
    43321 tgacttttct atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa
    43381 tgtgatcgag ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat
    43441 caacttagcc gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga
    43501 cttggatcgt gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc
    43561 tcaaccttta ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat
    43621 tggttatcca gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt
    43681 tgaaaaccag cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca
    43741 tccagtacta gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga
    43801 cggtgaaaca gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc
    43861 aggggattca atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat
    43921 cgaagattac acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca
    43981 gtttgtcatc catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac
    44041 tgttccattc ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat
    44101 tttgggagag aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga
    44161 agttcatgtt aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt
    44221 agggcctgaa atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc
    44281 tttgtacaag gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt
    44341 tacgattgcg gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat
    44401 tggctatagt ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt
    44461 cgttgaaaca gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat
    44521 aagagatggt aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc
    44581 taaagacggt ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc
    44641 tttagacaca gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc
    44701 tatttaacgg cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag
    44761 atgaaacaag aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa
    44821 atgacattga aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt
    44881 ctcgtgccgc gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac
    44941 gaaacaaata cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct
    45001 actctttctg ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg
    45061 tccaatcttc atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct
    45121 ctatatgagt tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt
    45181 tttgccacac atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga
    45241 atcgctactg acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga
    45301 acgaagacgg aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg
    45361 gaacagctgt tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt
    45421 gggatagggg cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa
    45481 agtgtgaaag tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg
    45541 agaatccttt agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa
    45601 gcggctgttt cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat
    45661 cgatcatggt caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg
    45721 cggaaacacc atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg
    45781 ttatgcataa acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg
    45841 ttgctggtgc ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa
    45901 atgtgaaagc aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt
    45961 ttggaacaga ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg
    46021 ttcctgtcta tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa
    46081 tcgaagctgg aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg
    46141 accttaagac aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa
    46201 tcaagcctgt cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca
    46261 ttgggatggg cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa
    46321 gtgctgtagc tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta
    46381 aagagctccc tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag
    46441 aagtgagaga ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga
    46501 tttttcaact aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt
    46561 gtttgtgaaa atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct
    46621 aaaagaggct ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt
    46681 cgaaaaagca atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc
    46741 tggcggtatt cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg
    46801 tggaaaacgt ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat
    46861 gcatgccgat caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg
    46921 tgccaaaaaa gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa
    46981 agaaatggca ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt
    47041 cgcaggagat cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt
    47101 tatcgttata ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat
    47161 tcaaacagaa tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt
    47221 agaaattgaa gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa
    47281 aagcccaatt tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat
    47341 cgcaaaagga ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg
    47401 aactgcaaca gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat
    47461 ggtctatatt cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat
    47521 atttgaaggt cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt
    47581 ggaagcagct gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt
    47641 tacgtatgag ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac
    47701 gttgaccaat tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca
    47761 agaattaact ctgctaaaag aatggaaaaa agatcctgaa aattggcaga tttaagcgcg
    47821 cttgatgaag atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa
    47881 atccaaaaac tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt
    47941 gatcagtttt tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga
    48001 ataatctctt ctcttttttc tattatatat agattttctc tattttttgg taagggagct
    48061 gtttcaaaaa tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat
    48121 tcggaaaaag aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg
    48181 attgaacaat ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac
    48241 agttgcagca cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga
    48301 agcgaatgga caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta
    48361 cacagagcgt tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag
    48421 agggatgctg caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc
    48481 agctgagttc caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga
    48541 aggagaacta ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat
    48601 catcaaaaat aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat
    48661 taaaagcttt gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact
    48721 attgaaccag gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa
    48781 gaaagaagaa aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat
    48841 ggatgtcgaa tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga
    48901 ttggtttgcg agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga
    48961 agaaaccaaa atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga
    49021 caaggaattc aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt
    49081 caagaaagaa cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat
    49141 caacggaaaa caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa
    49201 tctagttgtg ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga
    49261 aaatggggaa agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt
    49321 tccggagcaa aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg
    49381 gccaatccaa tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc
    49441 cttcgatggt cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga
    49501 aatttattat ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct
    49561 gtagcaaaca atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt
    49621 ctttcagctg gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg
    49681 aatcgagggg catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata
    49741 ttggtccagc ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt
    49801 tcgatcgctg cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa
    49861 ttcaaagacg agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa
    49921 gaagtcaaag agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct
    49981 ggtatcagaa gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa
    50041 acgcatattt ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa
    50101 gaattgttat cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt
    50161 tatgaagacc ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt
    50221 actttgatga atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt
    50281 ggagggattc gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt
    50341 tatatcattt attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag
    50401 cttactcggt tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa
    50461 caccgttgat caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag
    50521 caaacaattt ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac
    50581 tcattgatta cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa
    50641 tcgtcttttg gttttcataa ataacataac caggcttggc gccatttggt tttcggatat
    50701 gctttacctg tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg
    50761 cagctaactc ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga
    50821 cgtgcgaacc gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca
    50881 ttgtcagctg gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag
    50941 aaaagtattg atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt
    51001 tcaggtatcc ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa
    51061 tttccagttg agataagaca gattcaagat attggatttc atctttggct tcttggattt
    51121 gttcgccaat cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg
    51181 cattttgatt tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat
    51241 aataattagg taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa
    51301 gtagttcgcc atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac
    51361 gttttttcaa tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc
    51421 cgccttgttg ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt
    51481 gactgagtgt tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa
    51541 tcggtgtgaa aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga
    51601 tggctgagaa aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt
    51661 tggtcagctc ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg
    51721 gatctagttc agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt
    51781 tcaagacctc ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag
    51841 tgttttgaga gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa
    51901 ctaagacgat cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat
    51961 cacccaactc gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt
    52021 cgatgtcttc taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg
    52081 gattatcagg attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg
    52141 cagataataa taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt
    52201 cataaggctg atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga
    52261 ccatcgcgtg ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa
    52321 gtatattcat tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa
    52381 gtctagaaaa atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa
    52441 tttttaggta
//
LOCUS       DACIWG010000018        51540 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.018, whole genome shotgun sequence.
ACCESSION   DACIWG010000018 DACIWG010000000
VERSION     DACIWG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.018"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            476..1339
                     /locus_tag="IYF54_001034"
     CDS             476..1339
                     /locus_tag="IYF54_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9831377.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQAIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="IYF54_001035"
     CDS             complement(1468..3090)
                     /locus_tag="IYF54_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9831378.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="IYF54_001036"
     CDS             3339..3860
                     /locus_tag="IYF54_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831379.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IYF54_001037"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="IYF54_001037"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ9831380.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="IYF54_001038"
     CDS             5941..6759
                     /locus_tag="IYF54_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ9831381.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="IYF54_001039"
     CDS             complement(6862..7059)
                     /locus_tag="IYF54_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831382.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="IYF54_001040"
     CDS             7228..7806
                     /locus_tag="IYF54_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ9831383.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8852..9355)
                     /locus_tag="IYF54_001041"
     CDS             complement(8852..9355)
                     /locus_tag="IYF54_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ9831384.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9714..10739
                     /locus_tag="IYF54_001042"
     CDS             9714..10739
                     /locus_tag="IYF54_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9831385.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11086..11964
                     /locus_tag="IYF54_001043"
     CDS             11086..11964
                     /locus_tag="IYF54_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831386.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12440..12556
                     /locus_tag="IYF54_001044"
     CDS             12440..12556
                     /locus_tag="IYF54_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ9831387.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13484..13822
                     /locus_tag="IYF54_001045"
     CDS             13484..13822
                     /locus_tag="IYF54_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831388.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13916..14146
                     /locus_tag="IYF54_001046"
     CDS             13916..14146
                     /locus_tag="IYF54_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831389.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14546..14917
                     /locus_tag="IYF54_001047"
     CDS             14546..14917
                     /locus_tag="IYF54_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ9831390.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15191..15883)
                     /locus_tag="IYF54_001048"
     CDS             complement(15191..15883)
                     /locus_tag="IYF54_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9831391.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15886..16671)
                     /locus_tag="IYF54_001049"
     CDS             complement(15886..16671)
                     /locus_tag="IYF54_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9831392.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16908..18308
                     /gene="lacG"
                     /locus_tag="IYF54_001050"
     CDS             16908..18308
                     /gene="lacG"
                     /locus_tag="IYF54_001050"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ9831393.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18333..18650
                     /locus_tag="IYF54_001051"
     CDS             18333..18650
                     /locus_tag="IYF54_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9831394.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18663..20336
                     /locus_tag="IYF54_001052"
     CDS             18663..20336
                     /locus_tag="IYF54_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9831395.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20350..21981
                     /locus_tag="IYF54_001053"
     CDS             20350..21981
                     /locus_tag="IYF54_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ9831396.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22853..24043
                     /locus_tag="IYF54_001054"
     CDS             22853..24043
                     /locus_tag="IYF54_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9831397.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYSPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            24177..26366
                     /locus_tag="IYF54_001055"
     CDS             24177..26366
                     /locus_tag="IYF54_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9831398.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26363..28156
                     /locus_tag="IYF54_001056"
     CDS             26363..28156
                     /locus_tag="IYF54_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9831399.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28356..28670
                     /locus_tag="IYF54_001057"
     CDS             28356..28670
                     /locus_tag="IYF54_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9831400.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28691..29068
                     /locus_tag="IYF54_001058"
     CDS             28691..29068
                     /locus_tag="IYF54_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9831401.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            29078..29851
                     /locus_tag="IYF54_001059"
     CDS             29078..29851
                     /locus_tag="IYF54_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831402.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29873..31276
                     /locus_tag="IYF54_001060"
     CDS             29873..31276
                     /locus_tag="IYF54_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ9831403.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31458..32642
                     /locus_tag="IYF54_001061"
     CDS             31458..32642
                     /locus_tag="IYF54_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ9831404.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32639..32929
                     /locus_tag="IYF54_001062"
     CDS             32639..32929
                     /locus_tag="IYF54_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831405.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32926..33147
                     /locus_tag="IYF54_001063"
     CDS             32926..33147
                     /locus_tag="IYF54_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831406.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            33189..33968
                     /locus_tag="IYF54_001064"
     CDS             33189..33968
                     /locus_tag="IYF54_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ9831407.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            34035..34529
                     /locus_tag="IYF54_001065"
     CDS             34035..34529
                     /locus_tag="IYF54_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ9831408.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34585..35223
                     /locus_tag="IYF54_001066"
     CDS             34585..35223
                     /locus_tag="IYF54_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831409.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35295..35690
                     /locus_tag="IYF54_001067"
     CDS             35295..35690
                     /locus_tag="IYF54_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9831410.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35674..38127
                     /locus_tag="IYF54_001068"
     CDS             35674..38127
                     /locus_tag="IYF54_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9831411.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            38124..40151
                     /locus_tag="IYF54_001069"
     CDS             38124..40151
                     /locus_tag="IYF54_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831412.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            40148..41170
                     /locus_tag="IYF54_001070"
     CDS             40148..41170
                     /locus_tag="IYF54_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ9831413.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            41187..42116
                     /locus_tag="IYF54_001071"
     CDS             41187..42116
                     /locus_tag="IYF54_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9831414.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42361..42477
                     /locus_tag="IYF54_001072"
     CDS             42361..42477
                     /locus_tag="IYF54_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ9831415.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            42493..43296
                     /locus_tag="IYF54_001073"
     CDS             42493..43296
                     /locus_tag="IYF54_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ9831416.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            43471..43767
                     /locus_tag="IYF54_001074"
     CDS             43471..43767
                     /locus_tag="IYF54_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831417.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            43774..45726
                     /locus_tag="IYF54_001075"
     CDS             43774..45726
                     /locus_tag="IYF54_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ9831418.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(45798..46295)
                     /gene="dfrG"
                     /locus_tag="IYF54_001076"
     CDS             complement(45798..46295)
                     /gene="dfrG"
                     /locus_tag="IYF54_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ9831419.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            46657..47697
                     /locus_tag="IYF54_001077"
     CDS             46657..47697
                     /locus_tag="IYF54_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9831420.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            47795..47983
                     /locus_tag="IYF54_001078"
     CDS             47795..47983
                     /locus_tag="IYF54_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9831421.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(48041..48394)
                     /locus_tag="IYF54_001079"
     CDS             complement(48041..48394)
                     /locus_tag="IYF54_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9831422.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            48925..49347
                     /locus_tag="IYF54_001080"
     CDS             48925..49347
                     /locus_tag="IYF54_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9831423.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            49344..49574
                     /locus_tag="IYF54_001081"
     CDS             49344..49574
                     /locus_tag="IYF54_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831424.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            50072..50272
                     /locus_tag="IYF54_001082"
     CDS             50072..50272
                     /locus_tag="IYF54_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9831425.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            50299..51492
                     /locus_tag="IYF54_001083"
     CDS             50299..51492
                     /locus_tag="IYF54_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9831426.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT        17071 a         8509 c        10033 g        15927 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag gcaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaaat aacatatctc
     8341 attttacgct tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca
     8401 gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta
     8461 ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac
     8521 catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct
     8581 gagtagggta taaattaaga cggaattcct ttctagattg agatattgac taggaagaat
     8641 gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata
     8701 gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa
     8761 aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg
     8821 tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa
     8881 ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt
     8941 ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc
     9001 ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt
     9061 gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa
     9121 gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag
     9181 tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga
     9241 cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca
     9301 accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca
     9361 tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa
     9421 gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg
     9481 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     9541 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     9601 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     9661 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     9721 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     9781 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     9841 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     9901 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     9961 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
    10021 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
    10081 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
    10141 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
    10201 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
    10261 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
    10321 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
    10381 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
    10441 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
    10501 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
    10561 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
    10621 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
    10681 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
    10741 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
    10801 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
    10861 actttcaaaa aaattttata tttattctat caaaaaattt tatatttatt ctatcaaaaa
    10921 attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct ttccttgaaa
    10981 gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg tacaataaaa
    11041 gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag gttcaaagat
    11101 atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct ggcagaaact
    11161 ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa catccttgat
    11221 gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa ggcgactgca
    11281 aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat ttatctatat
    11341 gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag aactatctat
    11401 ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt aacaggaagc
    11461 tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat ctttcggatt
    11521 ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct aagattagaa
    11581 ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca gaataagata
    11641 agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga ttaccattac
    11701 caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg gttgaaaaaa
    11761 gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga tgaacaggac
    11821 gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga ttatccagaa
    11881 agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca cattggctct
    11941 ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga
    12001 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    12061 caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
    12121 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg ttctatcgaa
    12181 cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt taacggacaa
    12241 gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag agataggagt
    12301 caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac ttttgatacc
    12361 atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact acaatcaaag
    12421 aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc gccgcttgaa
    12481 aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa
    12541 tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa aacgtttttc
    12601 ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc
    12661 atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc attccctaat
    12721 tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa gtattatctg
    12781 tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg atcactttac
    12841 cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa cgtaactaca
    12901 taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga gcaatacggt
    12961 tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc tctaacgttc
    13021 agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg
    13081 aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa aaagcagtta
    13141 ctattgctag aataacatca aggaataata agttttggtt ttcagataat agtcgataag
    13201 ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt tgtgagaatc
    13261 actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag tagaatgtaa
    13321 atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga ataagttttt
    13381 caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata ctttgttgat
    13441 tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc aaccagtttc
    13501 aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat atcaggaaaa
    13561 tattgttccg gagactccat tagctagtga gccaattact gatattgatg gaaaccaaat
    13621 aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag taaaaaatac
    13681 agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt atatttatac
    13741 tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg ctactgataa
    13801 aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga aattgatgaa
    13861 aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat tcgatatgct
    13921 ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta gagaagatga
    13981 aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga catttacagt
    14041 ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg gaatgtcttt
    14101 agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga gttcttatta
    14161 tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg ttaatattac
    14221 taactttaat gcagtataaa gagtatccag agtattttat tttaactttt gtatatttag
    14281 taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct aaagtttgtt
    14341 tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt aaaaatatta
    14401 tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta ctgataatga
    14461 aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt ttcaagatta
    14521 ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa ctcaagaagt
    14581 catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag aagcagttgc
    14641 cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca attatggcga
    14701 taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg tgaaggcaga
    14761 tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg ggacagagaa
    14821 agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc atttttcaac
    14881 tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag cattaataaa
    14941 agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat aatgtgaaaa
    15001 cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta aaagaatgaa
    15061 aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct ttcatgtcgt
    15121 ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct ttggtagtta
    15181 gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa aaaccagtaa
    15241 aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt ttgtttttac
    15301 cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca agtaatcctg
    15361 agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg tctgccgctt
    15421 taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag acaatcttgt
    15481 cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca ttctctttga
    15541 cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca agagtcgctt
    15601 caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata cgataatagc
    15661 tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct gaaatgaaat
    15721 gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc tcgtctgata
    15781 aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc agatgagagt
    15841 ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg ctcctatagt
    15901 ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt gctcttgaac
    15961 ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat tataaaacac
    16021 ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct tacgggaatt
    16081 atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga tcgcttcgtt
    16141 gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta aattgtcgtt
    16201 ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa tcagtagaat
    16261 ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg taatgatccg
    16321 aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg gtcctaagaa
    16381 aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt ttttttcttg
    16441 aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt aggaaagact
    16501 ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat ctaaatctct
    16561 gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg taaggattcc
    16621 ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca ttgtcttcac
    16681 ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat tcgaacaaaa
    16741 gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg ttttctggtt
    16801 tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt acaatgcaat
    16861 cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg cttaaattac
    16921 cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga gccactaagg
    16981 aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg cgattcagtc
    17041 ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg tgtgaacgtt
    17101 ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct gatggagctg
    17161 ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa tgtaagaaaa
    17221 gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt ttgtttgacc
    17281 atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc attttttgtt
    17341 ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat ccagttgcaa
    17401 ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt acaaagatcg
    17461 tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat ttcaaagaaa
    17521 acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat gcggcaacag
    17581 acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc cgtttcttat
    17641 tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt gatgagatat
    17701 gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa ttgaaaaaag
    17761 catccacgcg taatgactac ttagggatca atcactacca atgccatttt gtcaaagctt
    17821 acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc tctgtttata
    17881 aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact gattgggact
    17941 ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc gattacccac
    18001 attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag tttgaagatg
    18061 gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa tccttaagca
    18121 aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt atggatcttt
    18181 tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat tttgaaaccc
    18241 agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa accaaaacga
    18301 ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta caaatgcttg
    18361 gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa ttattgaaag
    18421 aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc gacgaaaatt
    18481 taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct gggaaagaga
    18541 tggagatggg attcattttt atccatgggc aggatcattt aatgaccact ttattgctga
    18601 gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag gagaagaacg
    18661 ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa aaaatctcac
    18721 gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca gctattttat
    18781 tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac caatggcctg
    18841 aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt gtagggttac
    18901 tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc ttaccgaaaa
    18961 acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc ctgttgttaa
    19021 gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag ggactactgg
    19081 cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg ctgaaagata
    19141 ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc cgagatattt
    19201 ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt cgacaaatct
    19261 tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt acagcagctg
    19321 acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg tttgtcggtg
    19381 ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc aatgtggaaa
    19441 ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg gtgggtttag
    19501 ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct tttatcttcc
    19561 tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt attccagtaa
    19621 gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac ccttattttt
    19681 ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt tttgtcgatg
    19741 tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca ccgatcggtt
    19801 tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt ctaatcgtcg
    19861 ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta gtagagaaag
    19921 aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat gtacgtgagg
    19981 aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc aatgtgttgg
    20041 tactttgtgc tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa aaaggtgcat
    20101 ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat tacgaaatgc
    20161 tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat gatgatatca
    20221 aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca gaatatatcg
    20281 gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt caataaagga
    20341 ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc agctgtgtta
    20401 ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt tattcgcgga
    20461 gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta tgaagacgat
    20521 atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt tcgactgcgt
    20581 ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac aaatgatctg
    20641 gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt gctgctggat
    20701 tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa agcatggcaa
    20761 ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa cgttctgtcg
    20821 gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga actgaatagc
    20881 ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt tgatcgattg
    20941 gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa aacaactcca
    21001 gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg gtttgatgaa
    21061 atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc gtattggcac
    21121 ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta tggtaaagaa
    21181 gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac tatgccaaat
    21241 gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac aggacaagca
    21301 gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg cggggcgggc
    21361 ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac acaagcaggt
    21421 atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca agcgatgttt
    21481 gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg ggcgcaaata
    21541 aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc ttccaattgg
    21601 caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc gggcactttt
    21661 tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc agcttatcaa
    21721 aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat gtctggggga
    21781 gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact aaatactgcg
    21841 gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat tacaacaggt
    21901 gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa cgttgatttt
    21961 gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga ggttcccttc
    22021 agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt gccaaaaata
    22081 accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac ttcatctaca
    22141 ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa aaattgcaaa
    22201 aagttgtgca gataacataa agtttgttta agaaagggta accttagaat tccgtcgtga
    22261 atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt cgtttttcga
    22321 gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct cttttggtag
    22381 ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat gttgatttat
    22441 agggattccc cctccatatt atgttggctc tttattccca ctctgttttc aacgtaaagt
    22501 gtataaaaca gaatgcgata ctatcaaggg agttaaaaca atatacagaa cagtaaaaca
    22561 ctataaataa aaataaaagt gcaataaaag tgcaattttt gcacctaatc atactctgtt
    22621 tatatatgaa aactagattc tgaatccaat atttggttaa cgagaggcgt tcgaatttga
    22681 acgtctttta tatattactt tcaaataaat atgaggtaga atactatcta ttttcttttt
    22741 aacccaattg ttataatgga gagtgtaaaa tattttgtgt aaatagaaaa aaggaagtcc
    22801 cttctgtaga atagagttac cacaacacat tcacagaaga gaggacttcc ctatgaacga
    22861 ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg aattattccg
    22921 tgtccatttg gagaaagcag tcaatacgct tctcaaaacg gagttaacgg ctttcctaga
    22981 ttacgaaaag tatgatcgca ttggttttaa cacgggtaat tctcgtaacg gctcctatga
    23041 ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg accgcaatgg
    23101 tgagttcaag caacagactg ttcctgctta tagacggacg aatgacacgt tagaggagac
    23161 cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact tgattgagaa
    23221 aatgtatggg cattactact cgccccaaac catgtccaat ataacaaaat catttacaga
    23281 agaggtaacg gcgtttaatg ggcgggagct tcatgaccgt tatgctgcta tttatatgga
    23341 cgcaacgtat attccgttaa agcggaaaac cgtcgccaag gaagctattc atatcgcagt
    23401 tggcattcgc ccggacggat caaaggaagt attgagctat gcgattgcac cgactgaatc
    23461 catcacgatt tgggaggaaa ttttattgga ccttcaagag cgcggtttga aaaatgtcct
    23521 cctgttcatc acggatggct taaaggggat ggtaggagcg attagtcggt tctatcccaa
    23581 agctcgtttt caacattgtt gtgtacacgt ttcccgtaat atcagtcata aagtgcgtgt
    23641 cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcct catctaaaga
    23701 ggtggcgttg gaagcacgtg gtacttttgc ggaaaaatgg aaaaccagct atccaaaagt
    23761 ggttgaatcg attctttcga acgatcactt gctcactttc tatgatttcc ccttggccat
    23821 acgcaagagt atttattcta cgaacttgat tgaatccttt aataaacaaa tcaagaaata
    23881 cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct tagtctcgtc
    23941 ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc aacaggccga
    24001 aggcgagctc gaacaaatgc taagccaact gattgagaat tagaaacaat ctgcagtagg
    24061 gaagaatcat ttacacaaaa ttattgacgc tcccttataa tgagaacttt tactaacata
    24121 atttatgata aacttactat ataatcatat taattaaata gttaggagat ctgcttatgt
    24181 ttatatctac tcttcaaatt cgaaatttta gaaattatga atccgaaatc tttcgattcg
    24241 ataatgagac aaatactatt ataggtgaga atgattcagg gaaaaccaat gctcttactg
    24301 cattgagaat cttattagat gatagttatt actatagtag taaaacacta aaagaatcag
    24361 atttttttca tggaattcaa aatgggtggc aagggcattg gattattatc tctgcgacat
    24421 ttgaaggaat cagcgaagaa gaatttgaca atgagatttg tgcttccatt tctttaaatt
    24481 ctgaaagtca aacaatctta gaagaattga tttcaaatgc agataaaggt gtaggtagta
    24541 tttctctatt tattagacct aacaaagcta ttagaaagca attatttgat atttccactg
    24601 aattagactc tcatcaattt aacgaattta gaaattctat tcgtctatcc gactatgaat
    24661 tttactacac atctaaatca aatctaaatt tctgtatcaa tgaaaattat gataaattgg
    24721 taggaagact caatgacaat atagctagta atcctgaaga agacgatgaa gctctgttgg
    24781 gctcaagaat tgacatgtca gatattttca agcatatttc ggttgtttat gttgacgctc
    24841 ttcgagatgt tcttagagaa atgaaaaaca atcgaaaccc agtcaaaaga atcatggaaa
    24901 caattgaatc aaaaatatca tcagataatg ttgagtcatt aaaaacaatt atacagcaat
    24961 taaatgagac tattaccagt gttcctgaaa ttaggaaaat tggcgaaaat attaatcggc
    25021 aactaaatag cataattggt tcagtgtatt ctcctaatct tcttctttcc tcaactatgt
    25081 cagatgatat gggttcttta gcaaaatttc tttcaatgaa gccagaacaa aatatcgact
    25141 tagacttact agggttagga catctaaata tgatttattt agctttgaaa atagtcgaat
    25201 ttgaagcgtg tcgttcaaga gaactactta atattatgtt gattgaagaa ccagaagctc
    25261 atattcatca tcatattcaa aaaactctgt tcgaaggatt aaatcttcaa aaaaactata
    25321 ctcagattct catgaccaca cattctgtac atttagctga agtgtctgaa atatctcgga
    25381 tgaatgtatt aaaatcgttt aatggcaaat ctatcgttat gaatccagca aataatctga
    25441 actcatttgc taaagaaaag ttaaaaaaga acaatctaaa tctaataaac gcagtagaga
    25501 gatatttaga tgttaaaaga aatggattgc ttttttctaa aggtgttatc ttagttgaag
    25561 gagatgctga agaaatactt attcctcaaa tagtaaaaaa attatttggt ataaacttag
    25621 acgaacttgg aataggtttg attaatgttg gtagtacttc gtttgaatat atcgctagct
    25681 tgtttgacga tgatagaata cagagaaagt gtgctattgt aactgattta gatgaacagg
    25741 caatcccatc agaacatcga ttgtataaaa gcactgcaca agagaaaggt caagcaagaa
    25801 aagaaaaatt atcctccctt tttaatgaaa acaattgggt taattgtttt tatgctaata
    25861 caacatttga aattgagttt ttagatgtga ataaagaaaa tattctaact tatgtcagtc
    25921 caattataga tgacgcattc aaagataaaa aaacaataac tgcttataaa gaagaaatac
    25981 aaagtgaaca atatttagaa agaaatttag cgatgttaaa actagccaat catgttggta
    26041 aggggtggtt tgcaattgag ttaggtgaaa aaattgactt tttaatcaaa attcctaagt
    26101 atattcttaa tgctattgct tttgcatgtc aggaaatagt agatctctca atttataaaa
    26161 aaattctact ccatacaatc gacttgtacg aggaaactac agaattgtca gctttatcag
    26221 ctagtttaaa aacaagtgat acagctgatt tgaatatagc aataatttct ttattagagt
    26281 actttggtaa agatgaggat gttgtgtatc taatcaatca gatagaaaat tacacaaaaa
    26341 tactttatgc tgaggtttca aaatgacaaa tatcttcgaa aaaatcgtaa atgatttaaa
    26401 tttagaacaa aaagatgttg tggaaagtga agataacctt tatgtaaatg catgtcctgg
    26461 gagtgggaaa acaagggtac ttacaaggaa aattgcttac caagcaatta aacacgatgg
    26521 atcactaaaa agaataattg ctatcacata cactaataga gctgctgaag aaattaagga
    26581 acgcctaagt ttacttagta tagatgatga tattaatatt tgggtgggga caatacatca
    26641 gttttgtctt gaatttatta tttatccttt taaaatgaat ctctcaagag tatcaaaagg
    26701 atttacaatt attgatgatt atactcaaag agcttacgtc agccaaatta ataatatgct
    26761 gaatttaaat ctcaaaacgt atgaactgaa caaaattgat ttgtcacttt ctcatgattt
    26821 taaaattaat gaagttcagt atactcaagt ttctgaaatg tatcacacta tgttggaaaa
    26881 aaataaagaa attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa
    26941 cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca
    27001 agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat
    27061 tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag atggtatagc
    27121 taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca
    27181 tggctgttac aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc
    27241 ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg
    27301 caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga
    27361 aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc
    27421 actgggtgag tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc
    27481 accaattaag gtaaataatc atagtttatt tttcaagtta gcaattttgt actttactga
    27541 atcaggtgaa aaggttcgaa gaagaaagct aattgcaaac gatattttaa ttatattaaa
    27601 aaatgaattt ggaatagaag ttaaagatta ttttattgac ttagatttat tacagctaat
    27661 aaacagtgtg aaaagaatgt atagtggaga cgacaacggt gttgatttat acaaatttgt
    27721 taccaaacat atatttgtta atcttaatat tgacgagaaa aattacccgt ctatttttaa
    27781 gcactatcta ttttttatta acgaagtaga agatcggata aaaaataatt cattatctag
    27841 tagtgtatct agttttaaga agatgtttaa atctcgagat ggtatcacac ttactacagc
    27901 acataaagtt aaaggaaatg agtatgatac tgttatcgct attaatcttc taaacgggaa
    27961 aattcctcat tggaatgaaa tttttaatgt ttctgataaa ggaattagtt cagcgagaaa
    28021 actactatac gtaatttgtt caagagcaaa aaataattta tttcttttct cagaggtggg
    28081 ttattttacg agtaaaggag acttgcttta tcccactcaa ttattagata aaatagaatt
    28141 tgattataat atttaaaata cctaggtagt cgttgtttta aatatagtag gaatcggacg
    28201 ctcagtacgg agcgtctatt tttttaccca attttgaaag gaagtgaact tatgaaaata
    28261 aaaaatcaag aatcaaaagg tcgctctccc ccctttaaaa ccatcacaca aataagattc
    28321 attcaaacat aaaaacagaa aggataggta aaaatatgga acttaaatat gtcattccca
    28381 atatggaaaa aacatttgga caattagaat atgctggtga agggaacatt gaacaacgtc
    28441 gaataaatgg cagaccaacc attctctctc gtagttacaa tttatactca acgattcaac
    28501 gagcagatga tattgttgtc attcttccct ccgaagctgg cgagaaacat tttgaatccg
    28561 acgaaaaagt caaactcatc aacccacata ttaccgcaga aggttataaa attggcaatc
    28621 gaggatttac caattacatt atgcacgcag acgatatggt gaaagcataa gaaaggaaag
    28681 gtgaaaaaat atgagattag cagaaggtat tgtgattgaa aaagaccaga catttggcac
    28741 attgaaattt tccgccttgc gtcgtgaggt tcgtatccag aatgaagatg gtacggtttc
    28801 cgaagaaatt aaagaacgta cctatgactt aaaatcaaaa ggacaaggac gcatgataca
    28861 agtcagtatc cccgcttctg ttcctttgaa agaatttgac tacaacgcag aagtggaatt
    28921 aatcaatcct gtggcagata ccgtagcgac ggctactttt cgaggggcag acgttgattg
    28981 gtacgtgaaa gcagacgatc ttgtattaaa aggacagaca aaacctcatc agaaatataa
    29041 aaaagaagcg tccgaaagtg aaaaataggt gttgcttatg acaaacacac cattgatttt
    29101 gaaagaaatt ccgaaggatg aagccatttc ttttattcgg caataccatt attcaaagat
    29161 actccctaga ttgtgtaaat actttctagg gattttttca gaagaaaaat tattaggcgt
    29221 tgtggaatta ggctggggta cgcaacccct acaaaccata cgcaaactct ttcccgacag
    29281 ttcccttcaa accacagatt atttagaaat tggaaagatg tgttttcttc cagaaatgaa
    29341 tcaaaccaat tattttggca gtcaagctct ttcagctcta attaagtggt tgaaagaaca
    29401 tacggactgt cattttttat atacactggc agatggaatt gagggaaaat gtggctatgt
    29461 ctatcaagcg tccaacttct tttactgcgg gtatttcaag acaagtgtct atcgtgataa
    29521 gcagtcatgg gaaaagatac accctcgaag tgctcgcctt ttattagagg aaaatgctcg
    29581 ctttgaacag gtagagaaaa agcattggct ttcccaggca ttttgcgaat acaaagggat
    29641 tgagaaaatc aatggacgca tgtttcgcta tctctatccg ttgacaaaag aagcgaaaaa
    29701 actgttagga cacactctct atcgacgtca ttattatccg aaagaaaaaa atttacgctt
    29761 tgaaaagcga atcgcttatc agaaatatga agccatttct caaccaactt ttgataaaca
    29821 ggctcgtatc tacaatacac aattattttg ataggtggaa aggaggattt ttatgccatt
    29881 gcttcatgac agaggagagc gaatcaaagc caacgataag cacttggtct atcattttgt
    29941 actaatttca ttgctggtag tttttctagt cgtcctactc ccttttcatt ggagagcgtt
    30001 acaatcggtg aactggcaag cagttcagtt agatacattt ttctatcaac ttcatacgcc
    30061 atacttttgg agcagtattc tcactgctct ttttgtatgc ggacttatca gctggcttta
    30121 ttatcactat cgtatagatt ctataaaaca gttagagcac cgtcaaaagt tagctcgtat
    30181 gattttagaa aatcattggt atgaagccga agaaactcaa tcagagggat tctttaaaga
    30241 tttacccagc aagaaaacca aacaacgaat ccgccatttt cctaaaatat attatcgaat
    30301 gaaagatgga ctaatccata ttcatgtaga aatcacctta gggaaatatc aagatcagct
    30361 tttgcaccta gaaaacaaac tagaaacagg attgtattgt gaattagtgg aaaaaacgtt
    30421 gaaagattct tatgtagagt atgtcctact ttatgatact attggcaatc gaattggcat
    30481 tgaagacgtg gtagctcaag atggacgtgt caaattgatg gaatcggtct attgggagtt
    30541 cgattctctc cctcacatgt taattgctgg gggcactggt agtggaaaat cctattttat
    30601 tctcacattg attgaatctc tcctgcatac aaatgcaaag ctttatattc ttgaccctaa
    30661 aaatgctgat ttggcagact taggggcggt catgaataat gtctattacc gtaaagaaga
    30721 tatgttggca tgtatcaatc aattttacga agatatgatt gatcgcagtg aaaccatgaa
    30781 acaacaccct aactataaaa caggagaaaa ttatgcttat cttggtttgt ctgccaattt
    30841 tcttattttt gatgaatatg tggcaatgat ggatatgcta ggtcgtgaaa gtaccaccgt
    30901 tattcataaa ctcaaacaaa ttgtaatgtt aggacgacaa gcaggcttct ttttaattct
    30961 tgcctgtcaa cgtccagacg caaaatattt tagtgatggg attcgggaca actttaactt
    31021 ccgtgtggct ttgggtcgta tgtctgaaat gggctttggt atgatgttcg gaagcgatac
    31081 acaaaaacaa tttttcttaa aacccatcaa gggaagaggt tacgtggata caggaaaaag
    31141 tgtgattagt gagttttata caccccttgt accaaaaagg tacgatttct taggagaaat
    31201 aggaaaagtc attcaaaaaa agcaatctga gcctgtaccg cacgaagtga aaggtacagg
    31261 ttcagacttg ctttgattgg tgtggcgtta gccacgccaa tcctttagcc ccctctatct
    31321 aacagggggg cacaaataca actgaaaaca ggcaattctc atcaaccaat gttgatggta
    31381 caaaggattg cggaaaatat gaaaggtgta aactcaaggg tcatagttta catctttttt
    31441 agattggagg tttgcgaatg gaaaacgaaa aaatttggat taaggaattg aaagaaaagc
    31501 gactggctta tggcgtttct caaaataaac tggcggttgc ttctcatatt accagacctt
    31561 acctatcgga tattgaaaca ggcaaggcag tccctactca acaggtgaaa gaagatttac
    31621 tgaacgcttt ggaacgcttc aatcccgata acccattgga gatgttattc gactatgtac
    31681 ggattcgatt ccctaccaat gatgtggctc aaattatcgg tgaggtttta cgtttgaata
    31741 tggattatat gctacacgaa gactttggct attattctta tcccgaacac tatcgtttcg
    31801 gtgatattgt tgtcctagtc tctcatgatg taagtaaagg tgttttatta gaactgaaag
    31861 gaaaaggctg tcggcaattt gaaaactttc tattggctca acatcggagc tggtatgatt
    31921 tctttcaaga ttgcatggaa cataaaggta ttttcaaacg cttggattta gctatcaacg
    31981 ataaaacagg gattttgaat attccagatt tggcaaagaa atgtaaacag gaagaatgca
    32041 tttccgtctt tcgaagcttt aaaaattatc gcagtggtga attggttcac cgtgatgaaa
    32101 aaccatacat gggaaacaca ctttatattg gctcattaaa aagtgaagtc tatttctgta
    32161 tatatgagaa agattatgag caatacgtga aaaatgatgt tcccttagaa gacgcagaag
    32221 taaagaatcg gtttgaaatt cggttaaaaa atgaccgagc cacccaagcc atgaaagatt
    32281 tattggcaca tcaacaggcg gaaaaaacag cctttgaaat tatcaatcgc tatatccgct
    32341 ttgcagataa agacgacact aaacgtcgga gtgattggaa aactaatgaa cgttgggaat
    32401 ggtttatcgg taaaaatcgt ggagcattac gcctaaccac acagccagag ccttattctt
    32461 ttgaacgaac attgaactgg ttacatcatc aagtcgcccc tacattaaaa atcgcaagca
    32521 ttttagatgt tctcaacggt acaaccatta tctccaccat gattcaggaa gccaaactga
    32581 cagaaaaaca tgagaaatta attgaacaac aacatctagc catggaggat ttgattacat
    32641 gagcctttta acaaaagaat taaagaagtt agggtttcag tgtggcattg agtttcaagc
    32701 gtatatacaa aatacaggga aatatacgtc tctcattatt gagggaaaac ggcaagctgg
    32761 tgataccatc tatacctatg atttttacaa ggtgagattt tataataatt ataccaatcg
    32821 agtaaccgtc tatggagagc atttaacccc ttttcaatta ttaaggcgtg taaaaagcta
    32881 tatctattat cgagaaaaat atcttaagga aaggaggaca atcacatgaa ttttgggcag
    32941 aatttgtaca actggttctt ggataatgca cagtcattgg tattgttagc gattgttgtc
    33001 attggactgt ttttaggttt taaacgagaa ttttcaaaac tcattggctt cttgattatt
    33061 gctctaatcg ccgtcggact tgtgttcaat gcagctggcg tgaaagatgt gttattaaac
    33121 ttatttaacc gaatcattgg tgcttaacag atatttttca tgataaaata ggaagtatat
    33181 attttgttat gaaaggattt atgaatatgt ttacaaatga tcaatttaaa tctcttttaa
    33241 ttaaaacatt agaaagaaag agctcttcaa attattatga aggaggtaac gagattgttt
    33301 ctaaaatgaa aatatcagag gtaactttag atgagactga cgattttact tattataaag
    33361 gatataaaaa gggctggaat acgctttgca catatcttaa aataagagtt ccttttgatg
    33421 atttagactt ttttgaaagt cataaagatt tgattaccca gacagcaagc tccatatatg
    33481 ataaacaagg tgataatgta ttagtggata ctattttagt accacttcca gagaattacg
    33541 aagtaattaa ctttagtcag ttgaaaatta gtgatgtggt ttcacaagct attgaagacg
    33601 ctgagtcttt tatgagtaat ggtgaatatc aacgtgcctt tgacagagtt cacactgctt
    33661 ttcatggata tttaattgaa atactaaaaa aatatgaaat tacagttcct agagacgaaa
    33721 atctatctaa attatatagt aggatacaac aattaattga aaaagaaatt caacctactg
    33781 aacttgctga tatagtgaag acaacgataa ggtcatcaaa tggaatgatt agctctctta
    33841 atgaagcaag aaataggcat tcacttgcac accctaatac aaatattatt ggaaagagag
    33901 aagcaaaact aataataggt atttcaagta cagttacaga ttatattagc ggataccttg
    33961 ataagtagcg tcattaactg attgctactt tttttatgac tatttttata aaaaggatgt
    34021 gataagaatt ggaaatgaaa gtctatgtgg caaatttagg acggtataat gaaggtgaat
    34081 tagtcggagc atggtttact ccaccaattg atgaggaaga aatggcagaa agaatcggtt
    34141 taaatgagga ctacgaagaa tatgcaatac atgattttga gcttcctttt gatgtggacg
    34201 aatacacgcc gattagtgaa atcaaccgtc tatgtgaagc cattcaagaa atcgaaggta
    34261 cacctattta taatgaactc aaagaaattc aaggcatgtg gtttagtagc ttggaagaat
    34321 tactggaaaa caaagaggat attcattgtt atagtgattg tgattcgatg gaagatgtcg
    34381 cccgttatta cgtggaagaa acaggacagc ttggtgaagt cccttccaat ttacaaaatt
    34441 atattgacta ccaatctttg ggacgtgata tggaaataga gggtaattac ctagttacct
    34501 ctcatggggt atttgaatat tgtcagtaag tttaacaatc cgtgttataa ttaaattaat
    34561 aaatagcacg gagaggtata aattatggta aatgtatttg aaaatcttaa actattaaaa
    34621 ctagacatga aagataaagg ctgggtgata gattcctttt actttcgtta caaacagcaa
    34681 aattatattg tacttgttaa gctatttgaa aaagaggaaa aagttccaga atatgctctg
    34741 ttaaaactag aatttttaaa ggaaaacgat ttttcagata tgctagctgt ctatgctaat
    34801 tcggtaaaat tatatacaga cacgaaaaca ataagagaat attttggtat cgaatacagt
    34861 agtaacttgg gagatgtttt atttcaattt agccaaactc ttgctcgatt tattccgaca
    34921 gaagttagtg agaagaaaaa tgaagaccaa aaagaagcca tgtgtttctc attaagtcaa
    34981 agtgattcag aagatcccag aaaaaaatat tgtttttcag ttagaagaaa tcctttaaag
    35041 ggaaatggtg agctgggaaa acgaagtcct tttaatgaca ataaaacgag attgtataga
    35101 cccagcttat atgaacgatt agggtcagat accaatttaa gtttcagata ttcaatgaat
    35161 ccagatgatg aagaaactga tgaaggtatt attgcaaaat ggacaaaaaa taaaattgaa
    35221 tagttaaagc attgaaagag caatcaccta ttttgattgt tctttttcta tttttacgga
    35281 aaggacgtga agacatgaaa aaaataagaa gttacaccag tatttggtca gtagaaaaag
    35341 tgatttatgc gattaatgat ttacaactcc ctttcccaat tacctttacg caaatgacat
    35401 ggtttatcgt ttctttgttt gtcgtcatac tctttggcag tttaccacca ctttctctga
    35461 ttgacggagc actactcaaa tacttaggga ttcccattgt tctcactggg ttcatgtgcc
    35521 aaaagacctt tgacggaaag aaaccctatg gatttctaaa gtctatcatt acctatgctc
    35581 tacgccctaa agtaacctac gctggcaaac aaatacgatt aaaaaaagaa acaatgaatg
    35641 aatcgattac gattgtaagg agtgaacaac tagatggcat atccgattaa atatatggaa
    35701 gataatctcg tatttaatca tgatggcgaa gtattcgctt attatgaatt aattccctat
    35761 aattacagtt ttttaagttc cgatgaaaaa attcaagttc atgataattt ctgtcaattg
    35821 attgcccaaa atcgtgatgg caagattcat gctctgcaac tagcaaccga atcaagtatt
    35881 cgagccacac aggaacaatc gaaaaaagaa atcactggac gattaaaaga gatagcttac
    35941 gaaaaaatag atgaacagac cgaagcgtta atttctatga ttggtgacaa tcaagtagac
    36001 tatcgctttt ttattggttt taaactctac ctcaatgaac aggaagtatc cgtaaaaaat
    36061 gttgggaaag aaattcaaaa agcatttgcg gaattctttc atggtgtgaa tcacaaatta
    36121 atgggcgatt ttgtttctat gcctatggcg gaaatcgaac gcttttctaa gatggaacag
    36181 ttgttagaaa gtaaattatc cagacgcttt cagattcgta aattggagaa agatgatttt
    36241 ggttatatta ttgaacatct ttacgggcaa acaggtgtcc cttttgatga ctacgactac
    36301 catttaccga aacgatacga agataaaaaa gtgttggtaa agaaatacga tattctcaaa
    36361 cccactcgct gtttaatcga agaaaatcaa cgctatttac ggattgaaca ggaagatacc
    36421 actacttatg tggcgtattt tacaattaat agtattgttg gtgaattgga tttccctaat
    36481 agtgaaattt tttattacca gcaacagcag tttactttcc ccgttgatac cagtatgaat
    36541 gttgaaattg tcgccaataa aaaagcctta tctaccgttc gcaataaaaa gaaagaactg
    36601 aaagatttag atagccacgc atgggaaagc gacaacgaaa ccagtactaa tgtggtggac
    36661 gctttagaat ctgtcgatga attagaaagt aatttagacc aaaccaaaga atctatgtac
    36721 aaactaagct atgtggtacg ggtatcagca aagaatttgg acgagctgaa acaacgttgt
    36781 gatgaagtaa aagattttta tgatgatctg tctatcaaat tagtacgacc ttttggcgat
    36841 atgctaggct tgcataatga atttattccc tcaagcaagc gatatatcaa tgactatatt
    36901 caatatgtca cgtcggattt tttggctggt cttggtttcg gtgctacaca aatgattggt
    36961 gaaaattctg gtatttacgt cggttacaat ctcgatacag gcagaaatat ttatctccaa
    37021 cctagccttg caagtcaagg aattaaaggc tctgtaacaa atgcattagc gtccgctttt
    37081 attggttcat taggtggcgg aaaatcattc tctaacaata tgctggtcta ttattcagta
    37141 ctctttggcg gacaggcaac cattgttgac ccaaaagctg aacgtggtga atggaaagaa
    37201 accttacctg atattgccca tgaaatcaat attgttaatc tcacgagtga gaacgacaat
    37261 aaagggttac ttgaccctta tgtgatttta aaactcccga aagattcaga aagccttgcc
    37321 attgatattc tcacgttctt aacaggtatt tccagccgag atagcgagaa gtttcctgtc
    37381 ctgcgtaaag ctattcggag tgtaactcaa agcaaacaac gagggttgtt attggtgatt
    37441 gatgaattgc gaaaagaaga tacgcccatc agcaatagta ttgctgacca tatcgaaagc
    37501 tttgtcgatt atgattttgc ccatctgtta ttcagtgatg gcagtgtcac ccaatccatt
    37561 agcttggaaa aacaactcaa tattattcaa gtggcagatt tggtcttacc cgattccaat
    37621 agctcttttg aagaatacac taccatggaa ttattaagtg tggcaatgct gattgtcatt
    37681 agtacctttg ctttagactt tatccactct gaccgttcca tttttaaaat tgttgacctt
    37741 gatgaggctt ggagtttttt acaagtggca caaggaaaag ctctatctat gcgattggtt
    37801 cgagcaggac gtgccatgaa tgcaggggtc tatttcgtta ctcaaaacgc ggacgattta
    37861 ctagatgaaa agatgaagaa taatattggc ttgaaatttg cctttcgttc cactgaccta
    37921 gtggaaatca aaaagacctt agaatttttt ggtgtggaca aggaagatga aaacaatcag
    37981 aaacgcttac gtgaattaga aaatggacaa tgtttagttt cagacatcta tggtcgtgta
    38041 ggggttatgc agtttcaccc gatttttgaa gagttgcttc atgcctttga taccagaccg
    38101 cctgtgcgaa atgaggtgga agaatgacac aaaaacaaaa gaaatttctt cactatagtt
    38161 ggataacgct cctttgtgtg ggcgttattt tacttttatt aggcacgctg ggcaatgcag
    38221 tacaagcgac aggcttagtt gatgaaacca ttgatacaag caatgaatac tccaagtatg
    38281 gtttgaatca atatcagctt gattattatg tggacaatag ctggggctgg cttccatgga
    38341 attggagtga tggcatagga caatcggtga tgtatggatt gtatgccatt actaatttta
    38401 tttggacaat ttctctttat ctatcgaacg ccacaggtta tttggtgaaa gaagcctatg
    38461 ccttagattt cattagtcaa acatcccaag caattggcga aaacatgcag acgattgcag
    38521 gcattacacc gaatggcttt tccagttcag ggttttatgt cggtttcctc cttcttttta
    38581 ttctcattgt tggaatctat gttgcctata caggattggt taagcgagaa acgacgaaag
    38641 tgatacgagc tgttttaaac tttgtcgtgg tgtttattct atcggcttcc tttattgcgt
    38701 atgcacccga ttatattgcg aaaatcaatg atttttccaa agatgtcagt accgccagtc
    38761 tgaatattgg gacaaaaatt gttcttccta acacggatag tcaagggaaa gacagtgtgg
    38821 acatgatacg tgacagctta ttctctgttc aagtaaagca accatggcta ttgcttcaat
    38881 atggctccac tgatgtagaa gcgttagggg aagaacgtgt ggaaagttta ttgtctacca
    38941 gtccagatac aaacaatggt gaagatagag aaaatgtagt gattgaagaa attgaagatc
    39001 atgacaatac ctatttaacc ctaccgaaga cgatttccag attaggaaca gtcttctttt
    39061 tgtttgtatt caatattggt atctcgattt ttgttttcct tctaacagga atcatgattt
    39121 tttcgcaagt gctatttatt attttcgcta tgtttttgcc aatcagtttc ctattaagta
    39181 tgttgccgag ttttgatggc atgggcaggc atgccattac caagttattt aatgtgatta
    39241 tgacaagggc tggtattacc ttgattatta ccgttgcctt tagtatttcc actatgcttt
    39301 attcgctgtc agcaagttct ccctttttca tgattatgtt tttacaaatt gtgacctttg
    39361 cagggattta ttttaaactg ggcgatttaa tgagcctatt cactttacaa agcaacgact
    39421 ctcaaaacat gggcagacaa gtgtttcgca gacctcgtca aatgatgaat cgacaaacca
    39481 gaagatttca acgcagtatg agtaagattt ttcgtggaaa agcagggtca acgaagcaaa
    39541 agacaatgac aacaaagcag aatcaaacca ataaagctaa ccatactcgt caaaatgaac
    39601 ggactacacc aaagaaagaa acagcacttt ccaatgtgaa aaagcaacaa ggcggtatcg
    39661 ggcaacgatt aggtgaaaag acagcccatg tattggacac gaaaaatcgt ctagtcaata
    39721 aaagaaagca ggcaaaacaa cagatacagg cgacaccaac ccatgtcaaa tatgcccttc
    39781 atcaaggaaa agagaatgtc actcataatg taacagactt taaacaatct atggcagata
    39841 ccaaagctca acgcaaacaa cagcgtatga accagcagga gcaaaaacga aagaacattg
    39901 ctcaaaagcg tttggagctg gataaagcaa aagaaaaaag acgaaaagaa aacccatcat
    39961 ctcctgttca gaataaccag cagcatgttc gcaagcgtca gcaaaccgtc acgcctgttt
    40021 tgtcacaaaa cactaaacgt gaaaatcatc agcgaccatt gaaaaaagag caaaaacaaa
    40081 agacaccacc gaagcaattc aatcagagga agctaaataa aagaacggat aagcgaggac
    40141 aacaaacatg aagctaaaaa taacagtgat ttgtagtgtg gtcttctctc tcttcttttt
    40201 tctcttatta ttcctagtca ttttctttgc agatgatacg gacagtggcg aaaacaacaa
    40261 ggattcttct attccacaag gcggtgtgac cgtttcacca gaagtgttgg cacaccgtcc
    40321 gttgattgaa aaatatggta aggaatatgg gattgaagac tatgtttctt atatcttagc
    40381 cattatgcaa gtggaatcgg gcggtacagc agaagatgtt atgcaaagtt ccgaatcttt
    40441 gggtttaccg cccaacagtt tgagtaccga agagtccatc aaacaagggg ttaaatattt
    40501 cagtgaatta ttaaccagtg cagaacaaca aggagtggat atagattctg ttatccaatc
    40561 ttataattat ggcggtggct ttttaaatta tgtgaggagt catgggaaaa aatataccta
    40621 tgaattagcc gaacagtttt ctaaagagaa gtcgggtggt caaaaagcag actatcccaa
    40681 tcctatagcc atacctgtga atggtggttg gcgatataac tatggaaatc agttttatgt
    40741 tcagttggtt tcacaatacc taaccgacac ttccccaaca gaatttgatg atgaaaccgt
    40801 gcaagtcatt atggacgaag ccttgaaata cgaagggttt ccctatgtct ttggaggggc
    40861 gtcgcctacc acctcttttg attgtagtgg cttaatacaa tgggtgtatg acaaagctgg
    40921 catttctctc cctcgtgtcg cccaagatca gtatgacgct acgcaagaaa tctctatgga
    40981 agaagcccaa gcaggcgacc ttatcttctt tcattcaacc tataatgcag gcacatatgt
    41041 gactcatgtg gcgatatatt tagagggaaa tcgcttttat catgcaggcg acccaatcgg
    41101 ttatggcgac ttaagcagtc gttattggca agaccatctg attggggcta gacgtgttat
    41161 tcataactaa gaaaggaatg aatcaaatga ttataaaaat cgagcgaaaa gaaaagaagg
    41221 agaaaccaaa gaaatctaaa aaaccgagca agcaaagaaa agttccgatg attaaagttg
    41281 gcacgcataa gaaattaacc tttgttttat gggttttatt gattggtagt gtcggatttg
    41341 gaatctataa aaattttact gccattgaca ctcatactgt ccgtgaaaca gaaatcatta
    41401 aacagcagat tgtcgatacc aaccaagtag aaagttttgt gaaatccttt gcgaaagact
    41461 acttttcgtg gcaacaatcc caagaagcca ttgataaacg aaatgaaaaa ttgacccatt
    41521 atttgacgga agaattgcag gtattaaatg aagaaatgat tcgtaaggat attcctacta
    41581 gttctagtgt gaacgatata caggtgtggc aggtttctca agtgaatgag aacacctttg
    41641 aggtactgtt ttctgttgaa caagttatta ccgaagacaa agacaaggaa actatatcgt
    41701 ctagctttca tgtggttgtc catatagatg aatcagataa tatggttatt atcaaaaatc
    41761 cgacaatgag taagaaacct caaaaatcgg actaccaacc caaacagcta gaaagtgacc
    41821 atacagtaga tacagaaacg atggacgaaa tcactagctt tttagaaacg ttcttccagc
    41881 tctatcctac cgccacagaa aaagaattga cttactatgt aagtaatcat gtgttaccta
    41941 tgattaacaa ggaatatgtt ttcgaggaat tggtgaatcc tatctttacc agaaaagata
    42001 accaagtaat cgtgaatgtc gctgtgaaat atttagatca agaaacaaag gcaacccaaa
    42061 tctcgcaatt tgagttgata ttagaaaaac aagataattg gaagattgta aaataacaaa
    42121 tattggtaca tgattacaga tactttgtaa tcatgtactc tttttgataa aaaattggag
    42181 attcctttac aaatatgctc ttacgtgcta ttatttaagt gactatttaa aaggagttaa
    42241 taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagcggga acaaataatt
    42301 agatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat
    42361 gtgattctaa agtatccaga gaatatctgt atgctttgta tacctatggt tatgcataaa
    42421 aatcccagtg ataaaagtat ttatcactgg gatttttatg cccttttggg tttttgaatg
    42481 gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa
    42541 actaccttaa cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg
    42601 gacaaaggta caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt
    42661 cagacaggaa taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca
    42721 ggacatatgg atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt
    42781 ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt
    42841 aggaaaatgg ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat
    42901 ttatcaacgg tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag
    42961 aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat
    43021 acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa
    43081 gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt
    43141 tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat
    43201 aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt
    43261 gaatatacaa aaaaaagaca acgtcttgca tattaagtag ttcagttttg gagtacaaaa
    43321 agtgaaaatg taaacctaaa tgagtatcaa attgcaactg aatttgatac tcgtttttta
    43381 ttgttaaatc aacgtttgtt gttaatttac accacaaaat gtaagttaaa atgtatataa
    43441 aaatgcgaac gtgaaataat tattgtagga atgcaaattg gtgtgttaaa atggttacaa
    43501 tttactaagc aaaaaggggt gcattttcct atgcaatttc ttgagccaaa aaataagaac
    43561 gctaagtctg tggattggga aatatcagag caagtaaggg tgatagttaa gcaatacgct
    43621 gaatatgcag agcgtactga aagcgaagca gtggatgaat tcttattgaa cattttagac
    43681 gataagaagt ttattgaatg gattgccaac aagcgaagta ataagcggat tgttgaaaaa
    43741 atgggtatca aagatagagt gggttgatag acaatgacca agcttaaaag attagccacg
    43801 aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa acgaagaatt aaaagatagg
    43861 gctaaacaat atgatttatt aactccaaaa cgatttgcaa caaggtataa caaaatgttg
    43921 ttcttgccaa cgtcttttaa atggaacggt tctgaatatc ccattcaata taattactgc
    43981 atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca aattcaaaga tgtaaaaggg
    44041 aaaccaagtc gatataaaat gactggtagt tccaaagata aaggtcataa aggtatgtac
    44101 tgcaatgaca atcctattgg cagaggtgta tcgcaaaact gtacagtaac ccctttatct
    44161 aattggtctg ttgttgaaga aattaaacgc cttatagaaa tcaatagcat ccaagatgtt
    44221 gagccagatt atcaatttca taaagaaggc tgctcggaag aagaatcaac cccattcaat
    44281 gagccgaagc aattttacaa aagggggaaa agtaggggca aatctcaacg ctatcaatgt
    44341 aaggcgtgta agaagttcac taatgttcta ccaaaaagag aagaaaccac tacttatcac
    44401 caacaaaaga acaccattct tccaatgttc gctaagatgg ttgttggaag ggtttcagtc
    44461 agtcgtactt gtgatatttt aggaattgga gttggaacct actatcataa gctggagtgg
    44521 ctgtataggc gttgtttgga gtttttagaa cgatatgaga cacaacccct tcaaacaaag
    44581 aaatttaacg agatgtggct taatacggac aagatgcact actacctaaa taacgtccgt
    44641 aagaaaggac aaggttcaaa gaaatatacg gggtttgaag accttaatat gcaaacgtat
    44701 atagttgtat ctgctgaggt cttatcaaga tatgtgttcc gttctgatgt tgcctatgat
    44761 tggaatattt caatggatga attaaatgaa gatacaagaa aattcaaaga agaccatctc
    44821 aatactttta gtcgtaaaaa tgataggtta gattggtctt attatccaca agaaccatcc
    44881 gctaacgata gcgaaaatag gaatgcttat cttcacgaat tagggaaaat tactaatagg
    44941 agtagatttg tcgatggttt aaatgttgat gctccttata ctacaactgc tcactattgg
    45001 ttaattaaac aaatggttaa tgccgacgaa tggcgaatga ttagtgatga tgacttctct
    45061 attagaaacg ccttttatag agtattcacg aaagaattaa ggttgagcga tgcacatcat
    45121 tttatttgcc aagttaacaa gacgaaaagc agaaaacaat gcttaaaaga attcggacaa
    45181 gcaaaagcag aactgctcga ttggggagat atcagaggat tcaaaacaaa gtttctacgg
    45241 actttggcat ctcattatct aactgaatta ctaacaagtc atcaattcca cgaagaggct
    45301 attagtaaag atggagaaag atatcgcaaa tatgccgata accctatcaa acatccatta
    45361 gccactaagg ataaaggatt ttattcagtt gattgtagaa cggatttatc tgctctggaa
    45421 ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc attcgaccaa tagctttatt
    45481 caacagataa gacgttatat atcatctctt gaaagaccat taacaacagc tcgtggtgat
    45541 aagaagagct atatttacgc taacttcaat ccgaagtatg ctcaatttgc gattacaatt
    45601 ctaagaactt actacaactt ttgtcgccct tttaaatcag cggataaaaa ggttttgaca
    45661 cctgctcaaa gattagggat tacagataaa caatttgatt ggaaagatat aatttatttc
    45721 aaatgacaaa aagagcttgg agtaatccca agctcttgtg ctttatcttt actgttcttc
    45781 ttcaattaac gcacccgtta actcaataag ttttttcttt catatacatg aaaatagtag
    45841 ttatacggat ttttatcatt ctttatccct ttttgggcaa atacctcatt ccattcctca
    45901 taattcactt ctggaaaaaa agtatctcct tcgaattcat gatgtatttt tgtgatgtac
    45961 attttctcaa cataagggaa aaacaaatta taaatctgtt ctcctccgaa aataaaaatt
    46021 tcttcttcgt ttttacataa ctcaaaaaca tcttctattg aatgaacaat ttcacaacca
    46081 ttaaaggtaa accccttatc tctcgtcaga ataatatttc ttctgtcagg taaggctctt
    46141 ccgattgatt caaggttctt cctacctaat attatcggat gtccctttgt agtattttta
    46201 acatattccc agtccttggg aatcctccaa ggaatgtcat tctctttgcc aatcactcta
    46261 ttcttatcca tcgcagcaat caaagaaact ttcatttata acacagcctt tttaactaat
    46321 ttaccatcta atttcttatt tctatcaaaa aaccttttct cattatcctg cccgttagtt
    46381 gaataagcaa ttttttctat aagttatatt atcataatac cacaaaatca caaattttca
    46441 ttcaattatc attttcaatt gcattttata aatcattttg tatgtaattt ggtatgtaag
    46501 ttaaaatgaa aaaagtagtc cattttgaag tacattttgt cattttgtac tccaaaactg
    46561 aactacttac ttgcatatat acgcctttat agtggagtac tacatttacg agattcggtt
    46621 agagtatcag aaaaagaaaa aataaaagtt acagaaatgt atacttcaat aaatggtgaa
    46681 ttatgtaaga ttgatagagc ttattctgga gaaattgtta ttttgcaaaa tgagtttttg
    46741 aagttaaata gtgttcttgg agatacaaaa ctattgccac agagaaaaaa gattgaaaat
    46801 ccgcaccctc tactacaaac aactgttgaa ccgagtaaac ctgaacagag agaaatgttg
    46861 cttgatgccc ttttggaaat ctcagatagt gatccgcttc tacgatatta cgtggattct
    46921 acgacacatg aaattatact ttctttctta gggaaagtac aaatggaagt gattagtgca
    46981 ctgttgcaag aaaagtatca tgtggagata gaactaaaag agcctacagt catttatatg
    47041 gagagaccgt taaaaaatgc agaatatacc attcacatcg aagtgccgcc aaatcctttc
    47101 tgggcttcca ttggtttatc tgtatcaccg cttccgttgg gaagtggaat gcagtatgag
    47161 agctcggttt ctcttggata cttaaatcaa tcatttcaaa atgcagttat ggaagggata
    47221 cgctatggtt gcgaacaagg attatatggt tggaatgtga cggattgtaa aatctgtttt
    47281 aagtacggtt tatactatag ccctgttagt actccagcag attttcggat gcttactcct
    47341 attgtactgg agcaagcctt tagaaaagct ggaacagaat tgttagagcc atatcttagt
    47401 tttaaagttt atgcaccaca ggaatatctt tcacgggcat ataacgatgc tcccaaatat
    47461 tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg tcattattat tggagaaatt
    47521 cctgctcgat gtattcaaga ttatcgcaat gatttaactt tttttacaaa tgggcttagt
    47581 gtttgtttag cagagctaaa aggatatcag gttaccactg gcgaacctgt ttgccagacc
    47641 cgtcgtctaa atagtcggat agataaagta agatatatgt tcaataaaat aacttagtgc
    47701 gttttatgtt gttatataaa tatggtttct tattaaataa gatgaaatat tctttaatat
    47761 agatttgaat taaagtggaa aggaggagat tgttattata aactacaagt ggatattgtg
    47821 tcctatttgt ggaaataaaa caagactacg aatacgagtg gatactatac ttaaaaattt
    47881 ccctttatac tgccccaaat gtaagaacga aactttaatt aatgttcaaa aaatgaatat
    47941 aataacaatc aaagagccag acgccaagac gcagagccga taatttgaga aatgaaactc
    48001 tcatcttatc ggctcttttt gtttatctga attttactga ctagccttca atatttcttg
    48061 cttcattgat accttttgct agtgcttcca ttaaggatag ttctttgtct gtaaagctat
    48121 ccatgtattt ttctacctgt aaccttcgag tgcttttaat caaattatct gtaggtaaga
    48181 aaaattcatc aatagataca tggagtaatg atacaagatc atacagcact tgtgtacttg
    48241 ggtgttgtcc tttgttttct atatttgtta aataacgtgg gtcaatttca atcattgttc
    48301 ccacttgttc acgagtgaga ccccgtttca ttcgagcctc tttaatcgct aaaccaacgg
    48361 ctctaaaatc gtatttatct tcttttttac gcatactaaa tcacctctat acattttact
    48421 gttcctatat aaccataaac aggtatagaa aaacatgtta tatagtttat gagttcctat
    48481 agaacactgt ggaatttatt aatgcgatga atccaatttc aaatagaaag gtcgctgatt
    48541 ttatttacct atacaacttt ataaaattta gtcatactcc tagaaagtag ataaaattgg
    48601 cgacttaatg gagggaaaag tcgccatgag aaagataatc tgtcatctag ttctcttaat
    48661 taagttctta tcaaaaaaac ttatccacct gtactggata gatatgacca tcaatgtggt
    48721 tttgcagatt acatagatag aaaaatcaaa gcatatgaac agagaaaaca atctgtttat
    48781 gtgctttttt ggtcacttaa taaatatctt ttaggatttc tcttcattta tgcaacaaat
    48841 cccccgttcg tgtcggggtt tagtatgaag ggagataaag aagcacatgc cactcccttt
    48901 cacgacagaa agggggtgaa aagcatgaaa ccatcttctt ttcaagaaac catagaaaat
    48961 cagtttgact atatctgcaa acaagttatt gaagatgaac gaaaagatta cttcaaacat
    49021 ctagacagac tatcagcaaa agaagtctcg ttttctgata tagataacta ccttgtcaac
    49081 tctttttcta ctgttgatac gtacgtcact gatttccaac tatttaaatt atttggatta
    49141 agaatcaatg ttgaaagtga tttattaagt gaagccctgc gtcatctttc agagaaaaaa
    49201 cggaacatta ttttacttca ttactttatg gatatgagtg atgtggagat tgcggagtta
    49261 ctaaatttga atcgttcgac tgtttatcgt catcgaataa gtggactagc catgattaaa
    49321 gaattcatga aggagtgtaa agaatgaaaa cagcttatcc aagggttccc ttccccctca
    49381 ttgtaaaggc gacagatggc gacgtggaag caattaatca gattgttaaa cattatcgag
    49441 ggtatacctc aaaacgttca ttacgtcgca tgacagacga atatggaaat tctcatatgg
    49501 ttattgatga aaccctacgt ggcagaatgg aaacgaaact catctctaag attttatcgt
    49561 tcgaaattaa atagtcccat tttttcgtgg aagcgtgtcg aatcgtatcc acgcttcccg
    49621 atttgggagg tttgttattc catcaaagca tattgtgtgt tcagtaggtt ttgataggct
    49681 aacaagccaa acgttctttg acaaccaaat aaaggcaatc gaatacgttt cggtaagaaa
    49741 agagctgatg aactaacacg ccatgaccta caaaattagt gagcgattca tgttagtaat
    49801 tcgaaagtaa tctttagcag gattgctgcc atgactttct tatcgtgata atgatactcc
    49861 cgtatagcca tagtccgagc gttagaagcg tcacaggcaa tgggtacggc tacatgagaa
    49921 ccatgcagaa gtgaaattcc tgtgagcttt gctagagctg ttcgattgct aagatattct
    49981 tctcttgtca gtgtattatt ataaaactat ttgatataat tataggtgaa tattggagtt
    50041 ttcctagcct ataattggga aaggagtaat aatgataacg gtcacaaaaa aggatttagt
    50101 tgagctagga tatggaactt ctttttcagc agacatcata aaaaaagcaa aagaattgat
    50161 gattacaaaa gggcatatct actatcaatc tcgaaagcta gatagagtcc ctcgtgaagc
    50221 tgttgaagaa attctaggaa ttcggttttc aaatgaaaac aggtctgagt agtttttgca
    50281 cagtaaggag taaaaactat ggttaaagac ccaataaaaa aagcaaagaa tggaacctat
    50341 tattttcgtg caaatctagg ttatgattct aaagggaaaa aaattcaaaa atatcgtagt
    50401 ggattcacta ctaaaaaaga agcaagagaa gaatattcga aactgctatt gatgaaacca
    50461 gaagaattaa gtgagaataa ggataaaatg acgtttaaac attatatctt tgaaattttc
    50521 cttccatggt ataagacaca ggtaaagctc agaacatatg aaaatcgctt accaactatt
    50581 aagaagcatt tctcttattt tgataaaatg gctgtttccg atattgagcc tattgatgtg
    50641 caaaattggc aactaaaatt atcaaagaag tgtaagtctt cttatgtaag agctgttcaa
    50701 ggactatttt cagttgctat ggatcgagca attgtccttg ggataaccac cacaaatcca
    50761 tctaagatta ttggcaatgt gaaaaagcaa aagtctaaaa ttgaattttg gacaaaagaa
    50821 gaatttgaaa aagtaatctc tctaatttac aaagaagatt actatcaaca ctttttattt
    50881 atctctctgt ggtttttatt tatgactgga atgcgaattg gtgaagctac tgccattcaa
    50941 tgggaagata ttgattttga ctcaggagta ctaaccatca acaaaacact ctattacaag
    51001 aatcaatcga attatagatt tgttgaacca aaaacaaagg cgagcgttcg tcatattgta
    51061 ttagataaat gcacattaac ttatttatcc gaatggaagg aagtacaaca aaatctaatt
    51121 cagacagact ttgtaatgag ctataacggt attcctaccc aaaaacatac actatcatat
    51181 gctattgaac gctatgctaa gctggctggt atccatcgaa tcagaatcca tgggttacgg
    51241 cattctcatg cttccttgtt aatcagtatg ggagaaaatc cacttatcat taaagatcgt
    51301 ctaggacacg aagatattga gacgacttta ggaacgtatg gacatcttta tcctaacagt
    51361 aatttcgagg tagctaacaa attaaatgga gccatttcct ttaaagaagc aaaacaaaat
    51421 tatgatactt caccaaagaa tcaatttact gtagactact tgcgaaacaa agaaatgaaa
    51481 aaagtgcaat aaaagtgcag taaaaagata agaagagcca gaaacccttg gtataagagg
//
LOCUS       DACIWG010000019        48861 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.019, whole genome shotgun sequence.
ACCESSION   DACIWG010000019 DACIWG010000000
VERSION     DACIWG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.019"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IYF54_001084"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IYF54_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ9831427.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IYF54_001085"
     CDS             complement(2774..3259)
                     /locus_tag="IYF54_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ9831428.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IYF54_001086"
     CDS             3739..4380
                     /locus_tag="IYF54_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9831429.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IYF54_001087"
     CDS             4399..5034
                     /locus_tag="IYF54_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831430.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IYF54_001088"
     CDS             5052..5867
                     /locus_tag="IYF54_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9831431.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IYF54_001089"
     CDS             6186..7622
                     /locus_tag="IYF54_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9831432.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IYF54_001090"
     CDS             7811..10258
                     /locus_tag="IYF54_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ9831433.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IYF54_001091"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IYF54_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ9831434.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IYF54_001092"
     CDS             complement(11748..12851)
                     /locus_tag="IYF54_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9831435.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13404..13952
                     /gene="aac(6')"
                     /locus_tag="IYF54_001093"
     CDS             13404..13952
                     /gene="aac(6')"
                     /locus_tag="IYF54_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ9831436.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(14512..16404)
                     /locus_tag="IYF54_001094"
     CDS             complement(14512..16404)
                     /locus_tag="IYF54_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831437.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(16401..18128)
                     /locus_tag="IYF54_001095"
     CDS             complement(16401..18128)
                     /locus_tag="IYF54_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831438.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(18144..18794)
                     /locus_tag="IYF54_001096"
     CDS             complement(18144..18794)
                     /locus_tag="IYF54_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831439.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18998..19294
                     /locus_tag="IYF54_001097"
     CDS             18998..19294
                     /locus_tag="IYF54_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ9831440.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            19486..20106
                     /locus_tag="IYF54_001098"
     CDS             19486..20106
                     /locus_tag="IYF54_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ9831441.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(20198..21364)
                     /locus_tag="IYF54_001099"
     CDS             complement(20198..21364)
                     /locus_tag="IYF54_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9831442.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21376..21729)
                     /locus_tag="IYF54_001100"
     CDS             complement(21376..21729)
                     /locus_tag="IYF54_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ9831443.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            21857..22879
                     /locus_tag="IYF54_001101"
     CDS             21857..22879
                     /locus_tag="IYF54_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ9831444.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(23318..23752)
                     /locus_tag="IYF54_001102"
     CDS             complement(23318..23752)
                     /locus_tag="IYF54_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831445.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(23862..25721)
                     /locus_tag="IYF54_001103"
     CDS             complement(23862..25721)
                     /locus_tag="IYF54_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9831446.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(25839..26999)
                     /locus_tag="IYF54_001104"
     CDS             complement(25839..26999)
                     /locus_tag="IYF54_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ9831447.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(27306..28730)
                     /gene="araA"
                     /locus_tag="IYF54_001105"
     CDS             complement(27306..28730)
                     /gene="araA"
                     /locus_tag="IYF54_001105"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ9831448.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(28746..29444)
                     /locus_tag="IYF54_001106"
     CDS             complement(28746..29444)
                     /locus_tag="IYF54_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ9831449.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(29401..31062)
                     /locus_tag="IYF54_001107"
     CDS             complement(29401..31062)
                     /locus_tag="IYF54_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ9831450.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            31280..32680
                     /locus_tag="IYF54_001108"
     CDS             31280..32680
                     /locus_tag="IYF54_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ9831451.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(32932..34011)
                     /locus_tag="IYF54_001109"
     CDS             complement(32932..34011)
                     /locus_tag="IYF54_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9831452.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(34097..35056)
                     /locus_tag="IYF54_001110"
     CDS             complement(34097..35056)
                     /locus_tag="IYF54_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ9831453.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(35068..36591)
                     /locus_tag="IYF54_001111"
     CDS             complement(35068..36591)
                     /locus_tag="IYF54_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ9831454.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(36601..37164)
                     /locus_tag="IYF54_001112"
     CDS             complement(36601..37164)
                     /locus_tag="IYF54_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ9831455.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(37170..38033)
                     /locus_tag="IYF54_001113"
     CDS             complement(37170..38033)
                     /locus_tag="IYF54_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9831456.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(38047..38943)
                     /locus_tag="IYF54_001114"
     CDS             complement(38047..38943)
                     /locus_tag="IYF54_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9831457.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(39118..40389)
                     /locus_tag="IYF54_001115"
     CDS             complement(39118..40389)
                     /locus_tag="IYF54_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9831458.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(40379..41896)
                     /locus_tag="IYF54_001116"
     CDS             complement(40379..41896)
                     /locus_tag="IYF54_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ9831459.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            42094..42984
                     /locus_tag="IYF54_001117"
     CDS             42094..42984
                     /locus_tag="IYF54_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ9831460.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(43057..43881)
                     /gene="nadE"
                     /locus_tag="IYF54_001118"
     CDS             complement(43057..43881)
                     /gene="nadE"
                     /locus_tag="IYF54_001118"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ9831461.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(43893..45365)
                     /locus_tag="IYF54_001119"
     CDS             complement(43893..45365)
                     /locus_tag="IYF54_001119"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ9831462.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(46002..46364)
                     /locus_tag="IYF54_001120"
     CDS             complement(46002..46364)
                     /locus_tag="IYF54_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ9831463.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            46587..47339
                     /locus_tag="IYF54_001121"
     CDS             46587..47339
                     /locus_tag="IYF54_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9831464.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(47479..48402)
                     /locus_tag="IYF54_001122"
     CDS             complement(47479..48402)
                     /locus_tag="IYF54_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9831465.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(48417..48689)
                     /locus_tag="IYF54_001123"
     CDS             complement(48417..48689)
                     /locus_tag="IYF54_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831466.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        15113 a         9541 c         8971 g        15236 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    13141 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13201 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13261 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13321 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13381 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13441 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13501 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13561 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13621 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13681 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13741 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13801 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13861 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13921 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13981 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    14041 cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct
    14101 tagtttcgag tagtaagaaa aattttgtga aaatggcttt ctacattttt gcataaattt
    14161 ggcttaatac cgagaaactg gcttttgaac accgtttacc aagaggttgt gagcctgccg
    14221 tttaactccg agtcacaagg agcaatttca aaaaacagct tctcaggttt gttgactatt
    14281 gtgacttgtg accgaggagt tggcaggaga acaccgttta aggaagaggt tgtaaaatca
    14341 gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat attttgccgt
    14401 tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa aaagggactg
    14461 tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat tctatgaagc
    14521 ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt tttccatcaa
    14581 tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa tgttgtctgc
    14641 atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt ccaataggcg
    14701 gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg ttgcttcatc
    14761 aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta gctgacgctg
    14821 cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt ctggcaattt
    14881 acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt cgtctgttgc
    14941 atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc aagtatcttg
    15001 cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg tatctacacc
    15061 atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt tgatcagtgt
    15121 cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt tgacatccaa
    15181 gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat tttcaaaacg
    15241 aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg acatctcttc
    15301 ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga tagtgttcat
    15361 caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca agaacgcttg
    15421 aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta cagcgacgaa
    15481 cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg aaataaattg
    15541 agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt cctggtcttt
    15601 ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt ctacttggtt
    15661 attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt tcggcgctac
    15721 gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg tcaattgcca
    15781 gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag ttacaaattg
    15841 agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg ctcgagacat
    15901 gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca ttttggcttc
    15961 caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa taaattgctg
    16021 taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa tgatctgcgc
    16081 gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca tctgttgagc
    16141 gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct ttggtgtttg
    16201 gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg caaccatacg
    16261 tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag gcttttcacc
    16321 ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg gtccatgaga
    16381 gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga atgaacgatt
    16441 tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc aaccagtttt
    16501 ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg ttgagcaact
    16561 aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt ggcatccgtt
    16621 ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt tttgactaat
    16681 gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc tccttgttcc
    16741 acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg tgcgatttct
    16801 aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat attgtctcgg
    16861 atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc tcgcaaatta
    16921 tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga ttcgatgtca
    16981 tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc ccctatgatt
    17041 gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag tttttctgct
    17101 ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc agcaaaagaa
    17161 actgttttag gattttcagg gtcatggata ccgatttttt catccaacac ttcattaata
    17221 cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag catcataaag
    17281 ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac ttccaaagtc
    17341 atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc gctcatgatc
    17401 aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc cgtatttgta
    17461 taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc gaatgctcga
    17521 atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc agttttcttt
    17581 tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa aataatcatt
    17641 actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata agcaagtatg
    17701 ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat ttgtgtaacc
    17761 atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt tccatattta
    17821 tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg caattgctta
    17881 cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc taagatactg
    17941 atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac atctccttgc
    18001 gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc tgccagtact
    18061 tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg ttttacgatt
    18121 tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca agaaagtttc
    18181 aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga aaataagcga
    18241 tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag taattccaat
    18301 tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa ctcttttgca
    18361 tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt atgaggagcg
    18421 acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa agaagcattc
    18481 tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc tttgaataga
    18541 tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg tctcattgtc
    18601 tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa actgcctcta
    18661 gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg tactctagaa
    18721 aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa atggaaaaat
    18781 gtctgtgtcg gcatatggat tctcctttct ttcaagatga cacattgtca tatgacgcta
    18841 tgtcattata acacctccta cctgttttgc aagacatttg catagttttt ttcattatca
    18901 tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg agagttgccc
    18961 attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa ttagtgtcca
    19021 agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa gaaatacctt
    19081 agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta tcttgccaag
    19141 acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata caggcaggcg
    19201 atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt atatcattgg
    19261 tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat tcaaaagagg
    19321 ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta gttatttatt
    19381 cttcaacctt atcaatatga cttgatagcc ttttcttttt cgagtaaact gattttgtta
    19441 gtcatagtta taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta
    19501 gtagtcaaag gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct
    19561 tttttaacaa gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat
    19621 cgcgatgata ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct
    19681 ggtgcagaat tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa
    19741 ttgactgatc agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta
    19801 aatatcccaa caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc
    19861 cgctatacag aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa
    19921 tctaatggcg gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta
    19981 aactttgtag gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct
    20041 gaccgtgcag atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca
    20101 ttttaaaaat tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca
    20161 tttgtgcctt ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg
    20221 gattttcttc ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta
    20281 ctttagctgc tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc
    20341 cagaagtcag cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta
    20401 ctaatggaga gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt
    20461 tgatgatcat gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa
    20521 atgatcctaa gtaagggatc aggtttaaaa aacccgcaat aatccccaac gtgacagcat
    20581 actctaaacc aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag
    20641 ttagttgtcc tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg
    20701 tcggtttttg catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt
    20761 ctttcaatag ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta
    20821 ccgcgccgac aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat
    20881 cgcctgccga attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga
    20941 acagcgggtc ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga
    21001 agttactgat gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc
    21061 atgcgattag tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt
    21121 ttcgcttttc aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa
    21181 tgataggcag acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt
    21241 ttgtaaataa aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt
    21301 gattatttaa aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt
    21361 ccataattcc tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct
    21421 atgcggatga agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat
    21481 caatgatacg tgagctactg tctttaaatt cccattggcc atcggaccga cataatcgat
    21541 cgtatatacc tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc
    21601 ttttgatact tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa
    21661 caaaatcagc atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc
    21721 ctgaatcatt gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc
    21781 tttagccgtg tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta
    21841 ggaggaatca gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag
    21901 taaaggaatc tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt
    21961 agctgttgaa gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac
    22021 agttactagt gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt
    22081 gaatgcggta acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca
    22141 attactatac ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga
    22201 tggcgaactg aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa
    22261 ccaagatcat gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg
    22321 tgatcttggt acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct
    22381 tgtaactacc ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa
    22441 tggacaattc gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa
    22501 taaagaaaca ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga
    22561 cggtgaaaat ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc
    22621 taaccgagga cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca
    22681 aaacaagcaa atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag
    22741 caataatttt gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca
    22801 tcctgaaaca ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg
    22861 tgtttatttt gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga
    22921 cagactacgg gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta
    22981 tagcgaagag gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt
    23041 acagaatgta gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac
    23101 ttttccgtct atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc
    23161 gacggaaaag gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt
    23221 atgtcattat cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt
    23281 agataactct agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag
    23341 tcattttttg gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg
    23401 tattgaatag atcttcatct acccctgatt caagatcgat actttggatc atttcttcac
    23461 agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg
    23521 ctggtggacg caaagtactg actttcataa gcattgcccg atattttttt gttccttctt
    23581 caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat
    23641 caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt
    23701 ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt
    23761 tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag
    23821 tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa
    23881 ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg
    23941 tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga
    24001 tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa
    24061 aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg
    24121 gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat
    24181 tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct
    24241 ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc
    24301 gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca
    24361 agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg
    24421 tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg
    24481 agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc
    24541 gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa
    24601 acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat
    24661 tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg
    24721 taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat
    24781 tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga
    24841 tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga
    24901 ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca
    24961 gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct
    25021 ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg
    25081 ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta
    25141 ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt
    25201 ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg
    25261 cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact
    25321 gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa
    25381 tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat
    25441 acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc
    25501 agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa
    25561 gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat
    25621 aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc
    25681 tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc
    25741 tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac
    25801 ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt
    25861 aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc
    25921 tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta
    25981 cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat
    26041 atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt
    26101 gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct
    26161 tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc
    26221 aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta
    26281 gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt
    26341 cctacattga taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat
    26401 ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact
    26461 gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag
    26521 acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca
    26581 agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct
    26641 gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc
    26701 ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca
    26761 cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc
    26821 accccaacac cagctcttga aattgctagc aaatctggtg taagccattc atctttcact
    26881 ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc
    26941 ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata
    27001 aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat
    27061 ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag
    27121 gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata
    27181 tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt
    27241 gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc
    27301 tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga
    27361 tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa
    27421 tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta
    27481 ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta
    27541 ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc
    27601 gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca
    27661 gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct
    27721 agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc
    27781 aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc
    27841 atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta
    27901 gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga
    27961 gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta
    28021 tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt
    28081 acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct
    28141 tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact
    28201 tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt
    28261 tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca
    28321 ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata
    28381 tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt
    28441 tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg
    28501 atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca
    28561 gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt
    28621 tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa
    28681 tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac
    28741 tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta
    28801 tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc
    28861 atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc
    28921 caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg
    28981 cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt
    29041 tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc
    29101 gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga
    29161 gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta
    29221 tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg
    29281 tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt
    29341 tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag
    29401 tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt
    29461 ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat
    29521 aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg
    29581 tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata
    29641 ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac
    29701 tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc
    29761 cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat ccattccgat
    29821 tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg
    29881 gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc
    29941 ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt
    30001 gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc
    30061 ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc
    30121 tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc
    30181 tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtcccttt
    30241 ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg
    30301 gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt
    30361 gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac
    30421 gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt
    30481 tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt
    30541 aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc
    30601 ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta
    30661 ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg
    30721 aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact
    30781 gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc
    30841 catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat
    30901 accattttct agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga
    30961 atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc
    31021 atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc
    31081 ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact
    31141 tttgtacgta catgtcaagc agttatttac aagaatacaa taaaaaacac ttgttttgta
    31201 cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt
    31261 ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt
    31321 ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt
    31381 acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac
    31441 gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa
    31501 gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct
    31561 ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg
    31621 tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc
    31681 tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct
    31741 ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa acgatggctt ggcgcgtgat
    31801 gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga
    31861 gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta
    31921 tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca
    31981 agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt
    32041 cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta
    32101 ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg
    32161 gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga
    32221 caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttctttcat
    32281 cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt
    32341 gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat
    32401 cgtaggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt
    32461 taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc
    32521 tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca
    32581 agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa
    32641 aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg
    32701 tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa
    32761 aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt
    32821 ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa
    32881 cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc
    32941 gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc
    33001 ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt
    33061 tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt
    33121 tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat
    33181 taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa
    33241 ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc
    33301 ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta
    33361 ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat
    33421 caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt
    33481 taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac
    33541 ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc
    33601 taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt
    33661 actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat
    33721 cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt
    33781 caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt
    33841 acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac
    33901 gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc
    33961 acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc
    34021 ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa
    34081 tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt
    34141 gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc
    34201 ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg
    34261 aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata
    34321 ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc
    34381 acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt
    34441 tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt
    34501 catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt
    34561 cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat
    34621 atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt
    34681 ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta
    34741 cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt
    34801 aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg
    34861 gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg
    34921 actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat
    34981 aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat
    35041 aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta cattccaaga
    35101 gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc
    35161 aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa
    35221 tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg
    35281 ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact
    35341 gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg
    35401 tttcaatact tgacctttag catatttcga taagtgttgc atcacataat aagttggatt
    35461 cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga
    35521 ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa
    35581 taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc
    35641 cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt
    35701 tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg
    35761 acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg
    35821 aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc
    35881 tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg
    35941 tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt
    36001 tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg
    36061 accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt
    36121 ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt
    36181 ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac
    36241 atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca
    36301 tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata
    36361 accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata
    36421 agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat
    36481 ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat
    36541 ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc
    36601 ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa
    36661 gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca
    36721 acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg
    36781 taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga
    36841 ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa
    36901 ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa
    36961 atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca
    37021 ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
    37081 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
    37141 aagtttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
    37201 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
    37261 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
    37321 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
    37381 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
    37441 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
    37501 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
    37561 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
    37621 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
    37681 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
    37741 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
    37801 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
    37861 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
    37921 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
    37981 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
    38041 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
    38101 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
    38161 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
    38221 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
    38281 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
    38341 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
    38401 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
    38461 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
    38521 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
    38581 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
    38641 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
    38701 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
    38761 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
    38821 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
    38881 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
    38941 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
    39001 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
    39061 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
    39121 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
    39181 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
    39241 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
    39301 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
    39361 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
    39421 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
    39481 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
    39541 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
    39601 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
    39661 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
    39721 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
    39781 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
    39841 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
    39901 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
    39961 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
    40021 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
    40081 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
    40141 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
    40201 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
    40261 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
    40321 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
    40381 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
    40441 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
    40501 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
    40561 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
    40621 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
    40681 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
    40741 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
    40801 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacatttacc aattgagcaa
    40861 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
    40921 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
    40981 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
    41041 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
    41101 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
    41161 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
    41221 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
    41281 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
    41341 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
    41401 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
    41461 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
    41521 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
    41581 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
    41641 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
    41701 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
    41761 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
    41821 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
    41881 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgttga
    41941 aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt taattaacca
    42001 aaatattaat aatgattaaa acaaataatt taatataact tttattaaca aatatgttaa
    42061 tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa cgaagaatcc
    42121 ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca gctactttcc
    42181 aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag tgctatcacg
    42241 acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa agtaggacaa
    42301 caaaaaattt cgagccttcg tgtcgataaa atcgatatca gttttccaga aaaaatattc
    42361 aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa ttatgcaatt
    42421 gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga tgaaccacgc
    42481 tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag cggatttgtc
    42541 gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt ggaagtttct
    42601 gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga tatcactttc
    42661 agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc tgatatccgc
    42721 ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt gctgaagacg
    42781 atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa catcacgatc
    42841 aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa agacgatgcc
    42901 aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga tcaggatatc
    42961 aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac aaaaacagcc
    43021 aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat catccaaaac
    43081 ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga ttttttcttg
    43141 tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat atgtgacacc
    43201 tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag tcggaatttt
    43261 tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt gtcgtttgtc
    43321 caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga agaatccagt
    43381 aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc cagctacggc
    43441 atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat cgctgattcc
    43501 cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga tatttactcg
    43561 cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt catctgcttg
    43621 ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg tttcttcacg
    43681 catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt ggccgccgct
    43741 gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt tcataaagtc
    43801 aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta attcattgat
    43861 aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag ttctgctacg
    43921 ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt agacagatcg
    43981 actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga atctagattt
    44041 tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct ttttccttcg
    44101 ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt attgataaac
    44161 gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc atcccataat
    44221 gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca cacttgtttt
    44281 ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc catcattttt
    44341 ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg ctgatcatag
    44401 gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg catctttaaa
    44461 ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc cgtaaatccg
    44521 gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc actgtcgatg
    44581 cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat cgcacttggc
    44641 acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt atgggtttta
    44701 gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga atgagcatgg
    44761 gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt tgcatctgct
    44821 ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg tgcccgtcta
    44881 gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc tgcttttgtc
    44941 gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa ttggcagtgc
    45001 gctaaaggcc cttccacttg gattaatggt tcatttgcaa aaactagttc tccttcacga
    45061 gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa accttcagga
    45121 tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag attctctaaa
    45181 taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa aggcatttct
    45241 cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca gtatgtctgc
    45301 atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg gtaagtatta
    45361 ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata acaaaaaaaa
    45421 ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac ggatatttct
    45481 tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt aacctaaacg
    45541 agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc gttttctatt
    45601 tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt aagataccga
    45661 tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa gtttttgaag
    45721 caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat aacttattat
    45781 gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt gaaaaaactc
    45841 gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc tgtcaataga
    45901 atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt gtatacatta
    45961 gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga atccttcgta
    46021 tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat tccggcacgt
    46081 tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat ttttaaaacg
    46141 tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt gatcatcacg
    46201 acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa cgaaaataat
    46261 ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg ataaaatgtc
    46321 ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt cctcttttct
    46381 atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc attaaggaat
    46441 cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta atagtataac
    46501 ataatttcgt gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt aaaggattat
    46561 gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg tcagagatat
    46621 caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta aaaccagcta
    46681 ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt ctgcaacaca
    46741 tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc ttagacaatc
    46801 catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg gaaaaacggg
    46861 cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac gaaggaggaa
    46921 gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg aaagaaaatg
    46981 aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt acagcatcca
    47041 atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact gttgaaaagc
    47101 agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc ttagcagacc
    47161 gtatcttcca agctcataag aagtggttct cttacagctg gcctgaatat cagccaaaag
    47221 cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca tattatgacg
    47281 agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa aaggcgtaat
    47341 cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa acagcagaca
    47401 cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac aagttttttc
    47461 atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca ctttatcctt
    47521 tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat ggtggtcgat
    47581 ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca ccactggtgt
    47641 cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat ttgtaaagac
    47701 accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct ctgcatctat
    47761 acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac atagctgcaa
    47821 agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc ccaattgctt
    47881 tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc gatcaataaa
    47941 gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc catatttttg
    48001 atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc catcgaaagc
    48061 tttagctgca aattcttcaa gataccttcg ctctttttct ttatgatagc ccgtcggtaa
    48121 aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga tcaactgatc
    48181 aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt ttccataact
    48241 taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg caagtatttc
    48301 tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa tagtagaaac
    48361 tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt cgccacctat
    48421 acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa aaactgatac
    48481 atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc ttttgtcaaa
    48541 ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc ttgaaaactg
    48601 tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt gttcgttgtg
    48661 ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac tttgagtata
    48721 gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt tttattcctc
    48781 tttcattatt accaaagtct cttattttaa attgaataca ttataatgat actgtaaata
    48841 tatctttatt attagtgaat g
//
LOCUS       DACIWG010000020        48678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.020, whole genome shotgun sequence.
ACCESSION   DACIWG010000020 DACIWG010000000
VERSION     DACIWG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.020"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(148..2301)
                     /locus_tag="IYF54_001124"
     CDS             complement(148..2301)
                     /locus_tag="IYF54_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ9831467.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IYF54_001125"
     CDS             complement(2595..3443)
                     /locus_tag="IYF54_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9831468.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IYF54_001126"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IYF54_001126"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ9831469.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IYF54_001127"
     CDS             complement(5944..6123)
                     /locus_tag="IYF54_001127"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ9831470.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IYF54_001128"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IYF54_001128"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ9831471.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IYF54_001129"
     CDS             8271..10550
                     /locus_tag="IYF54_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9831472.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IYF54_001130"
     CDS             complement(10643..11290)
                     /locus_tag="IYF54_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831473.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IYF54_001131"
     CDS             complement(11469..12188)
                     /locus_tag="IYF54_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9831474.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IYF54_001132"
     CDS             complement(12319..13470)
                     /locus_tag="IYF54_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ9831475.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IYF54_001133"
     CDS             complement(13687..14427)
                     /locus_tag="IYF54_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9831476.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IYF54_001134"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IYF54_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ9831477.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IYF54_001135"
     CDS             complement(14811..15404)
                     /locus_tag="IYF54_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9831478.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IYF54_001136"
     CDS             complement(15388..16035)
                     /locus_tag="IYF54_001136"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ9831479.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IYF54_001137"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IYF54_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ9831480.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IYF54_001138"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IYF54_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ9831481.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IYF54_001139"
     CDS             complement(17481..18008)
                     /locus_tag="IYF54_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ9831482.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IYF54_001140"
     CDS             complement(18188..18973)
                     /locus_tag="IYF54_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ9831483.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IYF54_001141"
     CDS             complement(18966..19835)
                     /locus_tag="IYF54_001141"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ9831484.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IYF54_001142"
     CDS             complement(19836..21215)
                     /locus_tag="IYF54_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ9831485.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IYF54_001143"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IYF54_001143"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ9831486.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IYF54_001144"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IYF54_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ9831487.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IYF54_001145"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IYF54_001145"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ9831488.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IYF54_001146"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IYF54_001146"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ9831489.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IYF54_001147"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IYF54_001147"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ9831490.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IYF54_001148"
     CDS             complement(25173..25397)
                     /locus_tag="IYF54_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ9831491.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IYF54_001149"
     CDS             complement(25450..26412)
                     /locus_tag="IYF54_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ9831492.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IYF54_001150"
     CDS             complement(26422..26862)
                     /locus_tag="IYF54_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9831493.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IYF54_001151"
     CDS             27168..28133
                     /locus_tag="IYF54_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ9831494.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IYF54_001152"
     CDS             complement(28190..28420)
                     /locus_tag="IYF54_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ9831495.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IYF54_001153"
     CDS             complement(28506..29393)
                     /locus_tag="IYF54_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ9831496.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IYF54_001154"
     CDS             complement(29482..30705)
                     /locus_tag="IYF54_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9831497.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IYF54_001155"
     CDS             complement(30722..31801)
                     /locus_tag="IYF54_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9831498.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IYF54_001156"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IYF54_001156"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ9831499.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IYF54_001157"
     CDS             complement(33659..33925)
                     /locus_tag="IYF54_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ9831500.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IYF54_001158"
     CDS             34404..36638
                     /locus_tag="IYF54_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ9831501.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IYF54_001159"
     CDS             36786..37106
                     /locus_tag="IYF54_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ9831502.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IYF54_001160"
     CDS             complement(37311..37535)
                     /locus_tag="IYF54_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ9831503.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="IYF54_001161"
     CDS             complement(37733..39313)
                     /locus_tag="IYF54_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ9831504.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39714..41090)
                     /locus_tag="IYF54_001162"
     CDS             complement(39714..41090)
                     /locus_tag="IYF54_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ9831505.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41218..42336)
                     /locus_tag="IYF54_001163"
     CDS             complement(41218..42336)
                     /locus_tag="IYF54_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9831506.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42478..42912
                     /locus_tag="IYF54_001164"
     CDS             42478..42912
                     /locus_tag="IYF54_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831507.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42990..43523)
                     /locus_tag="IYF54_001165"
     CDS             complement(42990..43523)
                     /locus_tag="IYF54_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ9831508.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43617..44795)
                     /gene="mutY"
                     /locus_tag="IYF54_001166"
     CDS             complement(43617..44795)
                     /gene="mutY"
                     /locus_tag="IYF54_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ9831509.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44788..45585)
                     /gene="recX"
                     /locus_tag="IYF54_001167"
     CDS             complement(44788..45585)
                     /gene="recX"
                     /locus_tag="IYF54_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ9831510.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45692..47074
                     /gene="rlmD"
                     /locus_tag="IYF54_001168"
     CDS             45692..47074
                     /gene="rlmD"
                     /locus_tag="IYF54_001168"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ9831511.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47248..47826)
                     /locus_tag="IYF54_001169"
     CDS             complement(47248..47826)
                     /locus_tag="IYF54_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ9831512.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48148..48618
                     /locus_tag="IYF54_001170"
     CDS             48148..48618
                     /locus_tag="IYF54_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ9831513.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT        13850 a        10352 c         8441 g        16035 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcacatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    39721 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    39781 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    39841 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    39901 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    39961 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    40021 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    40081 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    40141 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    40201 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    40261 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    40321 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    40381 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    40441 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    40501 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    40561 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    40621 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    40681 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    40741 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    40801 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    40861 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    40921 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    40981 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    41041 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    41101 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    41161 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    41221 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    41281 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    41341 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    41401 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    41461 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    41521 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    41581 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    41641 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    41701 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    41761 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    41821 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    41881 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    41941 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    42001 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    42061 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    42121 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    42181 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    42241 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    42301 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    42361 tagtatatat tatattcgtt aaaaattctt ttattctttt tttatttttg aaaacttgcc
    42421 atacttttta tttgttttat tttccagtat actatacgaa aggaaggtgg ctagaccatg
    42481 aaagttgtac aaacaaaaga tacaatgagc aatatttact tagatgctgt acggatcagg
    42541 catcaagttt ttgtagtcga acagggtgtg cctttgtctc gagaaataga caaagatgaa
    42601 gcccattgta tccacttcgt cctgtactca gataaaaaag aaccgcaagg aactgtacgt
    42661 cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga tggcgattct ttctgaatac
    42721 agacaccaag ggttaggaaa aatattgatt gaagaagcag aaaactttgc taagaatcaa
    42781 ggctataata cgattttatt aggggcacag tccacagctg aaacttttta cgaaaaacta
    42841 ggatatacag cttacggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa
    42901 aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc
    42961 tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta
    43021 taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc
    43081 agaatcttca cattttcttt taggatgaag tcaatatcgt tcgggtaatt catcattttg
    43141 ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg
    43201 atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct
    43261 agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat
    43321 tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca
    43381 gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg
    43441 gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac
    43501 tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt
    43561 ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag
    43621 aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta
    43681 ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta
    43741 aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga
    43801 tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc
    43861 ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct
    43921 ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt
    43981 ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct
    44041 ccagaatgat tttgaagagc tgcactgata taataaacgt cttttggctt tgcttttttt
    44101 gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc
    44161 ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt
    44221 gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag
    44281 attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact
    44341 cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa atgcgatact tgcaatcgcg
    44401 cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt
    44461 tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag
    44521 taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct
    44581 tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg
    44641 gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa
    44701 ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt
    44761 tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa
    44821 tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt
    44881 tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa
    44941 ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagctatcg cttgttggat
    45001 cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt
    45061 ttggcttttt ccgtgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac
    45121 ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt
    45181 ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg
    45241 cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac
    45301 gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt
    45361 ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc
    45421 gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg
    45481 atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg
    45541 atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga
    45601 acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata
    45661 aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca
    45721 aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa
    45781 acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac
    45841 gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga
    45901 tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt
    45961 agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt
    46021 caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg
    46081 gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga
    46141 agctggttta tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca
    46201 agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc
    46261 acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg
    46321 tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa
    46381 aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa
    46441 tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa
    46501 agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt
    46561 tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt
    46621 ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt
    46681 agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc
    46741 aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga
    46801 gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc
    46861 accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa
    46921 acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa
    46981 aaagttcaag attgaatatt tacagtccgt cgacatgttt cctcagactg cacgttgtga
    47041 agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc
    47101 cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaatactg
    47161 tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat
    47221 agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa
    47281 tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc
    47341 ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat
    47401 ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt
    47461 ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt
    47521 taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg
    47581 ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc
    47641 ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc
    47701 tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg
    47761 aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt
    47821 tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca
    47881 caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca
    47941 gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaatc aaatcatatg
    48001 atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatta
    48061 tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa
    48121 ctaatattat agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt
    48181 tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga
    48241 aaatattact attctttgca acttccacca tttgcaccac ctagctggat atttgggccg
    48301 atgtggacct tactgtatat attgatgggt atttctcttt atttgttact taatcacaac
    48361 aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat ttttaatttt
    48421 atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca
    48481 cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctgcatca tcgcctggca
    48541 atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt gaattattca
    48601 atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag
    48661 tgccacctaa gttagatt
//
LOCUS       DACIWG010000021        45107 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.021, whole genome shotgun sequence.
ACCESSION   DACIWG010000021 DACIWG010000000
VERSION     DACIWG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.021"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(474..836)
                     /locus_tag="IYF54_001171"
     CDS             complement(474..836)
                     /locus_tag="IYF54_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831514.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(910..1290)
                     /locus_tag="IYF54_001172"
     CDS             complement(910..1290)
                     /locus_tag="IYF54_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ9831515.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1372..2547
                     /locus_tag="IYF54_001173"
     CDS             1372..2547
                     /locus_tag="IYF54_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9831516.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2624..3208)
                     /locus_tag="IYF54_001174"
     CDS             complement(2624..3208)
                     /locus_tag="IYF54_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9831517.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3409..4407
                     /locus_tag="IYF54_001175"
     CDS             3409..4407
                     /locus_tag="IYF54_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9831518.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4519..5028
                     /locus_tag="IYF54_001176"
     CDS             4519..5028
                     /locus_tag="IYF54_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ9831519.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPSILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5058..5717)
                     /locus_tag="IYF54_001177"
     CDS             complement(5058..5717)
                     /locus_tag="IYF54_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ9831520.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5719..6483)
                     /locus_tag="IYF54_001178"
     CDS             complement(5719..6483)
                     /locus_tag="IYF54_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ9831521.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6491..7168)
                     /locus_tag="IYF54_001179"
     CDS             complement(6491..7168)
                     /locus_tag="IYF54_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ9831522.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7205..8590)
                     /locus_tag="IYF54_001180"
     CDS             complement(7205..8590)
                     /locus_tag="IYF54_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ9831523.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8800..8884)
                     /gene="ffs"
                     /locus_tag="IYF54_001181"
     ncRNA           complement(8800..8884)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IYF54_001181"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8907..9422)
                     /gene="tadA"
                     /locus_tag="IYF54_001182"
     CDS             complement(8907..9422)
                     /gene="tadA"
                     /locus_tag="IYF54_001182"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ9831524.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9529..10386
                     /locus_tag="IYF54_001183"
     CDS             9529..10386
                     /locus_tag="IYF54_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ9831525.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10467..10802)
                     /locus_tag="IYF54_001184"
     CDS             complement(10467..10802)
                     /locus_tag="IYF54_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831526.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10869..12239)
                     /locus_tag="IYF54_001185"
     CDS             complement(10869..12239)
                     /locus_tag="IYF54_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ9831527.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12317..12853)
                     /locus_tag="IYF54_001186"
     CDS             complement(12317..12853)
                     /locus_tag="IYF54_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831528.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13162..14643
                     /locus_tag="IYF54_001187"
     CDS             13162..14643
                     /locus_tag="IYF54_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9831529.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14746..15453)
                     /locus_tag="IYF54_001188"
     CDS             complement(14746..15453)
                     /locus_tag="IYF54_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ9831530.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15532..15870)
                     /locus_tag="IYF54_001189"
     CDS             complement(15532..15870)
                     /locus_tag="IYF54_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ9831531.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15981..16943)
                     /locus_tag="IYF54_001190"
     CDS             complement(15981..16943)
                     /locus_tag="IYF54_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ9831532.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16958..18289)
                     /locus_tag="IYF54_001191"
     CDS             complement(16958..18289)
                     /locus_tag="IYF54_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9831533.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18297..18977)
                     /locus_tag="IYF54_001192"
     CDS             complement(18297..18977)
                     /locus_tag="IYF54_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ9831534.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19196..19675)
                     /locus_tag="IYF54_001193"
     CDS             complement(19196..19675)
                     /locus_tag="IYF54_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ9831535.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19802..21481)
                     /locus_tag="IYF54_001194"
     CDS             complement(19802..21481)
                     /locus_tag="IYF54_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ9831536.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22029..23531
                     /locus_tag="IYF54_001195"
     CDS             22029..23531
                     /locus_tag="IYF54_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831537.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23544..24206
                     /locus_tag="IYF54_001196"
     CDS             23544..24206
                     /locus_tag="IYF54_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831538.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24307..24510)
                     /locus_tag="IYF54_001197"
     CDS             complement(24307..24510)
                     /locus_tag="IYF54_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ9831539.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24621..25805)
                     /gene="iadA"
                     /locus_tag="IYF54_001198"
     CDS             complement(24621..25805)
                     /gene="iadA"
                     /locus_tag="IYF54_001198"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ9831540.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25962..27701)
                     /gene="yfcC"
                     /locus_tag="IYF54_001199"
     CDS             complement(25962..27701)
                     /gene="yfcC"
                     /locus_tag="IYF54_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ9831541.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28473..29087)
                     /locus_tag="IYF54_001200"
     CDS             complement(28473..29087)
                     /locus_tag="IYF54_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9831542.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29693..30070)
                     /locus_tag="IYF54_001201"
     CDS             complement(29693..30070)
                     /locus_tag="IYF54_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831543.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30370..31251)
                     /gene="galU"
                     /locus_tag="IYF54_001202"
     CDS             complement(30370..31251)
                     /gene="galU"
                     /locus_tag="IYF54_001202"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ9831544.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(31294..32313)
                     /locus_tag="IYF54_001203"
     CDS             complement(31294..32313)
                     /locus_tag="IYF54_001203"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ9831545.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(32946..33950)
                     /locus_tag="IYF54_001204"
     CDS             complement(32946..33950)
                     /locus_tag="IYF54_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9831546.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(33963..35012)
                     /locus_tag="IYF54_001205"
     CDS             complement(33963..35012)
                     /locus_tag="IYF54_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9831547.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(35029..35850)
                     /locus_tag="IYF54_001206"
     CDS             complement(35029..35850)
                     /locus_tag="IYF54_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9831548.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(35837..36616)
                     /locus_tag="IYF54_001207"
     CDS             complement(35837..36616)
                     /locus_tag="IYF54_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9831549.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(36633..37103)
                     /locus_tag="IYF54_001208"
     CDS             complement(36633..37103)
                     /locus_tag="IYF54_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831550.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(37115..37534)
                     /locus_tag="IYF54_001209"
     CDS             complement(37115..37534)
                     /locus_tag="IYF54_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ9831551.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(37615..40383)
                     /locus_tag="IYF54_001210"
     CDS             complement(37615..40383)
                     /locus_tag="IYF54_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9831552.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            40583..40807
                     /locus_tag="IYF54_001211"
     CDS             40583..40807
                     /locus_tag="IYF54_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831553.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            41586..43301
                     /locus_tag="IYF54_001212"
     CDS             41586..43301
                     /locus_tag="IYF54_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831554.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            43304..45067
                     /locus_tag="IYF54_001213"
     CDS             43304..45067
                     /locus_tag="IYF54_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831555.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT        13386 a         9321 c         7612 g        14788 t
ORIGIN      
        1 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
       61 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      121 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
      181 ataactattt tttaaatttt ttagaaatgt gatataacat ccacttcaga atcaacgctg
      241 ttaaatttat catgtatttc ttccttggtc aactataaat taagcattac tttgtgaaat
      301 gatttttcga tcaattatta ttattcgtta tacaaccgtt ttcaacatat tttgaaaata
      361 aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg accaaacaat
      421 aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag
      481 aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc
      541 caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac
      601 aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta
      661 acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct
      721 tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt
      781 ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt
      841 tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa
      901 atacactttt tatttcttat tttctttccg ttctagttca gataatgctt cttctatcca
      961 aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat tcgtgaaata
     1021 cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta aagataaact
     1081 gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct gtccgacttt
     1141 taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat gaataagtcc
     1201 ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg tcacttttcc
     1261 ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat ttatcattat
     1321 actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa
     1381 tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc aattgctgag
     1441 acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt cttgtctccc
     1501 ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg ttatgccact
     1561 ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca tgctgtggag
     1621 ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta
     1681 caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta tcctccattt
     1741 tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat
     1801 aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa
     1861 gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa agaagaattg
     1921 aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga cgagatccat
     1981 caagatttaa ctttattcga tcatacattt acttccatgc taacgattga tcctaattta
     2041 gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc tgcaatcaaa
     2101 aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa acatttagtg
     2161 atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc ggcttatgag
     2221 acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt
     2281 aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga aggaacttac
     2341 ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga gaacactctt
     2401 gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc cggtcatggg
     2461 catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact taatcgaatc
     2521 aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga cgagacctta
     2581 ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct gaaatttcaa
     2641 ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt acatttgcga
     2701 tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt cggaaatcta
     2761 gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat ccagcacctt
     2821 ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag aattggcttc
     2881 cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg aacagttcac
     2941 gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt cctgttccag
     3001 ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc acgccatcat
     3061 aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct agttcaggga
     3121 ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt cctttttcac
     3181 tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc atacttcata
     3241 ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt cttttatttt
     3301 tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga
     3361 agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat ggaaatctat
     3421 gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg
     3481 cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact
     3541 ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa
     3601 ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa
     3661 gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc
     3721 cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg
     3781 aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg
     3841 atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg
     3901 acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt
     3961 cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc
     4021 tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa
     4081 gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc
     4141 tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact
     4201 gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc
     4261 tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac
     4321 aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta
     4381 tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc
     4441 cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa
     4501 taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa
     4561 ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa
     4621 aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta
     4681 acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat attagcagaa
     4741 aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt
     4801 atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat
     4861 tatggttata tcgacaaggt gaaacctagt attttaaaag aactaaacag ccacgatggc
     4921 gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt
     4981 cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa
     5041 agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtttcaa gctatttttt
     5101 cctaagaaaa gaaaaacagc aaaaaataat tctaataata caaaaaataa gataaagcta
     5161 tgcatgaaaa attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac
     5221 cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc
     5281 atcatgaaaa aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac
     5341 cgccaaagcc gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg
     5401 atgaaaaaca cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct
     5461 ccgctagcag aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga
     5521 taattcatta ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc
     5581 aaaatttgca aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca
     5641 actgccagac tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc
     5701 catctcgaat ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc
     5761 tgggataggc aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt
     5821 atctatatta ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat
     5881 tacttcttct ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg
     5941 ttttccgatg tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc
     6001 tgctccagga agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa
     6061 agtagcacct ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt
     6121 atctaagcct accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa
     6181 aatcaagtca atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc
     6241 tttcataaaa tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat
     6301 gtcaaactca tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt
     6361 aacaaacaag aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg
     6421 tatgggttgt gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc
     6481 cattcatcca ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt
     6541 gtttaggttg ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat
     6601 ttttaataac cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg
     6661 cacgattatg gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga
     6721 ttctctcgat gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca
     6781 aggaatcgat tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta
     6841 ggcgtagttg ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac
     6901 tgtaacgttc tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat
     6961 aatgtctttc acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga
     7021 ctttttctgc tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga
     7081 ctgttgtttc ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt
     7141 tctgctgttc attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt
     7201 gtttttattt gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt
     7261 cagggaacaa aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca
     7321 tcaaatgatc tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc
     7381 aaaagacttg atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa
     7441 tcttaccgcg aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt
     7501 ctatttctaa aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca
     7561 tcggatgcgg caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag
     7621 ttaaaaacag accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct
     7681 caaaggctac ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca
     7741 aatctgctac ttctttttct tttaataatt catgcccacg ttctagataa ctatttattt
     7801 cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct
     7861 taaggatttt tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact
     7921 ttccagcggc tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg
     7981 tgtgcgatcc taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct
     8041 gaatcccgag atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta
     8101 attctggcaa aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg
     8161 gaaatggagc agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt
     8221 tatatggttt cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga
     8281 tgatatcaaa ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc
     8341 cttcattgtt tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt
     8401 cctgtccatt cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta
     8461 ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc
     8521 ttctgatcct tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta
     8581 ttttttccat gtaccctctc gcctctcttt atctttatca taccataggc agctttttct
     8641 tccattgaaa caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac
     8701 aatattcgat aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa
     8761 aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg
     8821 aacctgctta gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct
     8881 agcctttcgg caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat
     8941 ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct
     9001 tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc
     9061 atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca
     9121 cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca
     9181 gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct
     9241 gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt
     9301 ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct
     9361 tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc
     9421 aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg
     9481 taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat
     9541 aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat
     9601 atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa
     9661 caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca
     9721 tggtatcact atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc
     9781 cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg
     9841 gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc
     9901 gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta
     9961 gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc
    10021 gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt
    10081 tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat
    10141 actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg
    10201 gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca
    10261 ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt
    10321 ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat
    10381 aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat
    10441 gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg
    10501 tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt
    10561 cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca
    10621 tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact
    10681 ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct
    10741 gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc
    10801 ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac
    10861 taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact
    10921 tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca
    10981 tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag
    11041 caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga
    11101 ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa
    11161 ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct
    11221 tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc
    11281 actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc
    11341 agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt
    11401 tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga
    11461 atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct
    11521 tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt
    11581 gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac
    11641 tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc
    11701 acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc
    11761 actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc
    11821 ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt
    11881 gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca
    11941 gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg
    12001 aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat
    12061 tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga
    12121 aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct
    12181 aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt
    12241 ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac
    12301 tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg
    12361 tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat
    12421 ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga
    12481 tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta
    12541 aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata
    12601 agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa
    12661 cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa
    12721 tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc
    12781 catattcttc cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta
    12841 ctctccccat cattcttacc tccgtctctt ttcatctacc cataaataaa taacataaat
    12901 aattctaaaa atattattga taattatcaa aacatattta gttatatcca ttaaaaatca
    12961 atttttccgc cttaaaaaca agaatatctt gacaaaatac cctaggaaat aaacaataga
    13021 ataaacaagg taacaaaaaa attaaataac gttatataaa aaagaaggcg acactctgtg
    13081 agaataacac agactgaaat gactttccac tcacagtttt tgtctaacta tatacaaaaa
    13141 cagaataaaa aggaggtatt tatgctaatt gaagcaagaa aaaaagagtt acttgttcta
    13201 caaatggtca ttcaaaaaaa taattgttct ttacaagaat tatccaatga cttacatatt
    13261 ccaaaacgga caatcaaaga actgatccga aaaataaatg tatcactcaa gcaattttta
    13321 ttgattgatg aatttattta ttctaatcat aaaggagaga tccaaaccaa agaaaccgat
    13381 caaactaaaa agttagctgt tttttctaag ttaaaattat gctacctaaa agaatccaat
    13441 cgttttaact atttgatgtt actaatcaat tatccacaaa ctgctgtacc aaaaaaatat
    13501 ttacttgaac aactttatat atcgccctcc tatctcgaaa aactgacaaa acagctcaat
    13561 cattccttaa aaaaattcca tttacggatc ctatctgtaa aaaattgctg tactcttgaa
    13621 ggaaatgagt tatctatccg tctctatctt tacttttttc tttcagatac atttggaggt
    13681 aacgattggc cattttcctc acttgaatta aatgcactaa aaatgaacag tacagatgag
    13741 caaatagaaa aaatttcaaa acaagaaaaa aatatctact tgctgttaac tttattcata
    13801 ttacgaaaca aacaacaggc atattttcca aaaacttctt ctgaaatcac tgaattgatg
    13861 gacttattgc agcatacaca tgattattca gaatacttta cttctttttc agtgagtcgc
    13921 ccttgtgaag agacagaaac agagagagcc tactttaatt attttttcta ttgttttttt
    13981 ccaaatcaaa tctcttttcg acaaaaacag gagattggtt ttttattttc cacaagcaaa
    14041 aaccttattt gtcgacaagt cggtcaattg atgacttact taactcaaca gtttcctctc
    14101 aaccatttag aaaataaaat accaatttat ttttattttc ttgtgttggc agttgttttt
    14161 ttcgaactgt tgggtgaatc cagcagtagt ttttctacac tttgctttcc agaagccacg
    14221 tccaatcatt ctagttcttt tatacaggaa aagaaattaa gagaaaaaat cgatacaatt
    14281 ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc ttagtcaatt gatccaacac
    14341 ttactacaaa cagaaacgaa agagcagtta ctcatccatt tgcagataac caaacagttt
    14401 acaggaaatt atcatcttca aagccatttg cagcagttat ttcgcaaaga tgctgttaaa
    14461 attaccgcat attcagaaca tgctgatttg atcattacag atcagcatga gaaaatttca
    14521 gatgaaacgg agactttcta tctggataca acgcacaata cagtgttatt tggcaagtta
    14581 ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg agaacaaaac aaagaggatg
    14641 tgacaaaatt cttgtcacat cctcttattc caaataaatg gtattcaatc agctaactct
    14701 cggtattagc tcagatcaaa gcgctgattt cacaacttct ttattttaat ctcttcggtt
    14761 gccgccgaac aataagatca agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac
    14821 ataagtcagt gctgctgcaa ataatacgtg tcttgccatc ggtacttctt cttccgtcag
    14881 tagtccgcct tcactcaaaa tttggattgc tcgccgagac gcgttaaatt cgactggtaa
    14941 tgtcaccaat tggaaaagta atgctaaaga aaaagcgaaa atcccgatat tgattaacgt
    15001 agagttccag ctaagcaata cacctaccat aatcaagggg aacgaaatca tcgaaccaaa
    15061 gttagctgct ggaacaagtg ctgcacgaat cttcaatggg acataacccg aagcatcttg
    15121 taccgcatgt ccgcattcat gagctgctac acctatcgca gcaactgaag tagactgtgc
    15181 agttgcttga gacaaactca acatcttatt acctgaattg tagttatctg tgagatctcc
    15241 ggcaatttgt tgaacaccga cattatttat ctgttgactt tgtaatatat attgtgctgc
    15301 ttgtgtacca gtcacattgt ttttgcttct cacttgatcg tattttcgga aagtactatt
    15361 gacgtacgct gacgcaattc cggaaataac taatccaaga atgaccaaaa gaaatgtcgg
    15421 atctaaaaaa ccatacattc cataaccata cattttcgtt tcactccttt tcgtttactt
    15481 tattttatca ttagtagtag gaaaagaatg tgaaaagacc ttgaatattc tttaattctt
    15541 tttcacaaat tccgatttca gtttcatcgg tccaaatcct tctaccttgc aatcgatatt
    15601 atgatcgcct tctactaaac ggatgttctt gacttttgtt ccttgtttaa tggcacctga
    15661 cgcacctttt actttcaagt ctttaatcac tgtcacgcta tcgccctcta acagacggtt
    15721 tccatttgca tctaatacca ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt
    15781 ccattcgttc ccgcattctg ggcaaatcaa caagcctcga tcttcatatg tgtactctga
    15841 gccacattct gggcaatttg gtaatgtcat cttctttctc ctttaaaatc tttagtgagc
    15901 actattgtaa cggaaaaaga agatcttgaa aagatacatt tgtgattttt actacaaaag
    15961 gcacgttttc gaacgaagta tcattctgtt tttctgctct tatacgattg gcatatatct
    16021 tggatctctt tatagatttc ctctacttct gctaaatctt ttgcattcgc accacttgct
    16081 aatggatggc ctcctccatg atggcgttta gccaattcat tgatgatcgg ccccttagaa
    16141 cgtaaacgta cgcggtaata ccctgaaggc tgctgaacaa aaatccccca agccagcact
    16201 tcgtcaatca ctccaggaag tgaaacgatc gaagctgtct ctgaatcatc gattccatat
    16261 ttatcaagaa tttcttgcgg aaggattatt cttcctgcac cattttcatc cacctggata
    16321 ttttgataaa gataaccgga aagtttagcg actttcatcg gcatttgttc aatctctctg
    16381 ttcaaagcac ttgcatcaaa tggataattt ctcaatatcg aagcaatctc taatgtacgg
    16441 gacgtcgtgg agggatacaa aaagcgccct gtatctccga cgattcctgc ataaagtaac
    16501 cgtgcagcct taacgtttat ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc
    16561 atctcactac agctacttgc ttttgtattt acccacacaa gatctccata agggtcatca
    16621 ttgggatgat ggtcgatttt gatcagtttt tctgccaact gatatctttg atcactgatt
    16681 ctgggtgaat tagctgtatc agtcacgatg actagcgctc cttggtagaa gacatcttca
    16741 acttcttgca tttctgctaa aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt
    16801 tctttttctg ggaaactttc ccgtaatagt tcggctaatc ctacttgtga tcctaatgca
    16861 tcaggatcag ggcgcaaatg cctgtggata atgattttat cgtatttttt tattgtatct
    16921 actatttctt gctgtactgt catttcactc ctgctttcta agaacgttcc attacttgac
    16981 aaacaacgat tgctttggaa acgatggtgt tttcaataaa cacttcgata tccagtttag
    17041 cggaacgtct gccgatttcc aatacacgtg gacgaatgtc taattcgctt tccaattgga
    17101 tcagccgcaa ataatgtagg ttcacttgtt caatcaagat atttcgtttt tggttcatca
    17161 ccattgtttt ttgtgcgaca tcagaaatga tttcgctaag tacgccaaaa gaaatggttc
    17221 caacgccatt gaccatttgt ggtgctacta cgaatttgaa cttagggttt tccaaacgat
    17281 ttccttcacg gtcagtatca atcgtatgga tatttccaga tatctgatcc gaaatcgtat
    17341 ctgcaatctg cggttgtcgc tggacaagct gcatcgcttt cattacatct tgccgggtaa
    17401 tcaagccttc caatgtcaag tcatcggaaa cgaccggcat gacttctagc ccatcccaga
    17461 tcatttgatg gctaacagag gcaatactca ttgttttctt tacactgatt ggttctttcg
    17521 tcatcacccg atcgatgatc tggtttggcg atttccctaa aacatctttt gccgtaacaa
    17581 tccccatcaa acgatgatgg cggttgacga ctggaaaacg cgaatgctgc gtttcttctg
    17641 acaaacgctg ataatcagaa atcgtatctg tctgctgcaa ataatgagtc ttttcagctg
    17701 gcatatagat atcactgaca agcatgatat cttttttgat caattggtcg cttaacgctc
    17761 ggttgatcat ggttgcgact gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa
    17821 gatcatcagc tagctttgca atctcagcag tcgtattgaa tcctcctgtg atcaatactg
    17881 cggcgccatc ttccaatgct aatttttgga cttccgcccg gttgccgacg atcattagtg
    17941 atcctggcgt aatatagcgt tccatggcat gtttagtcat cgcgccgatg acgaatttat
    18001 tcaagacctt gtctaatcct gccgccccgc ctaaaacgtc cccttcaatg atcctgacga
    18061 cctcgccaaa tgtcaaacgt tcaatatgtt tttttaattt gcgttcgata cggatggtcc
    18121 ctactcgttg aattgttgaa actagtccga cattttctgc atctttgatt gcacggtacg
    18181 ctgttccttc actgacattc aattcttttg cgatgcttct tacagaaatt cgattgccaa
    18241 caggtaaact ttcaatatat tctaaaatca aatcatgttt tgtcgccatt ttctgcctac
    18301 acttccagcg tttccgagac gctcattatt tttccttcac tatcttccag taactctaca
    18361 aaagattcgg gatcttgttc aatgacaggg aaagtattgt aatggattgg aactactttc
    18421 ttggcacgaa ttgtttgcgc agcctttgct gcatcttctg gccccatcgt atagttatcg
    18481 ccaatcggca agaaagcgac atcaatatcg aattcttctg caagcaatcc tagatcactg
    18541 aaataagcag tatcccctgc atgataaatc gtttttcctt ctgcttgaat aataattccg
    18601 ctaggctcgc ccatatactg aacgataccg tctacttcat agcttgaact atgctgtgca
    18661 tgtacgaatt ttaccacgcc gaatggaaac gcatgttttc caccgatatt cataccgaat
    18721 gaatctactc ctctgctgtt cgcataatca gcaatctcaa caatactgat aattgtggct
    18781 tgattttctt ttgcaatagg aatcatatct cctatgtggt cactatgacc atgagtaatc
    18841 agcaaccaat ctgtctttac ttcttctgca gttaagtctg tcaatggatt tcctgtaata
    18901 aacgggtcaa tcatcacgcg ttggccattt tccatttcaa ttgttaaaca ggaatgtcca
    18961 tgatacgtaa ttttcatatt ttacacctcg cgttttctct gtatcttttt tattaaaaac
    19021 gatttcatca cttgcacatc aatgaaaaaa tatagctgct aaatatattt tcattggatt
    19081 gatagtacag tatgtacaaa gcaattgtaa cagataatac aggatgcacc aagtaccaag
    19141 cagatataat ctcaagtttg aggtctctgc tctgcttagc ttctcttctc gtcccttaac
    19201 taattaaatc agcgtaaata ggatggacga tcttttctaa atcggctact tttatccgaa
    19261 ggcttctccc gatttcaccg cttgacacaa ttacttcttc tttttctttt aaagaactgt
    19321 ctgctaaaaa acaataatca ggatgtgcga gttttattcc aataggggtg tttgctccgt
    19381 ggggatatcc tgtatgcatt aatacaaaat ccattccagg aaaaccaact ttacgatctt
    19441 tcgtcacttg tctcgttttt ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga
    19501 tcaccccagt tttgtttcct ttcactacga gagttttaca tatcaaagct ggatcaatcc
    19561 ctcttttttg tagttcttct gtgaaagatt cttctaccac atgactaaaa tcaagtggtt
    19621 gatagctgat cttttgttct tctaaataac gttcgacttt tgtttgactc atcattgatt
    19681 cactcccttt attctatcgt ttattataaa gcgagggatg ctgtattcaa aaattatttg
    19741 tttttcgcaa aaaaagagca agacgagcac tctatagaga ggttcatctt actctttttg
    19801 tttatttttt atttcgacgt tgagtagctt ccataatgac tggcaagatc actgggcggc
    19861 gttttgtttg ttcaaataag taacggccta attgatctcg gatgtcttgc tttaatttgc
    19921 tccactcaaa atcattgctt tctaaatgtt tctcaacgat tgtttcaatc atattgctgc
    19981 tttctttcat cagatcacgg ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag
    20041 gttttgaaat gattttcttt tctcgccgac tgatagtgac aaccgctacg aaaatcccgt
    20101 cttctgacaa gatttttcga tcacgtaaaa caatgtttcc gatatcacct atgcctaatc
    20161 catcaatcat tacattttct gcacttgtag ttcctgcaac actcatacgc ttgttcttat
    20221 attccagcac gtctccgcgt ccagtaatat agatattttt gtatggcatc cctactgcat
    20281 gagccaaatc tgcatgagca gctaattcac gatactctcc ttgaactggc acaaaatatg
    20341 ttggtttcat caaattgatc attaattgca agtcattagg attggcatgg ccagaaacgc
    20401 gcatattctc agagatcaac ttcactgttc caccagctcg ataaatgata tcttccgttt
    20461 tcgccacgat ggtttccatc gcgatgcttg gcgtagtagt gatataaacc aaatctcctt
    20521 cttcaatctt gacattacgg tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt
    20581 cacccattcg gccagtttct aaaatgatca gttcttctgg atcatagttt ttcatgtctt
    20641 tcaacgtaac aagcagatct tcacttggta attgaagttt ttctaaacgc atcgctgtgc
    20701 gaataattcg ttcgaaatct tgaccagtca agacaacttt tcgttgtgct ttttctgctg
    20761 catccaatac ttgttgcact cgttgcaaat tacttgctac acaagcgacg atgatacgcc
    20821 cttcccaata tttgatcgta tcaaatactt catctgctat ttgggcttca gaagctacag
    20881 gctgaggatt ttctgcattt gccgattcac ttagtaaggc taagactcct tctttgccga
    20941 tttccgccaa tcgtgcataa tctgtttgat acattgggat agccgtttgg tcaaatttaa
    21001 aatctcccgt ataaacgata ctaccttctt ttgttttcag attgatcccc actgaatctg
    21061 gtatcgtatg agtggtacgg aaaaagctca ctactgtttc accaaagtct atttctgtat
    21121 gctcatctac cacatggaaa tccttaaatc ctttggctgc ttcattacga ctgacattca
    21181 attttgctaa ctcgatcgtc aactctgtcc cgaatactgg tacagaaatt tttgacaaca
    21241 agtaaggaag tgccccgatc gcatctgcat gtccatgtgt taaaaagaca ccagctacac
    21301 gatcaatgtt ttcttctaaa tacgtaaaat cagggattac gacgtcgatc cccaacaact
    21361 cattttcagg atattttagc ccacaatcta aaataaaaat atcctcgccc acttctgcga
    21421 catacatgtt ttttccattt tcacgaacgc cgcctaatgg gataatttta atggtactca
    21481 caaacttcac ctcttcttat gacatccaag tttaaagatg tcgattttct tctttatcta
    21541 aatatatgag ccattccaat gaagcatatg caaaatcact atttctcaac ttatggagaa
    21601 tcagcaggaa aattaccagc acatccgcgt tctgacagtt ggatgggcat tttattttgg
    21661 atatacttca ttaacaaaat ggatcttttg cgaaacgcta tagccagaat acttatataa
    21721 gtattcagct gttccccatt attatacaac aaataaacta aatcgctagt atcagcaatt
    21781 gttagaacaa ctatgcacgt aaaaatccct ctgtagcatc taggatttct tttgctattc
    21841 ctctttcatt tcttcttcgt cggtttgaaa catacttttt tcattttacc acattttttg
    21901 caataactat agtgactttt tatatataca gaacctcatg ttgatggtgt gcaaacagca
    21961 taagatttaa gctagcttta agataggttg tctattatca aatgcatcta ctaggaggga
    22021 tgcaaaatat gaattttatg aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt
    22081 cattgttatt gtttgccgtg ttcacagcca ttcttatttt tgtgctagct ggcctcacga
    22141 ttcgaagcgc tgcagaagaa gcagcgaatc aagcacaaaa agaagttgga gcaacagtga
    22201 ctttaagtgc caatcgagaa gcttcattta aaaaacagga acaaacttct tcaagcggtt
    22261 ctaccactga agaacgtcca gatccaggca gtttttctct tacaccagtt tccttaacgg
    22321 acgcggaaaa aatagctgaa ttagataatg taaagagtta ttcctttgaa agttccgcct
    22381 cagctatggc aaaagatgga ataactgcca tcactagttc tgattcaagt gaagacagtt
    22441 cagaaactac tgatgcaggc ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga
    22501 ctcaagcaga ctttcaaatc agtggtgtgt cagcaactgc acaaaatagt aatttttctg
    22561 atggaacagc aaaaatcacg gatggtgaag gaatagatga cagcgacaaa ggcacaaaca
    22621 atgtagtcat tgattctact ctagcagaaa caaatgacct ggcagtcgga gatactttca
    22681 caatcacgag tactgaagat gaagatacca cttatgaaat gacgatcaaa ggaatttatg
    22741 aatccagcga aacaagcggc agtatgggta tgaaattcaa cttcatgaat ccgtcgaata
    22801 cactttacac ttcttacact tttgcaaatg aactaaatgg gacatctgac gataacacga
    22861 tagattctgc tatttacact ctatctgatc cagataaaat ggatgagttt ctcaaagaag
    22921 ctgagaagct gatcgacaca gatacattta gcttacaatc aaatgattcc atgtatcagt
    22981 caatgttgga acctttaaac aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg
    23041 ctgtagccgg tattatcata cttactctga tcattatgat taccattcgg gagcgtcggc
    23101 atgagttagg cgtcttgcta tctttaggag aatccagaag taaaatcatt ctccaattgt
    23161 ttaccgaagt ggctatttgt atgattcttg cattaggagt tgcttcgctt agcggaaacg
    23221 tcgtcgcgaa tgccgtggga cagcagctac ttgaccaaca gacagaaacc acgacacaaa
    23281 accagcaagc tcctggcggt ggacaaatgc caggtaacag acaagaacaa ggcggtcaac
    23341 gcgatggtca acaaggaacc acgaataatc catttgctgt ttctgatcaa gtcagcgaat
    23401 tggaaatcaa tgtcaaacct gcacaattgg gactattagc cggagtagca tttggaatca
    23461 gtctgttttc tgtcttttta gcttctatag ggatattaag attaaaccca agaaaaatcc
    23521 tattaaacta aaggaggaat ttgatgttac ggacagaaca attgggattt tggtatcaag
    23581 atccgcaaga tgcgctttat aaagatgtag aactgaattt cgaagctgga aaaatgtacg
    23641 ctattttagg agcaagtggt tctggaaaaa cgacttttct ttcgcttatt tctggactag
    23701 atgcaccaaa agaaggaaag gtatactatc aagatcgttc actgaacaaa atcgggttaa
    23761 gaagttatcg taaaaatgat gtctcgatta ttttccaagc ttacaacttg cttccttatt
    23821 tatcggctct agacaacgtc atagcagcaa tggaaatcac taagaccaag caagagaatt
    23881 atcgacaagt tgctttagat cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa
    23941 aacaggtgac caaactttcg ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct
    24001 gctgtgaaca tcaactaatc gtagcggatg agcctactgg aaatctagat gaaacaactt
    24061 cacaagatat cgtcaaactt ttccaagaaa tcgcccatga acaaaatcgc tgtattatca
    24121 tcgtgactca tgagattgaa gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata
    24181 aaaaatttca aaaaatcgaa gtttaaaaaa caggataatg atcaaataaa aaatccgaac
    24241 catatctgtc tcgcagatat ggttcggatt ttttatttgc ttgattcaat tagtaataag
    24301 tggtttttat tttttttcgt cttttcgaga aaacttgtct ttgacttcgt cgcctaaatt
    24361 tcgagctttt tctttgatgt cagcacctag tgattgtgct ttcccttctg tttctttgct
    24421 cttgtcgtct gttgctttgc cgtatgcttc ttttccttca ccgactactt tatctttcat
    24481 atctttagaa cgtcctttta aatctgccat tgtgcttcct cctcttagtt tgtttattga
    24541 tctaccttca gggtaacatc aacaacagat acagacaaac agaacgctcg ttttctaaaa
    24601 aagatagtta gagctgttga ttaaaaactt ttttcgaaag tcccccatac tttgacttct
    24661 ttttcttgga ccattatttc tcctttagcg ataactgtat caatatcaag cgtttcttta
    24721 tccaagatac acagatctgc atcagcacca atttcgattt ttcctttttt atccaatttc
    24781 aatatacgtg ctggattact ggttatagct cgtaaggcga tttccaaagg gatactttct
    24841 ttttgcacag cttctttgat ccctactagc aatgccttgg cacttcctac tcctaaccct
    24901 aggaaatgat tgttttcatc aaaataaggc aagcttcctt gtccatctga ggtcatcgtg
    24961 aaattatcta aagaaacatc ttcctcgatc aatcgtttca gtgctttgct gaagcgaact
    25021 tcaccatctg tcttctccca aaaatcagga tcttcgctag cagtgaagtc aatcgtgcca
    25081 ccacgtttgg caaatgccac gcaagcttca aataattctg gtgtgcgatt tgcatgagta
    25141 ggcaaaaatt gtgtgatcgg aatttctgtt tcttctacga ttcgtgtcaa tagctctagc
    25201 tttctttttc ctccgcctaa gtggacattg atgatcccag cttttccgga taacatgcct
    25261 cctacacgag catctgctac tgcacgagcg aactcttcaa aacttggctg agaagaacgg
    25321 tgatcggata ctgcgatttc tccgattccg ataattttat caattgtcaa aaagtctttg
    25381 atgatagaat ctgttactgt ttttaccggc aatcggtacg aaccttcgta tacataagtg
    25441 gagattcctt ctgcttccag acctttcgct ttgctgatca acgcagtcat atctctacct
    25501 tctccgtctg ttcccaaaca accgacaact gtcgttacac cagctgtagt caattggctc
    25561 aatgtgactt ctggcgttcg attttgataa ccgccttctc ctcctccacc taaaatatgg
    25621 aaatgacagt caatgaaacc cggggttagt acttttccct cacctgaaag ttcttctact
    25681 tccactcctt cgtagccgcc ttctagctgt tcttctacag ccaaaatctt cccaccggca
    25741 atcagtacat ccatttttcc taagtaatct ggtgcaaaca cttcaatttg tcgaatgatt
    25801 ttcatacatt tttcctcttt ctttaagtat aactgatgat aggatcttaa aaaataatcc
    25861 aaacgatagg atctttactc ctttgatttt accgtaaaat gacggttctt ggagtcctta
    25921 tctactgttt ggattacttt tttatttaca ggtctccagt actactgcag acctattgca
    25981 atggcaatca tgatgaaaat agttcctaaa acaaacagga atccttgcat cttgatttgg
    26041 aatcttgccc attttgccca ttcgatccgt gcaacaccta gcacaccaat caagctggct
    26101 gatacgggtg taaaagcgtc cataaaacct gaaccaagct gataagcaag aactgctact
    26161 tgtctggata cacctactaa atctgccagt ggagccatga ttggcatagt caaagctgct
    26221 tgtcctgaat ttgatgtaac aaccaagttg aacaaacttt ggaagatgta catcgcccaa
    26281 gcaccaatga cagctggaac accggtcaac gccgtcccga tcccatgcaa aatcgtattc
    26341 aaagctgaag gaacagatgc atcagatcct cccaagacaa gaagaatccc tttggccata
    26401 cctactacga ttgctgtacc agctaaatca gctgcaccag attggaaaga agaagcgatg
    26461 tcattgactt tcattccatc taatttgaaa agaacagcaa tgattccagc aactaatccc
    26521 atcacaaaga attgagaagc gatttctgga atgtaatagc ctttttgagt cacgccccaa
    26581 acgatccaga caaggacaac taacatctct aataagatca atttatgccc aagcatgaat
    26641 tttttatttt catcagatgt ctttttgata tgagaacgga aataatcatc tgatttgtaa
    26701 gtcaacgaac tttctggttt tttacggatt ttttctgcat atatcatcat ataaccagcg
    26761 gctaatgcag taacaactac ccacataatc aatcggaatg tcgcaccaga caacacgggg
    26821 attccagcaa tcccttgagc caccgctacg ctaaacgggc tcatccaaga ggtcgcattt
    26881 ccgacttgag atgcgacata agtcacggtt actgctacga tcgagtcata tcctaacgca
    26941 atgacaaatg gaaccatcac cattgagaat ggaattacct cttctgccat cccgaatgta
    27001 gcgccaccga atgaaaaagc aaagaataat agcggtaaag ctaaacgctc cagtccctta
    27061 gttttactga taaacgcata gatacctgcg tcgatcgctc cggtacgcat aataatacca
    27121 aaagcacctc caacaacaag gatcaatgca gcgatcccta cagcagatcc atacttatct
    27181 ccagatacta gtccttcaaa aacaaaattt aagaaaccaa agccgccaaa atcatctgtt
    27241 ccccatattt tggctgtttt atgcaatttt ttagaagtat catagatatt ttcgccgtac
    27301 tgatcgtaaa gcacatcatc tgtcaaggag atttcatcta gtttttcctg cgtaagattt
    27361 ttttctccgt cagcaatgac tttttccaat cctttttccg gaacatccag tttttcacgt
    27421 tctgctggat gatcttgcag ttcttctaat tgatcaaaca catacgactt atccagctcg
    27481 taagcataac ggaaggaatc ttgacgtaaa acagtacgag tacttgtttc gccatttgca
    27541 tctttgtatt caatatcttg cgtgctgaac tttcctgctg gaacgacaaa agtcagtatc
    27601 catgcaaaga tgactacacc aaaaataatg acatacgtat ggggtgtcga taatttagtg
    27661 aagtcgcgtt tcggttttcc tgaagaattt gttttcttca aatttatttc ttcctttctt
    27721 ttttcagtaa ttttgatgaa taaaaaatgg ataatcaaat gaattataca gtaaaaatga
    27781 catttccaca tgaataattt attagaaaat cacttttttc tattttgttt ttttctccta
    27841 tgtatgatta tttttttctc tttccattta tattaagtat tttatttctc tgtttttcct
    27901 taaaattctt tttttatttt tagattagag aaaatctcgt cctgtttttt tcattttaca
    27961 atcattgaaa aattgaattt cattgcaaaa aaaataataa acacttataa atatccatta
    28021 tctatccttc aatttttgtg tattttattt ctattagaaa aaggtatcct gttggacaat
    28081 tttgttatat caggtaaact tcattgcaat aatgataaca aatgtactat aattactttg
    28141 taaagagttt tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat
    28201 atggattctt tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt
    28261 ataactcatt tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca
    28321 agcttaaaat caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt
    28381 aatcctcatt aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat
    28441 ttttttaagg aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc
    28501 tgttttgctg taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt
    28561 ccgcagatcg ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg
    28621 ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg
    28681 atacttcgtg acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc
    28741 cactgagcta gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg
    28801 tcttctgatt tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca
    28861 tagacacctc cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc
    28921 caaaattcaa atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct
    28981 tgattaattt gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt
    29041 aatttctctt ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc
    29101 ctagctatac ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac
    29161 agtagttaga cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac
    29221 gaatacgccc aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct
    29281 acataggggt agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat
    29341 tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt
    29401 agcgccaact aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg
    29461 taagtaatct cttatccaag tgcaagcaag aaaatacaaa aaaacattgg tatgatttat
    29521 atatatataa ataacataaa aaaaggaatg gcttatcccg atacaacaag cattccttta
    29581 aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat aaatctctca
    29641 tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa tattaccgtc
    29701 ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta gttgtatgtc
    29761 taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg attagataca
    29821 gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc aattcaacca
    29881 tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac gtttcttttt
    29941 ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac acaaatatcc
    30001 aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca acagcgacta
    30061 attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct ttttattcta
    30121 ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt ttatacaact
    30181 taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa agtctttttc
    30241 tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac gacagttgcg
    30301 caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca gcaggccact
    30361 tatttccagt taactttctg ttcttagctt tttactcaac tggataatat atgcacttaa
    30421 tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca tatatcctaa
    30481 cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg cctctgtctg
    30541 gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg gttcttgagt
    30601 ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg ctaaattact
    30661 tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac gacccttttc
    30721 gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg gtacacgcat
    30781 gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt gagtcaatgg
    30841 gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat ttcctataaa
    30901 tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac ggatgaagtg
    30961 aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt ccttttcccg
    31021 caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc ttttttcttt
    31081 tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca caataaactg
    31141 gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg ttgccggcaa
    31201 aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca cttgctgttc
    31261 ctcctaaacg acgattaact attgatcatc tatttaacga agagaaaatt cattctccat
    31321 ttttccgtca cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa
    31381 aacattatag attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac
    31441 tgctgctttt gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc
    31501 cagcttatgt ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt
    31561 acaagtaacg atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc
    31621 catcgcgaca ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc
    31681 atcatctccg aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag
    31741 tgctgcacca gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt
    31801 catgaacagt ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt
    31861 tgtgatatca tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc
    31921 ctgtcttttt tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc
    31981 ttgttctagt cctttacttg catgtataat gattggctta gtattcattt taccaataag
    32041 ttcctgtgcg actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt
    32101 atccactgct tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg
    32161 taaaaaatgc tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc
    32221 ccaaatacgg acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga
    32281 tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata aataattgat
    32341 aatttccatt atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt
    32401 ttgtttactt cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac
    32461 attttatttt tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg
    32521 ttactaccag cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa
    32581 atacataaga gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt
    32641 ctctgttgac ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct
    32701 atgtaaactg gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa
    32761 atagtactat tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata
    32821 agttgaactg tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta
    32881 aaaaaataaa gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt
    32941 ctatcctaat aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac
    33001 tgctccacat gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca
    33061 cgtccgcgat atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta
    33121 taattgctgc aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca
    33181 tcttcgccta aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga
    33241 agaaactcgg cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac
    33301 atggcatatg aataaacagc tccccattga ttccctgctc caataaaata atgcatacta
    33361 tcacgtcgat gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc
    33421 actaaccctg ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat
    33481 tctccattca aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca
    33541 ggagctggat aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg
    33601 ataaatccaa tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct
    33661 tgagttgtac cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt
    33721 ccagtagtgt aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc
    33781 atataagtga cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca
    33841 gcgtcaaatt tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct
    33901 agtgcgcctt ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc
    33961 tcttataatt tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca
    34021 atacctttag ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat
    34081 acagacagat attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt
    34141 tttgtggtaa tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc
    34201 gaacgttcct tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc
    34261 tccaggtatg ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt
    34321 gtttcagtca atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg
    34381 gtgacgaaac gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt
    34441 ttttgaataa tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt
    34501 tctatttttt tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac
    34561 tgtaaaacag tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct
    34621 aaatcaataa tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca
    34681 acaacaggta acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc
    34741 ccgctttgtg acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa
    34801 aaataaaaag gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc
    34861 gctgctaaac aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt
    34921 ttcatatgtt ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt
    34981 tcttcataga tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc
    35041 tatccacgaa ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac
    35101 ttctttcttc aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac
    35161 gatatcatca aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg
    35221 aatattgatg gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga
    35281 cgcgcctaat gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt
    35341 tcctaagcga tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa
    35401 aatcaaaatg aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac
    35461 acctgtggca atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg
    35521 tcccatcaaa gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt
    35581 attttcttcc tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt
    35641 gttaaaaaac tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa
    35701 ctttttcaaa atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt
    35761 ccaagaaaac tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat
    35821 tttgctgttt ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa
    35881 gtggtagttg acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa
    35941 tagctactcc ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat
    36001 atggtgcaac ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa
    36061 tcaaccctgt agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat
    36121 ttccgatcgt attgcttccc accatataag aaaccattac cacaacacca agcatcaagg
    36181 aaagaccgat accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg
    36241 cataattgtc tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca
    36301 atatcaatgt caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc
    36361 cagctgtaat agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg
    36421 aagcacctac tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc
    36481 cagtttttac atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa
    36541 gcaacgatgt ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga
    36601 ctgcttgtac aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt
    36661 ttatctgctg gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag
    36721 cgttcctcct cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt
    36781 gccttgatcc ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat
    36841 gcatcctcga tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc
    36901 aacgcggcaa tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct
    36961 aatttgatcg ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata
    37021 cagtctgcgc ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg
    37081 tcgactcttg tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct
    37141 gattgagccg ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga
    37201 tcaaaacagt tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca
    37261 aattcaatcc ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt
    37321 tgacacttcc gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa
    37381 aaagctgcct tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag
    37441 taatagaatg atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt
    37501 ttccatggga agctaagata atttctctat ccactttttc atctcctctt ttcacttaca
    37561 catactaaaa aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa
    37621 ttcttcttct atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat
    37681 ctcagaattg gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat
    37741 agcagataac ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat
    37801 tggcatttgt cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt
    37861 tttgggattg ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat
    37921 ttctcccatc accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga
    37981 aaaattacga atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc
    38041 caaccattca gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata
    38101 gtcgatattg gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga
    38161 aaataatggg tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt
    38221 caactgacaa gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc
    38281 gattccagtg aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag
    38341 tcgccattct ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt
    38401 tttttgcagg cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa
    38461 aagcggtacg gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc
    38521 tactaatatg atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga
    38581 agcaatctgc tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg
    38641 taataaacga tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat
    38701 catcgctttt ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag
    38761 taaaatccga tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat
    38821 tcgttggaca tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt
    38881 gattagttgg ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag
    38941 atagtaactc aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat
    39001 ttgtcgaaag gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg
    39061 tctactggtc tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc
    39121 ttcgcaagaa tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact
    39181 tttaacgatt cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc
    39241 ttgcgtaata tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc
    39301 tttttgcaaa tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc
    39361 ttttgctgct gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg
    39421 acttgttaaa agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg
    39481 ttcttcgaga aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag
    39541 tgcaggtagt tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt
    39601 aatctcttca gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc
    39661 tcccatacga taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg
    39721 tgttaagcga tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc
    39781 ttttttattt tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt
    39841 caaaagttca ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc
    39901 ttctagcaga tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc
    39961 tgtgccactt tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt
    40021 tttgatttgt tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa
    40081 gtcttttggt tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa
    40141 attcgtgcgt aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg
    40201 tcgagaattg attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac
    40261 tgtgtttctt tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat
    40321 atgttctaaa tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa
    40381 caacttattc acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta
    40441 ataaagaatt gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt
    40501 tattctaaaa aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga
    40561 ataaggaggc tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc
    40621 ctggaaaact tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt
    40681 gctttatatg gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc
    40741 cgaaacagga tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac
    40801 aaaataagat tcacagattt caactttcct gtctgttttt agctattaca aatccttata
    40861 tgtggtataa caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag
    40921 agtaaagagg tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt
    40981 ttcccattga ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt
    41041 tattttcaga tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc
    41101 catcaaacct gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct
    41161 aagatagtac ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa
    41221 aatcaattag atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc
    41281 aagaacaaat taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt
    41341 ttttatgcag aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga
    41401 gactggcatg ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag
    41461 cttcttcata ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc
    41521 ctaagctaca atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga
    41581 atcatatgat tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc
    41641 cgttcttcaa atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct
    41701 tgatcgataa tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga
    41761 tggtcgctat gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag
    41821 tagcttcaca aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt
    41881 tctctcacaa cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg
    41941 acatcaatca gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt
    42001 ttttaagtat cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt
    42061 tcttagcatt attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc
    42121 ctctctacca aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc
    42181 ttagcggtgt tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg
    42241 ataaaagcac aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat
    42301 tatcacctgc tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg
    42361 ggatcaaagt aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata
    42421 tgaatcaaat gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg
    42481 ccgaagcttc aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag
    42541 ctgaggtcag tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg
    42601 acttccgtta tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc
    42661 gaaatacagt ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat
    42721 tgattccgcg tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc
    42781 gttcttggca gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac
    42841 tatttaccgg gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag
    42901 aatgttggga agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg
    42961 gtttagatac aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac
    43021 tgacgattgc aagagctttg attgccaagc cagcattact gattttagat gactctttaa
    43081 gtgcgttgga ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca
    43141 gtacagtgat tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag
    43201 tgatggatca aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag
    43261 cagaatatca ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt
    43321 atccttgcag acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg
    43381 gctagctgtg atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat
    43441 tggtcaaagt atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca
    43501 tttacttatt ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca
    43561 agtgttaagc aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc
    43621 tcatctgaat cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag
    43681 tcgatttaca aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt
    43741 attttctgga gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact
    43801 tctgacattg gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc
    43861 tcgtgcttca caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt
    43921 tgtgaccgaa cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag
    43981 tcaacagcaa tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt
    44041 ttcttcttcc ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat
    44101 tggtttagtt ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat
    44161 ttccagcttt acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat
    44221 cacgacacaa ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca
    44281 gccagaagaa aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg
    44341 aaaagtctct tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga
    44401 tttcaatttt actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg
    44461 aaaatccaca cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac
    44521 agtcgatgac catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat
    44581 ggtgttacaa gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa
    44641 tccggaagca agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt
    44701 tgtagaacgt ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc
    44761 agatggacaa cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt
    44821 gatcttagat gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc
    44881 cttcttagca atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat
    44941 caaaaatgcg gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca
    45001 tgatgagctg cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc
    45061 acaataaaaa caagcagtct tctgggatgg gaagactgct tgttttt
//
LOCUS       DACIWG010000022        44622 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.022, whole genome shotgun sequence.
ACCESSION   DACIWG010000022 DACIWG010000000
VERSION     DACIWG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.022"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(265..735)
                     /locus_tag="IYF54_001214"
     CDS             complement(265..735)
                     /locus_tag="IYF54_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ9831556.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1065..2705
                     /locus_tag="IYF54_001215"
     CDS             1065..2705
                     /locus_tag="IYF54_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ9831557.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2859..4181
                     /locus_tag="IYF54_001216"
     CDS             2859..4181
                     /locus_tag="IYF54_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ9831558.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4249..4683)
                     /locus_tag="IYF54_001217"
     CDS             complement(4249..4683)
                     /locus_tag="IYF54_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831559.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4748..5584)
                     /locus_tag="IYF54_001218"
     CDS             complement(4748..5584)
                     /locus_tag="IYF54_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ9831560.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6326..6952)
                     /locus_tag="IYF54_001219"
     CDS             complement(6326..6952)
                     /locus_tag="IYF54_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831561.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7247..7582)
                     /locus_tag="IYF54_001220"
     CDS             complement(7247..7582)
                     /locus_tag="IYF54_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ9831562.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7569..7853)
                     /locus_tag="IYF54_001221"
     CDS             complement(7569..7853)
                     /locus_tag="IYF54_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ9831563.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7909..9432)
                     /locus_tag="IYF54_001222"
     CDS             complement(7909..9432)
                     /locus_tag="IYF54_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ9831564.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9425..10600)
                     /locus_tag="IYF54_001223"
     CDS             complement(9425..10600)
                     /locus_tag="IYF54_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ9831565.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10604..10789)
                     /locus_tag="IYF54_001224"
     CDS             complement(10604..10789)
                     /locus_tag="IYF54_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831566.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10755..12449)
                     /locus_tag="IYF54_001225"
     CDS             complement(10755..12449)
                     /locus_tag="IYF54_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ9831567.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12446..12919)
                     /locus_tag="IYF54_001226"
     CDS             complement(12446..12919)
                     /locus_tag="IYF54_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ9831568.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12988..13143)
                     /locus_tag="IYF54_001227"
     CDS             complement(12988..13143)
                     /locus_tag="IYF54_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ9831569.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13277..13657)
                     /locus_tag="IYF54_001228"
     CDS             complement(13277..13657)
                     /locus_tag="IYF54_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ9831570.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13661..13876)
                     /locus_tag="IYF54_001229"
     CDS             complement(13661..13876)
                     /locus_tag="IYF54_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831571.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13879..14286)
                     /locus_tag="IYF54_001230"
     CDS             complement(13879..14286)
                     /locus_tag="IYF54_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ9831572.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14552..16027)
                     /locus_tag="IYF54_001231"
     CDS             complement(14552..16027)
                     /locus_tag="IYF54_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ9831573.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(16017..16865)
                     /locus_tag="IYF54_001232"
     CDS             complement(16017..16865)
                     /locus_tag="IYF54_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ9831574.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16902..17264)
                     /locus_tag="IYF54_001233"
     CDS             complement(16902..17264)
                     /locus_tag="IYF54_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831575.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17564..18188)
                     /locus_tag="IYF54_001234"
                     /pseudo
     CDS             complement(17564..18188)
                     /locus_tag="IYF54_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18185..18496)
                     /locus_tag="IYF54_001235"
     CDS             complement(18185..18496)
                     /locus_tag="IYF54_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831576.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18540..18833)
                     /locus_tag="IYF54_001236"
     CDS             complement(18540..18833)
                     /locus_tag="IYF54_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831577.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            19027..19674
                     /locus_tag="IYF54_001237"
     CDS             19027..19674
                     /locus_tag="IYF54_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9831578.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19735..20880
                     /locus_tag="IYF54_001238"
     CDS             19735..20880
                     /locus_tag="IYF54_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9831579.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20961..21266)
                     /locus_tag="IYF54_001239"
     CDS             complement(20961..21266)
                     /locus_tag="IYF54_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ9831580.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21291..21722)
                     /gene="ruvX"
                     /locus_tag="IYF54_001240"
     CDS             complement(21291..21722)
                     /gene="ruvX"
                     /locus_tag="IYF54_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ9831581.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21719..21985)
                     /locus_tag="IYF54_001241"
     CDS             complement(21719..21985)
                     /locus_tag="IYF54_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ9831582.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22240..22965)
                     /locus_tag="IYF54_001242"
     CDS             complement(22240..22965)
                     /locus_tag="IYF54_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ9831583.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23326..23396)
                     /locus_tag="IYF54_001243"
     tRNA            complement(23326..23396)
                     /locus_tag="IYF54_001243"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23362..23364),aa:Gly,seq:ccc)
     gene            23513..24265
                     /locus_tag="IYF54_001244"
     CDS             23513..24265
                     /locus_tag="IYF54_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ9831584.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24281..24760
                     /locus_tag="IYF54_001245"
     CDS             24281..24760
                     /locus_tag="IYF54_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ9831585.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(25011..26591)
                     /locus_tag="IYF54_001246"
     CDS             complement(25011..26591)
                     /locus_tag="IYF54_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ9831586.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26584..27327)
                     /locus_tag="IYF54_001247"
     CDS             complement(26584..27327)
                     /locus_tag="IYF54_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831587.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27503..28279)
                     /locus_tag="IYF54_001248"
     CDS             complement(27503..28279)
                     /locus_tag="IYF54_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831588.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28527..29363)
                     /locus_tag="IYF54_001249"
     CDS             complement(28527..29363)
                     /locus_tag="IYF54_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ9831589.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29374..30303)
                     /locus_tag="IYF54_001250"
     CDS             complement(29374..30303)
                     /locus_tag="IYF54_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ9831590.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30495..30848
                     /locus_tag="IYF54_001251"
     CDS             30495..30848
                     /locus_tag="IYF54_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ9831591.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30922..31578)
                     /locus_tag="IYF54_001252"
     CDS             complement(30922..31578)
                     /locus_tag="IYF54_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ9831592.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31804..33738
                     /locus_tag="IYF54_001253"
     CDS             31804..33738
                     /locus_tag="IYF54_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9831593.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33824..35047)
                     /locus_tag="IYF54_001254"
     CDS             complement(33824..35047)
                     /locus_tag="IYF54_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ9831594.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(35041..36939)
                     /locus_tag="IYF54_001255"
     CDS             complement(35041..36939)
                     /locus_tag="IYF54_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ9831595.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(36933..38051)
                     /locus_tag="IYF54_001256"
     CDS             complement(36933..38051)
                     /locus_tag="IYF54_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ9831596.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38134..38967)
                     /locus_tag="IYF54_001257"
     CDS             complement(38134..38967)
                     /locus_tag="IYF54_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9831597.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38960..39876)
                     /locus_tag="IYF54_001258"
                     /pseudo
     CDS             complement(38960..39876)
                     /locus_tag="IYF54_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(40103..41227)
                     /locus_tag="IYF54_001259"
     CDS             complement(40103..41227)
                     /locus_tag="IYF54_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9831598.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            41410..42318
                     /locus_tag="IYF54_001260"
     CDS             41410..42318
                     /locus_tag="IYF54_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9831599.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            42459..44072
                     /locus_tag="IYF54_001261"
     CDS             42459..44072
                     /locus_tag="IYF54_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ9831600.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13487 a         8892 c         7441 g        14802 t
ORIGIN      
        1 tattagctca aaaaatggca aaatatgagg aactattttg ccatttgtct ctctaatact
       61 caggtcagaa cgttgacttc acagtctctt ccttaaacgg tgttcaaaaa cttgcatctg
      121 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
      181 tacttgatgc agggcaggtt tttcacgacc tctgattcac ggtgtttctt tttcatcaac
      241 atttttacga aatactgtgg aagactattg aaataagtgt ggattgtttc tttgtgtttc
      301 aatcgtttct tgccattctt catttaattt tggattaccg ttttcttcct tgaactcata
      361 aggtcctaat tcaaaaatcc cagacagtat ttgattcacg atttcatata gttcagtgtg
      421 atcagacaca tgattcttga agatcatctc attatcacca cgtttgaacc tcacattcgg
      481 gctctcatga tgaacctcta tgcatttttc tccttttttt aactgacgga cttctgaaaa
      541 catctcataa atatcttctc cagtaaaacc ttcagcaagc gccagactgt ccacaagacc
      601 gcctactgtc agttcagaga ttattgcttg attggaatct aattttaatg tatagcttgt
      661 cgcttctact acgccacatg cattgcaacc gccttctgat agttcgcttt ttctaatcaa
      721 ttgaacttct ttcattttct cttcctcctc ttaattttct acttatgtat ttttatcacc
      781 tgacataggt gactcctatc aacgctatca tgtgcttttt tcctcagaaa tcctatttat
      841 ccattccatc tatcccttat tcttctactt ttctttctct agcatataag agatacgcat
      901 agtattcaag caaggattgt cgatataaaa agaacaaata ctgctatttt gatttctttt
      961 ggctatatta catataaata aaatttatca tacctgctct ttttgactat atcatcatct
     1021 gacttctata cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggttaa
     1081 acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa
     1141 aatatggaat cactggtatg cttccaagta ctgtccaaac actggaacaa caatctgttc
     1201 aagcttatgg tcaatactta agcaaacaaa cagatttgga aaaacgtatc ttcttaatga
     1261 atctattcaa tacgaatcgg acattgtttt ataaattgat gggcgaacat ttagttgaat
     1321 tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt
     1381 tcttgaaacc gcaagatgct gcatttcttt caatcgacga accagaaaac atcaaagcaa
     1441 gtttgaagaa cgcggcagat ggacgagaca ttcgtttgat tgttgtgact gatgcagaag
     1501 gaatcttagg tatgggcgac tggggtgtga acggtgttga catcgctatc ggcaaattga
     1561 tggtctatac tgctgctgct ggtattaatc cagcacaagt tttgcctgta tcgatcgacg
     1621 ctggtactaa taatgaagaa ttattgaata atcccttata cttaggcaat cgtcatgctc
     1681 gtgtagaagg cgaaacttac tatgaattca tcgatcaatt cgttcaatca gctaccgaat
     1741 tattcccaga attgttattg cattgggaag attttggacg aggaaatgct gcagcgatct
     1801 tagaaaaata cgaagatcaa atcactactt tcaacgacga tatccaaggt acaggaattg
     1861 ttgtcctagc aggtgtatta ggcggattga atatatccgg tgaacaattg aaagatcaaa
     1921 taattttgac atttggtgct ggtactgctg gtgtgggtat cgcaaacatc cttttagatg
     1981 agatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata
     2041 aacaaggttt attgtttgaa gatacagaag gattgactcc tggtcaaatc ccattcgctc
     2101 gcaaacgctc agatttcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag
     2161 cgatccatcc gacaatcatg attggtactt ctacccaacc tggagcattc actgaagaaa
     2221 tcgtgaaaga aatggctgct catacaccac gtccgatcat catgcctttg tctaatccaa
     2281 caaaattggc agaagcaaaa gcaaaagatt taatagaatg gacggacgga aaagcattag
     2341 ttggaacagg tatccctgct gctgatgttg aatataatgg tgtaacttac caaatcggtc
     2401 aagcaaataa tgctttgatg tacccaggtt taggacttgg tttgattgct tcaacagcta
     2461 cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg
     2521 taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttttctcaaa
     2581 cgattgctga aactgtcgca aaatcagttg tcgcacaaaa cttgaatcgt gaagaaatca
     2641 cggatatcaa agaagcagtt gaatctgcta aatgggttcc tgaatataaa tctttagaag
     2701 attaagatcg ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa
     2761 agcgactgcg gcaaaacagc ggccagccaa ctcctgctct tttgtcgctt ttctttggct
     2821 tttttcatat gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaactg
     2881 caactgcagt tgaggaaaag aaaaaagaag gtttttggtc cacaaaaatc accggcgtaa
     2941 gtttgccact ttatttgatc atggtccttg tcctgatcat tgcaatggct ttagggaaat
     3001 taccgacgaa tatgattgga ccaatcgcag ttttggttat tttcggtaat ttatttcatt
     3061 ttatcgggac aaaaatccct atcgtcaaaa gttatttggg tggtggttcc gtttttgcga
     3121 tcttcgcttc tgctgcgatt gctacatttg ggatcttacc tgaatatgta gtgaaatcca
     3181 cagaaaactt tgtcagcaat atgggctttt gggactttta tattgctgcc ttgattactg
     3241 gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg
     3301 ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtgggc atgcttatcg
     3361 gaaatggttt tgcaaattcc gttttataca tctcattacc tgcgatggct ggtggtgttg
     3421 gagccggagc cgttccttta tcaacgattt atgcaaatgt tttaggtacg gatgcttctt
     3481 ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcttagct atcatcggtg
     3541 cagcattagt tgctcgtgct ggacaatcaa tgccaaaatt caacggcaat ggtaaattaa
     3601 tggaaaaagg cgatcttggc gatggaaaac ctcgaactgt gaaaccttcc attccacagt
     3661 taggtgttgg tttgatggta tcattgactt tcttcatctt aggacaaatt ggtaactatt
     3721 ttgttcctaa agtccatgct tacgcattta tgattattat cgttgtcatc tgtaaaatcg
     3781 ccaacctatt accagagtat tatgaagatg cagctatcat gttcaataac ttgattgtca
     3841 aaaacttgac agcagctgtt cttgccggta tcggtattgc gttgctgaat ctgaatgtat
     3901 tagcttctgc tttgacatgg caatttgttg tcttatgtct gacaagtgtt atcgcgattt
     3961 cagttgtttc aggatttgtc ggtcgtttgt ttggtttata tccaattgaa tcaactatta
     4021 ctgctggctt atgcaacaac tcgatgggcg gaacaggaaa tgtagccgtc ttatctgcag
     4081 ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcgacttggt ggtgccatcg
     4141 ttttgatcat ctctggtttc ttgatgcaat tactttctta gtcaaaaaaa caaaaatacc
     4201 tgatcatcaa gtatcggctt ggtgatcggg tatttttttg ataaagattt acaggatttt
     4261 caaaaatctt ttttgcattt tatcggaatg ataaccgatg tgttcataaa attggtgtgc
     4321 ttctactctg tattctgcag aattaagcct tattgtcgtt attcctcgtt ttttcgcttc
     4381 ttcttctacg ccagtcatca aagcctttcc gatgcctctt ttttcagcat atttagacac
     4441 agcaagtgcc ataatattga acattggatc gctaaaaatc gattcatagg actccgcttg
     4501 tacatagcca gctgcttttt tagaagtagg atcttcataa accaagaaaa aatttttctc
     4561 tggctccttc agcagtttct tcatttgtct caccgtaacg gttaatggta tatcataccc
     4621 taattgatcc gcattgattt cacgtagtgc ttctgcatct tctaaacgca tttcccgtaa
     4681 catccaaatc accagccaat ttctgatttt ttaaaatgaa tttttttatc atagtatact
     4741 ctttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt ctagattctc
     4801 ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc acgaggacaa
     4861 ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4921 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4981 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     5041 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     5101 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     5161 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     5221 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     5281 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     5341 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     5401 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     5461 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     5521 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     5581 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     5641 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     5701 gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
     5761 acaaaaatag ctcctcataa ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
     5821 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
     5881 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc
     5941 aggagaacac cgtttaagga agagactgtg aagtcaacgt tttgacctga ttattagaga
     6001 aacaaatggc aaaatagttc ttcatatttc gccgtttttc gagcgaatgc cgaggggcag
     6061 ctgattgaac atcgtttatt tggaaaaagg gattgtgaca agacgtttgt cacaatccct
     6121 ttttttatat cttattaatt cattcttttg ttctattctt cttcttccgc catgaatgag
     6181 aaacccatat caaagaacta taaaggttat caaaacagcg tttatgacta aatgaattag
     6241 agttatctat cactattttt caagcgattt aaaccaaatg gcatacaata tggcatacaa
     6301 aaacttcaat aagccgtggg ggtttttaaa tttcttcttg ttctttaaaa tcaatgagtg
     6361 gagctacata atttgaatcc actttttcaa acgtcacttt ttgcatcgac gaatgttttc
     6421 tttcccaaga taacgtaaat agatttgact ctggacttat aaagtctact tcaccttcta
     6481 gtattacttt cttatgcaaa tattcctcta ataaatatag acttgctaaa gcatacaaca
     6541 cattttccaa atttgcttcc ttataatttg aagaacgacc atgttttact ctgttatata
     6601 ttttccacca atctggtgta tcttcaggcc acccatctcc aaaaggtttt agtgaagtca
     6661 atgttaaaga attcataacc ctaacaccat tatccaaaca cattaattcg ctagatttaa
     6721 taacagcttt aaaatcagtt atgcttttat tgctactgct atcaaatccg caaagttctt
     6781 tgaaaataat ctcacactcc gaaccaatcg ctagtagtag tttttgatat tcaatagaga
     6841 atgttttgaa attattctca tcgagcatca caaaatctgt ggtattaata aactcatttt
     6901 ctaaatgtag ataatacttc caataaaaat ttgtaaactc tgctttgtcc attaaatcac
     6961 ctcttttgaa ttataacaaa aaaaacacgt ccacaacaga cgtgttcttt gatttctaca
     7021 caatcagctt gccaaaacca tccaatcagt cctagcatgc ctgtattaaa attgaacgtt
     7081 catcatctat catctttctt agtccagcat gtttgcaacc acacctacaa ttttcagtcc
     7141 aacaatagat ttcgactagc taagtcttgc aaaattcagg cagctaaccc ttcatttact
     7201 accttaatta tactaaatac ccacaacgtt tacaagtgta atcattctaa gaaatctgtt
     7261 tagctatgat cgttgtataa tctttaaacg ccgttccttc attgattgaa ataattttat
     7321 aactcttgtt tttccaagct actttcatat cgtttgttag ttcataatct tgatcgtatc
     7381 gaataataaa cgtcaacgtt ccttcaagaa tggtacctac actagcaagc acgtctttta
     7441 gcatttgtga acgcacatta gcccaacatt caaatacagt ttcttcacta tccaccatct
     7501 gcccatcttc atcttttacc cgttttgtct ttttaaactt gactctctca gtcaattcat
     7561 ttacattctt aatgattggc ataataagca cctctcattt gttgtactaa tgcttgtacc
     7621 gttactggta tccgttcgct gctggcttct tgacggttca aataccaatg ttgagtaagt
     7681 aatgacaccg ccaaatcaaa ttgctgataa ttatccatat ctttatccgt tgcgctacta
     7741 tcaatagcac tttttatata ttcgcctgct gtatcaatca attgttgaat aaagtcatca
     7801 tcaatagtgt gatcgatccg catacttttt tttatgctat ccaaaattac cattttatct
     7861 ctcctaatga aaaaaaggca gtgggataaa ccgccgccca atatttttct atacttttgg
     7921 tgttacaact ggcgtaaact caatatacac tgaagcattt tgatcaattt tcttataatc
     7981 gttgcgaacg atcactgcaa gtccttgtga gtagtaatcg aatttttccc attgagtcgt
     8041 tacttgattt ctacgagcaa caaagataga ttgtgcaata tcccccataa tcatagggaa
     8101 tgttccagct ttaggattgg caaacaatgt atctgcaatc aatactactg gcataccgaa
     8161 taatgattta ccactaggtg ctgtcacatc gggttgtaaa atgtaacgtc catctgaatc
     8221 tttcaatgta tctaggtggt aatatccgct ttggtttaga attaccattt tatttaatgc
     8281 tgggtctaat gccacattgt atagttgttt caaatcgtcc aacgtagcag ccgttttctt
     8341 agtgaatgtt tttaataaat ccatgatatg cttattgtca gtgttttcta ccaatttcgc
     8401 taactgatct ttgacctctt gtacaatatt cactgctgaa tcttccacaa cctcatttga
     8461 taaggcaatc ttaccagcac gagtttctac tttataatca actgaagtaa acatttcagc
     8521 gtcaatatca ccaatttcag caagttcagc ttttgttgct aacactgctt gttggttagt
     8581 tgctactgga tatttacctt gtccatttga tactgttttt actgttgcat attgagctaa
     8641 gttataattt gaacgtttca aatcaaagac ttcaccgatt acttcttcgg gaacaactgc
     8701 cgctgcattt acagtagtga ctccatcacg cacttcgcct tgtgaacgga tatattcttc
     8761 atacccacgc aattctgttt tttcgttatc gattaaagtt ttttccatgt tattactctc
     8821 cttttgtgct ttagaagtgg attctaagct attgtttgtt tgttttgctt ggttgttact
     8881 cataaacgat tcgtaagaac gcttatttac ttggacattt gatgaatcat aagcgggaac
     8941 ggtcacgaca ctaatttcat taagtgcttt gattttatct attgatcgtt caattgtgcc
     9001 atcttctttt ttgtcgaatg aatctaatcc aagcacgaac ccaaaggaca tagaatcaac
     9061 aacccctttt gagatattct catacacatc atttgaatag gtcgtgtcgg ttagctctgc
     9121 ttcaaaatgt aatcctgtat catcgacatt caatttcaac gtacctgctt tgacactagc
     9181 tagtggcttt gaataatcgt gtccatataa taagaacaca tttgataaat ccacctcttt
     9241 taatgcttcg ggtgtaatca cttctacaaa gccgcctaaa tcttttgacg gttgcccgaa
     9301 tttcaacgca tagcctgaaa tagttttgcc gtctgttggt tgtttttcat tttctttctc
     9361 tactttggga gaatcggctg ttagctcagc ttcttctgtc agccgttttt ccttttcttc
     9421 ttcattattc aactggtgta actccttcct gttcttttaa ttgatatttc tccaacgtat
     9481 ctaaatacgt aaagttcaaa cttgctagta aacgatcgcc accatccatg gggggtagcc
     9541 ccattttcga ccgcccctca ttaatcgtga gaagtgaccc tttgacctga tccaatacat
     9601 tttttacttt tgtttcagga tcagtctcta gtaactgatc ggaattaaag cggatattgg
     9661 tttcaagttt cgtatctaat tcactcacaa acacattaaa gtaatgaatc agcgtacttt
     9721 ggatatactg gagattactt tgaacggtgc tagaatgttc attttcaacg cctaaacgct
     9781 caatcggtac accaaacgcc ttggcaattt gttttgtgtt ccaatcattg gaattgataa
     9841 gcttcaaaac atcggtgttt atttctaatg ttttgtagtc cattgtttca tcaagaatga
     9901 tcgttctaag agcattatct ccactacttg aaccgttggc ttcttcaaac ttttcacgaa
     9961 ttgcgttctt tgctgatccg tctaaatctg acttatgaac cttcaaaata cctgacccac
    10021 tgacaccacg agtaaagaaa ttgtgcaatg tgcggttgcc agcttgttga attttcagtt
    10081 catcacgtaa cgcataaagt ggtggtaatc ctgttaaacc atcttgggtg aaatatttga
    10141 agtgcaaaat atcgctagac ttcacacgtc tttttttatc accaatctga taagacaacg
    10201 taccattatc taattgagta accgttacac ttgagttagg taataagtga atttcttcta
    10261 ccttttcacc gttgcgttta atctctgcaa agctattacc gtttagcaac atattgaccg
    10321 ccaaagcaaa ctttaaatgc caaccatcca tttctgaatt aggtttctcg tttagtaact
    10381 tcaccagttc attatcagct tgtggcatat tgtttttgac caattgaatc ggacttgaag
    10441 caatatcact agcaataatt cgaacggctg caaacacatc actatttctt aatgcacgaa
    10501 cgcttgtaaa gctggtggtg taatcatccg attggattga taccacatgt tgtaggaaag
    10561 gatctcccgt tgtattttgt ctcgctttaa aaaatgccat cttttaacct cccttctgtt
    10621 ctgtatttag catgaccgct accccaataa gcactatgcc cagcgctaac aagccatagt
    10681 agacatttgt tagtaaggtg atagcaacca acacacacac caatccaaga agtaaaagaa
    10741 cggtatgaat gtaattagaa agaaaattct tcacttgtat aaaattcatt gtctgctttc
    10801 gctccttccg ttgtggtgaa gtaatccatt gcaaacacat aggcattgat taatgcagct
    10861 attggatcaa ttttgttact gttctttgat ttattaatct gaatgccgtt gttatccgtt
    10921 tttaaaatag cgttgttcac tgcatgggtg agaatcgtat ttttattgtg aataacgttg
    10981 ccttcataaa cttgttctct aaaagttcgg gtcggaacgt ttagagtaat cgttccttgt
    11041 cttacttcta acattgggta gttggctttt tcagctttac tgataagcga attagcgtta
    11101 tacgggtcat aacaaatccc catacattcc aaatcatttt cttgaatcaa atcttgaata
    11161 aactgaaata cttggtcata gtccacgatt ccactttcta attgagtaat ggaacactca
    11221 ccagcttttt ctagttctcg ataaggcaaa ccgtcgcgct tttctttgtc ttgaagccca
    11281 tatttggtgg ctacaaagct atgtgaatca caataaagtt cgccgttatc tagcggtacg
    11341 atccatgaaa cgctagttaa atcatctgtt ttagataaat ccactccgat ataaacgggc
    11401 ttgcctgtaa tatcttgtgg ttctacttcg gttgcctgcc agtcttcagc aatcatatag
    11461 ctgtcctcac tcgcttgtct ccataaattg aagtttttca ccaatacaga attaaggtta
    11521 ttttgtttca aagcaaggtt cacatcatct tgaatggttg gaatcattac ttttttgatt
    11581 tcttcacttt caaaaatcgg attggctttg atccatttct cttgatcgtg aatttcttcg
    11641 ggatcgtcca attcccatat cgctataaag tacctgtcgg cttgttcttc gccttttagc
    11701 acacgatcta acaataagta ttcctcgtac ataggaacgt ttaaatcaag cccagcggta
    11761 ctaataacac acaataagcc gttcttttgt tgggtcattc ctgacttaat gacgttgtac
    11821 gttttacgag tttttgcttc gtgccactcg tcaagaattg cggttgttgg tgcaaaccca
    11881 tctagcgtgc tggtttcact agccagtgcc atagcgaacg aattagaggg caagtgctga
    11941 atcttagagt tcataatttt cagttgtggt cttaaaaact tactagactt gaccacgttt
    12001 ctaagtgaat tagacagcat atcatagccc aatttagctt gtttcaaagc gttacttaca
    12061 aacaatactt gccttgcttc tgctggttct tgttccataa tcaacgcatt ggcagccatg
    12121 ccacttgcga tatacgtttt cccattctta cggctcatac tggtaaacgc tcgattgaat
    12181 cgcctgtaat tcccttcttt agtacgccag ccataaagac tgcctagaat gaatttttga
    12241 aatcctaaca tcttgattgc tttaccgtcc gtagatggta gcaactcaat gaatttaatc
    12301 gctttggtgg cttgttcttc atcgaacaca taagggaagc tatctgactt agaacgctcc
    12361 aaatcgctta tatgacgctc acacgcttgg ataattttct tgggtgcaat cagcttacct
    12421 gataggactt tctcaatgta atcattcatg aattcatcaa ctctttgaac gggtcttcgg
    12481 gttcatcatt tttaaccttg ttcaaagcta gttttgatcg tgattctaag gtcataccta
    12541 gtgaggtggc ttgttttttc aaatcactca tagcttgtga ttgaacccga accgctggat
    12601 tggctttctt tactccgttt tcgcatgtga taaacgtacc tgattttctt atctcggctt
    12661 cggctgtttt cattcgagca aaggctaaac aataggctac caatgaacca taatctgctt
    12721 cactcaatgg ataatctttt tttaatgttg gtacgatacg attccattca cttctagcgc
    12781 tcgctgccat ccagtcaggg ggcgtgatat ttattaatgg ttgctgcttg aacaattctt
    12841 ctctagcgtc cacacggatt gctttttctt cacttgatat attgcctttt gtatcgtgta
    12901 atattttagg gtgtcccatg ttgtcctcct ttcagacaaa agaaaaagga acgtaagcaa
    12961 ttaagctcac attccttcgt ttttcgatca gaatttttct ttatctaatt tatctatata
    13021 aatgattttt ccgttctgca tttgaatggt cacttgacca aaatgaggta actcaatggt
    13081 gcgctgtttc cctgttttat cagtaataac aattgtattt ttcaataaat ctcctacttt
    13141 cattatatac tttttcactt gtgattacaa gtgtaatcgt tatttgtaat tacccttatg
    13201 ccttttcaca acggttattt gtaccaaaat gagtaggcat cgattgtcag taagtttcac
    13261 actatgcccc cttaactcaa agggtactct tttctcgtct ttgtctttca ttggctgtct
    13321 tggaattatg gtgtgcatgg cataaagaac gaaaattagt ttcatctaac cgtctattcc
    13381 aatcatcttt tagttcaatt ttgtgatcta caacatcagc tttccttatc aagccttctt
    13441 ctaggcaatc ctcacatact ggatgattta accgatacaa gtatgaagcc tttcgccatg
    13501 ttctactttg atagaattga aaatatttac cttcgctttg ctttctttct tcacgcttaa
    13561 ttgccgtttg ctttgcttta tgcttcccac aatacttctg cctataatca atcaatgttt
    13621 tacatccagc atgattacat tgttttttaa ctgccataga ttaccaacca aagcgatctc
    13681 gttcgtcaga atcatcttgt tcccattttg atttgtattc aatataaggc gaaactatct
    13741 tcaagcacgt tccgtcttta aaattaactt tagtgatttt atcttcctca aaactaataa
    13801 agtctacatc ttcttgagtg tttgaatact caatgtgttt ctcaccatcg aagtatctaa
    13861 tactttccat atgcataatt attctacctc caataaatta gttatcaatt catcatctgt
    13921 tagtagcaaa gccaagaact tatcatggat gtttaaaaac aaattatcct cattcgatgt
    13981 aagctctcgt tgtctcgctc gatcatatag gttaagcaga caattaaatt catccattag
    14041 ctcacctgta tcatcatagt tgccactatc taccaatgat cccactacta gcgattgaca
    14101 ccacgctact gactcctgca gccaatcagg ttcttcttcc attatcttta atacttctgg
    14161 atcaaatgtg gaataatctt ctgttaccaa tcccacacta aatgccagcg tgttcattag
    14221 aaaccgatcc atttcatctg tatattgaaa gttcggtgct ttgtttgttg aaataatatt
    14281 gagcatttac tttcctcctt gattcgtaac acgtggtaac acctacgtaa caggtacccg
    14341 ttaccactca atcccaacgg ggacaagggt tctagcgttt tggtaacagg taacgggtaa
    14401 aatccagtaa cattattccc cagcatatta gctaactatt tcaaaagttt taaggaaata
    14461 aggtgttacc ccgttaccaa attgctgtat cccttggggc agtaagggta aactagtaac
    14521 gggctaggtg ttactgaccc gttactaccc gttacttttt gtactttcca gctcgatttt
    14581 tattttcata ggcatttgac ttattgtaaa atcctcgtcg tttatttctt tttggcagtt
    14641 gcttccgttc ccaaccagga atactgctaa taatcaaggc aatcttttta gtttctgcat
    14701 ctgatcgacc attcaataaa tcctttgctt gtttatcaaa aacagcttcc aaaatatcag
    14761 cggtgagtac gctatcaact aaataaaact cttgttgcat agtagctcca ttatagaaat
    14821 gctgtaaatt tttctcatta catttactgt caggatagtt ctgataataa gaacgtctcg
    14881 cccacgctgg agcttcttcc catttatacg ggatttccat ttccaaatat ttagtgattg
    14941 cttctttaat tggattctcc atcttggcat cctcttgata gtcttttgca atttctaaga
    15001 cttctttatc ttcatcggga ctaaaataga ttcgttgtcc tttatcaaaa agcacctttg
    15061 cttcagctaa cacttgaaga aaatattcat cctttgtttt aaacggattc atctttggct
    15121 tattcttaca aggcaaagga taaaaacggc ggtttcctgt atcatctttc aaatactgcc
    15181 catcattcga cgttccaata aatacacatt gccttgccca agctatgaca ttccgaccat
    15241 aaggaggtct aattttatcg atcttggcac tgatgaagtt ttttatctgt tcaattttag
    15301 ttttgttcat tgaagataat tctccaagtt caataattac attgccttga agctgcatat
    15361 aatcatcttt cttttcacct agcgaatcta atgaatcggt gaaaaaatca tccgtgtaaa
    15421 gtaatgctgg aacggtactc ttaccaatcc cttgcggtcc atccaaaact ggaacgatct
    15481 caaactttat tcctggttta tagatacgtg cgactgatcc gactatccaa cgttttgtta
    15541 cttctcgtgt gtaaaggttg tcgtctaccc ctaaaaggtc aataaacagt gtttccagtc
    15601 taggctgttg atcccattta actttttcga tacgttcttt tactgggttg aaaacattct
    15661 ttctagccac atttactact gctttgtgta ttttgttgtc atcaaaaact acattgtagt
    15721 tttcttcaat atatgccgtt aaggcatcct caaaactgtc ttcccattct ccagcggtac
    15781 gattgatcca aggagaatct tttaataggt gaatgtatcc gctaaattcg ttataagcaa
    15841 ttgttccagc taaattgcta tcattggtta aaatcaattc tgcattcaca acactgttac
    15901 ttttaatctg atcgtcttta ttgcatctta atcgtgacat ccaggttttc aattcaatga
    15961 tattgtcttc acgctcgctg gcttcgggaa agtctaaaat actatctaat ttatgcttat
    16021 gagccatgag ctttccccct caaatctttt ttaatcatcg atcgaataat ttgattcact
    16081 tctttatctt ttagcggtgg ttcattgtac gcatttgctg tataaatcaa ttctgctaaa
    16141 gtttgattat ctacaccgtg ccatagtaac caaccgcata acttagttaa atgatcgttt
    16201 ctactacctt cgccagcgcc ttgaacaatc atatctaatt tttttgctgt ccctgttttt
    16261 gctacgggtc ggtattgagt ctttatttct tgcggttttt ttagcagtag gttcactagc
    16321 caatcgggag ctggtgcaat attatttaga gatacgtttt caataggttc atatgctctc
    16381 ccaacacttc ttgaaataat catagttggc gctattggaa ctcctttagt gataatatca
    16441 atcccgctta aacttttacc tttatcatca cgcccgaaga tatcagcaaa tgcacctgtt
    16501 tcttgttgga taattacacc atcaggaatt ttaaagaaga aatggattcc tttacctggt
    16561 gtgatttccg tataagtaga aggaaatgga tcatacattg aagcaagttt tttataattc
    16621 tcaacgccgt ttcgtgtttc agaatgttga tctaggtcaa ctaccaccat ttgatgatct
    16681 tcaagcatta acccaatatt tctagttgca tcttccgtcc accattcatt aatttgatta
    16741 gaatcgttag ttgcatcatt ccaaccacta gagcctttta ttggaatttt agttccagga
    16801 gataagggat aaattgacca tcccgacttt tgtaattccg ttgctgcttg ataattattt
    16861 gccaataaac ctctcctcct ttttcaaaat actggtataa atcattcaga cacctccatg
    16921 agcttcaact gctcaagttc gtatttcaac ccatcccgaa caaattcagc accttttaat
    16981 tgattctgat actttaccca tgtattaaat gttttcaccc ttgcttcttg catatcagta
    17041 atgtttaacc atgcggttaa aatctgattt tttagctcgt tgatttctcg acaaattatt
    17101 ttgtgttctg catgtttttt gagtacatac aaaactgtta gtttttcatt gaatgaattt
    17161 ccaaagtagg ttttcccgat attattttct agttcagcga tcccttgatt aatttcctct
    17221 aattcaaggc tatcgagttg gtttaatagt tttgttttgt acatctactt atgccctcca
    17281 gttcttaatt gatagtcgta ctcgtcataa gcagtgaaga gatataacgc taatcctgca
    17341 aagatagcta tttttgcaat taatggtaat aagccgaaaa ttccgccgat taaaatgctt
    17401 cctataatta agtttcgttt cataatggtt ccctccgatt ttttaatatg gtataatcgg
    17461 aacaaagaat atcgggtttt atcccaatta agttgccttt atcctgtccg ccaagactgt
    17521 tatggataag gctttttctt tgttctcgat catgttaatg ctcctaagtt gtctgcaatt
    17581 cagcagaacg attataaatt ttctcactca ctgaaaactg caacccatat ttttctttaa
    17641 cagtcattaa ttctactgtc tcatctaaaa taggttcacg atcaaccaac atttcaggag
    17701 tcatatcttt cttttttacc attttggaat gaccataaat agttgataca gctttattgg
    17761 ctatcacatt cgctttaatg tagtctactg gttttggttc agtaatagcg tggcttaatt
    17821 tagtcattgc ttctttttgg tgttctttgt ctagcatgcg gaatacttga aagccttcaa
    17881 gtcctgtgga ctggcgtagt tctttgatga tgtcgaaaat ccagtcttga aatgcctcgg
    17941 cttctttttt acggcttttg aatgccatgc ggtaaatatt ttttttcgtt gatgatgttt
    18001 acatcttgtg ttctgcccaa tgaatcgatg accttactag tagtaacccc atctttaggc
    18061 aaagagtgaa tggctctagt aacctgttta agacctaacg cttccgcaac atctttcgcc
    18121 actgcccacc attcatcatt aatatcgaca aatcttataa tgtgtccgtt ccaaatttca
    18181 gttttcattc aacgtcctcc agttcaagcc ctagcaagct cgctaacaac tcaacttgct
    18241 gatacaaaat ttcttgatac tcttcaatag aaagtcgttc tttaaactct gcttgtacta
    18301 aatcgttcaa gccacgcaat actccgctta ccgttggttc ttgtggtagc aatttttgat
    18361 agtccatttg ttcgtacaaa cattcaaacg tttcttctag cgctttgcct tccaagtttg
    18421 ctaattctgc cgctgtgtct tctgtaagaa ttgttgataa tactttttcg attgctttat
    18481 tttgtgtcga ttccattatt atttcctcct attaatccct ttgcccccac ggcttgcgtt
    18541 tatttttggt attcttccat aaagcgatca cattcatctt gtgaaattct tacgactcca
    18601 tcaactataa tcaactttaa accgttagga acatatttct gcatcatagt attgtatgaa
    18661 acgttcatat acttagcagc ttgtgccttg ttcatatatt ttggaaactg gtactttttt
    18721 tctaataatg aaagtaacgt tccttgagtt tgctctactt gttgaacaac tcgatcgtca
    18781 ataattttaa aagctggatc atcaagcgtg agtttgtcaa tttttaattc catatttatc
    18841 ccccctcgtt agcatacatt tctatacaaa gattttaaaa aaatgcaaga ggctcttgaa
    18901 tacatttcta tacctcttat aaactcaata taccatcata ccttttcatt tgcaagaatt
    18961 ttaacatact tttttgaact tttatgtttc acacatattt aaatcgtgat atagtagaga
    19021 aaatatatga atggtggtga aaatgtggct agaaaaaaat accccgaagc agtggagaca
    19081 ggacaaagaa ttagactggc tagaattgaa aagggactct caatggaaca acttggaggt
    19141 aaattatccc caccggccag taaaggtgcc gttagtaatt gggaaaatgg ctataatctt
    19201 cccaataaca ccaggcttaa acaactttca aaggtattag atgtatccac tacctatcta
    19261 ttaaacggat cttatacatt aagagatatg catatgatgc ccgaaattaa gcgaaatgtg
    19321 cttgaaaagc aattgaaaaa aactattgaa aaaaataact ctataattga ttctaatctt
    19381 tctaatgcta tcaataacat cgaagaagat aacctgactg tctcacagaa gatttattta
    19441 acaaacgcaa taaaatatct taaaacagga tctcgaattg aatttcctga cgggagtacc
    19501 ccaattgctt atctcgcagc tcttctaaat acgcttttac gatatgactc attaattaat
    19561 gattcagaaa aaaatacaga tgagaaaaaa gagtatcaag aattttatga aaatgatctt
    19621 ctaaaagagt tcacagctct tgttactgag ctaaataaac actattcaaa ataataataa
    19681 actgcctcct ccaaccgccc ccacggcttg ctacactatg gagggaaaac tattatggca
    19741 acattcaagc aatatgaaac taaaaaaggt aaattttggc tctatgaagc ctatcttgga
    19801 atgaacaaaa tgactggcaa acctgataag gttcgtagac gaggttacaa aacaaaaaaa
    19861 gaagcccagc ttgccttgtc acgcttgcaa gtggactacg ataaaaacgg tattaaaaaa
    19921 gcgaacaacg aaacgtttaa agaagtttat gacttgtggc tagaaacata taaaaccact
    19981 gtccgagaag ccaccttcat gaaaactgaa atcaagttta gaaagtggat tcttccaaaa
    20041 tatggaaatc ttcgtgtgaa tgaagtcacg gtaaaaaaag cccaacaaat tgtaaatagc
    20101 tgggctgaaa ctactgacca atacaaggta cttcattcaa cttctaagcg tatttttagc
    20161 tacgctatca atcttgggat tattgagact aatccactag acaacgtatt aatgcctaaa
    20221 agaaccacca ctaagcataa agaaaaggtc aaagtttaca gtaaagaaca actgatcacc
    20281 ttgtttgatt acctaaacag caaacctgaa acttatcgaa acgactttga taaaacgtta
    20341 ctacgctttc tcttttatag cggggttagg atcagtgaag cccttgcatt gaattggtca
    20401 gacattgatt ttcaagaaaa tacggtcacc atcaataaaa cactaagcca atccaaaaat
    20461 ggctataaaa tcagtgatcc taaaactgat tgtagtgctg gggttcttcc actagatgaa
    20521 agaacaattt ctatattaaa aaaatggcag attaatcagc ggaaatatat gctgacactt
    20581 ggtgtaactg atccgactat gattttttgt ggtatctata aagaactaat cacacaccac
    20641 gctatttatg caagattgat tactatttgt gaaaaagcta tggttccatt tctcggtgtg
    20701 catgtagcaa gacacactca tgcttcaatg ctcttggacg ctggagctac tatggtagaa
    20761 gttcaaacta gattacggca tacaaaaatt agtcagacga tcgattgtta cggtcacttg
    20821 gcaaaagaaa cgaaagaaaa gactgttgaa aaattagtac aacacttaaa tattgactag
    20881 atggcataca atatggcata cagtaaaaaa taaaaaaagg agaaacccta tcatatcaag
    20941 gtttctccta gctttatagt ctattcttct tcttccgcca tgaatgtatt gaatacttct
    21001 tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct
    21061 tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagca
    21121 ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagtaggatt
    21181 tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcatgg
    21241 tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta ttaaaataat
    21301 tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa tttatcgatg
    21361 actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga
    21421 acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc tttgatcttt
    21481 tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat atttttaggt
    21541 aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa ccgatcaaag
    21601 ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc tgtccagcca
    21661 aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc cattactctc
    21721 ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg atctcatcgc
    21781 gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag gccgggtctc
    21841 cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc
    21901 gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac
    21961 gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc
    22021 ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa
    22081 cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct gtagctttat
    22141 caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt ttttacacaa
    22201 ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg tttttgttgc
    22261 ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa agacattgaa
    22321 ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg ctaccagttc
    22381 attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt ctttgatata
    22441 tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac gtgtccctgt
    22501 catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta aatggctgcg
    22561 attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg ggtcaatata
    22621 caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat tggcttgtcc
    22681 tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg aaaggctaag
    22741 atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat gttttccatc
    22801 atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa tagttgtaat
    22861 ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc ctgcaatcac
    22921 agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta
    22981 ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag aaagaaatag
    23041 aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa gagacggatt
    23101 cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt caaaaatttt
    23161 cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg cgcaaaagct
    23221 tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt ttgcttggct
    23281 gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg gtgacgggaa
    23341 tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac acccgcaata
    23401 caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt tacgcttctg
    23461 gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg ctatgacggg
    23521 aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca tgatggaaag
    23581 caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag cttctggaaa
    23641 tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg atcgtacctc
    23701 gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag tagtaaaagt
    23761 aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac tttactgcga
    23821 actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa tcgttagcgc
    23881 ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg atttacctgt
    23941 gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga aattttttag
    24001 aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg ctatctacag
    24061 cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg tgatgccttt
    24121 agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac attatgtctc
    24181 aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt ttgcgattgc
    24241 ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga tatttattca
    24301 gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg tacctaggcg
    24361 ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact ggatctgtgt
    24421 tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag tagcattcat
    24481 cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata ttcccgggat
    24541 ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg ttactgaatc
    24601 ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag caatcgcttt
    24661 aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag taaagcgtga
    24721 cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta acaaagaaaa
    24781 ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa cattgcgaca
    24841 agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt gtaaaaatag
    24901 cttctcacat ttttacataa aattgactta atactgagaa actgactttt gaacaccctc
    24961 tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct ttattcaaat
    25021 tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat tagaactaaa
    25081 ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat tccgtataag
    25141 acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa acccaaactt
    25201 cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag caaccgataa
    25261 tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc aaatagcaag
    25321 ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc gatcccacca
    25381 gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata ttggttcata
    25441 gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat aagattgctg
    25501 ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag gaaaacaaca
    25561 ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc aaaagcaaga
    25621 ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa attaggttct
    25681 tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt ctttttatgg
    25741 gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag ctttggtact
    25801 atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat tccccaaccc
    25861 aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa taatatcgaa
    25921 atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt catccacatg
    25981 attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac tgtttttcta
    26041 tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc cagacaaaaa
    26101 agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag caaaatccct
    26161 agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac aaaaggaatg
    26221 atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc aaaaggcaga
    26281 ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac tgatatactt
    26341 tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc tggcaattta
    26401 acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag ccctgaaaaa
    26461 agattttgag acaaaagata gatagttaat cgctttccct tttttccttt ctctcttgct
    26521 ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc taataattgt
    26581 cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca aataaatctt
    26641 ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct gataaatcaa
    26701 ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat ccaatacatg
    26761 tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt ctttcaaatc
    26821 aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa tatctggatc
    26881 tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa aacttgcgat
    26941 tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta aacgcttttc
    27001 ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt ctccagccgt
    27061 caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt tgatactcaa
    27121 tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg gttcaataat
    27181 actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta aaaatccaaa
    27241 aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt tcccgtaact
    27301 tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta ttacttttta
    27361 ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa aaaaggatag
    27421 aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct tctaacaggt
    27481 aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct tgcataaaat
    27541 tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt gcaaatgtta
    27601 ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata taagctttta
    27661 aatctttaca taaatactca tattcgagtg attttttata ttttttaatt gtgccgttta
    27721 cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct ggcatgctca
    27781 ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa cggacagttg
    27841 tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc aataagtaac
    27901 tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa ccactaggcg
    27961 taatctttcg attaataaca gttattgctt tattcggatg agacgttaga taaacaattt
    28021 tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta taataggcgt
    28081 tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt ttaatgaatt
    28141 caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga cttaataaaa
    28201 ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta tcatccaaaa
    28261 taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc aaaatcattt
    28321 catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac aatcttttcc
    28381 aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat aaatatcaaa
    28441 caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg tcagctgtct
    28501 gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg atttttcgga
    28561 gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt gaatggttga
    28621 taatagtctg attggatggt tccttctatt ttataccatt catcattttt aaccgttact
    28681 ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca atggataatg
    28741 ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta aacaaatcct
    28801 tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag ctccataact
    28861 tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt ttctttcaac
    28921 tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc cggctttaag
    28981 tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa attaaatcct
    29041 ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc cacgattact
    29101 ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa cccatggtca
    29161 tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa atacagctga
    29221 actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg ataatggaca
    29281 ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc gaaataccca
    29341 gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga atccaataac
    29401 gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat atcgtttcat
    29461 catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag ctacaggtgc
    29521 tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg agcaaagaga
    29581 catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg agccgagcgt
    29641 tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc cgccaatgat
    29701 caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat gcccagtttg
    29761 atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat ggacatgatg
    29821 atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt ctttttgaaa
    29881 ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca cccgccagct
    29941 tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga ttggattgat
    30001 gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt ttttaacaaa
    30061 ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc ctaagatcga
    30121 accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac gttctggggt
    30181 caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag gtaatgcttc
    30241 aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa taaaagaaaa
    30301 catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg cttttattca
    30361 aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca aagcacttca
    30421 attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact tctatcataa
    30481 aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac atactgcaac
    30541 agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt cacgctgttc
    30601 gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat ttggatgcat
    30661 ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt gtcctgattt
    30721 ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa aaaatccgtt
    30781 atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa atcttccgaa
    30841 caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt ttgccttcat
    30901 ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat ttgcgtcaga
    30961 atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt ggatcaatac
    31021 atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga ttcctgcttc
    31081 cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa ttgctacttc
    31141 gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg ggataccgtc
    31201 tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac gtatgctatt
    31261 actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac cgatcagcgc
    31321 aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa gatcttctac
    31381 atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc gacggatcgt
    31441 tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt tctttccagc
    31501 attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg tcgcctttgg
    31561 aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc tttgatttct
    31621 ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt gtgaaatttt
    31681 attctttatt catttattta tcatttctgc aaatctcgca gtataatgga atttatggta
    31741 gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag aaaagaggat
    31801 tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga agtcttattt
    31861 gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg cagaaatggc
    31921 gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga tgaaggaaag
    31981 atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg acttgattcc
    32041 aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca aatggaagag
    32101 cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc tgcttatagt
    32161 tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa cggctatggt
    32221 tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc attttatgat
    32281 cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc tcgcatgctt
    32341 ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga tatcgagaca
    32401 ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat tgtttcccat
    32461 gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag aagaaaaatg
    32521 cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca gttagcaagt
    32581 gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga cttcgttgct
    32641 cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa gacattagaa
    32701 aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt tctattcagt
    32761 atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat cggatatgat
    32821 aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc gattgctcta
    32881 gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca ccagatccct
    32941 tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta cgatcaaggt
    33001 caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga gcatcctacc
    33061 actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg cgaagatgta
    33121 gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt agccaagtta
    33181 gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt agatattgac
    33241 aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct ttttgtttcc
    33301 catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc tgaaaatggc
    33361 agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga agaagaagaa
    33421 atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac gaacaaatct
    33481 gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg aaaaattact
    33541 caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga acaagaaatg
    33601 gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca agatttagaa
    33661 gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag cttggaatta
    33721 gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg ttttggtttt
    33781 tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa taagaagacc
    33841 aattactagt cattctcact aaagcttcta aataatagta atcaccccaa atacaaggtg
    33901 tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag ccatcttctt
    33961 cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca gcagactggt
    34021 ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat aatccacaaa
    34081 ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca aagatcaaat
    34141 cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct aatattttgc
    34201 ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa ccgtaaatcc
    34261 cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc gtacttccat
    34321 atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa ccatcttctc
    34381 ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta tatttttgat
    34441 ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa tccatgatca
    34501 ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact cctgccttct
    34561 cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc atttttcgat
    34621 ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca agatcgtgat
    34681 gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct ttttcagctg
    34741 tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt cctgtccaaa
    34801 atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata ggacactcgc
    34861 ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg tgtctgcgac
    34921 aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat tcatttatcc
    34981 atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc ccaccagcct
    35041 ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc cggttgtccc
    35101 atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc gatcttgatg
    35161 agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct ctaatccagg
    35221 aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa tagctgtgga
    35281 ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag cttcactcac
    35341 cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag aacattcaat
    35401 agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt gtacgcgaat
    35461 ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag taggttccca
    35521 tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc gaacgatgaa
    35581 ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg tgtccgctcg
    35641 ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg ctcgcggtat
    35701 cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg tcaatgtatg
    35761 gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat catgttgtat
    35821 gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag gcatttcttt
    35881 agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct tattcagcca
    35941 taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt aggtataacc
    36001 aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc ttatattccg
    36061 attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc acaacatagc
    36121 tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag gcacatttgc
    36181 accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc ggttcttata
    36241 tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat aataatgaaa
    36301 tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc agccattttg
    36361 aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt taactggata
    36421 accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat taccatctgc
    36481 acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt ctcctgtatc
    36541 taattttttc cataataatt gttcatgatg atataacgct aataacattg cgggcatttc
    36601 taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg aaggatctgt
    36661 tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt ggtcaattgc
    36721 gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt tatttccata
    36781 tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa acggctggag
    36841 tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta atagtggtac
    36901 tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc cgactgttct
    36961 aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac attataagtc
    37021 agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc catattccaa
    37081 tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg atacattttt
    37141 gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc gcatactcca
    37201 gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat ataaccagtt
    37261 ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag atagtacgga
    37321 ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt tcctctgctc
    37381 caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt ccctgattct
    37441 gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata cttagctgtt
    37501 gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc cctcgaagcc
    37561 caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg tttatggtca
    37621 ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac cgtagctgca
    37681 tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc aatagccgat
    37741 ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag ggcagattct
    37801 aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt cttgtgaaag
    37861 agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc tctgtccttt
    37921 attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc cattctgtct
    37981 aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca ttccttcgtc
    38041 tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag ttgctcctag
    38101 aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc caatgtcatt
    38161 ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca ctgaagttgc
    38221 taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa ccgtcatatt
    38281 tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta atggaaatag
    38341 atattgattc caacttccca taaagataag taaaccacta gtaataatcg taggtgataa
    38401 aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga ttctggcagc
    38461 ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca ctgaagttgt
    38521 actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta aacgactcat
    38581 aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata gcataactgt
    38641 attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac ccgccattgt
    38701 agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat tccaaataat
    38761 caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca tatccccaaa
    38821 cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag ctacaatcat
    38881 atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt acgtaaaaat
    38941 tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat tgaatagccg
    39001 ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt ccaaaatcaa
    39061 ccatttcaaa acttgtttta tataaattga ccctacgacc aaagctgaat tttctggtcc
    39121 attggaagtc ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc
    39181 taaaactgtg gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg
    39241 ccgaaatttc gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg
    39301 aagaccagct aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat
    39361 cattgatatt cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc
    39421 attcaaaaat ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac
    39481 agccaccgtt gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt
    39541 taactgtgct gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat
    39601 tgatcctgcc atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa
    39661 gatagctaga taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact
    39721 aaaagcaaac gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg
    39781 tgtgaccaat ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt
    39841 ctttattgtc ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt
    39901 aatcaccagt taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg
    39961 aattttcaaa atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag
    40021 catctgaata agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata
    40081 gttcatagta tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat
    40141 aggccaatga gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat
    40201 ctgtcagcat aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga
    40261 cataaaaact tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa
    40321 tcagtggagc catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag
    40381 cataccagcc gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg
    40441 caccgtagaa atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt
    40501 tcgcttcttt agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct
    40561 gggctagcat gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac
    40621 taccagtttt ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt
    40681 cttcaaaatc tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg
    40741 taaaatcact agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca
    40801 atttttgatc ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat
    40861 aaaaagcatc ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa
    40921 tatccggcat atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc
    40981 ccgaagctac attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt
    41041 ttgcagcatt ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag
    41101 tggctttttt tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac
    41161 atgcacttaa gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt
    41221 ttttcatgtc tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta
    41281 tattaacgga attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg
    41341 cttttcattt ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga
    41401 gaaaggaaag tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca
    41461 ctaacctatt ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta
    41521 ataaatgaaa gcgctaattt atatgctgaa tttgcacata aatctataga actagataat
    41581 ccttcccttt tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa
    41641 acacaaactt ttatcattat ccagttttat tttgatgagc aattattagc acatattacg
    41701 ggacaatctt tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa
    41761 cagttcaaaa ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc
    41821 aattcttttt tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact
    41881 tattgtctgc aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct
    41941 ttattgacaa taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca
    42001 gatgaattag ttgatccaat catacgatat ttagaagaac atctttcaga aaattttttg
    42061 cttgaacaat tagcccaaat tttttttatc agtccagcca gcttaaaaaa acattttaga
    42121 aaagtgaatg gaaaaagtgt tattagctat tttaaagaac taaaaatgga aaaagcaaaa
    42181 cgtctcgtgc tagaaaatga attaacctat acagaaattg cagatatatt aggatttcat
    42241 tcgatccatc atttttcttc agcttttaaa aaatatacag ggttatcccc ttcaaagtat
    42301 tatctatcct taaaatagaa aaaaattttt aggttttatc cacaaattat ttctctatat
    42361 tttctgtcta ttcaaaataa acaataattt tccggcacaa ttatttgctg ttttttacca
    42421 taacatagta cgatcaacgt agaaaaggag atgtttttat gaaagttgtt gtcattggcg
    42481 gtgtagctgg cggtccttca tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa
    42541 tcattatcta tgaacgcgga gaaaatattt cttatgcaag ttgtgctttg ccttattatt
    42601 taggaggtgt gatcactgac cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa
    42661 caaaaaacaa catcgatgtc tttactaaac acgaagtaac agcaatcgat ccttctacta
    42721 agcgattaac agttaaagac ctatccacaa atgaagaaat aaaaacagat tacgataagt
    42781 tgatcatctc ttctggtgct agaccagatt atccggatat tcccggagtt tttgaagcag
    42841 aaaacggctt tgtcctccgt agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag
    42901 aaaaaaatcc acaacatgtc gtcattcttg gtgcaggtgt tatgggtctg gaattagctg
    42961 agaatttcaa gcatcgcggc ttagacgtga ctttaatcga tcaattgcca caagtcgctt
    43021 tcccttatga tccagaaatt gctaatttag tttatgacaa attgctgaaa gaaggattag
    43081 ccgttcattt agaaacaaga gttactgaga tccgtgataa aggtcgagaa atagtattat
    43141 cagatggctc cgtcctttct gctgatatgc taatttttgc tgttggtgtt tctccgaaga
    43201 atgaagtggt gaaagcaacc ggcatacaat tatcagatac aggtcagatc attgttgatg
    43261 accagttaca aaccaatctg ccggacatct atgcgattgg cgatattatc gaaacgacta
    43321 gtgtagtgac tggtcagccg atccaaagta tgctttccag tgcggccaat cgtcaaggac
    43381 atatgctggc agatatttta aatggtacgc ctatgcgcta tcgcggatat attggtgcag
    43441 gtgtcgcaaa aatctttgat catacagcaa gttatgcggg aatgacagaa catgaattaa
    43501 aagcatcagg cataacaaat tataaaactg tttttatcac tccctttgac catgcctatt
    43561 tctatccaga agctacaaga ttaaatctaa agctgatttt tgatgcagat agcggtcgta
    43621 ttttaggtgg acaagcattt ggagaaaaag gtgtcgataa acggatggga gaactttctg
    43681 tagcaatcac cggaaacttg acagtctttg atttgcctga tttggagttg ccttactctc
    43741 caccgtattc tactacccgt gacccgttga atatagctgg ttatgtcgca atcaatcaaa
    43801 tgacgaatat cgtagaaacg atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag
    43861 cgttcttttt agatatacgt gaacctaata aagcaccttc tggcagtatc tcggcaacaa
    43921 aaaatattcc aatgaatgag ttacgagatc gaatcaatga aatcccaaaa gataaaaaag
    43981 tttacattac tttcagaaga ggattgaata cttatacttc tgcccgaatc ttggcaggtt
    44041 tgggtatcaa agcgattttg attgaagaat aaaaaaacga ccgcggtggg tagccgcggt
    44101 ctaggagtta tttttacttg aggagtaaaa atgaaaaagt atatagggtt gttggttggt
    44161 atgtatatag aatagaagat aaatgtgaag aaattgtgca ttatttcttc acatttatta
    44221 ggttatagtt taagaaagtt taaacattgc gcaatttaat tattttagct gtttcttttc
    44281 ataaaaaaac atgttgtgac tctgagacaa atcatagtcc atagtgatgt ctctagagta
    44341 actacgccta atattacgag gcatcatatt aatctatcag cactagtacc taaactagca
    44401 tccctttcga atattttttc ttatataata aagtttgctt tcactcctct aatctcacca
    44461 aatcgactgg cttgcgttgt gtgatactag gaactgattg gtttagaacg acttttctat
    44521 tgtaatcata tttttcgcca tcctcgttgt cttctaatcc ttcaatgaat gatccctaat
    44581 gtgtgtatta aataatttaa tttttttgtt cctatttatt ta
//
LOCUS       DACIWG010000023        41205 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.023, whole genome shotgun sequence.
ACCESSION   DACIWG010000023 DACIWG010000000
VERSION     DACIWG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.023"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(26..328)
                     /locus_tag="IYF54_001262"
     CDS             complement(26..328)
                     /locus_tag="IYF54_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831601.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IYF54_001263"
     CDS             complement(353..2446)
                     /locus_tag="IYF54_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9831602.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IYF54_001264"
     CDS             complement(2935..3699)
                     /locus_tag="IYF54_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ9831603.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IYF54_001265"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IYF54_001265"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ9831604.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IYF54_001266"
     CDS             complement(4704..5780)
                     /locus_tag="IYF54_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ9831605.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IYF54_001267"
     CDS             complement(5804..6397)
                     /locus_tag="IYF54_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ9831606.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IYF54_001268"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IYF54_001268"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ9831607.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IYF54_001269"
     CDS             complement(8067..8366)
                     /locus_tag="IYF54_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ9831608.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IYF54_001270"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IYF54_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ9831609.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IYF54_001271"
     CDS             complement(10121..11032)
                     /locus_tag="IYF54_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ9831610.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IYF54_001272"
     CDS             11152..12009
                     /locus_tag="IYF54_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9831611.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IYF54_001273"
     CDS             complement(12196..12558)
                     /locus_tag="IYF54_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831612.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IYF54_001274"
     CDS             12904..14343
                     /locus_tag="IYF54_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9831613.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IYF54_001275"
     CDS             complement(14350..16902)
                     /locus_tag="IYF54_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ9831614.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IYF54_001276"
     CDS             complement(17016..18347)
                     /locus_tag="IYF54_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9831615.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IYF54_001277"
     CDS             complement(18313..19791)
                     /locus_tag="IYF54_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9831616.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IYF54_001278"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IYF54_001278"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ9831617.1"
                     /translation="MKDMDIKVVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IYF54_001279"
     CDS             22401..23828
                     /locus_tag="IYF54_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ9831618.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLHEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IYF54_001280"
     CDS             complement(23920..24285)
                     /locus_tag="IYF54_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ9831619.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IYF54_001281"
     CDS             complement(24282..24647)
                     /locus_tag="IYF54_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ9831620.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IYF54_001282"
     CDS             complement(24839..25087)
                     /locus_tag="IYF54_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831621.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IYF54_001283"
     CDS             25086..25214
                     /locus_tag="IYF54_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ9831622.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IYF54_001284"
     CDS             25271..26965
                     /locus_tag="IYF54_001284"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ9831623.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IYF54_001285"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IYF54_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IYF54_001286"
     CDS             27170..27361
                     /locus_tag="IYF54_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831624.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IYF54_001287"
     CDS             27739..28872
                     /locus_tag="IYF54_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ9831625.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IYF54_001288"
     CDS             28903..29223
                     /locus_tag="IYF54_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831626.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IYF54_001289"
     CDS             complement(29252..29716)
                     /locus_tag="IYF54_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831627.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31100)
                     /locus_tag="IYF54_001290"
     CDS             complement(29847..31100)
                     /locus_tag="IYF54_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9831628.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31129..31446)
                     /locus_tag="IYF54_001291"
     CDS             complement(31129..31446)
                     /locus_tag="IYF54_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9831629.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31472..31792)
                     /locus_tag="IYF54_001292"
     CDS             complement(31472..31792)
                     /locus_tag="IYF54_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831630.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32256..33671)
                     /locus_tag="IYF54_001293"
     CDS             complement(32256..33671)
                     /locus_tag="IYF54_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9831631.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33688..35004)
                     /locus_tag="IYF54_001294"
     CDS             complement(33688..35004)
                     /locus_tag="IYF54_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9831632.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35154..35852)
                     /locus_tag="IYF54_001295"
     CDS             complement(35154..35852)
                     /locus_tag="IYF54_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ9831633.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36141..37130)
                     /locus_tag="IYF54_001296"
     CDS             complement(36141..37130)
                     /locus_tag="IYF54_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ9831634.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IYF54_001297"
     CDS             complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IYF54_001297"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9831635.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38218..39852)
                     /locus_tag="IYF54_001298"
     CDS             complement(38218..39852)
                     /locus_tag="IYF54_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831636.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39839..41173)
                     /locus_tag="IYF54_001299"
     CDS             complement(39839..41173)
                     /locus_tag="IYF54_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831637.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12502 a         8475 c         6957 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacgac cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt acatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
    30841 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
    30901 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
    30961 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
    31021 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
    31081 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
    31141 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
    31201 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
    31261 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
    31321 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
    31381 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
    31441 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
    31501 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
    31561 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
    31621 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
    31681 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
    31741 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
    31801 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
    31861 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
    31921 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
    31981 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
    32041 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
    32101 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
    32161 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
    32221 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
    32281 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
    32341 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    32401 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    32461 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    32521 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    32581 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    32641 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    32701 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    32761 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    32821 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    32881 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    32941 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    33001 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    33061 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    33121 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    33181 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    33241 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    33301 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    33361 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    33421 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    33481 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    33541 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    33601 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    33661 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    33721 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    33781 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    33841 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    33901 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    33961 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    34021 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    34081 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    34141 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    34201 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    34261 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    34321 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    34381 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    34441 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    34501 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    34561 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    34621 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    34681 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    34741 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    34801 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    34861 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    34921 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    34981 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    35041 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    35101 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    35161 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    35221 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    35281 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    35341 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    35401 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    35461 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    35521 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    35581 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    35641 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    35701 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    35761 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    35821 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    35881 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    35941 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    36001 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    36061 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    36121 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    36181 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    36241 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    36301 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    36361 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    36421 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    36481 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    36541 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    36601 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    36661 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    36721 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    36781 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    36841 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    36901 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    36961 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    37021 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    37081 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    37141 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    37201 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    37261 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    37321 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    37381 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    37441 taattctttt tgtcgttcgc cttgataaaa aatcactgga cgatattgct tccctctatc
    37501 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    37561 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    37621 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    37681 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    37741 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    37801 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    37861 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    37921 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    37981 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    38041 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    38101 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    38161 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    38221 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    38281 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    38341 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    38401 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    38461 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    38521 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    38581 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    38641 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    38701 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    38761 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    38821 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    38881 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    38941 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    39001 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    39061 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    39121 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    39181 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    39241 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    39301 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    39361 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    39421 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    39481 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    39541 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    39601 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    39661 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    39721 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    39781 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    39841 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    39901 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    39961 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    40021 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    40081 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    40141 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    40201 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    40261 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    40321 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    40381 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    40441 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    40501 tacctaagat acgccaagga aactggagca agcttatcgg tgttttttga aacaaaaacc
    40561 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    40621 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    40681 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    40741 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    40801 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    40861 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    40921 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    40981 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    41041 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    41101 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    41161 aagcagcatc tatcttttta aaaaactttt ctccataata aaaac
//
LOCUS       DACIWG010000024        40742 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.024, whole genome shotgun sequence.
ACCESSION   DACIWG010000024 DACIWG010000000
VERSION     DACIWG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.024"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            23..646
                     /locus_tag="IYF54_001300"
     CDS             23..646
                     /locus_tag="IYF54_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831638.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IYF54_001301"
     CDS             1096..2145
                     /locus_tag="IYF54_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9831639.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IYF54_001302"
     CDS             2142..3023
                     /locus_tag="IYF54_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831640.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IYF54_001303"
     CDS             3026..3796
                     /locus_tag="IYF54_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9831641.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IYF54_001304"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IYF54_001304"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ9831642.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IYF54_001305"
     CDS             5107..6270
                     /locus_tag="IYF54_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ9831643.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IYF54_001306"
     CDS             6387..6797
                     /locus_tag="IYF54_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9831644.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IYF54_001307"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IYF54_001307"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ9831645.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVILYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IYF54_001308"
     CDS             9337..9654
                     /locus_tag="IYF54_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831646.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IYF54_001309"
     CDS             9814..10050
                     /locus_tag="IYF54_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9831647.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IYF54_001310"
     CDS             10047..10445
                     /locus_tag="IYF54_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ9831648.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IYF54_001311"
     CDS             complement(11112..12056)
                     /locus_tag="IYF54_001311"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ9831649.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IYF54_001312"
     CDS             complement(12352..13296)
                     /locus_tag="IYF54_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831650.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IYF54_001313"
     CDS             13517..14731
                     /locus_tag="IYF54_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9831651.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IYF54_001314"
     CDS             14753..16768
                     /locus_tag="IYF54_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ9831652.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IYF54_001315"
     CDS             complement(16851..18224)
                     /locus_tag="IYF54_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ9831653.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IYF54_001316"
     CDS             complement(18250..18534)
                     /locus_tag="IYF54_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831654.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IYF54_001317"
     CDS             complement(18536..20551)
                     /locus_tag="IYF54_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9831655.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IYF54_001318"
     CDS             complement(20730..21035)
                     /locus_tag="IYF54_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ9831656.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IYF54_001319"
     CDS             complement(21124..22155)
                     /locus_tag="IYF54_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9831657.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFPLAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IYF54_001320"
     CDS             22362..23237
                     /locus_tag="IYF54_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9831658.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IYF54_001321"
     CDS             complement(23389..24231)
                     /locus_tag="IYF54_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9831659.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IYF54_001322"
     CDS             complement(24323..26200)
                     /locus_tag="IYF54_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9831660.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IYF54_001323"
     CDS             complement(26197..27618)
                     /locus_tag="IYF54_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9831661.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IYF54_001324"
     CDS             complement(27621..31010)
                     /locus_tag="IYF54_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ9831662.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32586
                     /locus_tag="IYF54_001325"
     CDS             31267..32586
                     /locus_tag="IYF54_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9831663.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEEYVVQLLTQ
                     IYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSS
                     YIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITED
                     QLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(32659..33306)
                     /locus_tag="IYF54_001326"
     CDS             complement(32659..33306)
                     /locus_tag="IYF54_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831664.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33314..34000)
                     /locus_tag="IYF54_001327"
     CDS             complement(33314..34000)
                     /locus_tag="IYF54_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831665.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34006..35079)
                     /locus_tag="IYF54_001328"
     CDS             complement(34006..35079)
                     /locus_tag="IYF54_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ9831666.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35301..35975
                     /locus_tag="IYF54_001329"
     CDS             35301..35975
                     /locus_tag="IYF54_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831667.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35980..37320
                     /locus_tag="IYF54_001330"
     CDS             35980..37320
                     /locus_tag="IYF54_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9831668.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37469..38455)
                     /locus_tag="IYF54_001331"
     CDS             complement(37469..38455)
                     /locus_tag="IYF54_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ9831669.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTYDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39179..40210
                     /locus_tag="IYF54_001332"
     CDS             39179..40210
                     /locus_tag="IYF54_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ9831670.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40319..>40742
                     /locus_tag="IYF54_001333"
     CDS             40319..>40742
                     /locus_tag="IYF54_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ9831671.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12642 a         7400 c         7647 g        13053 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcattcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactagat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgg
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgtaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat
    32101 tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt
    32161 gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa
    32221 ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg
    32281 atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc
    32341 agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct
    32401 gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat
    32461 cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat
    32521 atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag
    32581 ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat
    32641 acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa
    32701 gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat
    32761 tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa
    32821 atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc
    32881 tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata
    32941 gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa
    33001 atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct
    33061 tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg
    33121 cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca
    33181 aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa
    33241 agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat
    33301 tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc
    33361 gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt
    33421 tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat
    33481 ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag
    33541 caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg
    33601 actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag
    33661 taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc
    33721 tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct
    33781 cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg
    33841 aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc
    33901 cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa
    33961 atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc
    34021 tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac
    34081 aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc
    34141 gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac
    34201 cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg
    34261 tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc
    34321 gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg
    34381 ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt
    34441 cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg
    34501 ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga
    34561 cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa
    34621 aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag
    34681 attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg
    34741 taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt
    34801 gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc
    34861 tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc
    34921 ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc
    34981 tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg
    35041 taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata
    35101 gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca
    35161 cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac
    35221 atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac
    35281 caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat
    35341 ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta
    35401 gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga
    35461 tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc
    35521 cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag
    35581 gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag
    35641 cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca
    35701 gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa
    35761 aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc
    35821 agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt
    35881 atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct
    35941 tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga
    36001 aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt
    36061 ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca
    36121 ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa
    36181 ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca
    36241 ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt
    36301 gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg
    36361 gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct
    36421 gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac
    36481 atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt
    36541 atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct
    36601 agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat
    36661 gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt
    36721 accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag
    36781 acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat
    36841 cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa
    36901 ccatttcaag agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca
    36961 ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat
    37021 gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa
    37081 gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg
    37141 atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg
    37201 cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc
    37261 aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa
    37321 tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa
    37381 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca
    37441 cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta
    37501 catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa
    37561 catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta
    37621 catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg
    37681 ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga
    37741 ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag
    37801 ttgtcacatc atatgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat
    37861 ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc
    37921 caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa
    37981 agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc
    38041 ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg
    38101 gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt
    38161 gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag
    38221 tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg
    38281 tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag
    38341 tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata
    38401 atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa
    38461 ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt attctatagg
    38521 agacgattgt tttctacaaa tatttagatt ggaatttgat taaaacaatt taattgaaat
    38581 ttgaaaacat ttatgttaat gaaagtcata ttctaataaa atgtcatttg tttctatatg
    38641 aatttttatg aaaaatttca tataatttcc actcgttgtg atggtttaat ttatccaatc
    38701 catcaataag caaacaactt atataaaaaa ggattaattc tcaatataat tgctcctacc
    38761 atagaataaa tcttcatgta aagtaatgaa gtaccaacta tttctaatac ttctttaaga
    38821 attatcccaa caatatcgtt agacaaacca ttattgaata aatggagagc caatcgattc
    38881 tgaaaaaatg ttttctaatt tttcggaaat aaaagaaaga gcctcctgcc attagaacga
    38941 accaaatcca tttcattgct catgaagtaa ccacatagct aaaaagagga taaatcaatt
    39001 aaaagatcaa aagaatgact gcactaataa aataactaaa aatgtatctt cttacttcat
    39061 atcttttagc tagtacttag ttaaaaattt gcttcctaag atcaagacaa actgtctatc
    39121 ttcatttgat gaaatgcggt aaaatataga aaatgaacac tattggaggg aatttgtaat
    39181 ggacagcaaa actagaaaac aactgcaaga aaagttaaag caacgtacac acttgctccc
    39241 ttccgtcttt caacagtttt tagaagaaaa tcgttcttcc ctttcattag gcagtcgcta
    39301 tgaatacgca aaagattttc atctgtttac tgattatttg agaaaagtat tttctgaaga
    39361 agtggatgat cagctgatta tccatttaga aaaacaagat tatcagaatt tcttagctaa
    39421 aatctatcga catactcgtt cgttccaaac tacagcgaac caagaaacgg agcaattgtt
    39481 tgaaaatagc tattacggga tttcaaggaa gcaatattca ttaaatcatt ttttacgatt
    39541 tttgtatcaa aaaggactga ttgaagaaga gatttctctt ttgggtacaa gtttaccgga
    39601 aacaaccaaa gcagctccgc gatatatcga tgcagaactt ttcaaagatt ttgagcattt
    39661 gtttatcccg attgaaggct ttcaaacaag cagagaagct agctttgaag aaaagaatag
    39721 accgaggaac cttgcaatta ctgccattct tctgtattgc ggattaaaga tccatgaagt
    39781 agtagaactt aaacgtaaag atgtatcttt gaccaagcag attcttaatc tgaatagaaa
    39841 agaaaacaga ctgaataaag taccaatacc aaaagaagcg atgccatttc tggagaacta
    39901 ttttaagtta acagcacaaa atacggacga aaattctttt gtttttcgtt cctcacatga
    39961 tcagcaaatt tctcctcgga ctattcgcca aattttgagc aaaattacag tatataaaaa
    40021 cgtatcgcca gaactatgca gaaaaacata tcgttattat gcagaactat atttagatga
    40081 tgctgacgcg gtaaattatt tatgtggtaa tcgatacctc tcatcccatt cttttcaaga
    40141 tatctgggaa aagatggttg atttttctta tcacgaatta gcggcattcg ttcgggtttc
    40201 ttccatataa aacaaatgta tgatcttttt ttcacaataa actatgttat tatataaagg
    40261 attatgttac gattatcttg aatttgtata ttacttaaca aaagataatg gagtgtgaat
    40321 gatggagacc ataacgtatt cccctttgaa cttatttacg aattttcaag aagctgcagc
    40381 taacttttcc gagaccccga ttatctttga tgagccactt tcagctttcc cagaattagg
    40441 acttgaaaca acttacaaaa aatgctgcga aagtgtgttg aaacgggctt atcaattggc
    40501 tcatcttgga gtgaaatccg gagacaaagt tattatttat aagagttccg cttttgacac
    40561 ataccttcta gctgtttctg tgtcttacct tggtgcagtg cctgtcatga cttcttatca
    40621 tctaccaaca acaacaatgg aagtatttat cgatcgttta gaagaccctt tcattttatt
    40681 tgatgatgag acaaaaaagc gagtgagaga aatttcaaat ggaacaaaat caaaacagat
    40741 tc
//
LOCUS       DACIWG010000025        40647 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.025, whole genome shotgun sequence.
ACCESSION   DACIWG010000025 DACIWG010000000
VERSION     DACIWG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.025"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(45..1928)
                     /locus_tag="IYF54_001334"
     CDS             complement(45..1928)
                     /locus_tag="IYF54_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ9831672.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2194..3069)
                     /locus_tag="IYF54_001335"
     CDS             complement(2194..3069)
                     /locus_tag="IYF54_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9831673.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYF54_001336"
     CDS             complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYF54_001336"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ9831674.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4962..6254)
                     /locus_tag="IYF54_001337"
     CDS             complement(4962..6254)
                     /locus_tag="IYF54_001337"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ9831675.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSIPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6648..7595
                     /locus_tag="IYF54_001338"
     CDS             6648..7595
                     /locus_tag="IYF54_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ9831676.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7684..8943)
                     /locus_tag="IYF54_001339"
     CDS             complement(7684..8943)
                     /locus_tag="IYF54_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ9831677.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9114..9560)
                     /locus_tag="IYF54_001340"
     CDS             complement(9114..9560)
                     /locus_tag="IYF54_001340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ9831678.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9571..11784)
                     /locus_tag="IYF54_001341"
     CDS             complement(9571..11784)
                     /locus_tag="IYF54_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ9831679.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12017..12781)
                     /locus_tag="IYF54_001342"
     CDS             complement(12017..12781)
                     /locus_tag="IYF54_001342"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ9831680.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYF54_001343"
     CDS             complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYF54_001343"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ9831681.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13742..14230)
                     /locus_tag="IYF54_001344"
     CDS             complement(13742..14230)
                     /locus_tag="IYF54_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ9831682.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14196..14867)
                     /locus_tag="IYF54_001345"
     CDS             complement(14196..14867)
                     /locus_tag="IYF54_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ9831683.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15005..16291
                     /locus_tag="IYF54_001346"
     CDS             15005..16291
                     /locus_tag="IYF54_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ9831684.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16363..16557
                     /gene="ssrS"
                     /locus_tag="IYF54_001347"
     ncRNA           16363..16557
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IYF54_001347"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16635..16919
                     /locus_tag="IYF54_001348"
     CDS             16635..16919
                     /locus_tag="IYF54_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831685.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            16992..17471
                     /locus_tag="IYF54_001349"
     CDS             16992..17471
                     /locus_tag="IYF54_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9831686.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17694..17837
                     /locus_tag="IYF54_001350"
     CDS             17694..17837
                     /locus_tag="IYF54_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ9831687.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18415..19998
                     /locus_tag="IYF54_001351"
     CDS             18415..19998
                     /locus_tag="IYF54_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9831688.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20435..21619)
                     /locus_tag="IYF54_001352"
     CDS             complement(20435..21619)
                     /locus_tag="IYF54_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ9831689.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21644..22651)
                     /locus_tag="IYF54_001353"
     CDS             complement(21644..22651)
                     /locus_tag="IYF54_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9831690.1"
                     /translation="MLPEKMEQGFNQKLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22823..23182)
                     /locus_tag="IYF54_001354"
     CDS             complement(22823..23182)
                     /locus_tag="IYF54_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831691.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23154..23525)
                     /locus_tag="IYF54_001355"
     CDS             complement(23154..23525)
                     /locus_tag="IYF54_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ9831692.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23542..23844)
                     /locus_tag="IYF54_001356"
     CDS             complement(23542..23844)
                     /locus_tag="IYF54_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831693.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23861..24331)
                     /locus_tag="IYF54_001357"
     CDS             complement(23861..24331)
                     /locus_tag="IYF54_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ9831694.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24306..24584)
                     /locus_tag="IYF54_001358"
     CDS             complement(24306..24584)
                     /locus_tag="IYF54_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ9831695.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24581..25630)
                     /locus_tag="IYF54_001359"
     CDS             complement(24581..25630)
                     /locus_tag="IYF54_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ9831696.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYF54_001360"
     CDS             complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYF54_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ9831697.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26648..28402
                     /gene="ade"
                     /locus_tag="IYF54_001361"
     CDS             26648..28402
                     /gene="ade"
                     /locus_tag="IYF54_001361"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ9831698.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYF54_001362"
     CDS             complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYF54_001362"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ9831699.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29232..30011)
                     /locus_tag="IYF54_001363"
     CDS             complement(29232..30011)
                     /locus_tag="IYF54_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ9831700.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30218..31429)
                     /locus_tag="IYF54_001364"
     CDS             complement(30218..31429)
                     /locus_tag="IYF54_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831701.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31422..32159)
                     /locus_tag="IYF54_001365"
     CDS             complement(31422..32159)
                     /locus_tag="IYF54_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831702.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32297..32731
                     /locus_tag="IYF54_001366"
     CDS             32297..32731
                     /locus_tag="IYF54_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ9831703.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32724..33068
                     /locus_tag="IYF54_001367"
     CDS             32724..33068
                     /locus_tag="IYF54_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ9831704.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33248..34258
                     /locus_tag="IYF54_001368"
     CDS             33248..34258
                     /locus_tag="IYF54_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9831705.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDYLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34327..35271)
                     /locus_tag="IYF54_001369"
     CDS             complement(34327..35271)
                     /locus_tag="IYF54_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ9831706.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35255..37975)
                     /locus_tag="IYF54_001370"
     CDS             complement(35255..37975)
                     /locus_tag="IYF54_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9831707.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37972..39192)
                     /locus_tag="IYF54_001371"
     CDS             complement(37972..39192)
                     /locus_tag="IYF54_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ9831708.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39473..40318)
                     /locus_tag="IYF54_001372"
     CDS             complement(39473..40318)
                     /locus_tag="IYF54_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9831709.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12033 a         8442 c         7090 g        13082 t
ORIGIN      
        1 ttcaggcaaa aacaaatgtt tttgcctgaa gttttttgta gctatcattc tgtcacgttt
       61 gtctcactgc tgttcgtact tgaacttcct cctagcaagg cttcgctgac taagtgaatg
      121 taatgtgtca tcccttcagg atttggatgg acttgatcat cataaaacca gtcactatgg
      181 tcattactgt agctgtacca atcaatcagc gtcatattgt cgtatttttt cgtcatctgt
      241 tcaagcatcg cattcacgtc attttgccag cgcttcgtcg gtactcggac attgacccag
      301 tatacttttc gatcaccgat tgcatccatc acactatcaa actgagcttc cgagaatgag
      361 ccattcgttc ctagtccgat cagaacagtg tctttcaaca aatcttgttc ctttaatttt
      421 tcaatgtacg gtacactggc atataattgt cgtccgacat ctccatctac cactactttt
      481 ggataaagtt cttgtagatc cgttgcagca tccaacataa ctgaatcacc aaatgccgtt
      541 agttctaatt tttgtgcctt attcccttgt gccttggaca actcgtactt atcaaggatc
      601 gcttggtcaa ccgaactagt ggtgttttct gaattgtttc tggtttcttc tgctaatttt
      661 ttactttcag cgatttttgc ctgcagttgt ttttgatcgg ctgttacagc gtttttcggt
      721 gccgtaacga aaccaacgag tgcaattgct acaagtaatg tgcctggaac aacccatggt
      781 ttgctcttac tgatgatcgg tttgctgaac catcctttca tcacttggaa tgtacgacca
      841 taatcaaaac gtgctagcgg acgctccaca tatcgataag aaagttcact gatgattaga
      901 atcaatgtga tttccactaa agtatgcaac aaaacatgat cggcaatatc agtcaccttt
      961 gcttcataaa aaatcataac tggaaactga tacagataaa tgccatagct tcgtttgcca
     1021 atccaagtaa atactggatt tgtcagccat cgattcaaac ttgctccggg gtgtgctgtc
     1081 actgcaacta gcactacgca taacagactc actaggagca ttcctccgta atagatgaaa
     1141 ttgtaatgat catctaaaaa gaagaagcaa agaatcagtg ccaaaagcga gattcctcca
     1201 gcgatattca gaatattttt cgcttgtttc gggatgtttt tctttaaacg cgtacttggc
     1261 caaacaaacg caagcgcact tcccaaccat atagaaaaca gacgagtgtc ggtaccgtaa
     1321 taaacacggg ttggatcgcc tcctggtgta tacaagatca tcatcaagat ggcggagagg
     1381 aaggatgtac ctaacacagt ataaaagatc cattttcttt tacgaacaaa taccatcaac
     1441 aatacaaaca agatcggcca aaccaaatag ttttgacctt ctactgctaa agaccagata
     1501 tgcgtaaacg gtgactcatt agcaaatcga tcgaaataag ataaaccgtt attgatttgc
     1561 caccagttgt tgacatacag taagctgctg accacaactc ctcgaagatt gtttaacagc
     1621 cctcgctgaa acaaagtgat ataagccgaa gctgtcacaa gcataaaaac aagtgccgga
     1681 tagagtctct tcattcttct gacataaaat tgccaaatat tgatttttcc attttgttcc
     1741 cattcctgcc gcagcagatc cgtgatcaaa taaccagata cgacgaaaaa aatcggaacg
     1801 cccaaatagc cgcctttcat actagttggc agaaggtgat agaagataac acccaccaca
     1861 gctaatgaac ggagcccatc aaacccagtg atgtagcggc tgttttttaa ccttttttgt
     1921 ttttccattc aatattccaa cttttcataa acttttttca ttcgtaatat acgggtatta
     1981 agtgaaaata gcaagtagaa tcgtgtcctt tctctactat ttttttctat catcccataa
     2041 atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata aaaaagcagt
     2101 ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac gtctgtctac
     2161 agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat agaaacgacc
     2221 aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag ctttcattgc
     2281 ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg tctgcttatc
     2341 atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca tacagttttg
     2401 gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca tcttcttttg
     2461 tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg tactgtaaaa
     2521 catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat tgaagtagat
     2581 cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca cgatatctag
     2641 accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca tcacagcccc
     2701 cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta aaatatggat
     2761 acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct tcccgatttt
     2821 taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg ataccggtac
     2881 ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac cagtaatacc
     2941 acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg cgatcaacgc
     3001 ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat tttcgaatgt
     3061 ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt gtttttcaat
     3121 taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt ttatcggcta
     3181 ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg aaaccatact
     3241 tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa tgacttcacg
     3301 aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg caataccgcc
     3361 atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt cttccatttg
     3421 ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag tatgaatacg
     3481 gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt ctgcatagac
     3541 ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg tgaatggatg
     3601 gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc aatcagttac
     3661 ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta aacgtgtccg
     3721 gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca tcagcaagtc
     3781 acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca tgaatttagc
     3841 aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct tagctccgaa
     3901 ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat ctgcagcgcc
     3961 tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga agactttgaa
     4021 ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac gtgtatccgg
     4081 tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa atggcaatgt
     4141 gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg tgtaagtttg
     4201 gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt caggctgacg
     4261 gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac ggtcgaatcc
     4321 agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat agaaatgtcc
     4381 agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag atttccctag
     4441 gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa tcgcttttgt
     4501 tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat ccaaataacg
     4561 atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa atggcggggt
     4621 ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac cagtggccat
     4681 tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt cgatcacgta
     4741 ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag gattaaagac
     4801 gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc caaggtctcg
     4861 tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt cggaaactaa
     4921 tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa taaagtcatt
     4981 tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact tttttctttt
     5041 cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc accgatcacc
     5101 aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc tttacggttc
     5161 atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac tttcaatgct
     5221 tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt aaatgcaggg
     5281 ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat tgcaaatccg
     5341 aagccaggta ttgaaggacc acctagttct tccactagat tgtcataacg tccgcctgcg
     5401 caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa aatcgtatgt
     5461 gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac agccaatgca
     5521 tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag ataatctaag
     5581 atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc caatacgcgt
     5641 aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc aaaaggtaaa
     5701 agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc taatgaattg
     5761 atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc catcaccatg
     5821 ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat ttgatggaat
     5881 tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat ataaaaagtt
     5941 ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga acgaacgatt
     6001 ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata aaaatcatac
     6061 atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac ttcatagtgt
     6121 tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa acgtgctgtt
     6181 tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt tccttttggt
     6241 ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa aatcaccctt
     6301 gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt ttgaaagatt
     6361 tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg tccatctcca
     6421 gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc ctctcttgta
     6481 aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata acctactaaa
     6541 aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg ttttagttca
     6601 gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg gcagaaataa
     6661 gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat gccgttggag
     6721 gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt cgtacagctt
     6781 tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc cgttccgaag
     6841 aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc gtcatcgcta
     6901 caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct gagtttctca
     6961 aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac attttttata
     7021 tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg cacgaattaa
     7081 aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa caattgaaag
     7141 aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg atccatcggc
     7201 aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt gttccctatt
     7261 ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa aaacaatttg
     7321 gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc agagaaattt
     7381 taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact cctgcgcaac
     7441 ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct ggagctaagc
     7501 gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc aatgaagatt
     7561 atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc aatttatagt
     7621 aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata agtttatgct
     7681 aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact gattgaatgg
     7741 aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct gatcggaaaa
     7801 cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt gtcgaaaatt
     7861 tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt ttgctttccc
     7921 tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat catggtctgt
     7981 tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat ctacttctac
     8041 ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc tcatttcttg
     8101 aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca aatctggtct
     8161 actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg ttggacggat
     8221 gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat ctaaatctgt
     8281 ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat tttgtcgagc
     8341 ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga caccttttat
     8401 gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt ctgctaattt
     8461 ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc aaatagatgt
     8521 aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg cttcaagtgc
     8581 ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg gtgtcattag
     8641 atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct gtactccacc
     8701 ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt tgaaattcct
     8761 tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt ctttattgaa
     8821 aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca acatgttgta
     8881 tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg gtacttcttt
     8941 catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac tttttttccg
     9001 actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta gagtttatca
     9061 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
     9121 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
     9181 gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc attgaatgat
     9241 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
     9301 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
     9361 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
     9421 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
     9481 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
     9541 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
     9601 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
     9661 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
     9721 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
     9781 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
     9841 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
     9901 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
     9961 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
    10021 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
    10081 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
    10141 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
    10201 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
    10261 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
    10321 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
    10381 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
    10441 atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
    10501 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
    10561 tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac taaagatatc
    10621 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
    10681 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
    10741 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
    10801 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
    10861 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
    10921 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
    10981 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
    11041 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
    11101 cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
    11161 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
    11221 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
    11281 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
    11341 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
    11401 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
    11461 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
    11521 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
    11581 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
    11641 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
    11701 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
    11761 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
    11821 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
    11881 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
    11941 taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt cgatactctt
    12001 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
    12061 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
    12121 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
    12181 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
    12241 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
    12301 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
    12361 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
    12421 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
    12481 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
    12541 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
    12601 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
    12661 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
    12721 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
    12781 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
    12841 gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc ttctcttcaa
    12901 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
    12961 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
    13021 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
    13081 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
    13141 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
    13201 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
    13261 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
    13321 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
    13381 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
    13441 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
    13501 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
    13561 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
    13621 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
    13681 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
    13741 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
    13801 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
    13861 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
    13921 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
    13981 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
    14041 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
    14101 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
    14161 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
    14221 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
    14281 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
    14341 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
    14401 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
    14461 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
    14521 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
    14581 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
    14641 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
    14701 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
    14761 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
    14821 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
    14881 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
    14941 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
    15001 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
    15061 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
    15121 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
    15181 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
    15241 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
    15301 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
    15361 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
    15421 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
    15481 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
    15541 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
    15601 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
    15661 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
    15721 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
    15781 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
    15841 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
    15901 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
    15961 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
    16021 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
    16081 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
    16141 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
    16201 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
    16261 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
    16321 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
    16381 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
    16441 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
    16501 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
    16561 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
    16621 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
    16681 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
    16741 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
    16801 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
    16861 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
    16921 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
    16981 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
    17041 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
    17101 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
    17161 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
    17221 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
    17281 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
    17341 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
    17401 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
    17461 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
    17521 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
    17581 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
    17641 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
    17701 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
    17761 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
    17821 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
    17881 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
    17941 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
    18001 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
    18061 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
    18121 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
    18181 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
    18241 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
    18301 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
    18361 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
    18421 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
    18481 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
    18541 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
    18601 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
    18661 cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
    18721 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
    18781 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
    18841 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
    18901 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
    18961 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
    19021 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    19081 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    19141 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    19201 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    19261 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    19321 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    19381 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    19441 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    19501 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    19561 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    19621 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    19681 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    19741 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    19801 gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    19861 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    19921 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    19981 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    20041 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    20101 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    20161 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    20221 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    20281 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    20341 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    20401 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    20461 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    20521 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    20581 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    20641 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    20701 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    20761 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    20821 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    20881 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    20941 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    21001 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    21061 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    21121 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    21181 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    21241 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    21301 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    21361 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    21421 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    21481 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    21541 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    21601 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    21661 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    21721 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    21781 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    21841 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    21901 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    21961 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    22021 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    22081 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    22141 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    22201 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    22261 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    22321 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    22381 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    22441 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    22501 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    22561 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc agtttttgat
    22621 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    22681 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    22741 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    22801 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    22861 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    22921 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    22981 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    23041 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    23101 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    23161 tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    23221 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    23281 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    23341 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    23401 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    23461 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    23521 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    23581 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    23641 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    23701 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    23761 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    23821 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    23881 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    23941 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    24001 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    24061 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    24121 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    24181 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    24241 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    24301 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    24361 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    24421 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    24481 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    24541 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    24601 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    24661 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    24721 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    24781 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    24841 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    24901 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    24961 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    25021 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    25081 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    25141 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    25201 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    25261 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    25321 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    25381 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    25441 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    25501 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    25561 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    25621 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    25681 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    25741 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    25801 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    25861 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    25921 tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    25981 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    26041 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    26101 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    26161 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    26221 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    26281 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    26341 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    26401 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    26461 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    26521 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    26581 caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta tcttgggagg
    26641 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    26701 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    26761 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    26821 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    26881 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    26941 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    27001 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    27061 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    27121 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    27181 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    27241 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    27301 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    27361 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    27421 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    27481 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    27541 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    27601 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    27661 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    27721 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    27781 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    27841 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    27901 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    27961 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    28021 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    28081 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    28141 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    28201 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    28261 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    28321 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    28381 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    28441 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    28501 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    28561 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    28621 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    28681 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    28741 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    28801 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    28861 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    28921 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    28981 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    29041 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    29101 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    29161 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct ccgcagagtt
    29221 tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc gttcatccgt
    29281 cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat ggccgcatac
    29341 caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat cagccactga
    29401 ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac cgcagggtct
    29461 gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac taaccaatta
    29521 cgatggttta catctccgtg tactactgat aagttttcct tatctattga cgggatctgc
    29581 tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc ttccggtaat
    29641 tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc gatacgggtc
    29701 agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat cacgtcgttt
    29761 ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg tgctgtcaaa
    29821 gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc ttcccttgaa
    29881 agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc atcacgcatg
    29941 cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc ttggtctaac
    30001 tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta cttattattc
    30061 tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta attgccattc
    30121 aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt caacgctcta
    30181 tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc ttcttcaaac
    30241 gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc gctagcggaa
    30301 cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata accgacgcga
    30361 taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc ttatggattg
    30421 gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac agttggaaac
    30481 cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg actactccgg
    30541 caatcaataa cccacctata attactaatc ggaaataaag accactaaaa tcagaacctc
    30601 ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt ttaatatgag
    30661 tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca ggaacatctg
    30721 tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca tgcaccattt
    30781 tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat agacttgcct
    30841 gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga aacaaaaaga
    30901 tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga tctagtcgaa
    30961 acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat atgatcagat
    31021 agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc atggccaatc
    31081 cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa gctcgttcca
    31141 aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct gcctctgcaa
    31201 aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt atgagccctc
    31261 caattgcaaa cggtggctgc agggttttca accaattaga ataatataat cccacaccgc
    31321 caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc atatatttca
    31381 tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg tgcctgctcc
    31441 tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa ttcttttctc
    31501 aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac aaaacgatca
    31561 caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc tccttctttt
    31621 ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa gcctaaaaat
    31681 ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat catcactttc
    31741 tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga taaccgaaaa
    31801 gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc gtaggccatc
    31861 gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg agtctctgga
    31921 atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag atgattgatt
    31981 tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt cgtacttttc
    32041 cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc gaaactgata
    32101 tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat taaactcatt
    32161 ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac ggtattttgt
    32221 ttttttcctt acaatagctt taacttatgg taaagttaag ctgaagtatt tattctttat
    32281 agaaaggatg ttttatatgg aaaactgtat tttttgcaaa atcatcaatc aagaaatccc
    32341 aagctataaa atatatgaag acgacaaggt ctatgctttt ttagacatct ctcaagcaac
    32401 gaaaggacac acattagttg tgcctaaacg tcatgtagca gatatttttg aatatgatcc
    32461 cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat tggaaaaagc
    32521 ttttccagaa atgaaaggtc ttaatatctt gaataacaac cgtgaactag cctatcaatc
    32581 tgtttttcat tcacatatcc atttagtccc tcgctacagc aaagaggatg atttttctat
    32641 tcactttgtc aatcatcaag atagttatgg atcggaagaa ctaaaagcta ttcaagaaac
    32701 aatcgtaaag caggtgtcct gcgatgatta aaaacttttt caaaggactg ttatttggag
    32761 ctgctgctgg cactgtcgga ggtctgttat tggcgcccag gagcgggaat gaaacaagaa
    32821 agaaactgat cgaagaattg gaagaagcaa caactctgac aaatgattta aataacagcc
    32881 tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac agctgaatct gtgattcctg
    32941 ctttccaaga agcaatcaaa aaagatatcg aagattttaa attccaagcc gaaccacgta
    33001 tcacacaaat caatgaacaa gtagaaaaac taaaagctga tctgccagag tatcctgaac
    33061 aaatataata atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt
    33121 atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt
    33181 aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta caggagtgca
    33241 aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg ctgttttaac
    33301 tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag gcggaacgat
    33361 cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat ccttagtaca
    33421 acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa ccgataagca
    33481 agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg aatcacaatt
    33541 agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca atttagcttt
    33601 tgaagctggc ttgaaagctc acgtagatat tacagatgac tatttgaaaa ctgcttggaa
    33661 atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag atgaagcaaa
    33721 agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca aagacaaatc
    33781 aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa cgacaacaac
    33841 tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga tctccgatgt
    33901 tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca aaatggtgaa
    33961 aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag atatcgctac
    34021 tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag aattaaaaga
    34081 tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat tcacaacaac
    34141 ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt ctgacacaaa
    34201 atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat caaaataaaa
    34261 gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg ttttgaatga aaaaaataaa
    34321 tttttgctat aaatcttttg gtacatagaa acttcggttg tccataccga aaatccgatc
    34381 ggtgtatttt cctggttctg tttgacgaat cgaagtcaac atcatctgca tcgaagcatc
    34441 gatattgtcg atttgatgta atatttctgc ttccattatt cttggacgta caggtgagcc
    34501 atattctaat aagccatggt gtgctaaaac catatgacgt aatacgagaa catcttcgtc
    34561 agcttcgctg attttcaacg caagacaggc tttagttatt tcttcgtcaa tcaataccag
    34621 atgaccgatc aaatttcctg caagcgtata ttctgtcgac ataggaccgg aaagttcaat
    34681 cacctttcct aaatcatgca aaatgatacc agcatacaac aatgctcgat tcagctcagg
    34741 atattcatcg gcgattgatt tccctaagtg aagcattgtc aaagtatgat aagctaatcc
    34801 actggcaaat gcatgatggt ttctttttgc tgccggaaac tcaaaaaatt gtttttgata
    34861 tttattaagt agatagcgaa caatacgatt ccaatgcgca ttggtgatct caaacaacaa
    34921 ttgattgatt tcttcttcca tttcttcttt cttcaaaggg gctctttcca tgtataaact
    34981 aggctctcct ggctcatcta cccttgttaa gcgcatatga agaatcttta cttgagggtt
    35041 tccttgataa acctctcttt taccgtttag aaaaacgact gtgccggctg taaatttatt
    35101 gatttcctcc tccgaagcat cccagtattt accatcgata gtacctgaag tatcttggaa
    35161 agtaaaagca ataaattttt ttccgttttt agcgattctt acatctgcac ttttgatcag
    35221 gacaaattgt tcaaactgct catcgattac taaatcacga atttttttca ttatttgctc
    35281 cttctaattg ttttacgatt tggttcatct catggtaata gcttaccatt tctttatcag
    35341 aagaaaaaca aattatttga tacttttctc caaatttagc taaaagttca gctaattgat
    35401 actttcgctg atgatcataa tgaagccagc catcatcaat gatgatcggg catatcgaac
    35461 ggttagcttc taatgagagg taagcaaatc tcatcgccat aatcagctga tctttcgtac
    35521 ctgtagataa ctcatagatg ggaaattctg tatatccgtc tgttaaagtc agtgtatcat
    35581 ttttcagtaa tatcttctga taatttcctt ttgttaaaat acctaaataa tcacctgctg
    35641 ctcttaacaa ctggggtaat tgtctttcac tcatctctgt tgaaagatca cctaagaatg
    35701 tcccggcgat ttcaagtgct ccccattcct ccagtaattc ctgtaatttt gctttttgcc
    35761 ggctagcttg ttgataaagt tcgtccaatg tgccgtctct ttgcaactct tctacttgaa
    35821 gttggagtcg ttgccgattt tcacttgctt ctttttcttc ttgctggagt tgggattgct
    35881 tttctttcag ttggcgcagt cttacagata atttctcttt tgtcagctct tcaggaaata
    35941 gtggagtaag catttccgat aattcattgt atcttttcat ctgctgttcg gcttgtttgg
    36001 ctttttgtaa aaagacaggt acttccgaag gataagataa gcctgcttga gtcaattgtt
    36061 cgctgtattc ttgtatccat actttttgtt tctttttcaa ttgattgatt tcttgcttca
    36121 ataaagtatt ttgctgatag cttctggcaa atttcatctg ttccatctct tgtataaaac
    36181 gggaaagcaa taccattttt tctgaaattg tcttatcttg tatcggtaac cattctttaa
    36241 caaactcaaa tttttggtcg atttgtcgta gtttttcttg tatttcctgt tctcttttat
    36301 gtttccgctc aaatacgcca tataatttct gataatctat gacttcctgt ccatattctt
    36361 tcatgagttt gagcgtatcc atctttccta agtgccgatg atccgcaaaa cgatatactt
    36421 cttgctgaag aatctgtttt tcttctccag ttctcttttg tttttcttct gcttcagcta
    36481 attgagctaa ggctagatca agttgtccta gtttttcttg ccacattcct tttatctcag
    36541 agaaatcatt tttagtttta atttttggtt gccttttgta gaagaaatag ccaatcatgc
    36601 ctgcaccaat tagtaaaagg atcactttca agggcattgg tacaaatata cctgcaatga
    36661 ttattgctcc gccagccatc agcatatacg gtttagatga agagactggt gctatctctg
    36721 actgattttg taataatgct ggataggtct cttccaagtc attcacttct gcttcaatca
    36781 tctcttgctg ttctttagct atttggagac gcacagaaat ctggctttct agatcagcaa
    36841 tccttctttc ttcagccaga agatgcttcc attcttcttc atataaaaat tcttccggta
    36901 acggattttt ctgccaatgc cacctttgtt ccaaaatctt caattctgaa tttgtttctc
    36961 tagtctcaat ttgtaatctt tgccattctt cgtttaattg ttccgctgta aattgaagtt
    37021 gctggagatc ttttagttcc tgctcctgct ctaaataaaa ataatatctg gctgactgct
    37081 gatccatccc gctatgcttt tccagtgttg cttctaattc ttccaaacgc tcatttgttt
    37141 gctgataccg tttggagaaa gactccagat ctaaaagaag ttgctcatca ggaagacctg
    37201 tagtgctgtt ttttttgaga gattgccatt cttcatataa agaaaaatta agttcttgct
    37261 gtctggctaa tgaaaattgt ttgttcgcct cttttaattg tgccaataac tgctgctgtt
    37321 gcgactcgta ttcctgttct tgttggatca acttgccaaa ataagcttcc tgttcttgtt
    37381 tttgatgaat tttttcttgc agctctttcc attcatttaa tttttgattg attagtaatt
    37441 tttggccttt tttcttgaat aattgttgtg ctttctgata atattcttgc cgcttttgga
    37501 atacttgaga actgcccgtg atgcctaaag aaatcagcgc atcatgtagc tctttttctc
    37561 tcaaatgatc tagttccatc aactgctctt gctggaaagt aaatacctgc tggaataact
    37621 ctttcgtcaa aggtgcgatc agtttttcta gtaactcatc acttcccact tgatccccca
    37681 gccacacctt cgattttccg cgattttgct gcttgtagcg ctcgacagca aattcaccat
    37741 atacaggatg tttaagccat attttgccgc cataggctga gccgtctttt ggtgtataat
    37801 cctttttttt acggcctttt gaagggaaac caaataatat tgcttgaata aattgataaa
    37861 tcgttgattt tcctgcttcg tttcccccat agatcagctg attgcctgaa taaaagtcaa
    37921 aacttttttg acgccatttt ccaaaagcag caatctcgat ccttaaaatc ttcattcctg
    37981 atcctcctca aataaaaaat cttgattaag cagctgcttg gctttttcaa gtgtttcttc
    38041 ttgatattcc ggcagttcgc tcaatattct agccgcttct tgatgactat atacttcatt
    38101 taaaatttgt tggaaatctt gaggtgtctg atagtattga aataactgtt ccatcaattt
    38161 agcagaagca gcaagtgggg tcttcgtata aaaagtattc tctatgagag aaatacgcca
    38221 aataaagaaa tcatttatcc tatctgagaa ttcctctaac aaatagtcta atagctctcc
    38281 tgattgtatc cgttccaaaa catctgtatt gagatggtca taatctttta attttatctc
    38341 aattaaacta aatccatcct ctacttttga aagctgctga cgaatgtgaa caatgacctc
    38401 ttttgtcgtt cttgctttcc tcaatgatat ttcctttgtc ttccaataga cctctgctac
    38461 tttaatcgga tgtatttggc aacttccttt acttaattca actagtagaa tagatgtaga
    38521 agcttgttcc tttttagtat gaccttgagg cgcacctgga taaacaattg tttgcttttc
    38581 atttaagacc gttggcacat ggatatgccc taaagcccaa taatcgtatc ccttttcttg
    38641 catctttgag ggttggaaag gagcatagtt tcctttttga gagacgccag gttccccgtg
    38701 gtacaaccca atatggtaat ctgtgagttc tctagaagga aattccatca ctttgtcttt
    38761 ttgaatccac ggttgacgat aactaaatcc actgagagag acagtttctc cactttttat
    38821 cgttattttt tttgtctcta ccgtttcgga tgtaaataaa tggacatttt taggaaagga
    38881 gaaccaatac cgctcttttt gatagaaatc atgatttccg aatattataa atacaggtat
    38941 atggttcttt tctaaccgtt tcatctgttc tgaaaaatgt ttctgtattt ttaatgaagg
    39001 acgattttga tgaaaattat ctcctgcaag taaaacaaaa tcaacgttat ttattattgc
    39061 ctgttcaatg atatttgata aaacggtcag attcgtcttc aatagttttt cctgtacagt
    39121 tttatcaaga gttgtcagtc cttcaaatga ccgatccata tgtaaatctg ccgcatggat
    39181 aaatttcacc attaaaaatt tcctcctttt tttcttttat cataccattt tcaatgtttt
    39241 ttttcatcaa taaccgaaaa tttgttcgta ttatgcaaag aaattacgat aaatggccga
    39301 cttcaaaaaa taagtttatt cgaaaaaaag attgtgacat gatttttgtc acaatcctaa
    39361 atatctatgt tatattcgct tcgtttgaca ctgaagaagt actatttgta ctggaattta
    39421 tgctccattt ttccgtattt agaattctat gttctatact accttaatta gtttatatgg
    39481 taagaattct ttcaaatgct tgagatactc cgtctttatc atttgaaaca gtgacataat
    39541 cagctttttt catgatttct tgatgactat ttcccattgc aaaactaata tctgcacttt
    39601 caaacataga taagtcattt aaactatctc cgatcgcgat tctcaaaggt ttctttaaat
    39661 tgtagattga ttccaaacga tcaaacacca cacctttaga tacattttga ggtacgattt
    39721 caatatttac aggtgccgat tcagtaacag acacatttag catctttttc aaatggttaa
    39781 atatattagc tttcaattga cgattaggaa taaaaataaa aaatttgtaa atgctgatag
    39841 catccttttc tataatttcc tcaatattat tataagggat tgaacaaata gatttttcat
    39901 actcaagctc atagtttagc tctttgatag aaaattcact ccctacctcg tgaaaaattt
    39961 ttttcatttt atttttataa ttctgagatt cataacttcc gagatttgta tatattttgg
    40021 ttggaaattt cgataggaca gaatcaattt tttttacatc atttttatcg aaggaagact
    40081 caaatatctt tttttctcca tcaaatccaa cagaaccatt taaccctact gctggtatcc
    40141 ttaaaccaga ttttttgaga atattattaa tgtcttctac ttctcttcca gaacaaatga
    40201 atggaaaaca gtcttccggt aaatgtttca ttgttcttgc atttttttga ctaatgctct
    40261 ggtcagaatt caaaagagta ccgtctaaat ctataccaat tagtttcatt tcatccatgt
    40321 ctcctatacg ttgattataa attcactgct tattgtaagt ctaaatttct cacaaaacat
    40381 atactaatta tacctaattt aagtaagtac aaacatcatt ttaagaatag gaaaggacat
    40441 ttatatacta ttctctgtat ttttgattta taaataaata tgaattgaat ccaaatcagc
    40501 tatattacta ttttataata accaactata aaaaacgaag ttaggtgaaa aactaacttc
    40561 gatattcata aaagactcta ttagttcaac ttagcaaaag atttaaacaa aaaaagatga
    40621 actatttcta gttcatcttt ttgcctt
//
LOCUS       DACIWG010000026        39397 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.026, whole genome shotgun sequence.
ACCESSION   DACIWG010000026 DACIWG010000000
VERSION     DACIWG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.026"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            176..574
                     /locus_tag="IYF54_001373"
     CDS             176..574
                     /locus_tag="IYF54_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ9831710.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            811..1353
                     /locus_tag="IYF54_001374"
     CDS             811..1353
                     /locus_tag="IYF54_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ9831711.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="IYF54_001375"
     CDS             complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="IYF54_001375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9831712.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2139..2366)
                     /locus_tag="IYF54_001376"
     CDS             complement(2139..2366)
                     /locus_tag="IYF54_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ9831713.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="IYF54_001377"
     CDS             complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="IYF54_001377"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9831714.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2901..3614)
                     /locus_tag="IYF54_001378"
     CDS             complement(2901..3614)
                     /locus_tag="IYF54_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ9831715.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3617..4513)
                     /locus_tag="IYF54_001379"
     CDS             complement(3617..4513)
                     /locus_tag="IYF54_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9831716.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4671..5513)
                     /locus_tag="IYF54_001380"
     CDS             complement(4671..5513)
                     /locus_tag="IYF54_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9831717.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5645..6298
                     /locus_tag="IYF54_001381"
     CDS             5645..6298
                     /locus_tag="IYF54_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ9831718.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6361..6888)
                     /locus_tag="IYF54_001382"
     CDS             complement(6361..6888)
                     /locus_tag="IYF54_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9831719.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6908..7855)
                     /locus_tag="IYF54_001383"
     CDS             complement(6908..7855)
                     /locus_tag="IYF54_001383"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ9831720.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7867..9753)
                     /locus_tag="IYF54_001384"
     CDS             complement(7867..9753)
                     /locus_tag="IYF54_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9831721.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(9913..11991)
                     /locus_tag="IYF54_001385"
     CDS             complement(9913..11991)
                     /locus_tag="IYF54_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9831722.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(12004..12279)
                     /locus_tag="IYF54_001386"
     CDS             complement(12004..12279)
                     /locus_tag="IYF54_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831723.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12641..13096)
                     /locus_tag="IYF54_001387"
     CDS             complement(12641..13096)
                     /locus_tag="IYF54_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831724.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13250..14458)
                     /locus_tag="IYF54_001388"
     CDS             complement(13250..14458)
                     /locus_tag="IYF54_001388"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ9831725.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14570..14902)
                     /locus_tag="IYF54_001389"
     CDS             complement(14570..14902)
                     /locus_tag="IYF54_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ9831726.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            15050..15925
                     /locus_tag="IYF54_001390"
     CDS             15050..15925
                     /locus_tag="IYF54_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9831727.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(16007..16588)
                     /locus_tag="IYF54_001391"
     CDS             complement(16007..16588)
                     /locus_tag="IYF54_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ9831728.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16594..17853)
                     /locus_tag="IYF54_001392"
     CDS             complement(16594..17853)
                     /locus_tag="IYF54_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ9831729.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17855..18901)
                     /locus_tag="IYF54_001393"
     CDS             complement(17855..18901)
                     /locus_tag="IYF54_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831730.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19136..19411)
                     /locus_tag="IYF54_001394"
     CDS             complement(19136..19411)
                     /locus_tag="IYF54_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ9831731.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19654..20964)
                     /locus_tag="IYF54_001395"
     CDS             complement(19654..20964)
                     /locus_tag="IYF54_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ9831732.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="IYF54_001396"
     CDS             complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="IYF54_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ9831733.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22510..23190)
                     /locus_tag="IYF54_001397"
     CDS             complement(22510..23190)
                     /locus_tag="IYF54_001397"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ9831734.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23239..23874)
                     /locus_tag="IYF54_001398"
     CDS             complement(23239..23874)
                     /locus_tag="IYF54_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9831735.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23940..25343)
                     /locus_tag="IYF54_001399"
     CDS             complement(23940..25343)
                     /locus_tag="IYF54_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ9831736.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25330..26361)
                     /locus_tag="IYF54_001400"
     CDS             complement(25330..26361)
                     /locus_tag="IYF54_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831737.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26392..26613
                     /locus_tag="IYF54_001401"
     CDS             26392..26613
                     /locus_tag="IYF54_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ9831738.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26778..27515)
                     /locus_tag="IYF54_001402"
     CDS             complement(26778..27515)
                     /locus_tag="IYF54_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ9831739.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27668..29008)
                     /locus_tag="IYF54_001403"
     CDS             complement(27668..29008)
                     /locus_tag="IYF54_001403"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ9831740.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29245..30768)
                     /locus_tag="IYF54_001404"
     CDS             complement(29245..30768)
                     /locus_tag="IYF54_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ9831741.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31147..31758)
                     /locus_tag="IYF54_001405"
     CDS             complement(31147..31758)
                     /locus_tag="IYF54_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9831742.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31881..32006)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(32088..32804)
                     /locus_tag="IYF54_001406"
     CDS             complement(32088..32804)
                     /locus_tag="IYF54_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ9831743.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32810..33379)
                     /gene="scpB"
                     /locus_tag="IYF54_001407"
     CDS             complement(32810..33379)
                     /gene="scpB"
                     /locus_tag="IYF54_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ9831744.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33363..34160)
                     /locus_tag="IYF54_001408"
     CDS             complement(33363..34160)
                     /locus_tag="IYF54_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ9831745.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34130..34501)
                     /locus_tag="IYF54_001409"
     CDS             complement(34130..34501)
                     /locus_tag="IYF54_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ9831746.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34520..35407)
                     /gene="xerD"
                     /locus_tag="IYF54_001410"
     CDS             complement(34520..35407)
                     /gene="xerD"
                     /locus_tag="IYF54_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ9831747.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35503..35976)
                     /locus_tag="IYF54_001411"
     CDS             complement(35503..35976)
                     /locus_tag="IYF54_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ9831748.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(36081..36944)
                     /locus_tag="IYF54_001412"
     CDS             complement(36081..36944)
                     /locus_tag="IYF54_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ9831749.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(37140..39239)
                     /locus_tag="IYF54_001413"
     CDS             complement(37140..39239)
                     /locus_tag="IYF54_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ9831750.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        10882 a         8199 c         6678 g        13638 t
ORIGIN      
        1 taaagaaatt ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt
       61 cacttttttg atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac
      121 agcgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
      181 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
      241 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg
      301 ggatgacact ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga
      361 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
      421 tgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
      481 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
      541 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
      601 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc
      661 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
      721 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
      781 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
      841 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
      901 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
      961 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
     1021 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
     1081 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
     1141 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
     1201 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc
     1261 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
     1321 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
     1381 tttggattaa acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact
     1441 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
     1501 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
     1561 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
     1621 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
     1681 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
     1741 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
     1801 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
     1861 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
     1921 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
     1981 ttgtcaatct ctatcaatcc atccctcttt ttttcattct ttctttatta taagaaaaat
     2041 atcaaaaaaa tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga
     2101 caaaaaactt ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct
     2161 aaatattttt cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt
     2221 tccagataat tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta
     2281 ccggcttctg tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt
     2341 aaataatgaa aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag
     2401 cccggcgtgc atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt
     2461 cttcttctgc tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga
     2521 aacgtccact ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg
     2581 tcaaataaaa gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt
     2641 cagttggatc agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg
     2701 gatcatagat aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat
     2761 aagtcggatc tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa
     2821 gcgtgtcgaa aggctggatc atacaccaga agcagcctcc tgcaaatatt gctcgttcca
     2881 ttatgattcc ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt
     2941 tcagtactgc tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct
     3001 tttggcaaat atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta
     3061 atatacatcc cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct
     3121 gtattgactt cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct
     3181 ttgattggca aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg
     3241 ttttccatca catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg
     3301 aaagtaccat tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct
     3361 ttttgatact cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg
     3421 atcgttaaga caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt
     3481 tcccgattag caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga
     3541 aggataagga aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca
     3601 gatcttgttt tcatgtttat tgatcatcct ccttctctaa ccattggttt tgtgtctcga
     3661 tcagctgatc tctcactgca tttgccatca attgataacc taaattattt ggatggaaat
     3721 gatcttcttc atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg
     3781 tttgatctgt tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat
     3841 cattgattgg gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt
     3901 tccaattgtt aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat
     3961 tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata
     4021 gttctgtgac attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc
     4081 cgaataaatt attttggata actttcatca aatcattacc gccaacagtc agtgtgataa
     4141 aatcagctgt tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct
     4201 ggtcacttcg ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac
     4261 ggtcttttag atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta
     4321 ctccttcagt cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat
     4381 cttctctcac agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta
     4441 aacctgcata gatgatgatc gcaattaata atgggaacaa aacgaggatc aaatgtcgtt
     4501 tcgttaattt cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt
     4561 atcaaaaagg tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa
     4621 aagagaataa gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca
     4681 taatacatga ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc
     4741 agtacaggga tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc
     4801 aattcttttg catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt
     4861 tcatttttca aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca
     4921 gggattaatt cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc
     4981 aaaccagttg ttcggctgat tcttccacct tttaccaagt tatctagtgt ggatattccg
     5041 atgtataatt ttgtattgtc ttgtactttt ttgacttctt caaggatttt ctctactgtt
     5101 ttaccttctt ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg
     5161 gtatcactgt cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct
     5221 tctactgttc cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct
     5281 cctagctcat catacatcga gatgaattct ccaattggtg gttggcttgt tttgggcaga
     5341 ttttttgctt tcgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga
     5401 tatactgttc cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt
     5461 acattttttt ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc
     5521 tctctctctt tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct
     5581 atttttagta taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt
     5641 tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc
     5701 agtcacccat ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa
     5761 aggtgcccac ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat
     5821 gattctttta tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa
     5881 ggtgttccaa gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc
     5941 tttttgtttg ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt
     6001 gtttgcgatt cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc
     6061 aaaagtatct acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc
     6121 tctttatcac tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta
     6181 cacgataggc gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca
     6241 tttgtttgtt atgttaggtg caggttttat gtacttctct gttttattct ttacttaata
     6301 aaaaaaataa agctatggat gggacagctc taaataacag ctagtctatc catagctttt
     6361 ttatatcgtc aattcgcctg tcccgctttt tacaggattc tcattacgct gatacgtttc
     6421 aaattgatgc ggataacgat ttttttcatc catttctcct tgactagtat tgatcagtga
     6481 ccaatcagac cagtcaacag gtggaaaata agcatctcca tcgaatacat gatggatgac
     6541 tgtacggtat aatatcgtac aaaatggcag aaattcttga taaacagctg accctccagc
     6601 gatgaaaaag ataccgtctg tttctttttc cagagcaagc acgtcttcca cactatgcat
     6661 gacatgcacg ccttctgctt tataggaaga atctctcgtc aaaacaatcg tctgacgatt
     6721 cggcaacgga cggctaccca tcccttcgaa tgtttttcgt cccatcacta gtgtatttgc
     6781 ttctgtcatt tgtttgaaga atttcagatc atttggcaaa cgccaaggaa gacggtcttc
     6841 ttttccaatc aatccgtttt catcctgtgc ccaaattgca gctaacattt tctcatcctt
     6901 tctgattcta aacagcgata ggagctttga tcgccggatg tgggtcatat ccttctaatt
     6961 ggatatcttc catctcaaaa tcaaatactg actgcttttc ctgattcaaa actaaagtcg
     7021 gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg ttcgatatga ttgatataaa
     7081 gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt tcatgtgcaa
     7141 ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg aacgcctaga aacacatccc
     7201 cactgcgttg atacaattgg cagcttagcc gtccctcttg aacataaaat tgaaacatcg
     7261 tatgacaagg aggcaaagcc atgctaggaa catcttccgg attccatgca gaaacgatca
     7321 atcttctaga atcaggagtc tttttgattg cttcgatcac ttgtttgatt tgatcgataa
     7381 agccaccgtc ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt ccaagatcgc
     7441 cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc ttgctctgct
     7501 ttttatattc ttcattgaat tcaggatctg tcaaacatct atggccaaaa tcagtcatat
     7561 ctggtccatt atagtctgga cttttgatgt agcgttcaaa tgcccattca tcccaaatat
     7621 ggtttttatt ttgtaataga tagcggatat tcgtatctcc ttttaaaaac caaagcaatt
     7681 cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac cctttttgca
     7741 gatcaaaccg catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt ctgtcttctt
     7801 tacgatgccc ttcttccaac aattttcttc ctaagtcaag atacgcttgt tccatccttt
     7861 ttcctcctaa tcttctaccc attcactcaa gtattcccac cgtgccattt tttcttcaag
     7921 ttgtgcttct gtttcagata cgtcgttttg caattcttgt aatcgagtga aattatctcc
     7981 ttgatgattc atctcttctt ggagcaatga gattttttct tcaagttctg caatctcatc
     8041 ttcaatcgtt tcccattctt tttgttccat gtaagataat ttcttctttt cagcaggttc
     8101 cttgctctgt atttttggtt tcgctttttc agcaggctgc tgggtctctt tttgcttcag
     8161 taaataactg gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt ggaatactaa
     8221 taacttatct gctaccttgt ccaagaaata acggtcatgg gaaacagtga tcacagcacc
     8281 tggaaaactt ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca aatcattggt
     8341 cggttcatcc aataataaaa cattaggttg ctgaatcagc agtttcaaca aaaataaccg
     8401 gcgtttttct ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc ttggaaataa
     8461 aaatcgttca agtaattctg ctactccgat ttgactgcca tctgctgttt ttgcttcttc
     8521 agcagcttct tgtaagtaag caatcatccg tttatctggt gccatttctt cattttcttg
     8581 tgtataatac gcaagatgaa ccgtttcacc gatagacatt gtaccgctat ccaggggaat
     8641 tctgccagct agaatattaa gcaatgtcga ttttcccgca ccatttttac cagtaatacc
     8701 tagtctttcc cgagattgaa tcaataaatc taaatgttct agcagtgttt ggtcatttat
     8761 acggtaactg ccatctttga tttccagtac ttttttccct aaacgttgtg tcgctaggtt
     8821 cagctctaaa tcatcttctt ggtttacttg gtacaagttc tcctttagat cttcgaaacg
     8881 gttgatccgt gcttgctgct tggtgccacg tgcctgtacg ccagccctca tccaagctaa
     8941 ttcctgttta taaagccgct tgcgcttttc ttcttgttct acttctacac gatcacgttc
     9001 tgctttttct aagacgtaag cttcataatt tcctttatac tcgtataatt ttccaaatga
     9061 aagttcgaag attcggtttg ctactctatc tagaaaataa cgatcgtggg taaccatcaa
     9121 tagtgcgcca cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt cataatctaa
     9181 atggtttgtt ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca cttgcgccag
     9241 actaactcgt tttttctgac cacctgataa agtactgatt tttttatcta attcactgat
     9301 tcctaatttt tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat cttctttatt
     9361 catttgttct tctgctttag cataacgttt ttgcacatca gggtcttctc cgttttcccc
     9421 tagttcgatc aatgcttcct catatgctcg aacagtttgg atcaaggggc tgtttccttg
     9481 aaagactgct tggagaacag ttaattcatc ggaaaattca gctgcttgag aaagataacc
     9541 aatacggtaa tcagttggat aaaacactgt ttgccgatca ccgtcgctgc tatcgatacc
     9601 ggcaataata tttaagagac ttgttttacc cgtcccgttc gtcccaatca acccgatacg
     9661 gtctttttcg tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac cataagtttt
     9721 tttgaggtca gttactttta attctttcaa catgttccct ccttatcttg gatattgtat
     9781 cataaaaata gtgaacttca ggaaaatttt tttggtattt gttctttttg tgttctcaaa
     9841 aaaagaacag gaccgattcg atcctgttct gatatgtttt gttttttata aaacttgaaa
     9901 atttttacct ttttatttta ttggactcgt tttttttgac tttgctcgaa acgcaaatcg
     9961 gaaagaaaag ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct
    10021 gcgatagctg ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt catcaatgat
    10081 ttttctttca agataataac ctcttagcat ttttttcgta aacagactat cttgctggat
    10141 catccacgaa gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg
    10201 gataaaaaat tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat
    10261 aatcaactga gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat
    10321 aaatgtctga cgatcagcat atcttctttg ccaaaacatt ttcggatgga gaacgattcg
    10381 cattaaccta cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat
    10441 agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt
    10501 gcccgaacga taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat
    10561 ttcttgtact tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg
    10621 ttcttgataa ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat
    10681 ttcctttatt tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa
    10741 atccgtgctt ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac
    10801 caaagtcact aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat
    10861 catattattg attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac
    10921 accgccaaat gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc
    10981 tttgaataca taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat
    11041 taatacgata atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga
    11101 gaacacttgc gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc
    11161 aatagttgtc catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt
    11221 cctaaaacca gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc
    11281 aaatgcctct gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc
    11341 tgtgacatct tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac
    11401 aagcaaaaag ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt
    11461 tactgctgaa aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc
    11521 agatgcatca ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt
    11581 cactcgtcca gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa
    11641 agcaaagcag gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc
    11701 gacaagggtc aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag
    11761 aatcgaagaa atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac
    11821 taagaacaat tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac
    11881 tccaaggaaa atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt
    11941 cgataaggtg atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc
    12001 cctttatttt ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt
    12061 ggtttcgact acttcaacct gtgtttggtt cccctctaat tcacactgta cacgttttga
    12121 ctccaaacga agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct
    12181 tttacgcaac ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc
    12241 atgcaacagt tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca
    12301 aacttccatc attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt
    12361 tattgctagt tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc
    12421 agatatcaag taaaacttaa gaaagataaa agtttctgac aaaaggatgt ttactttccc
    12481 attcctgcct ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg
    12541 tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga
    12601 cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt
    12661 atacatcttc tacacgttga tctggattga tcacgatata caagttacct gttttttcgc
    12721 tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga
    12781 agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt
    12841 atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg
    12901 caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa
    12961 caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc
    13021 cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg
    13081 tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca
    13141 gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct
    13201 gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt
    13261 tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga
    13321 cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt
    13381 cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat
    13441 cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt
    13501 ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag
    13561 tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac
    13621 acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt tggctgatca
    13681 ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta
    13741 aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct gtttcaataa
    13801 atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta
    13861 tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt
    13921 cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg
    13981 catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctagat
    14041 cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga
    14101 tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg
    14161 gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat
    14221 cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa
    14281 gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt
    14341 tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct gcattctcta
    14401 attttttcag aactggaatt gccttttggt attccagagg taattgtttt aatttcattt
    14461 ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca tggtgactgt
    14521 ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt tactcgtcct
    14581 tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt
    14641 cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa
    14701 gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt
    14761 ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg
    14821 gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt
    14881 ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg
    14941 caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt aggaaggaat
    15001 ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg
    15061 aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct
    15121 tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga tcaccttgat
    15181 cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca acatcccatt
    15241 gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg ttctaggtac
    15301 actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca
    15361 gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat
    15421 cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga ttttagaaca
    15481 aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc
    15541 ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt
    15601 cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt
    15661 ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac
    15721 tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt
    15781 tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt
    15841 cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca
    15901 gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc
    15961 cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa ttgttatgtt
    16021 cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag
    16081 ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa
    16141 gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat
    16201 cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat
    16261 attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat
    16321 tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca
    16381 atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat
    16441 tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca
    16501 agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat
    16561 tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa
    16621 tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc
    16681 tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc
    16741 atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa
    16801 ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg
    16861 attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat tacttaaagt
    16921 aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc
    16981 tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg
    17041 gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc
    17101 ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat taacttctcg
    17161 aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc
    17221 tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtacgat
    17281 agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat
    17341 tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt
    17401 gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg
    17461 aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg
    17521 gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt
    17581 ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg
    17641 gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc
    17701 ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg
    17761 gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa
    17821 agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga
    17881 tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt tggtatccgt
    17941 ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa
    18001 ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca
    18061 agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc
    18121 acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat
    18181 tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct
    18241 ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt
    18301 gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc
    18361 cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg
    18421 tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta
    18481 gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga
    18541 tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga
    18601 tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga
    18661 aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc
    18721 agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat
    18781 agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga
    18841 ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca
    18901 tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat
    18961 aagagcaaag ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt
    19021 ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg
    19081 aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt
    19141 taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt
    19201 cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa
    19261 agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg
    19321 aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa
    19381 ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata
    19441 tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat
    19501 tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt
    19561 taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc
    19621 ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg
    19681 gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata
    19741 agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc
    19801 gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat
    19861 tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt
    19921 catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga
    19981 aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa
    20041 atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga cgataataat
    20101 tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc
    20161 ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat
    20221 cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc
    20281 tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt
    20341 cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga
    20401 ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc
    20461 ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc
    20521 atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc
    20581 gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt tattacttct
    20641 gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac
    20701 acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat
    20761 aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa
    20821 ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga
    20881 gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac
    20941 gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag
    21001 tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat
    21061 tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc
    21121 atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta
    21181 cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg
    21241 gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag
    21301 ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca
    21361 cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag
    21421 agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca
    21481 aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc
    21541 aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag
    21601 acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat
    21661 gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg
    21721 cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg
    21781 aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga
    21841 tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat
    21901 agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta
    21961 caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct
    22021 tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt
    22081 ttgaaagtag atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat
    22141 ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt
    22201 cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt
    22261 cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt
    22321 tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac
    22381 ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat
    22441 tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt
    22501 tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac
    22561 gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag
    22621 cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt
    22681 ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc
    22741 acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat
    22801 gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa
    22861 ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg
    22921 acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg
    22981 cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga
    23041 tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata
    23101 aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg
    23161 cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc
    23221 cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt
    23281 gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc
    23341 cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga
    23401 agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact
    23461 tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg
    23521 agacacagta gattcttccg cgattgtact agattctact gttgaagtct cagttgactc
    23581 ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc
    23641 tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc
    23701 gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg
    23761 ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca
    23821 tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt
    23881 acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat
    23941 caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca
    24001 gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa
    24061 caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg
    24121 cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc
    24181 tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc
    24241 catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa ataaagctca
    24301 aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca
    24361 ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct
    24421 tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat
    24481 ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca
    24541 aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact
    24601 gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa
    24661 atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc
    24721 gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt
    24781 ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt
    24841 agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct
    24901 ggacggaaat caactcccca ttgagacacg caatgagcct catctactac atataacgca
    24961 atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa
    25021 aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt
    25081 aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct
    25141 tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc
    25201 aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca
    25261 tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat
    25321 ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata
    25381 caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc
    25441 tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc
    25501 gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct
    25561 ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt
    25621 ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca
    25681 aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg
    25741 cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg
    25801 ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa
    25861 cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca attggctttc
    25921 actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc
    25981 ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact
    26041 agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc
    26101 ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca
    26161 accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa
    26221 actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg
    26281 ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag
    26341 cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta
    26401 ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca
    26461 gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat
    26521 caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca
    26581 gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca
    26641 ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg
    26701 acttattttg aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc
    26761 tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc
    26821 acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag
    26881 ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa
    26941 tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc
    27001 gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg
    27061 aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt
    27121 acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc
    27181 ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact
    27241 gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga
    27301 gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc tccttcaaaa
    27361 aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt
    27421 tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg
    27481 actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt
    27541 gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac
    27601 tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg
    27661 tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat
    27721 acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga
    27781 acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata
    27841 caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta
    27901 actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc
    27961 catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag
    28021 ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt
    28081 tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg
    28141 cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa
    28201 gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac
    28261 ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca
    28321 atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat
    28381 tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt
    28441 gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg
    28501 taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg
    28561 taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga
    28621 cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc
    28681 agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg
    28741 gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca atctctgaat
    28801 aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca
    28861 atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg
    28921 cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat
    28981 gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc
    29041 atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc
    29101 atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct
    29161 attgacttat ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga
    29221 ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat
    29281 ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa
    29341 acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa
    29401 accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc
    29461 cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa
    29521 tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact
    29581 taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac
    29641 ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc
    29701 taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga
    29761 cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct
    29821 tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa
    29881 aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa
    29941 agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc
    30001 gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt
    30061 tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa
    30121 caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc
    30181 ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga
    30241 ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg
    30301 gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa
    30361 tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga atttcttcac
    30421 aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc
    30481 aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc
    30541 cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg
    30601 attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata
    30661 gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac
    30721 cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct
    30781 ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca
    30841 gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta
    30901 ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag cgtttccttt
    30961 tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt tgcaaagaat
    31021 tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca
    31081 ttattgcatg tcttgtattt ttttccaaaa aaaaaagacc aagaaatttc ttgatctttt
    31141 tgatatttat ttgactgctg tatgacgttg ttcgaactga tgttgttttc gacttaacca
    31201 tggaagaagt tgcgcatgca gcactaaaaa gacagcactc acgatgaatc ctttgatcaa
    31261 gttgaaagga acgatcccaa tcagtacata gtttgctaaa gacatgccca gcatctgtgt
    31321 tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata acgaaataat ttgcgatact
    31381 cataaaaata gttagagcga tggttcctgt aatgacacct aaaaatttgt ttcttccttt
    31441 atgctgtttc tttacactaa agaaataata aatcgggaat gtaaagatcg ttgttgataa
    31501 gaaactagca gtgtctccta ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa
    31561 tactgaacgc aagaacgctg ttgcaactcc ggccgctgga ccgaataaga acatgctgat
    31621 caataccgag atatcactaa aatcgatctt caaaaaacta aaagccggca tgatcggaaa
    31681 agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa ccattttctg
    31741 tacacgggtg ttcttcattt gaaatcctcc tgttaggact cctcttcagc gaactttttg
    31801 acgtttctct ccaaatgaaa aaactctatt ttccagggaa aatagagtta gagttatttg
    31861 atcaagcgtc gtcaaataag ctctatcttc tttatccaga ctatactgtc ggtatcggaa
    31921 tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg actataacca ccggtcggga
    31981 atttcaccct gcccctgaag acgaacctta tataattttt gtttacatat tcattataca
    32041 tatctattca aaaaacgcaa catctaacac ttataaaaag taagtgttca ttttgagtgg
    32101 ttcagcaaac tgctgacttc ttttttgttc agttcacggt aatccccagg acgtaggcct
    32161 tgtaaagtca gatctccgta tttttcacgt tttagttttt gcacaggaag accaactgct
    32221 tgaagcatat ttttcacttg atgattacgt ccttcgtgga tcgtcaattc cacgatactg
    32281 tggtttgtct taggatccac tgataaaatt tggaatttag ctggagatgt ttttcgtcct
    32341 tcgatcttta ttccttttgt caaaggtgca agcatcgatt ttgtagcgat cccttttacc
    32401 tttgcaacgt aaactttatc cacttcatgt tttggatgtg tcaggcgctg agcaaaatct
    32461 ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc tacaggatag
    32521 atacgttctt ttaccatcgg caaaagatcc gtaacaactt ttcttccttt gtcatctgca
    32581 acagcagaaa taactccttt aggtttgtac aataaatagt aaccgtattc ttcttgataa
    32641 attgggactt catccacttc cacagtgtca tgtttcccaa cttttatccc taattccctt
    32701 acaacttgtc cgtttacttt tacacgtcct gcaaggatga attcttctgc ttttcgacga
    32761 gaagtgatgc cagcgtgggc aattactttc tgcaatctct ccattacaat tattctcctt
    32821 tattcattcc atcaaagaat agatctggtg acacgtcttc ttcaagtgaa atttccattt
    32881 cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa ataatctaag aagtagccag
    32941 ttgttccata taatatcggc cgacctggtc cttctactct acctttttct tcaatcagtt
    33001 tatgggcagc tagacgctgt accgctcctg aaacctgaac tcctcgaatc tgctcgattt
    33061 cagctcttgt gattggctgt ttataagcga tgattgctaa ggtttcgacg gcggcttgag
    33121 ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa gggagccagc tcttttttag
    33181 tcgttaatac ataatggctt tccgtttcaa aaatatgcaa agctgagtgc gagttttgag
    33241 tgtaagattc ttttaattct ttcactaaac ttgtgacttc tgcttcgctg agatttaaca
    33301 gtccgcttaa ttcttcatta ctgatccctt cctcgccaac aataaaaaga agggcttcaa
    33361 gttcacttaa tttcgtcatg ttcggtatct cctactgctg gataaagcat aattgattca
    33421 tagttttctt tttgttgcgc aatgatctgc ccttttttca tcaattctag caaagccata
    33481 aaagtagtga caacttcact tttagaaggt gactcaaaaa aagaatcgaa agtacagcca
    33541 tgcgcatttc tttttatacg ctcttcgata aataccattc gttcttcgat cgttgtgtca
    33601 tcgggcgtga tcgttttttc cactttttga gaaaatttct tacgttctaa gattttctgc
    33661 atggcacgga tcaaatcgat ttttttgact tgtcctcgct tcaacggctt ttcagcatcc
    33721 gctaacccat cgatgttcat aggttctttt gtgtaataaa gacttcgttc ctctgctttt
    33781 tctgtcagtc tttcagctgc atatttatat ttacggtatt ctaaaagctg agtgaccaat
    33841 tcttctcgag gatcttcttc ccataattct tcatcgtctg tgacgaattc ttggacagga
    33901 agcaatgtct tgcttttgat tgccatcaat gtagcagcca tcactaagta ttctcctgca
    33961 acagctagct cgagtgtttt cattgtatgg atataattca tatattgctc cgtgatcgta
    34021 gcgatcggta tatcatatat atcgatttcc atttgctgaa tcagatgaag cagcagatcg
    34081 agaggaccct cgaatacatc taatttaata ttgatctccg tcaactcatt cactctttct
    34141 tctttttctt tgctagccat tttccaatgt aaggctctgt gatttcagtt gcacggatgg
    34201 agacatccgg gtacttttcc tgtaattcat ctaacatttg aaagcccata ttctcaccgc
    34261 gataagacgg attcaaagaa atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa
    34321 taccaataat cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa agttcataat
    34381 cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga agttttacct gtcagtttct
    34441 tatggaaact caatagtccc atagcaattt tacggttttc tttccggtaa tgtgttaaca
    34501 tcttctcacc ttcctatgac taagcccttg gaaaatgctg cttataaaca tccgtcattc
    34561 ttttcttcgt gatatgtgta taaatctgag tcgtagagat atccgcgtga cctaacaatt
    34621 cctgtactgt ccgcaaatca gctccatttt ctagcagatg tgtggcaaag ctatgccgca
    34681 gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg tactaactgc ttcagatttt
    34741 tccagattcc ttgccgagaa agacctttcc catgattatt gacaaataca tgcatttcag
    34801 atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta gcgttccagc cattggatcg
    34861 cataatcacc taaaggaatg atccgctctt tatctccttt tccaactgtc tgtaccagtc
    34921 caagagaaag atgaagatct cccaatttca atccaatcaa ttcactgacg cgcatcccag
    34981 tagcatacat gacttccaag attgcacgat ctcttatccc taacgttttt gtcgtatcag
    35041 gtgcttcaat cagcttttct acttctgtca gagtcaatgt acttggtaat ttctgtgctt
    35101 tcttcggcgt atcgatatgt tgcatcggat catgatcagt gatacgctct tgccgtaaaa
    35161 actgatggaa tcttctgaga cttgagatca tccgagtaac agttgctgtc gctttttttt
    35221 cttcatgcaa cttctccaga aaacggatga ctaaaaagcg atcgacatcc tgccatgaag
    35281 caattttctg ctcctgtaga aaatgcaagt actgttccaa atcacgttca tagctttgtc
    35341 gtgtattggc ggacaatccc cgttcaatat tcaagtagtg gagataatcg ttgatttgct
    35401 ctttcataat aatccccctc atgcctctct atcatatcag ataccgcttc attctgatag
    35461 tctttttcca taatctgagc aaagaaaaag ccgtctagac actcagacgg actggtttct
    35521 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
    35581 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
    35641 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
    35701 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
    35761 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
    35821 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
    35881 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
    35941 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
    36001 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
    36061 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
    36121 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
    36181 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
    36241 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
    36301 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
    36361 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
    36421 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
    36481 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
    36541 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
    36601 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
    36661 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
    36721 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
    36781 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
    36841 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
    36901 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
    36961 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
    37021 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
    37081 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
    37141 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
    37201 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
    37261 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
    37321 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
    37381 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
    37441 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
    37501 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
    37561 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
    37621 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
    37681 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
    37741 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
    37801 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
    37861 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
    37921 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
    37981 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
    38041 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
    38101 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
    38161 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
    38221 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
    38281 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
    38341 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
    38401 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
    38461 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
    38521 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
    38581 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
    38641 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
    38701 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
    38761 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
    38821 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
    38881 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
    38941 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
    39001 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
    39061 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
    39121 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
    39181 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
    39241 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
    39301 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
    39361 tagaaaaaaa agcttgagac tgcgtagtct caagctt
//
LOCUS       DACIWG010000027        37213 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.027, whole genome shotgun sequence.
ACCESSION   DACIWG010000027 DACIWG010000000
VERSION     DACIWG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.027"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            647..964
                     /locus_tag="IYF54_001414"
     CDS             647..964
                     /locus_tag="IYF54_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ9831751.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IYF54_001415"
     CDS             complement(1055..1420)
                     /locus_tag="IYF54_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831752.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IYF54_001416"
     CDS             1628..2110
                     /locus_tag="IYF54_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831753.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IYF54_001417"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IYF54_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ9831754.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IYF54_001418"
     CDS             complement(3484..4758)
                     /locus_tag="IYF54_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9831755.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IYF54_001419"
     CDS             5031..7502
                     /locus_tag="IYF54_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9831756.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IYF54_001420"
     CDS             complement(7605..9341)
                     /locus_tag="IYF54_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ9831757.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IYF54_001421"
     CDS             complement(9338..10042)
                     /locus_tag="IYF54_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831758.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IYF54_001422"
     CDS             10173..10676
                     /locus_tag="IYF54_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ9831759.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IYF54_001423"
     CDS             10636..10974
                     /locus_tag="IYF54_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ9831760.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IYF54_001424"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IYF54_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ9831761.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IYF54_001425"
     CDS             12525..13103
                     /locus_tag="IYF54_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ9831762.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IYF54_001426"
     CDS             13107..13628
                     /locus_tag="IYF54_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9831763.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IYF54_001427"
     CDS             complement(13707..14261)
                     /locus_tag="IYF54_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9831764.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IYF54_001428"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IYF54_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ9831765.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IYF54_001429"
     CDS             14801..15052
                     /locus_tag="IYF54_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ9831766.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IYF54_001430"
     CDS             15177..15968
                     /locus_tag="IYF54_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831767.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IYF54_001431"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IYF54_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ9831768.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IYF54_001432"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IYF54_001432"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ9831769.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IYF54_001433"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IYF54_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ9831770.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="IYF54_001434"
     CDS             18151..18444
                     /locus_tag="IYF54_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ9831771.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            19287..21614
                     /locus_tag="IYF54_001435"
     CDS             19287..21614
                     /locus_tag="IYF54_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9831772.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21740..22069
                     /locus_tag="IYF54_001436"
     CDS             21740..22069
                     /locus_tag="IYF54_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ9831773.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            22109..23113
                     /locus_tag="IYF54_001437"
     CDS             22109..23113
                     /locus_tag="IYF54_001437"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ9831774.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            23110..24539
                     /locus_tag="IYF54_001438"
                     /pseudo
     CDS             23110..24539
                     /locus_tag="IYF54_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            24745..25917
                     /locus_tag="IYF54_001439"
     CDS             24745..25917
                     /locus_tag="IYF54_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9831775.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(26079..26534)
                     /locus_tag="IYF54_001440"
     CDS             complement(26079..26534)
                     /locus_tag="IYF54_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831776.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            27124..27546
                     /locus_tag="IYF54_001441"
     CDS             27124..27546
                     /locus_tag="IYF54_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9831777.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(27712..28905)
                     /locus_tag="IYF54_001442"
     CDS             complement(27712..28905)
                     /locus_tag="IYF54_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9831778.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            29129..29506
                     /locus_tag="IYF54_001443"
     CDS             29129..29506
                     /locus_tag="IYF54_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9831779.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            29494..29832
                     /locus_tag="IYF54_001444"
     CDS             29494..29832
                     /locus_tag="IYF54_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9831780.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29966..30409
                     /locus_tag="IYF54_001445"
     CDS             29966..30409
                     /locus_tag="IYF54_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ9831781.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            30671..31486
                     /locus_tag="IYF54_001446"
     CDS             30671..31486
                     /locus_tag="IYF54_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9831782.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            31496..32158
                     /locus_tag="IYF54_001447"
     CDS             31496..32158
                     /locus_tag="IYF54_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ9831783.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            32363..34027
                     /gene="alsS"
                     /locus_tag="IYF54_001448"
     CDS             32363..34027
                     /gene="alsS"
                     /locus_tag="IYF54_001448"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ9831784.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            34040..34750
                     /gene="budA"
                     /locus_tag="IYF54_001449"
     CDS             34040..34750
                     /gene="budA"
                     /locus_tag="IYF54_001449"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ9831785.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            34880..35377
                     /locus_tag="IYF54_001450"
     CDS             34880..35377
                     /locus_tag="IYF54_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ9831786.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            35562..35822
                     /locus_tag="IYF54_001451"
     CDS             35562..35822
                     /locus_tag="IYF54_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831787.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(36184..36423)
                     /locus_tag="IYF54_001452"
     CDS             complement(36184..36423)
                     /locus_tag="IYF54_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831788.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            36709..37023
                     /locus_tag="IYF54_001453"
     CDS             36709..37023
                     /locus_tag="IYF54_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831789.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT        12510 a         6482 c         7379 g        10842 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattta aaaataaaat
    19141 ctttgtatta aaggacaaga ctaaaataac cttagaataa aaagagaaaa aaagccccaa
    19201 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
    19261 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
    19321 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
    19381 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
    19441 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
    19501 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
    19561 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
    19621 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
    19681 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
    19741 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
    19801 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
    19861 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
    19921 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
    19981 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
    20041 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
    20101 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
    20161 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
    20221 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
    20281 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
    20341 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
    20401 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
    20461 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
    20521 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
    20581 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
    20641 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
    20701 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
    20761 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
    20821 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
    20881 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
    20941 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
    21001 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
    21061 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
    21121 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
    21181 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
    21241 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
    21301 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
    21361 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
    21421 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
    21481 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
    21541 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
    21601 gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
    21661 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
    21721 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
    21781 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
    21841 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
    21901 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
    21961 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
    22021 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
    22081 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
    22141 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
    22201 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
    22261 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
    22321 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
    22381 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
    22441 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
    22501 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
    22561 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
    22621 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
    22681 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
    22741 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
    22801 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
    22861 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
    22921 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
    22981 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
    23041 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
    23101 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
    23161 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
    23221 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
    23281 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
    23341 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
    23401 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
    23461 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
    23521 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
    23581 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
    23641 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
    23701 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
    23761 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
    23821 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
    23881 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
    23941 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
    24001 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
    24061 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
    24121 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
    24181 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
    24241 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
    24301 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
    24361 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
    24421 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
    24481 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
    24541 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
    24601 tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag gaggaactat
    24661 tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc tcttcatata
    24721 catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact ggacatcttc
    24781 atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt agaagattca
    24841 ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc atcgaataag
    24901 aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca acagttggca
    24961 gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc aaaagcatta
    25021 aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat cgaaatccca
    25081 aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt gcaaaaagga
    25141 acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca tgccgttctt
    25201 tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa
    25261 ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt agaaaaaatc
    25321 caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag agatctcgtg
    25381 actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt gacggggcat
    25441 cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa
    25501 aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg cttagtttgg
    25561 ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc tttatctttt
    25621 tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc
    25681 aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc tgatgaatac
    25741 ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca tgaggagctc
    25801 ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa attgaaaaaa
    25861 ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa agaatgaatg
    25921 aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag ctgacgaaat
    25981 ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta tttttttatg
    26041 tttcagaaga attttattat tatagtataa cttttgaatt agtttccata acatttggag
    26101 caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata cgtaccattt
    26161 ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt ttgttcatta
    26221 ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc tgcttgctgt
    26281 cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt
    26341 cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa
    26401 tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct
    26461 gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat
    26521 tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct
    26581 tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc ggagatcgtg
    26641 gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt agtagcacca
    26701 cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt cactaaattt
    26761 cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt ttacttactt
    26821 gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc cgttttgata
    26881 cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata tgtacgttat
    26941 tttattatta gattattgac atttatataa taaacgatta aatgattcat atcaatagaa
    27001 tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt gtgacaatat
    27061 gatatttgta tccgtttttt ttcttgtatg tagtatagta aagtagatac aggaggagaa
    27121 aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat tcatttactg
    27181 cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa ggtatcaagt
    27241 aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata tcctgataag
    27301 gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga gaacttccca
    27361 atctacatta tttatcttca tttctacaga aaatcatata attatcattt gattgaattc
    27421 atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata tacgagtgta
    27481 tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac ggacgtcaaa
    27541 gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct gcatcaagta
    27601 atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt cttattaccg
    27661 cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact atcaatgctg
    27721 caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca gactgactag
    27781 gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca tcaagaaacc
    27841 gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga aattattcgc
    27901 cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc caccatacat
    27961 gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact ggctatgggc
    28021 taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa aggctagttt
    28081 aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca tgcctaaaat
    28141 agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat tttgcataat
    28201 tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta ccatgatact
    28261 aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa gtttggtttt
    28321 cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc gacgaatgaa
    28381 caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac caaacgttgg
    28441 aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac caataccagc
    28501 aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca aggacataca
    28561 cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat aaaccaagta
    28621 gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga ccgtttgaga
    28681 aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac ctccaatgag
    28741 catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa tatgaaaaga
    28801 agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct ctaaagcaat
    28861 tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta tacccctttt
    28921 cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa acaagacttt
    28981 tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt ttaatagaaa
    29041 tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat aagtaaagtg
    29101 gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg aagaaaaggg
    29161 catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac cagttaatcg
    29221 gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg actttacgat
    29281 ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc gcctatctag
    29341 cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa gtgaactttt
    29401 gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa aaatcaagaa
    29461 atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa atcaaggagc
    29521 atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga aggaacaatc
    29581 tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca actgaaggag
    29641 ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat gatcttgaca
    29701 gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa cgaatcaaac
    29761 cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta cgatcggctg
    29821 tgtggaagct gataattcga atacattggc actattacta gaaaagttga tccgtgatta
    29881 tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg agtgactaga
    29941 aaaaagcctg ttagatttag cgattttgct agatccagta gatacagaga aatacgaaac
    30001 gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt ctgttttagc
    30061 aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga tctcaaagcg
    30121 tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc agttgctgat
    30181 cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc aggtaggtgc
    30241 tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat tcgttccgct
    30301 tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga ttttatcacc
    30361 tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat aagtatctct
    30421 aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt gtattttttc
    30481 atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc gcttacaaac
    30541 ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt aatcattaca
    30601 aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag caaaaattcg
    30661 ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt
    30721 attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt
    30781 gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat
    30841 ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat
    30901 cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta aactaaattt
    30961 taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc
    31021 ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata cgttgtggtc
    31081 gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatactacag
    31141 tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag agtggggaat
    31201 ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac
    31261 attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa
    31321 taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt
    31381 aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca
    31441 aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa
    31501 agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt
    31561 ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt
    31621 gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg
    31681 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
    31741 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
    31801 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
    31861 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
    31921 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
    31981 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
    32041 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
    32101 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
    32161 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
    32221 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
    32281 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
    32341 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
    32401 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
    32461 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
    32521 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
    32581 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
    32641 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
    32701 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
    32761 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
    32821 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
    32881 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
    32941 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
    33001 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
    33061 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
    33121 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
    33181 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
    33241 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
    33301 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
    33361 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
    33421 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
    33481 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
    33541 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
    33601 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
    33661 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
    33721 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
    33781 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
    33841 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
    33901 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
    33961 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
    34021 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
    34081 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
    34141 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
    34201 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
    34261 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
    34321 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
    34381 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
    34441 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
    34501 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
    34561 tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
    34621 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
    34681 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
    34741 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
    34801 ttttgaggag aaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
    34861 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
    34921 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
    34981 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
    35041 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
    35101 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
    35161 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
    35221 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
    35281 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
    35341 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
    35401 aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt tttcggtttt
    35461 tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt taatgttatt
    35521 atcattttaa ataatgaaaa aacattaagg atggatgaaa tatgaataac gaaactatca
    35581 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg attggagaag
    35641 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
    35701 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
    35761 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
    35821 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
    35881 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
    35941 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
    36001 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
    36061 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
    36121 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
    36181 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
    36241 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
    36301 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
    36361 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
    36421 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
    36481 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
    36541 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
    36601 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
    36661 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt ggatttttac
    36721 agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt ttttgataag
    36781 agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga aaaaaagtgg
    36841 gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact ttcttcaaca
    36901 ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat tattggaatc
    36961 cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc aaaaaataat
    37021 taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta tagcgaatgt
    37081 gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat tttatgttag
    37141 gatgaattaa tttctctaga gtttataatt cgccgtttgt ataattaagc gagacaaata
    37201 aaaaagccat tta
//
LOCUS       DACIWG010000028        37102 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.028, whole genome shotgun sequence.
ACCESSION   DACIWG010000028 DACIWG010000000
VERSION     DACIWG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.028"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(160..444)
                     /locus_tag="IYF54_001454"
     CDS             complement(160..444)
                     /locus_tag="IYF54_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831790.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="IYF54_001455"
     CDS             complement(462..1352)
                     /locus_tag="IYF54_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ9831791.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="IYF54_001456"
     CDS             complement(1372..1590)
                     /locus_tag="IYF54_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831792.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="IYF54_001457"
     CDS             complement(1560..3260)
                     /locus_tag="IYF54_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ9831793.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="IYF54_001458"
     CDS             3527..4300
                     /locus_tag="IYF54_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831794.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IYF54_001459"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IYF54_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ9831795.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="IYF54_001460"
     CDS             complement(5124..6095)
                     /locus_tag="IYF54_001460"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ9831796.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IYF54_001461"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IYF54_001461"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ9831797.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IYF54_001462"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IYF54_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ9831798.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="IYF54_001463"
     CDS             complement(9050..9874)
                     /locus_tag="IYF54_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ9831799.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="IYF54_001464"
     CDS             complement(9847..10527)
                     /locus_tag="IYF54_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831800.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="IYF54_001465"
     CDS             complement(10550..11488)
                     /locus_tag="IYF54_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ9831801.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /locus_tag="IYF54_001466"
     CDS             complement(11733..12584)
                     /locus_tag="IYF54_001466"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ9831802.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IYF54_001467"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IYF54_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ9831803.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="IYF54_001468"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="IYF54_001468"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ9831804.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IYF54_001469"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IYF54_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ9831805.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IYF54_001470"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IYF54_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ9831806.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="IYF54_001471"
     CDS             complement(19462..20703)
                     /locus_tag="IYF54_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ9831807.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IYF54_001472"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IYF54_001472"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ9831808.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22614)
                     /locus_tag="IYF54_001473"
     CDS             complement(21730..22614)
                     /locus_tag="IYF54_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9831809.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="IYF54_001474"
     CDS             complement(23392..24678)
                     /locus_tag="IYF54_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ9831810.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="IYF54_001475"
     CDS             complement(24671..25939)
                     /locus_tag="IYF54_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ9831811.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="IYF54_001476"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="IYF54_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ9831812.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="IYF54_001477"
     CDS             complement(26711..27157)
                     /locus_tag="IYF54_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831813.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="IYF54_001478"
     CDS             complement(27250..27495)
                     /locus_tag="IYF54_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831814.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="IYF54_001479"
     CDS             complement(27610..28188)
                     /locus_tag="IYF54_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9831815.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="IYF54_001480"
     CDS             complement(28265..28795)
                     /locus_tag="IYF54_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ9831816.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IYF54_001481"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IYF54_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ9831817.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="IYF54_001482"
     CDS             complement(29652..30047)
                     /locus_tag="IYF54_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ9831818.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="IYF54_001483"
     CDS             complement(30044..31456)
                     /locus_tag="IYF54_001483"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ9831819.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IYF54_001484"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IYF54_001484"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ9831820.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32311..33771)
                     /locus_tag="IYF54_001485"
     CDS             complement(32311..33771)
                     /locus_tag="IYF54_001485"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ9831821.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMDWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="IYF54_001486"
     CDS             complement(33941..35098)
                     /locus_tag="IYF54_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ9831822.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IYF54_001487"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IYF54_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ9831823.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="IYF54_001488"
     CDS             complement(36565..37092)
                     /locus_tag="IYF54_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ9831824.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10250 a         8090 c         6505 g        12257 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agtcaggaaa cgccagaatt gagcattgat cttcgccaac aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggatcg gcatgaaaag atttttccct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt ttacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttaaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatc cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atctaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 aaactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgattaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ct
//
LOCUS       DACIWG010000029        36570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.029, whole genome shotgun sequence.
ACCESSION   DACIWG010000029 DACIWG010000000
VERSION     DACIWG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.029"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IYF54_001489"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IYF54_001489"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ9831825.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IYF54_001490"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IYF54_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ9831826.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IYF54_001491"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IYF54_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ9831827.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IYF54_001492"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IYF54_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ9831828.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IYF54_001493"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IYF54_001493"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ9831829.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IYF54_001494"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IYF54_001494"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ9831830.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IYF54_001495"
     CDS             complement(8054..8524)
                     /locus_tag="IYF54_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ9831831.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IYF54_001496"
     CDS             complement(8570..8995)
                     /locus_tag="IYF54_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ9831832.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IYF54_001497"
     CDS             complement(9062..9331)
                     /locus_tag="IYF54_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ9831833.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IYF54_001498"
     CDS             complement(9342..10937)
                     /locus_tag="IYF54_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ9831834.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IYF54_001499"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IYF54_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ9831835.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IYF54_001500"
     CDS             complement(14490..15050)
                     /locus_tag="IYF54_001500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ9831836.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IYF54_001501"
     CDS             15403..16377
                     /locus_tag="IYF54_001501"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ9831837.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IYF54_001502"
     CDS             16547..17947
                     /locus_tag="IYF54_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ9831838.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IYF54_001503"
     CDS             complement(18025..18747)
                     /locus_tag="IYF54_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ9831839.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IYF54_001504"
     CDS             complement(18826..19332)
                     /locus_tag="IYF54_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ9831840.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IYF54_001505"
     CDS             19455..21254
                     /locus_tag="IYF54_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ9831841.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IYF54_001506"
     CDS             complement(21322..22896)
                     /locus_tag="IYF54_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831842.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IYF54_001507"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IYF54_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ9831843.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IYF54_001508"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IYF54_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ9831844.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IYF54_001509"
     CDS             complement(24673..25311)
                     /locus_tag="IYF54_001509"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ9831845.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IYF54_001510"
     CDS             complement(25312..25962)
                     /locus_tag="IYF54_001510"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ9831846.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IYF54_001511"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IYF54_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ9831847.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IYF54_001512"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IYF54_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ9831848.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IYF54_001513"
     CDS             complement(29105..29845)
                     /locus_tag="IYF54_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ9831849.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IYF54_001514"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IYF54_001514"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ9831850.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IYF54_001515"
     CDS             complement(31216..32154)
                     /locus_tag="IYF54_001515"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ9831851.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IYF54_001516"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IYF54_001516"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ9831852.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IYF54_001517"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IYF54_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ9831853.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IYF54_001518"
     CDS             complement(35314..35619)
                     /locus_tag="IYF54_001518"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ9831854.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IYF54_001519"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IYF54_001519"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ9831855.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DACIWG010000030        35388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.030, whole genome shotgun sequence.
ACCESSION   DACIWG010000030 DACIWG010000000
VERSION     DACIWG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.030"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(3..1529)
                     /locus_tag="IYF54_001520"
     CDS             complement(3..1529)
                     /locus_tag="IYF54_001520"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ9831856.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1544..2191)
                     /locus_tag="IYF54_001521"
     CDS             complement(1544..2191)
                     /locus_tag="IYF54_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ9831857.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2375..2704)
                     /locus_tag="IYF54_001522"
     CDS             complement(2375..2704)
                     /locus_tag="IYF54_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ9831858.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2881..3609
                     /gene="sfsA"
                     /locus_tag="IYF54_001523"
     CDS             2881..3609
                     /gene="sfsA"
                     /locus_tag="IYF54_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ9831859.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3625..4638
                     /locus_tag="IYF54_001524"
     CDS             3625..4638
                     /locus_tag="IYF54_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9831860.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4638..5915
                     /locus_tag="IYF54_001525"
     CDS             4638..5915
                     /locus_tag="IYF54_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ9831861.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            5978..8680
                     /locus_tag="IYF54_001526"
     CDS             5978..8680
                     /locus_tag="IYF54_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9831862.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8832..9149
                     /locus_tag="IYF54_001527"
     CDS             8832..9149
                     /locus_tag="IYF54_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9831863.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9179..9499
                     /locus_tag="IYF54_001528"
     CDS             9179..9499
                     /locus_tag="IYF54_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9831864.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9607..11067
                     /locus_tag="IYF54_001529"
     CDS             9607..11067
                     /locus_tag="IYF54_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9831865.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11135..11356
                     /locus_tag="IYF54_001530"
     CDS             11135..11356
                     /locus_tag="IYF54_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831866.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11387..11569
                     /locus_tag="IYF54_001531"
     CDS             11387..11569
                     /locus_tag="IYF54_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ9831867.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11569..11982
                     /locus_tag="IYF54_001532"
     CDS             11569..11982
                     /locus_tag="IYF54_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ9831868.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12105..13286
                     /locus_tag="IYF54_001533"
     CDS             12105..13286
                     /locus_tag="IYF54_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9831869.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            13675..13748
                     /locus_tag="IYF54_001534"
     tRNA            13675..13748
                     /locus_tag="IYF54_001534"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13709..13711,aa:Arg,seq:cct)
     gene            complement(13785..13985)
                     /locus_tag="IYF54_001535"
     CDS             complement(13785..13985)
                     /locus_tag="IYF54_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ9831870.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(14290..15522)
                     /locus_tag="IYF54_001536"
     CDS             complement(14290..15522)
                     /locus_tag="IYF54_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ9831871.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            15776..16345
                     /locus_tag="IYF54_001537"
     CDS             15776..16345
                     /locus_tag="IYF54_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ9831872.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(16523..16963)
                     /locus_tag="IYF54_001538"
     CDS             complement(16523..16963)
                     /locus_tag="IYF54_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ9831873.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            17121..17885
                     /gene="map"
                     /locus_tag="IYF54_001539"
     CDS             17121..17885
                     /gene="map"
                     /locus_tag="IYF54_001539"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ9831874.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            17917..18840
                     /locus_tag="IYF54_001540"
     CDS             17917..18840
                     /locus_tag="IYF54_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ9831875.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            18916..20055
                     /locus_tag="IYF54_001541"
     CDS             18916..20055
                     /locus_tag="IYF54_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ9831876.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20048..20848
                     /locus_tag="IYF54_001542"
     CDS             20048..20848
                     /locus_tag="IYF54_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9831877.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            20848..21675
                     /locus_tag="IYF54_001543"
     CDS             20848..21675
                     /locus_tag="IYF54_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9831878.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            21653..22387
                     /locus_tag="IYF54_001544"
     CDS             21653..22387
                     /locus_tag="IYF54_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9831879.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            22487..23353
                     /gene="rfbA"
                     /locus_tag="IYF54_001545"
     CDS             22487..23353
                     /gene="rfbA"
                     /locus_tag="IYF54_001545"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ9831880.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            23367..23939
                     /gene="rfbC"
                     /locus_tag="IYF54_001546"
     CDS             23367..23939
                     /gene="rfbC"
                     /locus_tag="IYF54_001546"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ9831881.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            23961..24989
                     /gene="rfbB"
                     /locus_tag="IYF54_001547"
     CDS             23961..24989
                     /gene="rfbB"
                     /locus_tag="IYF54_001547"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ9831882.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            25087..25938
                     /gene="rfbD"
                     /locus_tag="IYF54_001548"
     CDS             25087..25938
                     /gene="rfbD"
                     /locus_tag="IYF54_001548"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ9831883.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            25973..28006
                     /locus_tag="IYF54_001549"
     CDS             25973..28006
                     /locus_tag="IYF54_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831884.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            28050..29330
                     /locus_tag="IYF54_001550"
     CDS             28050..29330
                     /locus_tag="IYF54_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ9831885.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            29540..30346
                     /locus_tag="IYF54_001551"
     CDS             29540..30346
                     /locus_tag="IYF54_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831886.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            30358..31578
                     /locus_tag="IYF54_001552"
     CDS             30358..31578
                     /locus_tag="IYF54_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831887.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            31568..33154
                     /locus_tag="IYF54_001553"
     CDS             31568..33154
                     /locus_tag="IYF54_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9831888.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            33193..35331
                     /locus_tag="IYF54_001554"
     CDS             33193..35331
                     /locus_tag="IYF54_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ9831889.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT        11212 a         6067 c         7356 g        10753 t
ORIGIN      
        1 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
       61 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
      121 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
      181 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
      241 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
      301 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
      361 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
      421 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
      481 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
      541 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
      601 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
      661 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
      721 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
      781 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
      841 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
      901 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
      961 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
     1021 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
     1081 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
     1141 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
     1201 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
     1261 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
     1321 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
     1381 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
     1441 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
     1501 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
     1561 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
     1621 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
     1681 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
     1741 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
     1801 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
     1861 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
     1921 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
     1981 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
     2041 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
     2101 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
     2161 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
     2221 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
     2281 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
     2341 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
     2401 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
     2461 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
     2521 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
     2581 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
     2641 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
     2701 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
     2761 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
     2821 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
     2881 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
     2941 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
     3001 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
     3061 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
     3121 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
     3181 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
     3241 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
     3301 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
     3361 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
     3421 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
     3481 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
     3541 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
     3601 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
     3661 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
     3721 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
     3781 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
     3841 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
     3901 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
     3961 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
     4021 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
     4081 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
     4141 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
     4201 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
     4261 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
     4321 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
     4381 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
     4441 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
     4501 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
     4561 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
     4621 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
     4681 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
     4741 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
     4801 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
     4861 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
     4921 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
     4981 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
     5041 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
     5101 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
     5161 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
     5221 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
     5281 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
     5341 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
     5401 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
     5461 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
     5521 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
     5581 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
     5641 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
     5701 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
     5761 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
     5821 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
     5881 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
     5941 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
     6001 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
     6061 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
     6121 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
     6181 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
     6241 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
     6301 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
     6361 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
     6421 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
     6481 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
     6541 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
     6601 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
     6661 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
     6721 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
     6781 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
     6841 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
     6901 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
     6961 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
     7021 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
     7081 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
     7141 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
     7201 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
     7261 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
     7321 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
     7381 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
     7441 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
     7501 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
     7561 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
     7621 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
     7681 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
     7741 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
     7801 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
     7861 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
     7921 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
     7981 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
     8041 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
     8101 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
     8161 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
     8221 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
     8281 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
     8341 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
     8401 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
     8461 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
     8521 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
     8581 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
     8641 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
     8701 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
     8761 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
     8821 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
     8881 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
     8941 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
     9001 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
     9061 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
     9121 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
     9181 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
     9241 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
     9301 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
     9361 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
     9421 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
     9481 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
     9541 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
     9601 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
     9661 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
     9721 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
     9781 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
     9841 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
     9901 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
     9961 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    10021 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    10081 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    10141 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    10201 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    10261 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    10321 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    10381 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    10441 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    10501 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    10561 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    10621 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    10681 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    10741 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    10801 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    10861 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    10921 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    10981 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    11041 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    11101 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    11161 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    11221 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    11281 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    11341 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    11401 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    11461 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    11521 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    11581 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    11641 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    11701 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    11761 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    11821 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    11881 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    11941 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    12001 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    12061 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    12121 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    12181 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    12241 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    12301 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    12361 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    12421 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    12481 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    12541 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    12601 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    12661 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    12721 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    12781 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    12841 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    12901 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    12961 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    13021 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    13081 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    13141 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    13201 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    13261 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    13321 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    13381 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    13441 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    13501 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    13561 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    13621 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    13681 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    13741 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
    13801 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
    13861 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
    13921 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
    13981 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
    14041 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
    14101 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
    14161 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
    14221 tctaagccaa tagcttagat cctttattct atgctttata ttattttttg catgtgtgcg
    14281 aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc catcttcacg
    14341 gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg tttgacttct
    14401 attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt tcacactgaa
    14461 ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa
    14521 aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga cttctcctaa
    14581 acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc cttgttcggc
    14641 agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga tgatggtgcc
    14701 agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac tgtcaggtgc
    14761 tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag cattatagct
    14821 tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg taccgggagc
    14881 agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt ttgctaattt
    14941 ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag tcgttttgaa
    15001 aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga agacttttgc
    15061 tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg cttgagttgc
    15121 cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta ctcgatggct
    15181 atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc ttgctccttt
    15241 ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa tacgatccgt
    15301 tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa cgataacctc
    15361 agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg cctgttccac
    15421 gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt ctgcaattaa
    15481 gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa ttcctcctca
    15541 ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa caatgaggct
    15601 ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat gttactactt
    15661 gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac
    15721 aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc
    15781 aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac
    15841 cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca
    15901 accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat
    15961 ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga
    16021 tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta
    16081 cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa
    16141 tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta
    16201 taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca
    16261 tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt
    16321 caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca
    16381 agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc
    16441 cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga
    16501 tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta
    16561 aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg
    16621 atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt
    16681 aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca
    16741 agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag
    16801 ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga
    16861 tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag
    16921 tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt
    16981 ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa
    17041 agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt
    17101 tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg
    17161 cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag
    17221 gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa
    17281 ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg
    17341 aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg
    17401 atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag
    17461 aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta
    17521 ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg
    17581 tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga
    17641 ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag
    17701 aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg
    17761 atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata
    17821 cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt
    17881 attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa
    17941 caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa
    18001 atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt
    18061 attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat
    18121 ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt
    18181 ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca
    18241 agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga
    18301 aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct
    18361 attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca
    18421 atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca
    18481 ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg
    18541 aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca
    18601 caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata
    18661 ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc
    18721 ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga
    18781 gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag
    18841 aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg actcttaatt
    18901 aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt
    18961 ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc
    19021 gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct
    19081 atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg
    19141 tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat
    19201 aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt
    19261 tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa
    19321 ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc
    19381 aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg
    19441 ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt
    19501 ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt
    19561 tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa
    19621 gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg
    19681 gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca
    19741 gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct
    19801 gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat
    19861 actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta
    19921 tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt
    19981 ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt
    20041 catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag
    20101 tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt
    20161 aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta
    20221 taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg
    20281 acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat
    20341 tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg
    20401 tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga
    20461 gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa
    20521 gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta
    20581 tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta aagagataaa
    20641 ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga
    20701 aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa
    20761 gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt
    20821 caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt
    20881 tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa
    20941 aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt
    21001 gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat
    21061 aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat
    21121 acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt
    21181 gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat
    21241 cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc
    21301 atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt
    21361 aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg
    21421 gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat
    21481 gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat
    21541 gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa
    21601 cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag
    21661 aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata
    21721 tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat
    21781 ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga
    21841 taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc
    21901 gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga
    21961 gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt
    22021 ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt
    22081 tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg
    22141 tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat
    22201 gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt
    22261 aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg
    22321 gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa
    22381 acgttgataa aagatgttta aatccttaat atttccatga caaatataac aattgatgag
    22441 ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat
    22501 tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt
    22561 gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg
    22621 aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt
    22681 tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg
    22741 attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt
    22801 attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc
    22861 aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt
    22921 agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa
    22981 atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa
    23041 aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt
    23101 ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg
    23161 aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa
    23221 cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca
    23281 attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt
    23341 ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca
    23401 aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa
    23461 aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat
    23521 cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa
    23581 aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta
    23641 gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc
    23701 ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt
    23761 ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt gatgatccag
    23821 atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc
    23881 gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag
    23941 aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg
    24001 gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg
    24061 ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag
    24121 aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg
    24181 acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc
    24241 catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg
    24301 atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag
    24361 acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc
    24421 cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc
    24481 gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc
    24541 atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat
    24601 tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag
    24661 tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg
    24721 aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg
    24781 catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa
    24841 aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg
    24901 acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg
    24961 aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa
    25021 aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat
    25081 aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta
    25141 ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat
    25201 gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca
    25261 gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat
    25321 gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat
    25381 atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac
    25441 caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa
    25501 gagattttag aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac
    25561 aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat
    25621 gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc
    25681 gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat
    25741 gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa
    25801 gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa
    25861 gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc
    25921 aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa
    25981 acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt
    26041 ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat
    26101 cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag
    26161 agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga
    26221 atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa
    26281 cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga
    26341 tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg
    26401 actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact
    26461 gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt
    26521 ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt
    26581 gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac
    26641 gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc
    26701 ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta
    26761 tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt
    26821 tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt
    26881 gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt
    26941 tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc
    27001 tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt
    27061 atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct
    27121 gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct
    27181 gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc
    27241 gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat
    27301 tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa
    27361 ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt
    27421 tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc
    27481 tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc
    27541 aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga
    27601 attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc
    27661 agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg
    27721 gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc
    27781 agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt
    27841 gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct
    27901 atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga
    27961 tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga
    28021 ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat
    28081 ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt
    28141 ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta
    28201 tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt
    28261 ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa
    28321 gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa
    28381 ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct
    28441 tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca
    28501 tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt
    28561 ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg
    28621 ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat
    28681 ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt
    28741 tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc
    28801 cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata
    28861 tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga
    28921 aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta
    28981 ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta
    29041 ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct
    29101 tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc
    29161 cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg
    29221 aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg
    29281 aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa
    29341 atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca
    29401 tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat
    29461 aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa
    29521 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca
    29581 gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
    29641 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
    29701 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
    29761 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
    29821 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
    29881 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
    29941 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
    30001 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
    30061 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
    30121 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
    30181 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
    30241 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
    30301 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
    30361 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
    30421 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
    30481 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
    30541 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
    30601 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
    30661 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
    30721 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt
    30781 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
    30841 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
    30901 tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta
    30961 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
    31021 aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
    31081 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
    31141 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg
    31201 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
    31261 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
    31321 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
    31381 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
    31441 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
    31501 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
    31561 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
    31621 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt
    31681 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga
    31741 attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt
    31801 tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt
    31861 agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga
    31921 aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt
    31981 caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta
    32041 tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga
    32101 ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga
    32161 agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc
    32221 attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt
    32281 gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt
    32341 caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg
    32401 aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg
    32461 agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
    32521 gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac
    32581 tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga
    32641 aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga
    32701 aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac
    32761 cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg
    32821 gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga
    32881 cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa
    32941 ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc
    33001 aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc
    33061 aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc
    33121 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
    33181 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
    33241 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
    33301 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
    33361 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta
    33421 tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc
    33481 atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa
    33541 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
    33601 gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
    33661 agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac
    33721 cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
    33781 tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat
    33841 gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga
    33901 attaaagtcg ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg
    33961 gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt
    34021 gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt
    34081 gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg
    34141 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcggacaagc
    34201 attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg
    34261 gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg
    34321 tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc
    34381 tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc
    34441 gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac
    34501 ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat
    34561 tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
    34621 acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt
    34681 atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
    34741 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
    34801 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
    34861 ttattttatc cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt
    34921 gcaggacatg cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc
    34981 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
    35041 aatgctgtca atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc
    35101 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat
    35161 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg
    35221 gaaatcaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
    35281 gataatctaa ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa
    35341 ctgtattttc atttttatta atgaaaatac agttctttta atttaatt
//
LOCUS       DACIWG010000031        34654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.031, whole genome shotgun sequence.
ACCESSION   DACIWG010000031 DACIWG010000000
VERSION     DACIWG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.031"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            609..1298
                     /locus_tag="IYF54_001555"
     CDS             609..1298
                     /locus_tag="IYF54_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831890.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1285..2493
                     /locus_tag="IYF54_001556"
     CDS             1285..2493
                     /locus_tag="IYF54_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9831891.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2787..4058
                     /gene="serS"
                     /locus_tag="IYF54_001557"
     CDS             2787..4058
                     /gene="serS"
                     /locus_tag="IYF54_001557"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ9831892.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4607..6091)
                     /gene="guaB"
                     /locus_tag="IYF54_001558"
     CDS             complement(4607..6091)
                     /gene="guaB"
                     /locus_tag="IYF54_001558"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ9831893.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6596..7291)
                     /locus_tag="IYF54_001559"
     CDS             complement(6596..7291)
                     /locus_tag="IYF54_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ9831894.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7381..8481)
                     /gene="ychF"
                     /locus_tag="IYF54_001560"
     CDS             complement(7381..8481)
                     /gene="ychF"
                     /locus_tag="IYF54_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ9831895.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8490..8681)
                     /locus_tag="IYF54_001561"
     CDS             complement(8490..8681)
                     /locus_tag="IYF54_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ9831896.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8702..9592)
                     /locus_tag="IYF54_001562"
     CDS             complement(8702..9592)
                     /locus_tag="IYF54_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ9831897.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9579..10346)
                     /locus_tag="IYF54_001563"
     CDS             complement(9579..10346)
                     /locus_tag="IYF54_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9831898.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10358..11071)
                     /gene="rsmG"
                     /locus_tag="IYF54_001564"
     CDS             complement(10358..11071)
                     /gene="rsmG"
                     /locus_tag="IYF54_001564"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ9831899.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11303..13204)
                     /gene="mnmG"
                     /locus_tag="IYF54_001565"
     CDS             complement(11303..13204)
                     /gene="mnmG"
                     /locus_tag="IYF54_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ9831900.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13221..14618)
                     /gene="mnmE"
                     /locus_tag="IYF54_001566"
     CDS             complement(13221..14618)
                     /gene="mnmE"
                     /locus_tag="IYF54_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ9831901.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14962..15300
                     /locus_tag="IYF54_001567"
     CDS             14962..15300
                     /locus_tag="IYF54_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831902.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15468..16226)
                     /locus_tag="IYF54_001568"
     CDS             complement(15468..16226)
                     /locus_tag="IYF54_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ9831903.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16244..17062)
                     /locus_tag="IYF54_001569"
     CDS             complement(16244..17062)
                     /locus_tag="IYF54_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ9831904.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17059..17415)
                     /gene="rnpA"
                     /locus_tag="IYF54_001570"
     CDS             complement(17059..17415)
                     /gene="rnpA"
                     /locus_tag="IYF54_001570"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ9831905.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17533..17667)
                     /gene="rpmH"
                     /locus_tag="IYF54_001571"
     CDS             complement(17533..17667)
                     /gene="rpmH"
                     /locus_tag="IYF54_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ9831906.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18190..19524
                     /gene="dnaA"
                     /locus_tag="IYF54_001572"
     CDS             18190..19524
                     /gene="dnaA"
                     /locus_tag="IYF54_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ9831907.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19722..20852
                     /gene="dnaN"
                     /locus_tag="IYF54_001573"
     CDS             19722..20852
                     /gene="dnaN"
                     /locus_tag="IYF54_001573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ9831908.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21081..21323
                     /gene="yaaA"
                     /locus_tag="IYF54_001574"
     CDS             21081..21323
                     /gene="yaaA"
                     /locus_tag="IYF54_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ9831909.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21310..22434
                     /gene="recF"
                     /locus_tag="IYF54_001575"
     CDS             21310..22434
                     /gene="recF"
                     /locus_tag="IYF54_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ9831910.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22431..24377
                     /gene="gyrB"
                     /locus_tag="IYF54_001576"
     CDS             22431..24377
                     /gene="gyrB"
                     /locus_tag="IYF54_001576"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ9831911.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24397..26868
                     /gene="gyrA"
                     /locus_tag="IYF54_001577"
     CDS             24397..26868
                     /gene="gyrA"
                     /locus_tag="IYF54_001577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ9831912.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27104..27403
                     /locus_tag="IYF54_001578"
     CDS             27104..27403
                     /locus_tag="IYF54_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ9831913.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27453..27965
                     /gene="ssb"
                     /locus_tag="IYF54_001579"
     CDS             27453..27965
                     /gene="ssb"
                     /locus_tag="IYF54_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9831914.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27991..28227
                     /gene="rpsR"
                     /locus_tag="IYF54_001580"
     CDS             27991..28227
                     /gene="rpsR"
                     /locus_tag="IYF54_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ9831915.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28370..30343
                     /locus_tag="IYF54_001581"
     CDS             28370..30343
                     /locus_tag="IYF54_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ9831916.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFTAIKENMNQIYDKE"
     gene            30349..30801
                     /gene="rplI"
                     /locus_tag="IYF54_001582"
     CDS             30349..30801
                     /gene="rplI"
                     /locus_tag="IYF54_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ9831917.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31075..32442
                     /gene="dnaB"
                     /locus_tag="IYF54_001583"
     CDS             31075..32442
                     /gene="dnaB"
                     /locus_tag="IYF54_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ9831918.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33222..34514
                     /locus_tag="IYF54_001584"
     CDS             33222..34514
                     /locus_tag="IYF54_001584"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ9831919.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10560 a         6738 c         6846 g        10510 t
ORIGIN      
        1 caaaggtcta cttcatcagg agtgtttccc gattcgtttt tggctgccgt agcaactttt
       61 atatcatatc agagagacaa gtaattgtca actctttttt aaaagttttt tcgatttcgt
      121 ttttcgaaat cttccagcaa ctcatttatc ataacttatc gtttgttatt tgtcaagaac
      181 ttttttgaag tttttttcga tagaatcaag cttcgcaagt ggttcttttt taacgactca
      241 gttatcttat catgtcattt ccaacatgtc aacaacttat tttttattat ttaataagtt
      301 attggtgttc ttttgaacaa ataataatat atcaacatca catgtgaaag tcaataagaa
      361 aatgcttatt tatcaacatt tctagcttac atgatcattg gttagttctg gtcctttttc
      421 gttcgtcttt ataactccga gagacgtaaa atccttttgt tttcagttta atgttcatgt
      481 tcttgtctta ttcacaccaa ctagatgtct caccttctaa aattttcctt aacaaagtgc
      541 tgcttttatc tatttctgct tttttttgct agaataatag taacaatatg actctggagg
      601 atttatttat gaaaattttg attgctgacg atgataaaga aatcgttgaa ttattaagta
      661 tctatgttca caatgagggg tatgaagcag taaaggctta tgatgggaaa gaagcattat
      721 ctaaaatcag aacaatccct gatattgaat tacttatttt agatatcatg atgcctgaga
      781 tggatgggat gcaagtagtg aaagagctac gaaaagaatc ccaaatacca atcattatgc
      841 tgacagcaaa aactactgat atggataaga tcaaaggact ggtcgctggt gcagatgatt
      901 atgtgaccaa gccatttaat ccgttagaag taatggctag agtaaagtct ttattacggc
      961 gtacaaaaat gcagattgcc ccagatcagc ctgatgtatt agaagtaaat gcattaatca
     1021 tcaataaaga ctctcatgaa gttaaaacaa tcgatgggaa agaaatccag ttaacagctt
     1081 tggaattcgg tatcctctac ttgcttgcta gtcatcctaa tcgagtattt agcgcggatg
     1141 aaatttttga gcgtgtttgg aaacaagaaa gtattgtctc tgctaaaact gtcatggttc
     1201 acgttagtca tttaagagat aagattgaag aagcaactgg tggtgaaaaa gtcattcaaa
     1261 ccgtttgggg agtgggctat aaaattgacg caagatagta aacgagcaac tgaaaaacgc
     1321 cgtcggatca atttgacttc aaaagaaatc agcgaattgt tagcggaagg aatcatcacg
     1381 attatccttt tgcttctatt aaatgtttct attctggttg ttatcagttc ggtgatcaac
     1441 agttcacctt ctttgacgaa tgcgatttgg gattcaaaga atatttttgc agaacgattg
     1501 aacactgatt tgttctggaa cggccggaat tttattattc cctttttctt tctattagat
     1561 attggtgttt tgtattggcg cttgattcga agatatcgcc aaatgcaatt gcggcatatc
     1621 atcagcgaac ttcactacat cgcaaacgga aattatgatc atcgtatccc ttttgaactg
     1681 agtggtgact taagccgagt ggtaacaagt atcaatggat tggtggatag taccgtagca
     1741 gcgattgaag acgaacgtaa aatcgaaaaa tcaaaagatg aattgatcac gaatgtcagt
     1801 catgatatcc gtaccccact cacgtccatc attggatacc taggactgat agaagatggc
     1861 cagtatcact cagaagaaga tcttttaaag tatacgcata ctgcttatat caaagcaaag
     1921 cagatgaaat ctttagtgga cgacttattt gaatacacta aggtacgcca gcctgctgtc
     1981 ccggtgaatt tttctgcctt tgacatgatc cagttgatcg aacagttagc agctgatttt
     2041 gagttggaag cttctaaaaa aaatatccaa atacttgttc aatcaaaagt agactccctt
     2101 atcatggatg gcgataccga aaaacttgta cgtgtcttca ataatctttt gacgaatgcg
     2161 ttgaaatacg gaaaaggagc aacgaagatc gtaatcgaag tagaacgtat cggttcagaa
     2221 gttgttgcaa cagtcaaaaa taacggagca atgatcccac aacaggcaat cgataatcta
     2281 ttcgaccgtt tttatcgtgt agaagaatct cgttcacaag caactggagg aactggactt
     2341 ggtttagcaa tcgctcaaag cattgtggct ttgcacggcg gatacattta tgcaaaatca
     2401 gataagcaat ggacttcttt catcatgcac cttccaatca agaaaaatga aaaattacct
     2461 attgatacac aggaagttat tgacgaaagc tgaaatattc gctattctta ttacaattat
     2521 tagaataaat gaaaaacaca gaacccagta cactttattc tttttcagag agctgatggc
     2581 aggtgtaaat cagcaaagga taatttggaa gttccagttc gtttacgtgc cctttcgaaa
     2641 taagaaaggt cggttcaaca gccgttatgt gttcaacaag tgcagctgac gctgaacttg
     2701 ggtggtaccg cgaatttcag actgattcgt cccaagaata atgggatgga tcagtctttt
     2761 tttattttca aaaaggagga aatcatatgt tagatgtaaa aatgatgcga caaaatattg
     2821 aagaagtaca agaaaaattg atgactcggg gcgtaaaaaa agaaacatta gctgaattca
     2881 tggaactaga tgaaaatcgt cgtaagctac tagtcaaaag tgaagaatta aaaaaatatc
     2941 gaaatgatgt ttctgctgag atcgcccaat tgaagcgtgc aaaagaagat gcttctgcta
     3001 aaatcgctga aatgaaagaa gtcggtggca acatcaaagc attggatact gaaattgctg
     3061 aagtggatga aaaattgaaa caaattgcaa ctactttgcc aaaccttccc catgattctg
     3121 tgccagttgg aacagatgaa gaggacaatg tagaaatccg tcgttggagc gaaccacgta
     3181 catttgcttt tgaaccaaaa ccacactggg aaattgcaga aaatcttgat attttagatt
     3241 ttgaacgtgg cgcaaaagta gccggtagcc gttttgtcta ttacaaagga ttaggtgctc
     3301 gattagaacg tgctgtctac aacttcatgc ttgatcagca tatttacgaa cacggctata
     3361 ctgaaatgat cactccttat atcgtcaaca gtaaagcgat gtttggtacg ggacaatttc
     3421 caaaattcaa agaagatgtt ttccaattgg agaatactga tctaacatta atcccaacag
     3481 ctgaagtacc attaactaac tattataatg atgaaatttt agatggtgca gatttaccta
     3541 tttatttcac tgcactaagc cctgcttttc gttctgaagc cggaagtgct ggtcgggata
     3601 cacgaggact gattcgtctt caccaattca ataaagtaga aatggtcaaa ttttcggatg
     3661 tagagcattc ttacgaagaa ttggaaaaaa tgacagctga tgcagaagat atcttgaaaa
     3721 aattgcattt gccttatcgt gtgatggcct tatcaactgg agacatgggc ttttctgcag
     3781 ctaaaactta tgacttagaa gtatggatac ctgcacaaga tacttatcgt gaaatcagtt
     3841 cttgttctaa ctgtgaagat ttccaagcac gccgcgcaat gatccgttat cgcgataatg
     3901 atggaaaaat ccactatact catactctga atggttcagg acttgcagtt ggccgtacag
     3961 tagctgccat tttggaaaac taccaaaatg aagatggttc tgtcacagtt ccagaagcgc
     4021 tcgttcctta tatggggaat ctgaaagtta ttaaataaag aggctgtgaa aaagccgctt
     4081 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg cataaattgg
     4141 acttaatacc gagaaactgg cttttgaaca ccatttatcg agaggttgtg aaatcagcga
     4201 attaacctga gtattagaga aaaaaatgga aaaatagtcc ctcatatttt accgtttgtt
     4261 gaactaatac cgagggtcag ctgattgaac accgtttagt ggagaggtcg taagcctacc
     4321 ccccagctcc tagttacaag gacaaattca aaaaacagct cttcatattt tatgaatgtt
     4381 gtacttgtga ttcggaggtg gcaagggaac actgtttatt caaaaagagg ctgggacaga
     4441 agcgtttaac tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
     4501 atttcttctt atttccgaag gagttgcttc tacttccgcc gtttatacgt gtttagagcg
     4561 tgagacaaaa gtgttcttta cttttgtccc acgctcttta atgtttttaa gattcaactg
     4621 aatagtttgg tgcttctttt gtgatttgta catcatgagg gtgtgattct tttaatccac
     4681 tgccgctcat ttggataaat tgcgcttcat cacgtaattg ctgtagattg gctgccccaa
     4741 cataacccat tcctgaacgc aaaccgccga tcatttggaa aataatatct gcaacacttc
     4801 ctttataagc aacccgacct tcgattcctt ctggtactaa tttatttgct tcattgacac
     4861 taccttggaa gtaacggtct tttgaacctt tttccatcgc gcctaatgag cccatacctc
     4921 ggtatgtttt gaatcgacgt ccttggaaga tttcgaattc acctggtgat tcatctgtcc
     4981 ctgccaacat acttcctagc atcactgcat gtccgccggc agctaatgct ttgacgatgt
     5041 caccagaata tttgatccct ccgtctgcaa taatcgcttt gccatattga cgagcaactg
     5101 atgctgcatc ataaatagct gtgagctgag gtacacctac accggctacc acacgtgtcg
     5161 tacaaatcga tcctggtccg ataccaactt ttacgacatc tacacctgca tcatataaag
     5221 cttttgtcgc ttcagccgtc gcaacgtttc ctgcaatcaa tgttgcatct gcaaaagttg
     5281 aacggatttc ttgaattttt cggatcacac ctgcactatg tccatgagct gtatcgatga
     5341 tgattgcatc tgcccctgct tctaataaag cattcgcacg ctcaaatgta tcgcttgtta
     5401 caccaacggc tgctgctaca agcaatcgac catgttcatc ttttgctgca tttggaaatt
     5461 cgatcacttt ctcgatgtct ttgatcgtga tcaatccgct taaacgacct tcgttatcta
     5521 cgattggtaa tttttcgatt ttatgttttt gcaaaatctt ttctgcatct tttagagagg
     5581 tacctactgg ggcagtcact aagttgtctt ttgtcatcac atcagcaatc ggcatgtgat
     5641 aatctgttac aaaacgcatg tcgcggtttg taattatccc taccaatttt ctgttttcaa
     5701 gtgtttcaac gattggcaca ccactgatac gatatttgct catcaattct tctgcgtctg
     5761 ctacaagatg ctgtggcgtt aagaagaacg gatcaatgat tacgccactt tcagaacgtt
     5821 tgacttttct tacttcatcc gcttgctggc tgatcgtcat gtttttatgg atcacaccta
     5881 gtccgccttg tcttgccata gcaatggcca tcttgctgtc ggtaacagta tccatacttg
     5941 cactgatgat tggaatattc aacttaatat ttttagctaa ttgtacgctc atatccactt
     6001 cgtttggtaa cacgtgactt tctgctggta tcaataagac atcatcaaaa gtaaatcctt
     6061 ttttcgtgaa ctttgtttcc cagttggaca tttttagtac cactcctcta atttagttta
     6121 tctattaaaa taattgattt tttcatttaa gtcaatgatc tacttggaat caagttagct
     6181 ttctcataga ataccaatat aaagcgttta catctgtgta tattaaaaga gattgtaaaa
     6241 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga
     6301 attggttggg tttattaccg cagatgcaaa cttttgaaca ccgtttagtg gagaggctgt
     6361 gaaaaagctg cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttccacattt
     6421 ttgcataaat tggacttaat accgagaaac tggcttttga acaccgttta tcgtaaaaag
     6481 ggactgtgac aagacttttg tcacagtccc ttactattat ttaactatgg ataaacaaaa
     6541 tgaggttctt tactacccct agatatctag caaaatagct gatctttttg ctagtttatc
     6601 ttcttgggac ggataaactt ccaataatcc cgtatttttt cttcaagtag taacggattg
     6661 ctaaagcagt tgccccgata atgataacta ctactggatc taatactgga ttgataactg
     6721 gtggcaatag tgttgtcgca gaaaacagta cgacccaaag aagtgtcgtt ccacctagga
     6781 taagcaatgt cttacccatt cctggtttct tgcttcgatc tgctccggga tattcatatt
     6841 gatagatgta tttgtacatc atataaaaga cccagccacc gattgcagaa cctagaacga
     6901 gtgtagaaaa gccataagct ggcgttgttc cacgtgaaaa caatccggta atcgcaatca
     6961 tgatacttaa tagaccaagc aacagcaacg tattatctaa ccacatcaat aaaggggtgg
     7021 atgaattttt tacttctgct ggcttattga tgattgcttc cgttctttca gagactgtcc
     7081 caaacaattg acgggctgtt cttccgcctt tttgttctgc aactaaagta ggcagaatgt
     7141 catgaagggc ttttactttt tcttcttcag acaaattcgc tgcttctaaa gattttttca
     7201 aatcaaaaat gtactgttga tttcttttgg ttaattttgt ttctaaacta ttgttttctt
     7261 ttacgattgc tcgtaattgt tctggttcca ttatctaata gtcctcctta gccgttcctt
     7321 ttctcaacgg tcgtcacaaa agacattcta ccatattcta cagctagttt gatagccctt
     7381 ttacacatta aagcggaata acattacgtc gccgtcttgt acgacataat cttttccttc
     7441 taatcgaaca cgtccagctt cttttgctgc atgcatgttt ccgtaatgat ccaagtcttc
     7501 aaaagagaca gtttctgcac ggataaatcc gcgttcgaaa tctgaatgga tgattcctgc
     7561 tgcttgtggt gctttgattc cttttttgaa tgtccaagca cgtacttctt gttccccagc
     7621 agtaaagtaa gtagctaagc ctaacaaatc ataagcagca cggatcaact gatccaaacc
     7681 tgattcttcg attcctaaag cttctaaaaa ttccgctttg tcttcttcat ctaattctgc
     7741 gatttcttct tctgcacgag cactgaccac gatcacttct gcattttctt ctgcagcaaa
     7801 gttgcgcact tcttgtacat aatgattcgc gtctgcatct gctacgtcat cttctgagac
     7861 attggctaca taaagaattg gttttgtcgt caataaaaat agtgatttaa cgattttttg
     7921 ttcttcttca gtaaattcga tcgttcgggc agattttcct tcttccagta caggtttgat
     7981 tttatccagt acagctagtt ctgccactgc atctttatct ttggttttag caacttttgc
     8041 tacacgagta tagcgtttgt tgatagattc taagtctgct aacactagtt ccaagttgat
     8101 cgtatcaata tcttctagtg gatcaacacg tccttctacg tgagtaatat tatcatcatc
     8161 aaaacagcga acaacgtgac aaatagcgtc tacttggcgg atatggctta agaattgatt
     8221 tcccaaacct tctcctttac ttgcgccttt tacgattcca gcaatatctg taaattcaaa
     8281 agtcgtcgga acagtttttt tcggatgtac aagttctgtt aagcgttgca aacgccagtc
     8341 aggtacttct accatcccta cgttggggtc gatcgtcgca aagggatagt ttgctgcttc
     8401 tgctcctgct tttgtgatcg cattaaacaa agttgatttt cctacgttgg gcaacccaac
     8461 aattccagca gtcaatgcca taaatttatt cactctttct ctgcattttt ttcaattact
     8521 ttttttaatt tcttttcaaa atctcgacgg gtcatcatga cgatatgtcc gcaattcata
     8581 catctgattt tgatatctgc gcccattcga atgacttccc agcgattcgt ctggcaagcg
     8641 tgaggttttt tcatttcaac gatatcgtgc aagtcgtaca taaaaatagc ctcctttttg
     8701 tttattcttc atcaaattga atattcaaga tatcaagaat tcgagtcaaa tctttttgag
     8761 aaagatattc aatctcgatc ttacctttcc catttttttc ttggatagct acacttgtac
     8821 caaatttatc catcaaacga tcttcgcttt ctcggatata atatggtttt tcctttgcta
     8881 gccttgggat ttttttcttt tcttttccat cattttgatt taactcagaa acaagctgtt
     8941 ccagttggcg gacagttaaa ttttcagtta ctgcacgatt ggctaatttc aagatcaatg
     9001 ttttattttt taaacctaac agcgtacggg cttgtcccat agataaacgc tgatcttgta
     9061 ccatttcctt gacaagatcc ggtaaagaaa gcaagcgcaa ataattggcg atataaggtc
     9121 tgctctttcc taaacgacta gctacttctg cttgtgttag tttcaggttt ttcatcagca
     9181 tctcatatgc ttctgcttct tctaacggat tcagatcttc tcgctgcaag ttttccagca
     9241 ctgctacttg catcatcgct tcttcgtcaa agtctctaac gatagcaggg atcgtttctt
     9301 tttcagctag tttggacgct cgaaaacgtc gctcccctgc aataatctca tatcctttta
     9361 ctgcggaacg gcgtacgatg atgggttgaa agacacctga ttgttcaata gaattagcta
     9421 gttcctgcaa agatacttct tcaaatgttt tacggggctg atacggattt ggtcgcagct
     9481 cattcaatgg tagctggaca acggtatcat cttttacatc caccgtctct aagttttcta
     9541 aatcttgaaa cagtgcatcg attcctcggc ctaatccttt acttttcttc acgacttacc
     9601 acttcctttg ccaatgcttg gtagacttca gctcctcgag aacgtggatc gtagtcgatg
     9661 atcggttttc catgactagg tgcttctgat aaacgaatat ttcttggaat gatcgtttcg
     9721 taaacttttt cgcggaagta tttacgtact tcttctacga cttcatttcc cagatttgtt
     9781 cgtgcatcat acattgtcag taagactccc tcgatctcta attctggatt aaagtgtttt
     9841 tggactaaac gaaccgtatt cagtaattgg cttaatcctt ccaatgcgta atactcacac
     9901 tgaacaggta tcaaaatcga atcacttgct gtaaatgaat tgattgtcag atggccaaga
     9961 gaaggcgggc aatcgatcaa aatataatca tactgactgc ttacttctgc taaagatcct
    10021 tttaatcttg attctcttgc catcattgac gtcaattcga tctctgctcc tgctaattgg
    10081 agagtcgcag gcacaatact caaattttcg tgttcagtga tcaaagttgc ctcatcaatt
    10141 ggcaattcat tgactagaac atcatagatg tctcttgtca catcaggttt tctgatcccg
    10201 acaccactgg tagcatttcc ttgtgcatcc atatccacta gcaatacttt tttgccaagg
    10261 ctagcaagac aagcacctag attcaccgtc gtcgttgttt tgccgacgcc acctttttgg
    10321 ttcgcaacag atattatttg tgccatctct tctcctccta tttgattggc tgcttattag
    10381 gcagtccggg ctttcttggg tatttctttg gggttggttt ctttttttga ataacgatga
    10441 tatgccgttg atcagagaca ttcggcaagg taaccgcctc ttctttgagg aatttaccac
    10501 ctaataaagc aatcgcttgt tttgcttctg tcaattcctc ttcacttttt gctgctttca
    10561 atgcataaaa atagccttct tgtttagcca agggcaaaca caattccgct aacacattca
    10621 aacgagcaac cgcacgagca gtgacaaggt caaatgatgc acgaaagtga ggattttgcc
    10681 caaatgtttc tgcccggtca tgatataagg aagcatcgat tcctaataat cgggttaact
    10741 cagataagaa ggttattcgt ttattcagag aatcaacgat cgttatctgc agttctggaa
    10801 aggcaatttt taaaggtatg ctaggaaacc ctgcccccga accaacatcg cataaagata
    10861 aaggttgcgt aaagtctact tcaaatgcta atgtaagtga atcgtaaaaa tgcttcagat
    10921 atacgtcttt ttcatccgta atagctgtaa ggttcatctt ttcgttccat tcgactaatg
    10981 tccgataata aatagcgaat tgattcattt gatgatcggt aagctctatc cctttttctg
    11041 ccaaggattg gcgaaattct tctggtatca taaatattcc tttcattgtg aaacagcaat
    11101 tgtttcacag attctctccc tactttaacg caaaatctct agacatgcaa tccttccata
    11161 tggattctta aaaatttgaa aagtaagaaa agccgataga ctttttcttt ctagtatgac
    11221 tcataaaata tacacagaag agagaactag ctttgagcaa tttctgcaaa aactagttct
    11281 ctcttctatt tattattacg tattacgctg gttcactgac tttcgcaatc ttcccttgtt
    11341 cgatatagac cattaggata cttaggtctg cagggtttac gccactgatt cggctagctt
    11401 gggcaattgt ttctggttgg attttctgca atttttgttt tgcttctgtt gctaatccgt
    11461 tgattgctgc gtagtcgatg ttttctggaa tctttttcgc ttccatgcgt tttaattttt
    11521 ctactttttc catggctttt ttgatgtagc cttcatattt gatttggatt tctacttgtt
    11581 cgatttcttt tcgtgtcaat tcttcatttt ctggaataaa ccgtagaagt tctgcataag
    11641 taatctcagg acgtcttaaa aagtcgcttg ctagtacacc atcttttaat ggtgccgcat
    11701 taatcgttgc taaaaagtct tggacttctt ctgttggttt gattcgtaca ttcatcaaac
    11761 gtttgatttc ttgttcaact gcttcttttt tcttcaaata attcttgtat tgatcttctt
    11821 ttactaagcc tacacggtga cccatttctg ttaatcgcaa atctgcatta tcatgacgta
    11881 aaagcagacg gtattcagca cgtgaagtca gcaaacgata aggttcgttc gttccttttg
    11941 ttaccaaatc atcgatcatg acaccgatgt aaccgtcact tcgtttgatc actaaaggtt
    12001 cttttccctg aactttcaaa gctgcattga taccggcaat cagcccttgt ccagccgctt
    12061 cttcataacc gcttgtacca tttgtttgtc cagctgtata aagattttca acgactttag
    12121 tttctaatgt cggacggagc tggtgtggct ctactacgtc atattcgata gcatatcctg
    12181 tacgcatcat ttctactttt tctaatccag caatagaatg aagcatgttg acttgaacat
    12241 cctctggtaa agaagtagat aaaccttgaa catagacttc ttccgtattt aagccttctg
    12301 gttccaaaaa taattgatga cgtggtttgt ctgcaaaacg cacgatctta tcttcgatgg
    12361 atggacaata gcgagcgcct accccttcaa cgatccctgt aaacattggg gcacgatgta
    12421 agttgtctcg aataatttgg tgagtcgtct cacctgtata ggtcagccag caagattcct
    12481 gatctagttt ataggcacta tctggtgtgc tgaagctaaa atgattcggc tctttgtcac
    12541 caggctgctc ttctgtaaca gaatagtcga tcgtacttga ttttactcga ggcggtgttc
    12601 ctgttttgaa ccgttcgata gcaaagccaa gttcttttag atgattcgct agtccaatag
    12661 aaggttgaga gttattcgga cctgatgaat atttcaattc accgataatg atttctccgc
    12721 gaagtgctgt tccggctgta ataacaactg ctttggcccg gtagctcgct cctgtggacg
    12781 tcactactcc atgacaaaca ccatcttcga cgatcagttc ttcgacgatt ccttgtctta
    12841 gcgtcaaatt tggttctttt tcgatcgtat gtttcatttc tgttgaataa gcatgtttgt
    12901 ctgcttgtgc tcgcaatgct cggacagctg ggccttttcc tgtgttcaac atacgcattt
    12961 ggatataggt tttatcgata ttcttaccca tttctccgcc taacgcatcg atttctcgaa
    13021 cgacaacgcc tttagctggt ccgcctacag aaggattaca tggcatgaat gctaccatgt
    13081 ccaaatttat tgtcagtaat aatgtttttg atcccatacg tgcagcagca agtgcagctt
    13141 ctgaaccggc gtgtcctgcg ccaacaacga tcacgtcata cgcatctgct tcataatgtt
    13201 ccaatcgttt gttcctcctt ttattttcct aaacaaaatt gactgaataa ttgagtgatt
    13261 agctcatctt gtacactgtc gccgacaact tctcctagat agtcccagca acgcgtcata
    13321 tctatttgaa cgagatccac aggcattcct gaatcaatac ctgcaatgac ttcttcaagt
    13381 gagagggaag ctttttctaa caacgcgata tggcgggtat tagaaacata agtagcgtct
    13441 ctttctcctg tttctccgct gaagaataaa tcagaaatag ctgattccaa acgatctagt
    13501 ccgtcgtttt tagctacgga aattgaaaaa actggttcat tttcgatcaa tttcttcaat
    13561 ttttcttgtt ccagctgtgc tggcaaatct gttttattta gaagaatgat ccgtttcaat
    13621 ccacttgtcg cttccaataa ttgttcatct tctgctgtca gtggttcgct ttggtttaga
    13681 acaagtaaga tcaagtctgc ttctgctaat gcttttcggc ttctttcgac cccgatccgt
    13741 tccaccacat cttctgtttc ccgaattcca gctgtatcga ttagcttgag cggtaccccc
    13801 cgaacattga catactcttc gattacatct cgagtcgttc ctgcaatatc cgtaacgatt
    13861 gctttttctt ctcttaacaa atggtttaac aaacttgatt tgccgacatt tggccggccg
    13921 atgattgccg tacttaaccc ttcccgcaga accttccctt gcttggatgt agcaagtagc
    13981 gcttggattc tttgttggat catctgcgct ttttcaagca ataacttcgt agtcaattct
    14041 tcgacatcat catattctgg ataatcaata tttacttcta cttgtgctaa tgtctctaaa
    14101 atctcttgtc tcaatgagcg gatcaaagca gacagattgc cgtctaactg attaagagct
    14161 agtcccattg ctttgtctgt ttttgcccga atcaaatcca tcacagcttc tgcctgtgaa
    14221 aggtcaacac gaccattcaa gaatgctcgt tttgtgaatt cgcctggctc agctaatctt
    14281 gcaccttctc ttaacaatag ctgcaaaatt tgattgacta caacgatccc accgtgacaa
    14341 ttgatttcta ctacatcttc ccgcgtaaat gtctttggtg ctctcatcac ggagaccatg
    14401 acttcatcga caagctgatt gctcttggga tcaacgatat gtccataatg gatcgtatga
    14461 cttggtactt ctgataatcg tttgtttcca gattgataaa ctttatctgc tagttgcaca
    14521 gcttgatcgc cacttaaacg aacgatactg attgcacctt ctccaggtgg ggtagaaatt
    14581 gccgcaatcg tatcaaattc taaagtaatt tcagccattt tttcacttcc tttttcattt
    14641 tgcgcacaaa aaaagtgccc actccaccct ttatgcacaa taaacggtga acttcgcact
    14701 ttgactgctt gttcttgatt aaattctggt catagattat cacacttgct ataaggaatc
    14761 aagtgtttaa aacaattaga tgaagttgag cctgttctgc ttccattacc ttatatttaa
    14821 ttgtccttgg atgtctcttt gcgttctttg gtttattcga agagaagata ctgtataaac
    14881 aaggctttat ctttcttaaa aaaaacggta cactaaatgg tagcgctatc taaactatac
    14941 acttttgaaa ggagaaaata tgtgagcccg ttcagatctg ctgcattgga agaagaaagt
    15001 gaccctcgga tcaacgatgt cgttttatct acagggaata tcaaccagaa atatatcgtc
    15061 cgcgatatcg tttttgttgt ggaatgctta gaagctgatt tgtttgaccc atcttttccc
    15121 aaagatgacc ttttcttaac aacgaagagg aaattaaaga cgaaggtact tgaatatggt
    15181 gcgaatgcag taatcaactg tcattttgag tatcagcaaa aaaaacgcga cggaaagtat
    15241 tttctggaac tttttgctta tggaacagtg atccaattta tccaaacaac gattggttaa
    15301 atgaaacaga caaaaaaagc ggatcaaaac tgaagacagt ttcgatccgc ttttttctat
    15361 caaagttagt gactacttca tcgtatactc tgataagaat gatttctagt aaaatatact
    15421 tggtttgaaa aactttgtca acgtgattta ttcaaaaagt aatggaatca ataatatttt
    15481 ttagcaggtt ctactactaa ataacgataa ggttcctcac cttccgaatg tgtttttaca
    15541 tgttcatctt tgcttaatgc agcatggatt tgtttacgtt caaatgcagg cattggttct
    15601 aagaaaacag gctgccctgt ccgttttact ttttcagccg tgcgatcagc taaacgctga
    15661 atgatctctt ggcgcttttc acggtaattg cctacattga ctactacaga cagcttattt
    15721 cttgctaaac gatggatgaa gacttgtgcc agatattgca atgcattcag tgtttttcca
    15781 tgtttcccaa tcagtatgcc ttgcttttgt gtttctaaat ggaaaacaac catattattt
    15841 gattctcttt ccattttgac aagagctgga gcatcaagct gttttgaaat ttcagtaaga
    15901 taaactgcca attccgttaa agctgcttca tcacctagct cacttacttc ttctgttttt
    15961 tcttcaagaa tcgtttcgtt gactacctcg atctcagctg ttggttcttc ttccaaaatt
    16021 tcttctacag tctgttctac tgtttcagaa actttttcgc tgatacttgg ctggatcgaa
    16081 agccgtgcat tctttcgacc cattcctagg aaaccttttt ttccttcttc cagtatttcg
    16141 atttcgactt ggtctttggc caaacccaaa tcagccagac ctagttggac agcttcgtct
    16201 attgttgcag cttcataaat cggcattagc attacctccc ctattatttc cgttttttct
    16261 taggattcat tgctttctta agagcacgtt ctctttcgcg gattctccgt gcttcttctt
    16321 cacgctcttt tcgaattttg aaagggttgt tgatcagcag cgtttgtaca acttggaagg
    16381 cattggaaac tacccagtat aatgaaagac cgctggctaa attgacaccc atcactaaaa
    16441 tcattacagg catcacgaaa ttcatgatct tcattgatgc attcgattca atttgactca
    16501 tgctagataa atacgtactt gcaaaagtaa agattgctgc taaaatcggc aaaataaatg
    16561 ttggatccgc gttccctaag ttcaaccata agaaatgccc ttgtttcaac tctggtacac
    16621 gtgaaataga ttgccataac gccatcaaga ttggcatttg tactaataaa ggaagacaac
    16681 cagcatatgg attgacgcca ttttctgcat ataaacgctg ctgctcttct cttaacttac
    16741 tttgtgtttc cggatctctt gaactatatt gttgctgcag tgcttttaat ttcggttgta
    16801 attcttgcgt tttacgcatg ctctttgttt ggaaatgcat caacggcaat aggatcacgc
    16861 ggatgatcag tgtaaataaa atgatcccta tccctgcatt accaaatgac aacgccttga
    16921 tcgcctcagc aaagtaataa acaatataac gatcccaaat tcctgtactc tgtgcgctga
    16981 cttccccagt tccgcaaccg gataagacga tgaccaaact aacgataccg gccatcaaaa
    17041 gtaaacgttt gtatttcttc acttactctt ttcctctctg tcgattattt ttgcaagttt
    17101 taagacgtgg actaaattgg agcgaacctc atcataagtc aaccccttca cacttggtct
    17161 agcaatcaca ataaaatcca actccggatc aatttcttct tttatagaat aaactgctgc
    17221 tcggattttt cgtttgactt cattgcggtt taccgcgttg ccgacctttt ttcctacaga
    17281 taaaccaact cgaaaatgag gctgtcccgg tttttccaaa cggtaaacga cgaatttgcg
    17341 gttagcaaaa gaatctcccg tatggaacac ggcttgaaac tctcgttctt tttttactcg
    17401 atacgctttt ctcattagtt ttctttcctt ttctatctct cacttacgct cataatcata
    17461 ccataattac gattgtcctt tcatcatttc cctaaaaaag agtataaaaa aaaccactga
    17521 aaccccagtg gcctaagctg aaagaacttt tcttcctttg cgacgacggc tagctaacac
    17581 acgacgtcca tttttagtac tcatacgttt acggaatccg tgaacttttt gacgtttacg
    17641 tttatttggt tggtatgttc ttttcatata ttccacctcc aaaatgttct tatctatttt
    17701 ataaacacag acatactttg atagtataac gacaaagtag tgattttgtc aatataaaat
    17761 agaagatcga ttgttaaaac ttatccacaa gattattttt ttctatggga atcctatgtg
    17821 gaaaaagtta tgcacaggga ttatccacag ataataaaag gctggtttta cacagaaaac
    17881 acacgtgtaa aaaaagtaat ccacaatcag gcgatttcct gtgtattccg tctcctaact
    17941 attggtatat ctgtattttt tatccacaga cgcaagagga aatctattgg tttttacgag
    18001 ttttccacct gtgcattagt ggtgtggaaa actttttttg acaactgtgt ttttctcttt
    18061 tttctttttg tcgattgtgt ggataattgt aattaaaaaa tttttttctt cggtttctgt
    18121 ggaaaactat tgaaaaaaat gctaaattag tactagttat tcacttttta atgaaggagg
    18181 aggccttata tggtatccct cgatgcttta tggaatgaat taaaagcaac ataccaaaag
    18241 gatttatcgc ctgctagcta taatacatgg atcgaaacag cgaaccctcg aacccttgat
    18301 caaaaccagc tcgttgttga ggttcctagc aagattcata aagaatattg ggaaaaaaac
    18361 ctggcgacaa aaatcgttga agtcggttat atgttatcag gaaatgagat tatccctcgc
    18421 tttattactg gcgaagaagc agagcaagaa gaagttatag aagaaaaaaa tccaaaagtt
    18481 gtggcaccta gtccgctaaa aaaagccatg ctgaacccta aatatacctt tgatacgttt
    18541 gtcattggta aaggaaacca gatggctcat gcagcagcgc tcgttgtagc agaagatcca
    18601 ggttctattt ataacccgct cttcttttat ggaggcgtgg gacttggtaa gactcacttg
    18661 atgcatgcaa ttggtcatca aatgctgcaa agtcagccta atgccaaagt aaaatatgta
    18721 agcagtgaaa cttttgctaa tgatttcatc aattcgatcc aaaacaaaac agcagaagag
    18781 ttccgtcaag aatatcgaaa tgttgattta ctgctagtgg atgacattca attttttgca
    18841 gaaaaagaag ctacgcaaga agagtttttc catactttca atgccttgta caatgaaggg
    18901 aaacaaatcg tactaaccag tgatcggttg ccaaacgaga ttccgaaact gcaagaacgc
    18961 cttgtttctc gctttgcatg ggggctgtct gttgatatta caccgccgga tcttgaaaca
    19021 agaacagcga ttttacgtaa aaaagcggcg gctgaacgtc tagagatccc agatgacaca
    19081 ctaagctaca tcgcaggaca aattgattct aacattcgtg aattagaagg tgcgcttgtt
    19141 cgagtgcaag cttttgctgc gatgaatagt gaagatattt cgactagtct agctgcagac
    19201 gcactgaaaa cgttaaaatc aggaaaagga catcctcaat tgtcaatcct gcagatccaa
    19261 gaagaagtgg caaagtatta tcacatccaa ttgaaagatc tcaaaggaaa gaaacgcgtc
    19321 aaatctattg ttgttcctag acaaatcgct atgtatttgg cacgtgagct cacagataat
    19381 tctttaccga aaatcggagc tgaattcggt ggcaaggatc atacaacggt tatccatgct
    19441 catgaaaaaa tccagcagct gatggattct agtgtcagta tgcaaaatga agtatcagaa
    19501 atcaaaaact ttttactaaa ttaatttgtc gattctatca actgaaaata gcatgtggat
    19561 aaaagaaaaa aacgttcaaa agttatacac actttttcca cagctgtttt ttcgtttctc
    19621 tcttacgttt tttgagtttt ccacagaatc aacatgccct attactttta ttattctttt
    19681 taactaataa taaataaaca aacattcaaa ggagatacat catgaaagtt actttaaacc
    19741 gagctagctt tatgcaggaa ttgcaaactg ttcaacgagc tatttcaagc aaaaccacga
    19801 tccctatttt gacaggtgta aaaatcacac tgacacaaga aggtttgact ttgacgggga
    19861 gcaacgctga tatatcaatt gaaacttttt tgtctgttga aaacgaaaaa gcaaatatgc
    19921 aaatcgaatc tactggttcc attgttttac aagcacgttt ctttagcgaa atcattcgga
    19981 gacttcctga agaaacattt actttagaag ttttagaaaa taaacaagta gcgatcactt
    20041 ctggaaaagc gaattttatc gtaaatggat tagatgcaga taactatcct catcttcctg
    20101 ttgtcgaaag ccataaccag atgaaattac ctgtacacgt attgactaaa ctaatcaacg
    20161 aaacagtttt tgctgtctca caacatgaga gtcgtccaat cttgacaggt gtgcatttca
    20221 ttttatctga taattcttta ttagctgtag ctactgattc tcaccgtcta agtcaacgcg
    20281 tgattccagt agaacaagcg gctgatcatt ttgatattgt tattcctgga aaaagtttga
    20341 tcgaattatc tcgctcatta acaaatgaag aagaaatcgt cgaaatcagc attatggaaa
    20401 accaagtgtt gttcaaaaca gaaacgatgt atttctattc ccgtttgcta gaaggaaact
    20461 atccagatac caatcgtttg attccttcaa gttttaatac agaggttgaa ttttctgttc
    20521 ctagcttttt agcggcgatc gaacgggctt ctttactttc tcatgaaggc cgtaacaaca
    20581 tcgttcgttt atctattcgt ccagatgcgg ttgttttata cggaaattcg ccagaaatcg
    20641 ggaaagtaga agaaagcttg agttatacag caagcagtgg cgatccatta gatatttctt
    20701 tcaatccgga ttatatgaaa gcagcgttgc gtgcatttgg agatatgagc atcaaagtga
    20761 aatttatctc tgctattcgt ccatttacat tggaaccaac ggaagatggc gttcaattta
    20821 ttcagctgat tactcctgta cgtacaaatt aacaatgatt ttcaatgttc ttgaaaattt
    20881 aattgaaaag aaatagaaaa aataaaattt taaacagaag gccctaaaat ctcttttaag
    20941 gctttctgtt tatttttgtt taaatcacct tttttagatt aaatcgtctt atatggaaaa
    21001 atatatggat aaatttgttt tattgcgaaa aaaaaggtat aatagagtat acttttaata
    21061 atgaaaatga gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt
    21121 tgggccaaat gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt
    21181 tggcagaaaa cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt
    21241 atcccggcat gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa
    21301 acgacgataa tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg
    21361 aacatcgaat tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg
    21421 aatcttttgg aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag
    21481 caggaattga ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt
    21541 tcatccactc ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac
    21601 catatcgaac agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca
    21661 ccagaagatc tttctttagt aaaaggcagc ccgcagacca ggcggaagtt tttagatatg
    21721 gaattaggcc agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg
    21781 aaacaaagaa accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat
    21841 ttggatattt tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca
    21901 cagttcgtca agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa
    21961 aaagagcagc tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa
    22021 gagcaaatcc aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt
    22081 cggggaacaa cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa
    22141 aatgtgcaga cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta
    22201 gcagaaatcg atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat
    22261 gtgatgagcg agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa
    22321 gttcaaacgt tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa
    22381 cctgagattt tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag
    22441 aaagaagttt agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag
    22501 gacttgaagc cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc
    22561 tgcatcacct tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg
    22621 caacgaaaat ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc
    22681 gggggatacc tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta
    22741 ctgttttaca tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc
    22801 acggagtagg atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca
    22861 aagaaggaaa tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga
    22921 ttatcgaaca aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt
    22981 ttacagagac aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt
    23041 tcttgaaccg cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca
    23101 atgaatacca ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa
    23161 ccgtgatttt tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag
    23221 tctctatgca atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc
    23281 atacgtatga aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa
    23341 atgattatgc ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag
    23401 acgtgcgaga aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag
    23461 gacaaacaaa aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta
    23521 gcgaacattt catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa
    23581 aaggattact agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac
    23641 gtaaaggtgc tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg
    23701 atcctgaaaa atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc
    23761 aaggtcgtaa tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg
    23821 aaaaagcaag catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta
    23881 tgggtacagg gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta
    23941 tcatgactga tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt
    24001 atcgttacat gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat
    24061 acggggtaaa acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag
    24121 agttgaaaca agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata
    24181 aaggacttgg agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc
    24241 ggatgatggc ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt
    24301 tgatggggga tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga
    24361 agaacttaga tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac
    24421 atccatgatg tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt
    24481 gttattgtcg cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt
    24541 attttatacg gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc
    24601 agaatcgtcg gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag
    24661 tcaatggtac ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga
    24721 aactttggtt ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg
    24781 agcaaaatcg ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc
    24841 aactatgatg atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta
    24901 gtgaacggaa caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg
    24961 tctgaggtag tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa
    25021 ttgatggaag tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca
    25081 ggaatccgaa gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag
    25141 attactgaga tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc
    25201 aataaagcaa aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg
    25261 atcacagatt tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt
    25321 cgtgacgcca gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat
    25381 tcatttggat tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa
    25441 caaattcttg aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt
    25501 gaaaaagaaa aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat
    25561 catatcgatg aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg
    25621 acgatgatcg aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt
    25681 ttacgccgat tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta
    25741 aaactgattg aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc
    25801 aagaatgaat tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta
    25861 gtcggtgagg tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt
    25921 acattgacaa acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga
    25981 aggggtggac gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg
    26041 gtttcttgtt ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg
    26101 gcaaaaggat acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat
    26161 ttactaggta tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca
    26221 gaagaaggcc actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc
    26281 actgcttttg ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat
    26341 gagctcgtca atgtgctact gacagatggt caatctaata tcattattgg aacacataac
    26401 ggctactctg tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg
    26461 gttagaggaa tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa
    26521 gatcaagaag tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa
    26581 tatccagtga aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat
    26641 ggtccactag ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat
    26701 aaaggtgtca ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta
    26761 ggcgtacgtt tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa
    26821 ccagaagctg cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta
    26881 attgtgtaag ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt
    26941 gattagttga atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa
    27001 aatagagtgt ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc
    27061 ttaggaacct aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata
    27121 tgaaatcatg tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga
    27181 acgtttcgat gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga
    27241 aaaacgccgt ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa
    27301 agtatcttct ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa
    27361 cgacgatatc attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa
    27421 acaatagaaa acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac
    27481 gtctgacgaa agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta
    27541 ctttggctgt gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca
    27601 actgtgtgat ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac
    27661 ttttaggtgt tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg
    27721 tatacgtgac agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg
    27781 aaaatcgcca acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg
    27841 gaaacaataa ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg
    27901 atacttctga cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat
    27961 tctaaacgaa tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa
    28021 cgtcgtaaag tagactacat tgctgctaac catattgaat atatcgatta taaagatatc
    28081 gaattactaa aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt
    28141 acaggcgcta aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc
    28201 ttgctacctt tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg
    28261 atcagctatt caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa
    28321 tgacgaaagt tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa
    28381 aaacaatcat ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt
    28441 ggtagtctta attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt
    28501 atggggtgtt cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa
    28561 aatccagcag gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc
    28621 tgtaggagtg attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc
    28681 attgaaaatc aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac
    28741 tgcaaaagaa aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat
    28801 ggatacagta aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact
    28861 ccatcagacg aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc
    28921 cattgataaa ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt
    28981 ttctgactgg atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt
    29041 cttcgtagca cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga
    29101 tcgctttcga gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc
    29161 ttatggctca agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc
    29221 tttagtccgt ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt
    29281 ttattatggg gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat
    29341 gagtaccgct ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa
    29401 gtttccggat atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa
    29461 ccagaaaaaa gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg
    29521 tcttgaagaa atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc
    29581 catagagctg aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct
    29641 atcgatctct cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg
    29701 tcgtggggaa gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc
    29761 agcttcagag ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga
    29821 tcgttttgag gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt
    29881 ccgtacaact gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc
    29941 atccttagta caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt
    30001 gattgctgga agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa
    30061 agagtataat agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat
    30121 caatgcagct ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag
    30181 taccggcacg atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac
    30241 aaacgcagct actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttac
    30301 tgcaatcaaa gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt
    30361 tttttacaag atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt
    30421 tatgcacaaa attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc
    30481 agtcagttag caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa
    30541 gctaaaaagt taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa
    30601 gcaggggaag acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta
    30661 aacaaacaat acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt
    30721 tcactaggct acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa
    30781 gttcatgtcg tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca
    30841 gaaggaaaaa agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa
    30901 gctgcaaatc gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa
    30961 agaaaacgag tataataaaa gagaactatt tttttattac actttcggta cgaatgatga
    31021 cttttctctc attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat
    31081 gaagtgtggc aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc
    31141 tctgtttttc ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac
    31201 ttttatcgtc gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat
    31261 gaagcgattg acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat
    31321 gccggtggtt taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc
    31381 gtctactatg cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca
    31441 acaggaatcg ttacgaaagg gttcgagcaa ggagaagaag ttcaaacaat tttagatgat
    31501 gccgaacgca gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt
    31561 gctgaagtac tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa
    31621 atcacaggtc taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc
    31681 gaagaactaa tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat
    31741 attgctcaaa atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg
    31801 ggggcagaat ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat
    31861 ttacgtacag gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt
    31921 ctttctcgag caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt
    31981 gcgaaatgtc ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat
    32041 ttgcagttga ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct
    32101 cgccaactga aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta
    32161 tctcgtggag tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc
    32221 ggatcaattg aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa
    32281 agagaaggcg gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc
    32341 atcatcgaga agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa
    32401 tacaataagt tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct
    32461 tgagcaatcc cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag
    32521 tacttaattt aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt
    32581 tccgaataaa cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata
    32641 tgaggaacta ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac
    32701 ctcttttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca
    32761 aacatgagaa gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca
    32821 caacctcttt cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac
    32881 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
    32941 ttcacaacct ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt
    33001 tcacaaaaat ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact
    33061 tctgttacaa cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt
    33121 ttttataaaa taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat
    33181 acaatggttc aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag
    33241 taggtacgca atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg
    33301 ctgaagtgat tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg
    33361 gtgtgactta taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg
    33421 tgatcggaaa cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac
    33481 atgaaaaagg tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc
    33541 cttatcatat caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta
    33601 caacgattaa aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta
    33661 ttgcagactt attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca
    33721 aaaaccgtca atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg
    33781 aagaatatta tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct
    33841 tgaatgatgc tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc
    33901 tggatatcga ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg
    33961 taacaatcgg aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag
    34021 cctatacgtc acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag
    34081 aacaaatccg cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag
    34141 gctggtttga ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat
    34201 ctttaaactc gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg
    34261 aattagatgg cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca
    34321 aaccaatcta tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg
    34381 cagaactgcc agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac
    34441 gtatctcaac attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct
    34501 gggcgcaaat ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc
    34561 gaagtgttta tctatacgat aataataact tgaacactgt ttattcagaa taagggactg
    34621 tgacaaaaac cgtgtcacag tccctttttc cgaa
//
LOCUS       DACIWG010000032        34601 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.032, whole genome shotgun sequence.
ACCESSION   DACIWG010000032 DACIWG010000000
VERSION     DACIWG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.032"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            214..498
                     /locus_tag="IYF54_001585"
     CDS             214..498
                     /locus_tag="IYF54_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ9831920.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IYF54_001586"
     CDS             complement(779..1198)
                     /locus_tag="IYF54_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ9831921.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IYF54_001587"
     CDS             complement(1200..1784)
                     /locus_tag="IYF54_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ9831922.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IYF54_001588"
     CDS             complement(1831..3306)
                     /locus_tag="IYF54_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ9831923.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IYF54_001589"
     CDS             3487..3798
                     /locus_tag="IYF54_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831924.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IYF54_001590"
     CDS             complement(3913..6249)
                     /locus_tag="IYF54_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9831925.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IYF54_001591"
     CDS             6391..7059
                     /locus_tag="IYF54_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831926.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IYF54_001592"
     CDS             7062..8105
                     /locus_tag="IYF54_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9831927.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IYF54_001593"
     CDS             complement(8342..9223)
                     /locus_tag="IYF54_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9831928.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IYF54_001594"
     CDS             9382..10110
                     /locus_tag="IYF54_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ9831929.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IYF54_001595"
     CDS             10266..10583
                     /locus_tag="IYF54_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9831930.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IYF54_001596"
     CDS             10576..11184
                     /locus_tag="IYF54_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ9831931.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IYF54_001597"
     CDS             11200..12030
                     /locus_tag="IYF54_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ9831932.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IYF54_001598"
     CDS             12168..12656
                     /locus_tag="IYF54_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ9831933.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IYF54_001599"
     CDS             12707..13579
                     /locus_tag="IYF54_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ9831934.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IYF54_001600"
     CDS             13755..14117
                     /locus_tag="IYF54_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9831935.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IYF54_001601"
     CDS             14126..14878
                     /locus_tag="IYF54_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831936.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IYF54_001602"
     CDS             14892..16694
                     /locus_tag="IYF54_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831937.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IYF54_001603"
     CDS             16971..18011
                     /locus_tag="IYF54_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ9831938.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="IYF54_001604"
     CDS             complement(18063..20207)
                     /locus_tag="IYF54_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9831939.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="IYF54_001605"
     CDS             complement(20564..21937)
                     /locus_tag="IYF54_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9831940.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IYF54_001606"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="IYF54_001606"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ9831941.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IYF54_001607"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="IYF54_001607"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ9831942.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="IYF54_001608"
     CDS             complement(23385..24398)
                     /locus_tag="IYF54_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9831943.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="IYF54_001609"
     CDS             complement(24479..25078)
                     /locus_tag="IYF54_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9831944.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="IYF54_001610"
     CDS             25264..25965
                     /locus_tag="IYF54_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831945.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="IYF54_001611"
     CDS             25975..28686
                     /locus_tag="IYF54_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ9831946.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="IYF54_001612"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="IYF54_001612"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ9831947.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="IYF54_001613"
     CDS             complement(30825..32006)
                     /locus_tag="IYF54_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9831948.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="IYF54_001614"
     CDS             32258..33781
                     /locus_tag="IYF54_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ9831949.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="IYF54_001615"
     CDS             complement(33850..34578)
                     /locus_tag="IYF54_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9831950.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT        10935 a         6190 c         7021 g        10455 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaatctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatatacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt t
//
LOCUS       DACIWG010000033        31372 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.033, whole genome shotgun sequence.
ACCESSION   DACIWG010000033 DACIWG010000000
VERSION     DACIWG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.033"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            271..1140
                     /locus_tag="IYF54_001616"
     CDS             271..1140
                     /locus_tag="IYF54_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ9831951.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="IYF54_001617"
     CDS             1349..2374
                     /locus_tag="IYF54_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ9831952.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="IYF54_001618"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="IYF54_001618"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ9831953.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="IYF54_001619"
     CDS             3457..5160
                     /locus_tag="IYF54_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9831954.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="IYF54_001620"
     CDS             5162..7195
                     /locus_tag="IYF54_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ9831955.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="IYF54_001621"
     CDS             7206..7646
                     /locus_tag="IYF54_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9831956.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="IYF54_001622"
     CDS             complement(7846..8973)
                     /locus_tag="IYF54_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9831957.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="IYF54_001623"
     CDS             complement(8976..9653)
                     /locus_tag="IYF54_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9831958.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="IYF54_001624"
     CDS             9917..11683
                     /locus_tag="IYF54_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ9831959.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="IYF54_001625"
     CDS             11698..12135
                     /locus_tag="IYF54_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9831960.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="IYF54_001626"
     CDS             12148..13299
                     /locus_tag="IYF54_001626"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ9831961.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="IYF54_001627"
     CDS             complement(13361..14059)
                     /locus_tag="IYF54_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ9831962.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="IYF54_001628"
     CDS             complement(14052..15404)
                     /locus_tag="IYF54_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ9831963.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="IYF54_001629"
     CDS             complement(15627..17027)
                     /locus_tag="IYF54_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ9831964.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="IYF54_001630"
     CDS             17188..17925
                     /locus_tag="IYF54_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ9831965.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="IYF54_001631"
     CDS             18050..18529
                     /locus_tag="IYF54_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ9831966.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="IYF54_001632"
     CDS             18526..19293
                     /locus_tag="IYF54_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831967.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="IYF54_001633"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="IYF54_001633"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9831968.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="IYF54_001634"
     CDS             complement(20196..20798)
                     /locus_tag="IYF54_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9831969.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21373
                     /locus_tag="IYF54_001635"
                     /pseudo
     CDS             20904..21373
                     /locus_tag="IYF54_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21479..22063
                     /locus_tag="IYF54_001636"
     CDS             21479..22063
                     /locus_tag="IYF54_001636"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ9831970.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="IYF54_001637"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="IYF54_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ9831971.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="IYF54_001638"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="IYF54_001638"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ9831972.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="IYF54_001639"
     CDS             23964..24143
                     /locus_tag="IYF54_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831973.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="IYF54_001640"
     CDS             24165..25190
                     /locus_tag="IYF54_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ9831974.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /locus_tag="IYF54_001641"
     CDS             25365..26609
                     /locus_tag="IYF54_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ9831975.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="IYF54_001642"
     CDS             26762..27709
                     /locus_tag="IYF54_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9831976.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="IYF54_001643"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="IYF54_001643"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ9831977.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="IYF54_001644"
     CDS             complement(28611..28853)
                     /locus_tag="IYF54_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831978.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="IYF54_001645"
     tRNA            28974..29046
                     /locus_tag="IYF54_001645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
     gene            complement(29165..29803)
                     /locus_tag="IYF54_001646"
     CDS             complement(29165..29803)
                     /locus_tag="IYF54_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9831979.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29926..30558)
                     /locus_tag="IYF54_001647"
     CDS             complement(29926..30558)
                     /locus_tag="IYF54_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ9831980.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30909..31208
                     /locus_tag="IYF54_001648"
     CDS             30909..31208
                     /locus_tag="IYF54_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9831981.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10699 a         5479 c         6314 g         8880 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgtt gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29161 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29221 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29281 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29341 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29401 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29461 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29521 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29581 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29641 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29701 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29761 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29821 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29881 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29941 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    30001 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30061 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30121 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30181 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30241 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30301 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30361 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30421 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30481 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30541 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30601 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30661 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30721 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30781 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30841 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30901 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30961 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    31021 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31081 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31141 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31201 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31261 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31321 tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt at
//
LOCUS       DACIWG010000034        31230 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.034, whole genome shotgun sequence.
ACCESSION   DACIWG010000034 DACIWG010000000
VERSION     DACIWG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.034"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(400..717)
                     /locus_tag="IYF54_001649"
     CDS             complement(400..717)
                     /locus_tag="IYF54_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9831982.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYEMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="IYF54_001650"
     CDS             complement(718..972)
                     /locus_tag="IYF54_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9831983.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1232..1450)
                     /locus_tag="IYF54_001651"
                     /pseudo
     CDS             complement(1232..1450)
                     /locus_tag="IYF54_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3263..4303
                     /locus_tag="IYF54_001652"
     CDS             3263..4303
                     /locus_tag="IYF54_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ9831984.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SSPSTITKDWLE"
     gene            5144..5476
                     /locus_tag="IYF54_001653"
     CDS             5144..5476
                     /locus_tag="IYF54_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ9831985.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            6095..6436
                     /locus_tag="IYF54_001654"
     CDS             6095..6436
                     /locus_tag="IYF54_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ9831986.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6455..6745
                     /locus_tag="IYF54_001655"
     CDS             6455..6745
                     /locus_tag="IYF54_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831987.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6937..7170
                     /locus_tag="IYF54_001656"
     CDS             6937..7170
                     /locus_tag="IYF54_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831988.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7576..8247
                     /locus_tag="IYF54_001657"
     CDS             7576..8247
                     /locus_tag="IYF54_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ9831989.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8300..10279
                     /locus_tag="IYF54_001658"
     CDS             8300..10279
                     /locus_tag="IYF54_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ9831990.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDT
                     AGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASL
                     HGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLK
                     VDSGLFGTGIKATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKS
                     DKEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKAS
                     VPMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            10294..11046
                     /locus_tag="IYF54_001659"
     CDS             10294..11046
                     /locus_tag="IYF54_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9831991.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11062..11823
                     /locus_tag="IYF54_001660"
     CDS             11062..11823
                     /locus_tag="IYF54_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831992.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            11836..12096
                     /locus_tag="IYF54_001661"
     CDS             11836..12096
                     /locus_tag="IYF54_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831993.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12093..14183
                     /locus_tag="IYF54_001662"
     CDS             12093..14183
                     /locus_tag="IYF54_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ9831994.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            14210..14674
                     /locus_tag="IYF54_001663"
     CDS             14210..14674
                     /locus_tag="IYF54_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831995.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14671..14913
                     /locus_tag="IYF54_001664"
     CDS             14671..14913
                     /locus_tag="IYF54_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831996.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14931..15233
                     /locus_tag="IYF54_001665"
     CDS             14931..15233
                     /locus_tag="IYF54_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831997.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            15303..17366
                     /locus_tag="IYF54_001666"
     CDS             15303..17366
                     /locus_tag="IYF54_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9831998.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            17366..19975
                     /locus_tag="IYF54_001667"
     CDS             17366..19975
                     /locus_tag="IYF54_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ9831999.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            19972..22368
                     /locus_tag="IYF54_001668"
     CDS             19972..22368
                     /locus_tag="IYF54_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832000.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            22420..22752
                     /locus_tag="IYF54_001669"
     CDS             22420..22752
                     /locus_tag="IYF54_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832001.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22753..23346
                     /locus_tag="IYF54_001670"
     CDS             22753..23346
                     /locus_tag="IYF54_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832002.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23509..25203
                     /locus_tag="IYF54_001671"
     CDS             23509..25203
                     /locus_tag="IYF54_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ9832003.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            25891..27768
                     /gene="ltrA"
                     /locus_tag="IYF54_001672"
     CDS             25891..27768
                     /gene="ltrA"
                     /locus_tag="IYF54_001672"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ9832004.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            27899..28276
                     /locus_tag="IYF54_001673"
                     /pseudo
     CDS             27899..28276
                     /locus_tag="IYF54_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            28276..29481
                     /locus_tag="IYF54_001674"
     CDS             28276..29481
                     /locus_tag="IYF54_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ9832005.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            29497..30141
                     /locus_tag="IYF54_001675"
     CDS             29497..30141
                     /locus_tag="IYF54_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832006.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            30152..30958
                     /locus_tag="IYF54_001676"
     CDS             30152..30958
                     /locus_tag="IYF54_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832007.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            30981..31211
                     /locus_tag="IYF54_001677"
     CDS             30981..31211
                     /locus_tag="IYF54_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832008.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
BASE COUNT        11448 a         4816 c         6202 g         8764 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgatg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattt catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgata aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca
     1201 tatgtcggtc ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta
     1261 cacgaataaa gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata
     1321 gcaatactgg aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg
     1381 catacttaaa tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa
     1441 atggatcaat agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac
     1501 gttaaaaaat attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca
     1561 aaactgatcg atattgtatc caaaaatata tactacctaa aataggtagt atatattaaa
     1621 atatgtgctt ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag
     1681 gtattttagt tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg
     1741 gaggaggaga tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag
     1801 aggtaactgc acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta
     1861 agcctacccg ttaattctat aatatagaaa cgtaaaaaaa aaccccatca gcctgcacgc
     1921 tgatggggtt tggtgatata atgaaatacc tctttgttac tcataattat accataatta
     1981 gtcacgaatg caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat
     2041 gcattgtcca ccaaatggat tttgtgaagc attaaataca ttgttattaa ttgtcgctgc
     2101 ttttaactag gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc
     2161 tactacctta aaccaaaatt taagtactac ctaatttaag tagtacttaa ataatctaaa
     2221 aaatagttat cccaattgtt atgattttat aatacgatta tttgtaacaa aatgaatatt
     2281 atataattaa acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt
     2341 tattctattc tcttaacttc aactaagtta tgctgcacat ctttatataa atcatatgca
     2401 actcattagc tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa
     2461 ataaagtagt ttataattaa caagtatata tatttaaaaa tttaaaatta atttttttat
     2521 gacttgtaca aatttatctg atattctctt attaagaact acctaatttg ggtagttctt
     2581 attttttaca ttttaagtag tatttaattg caatttggta gtattttttt attatcttat
     2641 tcctagaacg gtagtactta atataaatat aggtagtatc taatttaagt attaatggta
     2701 gtatctaatt taacaggtag tatgtaacta ctttaaagta atttttttca gattaacaca
     2761 gtagtatctt attaaataag ttaattctaa aggtagtaca taattattat atatatctaa
     2821 cattggtagt acttattgta ttttttagta gtatataatc tattgttttc tagcctaaag
     2881 ccttgtctta taagaataat ataatatata ctactgaaat aaggtagtat atattactag
     2941 tagtggtagt atgttttata tgcataaggt agttcttaat ttattttttg ttttgatact
     3001 ggtagtactt aattatcctt aattttaaaa ccaaatttta ttcatacgtt cttctggtag
     3061 tatttaatta taaataatat ttatttaaag tagttcttaa tgactttaaa ggtagtacat
     3121 aattcatttt atttcttatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg
     3181 cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca
     3241 acgtgaaagt agaggatcta caatgactga ttttaatttt tttaaggcgg atagagttta
     3301 caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat
     3361 gaaagattca actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag
     3421 caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg
     3481 tagcgtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt
     3541 tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa
     3601 tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt
     3661 tgatagagag aatagggaaa atgttgataa atcagagggt atgaaaatca tacccaccct
     3721 cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg
     3781 ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt
     3841 agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt
     3901 agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt
     3961 attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac
     4021 tattcataat gcaaagaaaa aagctgaaat agaaagtggt atttcgatag tttttgaaga
     4081 actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta
     4141 tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa
     4201 aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaattc
     4261 tgaaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt
     4321 gattgttgct tcatggagta acattaaagt taaaaatttt ttattattct aattatatta
     4381 cgaatattac aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa
     4441 aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata
     4501 aaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
     4561 gataatttca ttatttaaac aaactaaaaa tcttgatatt acttcttttc aggcaatacg
     4621 aatattattc gcttacattt ttttgtataa atttagttgt tttaaattta attgtctgct
     4681 atgcctgtga tgaattgatt ttcgttctgc acttttggac gataaatttg gacaattgaa
     4741 aatacaaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
     4801 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
     4861 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
     4921 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
     4981 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
     5041 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     5101 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
     5161 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
     5221 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
     5281 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
     5341 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
     5401 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     5461 gcgtttaatc agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     5521 tgaaaatatt gtcgagactt ctaaaagaac ttagaatcat tagaaaaata aggagtagac
     5581 ttgatctatc aacaatttcc ttcggggagt gacggggtag atttttacga ttggtaacgt
     5641 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     5701 aataatatac acagagtaaa caagagggaa tttagaggtt agacaagtta gaatagcgcc
     5761 tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag
     5821 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     5881 tgtagatact tgcgaggacg ttggtcattg ggtcaatcga gcatttaccg ctgtctacta
     5941 gggtttctcg tttcggacgc cataaacgag tcgatatttt ttatgtattt ttaagtaaac
     6001 gaaaaacgat aaaatatgaa cgagtcctga ataaagtgaa aaaattgata taaaacatag
     6061 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     6121 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gttttgtgac
     6181 gtttagaaga ggcggaaatt gtaaaggatt agatgtttta gcattaaacg aggatgattt
     6241 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     6301 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     6361 agatacttgg tcatatttga gtgattatat tgtcagcgtt gaaatcattg aatttgtagc
     6421 ggataaagaa gaataaaaac tggaggaata aattatgaaa tttttcgaag taaaatcacc
     6481 atactattat gcgttaatcg cagcaaagaa tgaagaagaa tgtgcagaat tgtatgaaca
     6541 agtagtaaca gatatcgaaa atcgtgaaga atttattcga tgtttgcatg agttaaatcg
     6601 aaatgaagca attcaagagg atgcgaaaaa aattagtgaa gaaacgttac aaccaatcgg
     6661 tatggaggaa gctacaaaag acatttttac aattattgat gaacaaaaat cttgtgtatt
     6721 aagcattgat tctacattgg tttaatagat ccaagattct ataatttttt tcaaagtaaa
     6781 aagaaattag gtaatgattc tcagaccaat tacttaggaa aataaccata caaattagat
     6841 tttacagata aaggattaga aggaatcacg gcgattaata aaaaggtagt attaccttag
     6901 gcattgagta aggaatttat ttaggaggaa aattagatgg cgcgaccagt tttagatgcg
     6961 atggatatgg atactttgac aaaaggaagg tatgtacagc tgttagctcg tggttattca
     7021 cctaaagatg tattcaaagc cctatctaaa ggaacagata tggaaaaaga aagattacga
     7081 aaagaattcg actattggag agagcataac ggaataaaag atttaaaacc agcacgtcca
     7141 gttattagac gaaaaaaatt gaagaagtaa gaccaaagcg tatggcgtac ataggctttt
     7201 ttattttgat aaaaataatg aaaggaagga aaactcatga attgtaaaga aatcgaaaat
     7261 agaaaaaaag tgtcgaaaga aatggaagaa aagttactaa aaacaatgaa acaaaaacaa
     7321 tgaaacaaaa acaccttaaa agattatctg tcgcgcaata tatcaatgat atgcagttaa
     7381 caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag ttgagacgaa
     7441 cttatgtgaa aaaaagttct aattgtcaac tattgttaga agtctcatga cattcattaa
     7501 tagaattcat ttttaaaact ttttctatgg aaatgagaaa aagttttttt gttttgtctg
     7561 aaaggaggca gatggatgaa aaaatggtta tttggttttt taggagtcgc tttaatcgtc
     7621 ttttgcagtg tgtttggcta tgttagctac caaaaacatg aaggagaggt gtttaagcaa
     7681 aatatagaaa aaaagatgcc catagatcaa atcaatgaac atgcaaagag ttataaggag
     7741 gatgcaacga acgttaataa tgatatgtct ttgggacaaa tgttgtccat acaaaaagaa
     7801 gcaatcgaaa tgggcgtcaa taaacaagcc tttgcacaaa ttcagattcc tgcgcttggt
     7861 ttaacattgc cgatttttaa aggtgcaaat caatacacat tgagtcttgg tgcggctact
     7921 tatttttatg aggatgccga aatgcgcaaa ggaaactatg ttctagcagg tcataacatg
     7981 gaaattccag gtgttttatt ttctgatatt caaaagttgt cactagggga ggtgatggac
     8041 ttagttagta acgatggcgt ctatcgttac aaggtaacgc gtaaatttat cgtacctgag
     8101 tactttaaat taatcgatgg cgttcctgaa gagaactctt tcttgtcttt accaaagaaa
     8161 ggagagaaac ccttattaac gttgtttact tgtgtttata cgtcacaagg aaaagaaaga
     8221 tacgtggtac agggagagct acaataacaa ggttttaatc aacgtgagtc gttgaaatat
     8281 gaaacgagga gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg gaatgcttgt
     8341 tggaatgtta ggaatcattg caggctgctt tcagggatat cgagcatatg cagaagatgt
     8401 cacgcaaaaa acgccaccag agaaggtcaa tatcacggtt cataaattaa tgtatgatca
     8461 aggaacgcaa ttgaatgttg atgttgacgg gatcaaaaac gatggttata cgcacgatca
     8521 atatcctacg ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt
     8581 aggaaatatt acggatcaag cattaccgag ggaagattct gatctaacca acgcgaaagt
     8641 ggacgaaatc gttaaagatg tagaagacaa gggatctaac agtgagtacg tcaagaatgc
     8701 aaccaacaaa attacttcag ctgtagatga aaatggtgag attacttttg ctgatcaacc
     8761 agcctatgtc aacaattcca aagggaatgt atatgtggtt tatgaaagta aatccgctgc
     8821 tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga ctgataatac
     8881 aggttcaggt tttttaaaag acattcactt atatcctaaa aatattgttt ctaagttgag
     8941 ttttgaatta acgaaatttg gtgacgatgg cacagctcaa agcaaacaaa cacctttaaa
     9001 aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat taggagatct
     9061 tgtatcggat gatcaaggaa agctgactgc tactgatttg acgttaggaa aatactattt
     9121 tgtggaagtt ccttcagaag ttgtagtggg atcagataaa gaaccaacag ccgatcaata
     9181 tttattaggc gcagacgctc gcaacgatgc gcacaataaa ttaacctttg aaattacgaa
     9241 tgatggcgtg acaagtgact taaaagcttc atacgtaaac tataaagcac cagtgatcga
     9301 taaaacagta acaaatggaa caggacaaga acattctttc caaattggtg acgcagtaaa
     9361 ttatcaagga acgattcata tcccaacaga cattgcaggc ggagcagacg gtatcacagt
     9421 aaacggagtg aaaagcgaaa cttctcctta cagtgtattt aaatgggggg atacagcagg
     9481 acaaggactt tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata aagatggatc
     9541 tgtggtatta aaagaaaata cagattataa aatccaaaat tcagaaaatg gctttgtgat
     9601 cgattttatc gtgaataatg gccaagtaag tgatactgta gcaagtttac acggacaaga
     9661 tcttcaaatg acttataaca tgtatgtaaa tgacagcgca gctgttgcca atccattaac
     9721 aaatagcgtg gatttcgttt ataacaacaa tccatttaat caggaagaac accacgaaaa
     9781 aacgaaagct gacgttgtga cttatggtgc aaaattctta aaagtcgata gcggactatt
     9841 tggaacagga atcaaagcaa caccgcttga aagcgcagaa tttgctgcta aaaacgcaga
     9901 aggaaaatat tatggtggat tagttgatac ggataaaaac ggtgtcaaag aagcagtatg
     9961 ggtagacgat gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc actttgaaat
    10021 cactggttta actgaaggtg aatattcact ggaagaaaca aaagcgcctg aaaactatca
    10081 aaaattaacg aaagagatct ctttcaaagt agacaaagat tcatacaaag aagaaaaccg
    10141 aattacgatc aaaaacaatc aaaaagcttc cgtaccaatg accggtagca acggttttca
    10201 aacgtatgta ttaatctctt gcttgttgct aggagcaggg gcactttctg cagttgttta
    10261 ctttaagaag aaagcctaga aggatcaaaa ccaatgatta tcaggaagcc taagaaaaag
    10321 cgaatcatgg gtaaatggat cattgctttc tggttacttt cagctgtagg tgtcttactt
    10381 ttgatgcctg ctgaagcgag cgtggcgaaa taccaacaaa atcagcagat tgccgcaatc
    10441 gatcgaacag gaacggcagc agaaacggat tcttctttgg atgttgctaa gattgagtta
    10501 ggcgatcctg ttggtattct tacaattcca agtatttctt taaaactacc aatttatgac
    10561 ggaacgtccg acaaaatttt agaaaatggt gtaggtatta cggaaggaac tggtgatatt
    10621 acaggaggaa acggtaaaaa tcctttaatc gcaggtcata gtggacttta taaagataat
    10681 ctttttgatg atttaccttc agtaaaaaag ggagaaaagt tttatatcaa agttgatggt
    10741 gagcagcacg cttatcaaat cgatcggatt gaggaagtgc aaaaagacga actacaaaga
    10801 aactttgtca cttacttaga accaaatccc aatgaagatc gagtaacgct aatgacatgt
    10861 acgcctaagg ggatcaacac acaccgtttt ctagtgtatg ggaagcgagt aacctttaca
    10921 aaatcagaat taaaagatga agaaaataag aaacaaaaat tatcttggac atggctttta
    10981 ggaagtactg tttttctttc agtcatgatc attggttcgc tatttgttta taaaaagaaa
    11041 aaataaagga gggtaaccaa catgagaaat agaaaatggc tatttgtttg tgcagccgct
    11101 cttttttctt tcactgccta cacacagttg acgtttgcag acagacaaga aagcacggat
    11161 agcacagaac aaacaatcca aacgtcagat agcagcacga ttcaaagcag cgatgaaacc
    11221 gtcgattcgt caacagttga ttcttcagaa gaaccgaaaa ttgctgaaga cgatcaaata
    11281 gtcaaacaga ccattcacgt tcaaaaaatt atttcttccg aacaattaga caaccagggg
    11341 caaaaacttg aaaaacaaga aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg
    11401 gacattttac aaaagatgga tgttaaggat ctcactactg accaaattga atcccaatta
    11461 aaagatcgag tgaaaaaact attcagtgat caattgaagc tagtttctaa tggaaaaaca
    11521 aagacaattg atcaacaaac aggtgtcttt gaattctcga tagaagtaca ggcaaatcag
    11581 aaacaagctt attatattgt caatgaatca agtcctgaaa acatttctaa tagcgaggat
    11641 attttgctat taacacctgt ttctgataaa aacggtgaat tcttaaagga tgtgtggatc
    11701 tatcctaaat cagaagcttc gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc
    11761 gtaaagaaaa acttctttga gaattgttgg gattttgtta ctcacttgtt ttctagagat
    11821 taaggaagaa ataaaatgaa actgatcaaa ttattattat tcaatttttc ttgtttaacc
    11881 gcagtctttt tgtactgcgg tttattattt tctaaactat ctattttgcc aagctaccaa
    11941 ctatacctaa tgaggatttt aaacatacca gctgcttcca tgccgctaga tattatggaa
    12001 aagctcatta ggaatccatt atttactacg atcaatttga tcgttattgt cggaatttca
    12061 ctagtcattt ataaaaaaga ggggatgaag ggatgaattt taaacactcg tggacaagac
    12121 ggttaaaata tgtttttccc ataatgatga tgttaggtac attgtttggg ttaaaaacag
    12181 ccaatgtctt tgctgaaaaa gttattgtcg atccgtcaaa tccaatccag aacgtaaaga
    12241 ataattttat tattccaaaa gatgttccta atgcaaaaac aaatattact gtaaacggag
    12301 cgtcaaaagt ttacaaatat catgttgatg ccaacaaagg tggttttaca gacgactacg
    12361 taggattagt ggagggaaac aaaaatatac gctatccttc tttccaaaaa gaatacggtg
    12421 aaacaaacct tgttttagaa ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa
    12481 aaatcggtac gtataacggg aaaaaagtca atatcaagct agttctatct aatattcact
    12541 tatactcgga tacgttgccg tggaatctac tagataacaa ctatacaaaa actcattttc
    12601 gagacgatgg ctataaaaat acgaatggtg caatgagtaa atctaaaaaa agaacagtac
    12661 tttggatctc agacaatctt ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc
    12721 agctagtagc cacatatgag gacggtagcc ccgttcaatt ttcaggagat acatttatca
    12781 gttttaactc tttgaaccct gcaggaggaa aaaccacgga tcttaaagga gaatatgccc
    12841 actatgacaa aatgaatacg acagattggt atgtcagaaa agatacggtt ctttcagaat
    12901 ttaaatcttt ctacaacaat ctgaacgtgg taggtggcca cccaggagga agctctaagc
    12961 tgacacaagc ggataatgat ttcaataatt tgcatgataa attaggagat ccgaagtttg
    13021 gtcaaggtac tgtgtccttt aaaatatctg aagcaaatcc gacgtttgtg attggttctt
    13081 ctaatgttca aacctggttt actttatctt ctgcgacaat ctttagtgtt gttcctgatc
    13141 aacctgaaaa aacaggggtt gataaaaatg gaaataacgt gaacgataaa atgttgcaag
    13201 taggagacac gattcaatat cgaatcaaac agaaggtgaa tcgattagga gtggatttac
    13261 tagcggttta cgatcgattt gagttaatcg ataatttacc aaaagaagta aattacgtcg
    13321 atgcacacgt tgaaagtggt acaaataaaa aattcgatgt atctggtgaa gtgacctatg
    13381 acaaaacaaa acaccaggta aaatatgcag cgaaagctga tacattaaaa aatcgaatga
    13441 agtataacgg cgaaacttac gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc
    13501 aaaatagtgt agccaaaaat cagggaacgt cgatcattaa taaagtagaa aaggatacaa
    13561 atatcattac cgtttatttc cctaaaattc cagtgaaaga agtacaacaa aatggaaaag
    13621 atgtaaacgg acgaaatgac ggtaagaagg gtacgccgac agctccttta aacgcaggat
    13681 ctgaagtaca atatctagtc acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag
    13741 atcactacaa acaatttagt attcaagatc caattgaagc tcgtttgaca tataaagaag
    13801 gttctgcgca agtaatagat aagagtaaag gaaaagatat tacttctgaa ggcacactaa
    13861 cctatgatag caattcaaga acgctgaagt gggaagcttc cgctgacttc ttaagtaaca
    13921 atcttttaga tggacgagaa attcagctga tatttacagc taaaactcca ttacaatcag
    13981 aaaaaaatat tgataaccaa gccgtagtag cagtagataa tgtttctaat aaaacaaatg
    14041 ttgtgacaat tggggttgat cctaacttgc cacaggtcat tgttcctaaa acaggttcta
    14101 cacatttagt aacgatttca gcggtcagct tactattact tgtgttagca acttttagtt
    14161 atgcggttct gagatacatt taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt
    14221 aataatagta gttacttcct tgattgccat gattgttatt ttagtcggat gtagtaatga
    14281 aaaaaataaa caaactaatc aagataatgg cgtattgaaa tcaggaacta tgtggaaaga
    14341 agaagtaggt ggtcttgttt ataatttgaa aattatagac gagactacct gggaatactc
    14401 tgagagtgta tggcatccag atcctgtaca aataacagtc aagaggcaaa aagactataa
    14461 aggtttagaa agatacaaaa tagtagattc agctggtgtt agggaattta taaacaaatc
    14521 tgattcttta tttattgttg ttccctatga gaagaatggc gtaaaaaaaa taatttttct
    14581 tggatctagc aaggatgaaa agcgaacgaa agagaaattg attcacgatg gatcacaatc
    14641 caataaatat aagttacaaa agactagcga gtgattgcta gtctttttat ttgtcctgaa
    14701 ggaggaatca cagtggcaaa agctaaaacg ccatttgaaa aagccgcgtc taaaatctta
    14761 gaacgccgcg gtgtatctga aacacagtgg aaacaagaag tccttgaaaa agcacaattt
    14821 gacctattac ggggaaaaga caaggactta gaaggatata tctatcaacg tgaacgtgaa
    14881 aagcttgttt tcgacgaaat tattaaacac taaaaggagc gaacaatttt atgtttgaat
    14941 caattacaat gaatttttta acacaaccga aagtagtagc tgcggttgat atttttagag
    15001 cattacaaac tgccgcaact agtttaaaaa acaatctgat gatttttgct gctgctgcgg
    15061 ccgttgcttc cattgttatt gggggtcttt tcttcctatt tggacgaaga ggagctgaaa
    15121 atgggaagat gcacgtaggc tatgtattga tgggggtagg agttatttta gcagcagctt
    15181 ctattgtggt ttcgttattt ggattgtttg gacaaacacc tcaaggatta taagaaagta
    15241 aaaaagtagg agtaagaagt agtcgatact ttttactcct tttttatgaa aggagaatac
    15301 tcatgtactt tacgattgca ggcaatacca aaacagataa agagttaata aaaaaagcaa
    15361 cgaaggaagt aggaaagcgt acttcttttt taccgaaaac aattaaatat tctgtcattg
    15421 aaacggaaga acagctacat gtgttaggga aagtactgga acacgaaggt cgatcttctg
    15481 ttaagttgac tctcacgtta catgacacag aaggaaaaat agagaaagct ggtacagaat
    15541 acgtttttgg cccactggat tatcaaattt cagaaggttt ctattctaca ttgatggatt
    15601 taatccgcct tgatgtgttt aaagaaacgg atgaaactat gagtgtggaa gagtcttatc
    15661 gaaacgaaaa acttttacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga
    15721 atcaagggag tgaagtcgaa gtaaatgaag aagtaccatg ggaacgacca atatttccaa
    15781 agaaggagtc aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc
    15841 agaaatcgat atttgagaaa gagaacgatg tgatgggtca gttggaggta gatccctttg
    15901 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    15961 aagagtcaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    16021 aagtagaaaa aaacgaaata gagatacaaa aggcaactgt atttgattat attttggatc
    16081 aatatccgcc agagcatgaa tggatgaaag aaaaaaacaa attattttta aatgagcttt
    16141 attcgaaata tcaattaccc caaacctatc aagcattttt agagagtaaa gaagaattaa
    16201 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    16261 atgagattgc acaagaagat ttattagcca cctttgaaga acgtcaacaa gaaacagatc
    16321 aagaaattaa tgagtatgcg acggaacagt cgtctaattg ggagaaagca aaagaggaac
    16381 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcgatacg
    16441 aaaagaaacg cgacaatgcg tattccttgt gtgtagaaaa gatcaataat tttactcaga
    16501 cgaatcaaga agcgttatcg aaagaaaaag aaaacttgtt aaaagaacgt ataagagaac
    16561 gaaaagaaga attttatcaa ggtttgcgta cagataagct aaagattagc aaagaattaa
    16621 atgagcaact gaataagctc tatcaaataa caagtgaagc tcttatggaa aaacacgagc
    16681 agatccagca agaattacaa gtacaaattc caaaatggaa aaaagagtat gaaattcaac
    16741 aacaagaatt gaaagaacaa gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat
    16801 tagaactaaa aaaacgtgaa caggatcttg cggaacaaca aatggcaata gaaaaacaac
    16861 ggttaaacca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    16921 atacattacg acaagctcag ttaaattatc tagcacaacc tcaaccaacg ccttcagtag
    16981 ctgctgttca aaccccaatg aacaaccaaa caaatctaac tccttcacca cagcgttcct
    17041 ttaacggatg gatggtggga tgtattgcaa gtttaagctt gcttctaggt ggagggacag
    17101 tatttgcttt taatcatatc aatcaatctt cacaggctga ttctgtagcc acaacggcgt
    17161 caatcaagga agaagcccaa gaacaagcaa gaaaagagta tgaacaacaa ttggaagaat
    17221 tattgaatcg taccaatcag gcaacaacag agtcaagtat ggaaaatagc aaagagagtg
    17281 atcctagtaa ggctaaagaa ggatcaagcg attcaactgt acaatcaaac acttccgaaa
    17341 gttcagaatc aaatgaggtg aaataatgcg tgttagagaa acaacgagac gaaaagacaa
    17401 acgattcaat ttagaaaaga ataggtatct ttctcatttc tatcgctata ctccttcagg
    17461 agaagagcta ccagaaaaac atgacaaacg tatttctcaa aagatttttt atagtggctt
    17521 agcttatctt cctttaaact atgttgttac aagaatacca gaagcatttc atcgatttga
    17581 gattattcca ggaagtttac aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt
    17641 attccatttt gattcaactg gtttttggaa tcctttgacc ccaacaacaa gtttaggcac
    17701 acgtttagtt gtttctacga tttgtgttgt tccagctata cttatgggat tgaaaatgga
    17761 catgtggttt cgtcctctag cagatgggca aaaaggagat aaccgattaa aaacgttaaa
    17821 ggaaatccaa caaatttatc cgcaaatacc agaatcaaag ggaggttttc ctggttatgg
    17881 cggaatacca attactcatt ataaaaagta ctggtatatt cagacggata cagtaaatac
    17941 ttgtattgtt ggtacttccc gttcagggaa aggacagata gaagttttag caaccattga
    18001 caatttgtct cgtgcagaaa gacaaagttc aatggttgtt aatgatccaa aaacagaatt
    18061 atatgttgct agtaaagatg aactagaagc tagagggtat gatgtgtatg catttaatat
    18121 caccgatcca ctgcaatcaa tgagtgataa tccattggca ctcatcgtga aatactggaa
    18181 gcgaggagac atagatactg caacgcagtt aaccaatact tttacgaata cgatttatta
    18241 tgatgccaat gcttcagaca acaaatactt taacgaaaat gcgcagaaag ctgtaaatgc
    18301 gcttattttt gcgttgttag aacaagccga tcaaacaggg gattactcca aattaacacc
    18361 caataatctt gttgagcttc tttctgaaat tggcggtttt aattatcaag atcctgagaa
    18421 tgattacaga caactcaatg ccttagatga attcatgaat cagttaccac ctggaaacat
    18481 agcaaaaaaa caatatgggg caacaaaaat tggttctgat aaagcaaaag ggaatatttt
    18541 atcgacggca atcacaggat tgaatccatt taccctaacg aaaattgcga aaatgacttc
    18601 tcaatcaacc attgattata agtccattgg ttttccgaag tatttggatc tcaaactaca
    18661 cgagagtctg ttaaatcagc gaattgttgt agagttttat catcaaggga aaaaatttca
    18721 tgaggaacgt gtcaaagtag gttatagagg tttttgtgaa atcaactttg attgtcattt
    18781 agagcaggga gatcatgttt gcttaaaata cgatcatcaa ggaaaagaat atgttgcttg
    18841 gtatgagttt tctcaaagag tattgaaaga cgaagaggga aatatcgttt atcgaaaaaa
    18901 acgaggagaa acccatttac cagaattaga aaaagaagct gttctaacat tagatgagga
    18961 taaaagtaac ttattagtag agtctattcg gatgcactat accgataaac caatggctat
    19021 ttttatgctc acacctgatt atgatccatc taaccatatc attgttagta tttttcttag
    19081 tcagttgtat aaagaattat ctacgcaatg tgttaagact aaaggagata aatgtcaccg
    19141 taggattcat tttattttag atgaatttgg gaatatgcca ccaatggata atatggaagg
    19201 aatcatgaca gtcacagctg gtagaaacat gttgtgggat ctgtttattc aatcctatca
    19261 gcaactttat gcgaagtatg gggaacaaaa cgcagcgatt atcaaagaaa attgtcagac
    19321 acatatttat attatgtcga cagacgataa taccatcgaa gaattttcca aaaaagtggg
    19381 gaataagacg gttgaacaag agaacagtac caccaattca ttaggtatga atactcatat
    19441 caatcgcaat gttgattcag atcgtatctt aactcctgaa agaatttcta cttttttaga
    19501 aggtgaaacg attgtactcg ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg
    19561 gccgtatcct atttttaatc atggggaaac gaatcttcct tatgcttatc aatatattga
    19621 agatttcaat ccatcgtggg atatcaatga tatagacata cattgtacac atgcggactt
    19681 ggatcttaag gctttacaaa ttgattacga ttattttcta cctgatatct atgcacgtcg
    19741 actttatcac aatatgcacg atcaaataga agaggaagct aaagaaaaca tttttattaa
    19801 aatcgttcaa tctttagaca actcaacggc acaaaagtta cttattcaat tttatgaaga
    19861 gaagcaaata gatcgtttgc aagaatacct tgaaaaattg catcaaaatg aagaaattac
    19921 agaggttata aggaaacgac ttttgctaga agctgaaaag gcggtgaaag aatgatcatt
    19981 ggaggaatat tttccggcat tgccaactgg tggtcaggct tatttgggga tggggatgca
    20041 gatcccaaaa ccgtaacgaa agtcttacaa gattatggta attatctgca gtatcaagat
    20101 gtattcagtt ggatttgtaa tactttttta tggggtttga ttaaagggtt atataatctc
    20161 aatactttac tagaaaaaac aatttatcaa agctttgatt taaaagactt attgaacgct
    20221 gctggtgtta atgaactgtt tcaaagtttg attagtaagg tattggctct tttttgtgtc
    20281 gtcacattaa tttatttagg cttaaaattt tttatgagta aacatccacc aaaaatcaaa
    20341 aatatcttgg ttaatctatt tatggccatg attttgattt ttggtggtag ttctttaatc
    20401 gatgaagggc taaatattag taaaaacttt tacggagacg tgactgcagc aaataaagca
    20461 gataaaactg gatcacctgc ttttcaaatt attcaaaata atgtgatcga tgtgagtacg
    20521 ctactagaaa ctgatccgaa taaagtagaa tccctaccaa cagataaaag aaatgctctc
    20581 acggcgaaaa actttaagac agcaaatatt aattcagtga ttattcctaa ggaagccgct
    20641 aagatggcgg atgaagataa gagcggtcta tcagatatac aaaaggatcg gctaagagcg
    20701 ttacaatacc gactggaact ggatgataat ggaaaagaaa tccctgtaga gattagtgat
    20761 gaggggttag caaaatatgt gtatacttca ggctatcgcc gatacattag tagtccaggg
    20821 attattttag ctggtgaatc ttctttgtcg gttgcctatt tatttatctt gtttacgatt
    20881 gtaacttgca ttattgagct ggtatttaaa aaattctatt tagtaattgc cgcgtcaacg
    20941 gactttgaga caggacaacg gatgaaaagt gctatagaag atatttctca atccttcttg
    21001 ttattggcgt ttacgatttt agaattacga atctatacat taatgctgtc aggtatcggt
    21061 gatttgcacg cagcaggcaa gatcaacgga tttttgtatg ttgtcgcttt aattgtacta
    21121 accattgcgc tattcaaagg ctctcaatct gttacgaaga ttttcggtgt tgatacaagt
    21181 ctgaaaaacg gcagcaattc tcttcttagc atgtttgcct taggaaatat tgcgaaaaat
    21241 gtgggtagtg gggctaaaaa ccttgcaagt gctggtaaga gtggtttaag caatttaaat
    21301 gatattcgta aaaatggatt tggcaatcga aaagatgtta ttcctgataa cggggaagaa
    21361 caaccagacg ataaagctac aattgtacct aatagtaaga aaacagtttc cgaaatgttc
    21421 gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt
    21481 gggttaaaag gaacagctga agatcttaca gaaaaagcca aagacacctt agaagagaaa
    21541 ttagacggca cgagagctaa agaattggct gaagctgtga agaatccatc cgaagcacta
    21601 gaaaaacaaa aagaaaaagc ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa
    21661 ggacaagtca atgcagcagt gaaggcaaat acaacaagtg aaaaagaaaa tcaagaaggt
    21721 gcagcgattc cttcagccaa agatcaccaa gatgagttac attctaacga aaattcgatc
    21781 ggagaaaaag attcagaaga aggacaacat ggacagttac caaaaaccga tccagaacaa
    21841 tatcgttata aatctaacat taattctgaa aatgaaaagc gcatttcaga tttaccaaaa
    21901 cgaaatcaag aagtacaaac ggatgaggat tccatcgatt tgccaaatga taatggaatc
    21961 gaagttggaa ctgaaaaatc aatgaaaatt ggtttgccta agacacaaaa tttaccaatg
    22021 aaagatagtc gtttgaatca attcaaggag aatgatcaaa gctttgttca ccaatcaact
    22081 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    22141 tcttacaatt tatctgaaag tggtgagaca gggaatgtgt ccattcaaaa tgttgtgaca
    22201 agagaagctg caccagaacc tattcaaaaa gttcacacag gttacaaacc agcaaatgtg
    22261 gcatttactg caccaacaaa aataaatgta acaagagaaa ctaaaaacag gaacaatgaa
    22321 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacgag
    22381 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tattaacatg
    22441 aaaaatcctc caagatttag tggggaatgg aagtttttcg cctttttctt tttagatcta
    22501 ttaacgcttg gtggttgctt tttttgggcg aaaatagtca ataatcttgt ccacttacca
    22561 ctcttttttg cactcttgaa ttatggctta tggattgcga tagctctagt gattgtttgg
    22621 cgaccttata ataacccagg tcaacgacaa atttttgcga ttatcgaatc tattttcttt
    22681 gcggataaga accactatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    22741 gaggatgaat aaatgcgagt caaaggacaa aattcagttg ataagaaatc cattttgcag
    22801 tttggaaaac cagaagaacg atctatcttg gatttatttc cttataaatc ctgtacagaa
    22861 gagggagtct tagttactaa agaagatcat tttcaacgtt attatcgtgt agtttctaca
    22921 gacgtggaag gattaaacga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    22981 atgcgtacgt atgtgcaaac aattaagtta gttagtttga ccacagagac ggatctaacg
    23041 gaacaaatcg tacaaaagcg gcaattatta gataagaatc gtatggaaca atccttagga
    23101 agaaatctac agcgattaag aaaatatgaa aagaaattag tagaggaaat ccaggaattg
    23161 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    23221 atcaaggatt tacaaaccag aaatcgtcag ttaatgcgat ctagtggcat tttagggtta
    23281 aaaccactct acaaaaaaga attgatcaag attctttatc gaatgaacaa tatgaatgat
    23341 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa atgatgttcc
    23401 gaaggaagag atcactagaa aattttctca cactttaagc ctgggtaagt tgggaaaagg
    23461 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagcg
    23521 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    23581 ggaaatcggg aaatagaaat tttaaaatcc aaagggtatg atttagattt tttaaaacaa
    23641 gtgatgccac aaggtggatt aaatttcgat gaagagtatg tgacctatgg aaatggttat
    23701 tcacgatgtg tgacagtcta tcactataca aaaaatccta ctcttttttg gttggccaat
    23761 ctcatgaaca atcctcatac agtggccaca ctagatgtta gcacagatga aaagcaaaaa
    23821 gtgattgatc aaatcaaccg cgcattagat gaattaaatg atcgatcaga taatgaacga
    23881 aaagcgactg accgaaataa aagtgcggaa gaatacctgg aactagcgga atatgcccga
    23941 aaattgactt ctaatgggga aatttctaaa caagtcaaag tgagagtaca tgtttacgga
    24001 aatacgatac aagaagtgga tgaacgggcg caggaactga tcgaaaatct aaaagggatg
    24061 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    24121 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    24181 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg aggtacacaa
    24241 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    24301 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    24361 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    24421 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    24481 gacgggatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagg
    24541 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaacg
    24601 cacttagaca aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    24661 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgt
    24721 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg taaagatcca
    24781 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    24841 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    24901 gagacgtatg gcatgatttt tgatggtcac agtacgattc gacaccttga ccaacaacag
    24961 ttggtctttt ttgacattga ctctattaca ggtttagatc gaggggtgca acaatgtcaa
    25021 atgtacatgg ctatgacgtt gatttggaat caggcattga tccagggaag aagacaacga
    25081 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    25141 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    25201 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    25261 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    25321 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    25381 tctttagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    25441 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    25501 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    25561 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    25621 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    25681 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    25741 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    25801 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    25861 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    25921 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    25981 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    26041 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    26101 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    26161 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    26221 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    26281 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    26341 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    26401 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    26461 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    26521 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    26581 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    26641 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    26701 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    26761 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    26821 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    26881 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    26941 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    27001 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    27061 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    27121 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    27181 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    27241 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    27301 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    27361 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    27421 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    27481 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    27541 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    27601 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    27661 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    27721 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    27781 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    27841 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    27901 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    27961 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    28021 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    28081 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggactagtgg gtaaatcaat
    28141 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaactt
    28201 tggatcaaaa gaaaccattc gcgttcattt tagtccgaac aaacgtcaat taaaattgtt
    28261 tgatggagga aaataatgga aaatcaagat gaaagtttaa tcaagcagta tgttaagcgt
    28321 cgcgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    28381 ttgatcactg tgtttgttac cctatttcta atacttgcgg cgggttcgat tgataattcg
    28441 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    28501 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    28561 ggtgcagcag tgaaatataa attattacca agtgtgattc tttcgcaata cggttatgaa
    28621 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    28681 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    28741 tggtacatga agtttcccaa ctcaaaagct gcattctcct attacggctt tatggtagcc
    28801 acacaatcaa attttaatgc atgtgtagga aataaaagcc ctggcgcaag cttattgatc
    28861 ttaggacgag gtggttatgc cgctgcagga attactgaag atagtccgta ttatacaggt
    28921 tgtatgtcga ttattacctc aaataaatta acagaatatg atgaatttgc gatcaaacat
    28981 tggggagaag gcggaaatga caacggtacg attactggtg aatggacgaa tcctttccct
    29041 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    29101 ggtgaattcc gtccgaatgg tttccatgat ggattagact ttggttcagt tgatcatcca
    29161 ggaagtgaga ttcatgcggt tcatggagga aaagtcgtgt acgttggaaa tcctggtatt
    29221 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgacg gcttgaatat ggtatatcaa
    29281 gaatttgcaa atagtacagg aaattcccgt gtgaaagtgg gagatcaagt gaaagttggt
    29341 caagtaatcg ggatacgaga tacagcacac ttgcatttag gcttcactcg aatggattgg
    29401 cgtcaagcac aaggacacgc attcacagat gatggtacat ggatcgatcc attaccattt
    29461 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    29521 gtcttaacag ctgcactcag cattagctta attggcaata tcgcgctctt ttggcagctg
    29581 aaagaggaac aagcgacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    29641 aagctcaaaa aaaatttctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    29701 aatgagaaaa accaagaaat cgatcagctg aagcaacaag acggtataaa agcaaaagaa
    29761 gaagtcacag atgcccaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    29821 tcactaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    29881 gacaaattaa gtcctgcaga tgatgatcac caacatgatg attatggaag ttactccttt
    29941 acattaggtg acgtgcaaac ttacgctgaa acaagcagtg taaaagagga acaaaatttt
    30001 gtcgtatttg ttgattatac aatcggcaat cctcaattca aagatgtcaa accgcaaaaa
    30061 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
    30121 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
    30181 ttttaaaaaa cggaaaagtt atcttagatg gctgggaatc tttacgattc tcggcttttt
    30241 gattcttgct agtccgattt atacgcataa gaaggtaaac ataccagatg aggtaagcac
    30301 gaacgctttt ttaaacacga aagaactgac aatggtatca aaagaaaaag gagcgaatca
    30361 gcaattagtt tttgaatttg cgattgacga tgaggatcaa ggcttattaa aagaactggc
    30421 taatcttgat tatcgcgtag aagtaaagac agctaaaggt gattatcaag caataaaagc
    30481 caaagtgatc aaggtgtctg atgattattt tgtagtgaaa ttagcacatg taccagaaga
    30541 atatatcgcc atgcgtttaa ccattgttcc tgaaaaaatt gatccaaaag tggatatgca
    30601 agaaccacaa gatctgattt attacgttca cgaagataag gttaaggatc aagtgaaaga
    30661 tgataattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    30721 agcagcacaa aaacagattg aatccttgca agcaaccatt gatttgaatg aagaattagt
    30781 gaataaacta aaagaccaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    30841 taagattaat gattaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
    30901 agagatcatt caaaaaattc aagaaaaaat tgatcgtatg acaaaagaaa atatttagta
    30961 gaacaggtgg agtgaaagaa atgaagggag cgaacttggt cggcaaagat attttcgctg
    31021 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcaag
    31081 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    31141 ccttaaaaat cagatcattt gaagctttgt atttagctgc taaaaaatat tttacggctg
    31201 aaagaaaata aaaacaggag gattttatgt
//
LOCUS       DACIWG010000035        29109 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.035, whole genome shotgun sequence.
ACCESSION   DACIWG010000035 DACIWG010000000
VERSION     DACIWG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.035"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1030)
                     /locus_tag="IYF54_001678"
     CDS             complement(<1..1030)
                     /locus_tag="IYF54_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9832009.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IYF54_001679"
     CDS             complement(1112..1801)
                     /locus_tag="IYF54_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ9832010.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYF54_001680"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYF54_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ9832011.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IYF54_001681"
     CDS             complement(2328..2831)
                     /locus_tag="IYF54_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ9832012.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYF54_001682"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYF54_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ9832013.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYF54_001683"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYF54_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ9832014.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYF54_001684"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYF54_001684"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ9832015.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYF54_001685"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYF54_001685"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ9832016.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IYF54_001686"
     CDS             complement(6443..7462)
                     /locus_tag="IYF54_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ9832017.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IYF54_001687"
     CDS             complement(7635..8522)
                     /locus_tag="IYF54_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9832018.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYF54_001688"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYF54_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ9832019.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYF54_001689"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYF54_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ9832020.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYF54_001690"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYF54_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ9832021.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IYF54_001691"
     CDS             9753..10397
                     /locus_tag="IYF54_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9832022.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IYF54_001692"
     CDS             complement(10453..10956)
                     /locus_tag="IYF54_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ9832023.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IYF54_001693"
     CDS             11553..12518
                     /locus_tag="IYF54_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ9832024.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IYF54_001694"
     CDS             complement(12617..13789)
                     /locus_tag="IYF54_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832025.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IYF54_001695"
     CDS             complement(13776..14921)
                     /locus_tag="IYF54_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832026.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IYF54_001696"
     CDS             complement(14935..15867)
                     /locus_tag="IYF54_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832027.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IYF54_001697"
     CDS             15984..17063
                     /locus_tag="IYF54_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9832028.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IYF54_001698"
     CDS             17060..17689
                     /locus_tag="IYF54_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832029.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IYF54_001699"
     CDS             complement(17799..18026)
                     /locus_tag="IYF54_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9832030.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IYF54_001700"
     CDS             complement(18129..20717)
                     /locus_tag="IYF54_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ9832031.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IYF54_001701"
     CDS             complement(20737..21396)
                     /locus_tag="IYF54_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9832032.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IYF54_001702"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IYF54_001702"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ9832033.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IYF54_001703"
     CDS             complement(22169..23035)
                     /locus_tag="IYF54_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9832034.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IYF54_001704"
     CDS             23144..23785
                     /locus_tag="IYF54_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ9832035.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IYF54_001705"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IYF54_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ9832036.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IYF54_001706"
     CDS             complement(25226..26125)
                     /locus_tag="IYF54_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ9832037.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IYF54_001707"
     CDS             complement(26127..26924)
                     /locus_tag="IYF54_001707"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ9832038.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IYF54_001708"
     CDS             complement(26902..27606)
                     /locus_tag="IYF54_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ9832039.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IYF54_001709"
     CDS             27737..28312
                     /locus_tag="IYF54_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ9832040.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="IYF54_001710"
     CDS             28430..29107
                     /locus_tag="IYF54_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ9832041.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6168 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttagctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DACIWG010000036        28346 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.036, whole genome shotgun sequence.
ACCESSION   DACIWG010000036 DACIWG010000000
VERSION     DACIWG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.036"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(55..432)
                     /locus_tag="IYF54_001711"
     CDS             complement(55..432)
                     /locus_tag="IYF54_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832042.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            688..1416
                     /locus_tag="IYF54_001712"
     CDS             688..1416
                     /locus_tag="IYF54_001712"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ9832043.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1416..1670
                     /gene="purS"
                     /locus_tag="IYF54_001713"
     CDS             1416..1670
                     /gene="purS"
                     /locus_tag="IYF54_001713"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ9832044.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1672..2343
                     /gene="purQ"
                     /locus_tag="IYF54_001714"
     CDS             1672..2343
                     /gene="purQ"
                     /locus_tag="IYF54_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ9832045.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2344..4566
                     /gene="purL"
                     /locus_tag="IYF54_001715"
     CDS             2344..4566
                     /gene="purL"
                     /locus_tag="IYF54_001715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ9832046.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4551..5990
                     /locus_tag="IYF54_001716"
     CDS             4551..5990
                     /locus_tag="IYF54_001716"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ9832047.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6022..7065
                     /locus_tag="IYF54_001717"
     CDS             6022..7065
                     /locus_tag="IYF54_001717"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ9832048.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7062..7640
                     /gene="purN"
                     /locus_tag="IYF54_001718"
     CDS             7062..7640
                     /gene="purN"
                     /locus_tag="IYF54_001718"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ9832049.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7637..9172
                     /gene="purH"
                     /locus_tag="IYF54_001719"
     CDS             7637..9172
                     /gene="purH"
                     /locus_tag="IYF54_001719"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ9832050.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9188..10462
                     /gene="purD"
                     /locus_tag="IYF54_001720"
     CDS             9188..10462
                     /gene="purD"
                     /locus_tag="IYF54_001720"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ9832051.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10584..11498
                     /locus_tag="IYF54_001721"
     CDS             10584..11498
                     /locus_tag="IYF54_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ9832052.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11631..13196)
                     /locus_tag="IYF54_001722"
     CDS             complement(11631..13196)
                     /locus_tag="IYF54_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9832053.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13189..14640)
                     /locus_tag="IYF54_001723"
     CDS             complement(13189..14640)
                     /locus_tag="IYF54_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9832054.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14654..15670)
                     /locus_tag="IYF54_001724"
     CDS             complement(14654..15670)
                     /locus_tag="IYF54_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9832055.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15890..16978
                     /locus_tag="IYF54_001725"
     CDS             15890..16978
                     /locus_tag="IYF54_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9832056.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17032..18558
                     /locus_tag="IYF54_001726"
     CDS             17032..18558
                     /locus_tag="IYF54_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832057.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18560..19765
                     /locus_tag="IYF54_001727"
     CDS             18560..19765
                     /locus_tag="IYF54_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9832058.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20022..20999
                     /gene="guaC"
                     /locus_tag="IYF54_001728"
     CDS             20022..20999
                     /gene="guaC"
                     /locus_tag="IYF54_001728"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ9832059.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21263..22687
                     /locus_tag="IYF54_001729"
     CDS             21263..22687
                     /locus_tag="IYF54_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ9832060.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23148..24257
                     /gene="pdhA"
                     /locus_tag="IYF54_001730"
     CDS             23148..24257
                     /gene="pdhA"
                     /locus_tag="IYF54_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ9832061.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24260..25237
                     /locus_tag="IYF54_001731"
     CDS             24260..25237
                     /locus_tag="IYF54_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ9832062.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25262..26905
                     /locus_tag="IYF54_001732"
     CDS             25262..26905
                     /locus_tag="IYF54_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAQ9832063.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26913..28319
                     /gene="lpdA"
                     /locus_tag="IYF54_001733"
     CDS             26913..28319
                     /gene="lpdA"
                     /locus_tag="IYF54_001733"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ9832064.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         9208 a         4879 c         6421 g         7838 t
ORIGIN      
        1 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
       61 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
      121 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
      181 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
      241 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
      301 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
      361 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
      421 tgcatcttcc atcggcaagt gcctcctcga acccattaac caaattttta gttaattcta
      481 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
      541 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
      601 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
      661 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
      721 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
      781 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
      841 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
      901 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
      961 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
     1021 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
     1081 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
     1141 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
     1201 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
     1261 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
     1321 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
     1381 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
     1441 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
     1501 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
     1561 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     1621 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     1681 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     1741 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     1801 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     1861 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     1921 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     1981 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     2041 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     2101 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     2161 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     2221 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     2281 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     2341 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     2401 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     2461 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     2521 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     2581 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     2641 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     2701 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     2761 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     2821 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     2881 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     2941 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     3001 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     3061 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     3121 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     3181 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     3241 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     3301 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     3361 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     3421 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     3481 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     3541 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     3601 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     3661 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     3721 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     3781 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     3841 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     3901 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     3961 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     4021 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     4081 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     4141 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     4201 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     4261 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
     4321 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
     4381 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
     4441 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
     4501 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
     4561 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
     4621 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
     4681 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
     4741 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
     4801 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
     4861 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
     4921 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
     4981 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
     5041 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
     5101 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
     5161 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
     5221 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
     5281 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
     5341 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
     5401 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
     5461 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
     5521 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
     5581 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
     5641 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
     5701 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
     5761 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
     5821 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
     5881 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
     5941 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
     6001 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
     6061 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
     6121 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
     6181 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
     6241 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
     6301 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
     6361 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
     6421 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
     6481 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
     6541 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
     6601 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
     6661 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
     6721 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
     6781 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
     6841 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
     6901 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
     6961 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
     7021 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
     7081 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
     7141 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
     7201 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
     7261 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
     7321 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
     7381 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
     7441 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
     7501 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
     7561 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
     7621 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
     7681 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
     7741 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
     7801 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
     7861 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
     7921 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
     7981 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
     8041 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
     8101 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
     8161 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
     8221 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
     8281 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
     8341 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
     8401 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
     8461 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
     8521 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
     8581 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
     8641 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
     8701 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
     8761 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
     8821 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
     8881 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
     8941 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
     9001 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
     9061 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
     9121 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
     9181 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
     9241 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
     9301 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
     9361 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
     9421 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
     9481 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
     9541 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
     9601 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
     9661 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
     9721 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
     9781 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
     9841 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
     9901 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
     9961 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    10021 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    10081 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    10141 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    10201 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    10261 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    10321 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    10381 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    10441 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    10501 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    10561 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    10621 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    10681 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    10741 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    10801 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    10861 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    10921 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    10981 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    11041 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    11101 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    11161 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    11221 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    11281 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    11341 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    11401 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    11461 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    11521 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    11581 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    11641 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    11701 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    11761 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    11821 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    11881 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    11941 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    12001 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    12061 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    12121 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    12181 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    12241 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    12301 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    12361 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    12421 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    12481 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    12541 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    12601 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    12661 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    12721 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    12781 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    12841 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    12901 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    12961 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    13021 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    13081 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    13141 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    13201 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    13261 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    13321 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    13381 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    13441 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    13501 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    13561 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    13621 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    13681 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    13741 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    13801 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    13861 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    13921 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    13981 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    14041 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    14101 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    14161 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    14221 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    14281 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    14341 tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc
    14401 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    14461 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    14521 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    14581 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    14641 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    14701 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    14761 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    14821 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    14881 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    14941 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    15001 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    15061 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    15121 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    15181 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    15241 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    15301 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    15361 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    15421 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    15481 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    15541 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    15601 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    15661 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    15721 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    15781 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    15841 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    15901 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    15961 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    16021 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    16081 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    16141 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    16201 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    16261 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    16321 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    16381 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    16441 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    16501 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    16561 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    16621 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    16681 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    16741 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    16801 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    16861 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    16921 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    16981 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    17041 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    17101 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    17161 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    17221 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    17281 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    17341 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    17401 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    17461 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    17521 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    17581 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    17641 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    17701 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    17761 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    17821 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    17881 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    17941 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    18001 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    18061 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    18121 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    18181 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    18241 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    18301 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    18361 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    18421 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    18481 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    18541 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    18601 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    18661 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    18721 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    18781 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    18841 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    18901 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    18961 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    19021 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    19081 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    19141 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    19201 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    19261 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    19321 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    19381 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    19441 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    19501 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    19561 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    19621 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    19681 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    19741 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    19801 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    19861 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    19921 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    19981 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    20041 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    20101 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    20161 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    20221 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    20281 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    20341 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    20401 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    20461 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    20521 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    20581 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    20641 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    20701 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    20761 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    20821 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    20881 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    20941 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    21001 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    21061 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    21121 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    21181 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    21241 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    21301 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    21361 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    21421 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    21481 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    21541 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    21601 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    21661 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    21721 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    21781 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    21841 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    21901 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    21961 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    22021 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    22081 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    22141 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    22201 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    22261 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    22321 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    22381 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    22441 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    22501 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    22561 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    22621 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac
    22681 agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag
    22741 ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    22801 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    22861 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    22921 tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    22981 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    23041 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    23101 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    23161 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    23221 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    23281 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    23341 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    23401 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    23461 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    23521 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    23581 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    23641 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    23701 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    23761 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    23821 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    23881 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    23941 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    24001 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    24061 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    24121 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    24181 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    24241 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    24301 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    24361 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    24421 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    24481 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    24541 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    24601 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    24661 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    24721 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    24781 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    24841 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    24901 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    24961 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    25021 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    25081 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    25141 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    25201 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    25261 catggcttat caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt
    25321 caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca
    25381 aaatgacaaa tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat
    25441 tgttccagaa ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg
    25501 acacgaagac aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc
    25561 ggctgctgaa cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca
    25621 atttaagtta ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt
    25681 caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc
    25741 agtagaagaa attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg
    25801 cactgtagca aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc
    25861 accgtcaact agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc
    25921 agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg
    25981 tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg
    26041 cagagtaaca aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc
    26101 gaaatcagaa gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa
    26161 accagctgca ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc
    26221 aatgacgcca acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc
    26281 acacgtgaca ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt
    26341 taaagaagtt gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc
    26401 tttaacagcg actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca
    26461 agaaatcgtt tataaacatt actacaatat cggtattgca actgatacgg atcacggact
    26521 atatgtacca aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat
    26581 caatgagaaa gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg
    26641 aacaattacg atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa
    26701 ctatccagaa gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc
    26761 tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat
    26821 cgtagatgga gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc
    26881 agagctatta atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat
    26941 tagatacagt tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg
    27001 aaatgggaca aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg
    27061 tcggctgtat tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag
    27121 attctgaaat gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag
    27181 aatggaaaga aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa
    27241 aacataaggt tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg
    27301 tcattcatcc agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt
    27361 cccgtccaat cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg
    27421 gattagcatt gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg
    27481 ccgaattggg cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac
    27541 cacaaatttt accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga
    27601 aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca
    27661 gcgtgacagt gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga
    27721 tggtcactgt aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg
    27781 aagtaggaga acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca
    27841 tctatgccat cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag
    27901 caaaaatcgc tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc
    27961 cggcagtagc cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa
    28021 aagatgctgg tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat
    28081 tatcgcttgg taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc
    28141 ttattggcgc gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag
    28201 caatcgaatc aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc
    28261 ttggcgaaat cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat
    28321 ttgaataata caggcgaaac gcaact
//
LOCUS       DACIWG010000037        26238 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.037, whole genome shotgun sequence.
ACCESSION   DACIWG010000037 DACIWG010000000
VERSION     DACIWG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.037"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYF54_001734"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYF54_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ9832065.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IYF54_001735"
     CDS             1995..4661
                     /locus_tag="IYF54_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9832066.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IYF54_001736"
     CDS             5025..5777
                     /locus_tag="IYF54_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9832067.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IYF54_001737"
     CDS             complement(5799..6953)
                     /locus_tag="IYF54_001737"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ9832068.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IYF54_001738"
     CDS             7138..9579
                     /locus_tag="IYF54_001738"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ9832069.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IYF54_001739"
     CDS             complement(9909..10613)
                     /locus_tag="IYF54_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832070.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IYF54_001740"
     CDS             complement(10800..11045)
                     /locus_tag="IYF54_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832071.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IYF54_001741"
     CDS             complement(11062..11913)
                     /locus_tag="IYF54_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ9832072.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IYF54_001742"
     CDS             12079..13083
                     /locus_tag="IYF54_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ9832073.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IYF54_001743"
     CDS             complement(13324..13680)
                     /locus_tag="IYF54_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9832074.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IYF54_001744"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IYF54_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ9832075.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IYF54_001745"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IYF54_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ9832076.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IYF54_001746"
     CDS             complement(18040..18759)
                     /locus_tag="IYF54_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832077.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IYF54_001747"
     CDS             18968..19600
                     /locus_tag="IYF54_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ9832078.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IYF54_001748"
     CDS             19659..20519
                     /locus_tag="IYF54_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ9832079.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IYF54_001749"
     CDS             20626..23019
                     /locus_tag="IYF54_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ9832080.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IYF54_001750"
     CDS             23201..25042
                     /locus_tag="IYF54_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ9832081.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IYF54_001751"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IYF54_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ9832082.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8467 a         4668 c         5482 g         7621 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaa
//
LOCUS       DACIWG010000038        23829 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.038, whole genome shotgun sequence.
ACCESSION   DACIWG010000038 DACIWG010000000
VERSION     DACIWG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.038"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(53..787)
                     /locus_tag="IYF54_001752"
     CDS             complement(53..787)
                     /locus_tag="IYF54_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ9832083.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1526
                     /locus_tag="IYF54_001753"
                     /pseudo
     CDS             1114..1526
                     /locus_tag="IYF54_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1519..2220
                     /locus_tag="IYF54_001754"
     CDS             1519..2220
                     /locus_tag="IYF54_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ9832084.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IYF54_001755"
     CDS             2220..3806
                     /locus_tag="IYF54_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9832085.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISPMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IYF54_001756"
     CDS             3825..4046
                     /locus_tag="IYF54_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ9832086.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IYF54_001757"
     CDS             4043..4804
                     /locus_tag="IYF54_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ9832087.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IYF54_001758"
     CDS             5184..5693
                     /locus_tag="IYF54_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ9832088.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IYF54_001759"
     CDS             complement(5768..6307)
                     /locus_tag="IYF54_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ9832089.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYF54_001760"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYF54_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ9832090.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IYF54_001761"
     CDS             7374..8171
                     /locus_tag="IYF54_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ9832091.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IYF54_001762"
     CDS             8199..8834
                     /locus_tag="IYF54_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ9832092.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IYF54_001763"
     CDS             8854..9627
                     /locus_tag="IYF54_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832093.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IYF54_001764"
     CDS             10035..10832
                     /locus_tag="IYF54_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ9832094.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IYF54_001765"
     CDS             10810..11223
                     /locus_tag="IYF54_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832095.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IYF54_001766"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IYF54_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ9832096.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IYF54_001767"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IYF54_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ9832097.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IYF54_001768"
     CDS             16000..16560
                     /locus_tag="IYF54_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ9832098.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYF54_001769"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYF54_001769"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ9832099.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IYF54_001770"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IYF54_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ9832100.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IYF54_001771"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IYF54_001771"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ9832101.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IYF54_001772"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IYF54_001772"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ9832102.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IYF54_001773"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IYF54_001773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ9832103.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IYF54_001774"
     CDS             21907..22485
                     /locus_tag="IYF54_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ9832104.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IYF54_001775"
     CDS             22537..23019
                     /locus_tag="IYF54_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ9832105.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IYF54_001776"
     CDS             23136..23510
                     /locus_tag="IYF54_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832106.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5117 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct ccaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DACIWG010000039        23360 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.039, whole genome shotgun sequence.
ACCESSION   DACIWG010000039 DACIWG010000000
VERSION     DACIWG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.039"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            25..696
                     /locus_tag="IYF54_001777"
     CDS             25..696
                     /locus_tag="IYF54_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9832107.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IYF54_001778"
     CDS             755..1195
                     /locus_tag="IYF54_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832108.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IYF54_001779"
     CDS             1207..1875
                     /locus_tag="IYF54_001779"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ9832109.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IYF54_001780"
     CDS             1939..2562
                     /locus_tag="IYF54_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ9832110.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IYF54_001781"
     CDS             2618..3004
                     /locus_tag="IYF54_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ9832111.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IYF54_001782"
     CDS             3217..4608
                     /locus_tag="IYF54_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ9832112.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4955..5602
                     /locus_tag="IYF54_001783"
     CDS             4955..5602
                     /locus_tag="IYF54_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ9832113.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            5995..8640
                     /locus_tag="IYF54_001784"
     CDS             5995..8640
                     /locus_tag="IYF54_001784"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ9832114.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8950..10263
                     /locus_tag="IYF54_001785"
     CDS             8950..10263
                     /locus_tag="IYF54_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ9832115.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            10253..10906
                     /locus_tag="IYF54_001786"
     CDS             10253..10906
                     /locus_tag="IYF54_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9832116.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10961..11653
                     /locus_tag="IYF54_001787"
     CDS             10961..11653
                     /locus_tag="IYF54_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ9832117.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            11803..12003
                     /locus_tag="IYF54_001788"
     CDS             11803..12003
                     /locus_tag="IYF54_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9832118.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(12257..13936)
                     /locus_tag="IYF54_001789"
     CDS             complement(12257..13936)
                     /locus_tag="IYF54_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ9832119.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(13938..14150)
                     /locus_tag="IYF54_001790"
     CDS             complement(13938..14150)
                     /locus_tag="IYF54_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ9832120.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            14543..16273
                     /gene="efrA"
                     /locus_tag="IYF54_001791"
     CDS             14543..16273
                     /gene="efrA"
                     /locus_tag="IYF54_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ9832121.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            16270..18030
                     /gene="efrB"
                     /locus_tag="IYF54_001792"
     CDS             16270..18030
                     /gene="efrB"
                     /locus_tag="IYF54_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ9832122.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            18125..18322
                     /locus_tag="IYF54_001793"
     CDS             18125..18322
                     /locus_tag="IYF54_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832123.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            18475..18960
                     /locus_tag="IYF54_001794"
     CDS             18475..18960
                     /locus_tag="IYF54_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ9832124.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(19064..19657)
                     /gene="clpP"
                     /locus_tag="IYF54_001795"
     CDS             complement(19064..19657)
                     /gene="clpP"
                     /locus_tag="IYF54_001795"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ9832125.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            19837..20364
                     /locus_tag="IYF54_001796"
     CDS             19837..20364
                     /locus_tag="IYF54_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ9832126.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            20455..21192
                     /locus_tag="IYF54_001797"
     CDS             20455..21192
                     /locus_tag="IYF54_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ9832127.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            21196..22113
                     /gene="miaA"
                     /locus_tag="IYF54_001798"
     CDS             21196..22113
                     /gene="miaA"
                     /locus_tag="IYF54_001798"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ9832128.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            22115..23344
                     /gene="hflX"
                     /locus_tag="IYF54_001799"
     CDS             22115..23344
                     /gene="hflX"
                     /locus_tag="IYF54_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ9832129.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         7601 a         4005 c         5052 g         6702 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
     4861 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
     4921 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
     4981 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc
     5041 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
     5101 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
     5161 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
     5221 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
     5281 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
     5341 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
     5401 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
     5461 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
     5521 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
     5581 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
     5641 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
     5701 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
     5761 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
     5821 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
     5881 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
     5941 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
     6001 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
     6061 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
     6121 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
     6181 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
     6241 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
     6301 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
     6361 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
     6421 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
     6481 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
     6541 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
     6601 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
     6661 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
     6721 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
     6781 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
     6841 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat
     6901 gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt
     6961 aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc
     7021 cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa
     7081 tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa
     7141 gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc
     7201 catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac
     7261 aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta
     7321 caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg
     7381 ggctggccag atacggaaag cgaagattat aagcgatact tccctactag cactttagta
     7441 accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt
     7501 actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg
     7561 cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat
     7621 ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc
     7681 ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca
     7741 cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa
     7801 aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact
     7861 gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg
     7921 gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct
     7981 gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta
     8041 ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga
     8101 aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct
     8161 tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa
     8221 gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc
     8281 gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg
     8341 acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca
     8401 gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa
     8461 aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa
     8521 cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat
     8581 tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa
     8641 gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat
     8701 cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata
     8761 aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga
     8821 tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct
     8881 ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg
     8941 gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt
     9001 tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga
     9061 aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg
     9121 agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa
     9181 acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag
     9241 cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt
     9301 attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg
     9361 gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta
     9421 ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca
     9481 attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca
     9541 ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca
     9601 ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg
     9661 ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt
     9721 ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt
     9781 caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga
     9841 tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat
     9901 aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa
     9961 atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg
    10021 cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa
    10081 ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc
    10141 ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca
    10201 ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta
    10261 taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga
    10321 agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa
    10381 attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt
    10441 tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt
    10501 ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga
    10561 acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt
    10621 gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg
    10681 agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa
    10741 agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat
    10801 cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc
    10861 tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt
    10921 tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa
    10981 agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt
    11041 atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat
    11101 gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac
    11161 tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc
    11221 cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg
    11281 taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca
    11341 tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat
    11401 cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta
    11461 ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgtta ttccatcgca
    11521 gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt
    11581 actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat
    11641 ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta
    11701 attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt
    11761 tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta
    11821 aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta
    11881 ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca
    11941 gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct
    12001 taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga
    12061 gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtattt ttttgtatga
    12121 aaaagtaaat aaatttaaga ggctatgaaa gagccacctt gtatcaagga ataagaacaa
    12181 acaaaaatgg cttctcatgt ttttcatttg ttttctctta tattcaggac aaggtgctga
    12241 tttcataacc tcttatttat tcttcatctg gtgtcattac cattggcaaa atcattggat
    12301 gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt
    12361 tatattctga acagtcaggt ccattcaaac attctcttaa agcgtggaat aagattcgct
    12421 gagcttcatt gatcatttct cctgattctc gcatgtagac aaatccacga gagagaatat
    12481 ctggaccagc caagatttct tttttcttga tatccactgt cgcgacagct aatacaagac
    12541 cttcttctga aagcaggtga cggtcacgta aaacgacatt accaatatca ccgatcccgc
    12601 taccatcgat atacacatct ccagccacaa aatgcccagc aggacgcgca gaatcgctag
    12661 ttaacgctaa aacatcgcca ttttccatga tgaagcagtt ttctgcagga acacctacat
    12721 cttgtgctag agaagcatgt actttcaaca tgcggaattc tccgtggaca ggaataaaat
    12781 attttggctt catcaaacga agcattaatt tttgttcttc ttgtccaccg tgaccggaag
    12841 tatggatgtt attgattttt ccgtgaatca cttctgcacc agcttcagac agcaaattga
    12901 tcaaatgatt gacacttgtt gtattaccag gaataggtga acttgaaaag atcactgtat
    12961 cgtctggctg gatttggatt tgacggtgag taccattcgc gatgcggctc aacgcggcca
    13021 tcgcttctcc ttgagaacct gtacatagga tcatcgtttc attggcaggc aagcgattga
    13081 tttcgtgtgc atctacaaat gtgccgtctg gaactttgat atatcctaaa cgttgaccgt
    13141 tgacgatggc attttccatg cttcgtccga aaacagcgat tttacgacca gttgccacag
    13201 cggcatctgc tgcttgctgc aaacggaaga tgttagaagc aaagctggca aaaatgatac
    13261 gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt tctgatttag
    13321 taaatgttgg aatctcagca tttgtactat cagataaaag gcacaagact ccttcttccc
    13381 cgatacgagc cattttatgc aagtttgctg gttcgccaac aggagtgaaa tcgaatttga
    13441 agtctccagt cgctacgata tttccagaag gtgttttcac cacgatccct aatgcatctg
    13501 ggatactatg ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg atgatcgtat
    13561 cttcattgat ctcatggagc tctgcatctc gaagcaagcc atgctcatct aatttgtttg
    13621 tgatcaacgc taatgcgaga ggaccagcat agataggaat atttgcttgt ttcagcaagt
    13681 agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct tttactttgt
    13741 gtaagttttg tatgatgtag ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt
    13801 cttccgggaa tttgatcccg gcatcaatca aaatgatttc atcttggaat tgtaccccat
    13861 acgtattttt cccgatttca cctaatccac caatagcaaa gacgcctgtc tcgttatttt
    13921 tgatggaaag tttcatatta aaactccact aatgagaaat cagcattttc tttttcgtac
    13981 tctagatgac ttccttctag aagttgaacg aattcaatat tgtatggggt attttcttcc
    14041 acacgttggc gagcgtctac atcgcttttt gcctcaatgt aaagagattt tgtttcttcc
    14101 cgtttagggt tgcgtacttt tgtttcttga taataaactt tatagatcat tgttttgtct
    14161 cctttatctg cgtaaagcag tgatttagct agttgatttt tttagacaac acaaaacgag
    14221 gggacaagcc acctggtttt tcatatgctt tgactagaat actggattcg agtcaaagag
    14281 acaagcctcg tttttgctta tctgttcgga tattttttca accgtacctt ctgtataaaa
    14341 aatatacaac aattaattgt tattctagca taaaacagca aataagtata gaaatcaact
    14401 agattgaaag aaaaaatcaa tgtttatcat cccgctataa tcataatgac ttttttatga
    14461 gagaagtgtt ctattaatag gctcgttgtg ctatcatttt tagtaatgaa aatgtaacaa
    14521 aagattaaaa ggagatgaga aagtgaaact aatgtggcat tataccatgc gttataagaa
    14581 attcttgttt ttcaatttca tatgcgtatt tggttttatt ttgattgaat taggacttcc
    14641 tacgatttta gcacgtatga tcgatgtagg aatccgcaat gatgattttg attatgtgaa
    14701 gcaacaaggg atatttatga tcgttattac tgtaatcggt attattttga atatcttgtt
    14761 aggttatttt ggttcgagga ttacgactaa tatcgtagca gatattcgag atgatttgtt
    14821 cgaaaagatc caaagttatt cgcatcagga atacgaaaat ctaggcgtat cttcattgat
    14881 cactcgtaca acaaacgatg cttatcagat catgttgttc ctgcaaaata ttttacgtat
    14941 cggttttatg gcaccaatga tgttcattgt cagtttgtac atggtcatgc gtacaagtgt
    15001 gacactcagt ttgtatgtag taggtgcatt acctttacta ttattggcag tcgtagcgat
    15061 tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa aacttagata aaatcaacag
    15121 tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt gcattcgtca atgaaaagtt
    15181 tgaagaaaaa cgttttgctg tagtcaatga gaattatgct tctagttcaa aaagcttatt
    15241 ccgtttgatg gctgttgcac aaccgggttt ttatttctta tttaatatcg taatggtatt
    15301 gatcatctgg agcggggcag tgcaaatcga tgcaggcgag cttcgtgtag gagatttgat
    15361 tgcgtttatc gagtatatat tccatgcctt gttctcattc atgttatttg ctagtgtgtt
    15421 catgatgtat ccaagagcag ctgtttcagc tggacgtatc caagaagccc tagatgccaa
    15481 accttcgatt gtagaagatc caaatggtat atctgataca aaaactaaag ggtatatcga
    15541 atttcaaaat gtaacatttg cttatccagg ccattcgcaa gagccagtga tacgaaatgt
    15601 cagttttaca gcctctccag gagagaccgt tgcttttatt ggaagtaccg gtagcggaaa
    15661 atctacgttg atccaattga tccctcgctt ttatgatgtg agtgaaggaa agatcttgat
    15721 cgatggagta gacgtgagag attatcggct aagcaagttg cgagataaga ttgggtttat
    15781 tccgcaaaaa gcgctgctat ttactggaac gattgcagat aatttgcgat acgggaaaga
    15841 agatgctacg caagaagaaa tggaacgtgc agctgagatt gctcaagctt cagaattcat
    15901 ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa ggtggttcca atttttcagg
    15961 tggacaaaaa caacgattgg caatagccag agccatcata cgccgtccgg aaatctatat
    16021 ttttgatgac agcttttctg cattagatta tcaaacggat gcgaagttac gtgctcgtct
    16081 gaaaaaagaa acgacagaat ctactgtatt gatcgtagca caacgtgtgg gaacgatcat
    16141 gcatgcagat aagatcatcg tattaaatga aggagaagtc gtcggtatgg ggacgcatcg
    16201 ggagttgttg gaaaaatgcc cgatttatta tgatattgcc gcttctcaat tatcaaagga
    16261 ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt tagctcgata tatcagaccg
    16321 tatcgtgaga cttttctttt agtgatccta tttacagttc tgacagtggc tttcaacgtg
    16381 gctttgccat atgttacagg acttcctact acagaaatca gccgtaatat tgccaatggc
    16441 gaatcaatca atttttctta tatattcaaa tgtctcattt ggatcacagt tgttggaata
    16501 gggtattgta tttcgcaatt actttctggt gttttgatga caaatgtcgt tcaaagtgcc
    16561 atgcatgatt tgagaagaga catagaagaa aaaatcaatc gtcttccggt ttcatatttc
    16621 gataaaaacc agcaaggaaa tattttgtct cgtgtgacga atgatgtaga tgcagtcagc
    16681 ggtgctttac aacaagcttt tattggtatc gtaaatgcga tattgggaat tacgttggct
    16741 gcagcaatga tgttttatat ccagccgatg atggcgctga tctctatgat catgattcca
    16801 acatcgatct ggatctcaaa acgagtgatc aatgcttcgc agaaatattt tcaaagcatg
    16861 cagaattctc ttggtgagct aaacggctac gtccaagaaa atatgacagg ttttagtgta
    16921 ttgaaagtat atggacgcga aaaagaaaca ttggaaggat tcaaaaaagt taatcacagc
    16981 ttgaaatact ttgggtttcg tgcagcattc atttctggct tgatgatgcc tctagttcaa
    17041 ttaactgcct atgctactta tattggtatg gctgttctag gaagctttta tgttattact
    17101 ggggcgatcg ttgtcggtca actacaagca ttcatccaat atatttggca aatcagtcag
    17161 ccaatgggga acgtgacaca attgtcttca gttttgcaaa gtgcatcagc ggcaacgaaa
    17221 cgtgtattcg agattttaga tgagccagaa gaaaaagaaa acaaagtaga tgcttcactg
    17281 ccagaaacga ttttaggaga tgtgacattc gatcatgtcg attttgcata tgatccgaag
    17341 aaaccattaa tccaagattt gaattttgaa gtgaaagctg gtcaaaccgt agctgtggtc
    17401 ggtccgaccg gtgccggcaa gacgacattg atcaatctat tgatgcgttt ttatgacgta
    17461 gacaaaggag caataaggat tgatggaatc gacaccaaat caatgagtcg cagtgatgtg
    17521 cgttcgctat ttggtatggt cttgcaagat gcatggcttt atcaaggaac gattgcagat
    17581 aatatccgct tcggaaagct tgatgcgaca gattatgaag tagttgatgc tgcgaaaaca
    17641 gcaaacgtag atcattttat ccgaacgatg ccagatggat atgatatggc catcaattct
    17701 gagggagaca atgtgtcttt aggacaaaaa cagttgctga cgattgcccg agcagtggtc
    17761 tccgatccta aaatcttgat tttagatgaa gcaacaagtt cagtagacac ccgtttagaa
    17821 gcgttgattc aaaaagcaat ggatcgcgtg atggagggac ggacaagttt cgtcatcgct
    17881 caccgtttat cgacaatccg tgatgcagat ctgattctag tgatggatca aggtcagatc
    17941 attgaaaaag gaacgcatga gagtttattg gcacaaggtg gattttatga aaaactgtat
    18001 aacagccaat ttgctgagga agcagaataa gtcatataag aataaaacag tttaaaactt
    18061 attttttgtt ctctattctt gataatggta aagtaaagtt atcaagaaaa gtgaggacac
    18121 ggagatggaa cagtttttaa atgaaaaaat tactgaagcc aaaagagaag ttgatcgttt
    18181 aacgcacgtc tgtcagacga agccttttga ttcagctgat ttttttgaaa atgagtttca
    18241 aatcgaacgc ttggcagcac aaatagaagt gtacgaatta attttaaaaa aagtaataga
    18301 agaaaatata gatgtacaat aaaaacaggt aaaacgtcag ttaccagata ctgtaaatag
    18361 acatctggcc tgagcaggtt ttacctgttt tttttcttat gaaataaata tatgctatga
    18421 tacctttatt caataggatt agtttctaca aaaaatgaaa ggagaaaaat aaatatggaa
    18481 cggtttatag catcaaatgc cacagtgatt ggtgatgtga cattgtcaga agatgtgacg
    18541 atctggtatc aagctgtttt acgcggggac agtaattgga taaaaatagg acaaagaacg
    18601 aatatccaag atggtacaat catccatgtc gatcatgatg cacctgtaga tattgcggag
    18661 aatgtgacgg taggacatca gtgcatgcta catggctgta caatcgaaaa aggggcgctg
    18721 atcgggatgg ggaccacgat attgaatcat gcggttattg gtgagaatag tttgattggt
    18781 gctggatcat tagtaacaga aggaaaagtt attcctccta atgtcttggc ttttggtcgc
    18841 ccagctaaag tgatccgtcc tttgacaaaa gaagaaatac aaaaaaacaa agaaaacatc
    18901 cagcattatg tcgagatagg tcggatgcat gcgcaaggaa aattcaagga gcaaagatag
    18961 gcagtagcat ccataaaata aaaaaggatg gtcagtttga gttgaccatc cttttttatt
    19021 tggtttagat aagagagctt aaaaaagctt cctttgaaac agattaattt aatgaagaac
    19081 tgttttccat gacttcatcg atcaatccat atgcttttgc ttgttcagct gtcatatagt
    19141 tgtcacgatc tgtatctttt tcaatcactt caagcggttg tccagtgcgt tcagctaaaa
    19201 ttttgtttaa gcgttcgcgt gtttgcaaaa tatggcgtgc cgcaatttcg atttcagttg
    19261 cttgcccttg agcgccgcca agaggttgat ggatcatgat ctcagcattt ggtaaagcaa
    19321 atcgtttacc ttttgtacca gctgtcagca agaagcttcc cattgaagca gccataccca
    19381 taacaattgt ttggacatct gctttgacaa agttcattgt gtcgtaaata gccatacctg
    19441 ctgtaactga accaccagga gaattgatat aaagatagat atctttttca gaatcttgag
    19501 cgtctaagaa aagcaattgg gcgatgatcg aattagcaag atcatctgtt acttggccgc
    19561 ttaacatgat gatacggtct ttcaataaac gtgagtagat atcgtaagct ctttcacctc
    19621 gtgaagattg ttcaatgact gtaggaatta aattcataat agagttcctc cttgatttcg
    19681 tttagaaata gtgtaccata atttagtttc taatacctaa aatgtagtat acatttttgg
    19741 tcaaaaaagg tcaaataaaa aacttaggca aattagtcgc ataaacccgc tttatggtat
    19801 gataagcaga gaaacaaaac aaggaggaac aacagattga tagttggtat tactggcctt
    19861 atcggagtgc tgacaggtgc tgtagttgtc tatatcggca tgatactgca aatgcgtcac
    19921 ttggaaaata aagaacagcg tagacgtgaa ttggaattga aagtcaaaga aatcgagacg
    19981 ctgaatcaat taaataaaaa gatcaacgag atcttgcaaa aacggacaac gctgatggaa
    20041 gaatatgtca gctttgatgc atttgatgat tgttatatca cgattgacga ttttgcgtat
    20101 ctccaatcat tttcagcgca aaatagtttt tatctgccaa attatatttt agacgagcta
    20161 tttaagaata tctcccatcg aaaagtgatt ttatctccag atgaaacaat gaagattggt
    20221 ggatatgctt ataaaggcgg acgcgtgatt ttagagaatt ttacggatag tctgactgaa
    20281 atgatcaatg aaaaaaaagc tcagttgaag actatgacgc gccagccgat tggtttcttc
    20341 tcgaaagatg atgtttggag ctgatatttt gaagcattgc cgttgcacat gagagcgtgg
    20401 taatgctttc tttttttctt aatgatacaa tagaactgaa cggagtgaag aaagatgtta
    20461 caaatctatg cccatcgcgg aagcagcggc acccacccag aaaatacgct tcctgcattt
    20521 gcagaagctg tacgagtagg tgcagatggt atcgaactag atgtccatct aagcaaagat
    20581 ggttatttga tcgttatgca cgacgaagaa gtggaccgga cgactaacgg taaagggctg
    20641 attcgagaaa aaacattgga agaactcaaa aaattgaatg cagggggcaa attcagcaaa
    20701 gaatttccag cagctaaagt tcctgaattg agagaagtgt taggactgct cctccaaaaa
    20761 aattaccgag ggatgctaat gattgaaatt aaaactgatc agtatgaata tcccggtatc
    20821 gaagaaaaat tagccgaaac attaaaaagt agagattggc cttttcaaca tgcatattgc
    20881 agctttcatt tggattcttt ggaaaaaatc gcattactgg aaccggagat acagctggac
    20941 ttattgatga gtacttctga taaaaagatc caactagcag cagaaacccc ttatatagaa
    21001 ggagtacatc caaaaaatac ttggttgttc gaacatccag tccaggcagc tgattttcca
    21061 aaagcaattc gtgcatggac gattaacgat gaagaagaaa tggccaaatg tcaagaactc
    21121 aaaatagatg gaataataac tgattttccg gagaaagctc ttcggtggaa acaatcaacg
    21181 aaaagtaggt aaaatatgga aaaagtatta gtgatcgtcg gtccgacagc agtcggtaaa
    21241 acagcgctaa gtgtggaact cgcacaacta tttggaggag agatcatcag tggtgattca
    21301 ttgcaaatct ataaaaatct ggatatcggt acagcaaaaa tcaaagaaga agagatgaat
    21361 aatgttccgc atcatttgat tgatgtgatc gaacctactc aacgttactc agccgctgat
    21421 tttcagcatt caggtcgaca attgattacc gagattgcga accgagggca cctacctatc
    21481 atagctggtg gtacgggtct ctatatccaa tcccttctat atgattatca gttaggagcg
    21541 acagaagaag cgaatcctga gattagaaaa aagtatgaaa cgttagctga aacaattggt
    21601 tcccaagcat tatggaatca gttgaaagaa aaagacccgg cagctgctga gaaaatccac
    21661 tggaacaatc aacgcaaagt gataagagct ctggaagttt tagagacgac aggacatagt
    21721 atcacggcac ctaaagaaga gccaaagcaa ttgtatgatt acctcatgat tggattagat
    21781 acgaagcgta gtattcttta tgaacgaatc aatcaaagag tcgatttgat gctagcagaa
    21841 ggtttagtag cagaagcgcg taaggtgtat gagcttggag aagtccaggc cagtcaaggt
    21901 attggatata aagaattttt cccttatttt gaagggaaag aaacattgga agaagcaaca
    21961 gaacaagtca aactacattc gagaagatac gcgaaacgtc agttgacgtg gtttcgtaac
    22021 cgtttggatg cacagtggtt tgatcttata agtgagccaa atcaaaaaag tcagattgag
    22081 caaacaatca aaaaatggct ggaggcggaa taaaatggaa gaaaaagtga tcatcgtagg
    22141 ggtagaaacc gaagcaaacc agcgttactt ttcagagtca atggaagagc tagttcagct
    22201 gacgaatacc gcatcaggag aagtcgtatt cacaattaca caaaagagac cacaagtgga
    22261 tcggcaaacg attatcggta aagggaaatt acaagaatta gttcaacaag cggatgcgca
    22321 tgaagcggat ttgatcatct ttaatcatga gctaactccg agacagagcc aactgattac
    22381 cgatgctgtt ggtcttccgg taattgatcg cgtgcagctt atcttagata tttttgcgat
    22441 gcgtgctcgt tcgaaagaag ggaagctgca ggttgaactt gctcagttag aatacctgct
    22501 gcctcgttta gtagggcaag ggaaaacatt atctcgtctt ggcggtggga tcggaacaag
    22561 agggccaggt gaaacaaagt tagaaacaga ccgccgacat atacggaata aaatttttgc
    22621 tgttaagaaa gaattgaaag aagtagaagc tcatcgagag cgcaatcgcc aaaaaagaaa
    22681 aaatagcgag atttttcaga ttggacttat tggctacacg aatgccggga agtcaactat
    22741 cttgaatcta ttgacacaag cggatactta ttcgaaagat caattgtttg caacgttaga
    22801 tccattgact aagagatggc gttttgctga aggatttgag attactgtaa cggataccgt
    22861 cgggttcatc caagacctgc ctacgcaact gatcgatgct ttccattcta cattagaaga
    22921 aagtcaaaat atggatcttt tgctacatgt agtcgatgct agttctccag atcgtatttt
    22981 acaggagcag actgtcttaa aattgatgga tgaactgaat atgaaagaaa tgccgatact
    23041 gactgtttac aataaggcag accagatcga cccagcgatg ttcacgccta cgctattccc
    23101 aaatgtattg atttctactc agacgaaaga aggtaagatg gcgttgatcg aagcaatcaa
    23161 acgtcagctg atggaactaa tgacccctta tacactttct gtgccaagca acgaaggaca
    23221 gaaattaagc gagttgagaa gacaaacaat ggttttggac gaagcgtaca tggaagaaac
    23281 aaacgaatat gaagttaaag ggtttgcaaa aaccaattca aaatggttaa gaaaaaacca
    23341 ataaaatttt atttttttaa
//
LOCUS       DACIWG010000040        22863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.040, whole genome shotgun sequence.
ACCESSION   DACIWG010000040 DACIWG010000000
VERSION     DACIWG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.040"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(153..551)
                     /locus_tag="IYF54_001800"
     CDS             complement(153..551)
                     /locus_tag="IYF54_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ9832130.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IYF54_001801"
     CDS             complement(548..1321)
                     /locus_tag="IYF54_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ9832131.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IYF54_001802"
     CDS             complement(1318..2001)
                     /locus_tag="IYF54_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ9832132.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IYF54_001803"
     CDS             complement(2168..3055)
                     /locus_tag="IYF54_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9832133.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IYF54_001804"
     CDS             complement(3052..3921)
                     /locus_tag="IYF54_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9832134.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IYF54_001805"
     CDS             complement(4058..5344)
                     /locus_tag="IYF54_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9832135.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IYF54_001806"
     CDS             5617..6195
                     /locus_tag="IYF54_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9832136.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IYF54_001807"
     CDS             6195..7124
                     /locus_tag="IYF54_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832137.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IYF54_001808"
     CDS             7121..8857
                     /locus_tag="IYF54_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9832138.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYF54_001809"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYF54_001809"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ9832139.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IYF54_001810"
     CDS             complement(9753..10262)
                     /locus_tag="IYF54_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ9832140.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYF54_001811"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYF54_001811"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ9832141.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IYF54_001812"
     CDS             complement(11563..12654)
                     /locus_tag="IYF54_001812"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ9832142.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYF54_001813"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYF54_001813"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ9832143.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYF54_001814"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYF54_001814"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ9832144.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYF54_001815"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYF54_001815"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ9832145.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IYF54_001816"
     CDS             complement(16617..17591)
                     /locus_tag="IYF54_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ9832146.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IYF54_001817"
     CDS             complement(17572..19455)
                     /locus_tag="IYF54_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ9832147.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IYF54_001818"
     CDS             complement(19473..21698)
                     /locus_tag="IYF54_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ9832148.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IYF54_001819"
     CDS             21813..22847
                     /locus_tag="IYF54_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9832149.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         6563 a         4979 c         4121 g         7200 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaa
//
LOCUS       DACIWG010000041        21799 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.042, whole genome shotgun sequence.
ACCESSION   DACIWG010000041 DACIWG010000000
VERSION     DACIWG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.042"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IYF54_001820"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IYF54_001820"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ9832150.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IYF54_001821"
     CDS             1085..2005
                     /locus_tag="IYF54_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9832151.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IYF54_001822"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IYF54_001822"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ9832152.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IYF54_001823"
     CDS             3311..4789
                     /locus_tag="IYF54_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ9832153.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IYF54_001824"
     CDS             5066..7126
                     /locus_tag="IYF54_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832154.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IYF54_001825"
     CDS             7209..7778
                     /locus_tag="IYF54_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832155.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IYF54_001826"
     CDS             complement(7953..8759)
                     /locus_tag="IYF54_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832156.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IYF54_001827"
     CDS             8897..10411
                     /locus_tag="IYF54_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ9832157.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(10814..11797)
                     /locus_tag="IYF54_001828"
     CDS             complement(10814..11797)
                     /locus_tag="IYF54_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ9832158.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11802..12449)
                     /locus_tag="IYF54_001829"
     CDS             complement(11802..12449)
                     /locus_tag="IYF54_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9832159.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(12534..13166)
                     /locus_tag="IYF54_001830"
     CDS             complement(12534..13166)
                     /locus_tag="IYF54_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9832160.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="IYF54_001831"
     CDS             complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="IYF54_001831"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ9832161.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(13730..14467)
                     /locus_tag="IYF54_001832"
     CDS             complement(13730..14467)
                     /locus_tag="IYF54_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ9832162.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(14464..15453)
                     /locus_tag="IYF54_001833"
     CDS             complement(14464..15453)
                     /locus_tag="IYF54_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ9832163.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15711..16253
                     /locus_tag="IYF54_001834"
     CDS             15711..16253
                     /locus_tag="IYF54_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9832164.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16270..17355
                     /locus_tag="IYF54_001835"
     CDS             16270..17355
                     /locus_tag="IYF54_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832165.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17367..18173
                     /locus_tag="IYF54_001836"
     CDS             17367..18173
                     /locus_tag="IYF54_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832166.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18174..19001
                     /locus_tag="IYF54_001837"
     CDS             18174..19001
                     /locus_tag="IYF54_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832167.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            18998..20071
                     /locus_tag="IYF54_001838"
     CDS             18998..20071
                     /locus_tag="IYF54_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9832168.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            20499..>21799
                     /locus_tag="IYF54_001839"
     CDS             20499..>21799
                     /locus_tag="IYF54_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9832169.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHI"
BASE COUNT         7200 a         3791 c         4377 g         6431 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    10561 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
    10621 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    10681 ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    10741 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    10801 gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt
    10861 ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc
    10921 gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg
    10981 ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa
    11041 tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc
    11101 aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat
    11161 ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt
    11221 gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt
    11281 ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg
    11341 ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc
    11401 caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa
    11461 catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg
    11521 cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat
    11581 cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt
    11641 tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt
    11701 tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat
    11761 ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat
    11821 tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa
    11881 atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt
    11941 tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc
    12001 aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca
    12061 taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca
    12121 taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt
    12181 ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa
    12241 atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca
    12301 aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc
    12361 cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt
    12421 ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc
    12481 attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta
    12541 gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga
    12601 cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa
    12661 acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc
    12721 atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa
    12781 acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc
    12841 accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct
    12901 gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg
    12961 ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct
    13021 aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata
    13081 gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt
    13141 aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta
    13201 agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa
    13261 tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat
    13321 gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca
    13381 atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca
    13441 atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca
    13501 gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct
    13561 gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg
    13621 cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca
    13681 gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt
    13741 cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt
    13801 ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca
    13861 aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca
    13921 gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct
    13981 gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct
    14041 cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat
    14101 tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa
    14161 aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt
    14221 cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa
    14281 tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg
    14341 tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat
    14401 gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa
    14461 tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag
    14521 tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc
    14581 aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac
    14641 agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag
    14701 tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag
    14761 aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc
    14821 taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag
    14881 actttttagc catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc
    14941 tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac
    15001 aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt
    15061 aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac
    15121 taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag
    15181 cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac
    15241 gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga
    15301 ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac
    15361 ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc
    15421 ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc
    15481 ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa
    15541 ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc
    15601 agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt
    15661 ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg
    15721 gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac
    15781 gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa
    15841 tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc
    15901 aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg
    15961 aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc
    16021 ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg
    16081 aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg
    16141 tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc
    16201 atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg
    16261 ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat
    16321 gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt
    16381 ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct
    16441 acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga
    16501 caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac
    16561 gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa
    16621 gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct
    16681 ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg
    16741 ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa
    16801 ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa
    16861 gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt
    16921 ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg
    16981 gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag
    17041 caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc
    17101 ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact
    17161 cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg
    17221 atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca
    17281 gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac
    17341 agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt
    17401 atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt
    17461 tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga
    17521 catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc
    17581 tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc
    17641 tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg
    17701 ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga
    17761 aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat
    17821 ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta
    17881 gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat
    17941 taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc
    18001 tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt
    18061 tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta
    18121 tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa
    18181 aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct
    18241 tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta
    18301 cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata
    18361 cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta
    18421 tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca
    18481 tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac
    18541 taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa
    18601 tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg
    18661 ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt
    18721 ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg
    18781 ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg
    18841 caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca
    18901 tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga
    18961 aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt
    19021 tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac
    19081 aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc
    19141 tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga
    19201 ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat
    19261 accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca
    19321 ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc
    19381 agataatcag tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa
    19441 attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga
    19501 cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg
    19561 ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca
    19621 tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga
    19681 agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga
    19741 acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat
    19801 gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc
    19861 ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa
    19921 agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat
    19981 ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct
    20041 attcttagaa ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta
    20101 tcaacgttct aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc
    20161 aaaagcggat gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta
    20221 ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa
    20281 gattgaaaaa gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt
    20341 ctgtaatcta ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa
    20401 acataaaaat catattagat aaaaactaaa ttggtagata aattattttg tcagatatct
    20461 gaggcaaata ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag
    20521 acatattgca gccgatagtt tattatgctt ttagacacat taaactggag gaaaaaatga
    20581 aaaagagcgc taaagatcga gtttttgatg agctgatacg actaacaggg aatatgtctt
    20641 ctgtcactgc cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat gccagccatt
    20701 atttgacgag attagtggaa gataaaaagg tcgagaaatt accgggaaag cctgttttgt
    20761 ggaagccttt agatgaatat gcggttgtag ataatacgga gcaaataaac gaagcctttc
    20821 attctgtagt tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt atagccgctg
    20881 tcaaatatcc gcctaacgga ctcagtgtat taataaatgg agccacgggt gttggaaaaa
    20941 gttttttagc cacaaaaatt tttgaatacg ctatccatga gcaaattatt gaaaaagatg
    21001 caccatttgc tatcttaaac tgtgccgatt atgcggacaa tcccgaactg ctttcggcaa
    21061 cactgtttgg atacaagaag ggggccttta cgggggctga aaaggatacc gatggtttat
    21121 tggcaacggc caacaacggc tatctttttc tcgatgaggt ccatcgccta tcgaaagaaa
    21181 atcaagaaaa gctctttcta tttattgata ctggaaacta tcgtccagtc ggtgaaaatg
    21241 taaattggca ttctgctaag gttcgtttta ttttcgccac gacggaaaaa ggtgaaaatt
    21301 atttattaga cacctttgat cggcgtattc aaattagtgt tatgttgcca acttttgaag
    21361 atcgccccat tcgagaacga ttagagctga ttcaattatt ttttcaaaat gaagcggatg
    21421 ttttacaaaa agatattatt gtttcaagag aagccttgtc tttaatgtta tctcatccct
    21481 ttagtgggaa tattggtaag ctgaagaata tcattaaaat cagttgtgcg gatgtgtata
    21541 gtcgaacaca agaagaacca ttaacgattg ggaaaaatga gatcttgatt cagttaaata
    21601 tgctggaatc cgaagtagaa agcttgccga ttgctagttt attgataaag aaaaatcaaa
    21661 agataaaaac ggatcgaatc aaaccgtttg attatgattc gattttggaa aaaattactc
    21721 agctgataaa tgaaggtaat ttcaatgaaa atttttcttc gataaagaat gaagtacagc
    21781 atttggtgca tcacattga
//
LOCUS       DACIWG010000042        21504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.043, whole genome shotgun sequence.
ACCESSION   DACIWG010000042 DACIWG010000000
VERSION     DACIWG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.043"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(52..1518)
                     /locus_tag="IYF54_001840"
     CDS             complement(52..1518)
                     /locus_tag="IYF54_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ9832170.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="IYF54_001841"
     CDS             1905..2501
                     /locus_tag="IYF54_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9832171.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="IYF54_001842"
     CDS             complement(2559..3041)
                     /locus_tag="IYF54_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832172.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="IYF54_001843"
     CDS             complement(3147..3344)
                     /locus_tag="IYF54_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832173.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="IYF54_001844"
     CDS             3639..4397
                     /locus_tag="IYF54_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ9832174.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="IYF54_001845"
     CDS             4457..4885
                     /locus_tag="IYF54_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832175.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="IYF54_001846"
     tRNA            4931..5003
                     /locus_tag="IYF54_001846"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="IYF54_001847"
     CDS             complement(5153..5524)
                     /locus_tag="IYF54_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9832176.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="IYF54_001848"
     CDS             5739..5924
                     /locus_tag="IYF54_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832177.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="IYF54_001849"
     CDS             complement(6007..6195)
                     /locus_tag="IYF54_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832178.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="IYF54_001850"
     CDS             complement(6219..6482)
                     /locus_tag="IYF54_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832179.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="IYF54_001851"
     CDS             6640..6897
                     /locus_tag="IYF54_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ9832180.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="IYF54_001852"
     CDS             complement(6968..7159)
                     /locus_tag="IYF54_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832181.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="IYF54_001853"
     CDS             complement(7300..7620)
                     /locus_tag="IYF54_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832182.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="IYF54_001854"
     CDS             complement(7643..8077)
                     /locus_tag="IYF54_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9832183.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8412..9050
                     /locus_tag="IYF54_001855"
     CDS             8412..9050
                     /locus_tag="IYF54_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9832184.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9374..9457)
                     /locus_tag="IYF54_001856"
     tRNA            complement(9374..9457)
                     /locus_tag="IYF54_001856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9421..9423),aa:Leu,seq:cag)
     gene            9985..10485
                     /locus_tag="IYF54_001857"
     CDS             9985..10485
                     /locus_tag="IYF54_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832185.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10675..11019)
                     /locus_tag="IYF54_001858"
     CDS             complement(10675..11019)
                     /locus_tag="IYF54_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ9832186.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11173..12093)
                     /locus_tag="IYF54_001859"
     CDS             complement(11173..12093)
                     /locus_tag="IYF54_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9832187.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12280..12771
                     /locus_tag="IYF54_001860"
     CDS             12280..12771
                     /locus_tag="IYF54_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ9832188.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12795..15170)
                     /locus_tag="IYF54_001861"
     CDS             complement(12795..15170)
                     /locus_tag="IYF54_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ9832189.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15496..16122
                     /locus_tag="IYF54_001862"
     CDS             15496..16122
                     /locus_tag="IYF54_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9832190.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16145..16849
                     /locus_tag="IYF54_001863"
     CDS             16145..16849
                     /locus_tag="IYF54_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9832191.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17041..17874
                     /locus_tag="IYF54_001864"
     CDS             17041..17874
                     /locus_tag="IYF54_001864"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ9832192.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            18071..19999
                     /locus_tag="IYF54_001865"
     CDS             18071..19999
                     /locus_tag="IYF54_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ9832193.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            20137..20592
                     /locus_tag="IYF54_001866"
     CDS             20137..20592
                     /locus_tag="IYF54_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ9832194.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            21062..21331
                     /locus_tag="IYF54_001867"
     CDS             21062..21331
                     /locus_tag="IYF54_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832195.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         6684 a         4126 c         3705 g         6989 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctactagaat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacgtgg atttgtcata aattgtcatg cttcttttac ctatgaaata
     8341 ttttcacgtg ttaaagtatt cctcgttgaa cacaacaaat aaaacttttt aacgaataaa
     8401 ggagattttt catgacatca cttaaaactt tactttttgg aacaactttg gctgccggcg
     8461 ctgcattctt catgggaacg actgctcatg cggacgaagc ttataccgtt caatcaggtg
     8521 atactctatc aacgatttct caaaaatacg ttggtgataa ctccttgatc aatgccattg
     8581 cagaatcaaa ttcaatttct gatattaacc tgatctactc aggacaacaa ttaacaatcc
     8641 caacagaagg ctctgcacaa gcagctgctg aacctcaagc agccgttcaa gaagcaccag
     8701 tccaagctga acctgttcaa gctgaacaac ctgttgtaca agaaacggtt caaacggaaa
     8761 cacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa actgctgcta
     8821 ctccagcaag tacaagctct gcaaaagaat ggatcgcaca aaaagaatca agcggttctt
     8881 acacagcaac gaacggacgt tacatcggcc gttaccaatt agattcttct tacttaaatg
     8941 gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact tcacgttatg
     9001 gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa gattcattat
     9061 ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt ctttcgcatg
     9121 acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc agtatttctt
     9181 gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa ctgtcccacc
     9241 ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa tttttcctat
     9301 attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct tctctttata
     9361 aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc tcactagcgc
     9421 ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta gtttaggatt
     9481 acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac gccctccctt
     9541 atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg gttggtgaaa
     9601 aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg ggattagtaa
     9661 ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc tatcttcttt
     9721 catttggata ttgattattt cttctgttga ttactatcct tctggaattc tagacgctga
     9781 agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga acttcaaata
     9841 atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga tatgacacaa
     9901 taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa caaatgaata
     9961 aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc agatttaaag
    10021 aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat caacaatgct
    10081 tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa aacgtcttta
    10141 ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga aagtgatgaa
    10201 catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt aagaaacggc
    10261 gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata tgccacacaa
    10321 ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc acttttgtct
    10381 gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc tgcgatttat
    10441 cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag aaggattcca
    10501 aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat taacttataa
    10561 tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc tagaaaggat
    10621 aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag atttctatcg
    10681 tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg ctggatacca
    10741 gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt catggataga
    10801 ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct taatgactgt
    10861 ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga cgatgtcgat
    10921 tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt tgattagttc
    10981 tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact ccttaacacg
    11041 atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag aagtaagatt
    11101 ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt attctaaaat
    11161 aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata cgccagttgt
    11221 tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat gagttggtaa
    11281 aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat catacatttc
    11341 gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc caccgacaga
    11401 cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta cctcttctgg
    11461 cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg catcaattac
    11521 ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt taaagaagaa
    11581 gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga ctgtttttcc
    11641 agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa agcccatttt
    11701 taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat tcatcaaaat
    11761 attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac atacaacatt
    11821 cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata caaacacagg
    11881 gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc attcatataa
    11941 aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat tggctggaat
    12001 caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa ccactatttt
    12061 ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca cctctcccct
    12121 actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat acttcgaact
    12181 caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg ataagataga
    12241 tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga tgctagcttc
    12301 gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca aacaacaaaa
    12361 gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt agagaacagc
    12421 aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga tgtattattt
    12481 ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga atcagaacac
    12541 gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga tcgtgaaaac
    12601 gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc acaaatccaa
    12661 gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt gattgcttta
    12721 ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta atcaaaaaac
    12781 gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga taaccaaaaa
    12841 cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt ttgccaatgt
    12901 tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa tacaacgatt
    12961 ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt gttcattcca
    13021 ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata tgatttgatc
    13081 agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact gccgatcaat
    13141 cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca aaatccaatc
    13201 aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc ttcctcattc
    13261 atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa tgcttccacc
    13321 gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac ctttccatca
    13381 gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt tcgataagtc
    13441 gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg actcggcagt
    13501 ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag tggtgaaaaa
    13561 ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag aaacaagctt
    13621 ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac tgttgtttcg
    13681 tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa ataataagga
    13741 agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt aattttttcg
    13801 cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg atgataatcc
    13861 caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag tttgttgaaa
    13921 cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt cgttttttca
    13981 taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg tgcttcgtca
    14041 atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa ataaacagtt
    14101 ggatcaaata aataattgta atccccaatg attacgtcac accagatact gacatctaat
    14161 gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac ttcacgagtg
    14221 atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt gtaataacca
    14281 ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca gatcttgtct
    14341 ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc tgtcatagca
    14401 tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt ttctccctct
    14461 tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc tgttccggta
    14521 ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc taccgctagt
    14581 tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa agaggcattg
    14641 cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc agtcagcttt
    14701 ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt gatcacttct
    14761 tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg ttcttgacta
    14821 tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc tggttctaaa
    14881 tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc gtctcttttg
    14941 aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg aactgttgtt
    15001 tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt gcggtggatt
    15061 ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact tccacgtcgc
    15121 aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat ttccaccacc
    15181 tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc tctttccaaa
    15241 cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg gatttttgga
    15301 tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc cttctcttta
    15361 gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag aaataagtac
    15421 ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag ttttggctat
    15481 caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga agaaaactgt
    15541 tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga tgcggaacga
    15601 atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct tacccatact
    15661 cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat cccagtttat
    15721 gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc tggactcggt
    15781 agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt tgaaatgaaa
    15841 acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca ttctatagga
    15901 agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt tagaggaagt
    15961 atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag tattcggacc
    16021 cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga tgcaacgacg
    16081 attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt aggaggagaa
    16141 taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt tcaatacaat
    16201 tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag aaggcattca
    16261 ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta gtgacagtgg
    16321 tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa aagaaattcc
    16381 ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag gtggatatga
    16441 catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg atgaaatgtt
    16501 taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag ataccgctgg
    16561 gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg aagatatcgc
    16621 ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg gtttgacgat
    16681 cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc ttttaaatgg
    16741 cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca tcaatgatac
    16801 gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat ggggatattt
    16861 taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt cttctttttt
    16921 ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa aaacagattg
    16981 ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag gaaggaattt
    17041 atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg aataacggaa
    17101 tgggtgccca tcagtagtac tggtcatttg atcctagcag atgaattcat caaactggat
    17161 gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc gatcttggct
    17221 gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc aacggtagaa
    17281 aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc agccatcatc
    17341 attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt accagttgcc
    17401 attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa caaaaacaaa
    17461 acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct tgttgggtta
    17521 ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat cctaggatct
    17581 atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt agggattcct
    17641 gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg gaattcattc
    17701 aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt ggtttccgtc
    17761 ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc ttttggctgg
    17821 tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat gtagaaaaaa
    17881 ccagaacact tctcttattt gagaaatgtt ctggtttttt attttcttta ataataatca
    17941 ttataaataa taaaaacaac atcaagctta atcaccaaat gataaccatt atcgttctca
    18001 tttagctgtt attattatca attacatttt tattatgcta tccttgtttt gacaaggagg
    18061 tctaataacg atgactaatc agaaaaaagc ttttgcagca acaatcggct gccttttatt
    18121 tatgatcttc ggttttatct tcgaccgatt ctctcttacc atcaccccac ttttttatat
    18181 cagtgccatt atcatcggtg gatataaaca aacatcggaa ggcttgaaag aattgtggaa
    18241 tgatcggaca ctgaatgtgg atttattgat ggctttagct gctattggtg cctgtttgat
    18301 cggtaattac tttgaaggag caatgttgac attcatcttt tgtttaagcg gtgcattaga
    18361 ggaatacaca actaataaaa gtcaaaaaga aattactgct ttgatgaatc ttcaaccaca
    18421 aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa gtagatgttt ctcaactaaa
    18481 ggtggataac ttggtatttg tcgctaaagg agcagctgtt ccgatagatg gctttttaga
    18541 aagcacacag gcaacgatcg atgagtctgc attaagcggt gaatctgtgc ctgtagaaaa
    18601 atatttaggc gatgagatgt atgcaggaac gctgaatcaa ggaaatccca tcacgattcg
    18661 agtaacaaaa acgagttccg aaactgtctt tgctaagatt atccaattag tagaagaagc
    18721 gcagaatacg ccaactcaaa cagcttcatt tatcgaacga atcgaaaata attatgtaaa
    18781 attgattttg cttgctgttc ctctaatgat ccttctcccc cactttttcc taggttggag
    18841 ttgggatgaa agcttttatc gaggaatggt attattagtt gtggcttctc cttgtgcgct
    18901 tgtcgcatct gctacaccag cttctctagc tgctctttca aattctgcaa aaaatgggat
    18961 tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga ttgcctttga
    19021 taaaacagga acgctcacta aaggaaaacc agttgtaact gacagctttt tctttgatga
    19081 aaaggatttg gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc atccacttgc
    19141 tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa gaaatccaat acttaccaat
    19201 agaagaaatt actggttttg gattacaaac tacctattta ggtgcgcaat ggaaagttgg
    19261 caagcacgca tatgatcctg agacgatgat aatcagcaaa gagattgcgg aaatgatcga
    19321 acgtttggaa aatcaaggca aaaccgtgat ttatctttct aaagatcagc agttaattgc
    19381 tgttttaggt ctgctagata ttccaaaagc aaatacccaa caagtaattt cctactttaa
    19441 atcccaaaac atccatacaa gtatgataac cggcgaccat tctggcacag caaaggccat
    19501 tgctgaacaa gtagggattg aacattacta tgcaagctgt actccggaag aaaaaacaca
    19561 actagtcaaa aaagagaatg aactttacca agtaaatgcg atggttggtg atggagtcaa
    19621 tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct atggggcaag ggacagatgc
    19681 cgctatggat atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat ttgtctacag
    19741 tcatcggcta tctttaaaaa tgcggcggat catcaaacaa aatattattt tttcgattct
    19801 tgttattctc ttgttgattg cttctaactt cctgcaattt ttaaatctac ctcttggagt
    19861 tgtaggacac gaaggaagca caatattggt tattctgaat ggcttgcgtt tactaagacc
    19921 tttacctgat ctttctgatt cttcaaaaaa atgtaagtct tgtccactat acaaagcgcg
    19981 gctgtcttct gtcaaataag caaagtgttt ttttatgatt ttgtttattt tatgaaaaaa
    20041 acagcttatg aataggtctt gaaggatgaa ttcctacttt tgtaactttc taaaaacatg
    20101 ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg aaagttggct ttttttagga
    20161 attattttac ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc ggcagctttt
    20221 gttttgatta tgaagctcat accagcctcc cgttcctttt tcccttggct tcaaggcaaa
    20281 ggaatcaata ttggggtgac gatcatcaca gcggctattc taatccctat cgcaacagga
    20341 gaaatcggtc tacgtgatct attagaagcc tttaaatccc ctattggctg ggtagctgtt
    20401 ttttgcggtg ggctagtggc tgtcttatct tccaagggtg taggattgat tgcttctgat
    20461 ccacaaatca ctgttgcact tgttttcggc acgattttag gtgttgtctt cttaagagga
    20521 atcgccgccg gaccggtcat tgcttctggt atcacttact gtatcttaca agtatttgct
    20581 ttaggaaaat agaacaaatt ttgtctattt taggagattc ttaacacccc caatttactg
    20641 atccgtgata tactgacaac gaggtgatac aatgttcgaa aaattaaagt atttcatagg
    20701 aacaatcggc tggtttcttc tagcggtact tttgattctg ggcttagctt atctattatt
    20761 tcctgatttg atacaaaatg tgtttgatac tgctttttga ttgacctatt aaaacgatat
    20821 aggggatgct cctgcgatgc aggggctttt tttaatcaat gttttgtaat aatttaggcc
    20881 gtgccaatga gacttcctat agaattcgat gctctttttt ctttttggct cttctgctct
    20941 tttttgttat actagattac agtggacaaa agaaagaatg atcgcatcca cttcaacgaa
    21001 tatcaaaatg gaagaattag tgcctgtttt caggttactt tgaaaagaaa ggaagacgct
    21061 tgtggaaact tttgaaggtc ttttaattga tacgtacgtt ttacaagaac agccaatgat
    21121 gctgactggg atcaacttgc agcttgagga ccgtgtgatc aatggcctgc ttaaagatcg
    21181 ctatttggca aaatatatct tacgtttgcc cctaaataaa tatgtgctgg aagtaagtgg
    21241 caaatggaac cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa atatcgatga
    21301 atacattaaa ataatcggta caccagaata gcctttactg atcaaaggat actggttata
    21361 aaataactgc agtttcttaa tagaaaaaca aagaacaaca gggaatcgta tcccttttca
    21421 aaaaaaactt tgcaaacggt tctctttgtt atataataac ttagtaccaa acattcccat
    21481 tcaaactttg tttaaataga taaa
//
LOCUS       DACIWG010000043        21128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.044, whole genome shotgun sequence.
ACCESSION   DACIWG010000043 DACIWG010000000
VERSION     DACIWG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.044"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            260..1429
                     /locus_tag="IYF54_001868"
     CDS             260..1429
                     /locus_tag="IYF54_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832196.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1754..3073)
                     /locus_tag="IYF54_001869"
     CDS             complement(1754..3073)
                     /locus_tag="IYF54_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9832197.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(3070..3723)
                     /locus_tag="IYF54_001870"
     CDS             complement(3070..3723)
                     /locus_tag="IYF54_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832198.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            4011..4944
                     /locus_tag="IYF54_001871"
                     /pseudo
     CDS             4011..4944
                     /locus_tag="IYF54_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4852..5568)
                     /locus_tag="IYF54_001872"
     CDS             complement(4852..5568)
                     /locus_tag="IYF54_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ9832199.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(5692..5877)
                     /locus_tag="IYF54_001873"
     CDS             complement(5692..5877)
                     /locus_tag="IYF54_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832200.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            6104..7377
                     /locus_tag="IYF54_001874"
                     /pseudo
     CDS             6104..7377
                     /locus_tag="IYF54_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7484..8515)
                     /locus_tag="IYF54_001875"
     CDS             complement(7484..8515)
                     /locus_tag="IYF54_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832201.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8522..9352)
                     /locus_tag="IYF54_001876"
     CDS             complement(8522..9352)
                     /locus_tag="IYF54_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832202.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(9349..10155)
                     /locus_tag="IYF54_001877"
     CDS             complement(9349..10155)
                     /locus_tag="IYF54_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9832203.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(10161..10985)
                     /locus_tag="IYF54_001878"
     CDS             complement(10161..10985)
                     /locus_tag="IYF54_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ9832204.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(10975..12075)
                     /locus_tag="IYF54_001879"
     CDS             complement(10975..12075)
                     /locus_tag="IYF54_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ9832205.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            12253..13038
                     /locus_tag="IYF54_001880"
     CDS             12253..13038
                     /locus_tag="IYF54_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ9832206.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            13071..13454
                     /locus_tag="IYF54_001881"
     CDS             13071..13454
                     /locus_tag="IYF54_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ9832207.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            13530..13661
                     /locus_tag="IYF54_001882"
     CDS             13530..13661
                     /locus_tag="IYF54_001882"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9832208.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            13630..13866
                     /locus_tag="IYF54_001883"
     CDS             13630..13866
                     /locus_tag="IYF54_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9832209.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(13944..14915)
                     /locus_tag="IYF54_001884"
     CDS             complement(13944..14915)
                     /locus_tag="IYF54_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ9832210.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            15567..17189
                     /locus_tag="IYF54_001885"
     CDS             15567..17189
                     /locus_tag="IYF54_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ9832211.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(17475..18851)
                     /locus_tag="IYF54_001886"
     CDS             complement(17475..18851)
                     /locus_tag="IYF54_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832212.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(18851..19507)
                     /locus_tag="IYF54_001887"
     CDS             complement(18851..19507)
                     /locus_tag="IYF54_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832213.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(19517..20794)
                     /locus_tag="IYF54_001888"
     CDS             complement(19517..20794)
                     /locus_tag="IYF54_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832214.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(20813..20968)
                     /locus_tag="IYF54_001889"
                     /pseudo
     CDS             complement(20813..20968)
                     /locus_tag="IYF54_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT         6591 a         4296 c         3441 g         6800 t
ORIGIN      
        1 aatcaaacat cagccaatcg cttaattgga gccgttctta tggacctgca tgatgaatgg
       61 atttattctt caagaaaata catcaatttt gataagtaga aattgtaaaa acattgtata
      121 gcattttaca caggagtctg gacttgactc aaaaagatag cgtaaaatat tttgtgtaaa
      181 tagaaattta gggtagaaaa aaaagtccat tctgtaaaat taaagttacc acacagaaat
      241 tttataggag gactttctca tgactaaatt tactatagaa attatgcaaa cactactcaa
      301 taaaggggat ttagatgaat tatttcagga tcatttagag cgagcgatta attcacttct
      361 acaagcagag ctaactgctt ttttagatta cgaaaaatat gatcgaaatg gattcaattc
      421 aggaaattcc cgtaacggga attattcacg tacatttaaa acagaatatg gcgagttgaa
      481 tttaactatt cctagagatc ggaatggcga attttctcaa caaacactac cagcatataa
      541 gcgaaccaat gattcattag aaacgaccat tatccaatta tttcaaaaag gcattactat
      601 ggctgaaatc tccaaattaa ttgaaaaaat gtatggtcac cactatacgc cacaaacaat
      661 ctccaacatg agtaaattgg tggctgaaga tgttttagct tttaaagaaa gaacgttaga
      721 agccaattat tccgttatat ttatggatgc gacgcatatc cctgtgaagc gacagactgt
      781 ttcgaaggaa gcagtgtata ttacgattgg tattcgtttg gatggaacca aagaagttct
      841 tgggtttact attgccccaa ctgagtctgc ttatatttgg aaagaagttc ttcaagatct
      901 tagaaaacgt gggttagaag aagttttatt agtagtgaca gacggattaa gctgtattga
      961 agaaagtatc catagtgtgt atccgaatgc ccaatttcaa caatgttgtg tgcatgtatc
     1021 tagaaatatc gctcataaag ttcgtgttcg aggtcgaaaa gaaatttgtg aggatttcaa
     1081 attggtttac caagcgaatt caaaagaaga ggcattggat cacatcgact ttatgactag
     1141 gaaatggaaa aagcagtatc caagagtcgt caatttactc ttgaatcctg ccctattaac
     1201 cttttataat ttccctcacg ccatcagacg aacaatttat tcgacgaacc tgattgaagg
     1261 ctttaataag cagctaaaac gatatactcg aagaaaagaa caattcccta atgaagaatc
     1321 tctagagaga ttcttcattt ctcaatttaa tcaatataac caaaaatttt taggtagaat
     1381 tcacaaggga tttaaagaaa ttcaggatac attagagtcg atgatttaac tgtaattaac
     1441 agaatggatt ttccatttac acataattct tgacgctacc ataatctaaa taaccatcat
     1501 attcatctac tacttatata attgagtttt attaaacttt ttaagtttct attctatcaa
     1561 atatttaaac aatctctgca tccgaaaaga ctacctcgta tagttttact aacaatattt
     1621 ttattagcgt gttcaaatcc tgatttcttg tatcaattat tttctgttca ctttcaataa
     1681 gaaaaaagtc tttacttcca tggataataa taagtctcta tttttcaata actattgcaa
     1741 aattatatct taattaagaa aatattttta ttttaaagat aaaaaattct tcatcctgca
     1801 atactttata ttctatattg taattattca gaatattact tacaatatat aaacctaatc
     1861 cattactatt ttccttattt aaatcaaatt taacatcaaa tattttatcc atattcgaaa
     1921 ttttattgtt accgtatgaa ttctctatat ataaccaatc attaactatc ccaatattaa
     1981 ttaccccatt tacatcagta tattttaccg cattactaat caaattagat agaataatct
     2041 ttaaagctgt ttttcctata taaacattct cgtctaatat ataattattg acagttattt
     2101 tcttttgatt tgctaatatt tcatactttt ttaatatgtc ttctaatgta tcatttattt
     2161 tcaaatattc ttcatcatta ttcaaatttt ctatagaatg aactgataat atttgagaaa
     2221 tattttttgt taaactatca acgatttcaa tacattcatt aatataaata tctctttctt
     2281 tatattttcc aatattgtat ttcatgtttt ccagtattat tttaagacta gcaagagggg
     2341 ttttaagttc atgggatgca cctttaaaaa agtcatattt taacttttct aattttaaaa
     2401 tttctttatt tttaaattcc aaatcgtcaa ttgttcttaa taaagtagaa tacaaatcat
     2461 taatttgttg ttttaattct ccaacttcat cattagagct aacttttaaa ctcatttttt
     2521 tatcaagttt catcatttta tcagtaacaa tttttatttc ttgtatatta ttttttattg
     2581 attttgcata aattaaagaa ataatagcag aaaataaaat tgatattaat aaagaatatg
     2641 gtaaaaattt aaggcttaaa tctttcgcat ctttttgcat atcagctgta gaaacaaatt
     2701 gtagatttgt ttttttacca tcattaagct ttatttctct ttcttcaatt atcaaagaat
     2761 tactatcact ttctaaatta acatttatat tatctttgat ttgtatttca ttcttattat
     2821 ttttttcttt tatgaatact tttatttcac tactcttgga ataaaactcc aaagtttgtt
     2881 ctatatattt tatttctttt ccattcatat tactagaaat ttcatttgcc atattatgaa
     2941 tcttttcttt tctcgtctcc aaatatgttt taggaaagat aaaaaaaatt aatgaatgaa
     3001 ctaatataat tattattcca agaacagaaa atatttgtat gaacattttt ggaaatatct
     3061 ttaatttttt cattttctct ccaatttata tcctacattt cttatagtag ttatacagtc
     3121 taattgtaat ttttttctaa gttcttttat atatacatct attactcgat cataaggagt
     3181 ttcttcgcta tctttccata cataatcaat aatttgcatt cttgttaaca cttgtccatt
     3241 attatccaat aaacatttta atacttctag ttcttttgca tttacatcta tttcttcacc
     3301 atttatttta gctgtatagc tattaaaatt aaccgataga tccttatatt caaacttctc
     3361 taaatgtcca taattcttct taattaaaga atctattcta gcttttaaga ccggcaatga
     3421 aaatggcttt tctacatatc cgtctactaa attagtaaat gcatcaattt tatattcttc
     3481 atcactaaat gcagtcaaga ttagaattgg taaattgctt ttctttctaa tttctttcaa
     3541 cacttctaaa ccatttataa aaggtatctg aatgtctaag ataaccaaat ttatatcgct
     3601 attaaatttt gacaaggctt ctcttccatc tttagcttga ataacagtat atccaaattc
     3661 tgaaagatat tcacttattc cctctcttat catattatca tcttcaacag ttaatatttt
     3721 catatatacc tccacttaag cctatacact agatattata cagcatatta tggctctata
     3781 atatctagtg ttggtgacgc cttctgttgc caatactttt tctttttttt aataaatgca
     3841 aacagacacc ttgaaatcct ttagaaaaaa ggcttctata atattttgtg ttgttggaaa
     3901 atcgaaggcg attttctact aacacttttt cttcgtttca actcaaaaag agactcactt
     3961 ttggtaaaat ttagtcgacc aaaacatcac cctaccaaag gagtcgctct atggataatc
     4021 atactagaaa attactcgga ttaacagata aacatttatt ttttgatgaa gaatggctga
     4081 ttgaaaaaga atataaaggc gttcaagcgc agtttattaa agggagattg gatgactcac
     4141 cttcccattg tgaacactgt ggatcgattc gtattatccg caacggttcc tatacgacac
     4201 gtactcagat gttgaaagtc aaagaaaagt tgactatttt agaattaaaa cgtacccgtt
     4261 ttctatgcta tgattgtggg cagacctttt ctgcgaaaac ggacttagtt gatgaacatc
     4321 atcaactaac aaaggaactg aagcaggcga ttctcataga actgtacgaa aatcaatcac
     4381 gtaaactaat cgctaagaag tacttcgtct cggatgggac ggtgacacgg atcttgcgtg
     4441 aagcaacgaa gcattaccag ccacgtatga acttccttcc gacggtgttg tgtatggatg
     4501 agttcaaatc aatgaagtct gtttctagtt ccatgagttt tatttgtgtt gatgggacaa
     4561 cccatcagct atttacgatc cttgaagatc gcagacttta caaactaact caatacttcc
     4621 tgcactttcc tagaaaagcc cgtttaaagg tcaagtacct agaatgccag ctattgtcag
     4681 ctgcttaaaa cggtctttcc aaatgcggag atcgtaactg atcgctttca tattgtccaa
     4741 catatcaatc gcagcttcaa tcaattgcgt atccagatca tgaatcgttt tcgagcctcg
     4801 cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact cttactcaaa
     4861 gattccacga cattggagcc gctcaaacgg cattatcatc gactgtttaa acgaccaatt
     4921 agtcaaacag aaatcgtcga tgaaccgttg tgcacaatgg tgtcctgaac gtacagcgac
     4981 accttccata tccagagcag tcgccacatc acggggatgg atgccatcca aattaaaagc
     5041 aaccacgcca gtatgatgtt caggatcttg cggtccgtaa atggtgatac cgccgatcgc
     5101 ttgtaattta ggtaaaacgt aggcaaccag ttcttgctca taacgatgaa tattctccat
     5161 accaatctca ttcaagtaat cgattgctcc tgcaatgttc ggagtaccag cttcaaattt
     5221 ccatggcagc tctttccatg aactatcgta aagattgaca aaatctatca tttcaccgcc
     5281 gaattcgact ggctccattt gttctaacaa ttgacgtttg ccatataata caccgattcc
     5341 tgtcggcccg cacattttat gcccgctaaa ggcgaaaaaa tctgcatcca acgcttgtac
     5401 atcaactgtc atatgcggtg ctgcttgggc accgactgga ctatcctcaa ctacaacagc
     5461 ctgttcaacc ctcagccgtt tcaagcacaa caaataacct tctggatctg gtttgccgac
     5521 ctgaagatca tcgatcgtca caaaggaatc aattttttcc ttaatcatcg gatgtgcttc
     5581 aaaaaaatag tctagctccc gcagttcaga attgagacta ctccgatttt ccagtgctgc
     5641 tgatccgctg ctcaaaatgt tcagcatcca ctggtctccc aaccgcttga cttatccctt
     5701 cataaaagga cagttctgct gccgtcccaa caccttgttg atagtctgca aaaggaattt
     5761 tatagccgaa ctgctccagt aaataggctt cagtaaatag tgatgaatac tctcgtaaaa
     5821 catccattca ctgtcaatca tcgtcccatc aaaatcaaaa ataagtgctt tggtcatctt
     5881 ctttccccct tacccaaaac aagcaaaagc tgtgtcctgc tctctctaca gtatcagcat
     5941 aaaaactgcc gtctgaaaag tcaaaggtga aagaaatgtg gcgctataat ttctaggact
     6001 gatagtaaat cattctatca gtccttttga gcataaaaaa acaaaacata cagaagctcc
     6061 gttagaattg aatcaccaca acacaatcaa aggagcgttc tgtatgcctg atcttcatct
     6121 tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg tcgagaagaa
     6181 aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa cgattcagcc
     6241 ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg gaaagaaaaa
     6301 atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt tggcaaagca
     6361 acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca ttgttgaaaa
     6421 aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga ctgaaaaaca
     6481 agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt cgcgaacact
     6541 cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct gttcattggt
     6601 agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt gtatggatgg
     6661 cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc tcatttctta
     6721 ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa cagatatgaa
     6781 cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg ttgtggatcg
     6841 ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat gagtcatgaa
     6901 aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga aggctttatc
     6961 tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa aatggcgtaa
     7021 ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt ctacttctga
     7081 tgaactacga atagcttatg cttactatca agaaattctt caagcttttt atgacaaaga
     7141 agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa gagaactgca
     7201 tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct tagagttgcc
     7261 ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga aacgaatagc
     7321 ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac ttattaaaca
     7381 taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata tcactctatc
     7441 agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt tgttgtgcaa
     7501 aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga atgtataagg
     7561 catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct ttgccctcca
     7621 gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc catagaaccg
     7681 gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt aaataataaa
     7741 tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca taccattcga
     7801 aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc tgatacaatt
     7861 ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc cgatcactga
     7921 ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct agcgattgct
     7981 ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa gcacttaatg
     8041 gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt gccacccgtt
     8101 gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc atatccacca
     8161 atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc tttaccttca
     8221 aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac tgttgaaaaa
     8281 caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg tccagcacaa
     8341 tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta gactttccgc
     8401 acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt tcatcaatag
     8461 ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa aacattttcc
     8521 gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc tgcttaaggg
     8581 acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt cccaccataa
     8641 acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc atccgccgca
     8701 gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc agcatgtact
     8761 ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata cggatgtttg
     8821 gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct gctgcatcca
     8881 ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag acaatcggca
     8941 acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca gtttcagcat
     9001 acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc agcaggagca
     9061 gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata atggtgggaa
     9121 tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag cgacccaccg
     9181 cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta aaatcactag
     9241 gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca ataatcggca
     9301 gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc acgtctgcac
     9361 caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac ctaaagcgac
     9421 tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta aagccgcaat
     9481 tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt cataggtccc
     9541 catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca aaaaaggcaa
     9601 agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg actccagcca
     9661 ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg gaatttggaa
     9721 ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg ctagaaatgg
     9781 caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga atgccagcgg
     9841 aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct gcaatttagt
     9901 cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa tcacactgga
     9961 gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt tgctgaaaat
    10021 atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc tgcacagaac
    10081 cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag gaaccagaac
    10141 caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg cattttctct
    10201 gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg tggtacagtt
    10261 tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc acgatgtgca
    10321 gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt caccacgact
    10381 tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc cgcctgatta
    10441 accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc atcaatattc
    10501 atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg ggcatccgca
    10561 aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg aatcagtcgc
    10621 tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt gtacaactcg
    10681 ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac taattcaaac
    10741 agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt ttcataggct
    10801 ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag ctcacttttt
    10861 accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc aagctgggcg
    10921 acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc aagtctactt
    10981 tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg tcttgttcca
    11041 agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca gtaatttttc
    11101 agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct ttgccaaatt
    11161 gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat tgggatgagg
    11221 ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg ttggcacagt
    11281 tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga agtcccaaac
    11341 aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa cggtggacaa
    11401 acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat ccttttcgtc
    11461 acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg caatcgtcac
    11521 atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag tcggtgcttg
    11581 cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta acaaccaatc
    11641 cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct tataaggtaa
    11701 ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac caatatcggc
    11761 ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg acatatccgt
    11821 atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta cccaatttgc
    11881 ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt tttctataat
    11941 cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg agccgcagcc
    12001 tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt tacgaatttt
    12061 ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac aaagctttgt
    12121 aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg tatatggttt
    12181 gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat agtagaaaaa
    12241 aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc ctttgattca
    12301 gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc tacggatcac
    12361 tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc agcctatata
    12421 aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg tatcgagatt
    12481 ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca tccttatgat
    12541 ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga tgtggtttta
    12601 accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca ggtcttgacg
    12661 gattttccag aaggagatat tttgactcac tttgattatg gattacgccg cttcaccttc
    12721 acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt caaaaaggtt
    12781 gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata cggcaacgaa
    12841 gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct ctttacctta
    12901 ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat gagtcagctg
    12961 ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg ccgcctgacg
    13021 ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa atgagcaacc
    13081 tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat tgggatgcgt
    13141 tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa caactggaaa
    13201 attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt gcagccttta
    13261 aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa gtagccgccc
    13321 cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg gatgaaaccg
    13381 cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc gagtggcgga
    13441 ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct ctctttcaat
    13501 tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt gaaattcgtc
    13561 tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc ctagctgagc
    13621 aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt tattcaaggt
    13681 ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt tttcgctgat
    13741 gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga ccacggcccc
    13801 cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc tgacctgcta
    13861 atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt cgttatactg
    13921 tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct cagccgacaa
    13981 acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc gaaaaaacat
    14041 cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc tgcagctagt
    14101 gagtgtcttt catttatcca taagataaag cttggattca ataaataaat aaatgttttg
    14161 gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc caatgcaaac
    14221 atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc caatgcttgg
    14281 taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa ccgatcaaga
    14341 tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat acctggaacc
    14401 acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg acgaatattc
    14461 ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct ggtaaacatt
    14521 tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc attataagca
    14581 ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt ttgcaaaatt
    14641 tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac tccgccatcc
    14701 gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc agcggtcatg
    14761 gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga acaccaggca
    14821 cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc atcatgtaga
    14881 gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt acacactagt
    14941 ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc tcatataaat
    15001 atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact aatacaggta
    15061 gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat tcccttattc
    15121 gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg aatcaaaaac
    15181 atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa aacggagtca
    15241 gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg taaagtacca
    15301 cagggctgta tattttttat ttcagattac cttaaatgat tccatttact ttttatctca
    15361 ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact atttttggat
    15421 acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg ttatccagac
    15481 tgtagacaaa gtccatttta tggatgatgt ctacagtctt ttcttttata atagatttat
    15541 gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat ggtagaaatc
    15601 aatttgcgat gcttacgatt gatgatctag tttctcaaga tcatctcgtt cgaaagattg
    15661 atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac tccgatttag
    15721 gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag tatttattcg
    15781 gtatccgttc catgcgacaa accattaagg aagtcgatac caatgtcgct taccgctggt
    15841 ttttaggcta ctccttcgag gagaaaatcc cccatttctc aacttttgga aagaattatg
    15901 ttcgccaatt tagagaaacg acggtgtttg aggatatttt tgcctatatt ttagaacaag
    15961 cagttaaagc aggttttgta acagaggata atttgtatct ggattctacc cacatcaaag
    16021 ctaacgccaa taaacataag tttacgaaag aaatgaccca tgatgaagcg aaagcctatc
    16081 aggatgaatt agaagatgag atcaatcgtc aacgaataga ggctggaaaa cgccctttta
    16141 ctttggactt agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa gctgatccgg
    16201 aaagcggcta ctacgtcaaa ggcgaacgag aaaaacaatt tgcttattct gcccatactt
    16261 catgcgacga caatggattt attcttagca caatcatcac tcctggaaat attcatgaca
    16321 gtcaagtcgc ctttcagctc gtcaaacaat cgaagagact ttttcctgaa atcaattgtg
    16381 ttgtcgctga tgctgggtat aagacaccta agtttgttca ctttttgact catttgaacc
    16441 ttcgaccttg tttgccttat tcaaggccca aagggaaaaa ggggctatta tcaaaaaatg
    16501 agtttctcta tgacgagtat tttgattgtt atatttgtcc gcaagatcaa atgctcgctt
    16561 tttcaacggt tactcgagaa aggtaccgag aatacaaatc caatccaaaa gaatgtgtga
    16621 actgtcctct gctaaaccaa tgtaccaaaa gtaaaaatca tcaacgagtc atcacgagac
    16681 atgtttgggg agatctgatg gatgaagtgg aacatcttcg cctaacagat ctgaataaat
    16741 caatttacaa aaaacggaaa caaacgattg aacgaatatt tgcggatgca aaagaaaagc
    16801 atgggatgcg ttggaccaag tatcgaggct tggaaaaagt cgcgacgcat acgatgctag
    16861 tgtttgctgc aatgaattta aagaaattag ccacctggct atggaaaggc aaggagcctt
    16921 tatttttttg ttcaaaaatt cgaaacgaag tagataaaaa gctgttccaa gctcgagtta
    16981 cgagtttgga acagcttttg tctacagtct gcgatgtcgg agacatctcg ttttctatta
    17041 ggaatcaact caggttcaaa agaagcctcc cggtctctgg gaacatcaat gtttacttta
    17101 ccaaagctcg ttttaagcgt cttattgcca taaccgtttc gtcgattgtc atgtttcttt
    17161 ggctctttag aatgggcgtc ataccctaaa tggttattca attctccttg aagcattttt
    17221 tcaaataagg gaccaaacac atcttttaaa acatcttgca tatcctctac agattcgggt
    17281 tgataagtat tcaaaatgga ttcagctaac ttctcagcat caggatttcc tttctttcta
    17341 gccatcgtgt aatcacctct tgatcttatt ttagaaaaat aaaaaccgtg aagcaaccac
    17401 ccgcctagga ttgatggtta cttacacggt ttatattaca ctctcatttt tggtgctaaa
    17461 aagcaccgaa aattttaatt attgtcaatt aataattctt ttggattcct tcttaatata
    17521 ttgaatgaag aaataatcaa tgatacaaga agtactaaac tcataaatat aactacataa
    17581 atcatgaatt taggtaatac attgatatct aaacttgata atgtcttgtt aaatccttca
    17641 gcttctgccc caccacctaa ttggctagat gcagattgtt tagctatttg tttagctata
    17701 tcaccagtaa ccttattcaa tatattattc ccaagcttgt cagctgtata ttgagctaag
    17761 aaataggaac caacgaatgc ggggattgat ataaatacca tctcaattag gaattgacca
    17821 aaaatttcta attttgatat acctaatgat agtaatactg ctatttcttt ctttctagca
    17881 ttcatccata agaataataa taatgaaact acaactcctg caaatattaa tgagccaaaa
    17941 aataatttat ttgaaattga gtaaatacct gatatagatt gttgtaatgc agggtaattt
    18001 gatgaacttt taatcaaatt atattctctc caatttatat ctaatttccc aagatctttt
    18061 attacactat caagattctt atcacctttt acaaagaatg ttgcatcttg gtatacagct
    18121 gtatcttcgg tattaccata aactttagca gctgtatgaa tatcagtgat taatgtattt
    18181 tcgtatagtt cttgtgctgc acttactaca ccgctattat gcccatcgaa tagaccttta
    18241 atttctactt ctactgtttc atctgcacct ttctcattat cagcatcaaa taaattagat
    18301 tttattttta tcttatctcc aactttaagg ttattttttt cagctaaatc tttatgcatt
    18361 aagattttat ttttatcttc attttctaaa tgctttcctt ctactaattt atatgcttct
    18421 gatacaaatt ttgtttcttt tgttgagtcg ttaaccccag ttaacataac tgttctttta
    18481 aaattctttg ctctttcagg tgattgattc gcaagagtct cttgagtttc aataatatca
    18541 taatcaacta aatctgcaac actgtttatt ctttttacat aggagtctat actatctgtt
    18601 tgagatatct ttttaatatc ttcacctttt acatttcccc cacctctagg tgttcctgga
    18661 tttacttgtc tatttatctc catagaaaaa ctatttgtta tattagcaaa tgtttctttt
    18721 gaagctctgt ccgtagcatc tttaatagat aaactaatga gacttaaagt tgacatagat
    18781 aaaataacta atatgataat taatgatttt aaactttttc tagttacata agcaaatgca
    18841 ttttttatca ttattcaacc tccaaattca ttttatttac ttttttcaac tttttaccac
    18901 ttagttctaa aatgatatcc gcagaatctg ctacttcctt actatgtgtt acaactatta
    18961 cacatttatt tctatcttta gctaatgtct ttaatatatt aattatttct ccagcagtaa
    19021 cactgtctag attaccagta ggctcatcag ctagtattat tggagcatct gataccaatg
    19081 ctctagcaat agctactctt tgttgctgtc caccagataa tttcataaca tttcttttta
    19141 tttgtttttt atctaaacct agttcaaaca agatactttc atctgctgat ttatttacta
    19201 atctaatatt ttcaattggt gataaataat ctattaaatt atagttttga aatactaaag
    19261 atatattatt ttttctatga ttactatatc ctttattttg tatatcttca ttcttaaaca
    19321 atatttttcc tgtttgaggt ttatcaagtc cagcaagtaa ggaaagaagt gtagattttc
    19381 ctgttcctga ctttcctact atcgcataaa acttcccaag ttcaaatttt tgatttactc
    19441 ctgacaaaac tttttcttta gaatttgcat aactatatgc tacattcttt atttctaata
    19501 tatccattat tttctcctaa cttatttttg ataagatttc tttaggcttc ttgattaaca
    19561 ttaatgaaga agcaaataca actgataaaa caataatact tattaatatt aaataacttt
    19621 gtccaagtgt tgttatattt gacataaaac tactattatt tattaaactt ccaccggaaa
    19681 tcattgaatc ctctgagtta atgaatccat ctacaattac ttttagtaat acatttccta
    19741 aaaataagga agatattata cttggtatcg atatgaatat taactcgaat ataaattgca
    19801 ttataatttg tatcttagat gttccaatag ataagaatat acctatttca taaattcttt
    19861 cccttaacca tagaatcaag attaatgaaa gaacaaccat cccacctaac ataatagaat
    19921 aagtcattac ttttattata tattttattc cactcactga ctctaaagac tcttcaaacg
    19981 cattagaatc tttttcaaca aaatactttg actcatcaat tttaagctct ttcaatttgt
    20041 ttaaggctaa gtctgtagat tctgcactac cagaatacat taaaatttta tttgcaattt
    20101 cattattttc tgaattattt aatatctctt ggcttgttga ataatctaca aacaccatat
    20161 tttcactaaa atcagaagat aatcctgtat atgtttcctg ttttttacca gaaaagatcc
    20221 ctataatttt aaatttatga ctttttattt ttccactttt ttcaatatct agtaattcaa
    20281 gatcaacttc atcacctaat tttagattgt tttgtttagc aaattcttca tgaacaataa
    20341 ttgaattctt atcattttct tctatatttt taccttcttt aattgtaaat actccactac
    20401 taaataaaat atttctttta gtattatttg tagcttcgag tgaaacaaca ttcttaaatt
    20461 cgtcagataa atcttctcta tttattcttt gttctccgct aactacttta gcatctttta
    20521 attttgctaa tccatcatat tgaattattt tttcttcaat ttcttttaat ttttcaatat
    20581 ctttaaattg attaacatta aaatatttac catctttttt tgttattgat attgaagaat
    20641 tagaactttc atataaagcc ttttctattt cattgcttga tttcattatt gttaaacaag
    20701 aatacaagca agaaagaact atagttaata tgataaaaat tattaaagtt ctatttttct
    20761 ttcttgtaat atatgctata gcatttttta tcactttcct aactactcct ctctattttc
    20821 ttactccata actaattgca tccactggac aagcattttt acatcgtcca catctgatac
    20881 actccagatg attgcaattc tcaactgaat ctttattcat ttggcaaact tttttacatt
    20941 ttccacaatt aatacactta tccttatcct gaatcggcta cactagacta gacagaaaaa
    21001 ataaggtgtg tagaataata aaaaacacac tggaggattt ttcatgccaa gacgtcaacg
    21061 aagaacctat tcaaaagaat tcaaacaaca aatcgtcgat ctctatctcg ctggtaagcc
    21121 tcgcgcag
//
LOCUS       DACIWG010000044        20800 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.046, whole genome shotgun sequence.
ACCESSION   DACIWG010000044 DACIWG010000000
VERSION     DACIWG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.046"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..226)
                     /locus_tag="IYF54_001890"
     CDS             complement(<1..226)
                     /locus_tag="IYF54_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ9832215.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVC"
     gene            complement(506..1120)
                     /locus_tag="IYF54_001891"
     CDS             complement(506..1120)
                     /locus_tag="IYF54_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832216.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            1264..1826
                     /locus_tag="IYF54_001892"
                     /pseudo
     CDS             1264..1826
                     /locus_tag="IYF54_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1937..2911)
                     /gene="lacD"
                     /locus_tag="IYF54_001893"
     CDS             complement(1937..2911)
                     /gene="lacD"
                     /locus_tag="IYF54_001893"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9832217.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2930..3385)
                     /locus_tag="IYF54_001894"
     CDS             complement(2930..3385)
                     /locus_tag="IYF54_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9832218.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(3413..4858)
                     /locus_tag="IYF54_001895"
     CDS             complement(3413..4858)
                     /locus_tag="IYF54_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9832219.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(4855..5790)
                     /gene="pfkB"
                     /locus_tag="IYF54_001896"
     CDS             complement(4855..5790)
                     /gene="pfkB"
                     /locus_tag="IYF54_001896"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ9832220.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5958..6716
                     /locus_tag="IYF54_001897"
     CDS             5958..6716
                     /locus_tag="IYF54_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9832221.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            complement(7211..8410)
                     /locus_tag="IYF54_001898"
     CDS             complement(7211..8410)
                     /locus_tag="IYF54_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9832222.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8853..9344)
                     /locus_tag="IYF54_001899"
     CDS             complement(8853..9344)
                     /locus_tag="IYF54_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832223.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            complement(9371..10516)
                     /gene="dgoD"
                     /locus_tag="IYF54_001900"
     CDS             complement(9371..10516)
                     /gene="dgoD"
                     /locus_tag="IYF54_001900"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ9832224.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            complement(10531..11898)
                     /locus_tag="IYF54_001901"
     CDS             complement(10531..11898)
                     /locus_tag="IYF54_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ9832225.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            complement(11923..12201)
                     /locus_tag="IYF54_001902"
     CDS             complement(11923..12201)
                     /locus_tag="IYF54_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9832226.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            complement(12220..12678)
                     /locus_tag="IYF54_001903"
     CDS             complement(12220..12678)
                     /locus_tag="IYF54_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9832227.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            complement(12690..13310)
                     /locus_tag="IYF54_001904"
     CDS             complement(12690..13310)
                     /locus_tag="IYF54_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ9832228.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            complement(13321..15363)
                     /locus_tag="IYF54_001905"
     CDS             complement(13321..15363)
                     /locus_tag="IYF54_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9832229.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGKDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            complement(15741..16880)
                     /locus_tag="IYF54_001906"
     CDS             complement(15741..16880)
                     /locus_tag="IYF54_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832230.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTLAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            complement(16877..17461)
                     /locus_tag="IYF54_001907"
     CDS             complement(16877..17461)
                     /locus_tag="IYF54_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ9832231.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            complement(17651..20692)
                     /locus_tag="IYF54_001908"
     CDS             complement(17651..20692)
                     /locus_tag="IYF54_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ9832232.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
BASE COUNT         6307 a         4404 c         3449 g         6640 t
ORIGIN      
        1 aacaaaccgc tctatacgtg gctctttggc tagtatagcc attaattatg ccccatataa
       61 cacaagcaat aatcactgta tcgcgttgct tcagttgatc agtatttctt cggtttttaa
      121 tcttatctgg tacacaattg tggtatatgt tagaaacagt tctcataatt tccttgaatg
      181 ttataaaagt atctgtataa tgttcagtat attttgatga acgcatatgt agtcctcatt
      241 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
      301 attttcctct aggaaaaaat taactagcac cacgggtata aatacaaaaa tatttttatt
      361 gttggataca gaaatctatt tttaactggg gatatctttg ccagctaata tctttgagac
      421 tacacctatc ttcatctaaa tggatcaatt actcgctttt tccattgacc ttcgtggtac
      481 ttctgctttc tgatagtaaa aaacattact gaatgatctt actatataac atacagcgga
      541 gatctacttc tttattaagg atcttcatga cgttatcttt ttcttttttc cgttcgaaac
      601 cgcgtcgttc ataaaattga tacgtacagc cgctatccgt gaacagaaat acttcttttc
      661 cttgctctcg gcgttcaaat tcttttaata atttactacc gatccccttg atctttaaat
      721 cagggttggc agctaaaaag acaatttctc catctgggga atgttttttt aaatagtctg
      781 aaagcaattc gtcattggtg tcgtcatacc cgcctttgct gtctttcaca aatgcatttt
      841 gtaagaaatc aaatagttta acatataact tttgactaaa tgaatgatgc ttttttgatt
      901 ttcctttgat gtctgctaat aaaacacctg ccaactcatc atccgcatat aaagcaatga
      961 tttgtgtcgc gcgattcaat tctagatacc aaaagtatct tccataaaga ttcaaaagga
     1021 gtttgttctt ggtataccaa tcaaagtgca tacctttaat cgcgaattga atggcttttt
     1081 tgtagtcttt cttttccagt tgcttaaagg ctaattccat cacttattct cctattcttt
     1141 aaatattgat atgctatact tatattataa tagacaaggt gtcggttatc tatgttcaga
     1201 tagcggtgaa attgtcggat aaccgacaca attccttgat tatgttgatt aggaggctgc
     1261 aaaatgaaaa aagaggatcg ccgcgtcaaa aaaacagaaa aagcgttagc agaagcatta
     1321 tcaaaattat tagtagataa aaaaattcaa aacatcacca tccgtgaact gaccgaaact
     1381 gctgatcttc accgttcgac attttataca cattatacgg atatctatga tctttatgaa
     1441 aaactggaaa atagcttttt ccaagacttg gatgaaatca tttcctacga tacgacacat
     1501 agctacgagg atctatacgc tcaattgatc gactatgtct atacgaatac gttcgtatac
     1561 aagttattcg tcatcaacca cggagatatc caatttcaaa agagattaaa taatctgatc
     1621 gaagaaaatt atttaaaaat ctggctacaa gaagacaaaa aaaaccgtga tcacagaaga
     1681 aatgcgttac ttcaccatct accatatcca agggtgcctc tccgttttaa atcattgggt
     1741 agaaaagcaa ttttgcatac tctaagaaaa aaatgctgac cttcttgcgc ttattgaacg
     1801 acaacataga acctatcatg ccttaaaaac aaacgaaaaa ggaagcgtcg ggacgcttcc
     1861 aattctttat ttaaaagctc aaattgatta ttaaaaatca ttaggttaca aacttaaggt
     1921 tatagttaaa gctttcttac aatttaatag ctgttgctgt ttctttcaaa acattattca
     1981 gctcttcgat gttcttgcgt ccttgtgtct gcatccattc tttggcagca tattcacctt
     2041 tagtaacgaa tggttctaca ccattcgccc aagttgcgcg cccacagagt accccattga
     2101 atgtagagcc agcctctttt gcaaatttca aggtttcttg gaacaattca gcagttactc
     2161 ctgcgctcaa gaaaataaat ggcaggttgg tagtctcgga ttgttctttg aaataagcag
     2221 cagcttcttc tttggtataa gcaacttcat ttttagtgta accatccaca tagttcatat
     2281 taacaggaac ttccactttc aaaacgtcaa ctccatagcg aggcttagaa aattccttca
     2341 tcatttcatt tactttatgc ggttttttca aggcatattc cttcgaagaa gtatcaggat
     2401 cgttggcatc ataagaaacc aattctaaga aaaatggcaa atcttcagca acacattcag
     2461 aaccaatccg ctccataaac gccttctttt ggtcattgat gtcatcagat tcatctacat
     2521 cgtagtataa caagaactta caagcatcgg ccccttgctc ctttagacgt tttacagacc
     2581 aaatagataa aatatccggt aaacgtcctg gagttgaagc atcgtaacca gttttttcat
     2641 aggccaacaa cagaccagca tttttatcgc gaacttcggc tgccggcaac ccatattctg
     2701 gatctaataa gattgctgaa gcatatggag tcaattcaga agaaatgatt tttttgaaat
     2761 tgatgatttg ttcatcggtt gctgatttat tttgaaattt accgatcatt tttttcaagg
     2821 caccacgttg atcgattgct aacgcactga tgatgccact ttcattactt agttgacgaa
     2881 tataagtttt ctttttttct gttaaaggca tgatttcact acttcctttt tagttttctt
     2941 ctaatttttg gtttaaaagt tcagctattt cttcagcaga ctgggcttgt ttcagacctt
     3001 ctgtcacatc tttgtgcatc aaaagtctgg ctacctgaga taaaaatttt agatgggttg
     3061 ttccagcctc tgaatctgga ataaacagag cgattacaaa atccgttcct tctccatcta
     3121 aacttttcca atcgacctta ccgtttaacc gaacaatcac aatatcggca tccttgatac
     3181 tagcatcttt ggcatgaggg atggcaaaac catccatcat accagtcgtg ctttcttctt
     3241 ctctttccat caatttttcg taaactgcat ctcggtcttc actaatgctc aagacaattg
     3301 actgatctgc aaggaagttc attacctcat cttgcgtcgt aaaattttta ttcataaaaa
     3361 ctttatttgt tttgatcaaa gtcatttgaa atccttcttt ctagaataac ctttagttgt
     3421 tggttgcttc tttcatttca acagcagctg cttctgcttc acgtttttct acactacgtt
     3481 tttggatcaa ccctaaaatt actccgccaa cgattgaacc aagtaaaatc gaccctaccc
     3541 atgcaacctt accagtaaca acaggtaata ctagaaaacc tccatgaggt gcgggaactt
     3601 ggacaccaaa catatatgtc agtacagcag caattgatga acctagcatc atagttggca
     3661 gaacacgcag cggatcttta gccgcaaatg gaattgctcc ttcggtaatg tgcgttgagc
     3721 ccaagataaa gttgactaaa ccagcgttgc gatcattcgt gtcgaaatat tttccaaaaa
     3781 acagtgcggc aaatccagta atcaatgggg gcgcgataca agctgccgaa acccctgcca
     3841 taaatgtatg gttaccttgt gccaacaacg cagtccctgt aacataagcc gctttattga
     3901 ctggacctcc catatcaaat gctgacatac agccaacgat aatccctaaa attaatggat
     3961 tagaattttg gaatcctgcc aagaaattca tcattccttg gttgactgct tccattggat
     4021 ttgctaataa agacatgact acaccaacaa acaagacacc gatcaccgga tataagaaga
     4081 tcgccttcag accatccaat tgtttaggca agcctttcat gaccttttgc aatagtaaga
     4141 taaagtagcc tgctaagaaa cctgcaaaaa ttccgcctaa gaaaccggct ccgccagaat
     4201 tggctaacag tccgccaaca aacccaacaa ccaaaccagg acgtttagcg attccttcag
     4261 cgatataagc tgctaaaata ggaaccatca ttcccattgc cgcatcacca acacccttta
     4321 acatagcggc agtcggattg taagtaggat cattgggatc aaaagagtac acaccccaga
     4381 tggcaaacga aattgcaata attaccccac ctgccactac aaacggcagc atatgagaaa
     4441 caccgttcat gagattttta taaatactgt gaccaatgcc tgctgcaacc cccatctccg
     4501 ctttctcacc agcagcgtca actgttttgt tttctgcagt gattgaacct ttaccagcca
     4561 acgcatcttc gatcaattgg tcagcatctt taatcccctt gctgacaggc acatcgatta
     4621 cacgcttacc tgcgaaacgt tctgcttgaa catctttgtc agctgcaatg attacagcgt
     4681 cagcgttttt tatttcttct ggtgttaatt cattctctat ccctacttga ccatgggttt
     4741 caactttgat agatacccct ttgcgcttag ctgcctgctc caatgcttct tgagccatat
     4801 aagtgtgggc aattcctgtt ggacatcctg ttgcggcaac aattttgtaa gttgtcattt
     4861 tttactcgct ccctctttga tttcgatttg tttttccaac tctgcaacat ccgaaaaatc
     4921 tgttaatcct ttacgaaaag cggttgaact tccagctgct aaacttttct tcaaattaat
     4981 atctacctgt tcacctttta aatatcctgc aagaaatgtt cccaacaaag tgtcaccagc
     5041 acacgctgta ttgactactt tacctttagg agaattccca gaataaacga tatccttatt
     5101 taaaaatatt cctccctctc ctcctagaga aaggagaaca tttttcgctc catcggcaat
     5161 caagcgttca gcataatgaa tatatccttc acgatcacta agctcgacat tataccaaga
     5221 ttgcaattcc tcctcgtttg gttttaataa gaacggctga tacggcagac aatcaattat
     5281 ttccggatag cttgaatcaa taatcagatg gaccttattt tcaaaacaaa tacgactgat
     5341 atcaataaga atttcgggtg agacccctcg cggcaaactg ccagaaacac acaaataatc
     5401 gtcagcttgc agcttttgta tttgatttag aaaagttttg atttgttcct gaggaatctc
     5461 aggtccttta ttaaccaatt tgaattcttc attggtttta ttaacttgtg taaaaacgtt
     5521 gattctagtc attcccggta cgctaataaa atcagtgcta atatcttttt gatgcaaata
     5581 atcctcaata taattcccgg taaaacctgc tttaaatcct aaagctgtac tatcgatacc
     5641 taattgcttc atgatcaatg aaacattcac accttttcca ttagcttgta tatcatcatc
     5701 gtttgttcga ttgacttggg atggtttcat ttcatctgtt tcgataaaaa ggtcgattgc
     5761 caagtttaaa gtacatgtat aaactgacat ttacgctcct cctttcttac atattcatta
     5821 tgtatcaaga tcgaatgtaa atgttttcat aaatgactaa attcattgaa aacttttaca
     5881 aaatatatta aataaacttg aagtaaagga aactcccgct ttacaataga attatcctaa
     5941 ctaatcggag gcaaaatatg gatacaaaat taagtgaatc ggagaaatat ctctgggatt
     6001 ttattcaaag tcacatactc gaaatcccct cctattccat tgtaaaacta agtgaatatg
     6061 ccaacgtttc aacagcaacc gtcgttcgta ctatgaagaa aaaaggatat gaggggttta
     6121 cgtcttttaa acattcgtta aaagaaaaag aaaatacaaa tatcaatttt gccgctcttg
     6181 ataaagttga tgaaggaata aagactgcta ttcttaaaaa tgagcaagag gtaatccgca
     6241 ccatcaatat ggtagaaagc gggaatattg aagatgctat tcaacgaatc aaggctgcta
     6301 gaagaatcat gatttttgcc cgaggttttt cagaattgat tgctcaggaa atgatggtca
     6361 aatttcaact taccggtaaa tattgtgaga tgcatactga tcccaatatc ataaaaaaca
     6421 ttagtagaaa attatccaaa gatgacatcg tgatctttgt ttcactaaac ggtgaaacaa
     6481 aggaattgac taccgcagct gaaaactgtt ataaagctga gatcgggaca attttgctta
     6541 ctgctaatcg tcattcaagt ttgatggaac atatcgagat tccttttgtt ggctttaaat
     6601 cagaaggctc cttcttccca gattatgagg tacgctccag acttccactt tctatactgg
     6661 ctagaatctt acttgactcc tatgcactgc gaacatcagg attcacacag cactaactct
     6721 atatctgtaa gactgtacct aaaatatatc tgtgtgaatt aactaaggtt gtctagaaaa
     6781 atatttaatc gctatttgta ttctcgtgct ttctatcatt gctccttcta taacttctta
     6841 gtttcaagat agaatagtgc attgagacta ccaggactac ttacgcaaat atataataat
     6901 aagaatgtga taagcagttc gaagttcctg tctaatattt cttgttaatt attatcaatt
     6961 tccaacaatt ctagaattat aatacgcaaa aaaaggagac cgtctaaccg tctccttata
     7021 gtttattatt cagattacaa tcagttatct aaaatgttac agaagtttta ttagggatat
     7081 tttcttatct ttttagatga ggtttctcaa ttatttagaa atgcttactt aagttattct
     7141 cgttttttgt aattacgcca ataatcacta tatgttattt atataaaact agtatctaag
     7201 tatttttatg ctactcaaca agagaatcac tatcgtttcc tgtcttcggt atttttcgaa
     7261 tgcccaaaat agttaataaa gcacctagca ccgcaaaacc agtaccgaaa atcagcatct
     7321 cccatacacc gaaatgctgc agcagactgc cgcccaagac atttcctatc accccaccaa
     7381 gagttgtggc gatgatgatc atcgtttgtc caaatacacg atcatcatcc gccagaaatt
     7441 gattgaacaa ataagccgaa gctggaataa acaaagcgaa actgaaggct tgtaggagat
     7501 gcgtcatttc catcatcagg atcgaactgg ccagcagcat catgaatcct ctgacagcaa
     7561 atgaaatact ggcaacagac agccaaaatt tgctggattt ctttttgaca agctgttcaa
     7621 aaaagatcat tcccggtaat tcgcataaag cggcgatcat caatgcgatg ccaacttctt
     7681 tactgccgcc gtggatattt tcaaagatct gtgccaaaaa gctgttgatc atggtgtgga
     7741 aagtaaaaag aaaactgaaa cctaaaaata acaaaatcag gaaaggatat ttttttctca
     7801 aagaaactga gcgatcctgt tcttttgtca taggaaaaac ttcttgattt tttacatccg
     7861 gcagcagatg caaaataacg aaaaatatca ctgcttcaac tataaccaag attggcaaaa
     7921 tcgcggcaga ataaatcgct aacagctgtc ccagtaaaaa agaagttaca gcataagaaa
     7981 atgaaccacc agctcttgaa aagccaaagt tgatcctgta gccggtattt gaatactcaa
     8041 atgccagcgc attgagaaat gattgcatag aaactactaa aatcccaccc aagatataag
     8101 taatgatcgg caaattcgga atcaataccc cagctaagag aatcactcct aatcctgcta
     8161 tcatcgtctt cagcacggtg atcagtggga tctttctttt cttgatgatc acttgactga
     8221 caaaaggctg aagcaccgtt gaaacaatat tgaccaatgc taagatccct ccgattttag
     8281 ctggatcaat cttgaaataa atcaaaaaca atgtcgcaaa cccatagaca gaacaaaaca
     8341 tcatccaata aagaaaatgc aaacttgtat aataaaaacg aatcattttt ccgcttgttt
     8401 gatcgcgcac acatctccca ccttattggt ttttctttag caatttacta tagcatactt
     8461 gtaggggttt tactattttt aaaagtggtg aagaactatg gaaattaggg tagattacaa
     8521 tatgcgttga tcaattaaaa catctgttaa taaagacgga aaaaagatca tagaagcatc
     8581 gtactttttt agcatactag cttggtcatt tttttctgga attgaagaaa atatatatat
     8641 tctaaaaact atccatcatc tcttagatta cgagcttttt ttgctgcaaa ggaagttaaa
     8701 aaaagcaggg atcgagtaat cgattcctgc tatatcttgt accaatatat ttctttccaa
     8761 aacttaatat acattgtata cttcaatcaa tatgattcat taaaacaagt ttcattattc
     8821 gtgataaaga aatcataatt tcggtagtag aatcattcat tatctttttt tacaactgct
     8881 cttttttctt ccgaactttg atctaaagta attttggaat ggatatcaag ttcttgaaag
     8941 acatccatca tttcagtttg atctttatca aaaatcattg aaaaagaatt attcttgctt
     9001 ttgtaaaatg aaataaatga tttatcgtca cctagttttt ctacttggac ttgaggatat
     9061 cgattaaagt taccgttcaa tactccaaat ctaacaacag aatcttttgc tagaccctcc
     9121 ttcaaaaaac cattgatcaa aatgacagaa gcaaaagcaa ccagtttaat ctgatcagtc
     9181 agatgttccg gccagaaaat caacaggatg gcgactgtca gtaaaatact caaactcttc
     9241 cttacaggac tactctgagc aaacacgata attttctttt gatcgattat ttgcaaaata
     9301 atcagcagtc ctaaaaaaac tgtgatcata agtgcttctg ccatctttca tcatccttta
     9361 ttttcattta tcaccactca gcgatcgtac cgtcataatt tttccattgc ggattggtcc
     9421 aaatcaaacc ttcttgtgca atttctttga ttttgtcttc atccatttcc aagcccagac
     9481 ctggttttga aggtagatct acgtagccgt ctttatattg gaaaacttct ttgttcttca
     9541 caaaatctaa cagatcaaaa cctttgttat agtggatccc taagctttgt tcttggataa
     9601 agacgtttgg cgtacatgag tctacttgca gagttgctgc tagtgcaaca ggaccatatg
     9661 gcgcatgagg cgcaacagcc atgtcgtaag cttccgccat cgctgcgatt ttacgggttt
     9721 ccaagattcc cccacaaaga gccacatctg gttggataat atcaacagta ccttgtttaa
     9781 agaggttctt gaattgccaa cgagtgtaca agcgttcacc agtagctaat ggtacagcca
     9841 cagaatcagc tacttcttta aagtgttctt cattttcagg taaaacgact tcttccaaaa
     9901 acattggatg gaatggctcc aaagcttttg ctagcacttt tgccatcggt ttatggaccc
     9961 gtccatgaaa atcaacacca atatccattg catcaccaaa acgttcgcga atagacgcaa
    10021 ctcgttccac cacttcatca actttttggt aggaatcgat ataatgcagc tcttctgtcg
    10081 cgttcatctt gacggccgta aagccccgat caaaccgatc ttgtgcttgt tctgcaactt
    10141 cacttggtcg atctccacca atccaagaat aaaccttgat tttatcacgt gctgcaccgc
    10201 ccaataattc ataaaccgga acattaaacg cttttccttt gatatcccac agagccattt
    10261 ctaaacccga aacgatggtt ccattaatag gaccgcctcg gaagaaagat cgatgcattt
    10321 cttggaagag gcgctcgatt tcaaacggat tacgtccaat cagccggttt cctatctcat
    10381 aagctccagc aacaaccgtt tctgttttag ttcctgagat catttctccc cagccttcga
    10441 ttccttcgtc agtcgagatc ttaacaaaaa tccatcttgg ttttacttta tacactgtga
    10501 catctgaaat tttcaattta aaccctccta ctattgtgcc ttcaatggtt catccatttc
    10561 atgattaact tttttgatac catcgaagta ataccatgcg cccgcaaagg ctaataacaa
    10621 cactggaata ccgatgattg gtttgtaagc aaatgcgata aataccaaat aactttggat
    10681 catggaagtc gcagcaaatg atgagattaa cattgaagaa gttcctaagt taataccaac
    10741 cgatttggca attgctgtca gcattggcgc agtagctgac ccacaccata gcaatgaagc
    10801 tgtcacaact aagccaataa tgatattttt taataaatta ccacgagtca aggcaacgac
    10861 cattgccacg cggaaaggaa caaccgctaa atctgcgaat ggtaacacac ggtttcctgg
    10921 caatactaaa gctaatgcaa tcgttagtgg aataatgatc aatgctgttg tgataacatc
    10981 tggattacct acaacaactg cagcatccaa tccgataaat aaacgtgtac ctttgaattt
    11041 tttttgtgac catttttgtg cagcatctga aattggcatc aatccttcca taaataatga
    11101 agtcatctta ggaatcaaaa ccaaaacagc tgacatgctg acacctaatt gtaaaaccgc
    11161 tttgatatca tagccggcta aaacaccaat caccatccca ataactaacc ccatgatcat
    11221 agaatcacca aaaaatccca aatatttttg tgcatctttg atggtgaatt ttgattttct
    11281 gaataacggg atacgatcca gcagccaatt taacggccaa gcaataacca gtgatgaaag
    11341 cgcagaaaca gttggtaaag aaacaccagg gattccgaag aaactttcta ccatcggttg
    11401 cgaccagtct gaaatcttaa aagtcgcaat tgccatcact actgatgcac ctacacctaa
    11461 gaagacattc ttagtcacaa aatacactat aacaccaacg attgccatat ggtgatagtt
    11521 ccagatatca acatccagcg tatgtgtacg tttcaaaatc agcaaaacga tattgaccaa
    11581 tagaatggca aaaatcaaga ttggaatgat tggcgatgcc caagtaacag ccgcaatcga
    11641 accccagcca acatccaaag catctagttt aacaccagtt cgatcaacca tcgccttggc
    11701 agctggtcca acattggtcg tcataaagtc caatatcaac ttgatcccgg caaaaccgat
    11761 acctaatgtc aaacctgatt taaaagcacg agaaatcttt aacccaaaaa tcaaaccaat
    11821 aatcgtgatg atgactggca acataactgt tggtccagca tcaataaaga agttaaagac
    11881 gttcataatg attttcattt tttcagctcc tttgatcagc tattatttca gtaagttcaa
    11941 aatctcttcc attgtctgat cggcaccgat acctgttaaa atcggcatcc ctttgattac
    12001 tggtacacca cctgtttgac catcaaattg accagtcgtc accaatagat catagtcttg
    12061 aacttttcct ggaacttcca tcaattttgt ttgagtcgtt gaaacatcaa tgccatgttg
    12121 ttgcaaatac tctctaactt ttgttgctac tactgttgaa gtggcgatac catttccgca
    12181 tgctactaaa attcgtttca ttatgatttc ctccttatat taaaaaaccg ctttctttaa
    12241 tagttctaat aattcagtat cattgcctgc tctgatcatt tcttgccgca gtggctcatc
    12301 ttgtatgatt ccaacaattt tttgtaacat ttcaacttgc ccatgtggtt ccccaatcgc
    12361 catcatgatc acaatttgga ttggtaactg ctcattgtta tcttccatat tatgaaatag
    12421 gaccggatct ttaagagtcg caattgctaa agttgtttta ttgaccattt catagtttgc
    12481 atggggaatt gcgattcctg gtggtgttga tggtaatcca gtagggtatt ctttttcccg
    12541 ttcttggatt gcttgaatat attcctcctt cacgtagttt tttttgtaca tctcgcttgc
    12601 taaaaacgct aagacttcac tatctgtttt tgcttgaaca tttcgaaaaa cgagttcctc
    12661 attcaaataa agttccaaag tcctcactcc tatttaatag cctcaattaa ttctttcatc
    12721 gaacgtgcta agtttttaac atttagctct tcgatgtctt gcttgttgaa cataccggaa
    12781 ccaagaccta aatagctagc accttgttca aaaaactctt tcgcattctc cttactgatt
    12841 ccaccaaccc ccattagtgg taacttacct aaaggtgctt gaatatcctt gaaaaaacgt
    12901 ggagtaacaa ccgctgcagg aaatactttt acaatatcag ctccttcttt aaacatacgg
    12961 tcgacttctg aaggagtcat tgcggcaggt accgccaata atcctttctt attggcgtaa
    13021 gacaacattt cttttgtgaa catatgtggt cctaataaaa attttgcacc agcctcatag
    13081 caggcttggg cttgttctaa tgtccttacc gtcccagcgc cgatataagt cgtgtcacca
    13141 taacgttgat ttagttcatg tatattttca agagctcctt ttgtgttcat tgtcatctct
    13201 acggcaaatt gatggtcaaa ttcctgcatt gcttgtaaaa tagcatctgc ctgatcaaaa
    13261 gtataaccac gcatgatcac tgtcaattta ggataatctg ctagtctcat gttttcctcc
    13321 ttaagatctt tgtcttaata gttgataaac tgtcttttcg tcatcagcaa tcaataactg
    13381 ctcaatcaat gaatcatctt tggcgatatc agcgatttga ttgatggctc gcagatgctt
    13441 cgttaaatca aaaaatgaaa gcggcaaaat caagctgaca tctttaccat taggaaatcg
    13501 tatcggcttt ttacaaacta aaatgctgac accgtcactg attacacctt tatcgaccgt
    13561 tgtatgggga atgcaagtct tcattcccaa gtaacctgca tatcccggtt gtctgatttg
    13621 atccaagcaa tcagcgataa aacgcgagtc aatgatttga tgttccaata acggtcggca
    13681 agccacctcc actgctcgtt cccaagacat ctcttcttca acgatttgta cataactcgg
    13741 ccgcaagtaa taagtcaatg aaggcaatac attgaagttg tccagcggcg tgcttccatc
    13801 ttccttgatc aaaaagtact gcagttcttc tcgcaacgaa actggattgg tgatatttgc
    13861 atatttccta atgattgtta acaattcatc cattgctcga tcaaccttag taattcctaa
    13921 atcactcaaa actcgatatc gtaaactgat ttgttgctga tatgtcatga ttggctcgat
    13981 cataaactgc agcatagaac tttttagtgg cgtcgtagta aagaccagat cataatcagc
    14041 ttcaaatttg tagaaatcac gcacagatag ggaagctaaa aaatggatct ctggaaaaag
    14101 tttttccata ttggctcgta ctaactttga gaccatgaca ccattagtac aaacaactac
    14161 agccctttgt cgttgcttat tcaatttatt atgattactt aattgatagc caaagtaata
    14221 ggataaaaga tctaattcat catttggaaa ggccttattt agccaattct caataggaat
    14281 aatcaattct ttcatcaaat caatcaaaat cgcatgatta ctatccttga tcagactatc
    14341 tattgaataa actcctaaac tcaagttata gcgtattcga aaacaggctg gacgcagatg
    14401 actcagtatc ctacgttcaa agtcttctcg gtcatcgatc aaaatcaacg tttgattttg
    14461 gaaattttca accatttggc tgatcaattt tcgcaattct tgatccgaat cataattttg
    14521 agtagttttg ctctcaaaaa tattggaagt taaaaataat aacgtgaacc actccatata
    14581 acttttggat agactccaac ctgtttcatg taccaagatc atcagcattc ggaattcagg
    14641 agtatccgtc acatcatcgg gaaaaaacgc gtgcttatct gacatcttga tcccacggct
    14701 tactaaaaat ctcgctgatg tctgcaaata gtctaccgac tcatcagaat aactaagatg
    14761 caacatttgt tccatgttat ggatcaaatg gacgatttct tcttcagaaa catcttgtgc
    14821 caattttctg cgaatcattt ctcggcgaaa aatcggaaac cgttttacta gatccgataa
    14881 cagctgtaag atacggtgtt ccgaaccaac caattggtaa ccattcatcc gattatatcg
    14941 aattgtcaga ctataatttc tgatgaacgg ttccgttcgt ttgatatctt ccataacagt
    15001 cgtcttacta acatcaaccg catctaccaa atgatccaga ctgatgtaat cgatattagc
    15061 gatcagagtc atcactatca atgcttgtct ttcactatca gtgagaacga caagttcctg
    15121 atcatcacct atgtcgtaat caggctgtga tatcatttgg ataacctcga ttggtaccgt
    15181 aatatttcca gaatgacttc tctgaattaa cggaatgttc tttgttttca attcttcatt
    15241 aaattgattg atcgcgtaat tgatttgacg acgtgtcaaa tcaagttcac ttgttaaagt
    15301 ggaaagtttc atttcagggt ttttaataat cagtttgagt atttcacgaa ttcgtttatt
    15361 cattttgact atcctcctga caaaatcatt atatacttgt tcacatacgt ttccggaaag
    15421 ctttcatcac gttttttgtc acacttggtt taatgttttg tcacactaac agctattttt
    15481 aaaatgaacg ctgttatatc aaatgtaaca tggtatgaca attattttag ggctaatgtg
    15541 aatattaaat attcggtaga atattggatg ctttagtaaa taaacacggg acgttgcttc
    15601 ttcaacacat taaagtatct attccgtaac taaatcaagt cagtctaatt gaaaaagaat
    15661 aatattaaac tgctaaaaac aatgcttttg ggagcgtcaa taattttgtg taaatggttc
    15721 ttccctactg tagattgttt ctaattctcc atcggttgac tcagcatttg ttcgagctcg
    15781 ccttcggcct gttggaaacc tttatgactg cgacctagga atttttggtt gtaagtatca
    15841 aaagacgaga ctaagaaacg ttccatcgac tcttcatttt ggaactgttc cttgcggtgg
    15901 ctgtatttct tgatttgctt attaaaggat tcaatcaagt tcgtagagta aatagtcttg
    15961 cgtatggcta atgggaaatc atagaaagtg agcaggtaat cgttcgaaag gatcgattcc
    16021 accatttttg gataactggt tttccatttt tccgcaaaag caccacgtgc ttccaatgcc
    16081 gcctttttag atgacgcttg atacaccatt ttaaaatcat cacagacttc cttacgatca
    16141 tcgacacgca ctttgtgact gatattacgg gaaacgtgca cacagcaatg ttgaaaacgg
    16201 gctttggggt agaaccgctg cacggcccct gccatcccct ttaagccatc cgtgatgaaa
    16261 aggaggacgt ttttcacgcc acgttcctgg aggtctatta aaatctcttc ccaaatcgtg
    16321 atagattcag ttggtgcaat cgcatagctc aacacttcct ttgaaccgtc cgggcgaatg
    16381 ccaactacga tatgaatggc ttccttggcg agggttttcc gctttaaagg gatataggtt
    16441 gcgtccatat aaatagcggc ataacggtca tgaagctcac gttccttaaa tgaagtgact
    16501 tcttccgtaa atgatttagt catattggac atggtttggg gcgtgtagtg atgcccatac
    16561 attttctcaa tcaagtctgc gatttccgac atggtaatac cttttcggaa gaggtgaatg
    16621 acggtctcct ctaaggtatc attcgtccgt ctataagcag gaacagtctg ttgcttgaac
    16681 tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc cgtactcggt cttgaccgta
    16741 cggtcatagg agccgttacg agaattaccc gtgttaaaac caatgcgatc gtacttttcg
    16801 taatcgagga aagccgttaa ctccgttttg agaagcgtat tgacagcttt ttccaaatgg
    16861 acacggaata attcattcaa atcgcctttt tactgataaa acgataaaat gatagtggtt
    16921 cgtttgttgc atactcattt aatagatgtt gaattaaaat atagttcttc gaataattac
    16981 tttttgattt aacatctgct tcaccactta ctagaatact agcaaattct tcattcccct
    17041 catcccccat tactcgagac ataatcagaa ttttatctgg tttaacctca cctgttaaat
    17101 catcttgttc ccataacgcg ggttcaattt ggcttttcaa gaataaagct tccttttcac
    17161 aactattttc taatttagca tagatagctc tcaaaaataa aaatagcgta gttatccttt
    17221 gctgtccatc aatgatttct tgctcgttat gatcattttc ataagccaca atgcttccta
    17281 aaaagtaagt actctcgtta ttgtttgctg tgtactccgc taaatcatca aatagtactt
    17341 ggttttgctc gtcagtccag gcataaggac gttgatattc tggaataacg aattttttga
    17401 ttttccctgt ttcaagaaac tgcttcacgc tttgtttgtt cacttcaatt gtcgttgaca
    17461 tagtttaaat ctccgagacg acactgttta taaaaataaa tctatttcct ttattctgac
    17521 atagctgata tgacagagtt cagacaattt agtacgtaac aaatttttca gggctttctt
    17581 aaaagcaaac aatacaactt ctcctttatt tttgagagaa attgtattgt ctaatcctta
    17641 tttataaatt ttatttcgtc cgtaataact tgtataaatc tctagcaact aatgtatata
    17701 ctagatcaaa gtacttatca tcatgcccaa acattttctc aaaagctgca ttgttcttct
    17761 cgtactgctc catcacctta tctttaaata catcagggaa cagcgatttt tcaaacatct
    17821 gagcatcgtt attcttagcc atgctgacaa ccttcaaatc tgggttatcg gcaaatgacc
    17881 gatacaaaat ctctactaac acccggtctc cttcgtcaaa atcatttgga aatcgttcat
    17941 tgattttatg aatgatttct tctagtgaat ctcgttcatc cggcggtttg attcctgcat
    18001 caattgactt tggatttact agctcgcctt ctttattcac tagatggatt tcaccggaaa
    18061 agtcctgacg taacttataa tattccaact ttactttgtc agaaatatca atattttctc
    18121 gtgtttcttt aggtagaaac ttcagcaagt atccagtaaa aattcgttcc tccaatagtt
    18181 ccatatcgaa taaccgatca agttgtgaaa tataatcata ccacttgcca aagttacgta
    18241 gagtcacccg aaactggtac tgattatcct tgttcatttc ctcatagcga tccataatcg
    18301 gcttaaacat gctagaaatt cgtccaagca agcgctcatt ctgttgcttt tgggcttgtt
    18361 tcaccaattt catgacacgt tcaatatcac cttgattgta aacattaaat tttcgtagct
    18421 tgctttgagt atcataaatc aagttaacat tgatttcttc tctcaaattt gtttgttcat
    18481 aaaagggttg gaaagcagct tcaatatctt caacatcatt tacaaaatca agaataaagg
    18541 tatccttctt cccaggcatt gttcgattga gtcgtgataa agtctgaaca gctttaacac
    18601 cctttaactt cttatcgaca aacatggtat gcagcaacgg ttcatcaaaa cccgtttgat
    18661 atttttcagc aacaatcaag atattaaaat tatctgtatg gaaagcttcc tttaattgat
    18721 tctcccgaac attctcacca gattttgtga cattcatctt cgtttctgta tattcagtct
    18781 ctccatctac taccgaacca gaaaaagcaa ccaacacatc caagtcatta taacctttgt
    18841 tcgctatata ttttttaaat tctttcatat agcgaacagc gtgtaagcgt gatgcggtaa
    18901 cgaccattgc ttttgcctgc ccaccaatag ctgtacgagt aaccgctcgg aattgctcaa
    18961 ccatcacagc cgtcttttga gtaactgccc aaggatgcaa tgattggaaa cgaacaatcg
    19021 ctcgggttgc ttcactcttt ggtaattctg ggttatcttc aatttcacgg gcaattttgt
    19081 aggaagtact ataggtcata tagttcttca gcacatctaa gataaagcct tcctcaatcg
    19141 cctggcgcat cgagtaaaca tgaaagggtt cgaaatgccc atctggttgt ttcgtaccaa
    19201 acatttccaa tgtcttttct ttcggtgtag cggtaaaagc aaagaaactt aaattttgat
    19261 gtttaccttg tgctaccaat tgttgaacta gttcatcttc agcatctggt aatacatcgt
    19321 caacttcgcc ttccagttca taatactctt ccaaggattt agatttgtcc gaaagagcgg
    19381 cttttagctt ctgggcactg ttcccagttt gtgaagaatg tgcctcatcc acaatcacag
    19441 caaaattacg accttccact tgatctactt cctgatagat cactgggaac ttttgcaaag
    19501 tggtaataat gatcttacgc ccatcattaa tggcttcttt taaatcttga gaggatttgc
    19561 gatcgtcaat cgtttctacc aaacctgtct gatgatcaaa gttcatgata gtattttgca
    19621 actggcgatc caacactgtt cgatcagtca caataataat tgatgaaaaa attgggtagt
    19681 tcagttgatc atgaatagag gctaaatggt atgccagcca tgcaatacta ttagattttc
    19741 cagaaccagc actatgctgg atcaaataat tttctccact accatggctt tttacatctg
    19801 caaccaattt tcgaaccaca tctaactgat gataacgagg aaaaatcaac gttttctttg
    19861 tgacttctct gtcgccttgc ttttcttttt taatctctag attcataaaa cgttggataa
    19921 tatccatcaa attgtctcta gtaagcacct tttcccacaa gtaggcagtt gggtagccat
    19981 ctttattgga cggattgcct gctccaccaa ctcttcccgg cccattggaa ccttgattga
    20041 atggcaagaa gtacgttttt ttaccgtcca actttgtcgt catataaaca tctgtatggt
    20101 ccaccgcaaa atagactaag aagcggcgat taaaacgaaa taccaattct tttggatcac
    20161 gatctttttg aaactggagt ttggcatttt ctactgtttg accagtgtac tggtttttca
    20221 attctaaagc aacgagcgga ataccattta acgacaaaac catatcaatg gttttatggt
    20281 tattaggact gtaagcaaac tggcgagtac aggtaaaact atttgcttca tacttttcca
    20341 tcgtttcatc attcagctct gaagcaggtt tgaagtaagc tactttaaag cgcacaccgc
    20401 ggtctttgat cccattgcgc aatacatgta acatgccgtg ggtttctacc tcttgattaa
    20461 agcgttgaag gaacttgact tctccatcat tgttatagtt tcgttggtga cgtgcccact
    20521 gttttggttg agtcttctgg acaaagccta gcaatttttg acaatccaaa ccaattatgg
    20581 gatcaaagtt tgattcattg aatgtttctt tcttccattt gccatacgta gtcagtgaag
    20641 tctcaatatc ctgctcaaaa cgaatttctt tgttctctac caaatgaatc acctcctagg
    20701 cttctttttt accggttacg tattcgtaga tgagagcttt tttataactc tcaagttcca
    20761 tcaattcagc tatcttatta tcaatcattt gatttattat
//
LOCUS       DACIWG010000045        20401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.047, whole genome shotgun sequence.
ACCESSION   DACIWG010000045 DACIWG010000000
VERSION     DACIWG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.047"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            135..410
                     /locus_tag="IYF54_001909"
     CDS             135..410
                     /locus_tag="IYF54_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ9832233.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            416..1183
                     /locus_tag="IYF54_001910"
     CDS             416..1183
                     /locus_tag="IYF54_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ9832234.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1351..3180
                     /gene="typA"
                     /locus_tag="IYF54_001911"
     CDS             1351..3180
                     /gene="typA"
                     /locus_tag="IYF54_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ9832235.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IYF54_001912"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IYF54_001912"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ9832236.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IYF54_001913"
     CDS             5326..5628
                     /locus_tag="IYF54_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ9832237.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IYF54_001914"
     CDS             5631..6794
                     /locus_tag="IYF54_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9832238.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IYF54_001915"
     CDS             6820..10248
                     /locus_tag="IYF54_001915"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ9832239.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IYF54_001916"
     CDS             10269..11393
                     /locus_tag="IYF54_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832240.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IYF54_001917"
     CDS             11383..11820
                     /locus_tag="IYF54_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ9832241.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IYF54_001918"
     CDS             11804..12142
                     /locus_tag="IYF54_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ9832242.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IYF54_001919"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IYF54_001919"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ9832243.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IYF54_001920"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IYF54_001920"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ9832244.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IYF54_001921"
     CDS             13299..14342
                     /locus_tag="IYF54_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ9832245.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IYF54_001922"
     CDS             14501..15229
                     /locus_tag="IYF54_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ9832246.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IYF54_001923"
     CDS             15345..15590
                     /locus_tag="IYF54_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9832247.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IYF54_001924"
     CDS             15587..15805
                     /locus_tag="IYF54_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ9832248.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IYF54_001925"
     CDS             15941..16480
                     /locus_tag="IYF54_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ9832249.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IYF54_001926"
     CDS             16473..18746
                     /locus_tag="IYF54_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ9832250.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IYF54_001927"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IYF54_001927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ9832251.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IYF54_001928"
     CDS             complement(19919..20173)
                     /locus_tag="IYF54_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ9832252.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT         6837 a         3431 c         4358 g         5775 t
ORIGIN      
        1 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
       61 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
      121 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
      181 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
      241 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
      301 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
      361 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
      421 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
      481 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
      541 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
      601 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
      661 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
      721 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
      781 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
      841 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
      901 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
      961 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
     1021 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
     1081 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
     1141 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
     1201 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
     1261 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
     1321 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
     1381 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
     1441 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
     1501 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
     1561 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
     1621 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
     1681 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
     1741 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
     1801 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
     1861 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
     1921 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
     1981 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
     2041 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
     2101 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
     2161 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
     2221 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
     2281 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
     2341 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
     2401 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
     2461 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
     2521 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
     2581 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
     2641 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
     2701 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
     2761 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
     2821 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
     2881 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
     2941 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
     3001 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
     3061 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
     3121 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
     3181 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
     3241 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
     3301 ccgaaagacg tttaaaagac ttttaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttagaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga gggattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 t
//
LOCUS       DACIWG010000046        19457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.048, whole genome shotgun sequence.
ACCESSION   DACIWG010000046 DACIWG010000000
VERSION     DACIWG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.048"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            133..3660
                     /locus_tag="IYF54_001929"
     CDS             133..3660
                     /locus_tag="IYF54_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9832253.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IYF54_001930"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IYF54_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ9832254.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IYF54_001931"
     CDS             7448..7789
                     /locus_tag="IYF54_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832255.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IYF54_001932"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IYF54_001932"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ9832256.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IYF54_001933"
     CDS             9146..11566
                     /locus_tag="IYF54_001933"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ9832257.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYF54_001934"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYF54_001934"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ9832258.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IYF54_001935"
     CDS             13000..13737
                     /locus_tag="IYF54_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832259.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IYF54_001936"
     CDS             13751..14566
                     /locus_tag="IYF54_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ9832260.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IYF54_001937"
     CDS             14579..15226
                     /locus_tag="IYF54_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9832261.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IYF54_001938"
     CDS             15242..15898
                     /locus_tag="IYF54_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ9832262.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IYF54_001939"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IYF54_001939"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ9832263.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IYF54_001940"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IYF54_001940"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ9832264.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IYF54_001941"
     CDS             18240..18758
                     /locus_tag="IYF54_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ9832265.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IYF54_001942"
     CDS             18865..19365
                     /locus_tag="IYF54_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ9832266.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6540 a         3341 c         4178 g         5398 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DACIWG010000047        19303 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.049, whole genome shotgun sequence.
ACCESSION   DACIWG010000047 DACIWG010000000
VERSION     DACIWG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.049"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            237..1628
                     /locus_tag="IYF54_001943"
     CDS             237..1628
                     /locus_tag="IYF54_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ9832267.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1687..2490
                     /locus_tag="IYF54_001944"
     CDS             1687..2490
                     /locus_tag="IYF54_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9832268.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2523..3377
                     /locus_tag="IYF54_001945"
     CDS             2523..3377
                     /locus_tag="IYF54_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ9832269.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3374..4075
                     /locus_tag="IYF54_001946"
     CDS             3374..4075
                     /locus_tag="IYF54_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ9832270.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4083..5150
                     /locus_tag="IYF54_001947"
     CDS             4083..5150
                     /locus_tag="IYF54_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9832271.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5143..6108
                     /locus_tag="IYF54_001948"
     CDS             5143..6108
                     /locus_tag="IYF54_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9832272.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6153..7127
                     /locus_tag="IYF54_001949"
     CDS             6153..7127
                     /locus_tag="IYF54_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ9832273.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7198..8685
                     /locus_tag="IYF54_001950"
     CDS             7198..8685
                     /locus_tag="IYF54_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ9832274.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            8742..9857
                     /locus_tag="IYF54_001951"
     CDS             8742..9857
                     /locus_tag="IYF54_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ9832275.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9854..11269
                     /locus_tag="IYF54_001952"
     CDS             9854..11269
                     /locus_tag="IYF54_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ9832276.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11302..12249
                     /locus_tag="IYF54_001953"
     CDS             11302..12249
                     /locus_tag="IYF54_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ9832277.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12289..13449)
                     /locus_tag="IYF54_001954"
     CDS             complement(12289..13449)
                     /locus_tag="IYF54_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ9832278.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13614..14789
                     /gene="mltG"
                     /locus_tag="IYF54_001955"
     CDS             13614..14789
                     /gene="mltG"
                     /locus_tag="IYF54_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ9832279.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14925..15404
                     /gene="greA"
                     /locus_tag="IYF54_001956"
     CDS             14925..15404
                     /gene="greA"
                     /locus_tag="IYF54_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ9832280.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15572..16303
                     /locus_tag="IYF54_001957"
     CDS             15572..16303
                     /locus_tag="IYF54_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ9832281.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            16300..17367
                     /locus_tag="IYF54_001958"
     CDS             16300..17367
                     /locus_tag="IYF54_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ9832282.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17374..18006
                     /locus_tag="IYF54_001959"
     CDS             17374..18006
                     /locus_tag="IYF54_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832283.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18146..18811
                     /locus_tag="IYF54_001960"
     CDS             18146..18811
                     /locus_tag="IYF54_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ9832284.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18839..19132
                     /locus_tag="IYF54_001961"
     CDS             18839..19132
                     /locus_tag="IYF54_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ9832285.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19200..>19303)
                     /locus_tag="IYF54_001962"
     CDS             complement(19200..>19303)
                     /locus_tag="IYF54_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9832286.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6741 a         2702 c         3713 g         6147 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaaatattca ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtca tctcaaggac
     1561 tagatgaaga tgaaaatatt agttcattag cagaaatcga cttttcacaa gagaaaattt
     1621 atcgctagat tggaaaaatt ttgagcaaag aggtaggaga gaacattgga aaataaaaat
     1681 gtaagtatgc tgctttcttt atatgataaa gaaaagccag agtatttaaa ggaagcatta
     1741 actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta tgacggccct
     1801 ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat tggattagaa
     1921 catacgaaaa atcgcttagt agccagaatg gatacagatg atataatgga accccaacga
     1981 ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt tgggagtaat
     2041 attgaggaat ttactggcac gcttagcaat gttatcggaa aaagaattgt acctgaatat
     2101 aacaatgcaa ttcgtgtatt ttctaaaaga agaaatccct ttaatcatat gaccgttatg
     2161 tttgataaaa aagctatttt ggaagtggga aattaccaac cattaagtgg gtttgaagat
     2221 tattatcttt gggcaagatt attaaaggca ggttataaag gatataatat tcaagaaaca
     2281 ttagtctatg cgcgaactgg caaagatatg tatgcaagaa gaggcggaat gaaatatctt
     2341 ggttctggtc ttatgggtcg ttatcgtatc tggaaagacg gtttagggtc agtaaaagat
     2401 tttataattg tttcttgcgg acatattttt attagcttac taccaaatca attaagagga
     2461 aaattttatc aaacgcaatt aagaaaatag gaggtttttc tttgtatagc actgatccaa
     2521 aaatgaaaaa aatacaacag attacgttga aaatgtctca agagattgtt gatttttgtc
     2581 taaaaaatca acttctttgt tatttctgcg gtggtggtgc catcggtact gtgagagaac
     2641 aagggtttat tccatgggat gatgatttag atttctttat gcctagagag gattatgaaa
     2701 aattttatca actatgggtt aattcagaaa aaagtaaaaa atatcccata cagaaggctt
     2761 ctaaaaatta taatgatcat aattctttta caactatccg cgatgcagat actacattta
     2821 ttaaaactta tcaaagagat ttagatatag tccatggaat tactattgat attttcccgt
     2881 tagatgttgc ccctgtttcg aaatttagcc gtaaaattca aaaactatgg gctttagtat
     2941 atgctttatt ttgttctcaa gtcgtccctg aaaaacatgg tggaataatg gctacaggta
     3001 gtaagatact cttatctatt ttccgctcac ctcgtacgag atataagata tggtcttttg
     3061 ctgaaaaaca aatgacaaaa tataataatg aaccaacggg ctatgtgact gaactttgtg
     3121 tcggacctaa atacatggga aatgtttatc ctgcaaaaga ctttgaatct gctgtgtggg
     3181 tgccattcga agatacagag atgccagtac cagttggtta tgataattat ttaagtcaag
     3241 tttttggtga ctatatgcag cttccgcctg aaaaagatca ggtttctcat catgaagcag
     3301 tttatattga tccagaacat ccatacacaa tgtacaaagg aaaatattat ttaactaaag
     3361 gagcagaaaa aaaatgatta cagctattat tattgctggt ggtgtgggta aacgaatggg
     3421 acaagatatc ccaaaacagt tcattacaat tgaaggaaaa cctattatta tctatacttt
     3481 agaatcattt caacaccatc ctcaagttga ccggatttta gtcgtttgca aatctggttg
     3541 ggaagaaacg atgtgggcgt atatcaaaga attcaatatt agcaaagtca aatgggtcat
     3601 tacaggtgga tcaaagggac aagagtcgat caataatggt gttcagtttt tgaaagacta
     3661 ttctgaagat gatgatacga tcatcatcca tgatggaatc cgtccactag tagacgaact
     3721 ggtattgtca gatgtaatcg ttaaatgtaa agaatttggt aacggtgtaa cctctctacc
     3781 ttacaacgaa caaattttta tcaagaaaac agaagaaacg acggaacagt atgtcgatcg
     3841 taacacattg aggcgtgtct caactccaca agcttatcaa tacggaaaac tacgggcagc
     3901 atatgaccgg gcagtgaaag aagacatcgg tatgactgat tcttcttata cgaataccat
     3961 gatggtagat ttgggagaaa ctttatattt tgctgctggt tctgataaaa atatcaaatt
     4021 gacaacaaca gatgatctag aattgttcaa agcatattta aaaatgaaag actaggtgaa
     4081 agatgatgac gttgattaaa caaccaatgt accaagaaga tttggcaaaa gtaatcaatc
     4141 atacaccaaa aataaaacag ctacatggaa aatccgttct gatgattggt gcttctggaa
     4201 tgatcggctc ttttttgatt gatacgttga tggctgctaa tgaacaacta ggcatcaaaa
     4261 tcaaagttta tgcaatgggc agaaaccgtc agaagcttga aaaaaggttt gccagttatc
     4321 ttgaattaga tactttcgaa attgttgaag gtgacgtcac agaaccctta ccaaaagaaa
     4381 ttcaagctga ctatttgata cacggtgcaa gtaacacaca tcctaaggct tacgcaactg
     4441 atccaatcgg tacgatcatg actaatcttg caggtacaga gcaagtattg aaacatgcag
     4501 tagcaaccca accggttaga gtattatttt tatcaacagt agaaatctat ggcgaaaatc
     4561 gaggcgatat agaaaaattc acagaagatt actgtggata catcgattgt aatacactga
     4621 gagcaggcta ccctgaagga aaaagagtca gtgaatcatt atgccaagcc tacatcgcaa
     4681 aacacgacct cgacatcgtg attccaaggc tttgccgtac gtttggtccg acgatgcttt
     4741 tatctgatac gaaagcatcc tcacaattta ttttaaatgc agtcgaacaa aaagatattg
     4801 ttttaaaaag tgaaggaaac caatattttt cgtatgtata tgttggagat gctgtttctg
     4861 ctattcttca tttactgctt gaaggagaaa aaggagaggc gtacaacgtt tcttcagaaa
     4921 actttgattt acgactaaaa gaattagccc aacagctagc tgatatcagc ggcaaacaag
     4981 taatttttga attgccggat gagattgaac aaaaaggttt ctcaaaagct agcacagcag
     5041 tacttgaaaa taaaaaaatt aaagagacag gttggaaacc aatttttcct ttgaacgaat
     5101 cattggttca tacagtagaa ttagttaaag gtgagaaata taatgtgtga gattagtatt
     5161 attgttcctg tgtataaagt tgaaaaatat ttaagaaaat gtgtagattc gattttagcc
     5221 caaacattta ctgattttga agttatatta gtagatgatg gatctcctga caatagcggg
     5281 aaaatctgtg atgagtatgc tgaaaaagat aatcgagtac gtgtgatcca taaagaaaac
     5341 ggcggattaa gcagtgctag aaatgcgggg attgatgttg cgagaggcaa atacttaggc
     5401 tttgttgaca gtgatgatta tattgatgaa gatatgtatg aaattttgta tgagaatttg
     5461 aaaattcatg atgccgacat tagctctgtt gaattgattc ctttttatgg tgatagatat
     5521 aagaaagcta ataaagaaaa gaaagtaatt atattaaata aaaaggaagc aataaaatct
     5581 gttttagaag gaacgcaatt ttatgcatac gcatggaata aactttatcg taaggaactt
     5641 tttaaagata atcgttactt ggatggaaaa acatttgagg atgcatatat aataatagat
     5701 ctattatttc aaacagaaaa aattgttgtt tccaatgaag agaaatattt ctatcttcaa
     5761 agaaatgata gtattatggg aaaaagtttt tctatgaata atttcgatgt tatagaagca
     5821 tggcaatata acaaagagaa gattttagat gcttttccag atttgcatga ttcctattat
     5881 cgccgactat gttgggcata tttttacgtg ttggataaaa tggttttatc aagtagttat
     5941 caacaaattc cacgtaaaaa aacagtaatt gaatttttaa agaagaacag aaattttata
     6001 ttaacttata agggattcac taaatcgcgc aaaatagcta ttattgctct gagctttaga
     6061 gtagattggt ataggaaatt ggttgaacaa aaaaataaaa aaaattagaa tgtttaaatt
     6121 agtaaataaa agaaaaggaa ttgaaaacta aaatgtgcag aataagtata atagttccag
     6181 tttataatgt tgaaaaatat ttaaaaaagt gtgtcgattc aattctaggc caaacattta
     6241 ccgattttga attattatta attgatgatg gttctacaga taattcagga agtatatgcg
     6301 atgaacttgc gaaaacagac aaccgaataa aagtgataca taaggaaaat gggggtctaa
     6361 gtgatgccag aaatattggt attgaggtag caaagggaga ttttattggg tttatagata
     6421 gtgatgatta cattgatgaa gatatgtatg catttttata taacaatata ttgaaatatg
     6481 atgcagagtt atcaatgtgt ggtatttatg acgtccataa gaataaagaa attaaaaagt
     6541 taactccttt ttctcaattg gttactaaat ctgaagcaat tgaattagta ttagatggta
     6601 agctagttgt tgctaatgca gtaagcaagc tatataaaaa agaattattt gagaatgtga
     6661 gatatccaaa aggaaaaatt gctgaagacg cagcagtaat actgaaaatt atcaatcagt
     6721 gtaataaaat ccatgttgat acttctcaaa aatattatta ttatcatcgt ggagatagta
     6781 ttactagtaa aaaatttaca aaaaaagatt ttgatataat tgatgtatgg aatgagaatg
     6841 aaaaatggat tattaaaaat tatcctcaaa tgtacgataa agtacatact agagtttgtt
     6901 gggcttactt tgttgtgtta gataaattag ttttaactga agaaaaagga ctgtatcaag
     6961 aaaaaaaaca agtaagagag tatttaattg ataatttctt ttttattata aaaaataaac
     7021 gattaacttt tcaaagaaaa atatctatgt tattgcttac ttttggaatt gctgcgtata
     7081 aaataccggt taaatatcag tataagaaag taaaggctgt taattaatgc tttagttagg
     7141 atgaattaga tgaaaaaaag caggttagag tattctttaa taaattcttc tatatcaatg
     7201 tttatatata ttttaaggat agtaatacaa tttgtaggta gaagtttttt tatttatttc
     7261 ttaggagcga aatatttagg attaaatggt ttatttacaa atatacttag tttcttgtcg
     7321 ttggctgaac taggcatagg ctcttctatt atttattccc tatacaaacc attagcccaa
     7381 aaggatgaaa gtaaagtaaa ggctttaatg aatttgtata agaagacata tgaatttatt
     7441 ggtattttta tcggcatagt aggagtattg attattccgc tgttacctct atttattaaa
     7501 aatagcacag acataccaca tatgtatcga tattatgtat tatttttatt aaattcagtt
     7561 cttagttatt ttttcacgta taaaagatca cttgcaatag cagatcaaaa aaattatctg
     7621 gttgcgatta atgattttgc ctttttattc tttatgaaca ttgtacaagt aattgctttg
     7681 tacttttatt ctaattttac tctatttcta atagtgcaga ttttgtttac tctattttct
     7741 aatataaata tttccagaac agttgacaag agatattcat atttaaaaga gaaagatgta
     7801 gtttctctag ataagcaaac aaaagatgaa gtaaagaaaa atgtcatagg taatatgtcc
     7861 tcaaaaatag gcggggttat tgtaatgggg acagataata tattaatttc tacatttgtt
     7921 aatttaacag ctgtaggaat ttattctaac tattcattga ttgtcacaag tgtgcaaaat
     7981 ttatgtaagc aaattactaa ttctatcacg gcaagtgtag gaaattttgc tgtatcaaat
     8041 aaaaaaaagg attcatttga gctatttaaa aaacattttt ttatcaacca ctcactaatc
     8101 tttttttctt ctatattgtt attgacttta atgaatccct tcattagctt gtgggtagga
     8161 gccaaataca tgctacctat gtcaacgact attttaattg ttttaaatta tagtgtacag
     8221 gtttatagaa atacatcctt cgtttttata gagtcatttg gtttatattg gtctcaacgg
     8281 aaaaagccta taattgaagc aggtgtaaac ttgttattat ctttgctttt gttaatagtt
     8341 tttgatatgg gaatcaatgg agtacttata gggactatcg gatcgtctct aggatttgtg
     8401 atttggtatg aagcatttat catatataaa acggtatttg agaaacgttt tattcatttc
     8461 tttttaactt tctggcgaag ttttatagaa ttaataattt cggcaatagt cgtttttcta
     8521 ttaaatgaat atttagcaaa tggaggcagt acgttccttc aatttataac taaaacctta
     8581 ataactatag taacaacatc gatattatat ttgttattat attttagaag agaagaattt
     8641 acatttatca aaaaaattct ttttaggtat attttaaaaa aatagctttc tataaaggag
     8701 tgttttattt gaaaatagct atactatctc cagctttctc aatgggagga gctagtatcg
     8761 tggcctcaaa aattgcaaaa ggattaatag atagtggcca tgaagttttt tttgtttctt
     8821 acttagaaag tgaaaaacca caagtagaat ctaagtatta tgatttatac gaaaaaaaaa
     8881 tttttttatt taaaaatttt tctcgatttc ataagatatt agagtataga ttgtttggag
     8941 gctttgttcc tagtagatat ataaaaaaag aaaaagaaca actaaaaaaa gttctaaaga
     9001 aagaaaaacc tgatgtcatc atattcaata cttttatacc tagtgtacta ttctccgaat
     9061 tcctaaagta tgaatttcca acagtgaaat taattacttg gatgcactct gatcctgatt
     9121 acagcttaaa tcatattgct aaattttata aaaaagaata tagaaaaagt tttgaacgcg
     9181 tggattccat tgtttgtctt tcgcaagata caaaagaaaa actttcatta tacaataacc
     9241 atattaaagt tatttataac ccattgacat taagcggctc tagtttaata agtaaccttg
     9301 ataaaaaaat tatttcattt actgccagat acgatataaa tattaaaggg tttgattatt
     9361 tatgtgaagt agctaaaaac ttacctgttg gatgggctat aaggattgct ggagatggta
     9421 cagaagaaga acgtaaagag ctgaaaagat taattgagaa aaatggtgtg ttagataaaa
     9481 ttatttgcgc aggtgcatta tttggagatg aattaatcaa tcattatata gaaagttcta
     9541 ttttcatatc tacttctaga agtgagggac taccattagt gttgatagaa gcaatgactt
     9601 atggattacc tgtaataagt tttgatcatg gtggtgctaa ggaaatatta caagatggaa
     9661 attatggaat tttaattaag aattttgacg tacaagatat gaataatgag atagaaaaat
     9721 tgtctaataa tcgtttactt atggaaaaat atcaaaaatt ctctttagaa agagctaagg
     9781 attttaatga aaaaaaaata attggtgaat ggcaaacatt gttaaatgaa ttaggaaatg
     9841 aggatgttag aaattgagga cttcgattaa aaattttaat ttatggaaag aatggtatct
     9901 atatatattt tttataggga tgattattcg tgcaataaat ttttatagtt tattaccgtc
     9961 cacttttgat agtatttttt ttaaacttat aggaatttca ggagttttgc tattagggat
    10021 agatttcata attaaattta ataaaagaaa agtaacttat aacattttat tgatagcata
    10081 tatggtgatt ttagtaataa cgagttttgt tcataaagaa tatggtctag gagaaaactt
    10141 aaagactata atgtgggcaa tcttacagta ttttttgatt tatcagtttg cttttgagaa
    10201 tagcgataat aaacgatttt ttaaaaggat aagttgggga tttatttata gttggtttgt
    10261 tttagtatca atgtcaatgg ttcttttctt tggtaaattc ggctatgaga aatattataa
    10321 tgctagacat aggatcagaa ttggtttttt agaatcaaga ctgtttggga cgttttctga
    10381 tattaacaat gctgctatag catctttagt tgttattgtc ctaatcttat tttatcttta
    10441 taataaaaaa gtgacaggat taaataaaat cattttttta attaatcttc cattacaatt
    10501 tatttatata gtattaagtg ggtcacgatc tacctatgtg gtaagtataa taatagttgc
    10561 catatcatct tttattgttg tgttaaaacg aaatggaaca aaaaaagatt taaaaaacta
    10621 tatccttgct atagtatctt ctattggttc tgttctgatt ttattcgctt tgtttaatgc
    10681 aactaagata ctttttcaag aattattacc tcatttaaaa gaaataaatt tttttaattt
    10741 tacagagcgt cataaagaat taatgaataa tagtcaacat attaataata ctcaaacacc
    10801 tgataatact cttgttagga aagacgtagc tgaaaataca gatatttcta atgctagaat
    10861 tactatatgg aaaagtgcat gggaaatatt taaaacaagt tggcttatcg gagtctctcc
    10921 gaaaaatgta gtatcttatg cacagtcaat tattccagat ggatatattg cgaaagtagg
    10981 aattgccatt cataatgcat acttgaatgt attaacatcg acaggtattc taggaataat
    11041 tcctttcatg gcttttttaa taaagagttt tgtgctgaat gtttatcgaa tcatctataa
    11101 taaatgcatt ggtaaagatt atcattttgt ttactgttta gttattttat cttatgcact
    11161 atacgcagct cttaacaatg aattagtcta tgaaaacact gtaggaacgt ttgttttttg
    11221 gctattttta ggaagagtta atagcaaaaa taattctagc tatttttagt aataaatttt
    11281 atcttgaaaa gaggagaaaa aatgttgaat tacttgatta ccggtggcgc cggcttcatt
    11341 ggctcgacgc tcgcgaatta tctaggaaaa gaaaacaatg tagttgtgat cgatgattta
    11401 tcgatgggga aaaaagagaa tctggatgaa tcgaagcaca tcacgtttat tgaaggggat
    11461 gtcgctgaca aaaatttgat ggaaaaggtc atgaaagaat accatttcga ttatattttc
    11521 catctagctg ctgtagctag tgtggcagat tctgtagaac gtcctctaga aactcatcgt
    11581 gtaaattttg acagtgcgct tttattgctt gaattggtac gaaaatatca atctggttta
    11641 aaacgattgg tatttagttc atcagctgca gtttatggcg atgaaccaac gttgccaaaa
    11701 aaagaagaat cggtcatccg tcctttaaca ccatatgcca tcgataagtt tgcagcagaa
    11761 caatatgttt tggattactg tcatttatat gatgttccga ctagtgctgt ccgatttttt
    11821 aacgtttatg gtccaaatca aaatcctaac tctccatata gcggagttat ttcgattcta
    11881 gtggaccgat acaaaaaaca attggcagga gaaaaaacag aatttacttt atttggtgat
    11941 ggtagccaat ctagagactt cgtttatata gaagatgtta tacaagcact tttacttgtt
    12001 gctaaggaag aaaaagcttt aggacagcaa ttcaatgtcg gtacaggaaa aagcactacg
    12061 ctacttgaat tgattcattc aattgatcaa atcttaggaa cagagctagc gcttaaatat
    12121 gaagcggagc gttcagggga tattcgtgat tctttagcgg atatttcaaa gatccgttct
    12181 ttaggttatc aacccaaatt tgatatattg aacggaatgg aacgttattt aaagacagaa
    12241 atcgattgaa acaaaaaacc agcatcctgc tggttttttt gtctcgactt agctttttaa
    12301 attaacacct tccatcagtt cgtaaatctg atttcttaaa tcagtggccg gcaatttgat
    12361 gtttttatca gattggatat tactttccat gacgattgca tcttttgtgt cttttttcat
    12421 cgccgctact gcatggaatc gattgatatt tccctgagca aggatataat catttccact
    12481 gtaaacaccg ccgctgtaag gatgtttatc accattattt tgccacaatg agaaaagtag
    12541 ttcttttggt ataatatcgc cagacagaac actggtaaag aatttgttca agtcataaac
    12601 agacatactt atatttcctg tgcctaattc atttcggatg tctgtttcag gctcgaccaa
    12661 tgcttttgaa taatcgtcag ttgcagacat ttggtaagat acagcatgct gaggggaatt
    12721 ttttacattt tcataaaaat ctgtatgttc tagatggagt ggccgaatga tttctttttt
    12781 taaaagagtt tcataaggct gattcattag tttgatcaat acccctgtaa gcaatgtata
    12841 atttaatggt tcataatgat acgtatcata accagtaaat ttcaaatgat gaagggcaaa
    12901 ctgaatgatc tcactatctg acataggttt ttttggagaa gcggttcgat gaagaccaga
    12961 gcgcatgtaa agcatatttt tgattgagat ttgctgactt ccaggaatct gaggataaaa
    13021 ttcagatagc tttgtgtcaa aagttatctt gccagattga atttgtttta ggataagtaa
    13081 agcagtcatc gctttttgga tcgaaccgat ttgatagtaa agagaaggag aattcgattc
    13141 ttttatgcgt agatttgcgt acccatatcc tttttctaat atcactcgat tatttttgcg
    13201 tacataaatc gaaccgatat aatctgattt ttgcagtaat tgatcaattt tgctggcaag
    13261 catctgctca ttagatgtta aattggaagc aagttgtgcg ggttccgttt tataaatttg
    13321 tggttgaggt tgttttgtta attgccatcc tgtaatgatg cctgttgaaa agaagctgac
    13381 agccagcaac aaaactgcga gaaaagaaag agagatctgc tgtttttttt gatgttttcc
    13441 ttttgccata ttaaatcctt cctatccatt tttaactatt tttcattgta atcctcaaaa
    13501 agagcatttg ccacttacaa ttttgacata atatttggta aaataattca cggcagtaga
    13561 aatttcatgt ataataaaca gagttacgaa gatgatagta aggaggagtt gttttgtcag
    13621 gaaacaaaca acacccaagc aaagacaaaa aagaagccag taaggcagct acagcagata
    13681 taatcgacaa gcggagaaat ctgcgtaaaa aagaagataa gatcgtgagg aagatcattt
    13741 tagtgatcgc tcttacatta ttgattattg gcggatttct cgggtttacc gtatatcgtt
    13801 atgtagatag cgggttgaag cctttagata aatcggacga ccaattagtc caagtggaaa
    13861 tcccaagcgg ctcttctaat aagcagattg gcgaaatttt ggagaaagac aatattatta
    13921 aaagtgggat tgttttcaat tattatacaa aatttaaaaa cttaactggc tttcaagctg
    13981 gttattatca gttagcgcct aatatgactc ttgatgagat tggcaagcag ttgcaagaag
    14041 gcggcacatc tgagccaaca aaagttgcag atgggaaaat tgccattcca gaaggctacg
    14101 acatcgatca gattgctgag cgggtagcga aggtaactgg taaagacaag aaagaatttt
    14161 tagatttagt aaatgatgag actttcttta acgagttgta tcaaaaattc ccagaattgt
    14221 tggaaagtgc ctctaaagct gagaacacaa aataccgttt ggaaggctac ttattcccag
    14281 ctacttatga ttactacacg agtacttcgc ttaaggatct tgtgattgag atggtgaaca
    14341 aaacaaatac ggtcatgcaa aactattatt cagctatcaa acagaaaaat ttgactgtac
    14401 aagaagtttt aactttggct tctctagttg aaaaagaagg cgtaaaagaa aatgatcgta
    14461 aaaatattgc acaagtcttc tttaaccgaa tcaaggcaaa tatgccactt cagtcagata
    14521 tctctgtttt atatgcatta ggagaacata aagaattggt gacgtatgaa gatacagctg
    14581 tagattcacc atacaatttg tataccaata ccggctatgg gcctggtcct tttgacaatc
    14641 caagcgaaga ggcaatcaag gcagtattag aaccagcaga aaatgactat tattatttcg
    14701 tagcagatac gagcactgga aatgtttatt tcgctaaaac gtatgaagaa catatggaac
    14761 tagtgcaaaa atatgtaaat aattcttaga aaaacagggg ctagtcattg atcattttta
    14821 gaaaaacatt tacattttaa gaaaagcgtg gtaatctttt ttggactata ttcctcaaaa
    14881 gattaccttt tgcggtattt tatcgaaaaa aggagacgtt acacatggta gagaaagttt
    14941 atccaatgac tctagaaggt aaagaaaaat tagaaaaaga attagaagaa ctaaaaacag
    15001 ttaaacgcgg agaaatcatt gaacgaatca aaatcgctcg cggtttcgga gatctttccg
    15061 aaaactccga atacgaatca gctaaagatg aacaagcatt tgttgaagga agaatcacaa
    15121 caattgaaaa tatgatccgt tttgcacaaa tcatcgataa tgacggcgta gatgcagatg
    15181 aggtatcgat cggtaaaaca gttacgttca tcgagttgcc tgatggagat gaagaagagt
    15241 acacgattgt cggaagtgct gaagcagacc cgttttctgg aaagatttcc aatgattctc
    15301 ctatcgcgca agcattgatt ggcaaacgta aaaatgatga agtaaccatt gctacaccag
    15361 gtggcgatat gttagttaaa atcgtcaaag tagaacaagc gtagtctgac tgaaagatct
    15421 agagagttca tcttactgat gacgcactct agatcttttc tctttgagta ttgcaggttt
    15481 cactgaacga tcttacatct aaagtcctat agccaacagt caaaaaaata ggtatgatga
    15541 aatgagaatg tgtcaggagg gagaaaaagc gatgaatagt tcttggcgtt tttttgtggt
    15601 agtcgaagca ctgctgctgt tatttgctgt ttggcaaatc gtaaacaata ctggactttt
    15661 gcttttagta atattcggta ttttcaatat atatctagct atgcgcaaat atcctcgtac
    15721 taaatttcaa aattttcaat tgatcttagg gagcttggtc atttttttta gcttagtcaa
    15781 cagtcctgct ttatggatga tggttgtatt ggccgtttta tttatcgggc tgaaaggtgt
    15841 agagatttca ggaatagatt tgacaaaaaa tgctttttgg cgaaaaaaac agatcatgat
    15901 ggtccaaaca gagcaattaa aaacacataa taatgagcgt aaaagacagc aattatttgg
    15961 taaccaacgt attggaaacg acgtctatga atgggatgat atcaacattg ccatcatttc
    16021 tggtgatact atcatcgatt tgggcaatac acttttgcca aaagatgata atattgtgat
    16081 tgtaagaaaa gggataggca gaacacggat cttagtaccc ctgggagtgg ctattcgttt
    16141 agagcatgcg acattagtag gaaatgtctt gtttgaagaa gagcagtttt ccttaaaaaa
    16201 cgaacagatc aaaatctgca gcagtgatta tgatgaaaat cctcgcagat tgaagatcat
    16261 taccaatact ttggttggag atatcgaggt gatacgaata tgatgggaag aatatccaaa
    16321 gcaatgctag ctgtttattc ggggattgcg gctttcctta ttattctatt ttcacttttc
    16381 acttattttt atgccagtaa ccaaagtcat tggtggggag aattgctgcg tgcgcgttta
    16441 ttatacgttc cgcttatctt ccatttgatg gcaatttcat taggtgtagg attgatcgta
    16501 tttctattgt tatcacttat tcaaaaggca aaatacggga aaatcgaaga aaagcttcgt
    16561 gcactttctt ctggcaatta tgaatccaaa cttttgcatc agccgatccc aagcgcgtcg
    16621 gatgatctgt acatcaaaga tattgatatg gaaattacta aaataaaaga aaaaatgatc
    16681 gaagtatcca gtgaattaca gattatgact agccgtcctc aatatgtaga tggacaaacc
    16741 aaagaagaga ttttaaaatt agaaaggcat cgattagctc gtgagcttca tgattcagtt
    16801 tcacagcaat tatttgcagc gatgatgatg atgtcggcat tgacggagca agcagaaaaa
    16861 agtgagacac cagaaatgtt ccgtaagcag ttgaaaatgg tggcagagat catcaacgct
    16921 tcccagtctg agatgcgtgc gctgcttctt catctgcgtc cagtcaattt agaagaaaaa
    16981 agtttaaaac aaggcattga gcagttattg aaggaattgc agaataaaat ccagatttcg
    17041 ctaaaatggg aggtagaaga tgtaaaatta actagttcca ttgaggatca cttgttccga
    17101 atcgttcaag aattgttatc aaacacatta agacatgcta aggctaatga attagaagtc
    17161 tatttgcata aaatagataa caatctttta ttacgtatca ttgacgacgg gacaggattt
    17221 aatatgaatg aaaccaaaac gggaagttat ggattgaaca atatcaaaga acgagtagct
    17281 ggtattggtg gaacagtaaa aatcattagt tttaaagggc aaggtacgag cgtggagacc
    17341 aaggtccctt tgatgaagga ggcatagata aagatgataa aagttttatt agtagatgac
    17401 catgaaatgg tacgcttagg cgtctcttct tatttatcta tccaagaaga cattgaagtg
    17461 attggagaag ctgaaaatgg acgtcaaggc tatgaaaagg cgatggcact tcgaccggat
    17521 gtcattttaa tggatctagt catggaagaa atggatggca tcgaatcaac aaaagcaatc
    17581 ttaaaagatt ggccaaaagc caaaatcatt atcgtcacaa gttttattga tgatgaaaaa
    17641 gtttatcctg caattgaagc aggagcagca ggatatttgt tgaagacgtc tacagcacat
    17701 gaaatagctg atgctataag ggctacgcaa cgcggcgagc gagtactaga acctgaagtg
    17761 acaacaaaaa tgatggaaaa aatgagtcgg cgaaatgatc cagtattgca tgaagaatta
    17821 acgaatcgag aaaatgaaat tttgatgctg atttctgaag ggaaaagtaa tcaggaaatc
    17881 gcggatgaat tgtttatcac actgaaaaca gtcaaaacgc atgtgtcgaa tatattagct
    17941 aagttagagg tagaagaccg aactcaagca gctatctatg cgtttaaaca tggtttggtg
    18001 aaataaaaac gatgataaag aggataatca aaagctgaga aaataactat tatttttctt
    18061 ggctttttta caataatgat gcttgctttt gtttttggaa aggctttgtt atactgagaa
    18121 aagaagatac taaaggagga aaatggtgaa acaaaatttt gcaattgtag gccttggacg
    18181 atttggcgga agcatctgcc gtacactgat cgaagctgga caagaagtac tggctattga
    18241 cagtaatgaa gatcgagtca atgaatatat gaatatagcc acccatgctg ttgtggcaaa
    18301 tgcacaggat gagatgacac ttcgttcatt aggtataaga aatttcgatc atgtaattgt
    18361 cgctattgga gaagatatcc aagccagcat cttagttact ctgatggtaa aggaaatggg
    18421 cgtaccgaat attttagcaa aagcccaaaa tgagtaccat gcccgtgtac tagagaagat
    18481 cggagcagat cgtgtcgtac accctgagcg agacatgggg atacggattg cacataactt
    18541 agtatcaaag aatattctag actacttaga gctgtcagat aaatattctt tagcagaaat
    18601 ccgagtctcc aatcctaaat ttttcaacaa aacattagct gaattaaatt tcaggcaacg
    18661 ttttgactta acagtagtcg ccatacgaag agtagatcaa acggttatcg cttctcctgc
    18721 agcagatgaa tatgttcgtg aaaatgataa tctactggtt attggagaga caggcgatgt
    18781 ggatattcta gacgataaga tgaatcaata gaaagaaaag agaaagtagg ttgttgggat
    18841 gaaattaacg gtaacgccaa aagcacaaaa gtggtttgac tcagaaattg atctgccaga
    18901 aacttacgga atacgttttt ttggaaaagt ctacggcaaa acagaggttc atgatggttt
    18961 ttcgattgga atgtcagttg aacagccaga acatcctgta aagaaagtgg aaatggatgg
    19021 gacacttttt tttatcgagg aagcagatga atggttcttt aaaggatatg atttacttgt
    19081 tgattatgat cccaacctgg aggagccaac ctatcacttt aaaaaacaat aaaaaaagag
    19141 agggtgacac aatttttgtc accttctctt ttttcttttg aaaatattgt tttcagctat
    19201 taatatcctg cagacaattt tacttgattg gtttgaagct catgggtggc aaagtagctt
    19261 ttcagattgg ttaaaaaaat agccattaat ttcttttgaa aat
//
LOCUS       DACIWG010000048        18896 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.050, whole genome shotgun sequence.
ACCESSION   DACIWG010000048 DACIWG010000000
VERSION     DACIWG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.050"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(22..252)
                     /locus_tag="IYF54_001963"
     CDS             complement(22..252)
                     /locus_tag="IYF54_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832287.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IYF54_001964"
     CDS             complement(688..1254)
                     /locus_tag="IYF54_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ9832288.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IYF54_001965"
     CDS             complement(1254..2210)
                     /locus_tag="IYF54_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ9832289.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IYF54_001966"
     CDS             complement(2210..2866)
                     /locus_tag="IYF54_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ9832290.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IYF54_001967"
     CDS             3236..5254
                     /locus_tag="IYF54_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ9832291.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IYF54_001968"
     CDS             5681..8095
                     /locus_tag="IYF54_001968"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ9832292.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IYF54_001969"
     CDS             8443..9996
                     /locus_tag="IYF54_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ9832293.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IYF54_001970"
     CDS             complement(10377..12161)
                     /locus_tag="IYF54_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832294.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IYF54_001971"
     CDS             complement(12176..13897)
                     /locus_tag="IYF54_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832295.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IYF54_001972"
     CDS             13997..14758
                     /locus_tag="IYF54_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9832296.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15725..15993
                     /locus_tag="IYF54_001973"
                     /pseudo
     CDS             15725..15993
                     /locus_tag="IYF54_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16665..>18896
                     /locus_tag="IYF54_001974"
     CDS             16665..>18896
                     /locus_tag="IYF54_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832297.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5552 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    15361 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    15421 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    15481 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    15541 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    15601 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    15661 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    15721 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    15781 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    15841 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    15901 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    15961 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    16021 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    16081 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    16141 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    16201 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    16261 gatgaaggtt aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc
    16321 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    16381 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    16441 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    16501 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    16561 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    16621 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    16681 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    16741 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    16801 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    16861 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    16921 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    16981 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    17041 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    17101 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    17161 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    17221 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    17281 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    17341 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    17401 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    17461 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    17521 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    17581 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    17641 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    17701 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    17761 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    17821 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    17881 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    17941 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    18001 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    18061 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    18121 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    18181 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    18241 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    18301 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    18361 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    18421 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    18481 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    18541 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    18601 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    18661 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    18721 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    18781 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    18841 ttcaaggagc tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DACIWG010000049        18499 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.051, whole genome shotgun sequence.
ACCESSION   DACIWG010000049 DACIWG010000000
VERSION     DACIWG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.051"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            68..3721
                     /locus_tag="IYF54_001975"
     CDS             68..3721
                     /locus_tag="IYF54_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ9832298.1"
                     /translation="MKKKKSVWFMMVAILMGFIPQILTAYSSIAQAIETQPDKIVDQS
                     GLKVSSSVSSDGNSLNWELQYEKSVASDGNDQALKFKVTADGEAIAFNEDASFAVDDD
                     QWFAEKDFSKQSQGSLSFTTVLNVSKVALEIQADATKTDGTQAVTINKNILTSAVEGP
                     HALKLPAVATQKTTEVSATSQTTAEETTSSQATETTTQTEEASEAKSSEINASPATVG
                     ASNLEYLSGISAFSSINYSNIAPEYTTDATKGTYPTNSWQPAGQTNVINHQGNKERNF
                     ATWDGVTSWNGDPSDTTHSYIQYGEKQTDFAIRKYAKETSKPGLYDVYLNVKGNKQED
                     IKPVDIVLVVDMSGSMESSQSNGWNDRAGAARNGVKNFLQTIKDAGIGDYVNVGLVGF
                     SSPGYVTGPNGYLTVPIGKASDTSHINAINDALKPKFTGGTYTQIGIEQGQQMLAGSS
                     NENKMMIVLTDGVPTFSKKVTAAQTIDGTTYATEFGNRLDEPSTTSGLPWWDRYTVDR
                     TLINSTWPATLGAAKIAKDAGLTIHLLGIQLSKDRNFLTEQQVRDRASLIATPGKYKD
                     AETTDDVSDYLNEQAKNVVKSFNTIVNGSINDSLGDQFSYEGTPTVKSISTGDSAVTN
                     LPTVNQSNGKVTTTELNLGKDQEIQIHYQVRINTEDKDFTPEKWYPMNGQTTLTPNGD
                     NPDNKVDFGVPSAKAPGVKLDLKKIWEEYDKDTSKRPDSLNFTLNRKDTTESNSWKQG
                     FIRLSKSDVATDTNVWEKTNIEKVAETQGATESLWLPQFNNKGKDFAYSFEEATVNGY
                     ESSSNAATTIWTNKKIYTPLALEITKISDQGKTPLKGAVFKLSGGSLPNAGVELKDNG
                     DGTYTLSDDKYKLQLNTEYTLTEVTPPAGHTATTTSWKVDVSAEGKVTINDAVPGIEI
                     KDNTIKYTIENEFTKRPLLVEKYNKDSGKTLDGAKFTLKQYDDKWTNDGKVVGDDLIG
                     NDAKSFASLAPGYYSLEETKVPTGYKKDDTVFKFQIDSAGKLLDANGKEITANSKPTT
                     DGFYLTSDNKIVLIKYNELRDFDFSILKKNSQSNKPLADTEFSLATKEDSNTILATLK
                     TNDEGTGQFLDASGNHYGVRPGTYVIKETKAPEGFVLLKGIFEVTINADGTVGDVTYD
                     GKDLDKDAIKVNLKDEDNNVIELTVANTPKGQLPATGGSGIQTFIIVALALVTAAGAL
                     TICYFYRNRKELN"
     gene            3723..4454
                     /locus_tag="IYF54_001976"
     CDS             3723..4454
                     /locus_tag="IYF54_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ9832299.1"
                     /translation="MKNKSLFIKITILLSTLLVGIFTGQQAFADDGTIDIVIHKQLWD
                     QAPDAAIQNTGAADQDIKDPIEGVGFTIYDITEDYYKLVKGSDETTTIQMIQENVADY
                     QKIIREQGLTDEKSTIKFTQLPKSSGGKNAVYLIVETALPDLEKVKVEKAAEPLVVAL
                     PVYEMSTDGKTYTDKELATVHLYPKNVGTIVEKPVTPTKPKKPSKPKKHLPQTGEAKS
                     FLGILGILLIGTAIILWRKHSVRNN"
     gene            4519..6006
                     /locus_tag="IYF54_001977"
     CDS             4519..6006
                     /locus_tag="IYF54_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ9832300.1"
                     /translation="MKKLWKTLFAALLVVPLFTGVFGEKTVSATEATTTNVTINKRIW
                     KDGEAPAQGSMQNTGEEMDFGGDPLEGAGFTAYDVTDAYLALIADGKSQAEATKEIAD
                     NASDYTTVAKAEQKTDANGQTTFEGLALKDGDKDKVYMFVETSTPGNVSVTTKAAPIV
                     LAMPIYTFKDGKYSDTINTNVQVYPKNETKTDKKEVANLDKFTEVKDADGNVLYHNLT
                     TGDEIEYKLTLNIPADILEDGVTYSVTDTPTAGLAYVKDSFAATGLVAGTDYELAEDS
                     ATGGFTVTLKQSENVGKLAGSQLIATYKMKLTAEVNPDDLVNNSAQVTIGNNPQDKIT
                     PPTQFGTGGYKFVKKDSQSGATLSGAEFVVKQGSNFAIFEDAKNTKGEYIFKEWTTDE
                     AKATKIISDDKGELKVIGLKNGDYQLEEKATSSDKYVLLDEDVDFTVEHGQYGSQELK
                     SVLNTPKGLLPSTGGNGIYAFLLIGAALMIGAYAWFKKSKTQAEV"
     gene            6092..7288
                     /locus_tag="IYF54_001978"
     CDS             6092..7288
                     /locus_tag="IYF54_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9832301.1"
                     /translation="MDQRQKNERINLILKLLMAILFTTGAIIFSYPFLSNAVNSYYDQ
                     KMMEKNLAEMAATNTKEQAKRHQEMAEKNKELAESKNMTNIPGMGLVEDPFENAVDDA
                     KDPGKAYYEEHTVGAIYIPKISVSLPLFDETNAALLEKGATILQGTSYPIGGDSTHSV
                     ISGHSGLTERKLFTDLEKLVIGDDFYLTIAGEKLAYAVDDIQIVLPSDIDKVVIQPGR
                     DLVTLLTCTPYGINTHRLLVTGHRVPYVEEMAEEVTSTKKAVERRFRLYLLLIPLFFA
                     MVFYWMYRKFVYYQSGKHSYDFCFYLLENGQPKAGVTFTLVRKKRWATDVTSQPVAVS
                     QVDGWVSFPEIRGGRYYAKAIDGSTKPVKGKVRRLKDRQFVLSRVTKKKHGKKVTYYL
                     ENGAKK"
     gene            7285..8406
                     /locus_tag="IYF54_001979"
     CDS             7285..8406
                     /locus_tag="IYF54_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ9832302.1"
                     /translation="MKKNRQVISQIAIILIFMVGILTMLYPFYVDALNNVIDQVRVDR
                     YLKESQKEFEAERKRLAEENSKLSESGLAPGADPFANPNGGTVSAAYYKKHLIGTINL
                     PDLAIELPLFDTTNDDLLEQGATVLDGTSFPVGGASTHAVISAHRGLPERELFTNLPE
                     LKNGDIFLLNVLGETLAYEVFDSQVVTPDQTSVLKIEPGQDLVTLMTCTPYMINSHRL
                     LVTGKRVPYTPAAEKKQVKGDRFRKLKQIAILAGTALLILAAIYQLYHVIARYRLRKV
                     RFDFTVCLEGVAEHTPIALYDKKGKKALRRNGKAYQELTDQTGQVTFTDLPGDCYRLK
                     LGKSWLVQFGLKKKKRPSKIWKINKKKVMLKEERMLEVK"
     gene            9127..10119
                     /locus_tag="IYF54_001980"
     CDS             9127..10119
                     /locus_tag="IYF54_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832303.1"
                     /translation="MSKDNKDDDLVKNIRKYATTKRLNFLLGSGVSYPAIPLMGSIEG
                     KSEEEKNGKLLEKVKLVSDKLVTNEFCNDTDIYYTLRMYREFILNIIGILHLSNSRQI
                     PRTANIFTTNYDLFIEKATDKVLKNFKFVFNDGASGYFDRKLESTNYNRTVSYKGLND
                     NYTNELPSLTLIKPHGSVNWEKNEEFLYIRNHVIANPCVVKPTGREEEETFLSNHFHE
                     MLRIFQLELDKPQTILFVIGFSFQDKHIAKMLKRALQNPELMTYIFAFDEVDKDKITS
                     NLSHEVGQYNLNILIPKDFSENHKQKNDKGAEFFTLKNLNSILNNVTIGDSKYD"
     gene            10112..11992
                     /locus_tag="IYF54_001981"
     CDS             10112..11992
                     /locus_tag="IYF54_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9832304.1"
                     /translation="MTNPSKKLGVITAVDGDIAQVGMYNMSNDSEFFWYGETLIGPKI
                     GAYLTINQNDNKIIATVINEKIIDQQNSIKSEEFDNRYSKQSINRIISLKTKGVIEND
                     KFQVTSQYVPMIGNEVSLTNMEDLKIIYGINNAKDSIKIGYSILEKQPISLSINKFFA
                     SHIGIFGNTGSGKSNTLHKLYLELFKSEYYEKIVEKSQFFVIDFNGEYISSKTNEKTQ
                     KGIFGVHKDNRTLFKINTRNEDKSSKIPVKKEYLFDADILSILFDARSATQVPFLRSA
                     VREFMKKQNEPFFDFGSYVTGTLKKILTSGLSVNQDSLQDWLDIAKKYLKRDLEFKYL
                     DQLVQFNNGDFKINSLDIWFSKGVETNISDDILTRSGINNISVMLKEYFDNNKDTPLQ
                     QLRIFLDFQKVHRTAWGKINREHLNPLFNRINSSLESLEKVIEIKEDAIGCYKTLNII
                     SLVHANQEITRLVPMLLSKMIYDEQKSKTAGKKVSGTKHLIIDEAHNILNSEYKNNGD
                     SWQDYRLSVFEEIIKEGRKFGFYLTLSSQRPADISPTILSQTHNYLIHRLVNENDLRM
                     LEKTMPTLDKTSYNFIPSLGKGEAIITGSALSIPVLVKIDKETDLRPSSDDINLTSLW
                     ND"
     gene            complement(12021..12626)
                     /locus_tag="IYF54_001982"
     CDS             complement(12021..12626)
                     /locus_tag="IYF54_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832305.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLENGVAISKISKEYNVTRQTVYRIKNELNT"
     gene            12821..13873
                     /locus_tag="IYF54_001983"
     CDS             12821..13873
                     /locus_tag="IYF54_001983"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HAQ9832306.1"
                     /translation="MSENVYYSAKEFYAKINQDLTIRREDMVILVCSIVIACIGLNMD
                     TTPVVIGAMLVSPLMTPIQGIGMGLSLFDQQMLKKALKILLVQIMVSIVTASIYFFIS
                     PISYASNEILARTQPTIWDVIIAFFGGIAGVIGSRKKAMNNIVAGVAIAAALMPPLCT
                     VGYAIALKKMPYFLGAGYLFLINCFFIIIATFIGVQVMRIPKSVKVDSEEKSKMNVVI
                     VILSIVILIPSIISASQLVGKSIIENGLRRMLSEELGNNVIVINKSIDQENKIIYLTT
                     SGEKISDTRIKKIEESLPMYNLKGMSLEISQITNTQQLSGEDLVRLLKEYEESKKSNS
                     IEKTKRVNSTEDSNNE"
     gene            14444..15028
                     /locus_tag="IYF54_001984"
     CDS             14444..15028
                     /locus_tag="IYF54_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832307.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSLQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLKAQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLDKMEGKDGKTNTH"
     gene            15006..15221
                     /locus_tag="IYF54_001985"
     CDS             15006..15221
                     /locus_tag="IYF54_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9832308.1"
                     /translation="MEKQTLTSFSEQQRIAAMKKYKIIEPYLNKQKTIKEIAIKNKAP
                     TRTLYRWVQKYEHDGLVGLIRKTRTDF"
     gene            15542..17686
                     /gene="topB"
                     /locus_tag="IYF54_001986"
     CDS             15542..17686
                     /gene="topB"
                     /locus_tag="IYF54_001986"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ9832309.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGQYNEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLSFVSSNHAKIGNQEGRIADV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITE
                     NEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGEHAEVDVKSAEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFITNIKKFIVHLLEAVPNDIEKLNFSDYQEQKEKE
                     AEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            17814..18053
                     /locus_tag="IYF54_001987"
     CDS             17814..18053
                     /locus_tag="IYF54_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ9832310.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            18102..18185
                     /locus_tag="IYF54_001988"
     CDS             18102..18185
                     /locus_tag="IYF54_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ9832311.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            18310..>18499
                     /locus_tag="IYF54_001989"
     CDS             18310..>18499
                     /locus_tag="IYF54_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9832312.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHL"
BASE COUNT         6568 a         2957 c         3704 g         5270 t
ORIGIN      
        1 aatcttaggt agtgctattt aaattttgtt taagaataga tatcatgttt tttagaggag
       61 ggaagaaatg aagaagaaaa aatcggtctg gtttatgatg gttgctattt tgatgggctt
      121 tatcccgcaa atcttgactg cttattcgag tatcgcacag gctatagaaa ctcagccgga
      181 taaaatcgta gatcaatcag gtttaaaagt cagttcctct gtttcatcag atggaaattc
      241 tttaaactgg gaacttcaat atgaaaaaag tgtagcaagt gatggcaatg atcaagcact
      301 gaaatttaaa gtgacggctg atggagaagc aatagctttt aatgaagatg ccagttttgc
      361 tgttgatgat gaccaatggt ttgcggaaaa agatttttca aaacaatcgc agggttctct
      421 ttcattcaca acggtgctaa atgtctccaa ggtagctctt gaaattcaag ccgatgcaac
      481 gaaaacggat ggaacacaag ctgtcactat taacaagaat attttgacta gtgcggtaga
      541 aggtccgcat gcgttgaaat taccagcagt ggctacacaa aaaacgacag aagtaagtgc
      601 tacttctcag acaacagctg aagaaaccac tagtagccaa gcgaccgaaa cgacgacaca
      661 aacggaagaa gccagtgaag caaagtcgtc tgagataaat gcctcgccgg caacagtggg
      721 cgctagcaat cttgaatatc ttagtggaat aagtgcattt tcttccatta attacagcaa
      781 tattgcgcca gaatatacga cagatgccac aaaaggaact tatccgacta atagctggca
      841 accagctggg caaacaaatg tgatcaacca ccaagggaac aaggaacgca actttgccac
      901 ttgggatggt gtaactagct ggaatggcga tccatcagac acaacacatt cttatatcca
      961 gtatggtgaa aaacaaacag attttgccat tcggaagtat gccaaagaaa cgagtaaacc
     1021 agggctttac gatgtatatt tgaatgtaaa aggtaataag caagaagata tcaaaccagt
     1081 tgatattgtt ttggtagtgg atatgtcagg aagtatggaa tcgagtcaaa gtaacggctg
     1141 gaatgatcgt gctggtgcag ccagaaatgg tgtgaaaaat ttcttgcaaa ccattaaaga
     1201 cgcaggcatt ggggattacg tcaatgttgg tttagtcgga ttttctagtc cgggatatgt
     1261 aacagggcct aacggttatc ttactgtacc gataggtaaa gcttcggata ctagccacat
     1321 aaatgcaatc aatgatgctt taaaaccaaa atttacagga ggtacttaca ctcaaatcgg
     1381 gatcgagcaa ggtcagcaga tgcttgcggg aagctccaat gaaaacaaaa tgatgatcgt
     1441 attgaccgat ggggttccta ctttttctaa gaaggtaacc gctgcgcaaa cgattgatgg
     1501 gactacctat gctactgaat ttggtaatag attggatgaa ccaagcacta catctggact
     1561 cccatggtgg gataggtata cggttgatag aacactgatt aatagtactt ggccggccac
     1621 attaggagca gctaaaatcg ccaaagatgc gggcttaact atccacttgc tgggaattca
     1681 gctgagtaag gatagaaatt ttttaactga acaacaagtt cgcgatagag caagtcttat
     1741 cgctacaccg ggtaaataca aagatgctga aacaacagac gatgtttctg attatttgaa
     1801 tgaacaagct aaaaatgtag tgaaatcttt caacaccata gtcaatggtt cgatcaatga
     1861 ttctttgggt gatcagttct cttatgaagg cactccaact gtgaagagca tcagtacagg
     1921 ggatagtgct gtaacgaatt taccgaccgt aaaccagtcg aatggtaaag tgaccactac
     1981 tgagttgaac ttaggcaaag atcaagaaat tcagatccat tatcaagtgc gtatcaatac
     2041 agaagataaa gatttcacgc cggaaaaatg gtatccgatg aatggtcaaa caactttgac
     2101 accaaacgga gacaatccgg ataataaagt agactttggc gttccttctg caaaggcacc
     2161 gggggttaaa ctcgatctga aaaagatttg ggaagaatac gataaggata cgagtaaacg
     2221 tcccgattct cttaatttca ccttgaaccg aaaagatacc acagagagca attcttggaa
     2281 gcaaggtttt attcgtcttt ctaaaagcga tgttgctaca gatactaatg tctgggaaaa
     2341 gaccaatatt gaaaaagtag ccgaaacaca aggagctact gaatcgcttt ggttgccaca
     2401 attcaacaac aaagggaaag attttgctta tagctttgaa gaagcaacgg tcaatggcta
     2461 tgaatcttca agtaatgctg ctacaaccat ttggaccaat aaaaaaatct acacaccact
     2521 tgctttagag atcactaaaa tttctgatca aggaaagaca cctcttaaag gagcagtctt
     2581 taaactaagt ggaggtagtt tgccaaatgc aggcgttgaa cttaaggaca acggggatgg
     2641 aacctatacg ttgtctgatg ataagtacaa gctgcaatta aatactgagt atacattgac
     2701 agaagttaca ccaccagctg gacatacggc tactacaact agttggaaag tcgacgttag
     2761 tgcagaaggt aaagtaacca tcaacgatgc tgtaccgggt attgaaatca aagacaacac
     2821 tatcaagtac accattgaaa atgagtttac caaacgtcct ttattggttg aaaaatataa
     2881 taaagatagt ggtaaaacat tagacggtgc gaagttcacg ttgaagcaat atgacgataa
     2941 gtggacgaat gatggtaaag ttgtcggtga tgatttgatc ggaaatgatg ccaaatcttt
     3001 tgctagcctg gcaccaggct attacagctt ggaagaaacg aaggtaccga caggctataa
     3061 gaaagacgat acagtcttta agttccaaat cgatagcgct gggaaactac ttgatgcaaa
     3121 tggcaaagaa atcaccgcta actcaaaacc aacgacagat ggtttctatt taacgagtga
     3181 taataagatt gttttaatca aatacaatga actgcgtgat tttgactttt caattcttaa
     3241 aaagaactcg caaagcaaca aaccattagc agatactgaa ttcagcttag ccacaaaaga
     3301 ggattccaat acgattttgg ctactctgaa gaccaatgat gaaggaacgg gtcagttttt
     3361 agatgcttcg ggtaatcatt acggtgttcg tccaggaact tacgttatca aagaaacgaa
     3421 agcaccagaa gggtttgtcc ttttaaaagg gattttcgag gtcactataa atgcggacgg
     3481 aactgtcggc gacgtgactt atgatggtaa ggatcttgat aaagatgcta tcaaagtaaa
     3541 tctaaaagat gaggacaata acgtcatcga attgacggtt gctaatacac ccaaaggaca
     3601 actgccagca accggcggat caggtataca gactttcatc atagtagcct tagctcttgt
     3661 cacagcagca ggtgctctta cgatctgcta cttctaccgt aatcgaaagg agttgaacta
     3721 agatgaagaa taaatcattg ttcatcaaaa tcaccatact tctttcaact ttgctggtag
     3781 ggatatttac ggggcaacaa gcctttgcgg atgacgggac gatcgatatt gtgatccaca
     3841 aacaattgtg ggaccaagca cctgatgcag ctattcaaaa tacgggagcg gccgatcaag
     3901 atatcaaaga tccaatcgaa ggggtcggct ttacgattta tgacattaca gaggattatt
     3961 ataaattggt caaaggctct gacgaaacaa caactattca aatgattcaa gaaaatgttg
     4021 ctgattatca aaaaattatc agagaacaag gtctgactga cgaaaaaagc acaatcaaat
     4081 ttacccaact acctaaaagc agtggcggta aaaatgcggt ttacctgatt gttgaaacgg
     4141 cattgcctga tcttgaaaag gtaaaagtcg aaaaagcggc cgaaccctta gtagtggctt
     4201 taccagtata tgaaatgagc acagacggta aaacctatac ggacaaagaa ttagctaccg
     4261 ttcaccttta tcctaaaaat gtgggaacaa tcgttgaaaa gccagtgaca ccaactaaac
     4321 ctaaaaaacc aagtaaaccg aagaaacact tgccacaaac aggtgaagca aaatccttcc
     4381 taggtatatt ggggattctt ttgatcggta cggcgatcat tttatggcgg aaacactctg
     4441 tacgaaacaa ttaatcaaag gtacaaaaga ctaaggtcta ttgaaatatt gtacaaaaaa
     4501 acaaggagga attagaaaat gaagaaatta tggaaaacac tttttgcagc actacttgta
     4561 gtacctttat tcaccggcgt gtttggtgag aaaacagtaa gtgctactga agcaacaacg
     4621 acaaatgtaa cgatcaacaa aagaatctgg aaagatggcg aagctccagc acaaggttcc
     4681 atgcaaaata ctggggaaga aatggacttt ggtggagatc cacttgaagg agccggcttt
     4741 acagcctatg atgtaacaga tgcttatctt gctctgattg cagatggtaa gtcacaagca
     4801 gaagcaacaa aagaaattgc tgataatgcg tcagattaca caactgtagc aaaagcagaa
     4861 caaaaaacgg atgccaatgg tcaaacaact tttgaaggac ttgctttaaa agacggcgac
     4921 aaagataaag tctatatgtt tgttgaaaca agcacgccag gcaatgtttc cgtaacgaca
     4981 aaggccgctc caatcgtttt agcaatgcca atctatacgt ttaaagatgg caaatattca
     5041 gatacaatca acaccaacgt tcaagtctat ccaaagaacg aaaccaagac tgataaaaaa
     5101 gaagttgcga atttagataa gttcactgaa gttaaggatg ctgatggaaa tgttttatat
     5161 cataacctta caactggcga tgaaatcgaa tataaattaa ctttaaatat tcctgcggac
     5221 attctggaag atggcgtcac ttactctgta actgatacgc caactgctgg acttgcttat
     5281 gtaaaagatt cttttgcagc aactggttta gtagctggaa ctgactatga attagccgaa
     5341 gattctgcaa ctggcggatt tactgttact ttgaaacaaa gtgaaaatgt aggaaaatta
     5401 gctggtagtc aattgattgc tacttacaaa atgaaattaa ctgctgaagt aaatcctgac
     5461 gatttagtaa acaatagtgc ccaagtaaca attggtaaca accctcaaga taaaattaca
     5521 cctccaacac aatttggtac aggtggttat aaattcgtca aaaaagattc gcaatctggt
     5581 gcaaccttat ctggtgcgga atttgtcgta aaacaaggct ctaactttgc aatatttgaa
     5641 gatgctaaaa acactaaagg tgaatatatc tttaaagaat ggacgactga tgaagcaaaa
     5701 gcaacgaaaa tcatttctga cgacaaaggt gagttgaaag taattggttt gaaaaatggt
     5761 gactatcaat tagaagaaaa agcgacttca agtgataaat atgtgttact tgatgaagat
     5821 gtcgatttca ctgttgaaca cggtcaatat ggtagtcaag aactaaagtc tgttcttaac
     5881 acgcctaaag gtctgcttcc atcaacaggt ggtaacggga tctatgcctt cttacttatc
     5941 ggtgctgctc ttatgatcgg tgcttacgct tggttcaaga aatctaaaac acaagctgaa
     6001 gtctaagata tagttttaaa atagcaaaag gcagcttcat ttcttgaagt tgccttttct
     6061 ttcttttaaa tagggagaag gaggtgcaag catggatcag cgccaaaaaa atgaacggat
     6121 caatttgatt ttaaaactgc taatggcgat tttatttacg accggtgcca ttattttcag
     6181 ctatcccttt ttgtccaatg cggtgaattc ctattatgac caaaagatga tggaaaaaaa
     6241 cttggctgag atggccgcaa caaataccaa agaacaagca aaaaggcacc aagaaatggc
     6301 cgaaaaaaat aaagagctag ctgaaagtaa gaacatgacc aatattcctg gcatgggact
     6361 ggtagaagac ccgtttgaaa atgcggtcga tgatgccaaa gatccgggta aagcctatta
     6421 tgaagagcat accgtgggtg ctatctatat tccaaaaatt tcggtcagct tacctttatt
     6481 tgatgaaacc aatgctgctc ttttggaaaa aggcgcgacg attttacaag gaacgtctta
     6541 tccgatcggt ggcgatagta cccattcggt gatctcaggc cacagtggct tgacggaacg
     6601 taaacttttt acagatttag aaaagttggt gattggcgat gatttttatt tgaccattgc
     6661 tggggaaaaa ttggcttatg cggtcgatga catccagatc gttttaccgt cggatatcga
     6721 caaagtggtc atccaaccgg ggcgggactt ggtgacctta ttgacttgta caccttatgg
     6781 catcaatacc catcgtttat tagtcactgg acaccgtgtg ccttatgtgg aggaaatggc
     6841 ggaggaagtg accagtacca aaaaagcggt cgaacggcgt tttcgcttgt atttactact
     6901 gatcccgctt ttctttgcca tggttttcta ttggatgtat cgcaagttcg tctattatca
     6961 aagtggcaaa cacagttatg acttttgttt ttacttattg gaaaatggtc aacccaaagc
     7021 aggcgtgact tttaccttag tccgtaaaaa acgctgggca acagatgtca ctagccaacc
     7081 agttgctgta agccaggtgg atggatgggt gtctttccct gagatcagag ggggtcgtta
     7141 ttatgccaag gcgatcgatg ggtcgacaaa accagttaag ggcaaggtcc gccgtttaaa
     7201 agatcggcag tttgttttaa gccgcgtcac gaagaaaaag cacggtaaga aagttactta
     7261 ttatctggag aatggagcga aaaaatgaag aaaaataggc aagtgatctc gcaaattgcg
     7321 atcatcctca tttttatggt cggcatcttg accatgctct atccctttta tgtcgatgcg
     7381 ttgaataatg tgatcgatca agtgcgcgtc gatcgctatt tgaaggaaag ccagaaagaa
     7441 tttgaagccg aacgcaagcg actggcggaa gaaaatagta aattgagcga aagtgggtta
     7501 gctccgggtg ctgatccgtt tgcgaatccg aatggcggta cggtttcagc tgcttattac
     7561 aaaaaacatt tgattggaac catcaatctt cctgatttgg ccattgagtt accgctgttt
     7621 gatacgacca atgatgattt attggaacaa ggtgcgacgg tcctagatgg cacgtccttt
     7681 cctgttggtg gcgcaagtac ccatgcggtg atctcggctc atcgtggctt gccagaacgg
     7741 gaactcttca ccaatctgcc ggagcttaaa aacggtgata tttttctgct aaatgtctta
     7801 ggggaaacct tggcttatga agtgtttgac agccaagtcg tgacgccaga tcagacttcg
     7861 gttttaaaga tcgaaccagg ccaagacttg gtgacgctca tgacctgtac cccttatatg
     7921 atcaatagtc accgtttgtt agtcacagga aaacgcgtgc catatacacc ggctgccgag
     7981 aaaaaacaag tcaaaggcga tcgttttcgt aagttgaaac aaatcgctat cttggcgggg
     8041 acggccctgt tgatcttagc cgctatttat caattgtatc atgtgatcgc ccgttaccga
     8101 ctgcggaaag tgcgttttga tttcacagtt tgcttagaag gcgtggcaga acatacgcca
     8161 atcgcactct atgataaaaa agggaaaaaa gccctgcggc gtaatggaaa agcctatcaa
     8221 gaactaacag atcaaaccgg acaagtcact tttaccgatc taccagggga ttgctatcgt
     8281 ttgaaattgg gcaagagttg gttggtgcag tttggtttga agaaaaaaaa gaggccatcc
     8341 aagatttgga agataaataa gaaaaaagtt atgttaaaag aagaaagaat gctagaagtc
     8401 aaataaaaaa tgctcatgtt atcaaatgat ttaaatccat aagatacacg tttcattgtt
     8461 taatatgggc attcttagct tctattttgt cattggtatg gggataaatc aatgcacttc
     8521 gtataccttc gttatatttg agtaaatttt gtagtttttt ctaaattctt cgtctaaact
     8581 ttctgtaaga agtgggaatt taggtgcacg gaaactacac caagtttttt aatattccgt
     8641 ctgtttttag ttaagaaacg ccaatttact ttcagtttct gtgcttcatt tttgagccct
     8701 tttaattgca gctttttcat cattcttaca cgaaaggctt gaaacggatg ttccagtgac
     8761 ctcgtttaga gcgtacattt attcaagaaa gtttaaataa gatatcaaaa tggccgaaaa
     8821 aagacacaca actcgattta ttttttaggg tgaaggagag agtagcacga aaacttttag
     8881 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
     8941 gaagcgaaca cttgatcttt taagttgcta tcatttatag gtcaatagag tatactctgt
     9001 tgggtgttga actataatta gaatatgtgt attactttga ggtcatcaat attaggcata
     9061 aggggttatt ttataactgt gaacttgtag tataatataa gtaaaatata tatttgggag
     9121 aatacaatgt caaaggataa caaagatgat gatttagtaa aaaatattag aaaatatgcg
     9181 actacgaaaa gattgaattt tttgttagga tcaggggttt catatccagc tattccgtta
     9241 atgggaagta tagagggtaa aagtgaagaa gaaaaaaatg gaaaattatt agaaaaagta
     9301 aaattagtaa gcgataaatt agttacaaat gaattctgta atgatactga tatatattat
     9361 acactaagaa tgtatagaga atttatttta aatattattg gtattttaca tctatcaaat
     9421 tctagacaaa ttcctcgaac agccaatatt tttacaacaa attatgattt gtttattgaa
     9481 aaagcaacag ataaagttct aaaaaacttt aaatttgtgt ttaatgatgg ggcaagtgga
     9541 tatttcgata gaaaattgga aagtactaat tataatagga cagtatctta taaggggtta
     9601 aatgacaact atacaaatga gttaccttct ctaactttaa taaaaccgca tggttcagta
     9661 aattgggaaa aaaatgaaga gtttctgtat attagaaatc atgtcatagc aaatccctgt
     9721 gttgtaaaac caactggaag agaagaagaa gaaacatttt taagtaatca ttttcatgaa
     9781 atgctgcgta tatttcaact tgaattagat aaaccacaaa ctatattatt tgtaataggt
     9841 ttttcttttc aagataaaca cattgcaaaa atgcttaaac gtgcgttgca gaatccagag
     9901 ttaatgacgt atatttttgc atttgatgag gttgataaag ataaaataac aagcaactta
     9961 tcgcatgaag ttggtcaata taatttgaat atattgatcc ctaaagattt ttcggaaaat
    10021 cataagcaaa aaaatgataa aggagcggaa tttttcacat taaaaaatct aaattctatc
    10081 ctaaataatg taacgatagg agatagtaag tatgactaac cccagtaaaa agttaggagt
    10141 aattactgca gtggatggag atatcgctca agtagggatg tataatatgt ctaatgattc
    10201 tgaatttttc tggtatggag agacattaat aggtccaaaa attggtgctt atcttacgat
    10261 aaaccaaaat gataataaaa ttattgctac tgtaatcaat gaaaaaataa ttgatcaaca
    10321 aaactcaata aaaagtgaag agtttgataa cagatattca aagcaatcaa taaacagaat
    10381 tatttctttg aaaacaaagg gcgtaatcga aaacgataaa tttcaagtaa ccagtcaata
    10441 tgttcccatg attggaaatg aagtctcact aacaaatatg gaagatttaa aaattattta
    10501 tggtattaac aatgcaaaag attctattaa aattggatat tctattttag aaaaacaacc
    10561 tatcagttta tcaataaata aattttttgc ttctcatatc ggtatatttg gtaatacagg
    10621 aagtggtaaa tctaatacac tacacaaatt gtacttagaa ttgtttaaat cagagtacta
    10681 tgaaaaaatt gtagaaaaat ctcaattttt tgtgatagat tttaatggtg agtatatttc
    10741 ttctaaaaca aatgaaaaaa ctcaaaaagg tatatttgga gttcacaaag ataatagaac
    10801 actttttaaa ataaatacta gaaacgaaga caaaagttca aagattccag taaaaaaaga
    10861 gtatctgttt gatgcagata ttttatcaat attatttgat gctcgttctg caacgcaagt
    10921 acctttttta agaagtgcag ttagagaatt tatgaaaaaa caaaacgaac cctttttcga
    10981 ttttggatca tatgttacag gaacgctaaa aaaaatatta acatcaggac tttcggtgaa
    11041 ccaagatagt ttacaagatt ggttagatat agcaaaaaag tatttgaaga gagatttaga
    11101 gtttaaatat ttagatcagt tagtccaatt taataatgga gactttaaga ttaattcatt
    11161 agatatctgg ttcagtaaag gtgttgaaac caatatttca gatgatatat tgactcggtc
    11221 tggaataaat aatatttctg ttatgttaaa ggagtacttt gataataata aagatactcc
    11281 tttacaacaa ttgaggatat ttctagattt tcaaaaagta catcggacag catggggaaa
    11341 aattaataga gagcatttaa atcctctatt taataggatt aattcttctt tagaaagttt
    11401 agaaaaagta atagagataa aagaagatgc tatagggtgc tataaaacat taaatattat
    11461 ttctcttgtt catgctaatc aagaaattac tcgtttagta ccaatgttat tgtctaaaat
    11521 gatttatgat gaacaaaaat caaaaactgc aggaaagaaa gttagtggaa caaagcattt
    11581 aataattgat gaagctcaca atatattaaa ttcggagtat aaaaataatg gagatagctg
    11641 gcaagactat agattatcag tatttgagga aattataaaa gaaggaagaa aatttggctt
    11701 ttatttaaca ttatcaagtc aaagaccagc tgatatttcg cctacgattc tttcgcaaac
    11761 acataattat ttaattcatc gtttagtaaa tgaaaatgat ctacgcatgc tagaaaagac
    11821 aatgccaacc cttgataaaa catcttacaa ttttattcct agtttaggaa agggagaagc
    11881 aattataaca ggaagtgctt taagcattcc tgtattagtg aaaatagata aagaaacaga
    11941 ccttagacct agtagcgatg atataaatct tacgagctta tggaatgatt aaatatcatt
    12001 agtattcaat ttatactgta ttaagtattt agttcatttt ttattctata cacagtttgt
    12061 ctcgtaacat tatattcctt agagattttt gaaatagcaa cgccattctc aagatcctca
    12121 acgatgtttt tataaacaag gcgacgttgg ggatcttttg cgttcgcact ataaagctta
    12181 ggtcgacctt tatacacacc attagctttg gcaattttaa ttccttgtgc ttgtcgacgt
    12241 ttggattcag tacgttcttg ctcggcaatc atagctaaaa tttgaatgat caaatcttta
    12301 atgaatttat ccaggagagg atttccaatt gcttcagcca tgatcggtag actggtgatg
    12361 atcagctgca cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt
    12421 cttccaagtc ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa
    12481 gcttcctgga aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt
    12541 ttttctgcgc cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac
    12601 gaaactcgaa tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata
    12661 cgccctaatc tgtcactttt ctatttaaat ggcataaatt tttctgatta tttggcatac
    12721 ctatttctac ttaacgagag attaattatg atataattag ggacgaataa ctatgaattt
    12781 aatatgaaaa taaagaaact tacagttaag gaagaaaatg atgagtgaga atgtatatta
    12841 tagtgccaaa gaattttatg ctaaaatcaa ccaagatttg actatccgca gagaagatat
    12901 ggttatttta gtttgctcaa tcgttatagc ttgtattggc ttgaatatgg atactactcc
    12961 agttgttatt ggagcaatgc ttgtatcccc cctaatgaca cctatccaag gtatcgggat
    13021 ggggttgtct ctttttgacc aacaaatgct aaaaaaagcg ttaaaaatcc ttttagtaca
    13081 aataatggtt tctatagtga cagcaagtat ttattttttt atttctccta tttcttatgc
    13141 aagtaatgaa attcttgcac gaacacaacc aacaatttgg gacgtgatta ttgctttctt
    13201 tggaggaatt gctggggtca taggttctcg aaaaaaagca atgaataata ttgttgccgg
    13261 tgtcgcaatt gctgcagctt taatgccacc tttgtgtacg gtaggttatg ctattgcttt
    13321 aaaaaaaatg ccctattttt taggagcagg atatctattt ttaatcaatt gttttttcat
    13381 tattatcgct acgttcattg gtgttcaagt catgcgtatt cctaaaagtg taaaggtgga
    13441 tagtgaagag aagagtaaga tgaatgtggt tattgttata ctgagtattg ttattctgat
    13501 cccaagtata atttctgcct cacaacttgt tggaaaaagt attattgaga atggattaag
    13561 acgaatgctc tcagaagaat tgggtaacaa cgtcattgtc attaacaaat cgattgatca
    13621 agaaaataaa attatttatt taacaacttc aggtgagaaa atatctgata caagaataaa
    13681 aaaaattgaa gaaagtttac caatgtataa cttaaaagga atgtccttgg aaataagtca
    13741 gattacaaac actcaacaac tttctggaga agacttagtc agacttttaa aggagtatga
    13801 ggagtctaaa aagagtaact ctatagagaa aacaaaaaga gtgaattcta cagaagattc
    13861 aaataatgag taaatggaaa gtagttagaa aatgaactct gtatcggagt tgagcgcggt
    13921 tgatatgaat ttttatatct gtgaagattg cgtattgcct gatagaatga accaaagtat
    13981 tgagaatcta ctgacaggat ctattgcttc agattttcaa aattcgatag aataagaaat
    14041 tagttagcta gcttaaattt aataaataga aagactgatt gtcagtagtt cgtcataaaa
    14101 tttataaaac ctgaacaacc tttaaagagg atgttcaggt tttattatag tgaaccaaac
    14161 cgcttttgtc atagtcacga gtgtttgcaa ctcatcccgt gcagcaagca cccgattacg
    14221 gttgaggtct gtaaaaccgt caatgggaaa gaagacagcc gttgttgtcg gagtgatttt
    14281 tccgtgttca caaaatcagg cgtttctgaa cagtctattt ttttatctta tttctataat
    14341 tttgattcta ttatgtgttc acaaacccat atcaaaatct gtgttcattt atactattgg
    14401 tataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
    14461 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
    14521 ttgtgaaaaa atttttaccg atcatatgag tggagcaaaa agtaatcgac caggattaga
    14581 aatggcgatt gattttgttc gctctggcga tacacttgtt gtttggcgct tagatcgttt
    14641 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gaggtgttag
    14701 ttttcatagt cttcaagaaa atattacgat ggataaatct agttcgactg gtcagctcat
    14761 gtttcatttg tttgcagcat tcgcagaatt tgaacgcaac ctgattttag aaagatccgc
    14821 agcaggacga gcagcagctc gagcaagagg gcgatttgga ggacgtcctg aaaaattaaa
    14881 agcacaagac ttagaattat taaagactct agttgatagt ggaacaccta ttaaaacgat
    14941 tgccgaacgt tggaatgttt ctcgaacaac gatttatcgt tatttagata aaatggaggg
    15001 aaaagatgga aaaacaaaca ctcactagtt tttcagaaca gcaaagaata gctgcgatga
    15061 aaaaatataa aattattgag ccttatctta ataaacaaaa aacgataaaa gaaatagcta
    15121 ttaaaaataa agcacctact cgtacactct atcgctgggt acaaaaatat gaacatgatg
    15181 gtctagttgg tttaattaga aagactcgaa ctgattttta gcaaattagg gtttcagaag
    15241 aagttcgaca aaaaatagaa gaattggtat tgagatacaa aaaaataagt actaagacat
    15301 tatctcgaaa aattgtttcc tatcatattt agtggaattg ttgtcttttt atttgtaaga
    15361 ggaaatcttt ctttttcgtt tagagatatt ttaatgcctt tcataggttt atttgcttta
    15421 tttggtattt atgaaagtgc gaatcaatca agaaaaagaa aaataaagaa agaaatggaa
    15481 agaatgaaag ccaaagatca tattaagcag taataaaaaa atctagtaag gaggctttct
    15541 tatgagtacg gttattttag ctgaaaaacc aagccaggca ttagcctacg caagtgcttt
    15601 aaaacaaagc accaaaaaag acggttattt tgagatcaaa gacccactat ttacagatga
    15661 aacgtttatc acctttggtt ttgggcattt agtcgaatta gcagaaccag gtcaatataa
    15721 cgaaaagtgg cagaactgga aacttgaatc tttgccgatt tttcctgatc gatacgattt
    15781 tgaagttgca aaagataagg gaaagcagtt taaaattgtt gcagaacttc tcaaaaaggc
    15841 aaatacaatt attgttgcaa cagatagcga cagagaaggt gaaaatatcg cctggtcgat
    15901 tatccataaa gcaaatgcct tttcaaaaga taaaacattt aaaagactat ggatcaatag
    15961 cttagaaaaa gatgtgatcc gaagcggttt tcaaaatttg caaccaggaa tgaattacta
    16021 tcccttttat caagaagcgc aaacacgcca aattgccgat tggttgatcg gcatgaacgc
    16081 aagccctttg tatacgttaa atttacaaca aaaaggcgta caaggtacat tttctttagg
    16141 acgtgtacaa acaccgactt tatatctcat ttatcaacgc caggaagcca tagagaattt
    16201 taaaaaagaa cctttttttg aggtggaagc tagtataaaa gtaaaccaag ggtcatttaa
    16261 gggcgttcta agccccacac agcgatttaa gacccaagag gaacttttat cctttgtttc
    16321 ttcaaatcac gctaaaatag gcaatcaaga gggcagaatc gctgacgtgc aaaccaaaga
    16381 gaaaaaaacg aatagtccta gtctgttttc tttaagtagt ttgcagtcaa aagtcaatca
    16441 gctttataaa gcgacagcga gccaaacttt aaaagctatg caaggactgt atgaagcaaa
    16501 attattgagt tatccaagaa cagatacacc atttattaca gagaacgaat ttgcttattt
    16561 aaaagcgaat tttggcaaat atagcggttt tttaggactt gatcttgaaa tggttcaaac
    16621 agagcctaga aagcgttatg tggacggtag taaggtacag gaacaccacg ccattatccc
    16681 aacaaaacaa gtacctaccg aatctgcatt agcgaaaatg gacgatttac aacgaaaaat
    16741 ttatgcttta gtcgttaaaa cgaccgttgc catgttttta cctgattact tgtatgaaga
    16801 aaccaagata caaacgaaag tagctgattt gctgtttcaa tcgatcggaa agacaccaaa
    16861 acaagaaggt tggaaaattc ttttcaaaca acaaaccaaa gaagaaaaag aggacgttca
    16921 aacgttaccc ttggttatca ttggcgaaca tgccgaggtt gacgttaaga gtgccgaaaa
    16981 agaaacacaa ccaccgaaag cttttacaga gggtacatta ttaactgcta tgaaaaccgc
    17041 taataaaacg gttgatgatg aagaagcaat caagatttta caagaagtcg aggggatcgg
    17101 aacagaagcg acaagagcaa gtattattga agcgttaaaa caaaaagaat atatccaagt
    17161 gattaagaat aagcttgttg taactgaaaa aggaaaatta ttgtgccagg cagttgaaag
    17221 tcagcacctt ttaacgagtg ctgaaatgac ggctaaatgg gaaacgtatt taaaaaaaat
    17281 cggtaaaaga gaaggcaatc aagagaactt tattacgaat atcaaaaaat tcattgttca
    17341 tttactggaa gctgtaccta acgatataga aaaactaaat ttttctgatt accaggaaca
    17401 gaaagaaaaa gaagcagaaa aaagtattgt aggaaaatgt cctaagtgtg gcaacaatat
    17461 tgtattaaaa aaatcgtttt atggttgttc aaattatcct gaatgtaagt ttactttagc
    17521 tgaacatttt agaaagaaaa aactaaccaa aacgaatgta aaagaattac tggagggaaa
    17581 agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag ccctacaatg ccgttgtaaa
    17641 aattggggaa aagggatata ttgattttat atctttctca aaataaacat aaaagtcctt
    17701 taaacagggc ttttatatat taatcactta tcacaaatca caaatcactt atcacaaatc
    17761 acaagtgatt tgtgattgtt gatgataaaa taagaaggaa tagaaagaag tgagtgattg
    17821 tgggaaattt aggcgcacaa aaagaaaaac gaaatgatac accaatcagt gcaaaaaaag
    17881 atataatggg agataagacg gttcgtgttc gtgctgactt gcaccatatc ataaaaatcg
    17941 aaacagcaaa gaatggcgga aacgtaaaag aagttatgga aataagactt agaagcaaac
    18001 ttaagagtgt gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa
    18061 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
    18121 gttatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
    18181 aataaataca ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
    18241 ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg
    18301 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
    18361 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
    18421 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
    18481 gaattagaca gtcatctat
//
LOCUS       DACIWG010000050        17645 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.052, whole genome shotgun sequence.
ACCESSION   DACIWG010000050 DACIWG010000000
VERSION     DACIWG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.052"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            417..785
                     /locus_tag="IYF54_001990"
     CDS             417..785
                     /locus_tag="IYF54_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ9832313.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            965..1906
                     /locus_tag="IYF54_001991"
     CDS             965..1906
                     /locus_tag="IYF54_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ9832314.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1975..2706
                     /locus_tag="IYF54_001992"
     CDS             1975..2706
                     /locus_tag="IYF54_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ9832315.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2850..5030
                     /locus_tag="IYF54_001993"
     CDS             2850..5030
                     /locus_tag="IYF54_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ9832316.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            5050..5532
                     /locus_tag="IYF54_001994"
     CDS             5050..5532
                     /locus_tag="IYF54_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ9832317.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5548..6309
                     /locus_tag="IYF54_001995"
     CDS             5548..6309
                     /locus_tag="IYF54_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ9832318.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6440..7774
                     /locus_tag="IYF54_001996"
     CDS             6440..7774
                     /locus_tag="IYF54_001996"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ9832319.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8081..8566
                     /locus_tag="IYF54_001997"
     CDS             8081..8566
                     /locus_tag="IYF54_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ9832320.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8559..9242
                     /locus_tag="IYF54_001998"
     CDS             8559..9242
                     /locus_tag="IYF54_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ9832321.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9368..12013
                     /gene="polA"
                     /locus_tag="IYF54_001999"
     CDS             9368..12013
                     /gene="polA"
                     /locus_tag="IYF54_001999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ9832322.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12058..12894
                     /gene="mutM"
                     /locus_tag="IYF54_002000"
     CDS             12058..12894
                     /gene="mutM"
                     /locus_tag="IYF54_002000"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ9832323.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12858..13487
                     /locus_tag="IYF54_002001"
     CDS             12858..13487
                     /locus_tag="IYF54_002001"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ9832324.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13539..13871
                     /locus_tag="IYF54_002002"
     CDS             13539..13871
                     /locus_tag="IYF54_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ9832325.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14183..14674
                     /gene="nrdR"
                     /locus_tag="IYF54_002003"
     CDS             14183..14674
                     /gene="nrdR"
                     /locus_tag="IYF54_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ9832326.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14697..16070
                     /locus_tag="IYF54_002004"
     CDS             14697..16070
                     /locus_tag="IYF54_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ9832327.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16070..16996
                     /gene="dnaI"
                     /locus_tag="IYF54_002005"
     CDS             16070..16996
                     /gene="dnaI"
                     /locus_tag="IYF54_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ9832328.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17125..17565
                     /locus_tag="IYF54_002006"
     CDS             17125..17565
                     /locus_tag="IYF54_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ9832329.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         6005 a         3077 c         3877 g         4686 t
ORIGIN      
        1 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
       61 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
      121 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
      181 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
      241 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
      301 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
      361 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg aaacagatgt
      421 ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat gcaacgtttt
      481 ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg cttataattg
      541 aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca tttgttgagt
      601 cccattcacc tggcacagct actagtgccc catctttgat gttttacagt aaagatatct
      661 ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg gtacagcttg
      721 gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg gttacaggta
      781 aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag tctttttaat
      841 tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca caagcaaata
      901 aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa
      961 atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag aaccagagga
     1021 atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc gtattctagt
     1081 agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat
     1141 gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg atccaagcga
     1201 aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct
     1261 agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc aagaatttaa
     1321 agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt atgaatatga
     1381 cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag agcttccaga
     1441 ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct actgtactgg
     1501 tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca aagatgtcgg
     1561 acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag gagaattatg
     1621 ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc aagtagataa
     1681 acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata tcaattgtag
     1741 caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa aacatttagg
     1801 agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag accatggaat
     1861 ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa caatagtttt
     1921 gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat ccacatgaaa
     1981 tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga aaaacttatt
     2041 cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa tgatcgaggt
     2101 cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac aggagccatt
     2161 tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc ggaggactgg
     2221 tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg
     2281 gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag atattcagaa
     2341 ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta tctttttgtc
     2401 catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag agactttatt
     2461 tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat tgtatttgga
     2521 catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg gattcaggat
     2581 cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg ggttgttttt
     2641 gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc ttggcatgga
     2701 atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt ttatttttat
     2761 agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata gaataaagaa
     2821 gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag acgatcattc
     2881 agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact gtacttaatt
     2941 tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa
     3001 gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt gaaaacttag
     3061 aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg actcccgagt
     3121 tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat
     3181 acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt gaaccactcg
     3241 cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa aaatatattg
     3301 acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat gagattattg
     3361 ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat acgtataaca
     3421 aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa
     3481 tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata ttagctacta
     3541 atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa gcagcaattt
     3601 tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct tcattcgtca
     3661 gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag cgagaactgc
     3721 gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc gaaaacctcc
     3781 gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt gatccggctt
     3841 atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag
     3901 ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga ccagctttta
     3961 tccaattaat taataaatat caagtagata tggtagctat cggaaatggt acagccagtc
     4021 gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa acctattacg
     4081 ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca cgaaaagaat
     4141 ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc
     4201 ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag taccaacatg
     4261 acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca gcggttaacc
     4321 aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata tcaggattga
     4381 ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa ttcaccgcac
     4441 gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa gcaatcggat
     4501 tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat cccgaaagtt
     4561 attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag cttggtacag
     4621 aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa agcctatcag
     4681 tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga cgagatatgc
     4741 gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa gacttgaaac
     4801 cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca tttgttgata
     4861 tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc
     4921 atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa caagtcgata
     4981 cgaaaaaagg cagaatcagt ttgacgatgc tttcccctta tgaagagtaa caatgaggag
     5041 taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc attaaaaagc
     5101 ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac aactggcggg
     5161 cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga agagtcagac
     5221 gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct tcatctagct
     5281 ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa aacaggaggg
     5341 ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta tcgatgccaa
     5401 gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga
     5461 agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa tgaagcacaa
     5521 aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac
     5581 tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga ggaactgacg
     5641 gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa agcagcacaa
     5701 atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg gcatgcgact
     5761 agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa
     5821 agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa ggcttttctg
     5881 atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga gattcttcat
     5941 tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact gcaacgcagg
     6001 actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg
     6061 ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc aaaagaactg
     6121 aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat ggaacgtctc
     6181 catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca cattgatata
     6241 tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac ggatttttct
     6301 cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt tgaaattttc
     6361 aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag
     6421 ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg ttggtatcaa
     6481 aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa atgtccaagg
     6541 atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa atatcacgat
     6601 tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag gaaatgcatt
     6661 tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag tgattcgcta
     6721 tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa caggctctca
     6781 cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc gtccaacaag
     6841 ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct tttcatttga
     6901 ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa tcatgaccaa
     6961 tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta cagctttcca
     7021 aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg cttatttacg
     7081 caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg atgatatcca
     7141 agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc atggggatga
     7201 atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa atgctttagg
     7261 tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct
     7321 gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt
     7381 cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg cacgccaaaa
     7441 atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc gtacgattgc
     7501 attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact tatgcgatat
     7561 ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat
     7621 caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag attatcatga
     7681 tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa
     7741 acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg tccttataaa
     7801 aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca acttatgata
     7861 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa aacatgagaa
     7921 gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca cgacctctgt
     7981 aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct acttttggta
     8041 caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag gtaagccgag
     8101 aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct taacagaatc
     8161 tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa atcctttgta
     8221 tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc catcagtgat
     8281 gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag gatcacatgt
     8341 tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac tgacttttca
     8401 gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg tagaagcgga
     8461 gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc
     8521 tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca agtacatcaa
     8581 tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg ggacttgccc
     8641 ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta gcaactttta
     8701 tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg
     8761 ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt attgcctact
     8821 ctcttttgaa tggcataata ttagcggtta ctcttgcata ctacgatatt ggaacagtaa
     8881 cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc ggagtaacaa
     8941 caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc ggagtgatca
     9001 ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg ttcatctccc
     9061 tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc atccgtaata
     9121 tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg gcattgaagc
     9181 tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga cggaacaact
     9241 aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg gtcccgcttt
     9301 ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac agtaaggagt
     9361 taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg cttttcgcgc
     9421 attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa
     9481 tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc aaccgactca
     9541 tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt atgcggagta
     9601 caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat atatacgtga
     9661 tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag cagatgatat
     9721 tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg tgctttcagg
     9781 tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg
     9841 tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg atgggttgac
     9901 gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca atatccccgg
     9961 tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg gctcagtcga
    10021 aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa
    10081 tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt cagcgcctgt
    10141 ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac tcgttccgtt
    10201 ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag aagaagaagt
    10261 aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac
    10321 ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg aaatcgtcgg
    10381 agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg tattcgaaaa
    10441 ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat atgatgcgaa
    10501 acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg attttgatgt
    10561 cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc
    10621 tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca aaggggcgaa
    10681 aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa aagtagctgc
    10741 aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc aagaagatct
    10801 tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa taagcgggat
    10861 caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc gcttaagaga
    10921 gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt caccaaagca
    10981 attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg
    11041 atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa tcgtagagga
    11101 tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa
    11161 agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga cccaaacagg
    11221 acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag atgaagggag
    11281 aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt cttctgacta
    11341 ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt tgaaagctgc
    11401 cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa
    11461 ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt
    11521 gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa aagcagcaca
    11581 gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata tggaaagaat
    11641 cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct
    11701 gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa cagctatcaa
    11761 tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg aattggataa
    11821 acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg atgaacttgt
    11881 cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg aagttatgga
    11941 acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa aaacgtggta
    12001 cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg
    12061 ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt agtaggaaaa
    12121 acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga gacacccatc
    12181 tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg gaagttttta
    12241 atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg aaaatatcaa
    12301 tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt ttttgaggat
    12361 ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa
    12421 ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt gccagatagc
    12481 ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat caagccattg
    12541 ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga agcattatgg
    12601 gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga ggtagaacaa
    12661 ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg taccacgatt
    12721 cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact gcatgtctat
    12781 cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca
    12841 caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg
    12901 ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa gtctttcagt
    12961 ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt gagcctggaa
    13021 cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag cctgatgggc
    13081 aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa cggcaaaaac
    13141 ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc gcctctctaa
    13201 gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt cattatgacc
    13261 actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt cagcgtttga
    13321 tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt caacttccaa
    13381 tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca aaagaaatac
    13441 ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta tctgtgtcaa
    13501 ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta aaaaatcaaa
    13561 aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc
    13621 taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag cagtaccaag
    13681 caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg caagatttga
    13741 ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca gaaagtgacc
    13801 acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca ggaatcgaag
    13861 agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga aggtgttcat
    13921 ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt
    13981 aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc
    14041 atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt
    14101 gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat
    14161 gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt
    14221 aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa
    14281 ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa
    14341 gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc
    14401 agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa
    14461 ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat
    14521 ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca
    14581 gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga
    14641 aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg
    14701 aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc
    14761 tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag
    14821 ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg
    14881 aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg
    14941 caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc
    15001 tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag
    15061 actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta
    15121 cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat
    15181 ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat
    15241 cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat
    15301 tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc
    15361 caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg
    15421 gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc
    15481 aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg
    15541 tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc
    15601 tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa
    15661 aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag
    15721 gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg
    15781 ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg
    15841 ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa
    15901 aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc
    15961 ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga
    16021 ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt
    16081 aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat
    16141 gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga
    16201 attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa
    16261 gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac
    16321 gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca
    16381 aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc
    16441 ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct
    16501 agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt
    16561 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
    16621 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
    16681 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
    16741 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
    16801 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
    16861 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
    16921 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
    16981 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
    17041 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
    17101 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
    17161 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
    17221 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
    17281 gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct tcagttgaaa
    17341 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
    17401 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
    17461 ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
    17521 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
    17581 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
    17641 aattt
//
LOCUS       DACIWG010000051        17020 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.053, whole genome shotgun sequence.
ACCESSION   DACIWG010000051 DACIWG010000000
VERSION     DACIWG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.053"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..2437)
                     /gene="parC"
                     /locus_tag="IYF54_002007"
     CDS             complement(<1..2437)
                     /gene="parC"
                     /locus_tag="IYF54_002007"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ9832330.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEI"
     gene            complement(2449..4494)
                     /gene="parE"
                     /locus_tag="IYF54_002008"
     CDS             complement(2449..4494)
                     /gene="parE"
                     /locus_tag="IYF54_002008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ9832331.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4660..5094)
                     /locus_tag="IYF54_002009"
     CDS             complement(4660..5094)
                     /locus_tag="IYF54_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ9832332.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5221..5859
                     /gene="plsY"
                     /locus_tag="IYF54_002010"
     CDS             5221..5859
                     /gene="plsY"
                     /locus_tag="IYF54_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ9832333.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(5991..6866)
                     /locus_tag="IYF54_002011"
     CDS             complement(5991..6866)
                     /locus_tag="IYF54_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ9832334.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(6948..7739)
                     /gene="codY"
                     /locus_tag="IYF54_002012"
     CDS             complement(6948..7739)
                     /gene="codY"
                     /locus_tag="IYF54_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ9832335.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7757..9157)
                     /gene="hslU"
                     /locus_tag="IYF54_002013"
     CDS             complement(7757..9157)
                     /gene="hslU"
                     /locus_tag="IYF54_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ9832336.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9171..9719)
                     /gene="hslV"
                     /locus_tag="IYF54_002014"
     CDS             complement(9171..9719)
                     /gene="hslV"
                     /locus_tag="IYF54_002014"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ9832337.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9756..10661)
                     /gene="xerC"
                     /locus_tag="IYF54_002015"
     CDS             complement(9756..10661)
                     /gene="xerC"
                     /locus_tag="IYF54_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ9832338.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10859..12235)
                     /gene="trmFO"
                     /locus_tag="IYF54_002016"
     CDS             complement(10859..12235)
                     /gene="trmFO"
                     /locus_tag="IYF54_002016"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ9832339.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12204..14282)
                     /gene="topA"
                     /locus_tag="IYF54_002017"
     CDS             complement(12204..14282)
                     /gene="topA"
                     /locus_tag="IYF54_002017"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ9832340.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14404..15261)
                     /gene="dprA"
                     /locus_tag="IYF54_002018"
     CDS             complement(14404..15261)
                     /gene="dprA"
                     /locus_tag="IYF54_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ9832341.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15317..16084)
                     /locus_tag="IYF54_002019"
     CDS             complement(15317..16084)
                     /locus_tag="IYF54_002019"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ9832342.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16077..16937)
                     /gene="ylqF"
                     /locus_tag="IYF54_002020"
     CDS             complement(16077..16937)
                     /gene="ylqF"
                     /locus_tag="IYF54_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ9832343.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4332 a         3801 c         2959 g         5928 t
ORIGIN      
        1 aaatctcagg agcatcgata acttcgatga cttctccacc ttttttctca tccatcgcaa
       61 atgatccatt cgatgttcga tcacctatag ggaaatcagt tgggtcaatc gttaactgct
      121 gccctttttg attcatcaag gttatttctt ttgatcttcc ttccgacata tagataatac
      181 ggtgcggatt ttttttcagc tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt
      241 ggctgatttc ttgagcaagc atgcgtttaa cagctcctcg ctgagtaacg attacgatcg
      301 gcgtatcacc ttctgctaac acaagcagcc cgttgataat cctatctcca tctttcaaat
      361 tcatagactt cactccagct gcctttgctc caacaacggg aacatctgtt aaaggatagc
      421 gcaaacccat tccttgatgc gttactaaga aaacatctaa gcctggctga tcttctttca
      481 gatacacagc aatcagttca tcttcttccg cctttagctt catacagctg agcggacggc
      541 tcttataggt gcgccatggt tcgaactctg tcattcgtgt ttgcttgatc aatccattct
      601 tgctgatgaa tacaaatgtt ttctctgctt gaattttttg atatgggaaa acagctaaaa
      661 tcgattcgtc tacagacaga ttggagattg tttgagaaat gtgctcacca gcatctttcc
      721 atttcaaatc gggtagttca tggacagggc ggtagatcac atttgcttta tttgtgatca
      781 gaagaacatg gtccaatgta ctcaattcac ctgagtatag caagtagtcg ccttctcgca
      841 tcccgatttc ttctggtttg gaagcactat aagaacgaag gcttgttcgc ttcacatagc
      901 cttctcgcgt cacactgaca attacgtctt cttgtgccac taagacttgc gtatcaattt
      961 tgatttcttc gatttcgtct tcgattgtcg ttagacgagc ggaagaataa ttttttttca
     1021 cttctcgcaa ttcttttttc ataacagaga aaagctcttt gtcatttgaa aggattttat
     1081 tcaactctgt gatttgcgca atcaagcttt cggattcctt ttgaagctct gtgatatccg
     1141 tgttcgtcaa acggtataat tgcaatgtga caattgcttc tgcttgttct tctgtaaatt
     1201 gaaagacgtc gactaaattc tttttggcgt cttttttgtc tttactttca cgaatcgttg
     1261 cgattacttc atccagaatc gataaagctt tcatcaatcc ttctacgata tgctgtcttt
     1321 tttgagcttt ggctaaatcg aattgactac gttttgttat gacttgcttt cgatgctcga
     1381 tataacttct taaaatgtct ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga
     1441 ccatattgaa attgtaattg atctgtagct cagtgttttt gaacaaataa ttcaaaattc
     1501 cttgagcatt ggcgtctttt ttcaattcga cgacaatttg cagacctgtt ctatcacttt
     1561 catctcgtac ttcagctatt ccatcgattt tcttattcaa acggatctca tccatttttt
     1621 tcaccaaggt cgctttattt acttcatatg ggatttctgt gatcacgatt tgctgtttat
     1681 tacccttcat tgcctctatc tttgttttcg aacgaagaat aacttttcct cgtcctgttt
     1741 cataagcttt tttgatttca tcttttcctt gcaagatccc tcctgttgga aagtcaggtc
     1801 caggaatata ttccatcaat ttttcaaggc tcgcttgtgg atgatcgatc atatagattg
     1861 tgccatcgat cacttcagct aagttatgag tcgggatttc tgttgcatat ccagcagata
     1921 tacctgtcga tccattgacg agaaggtttg ggtatcgagc tggaaggact gttggttctt
     1981 tttctgtatc atcaaaattc cacacgagat cgactgtttc tttctcgata tctgctagca
     2041 tctctccact taacttggat aatctggctt cagtataacg catcgcagca ggtggatcac
     2101 cgtccatact cccgttattc ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt
     2161 ggctcatgcg gaccatcgct tcataaatac tgatgtcacc atgaggatga taattcccca
     2221 tgatatttcc gacagatttt gctgatttac ggaatccttt gtcatacgta ttgccatctt
     2281 tgttcatggc aaataatatc cggcgttgca ctggcttcaa tccatcacgg atatcaggca
     2341 gtgcacgttc ttggataata tatttggaat aacgtccgaa gcggtcaccc atcacttctt
     2401 ctaaagttaa ttcttgtact tcttgtcgat tttccatcag gtgacctcct attctacttc
     2461 aaataactga ttgttttcat ttttatctgc tcgttcttca tctaataaat cattagaaac
     2521 tgaaggagag atttcagtat cctctttttt atctaagata ctgccatctt cttcgagcgt
     2581 aaattgcacg tgattttcga tccattttct tcgaggttct actttgtcac ccatcagtgt
     2641 ggttactcga cgttcagctt gcgcagcatc atcgatacgg acgcggatca acgtacggga
     2701 agtcggatcc atcgttgttt cccacaattg ttccgcattc atctctccaa gtcctttata
     2761 tcgctgcagc atatatcctt tgccgacttt tttaatcatt gctgccaatt cgtcatctgt
     2821 ccacgcatat tcaatgactt gcttctttcc ttgtcctttg gatactttat ataaaggcgg
     2881 taacgcgata tatactttcc cagcttcaat cagcggtttc atataacgat aaaagaaagt
     2941 aagcagtaat acttgaatat gggcgccgtc tgtatccgca tcggtcatga tgattacttt
     3001 gtcgtaatta cagtcttcga tagaaaactc aggaccgact cctgcaccaa tcgtatagat
     3061 catcgtattg atttcttcat ttttcaagat atcttgcatt ttagcttttt cggtattgat
     3121 gacttttcct cgtaaaggta gaattgcttg gaactttctg tctcttcctt gtttagcaga
     3181 tccgccagca gaatcccctt cgactaaata caattcattt ttcttagggt ttcgggattg
     3241 tgcgggtgtc aattttcctg aaagaagaga ttcgcctttt ttacgcttct tcccatttcg
     3301 gctttcttct ctggctttac gtgctgcttc tctcgcttct ctggctttga ttgctttacg
     3361 gaccaacatc tggctcattt cgctattttc ctgcaaataa aagcccattt gttcactgat
     3421 gacgttatct actacggtac gagcaactgg cgtaccaagt ttttcttttg tttgcccttc
     3481 aaattgcaga agattttcag gtacacggat agaaagtaca gcagctaacc cttctctgaa
     3541 atcactacct tccagattct tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc
     3601 attataagat ttcgtcatcg ccgctttcat accagcttca tgggttcccc catcttttgt
     3661 ccgaacgtta ttgacaaaag ataaaacatt ttctgaataa ccgtcattgt attgataagc
     3721 gacctctact tcgatacctt ctttttctcc tgaaaaataa acaactggtg tcaatgtatc
     3781 tttttcttca ttcagataat caacaaattc tttgattcct tcttcgtaat gaaaaatttc
     3841 cttgactggt tcttctccac gaagatcaga aagagagatt ttaacgcctt ttagaagaaa
     3901 ggcagattct cttaaccgtt cggctaaaat ttcatatgaa aatttcgttg tactgaatat
     3961 tgtatcatct ggtaaaaaat ggacagtcgt cccgtttgct ttttttgttt ttcctatctt
     4021 tttcaaagta ccgtcaggtt taccgccgtt tttaaatttc tgctgatact ctacgccatc
     4081 tctaacgatc gttacaatta gccatttcga taaggcattt accacactgg ctcctacacc
     4141 gtgcagtcca ccagaagttt tgtaccctcc ttgaccaaac tttccgccag catgcagcac
     4201 agtaaaaatc acttcaactg ttggtattcc ggaagcatgc attcctacag gcatcccacg
     4261 tccgctatct gcaacagtta tactattgtc ttcatggatg gtcacatcaa tctcactgcc
     4321 ataacctgat aatgcttcgt caactgcatt atcgacgatt tcatagacta aatgatgcaa
     4381 gcctcggctg tcagtggatc caatgtacat ccctggtctt tttctaactg cctctaatcc
     4441 ttccaatact tggatggacg agtcattgta ttcgttgttt actttttttg ccaaggcaaa
     4501 cgctccttca ataatacttt gcggcaaaac cgcaccacga ttatcatact agaaaaggtc
     4561 gtgaaaaaaa agagggtaga cgagaaatca attttaatta tctcctaacc gctttttcga
     4621 cattcccctc tatcgatcat ctcttctttg tggtcatttt tatttttggt tttctttcaa
     4681 cttaccaagt tcaatcttaa tacaacgatc cattacgata tctgtacgtc ctgcttttct
     4741 tagcatctca gctgcttctt tgttttcaat ccctaattgt gcccagaaca ctttagcatc
     4801 agttttcaaa aaatcacctg ctatttcagg taaaaattca ctccgtctaa atacatctac
     4861 gatatcgatt ggatcatcga tttctgtcaa attcgcatag actttctcgc ctagaatttc
     4921 ttcaccagcc aataatggat ttactggtat gattttatat ccattgtctt gcaatacctg
     4981 tgcaaccatg taacttgttc gttctggttt attgctcaag cctacgacgg caatattttt
     5041 tgcttcttcc aaatagttga aaatcgtttc ttgtgttgga ttttttacag acatttctgc
     5101 caccccttgc ttttcttttt attccatcta ttatacttgt atctttcatt ttttcaattg
     5161 ttcctgttac ctattcatgc taaaataaag gatagttttc aacttgaaag agggtaaaaa
     5221 atgaaattga tcttattact tattttagca tatcttttag gttctattcc ttccggtgtc
     5281 tggattggca aattgttttt caaaaaagat atccgacaac acgggagcgg taatactgga
     5341 acgaccaata catttcgtgt attaggcaaa acagccggga ttgttgtcct gcttatggat
     5401 attttaaaag gtacattggc tacttgtttg ccgatgatct tccacctttc tgggattaac
     5461 cctctttggt ttggggtatg cgccattctt ggccatacat ttccgatttt tgctgggttc
     5521 aaaggaggaa aagctgttgc gacaagtgca ggaatgcttt taggatttaa tcctgtattt
     5581 tttgtttatt cttgtatcat ctttatcgtt agtttatttt gtacaagtat ggtgagtttg
     5641 agcagtatga tcagtgcagt attgatcact ttatctacga tcgtccttcc ttatgtagct
     5701 cctgtgattt tggctaagcc caattggtta ctgacgatca ttgccttttt agtttcttcc
     5761 tttatctttt atcggcataa agacaatatc aagcgtatca gaaaccatac tgaaagtcga
     5821 atcccgtttg gtttgaatag cagcaagaaa aaatcctgat actttttcat tccaaagaga
     5881 taaaacggga gtgtgacaaa tcgatgtcac actcccttat tacttacagg atagcttttt
     5941 tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact
     6001 gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt
     6061 tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa
     6121 acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag
     6181 cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt
     6241 gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt
     6301 gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat
     6361 cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga
     6421 ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt
     6481 gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca
     6541 aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt
     6601 tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt
     6661 tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat
     6721 aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca
     6781 tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc
     6841 tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa
     6901 tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt
     6961 tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct
     7021 aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca
     7081 gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca
     7141 tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt
     7201 gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa
     7261 aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca
     7321 aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc
     7381 ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta
     7441 atatcactag taattgggat attagcttca gttttcatca actgtgccac tgtatcagta
     7501 taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg
     7561 tgacgttcat tataacctag taatactccg tcacttccta tgatataggt gttgctgtca
     7621 agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa
     7681 gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt
     7741 gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc
     7801 caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg
     7861 cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga
     7921 tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt
     7981 ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag
     8041 cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta
     8101 cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga
     8161 aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta
     8221 cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc
     8281 cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt
     8341 cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt
     8401 cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct
     8461 tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt
     8521 tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca
     8581 gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca
     8641 cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt
     8701 caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca
     8761 aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta
     8821 cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat
     8881 aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg
     8941 ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt
     9001 ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca
     9061 aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac
     9121 ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct
     9181 tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt
     9241 tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca
     9301 gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact
     9361 tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca
     9421 agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt
     9481 agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct
     9541 gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt
     9601 gcagtacctt tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca
     9661 aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg
     9721 tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
     9781 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
     9841 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
     9901 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
     9961 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
    10021 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
    10081 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
    10141 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
    10201 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
    10261 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
    10321 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
    10381 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
    10441 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
    10501 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
    10561 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
    10621 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
    10681 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
    10741 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
    10801 aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt
    10861 actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag
    10921 ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag
    10981 gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt
    11041 agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg
    11101 ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt
    11161 ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg
    11221 gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga
    11281 attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca
    11341 agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct
    11401 ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag
    11461 gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt
    11521 caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct
    11581 catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt
    11641 tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg
    11701 gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg
    11761 ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag
    11821 taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt
    11881 cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg
    11941 ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat
    12001 tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt
    12061 gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt
    12121 gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt
    12181 tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca
    12241 cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga
    12301 caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa
    12361 ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact
    12421 gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta
    12481 ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca
    12541 tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca
    12601 ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc
    12661 gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg
    12721 atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca
    12781 aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca
    12841 taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta
    12901 aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca
    12961 tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct
    13021 tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg
    13081 acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta
    13141 gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt
    13201 ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt
    13261 gcgccttgag catttttgat ctttcggtga ccatgtctag aaaattcttt tccatacgta
    13321 tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta
    13381 cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat
    13441 tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt
    13501 tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact
    13561 tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca
    13621 tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga
    13681 gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct
    13741 ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct
    13801 ttcttgacct tacgccagag aataggactg atcgaataac caacgattct gtctaaggta
    13861 cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct
    13921 gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc
    13981 aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt
    14041 gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct
    14101 tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat acccatttta
    14161 cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga
    14221 tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc
    14281 attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa
    14341 atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg
    14401 gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc
    14461 ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa
    14521 aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt
    14581 ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa
    14641 acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc
    14701 aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat
    14761 gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc
    14821 tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata
    14881 aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca
    14941 agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag
    15001 cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt
    15061 gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat
    15121 tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca
    15181 gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa
    15241 attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg
    15301 taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga
    15361 ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat
    15421 tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat
    15481 atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta
    15541 ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta
    15601 caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca taaatattca
    15661 catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa
    15721 cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta
    15781 atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc
    15841 ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt
    15901 caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt
    15961 caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg
    16021 taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag
    16081 tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct
    16141 tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt
    16201 tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa
    16261 ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc
    16321 gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg
    16381 ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac
    16441 tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt
    16501 gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct
    16561 aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt
    16621 tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc
    16681 gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt
    16741 tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc
    16801 tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg
    16861 atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga
    16921 aaccattgga tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta
    16981 cttgtttttt tctttttcaa tataaattca cttttttatt
//
LOCUS       DACIWG010000052        16943 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.054, whole genome shotgun sequence.
ACCESSION   DACIWG010000052 DACIWG010000000
VERSION     DACIWG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.054"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(258..1763)
                     /locus_tag="IYF54_002021"
     CDS             complement(258..1763)
                     /locus_tag="IYF54_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9832344.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IYF54_002022"
     CDS             complement(1760..2446)
                     /locus_tag="IYF54_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832345.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IYF54_002023"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IYF54_002023"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ9832346.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IYF54_002024"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IYF54_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ9832347.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IYF54_002025"
     CDS             complement(4467..5036)
                     /locus_tag="IYF54_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ9832348.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IYF54_002026"
     CDS             5222..6124
                     /locus_tag="IYF54_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9832349.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IYF54_002027"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IYF54_002027"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ9832350.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IYF54_002028"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IYF54_002028"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ9832351.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IYF54_002029"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IYF54_002029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ9832352.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IYF54_002030"
     CDS             12692..12877
                     /locus_tag="IYF54_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ9832353.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IYF54_002031"
     CDS             complement(12939..14135)
                     /locus_tag="IYF54_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ9832354.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IYF54_002032"
     CDS             complement(14305..15741)
                     /locus_tag="IYF54_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ9832355.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IYF54_002033"
     CDS             16034..16843
                     /locus_tag="IYF54_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832356.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4969 a         3550 c         2961 g         5463 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa tttttgggct ctataattta tgggactgat gaatcagaat
    16921 tgattcttca gccccatttt cat
//
LOCUS       DACIWG010000053        16537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.055, whole genome shotgun sequence.
ACCESSION   DACIWG010000053 DACIWG010000000
VERSION     DACIWG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.055"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYF54_002034"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYF54_002034"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ9832357.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IYF54_002035"
     CDS             complement(1174..1869)
                     /locus_tag="IYF54_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ9832358.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IYF54_002036"
     CDS             complement(1882..2598)
                     /locus_tag="IYF54_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ9832359.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IYF54_002037"
     CDS             2928..3533
                     /locus_tag="IYF54_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ9832360.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IYF54_002038"
     CDS             3858..4181
                     /locus_tag="IYF54_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ9832361.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IYF54_002039"
     CDS             4363..5733
                     /locus_tag="IYF54_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ9832362.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IYF54_002040"
     CDS             complement(5863..7164)
                     /locus_tag="IYF54_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ9832363.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IYF54_002041"
     CDS             complement(7394..9385)
                     /locus_tag="IYF54_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ9832364.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IYF54_002042"
     CDS             complement(9529..9795)
                     /locus_tag="IYF54_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9832365.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IYF54_002043"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IYF54_002043"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ9832366.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IYF54_002044"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IYF54_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ9832367.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IYF54_002045"
     CDS             13139..13702
                     /locus_tag="IYF54_002045"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ9832368.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IYF54_002046"
     CDS             complement(13955..14476)
                     /locus_tag="IYF54_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ9832369.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IYF54_002047"
     CDS             complement(14479..15321)
                     /locus_tag="IYF54_002047"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ9832370.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IYF54_002048"
     CDS             complement(15314..16039)
                     /locus_tag="IYF54_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ9832371.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IYF54_002049"
     CDS             16215..16511
                     /locus_tag="IYF54_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832372.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4652 a         3458 c         3062 g         5365 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttataacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgat acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacagg
//
LOCUS       DACIWG010000054        16282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.056, whole genome shotgun sequence.
ACCESSION   DACIWG010000054 DACIWG010000000
VERSION     DACIWG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.056"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            807..1007
                     /locus_tag="IYF54_002050"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IYF54_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IYF54_002051"
     CDS             1494..1673
                     /locus_tag="IYF54_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832373.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IYF54_002052"
     CDS             complement(1719..2324)
                     /locus_tag="IYF54_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832374.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="IYF54_002053"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="IYF54_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3006..3233
                     /locus_tag="IYF54_002054"
                     /pseudo
     CDS             3006..3233
                     /locus_tag="IYF54_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3359..4330
                     /locus_tag="IYF54_002055"
     CDS             3359..4330
                     /locus_tag="IYF54_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9832375.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="IYF54_002056"
     CDS             4470..5783
                     /locus_tag="IYF54_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ9832376.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="IYF54_002057"
     CDS             5826..6692
                     /locus_tag="IYF54_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9832377.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="IYF54_002058"
     CDS             6689..7537
                     /locus_tag="IYF54_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9832378.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="IYF54_002059"
     CDS             7551..9023
                     /locus_tag="IYF54_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ9832379.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..9912
                     /locus_tag="IYF54_002060"
     CDS             9040..9912
                     /locus_tag="IYF54_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9832380.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(10146..11527)
                     /locus_tag="IYF54_002061"
                     /pseudo
     CDS             complement(10146..11527)
                     /locus_tag="IYF54_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11989..12270
                     /locus_tag="IYF54_002062"
                     /pseudo
     CDS             11989..12270
                     /locus_tag="IYF54_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12344..13024
                     /locus_tag="IYF54_002063"
     CDS             12344..13024
                     /locus_tag="IYF54_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ9832381.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVSH
                     ATIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            13750..13845
                     /locus_tag="IYF54_002064"
     CDS             13750..13845
                     /locus_tag="IYF54_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ9832382.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(14147..14392)
                     /locus_tag="IYF54_002065"
     CDS             complement(14147..14392)
                     /locus_tag="IYF54_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832383.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            14737..15021
                     /locus_tag="IYF54_002066"
     CDS             14737..15021
                     /locus_tag="IYF54_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832384.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            15024..15440
                     /locus_tag="IYF54_002067"
     CDS             15024..15440
                     /locus_tag="IYF54_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832385.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(15577..16254)
                     /locus_tag="IYF54_002068"
     CDS             complement(15577..16254)
                     /locus_tag="IYF54_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9832386.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         5170 a         2822 c         3123 g         5167 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtgaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaactttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaacgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgttttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggcaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 agaaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttctaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaatacctt tagtcattga cttcttcaaa
     9181 caataccaag cagatttagc agggattggg attggctcat ttgggcctat cgatatccat
     9241 cgcgattccg ccacttatgg gtacattacc tcgaccccga aagtggcatg gcaaaatttt
     9301 gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc
     9361 aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt
     9421 tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa
     9481 gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt
     9541 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
     9601 gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc
     9661 gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg
     9721 atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca
     9781 tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc
     9841 ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc
     9901 ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac
     9961 tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa
    10021 gatcaagcct tgaatgagat aagttcttgc tcgaaagtta tggaaatttc gatagccgta
    10081 ggccacttgt attgtgcaat agttttttac aaaaaaaggg ggtgcggata tttttttgga
    10141 cctttttaac aaatatatct ttgcttcttc cgatattgaa gagggcttag ccctcctaaa
    10201 gataacttaa ttcgttgatt attataccaa tgaatataat catccaattt gtttataaac
    10261 tgattgattg ttgtattctt ccagtcacgt tgataaaaca tctcgttttt taatcgtcca
    10321 aagaaacctt cgcacgctga attatctggt gaataaccct ttttagacat agagtgaagc
    10381 aagttggctt tgttccaata gtccaactaa caatagcacc atcgtaacaa tcgacaagtg
    10441 gtgataagta gattttccct gcgggaattc taaattcagt aatatctgtt aaccattttt
    10501 catttggctt ctttgcttta aaattccgat ttaataaatt ggggtgtgca ggagaaattt
    10561 ccccagcata cgaagaatac ttttttcgtc tagaaaattt aacaaataga ttttcctcag
    10621 tcatcaatct acgtacaact ttttcagaaa gctttttacc catatttttt aaagcttggt
    10681 gaattctacg gtaaccatac gcacagaagt tatgattgaa aatcgtgata attaattgac
    10741 gttctttgta atacttatct ggtttctcaa gtgcttgttt ttgataaaaa tagctgcttt
    10801 taggcaactg gagctgttga agaagctctt tcaaggtgaa ctgatttcgt aaggcatcaa
    10861 tcaatatcgt tttttcttga tttgatagac aaagaagatt gacgcctttt tcttttttta
    10921 gcagttcagc cgctccttcg agtaatgttt tttcgagctg taattggtgt acttgttttt
    10981 gtaagtcttc tacttccttt aaaagtagct ctttttgttt ttcctgattt tttaaagtca
    11041 tacgcaatgg aaaatccttt ccaagaaccg tgtttttcca ttgatataga gtaactctac
    11101 taattccttc agattctgct aaatttttcc tattagtttg cggtttatag aattttttca
    11161 acacagcttc tttttgatct tgtgttaact ttaccgcact ttttcggagt tttctagctc
    11221 ctggtgcaag ttcttcacac cgctgccgca aggactctct attaggataa ccaagcatgc
    11281 gaatggttcg tgcataacat tgaccatatt caaaataatg attcaccgct attcgttttt
    11341 gttcttcaga atactttgat ttttttcggt gctcctgatg gagctctccg gattccaaat
    11401 actctttata ccatttccgt agtgcaccaa tcgacggata ccctaattct cgaatggttg
    11461 cagcataaga gcaatcgtat tttaaaaata actgaattgc ccttacacgc tctttgtggg
    11521 aaaacacacg gacattcctc ctaagtttat tttttcgtcc aggattatgt ccgcaccccc
    11581 ttgctttctg atgacttatt ctggttcttt gagtgtcaat gcatagatgt gatgtagcta
    11641 gttagaaaaa aattttgaat actctttttt tctttcagac tcctttttaa gtaagatttt
    11701 gaagtgtgag ttacggggtc agaaacttaa aagtttttga tcctttattt tcaagtctga
    11761 tttggcagct tttcggcagc tcatttattt atggtcagaa atagatgctg aaattttaat
    11821 ctattagcaa atagtccaga acgtatgaag aggagtcgga tatcatttgt cattaacacc
    11881 ataaatattt ataaaattcg tgttcaccta caattaaatt taatatataa tattcgttgt
    11941 aagggcttat aattttttgt taatgaacaa gataggaaga aacatttaat ggacagtttt
    12001 cagagattgt cactagtaaa tcaatttgag ttattggcta aatttgagga ccaaaacaga
    12061 tcttattatg aaagaaaaat cgaaatattg agagaaggtt acgagtatca ttatgatgat
    12121 gaaatttgga gtgatctttc tgagccattt ccgaaagaag attctcgatt tgtattagat
    12181 gtattgaaca tgtatcgaga tatcaatttt agtaaatcaa agctaaactt agatgaaaac
    12241 caaattacta attcatacta tactcatttc ggttctgttg caaagttttc caaaaaatct
    12301 attttagtgt aaaattgaga aaaaagacag agagggcaga gtaatgaatc attttaaagg
    12361 caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc gttacaatct
    12421 aagctatcgt gaagttcagg aattgttata tgatcgtggc attaacgttt ctcatgcgac
    12481 gatttatcgt tgggtgcaag aatatggcaa actactctat caaatttgga aaaagaaaaa
    12541 taaaaaatcc ttttattcat ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg
    12601 gcattatttg tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa
    12661 aaaacgggac acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga
    12721 accgaaggtt gtagtcacag ataaagcccc ctctattaca agtgccttta agaaactaaa
    12781 agaatacggc ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat
    12841 tgaacaagac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
    12901 ctctaccacg attaaaggca tggaagccat tcgaggatta tataagaaaa cccgaaaaga
    12961 aggcactctc ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc
    13021 ttaaatcata gataccgtaa gggattttat tctttattta aaactttgca acagaaccat
    13081 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
    13141 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
    13201 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
    13261 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
    13321 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
    13381 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
    13441 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
    13501 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
    13561 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
    13621 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
    13681 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
    13741 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
    13801 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
    13861 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
    13921 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
    13981 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
    14041 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
    14101 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
    14161 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
    14221 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
    14281 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
    14341 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
    14401 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
    14461 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
    14521 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
    14581 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
    14641 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
    14701 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
    14761 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
    14821 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
    14881 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
    14941 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
    15001 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
    15061 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
    15121 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
    15181 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
    15241 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
    15301 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
    15361 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
    15421 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
    15481 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
    15541 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
    15601 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
    15661 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
    15721 tcgtaatgca ggatttaata ctttttgatc aatgttaccc atttgatagt attctgggat
    15781 atccattaag ctcctaaact cttcaatacg aactacataa taaccagtag aacggtactg
    15841 cataagcagt cggaatagag tttttgcata cgtagaacgc aacgacgtga aagcagataa
    15901 ttcaaatcta ctaaattcag ctgttaaacc attaataata tattctaggt ccggattaac
    15961 actgacttca acaaactttt ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta
    16021 gcgaatagaa tcctcatctt cagtacggta agtaagatta agcatatctt tatagagctt
    16081 ttctaaatca ccgacaaagg cttttgtagc agtcatctta taatcactta actcttttaa
    16141 ttcctcgaaa gaaaatctaa cttttttcaa accacgatct ttcatacgag cgcataatgc
    16201 aaaaaataaa tccatctgct tagcattgaa atttttcagc ggtactaaat tcaattcatt
    16261 ttgatatttg actgttaatt cc
//
LOCUS       DACIWG010000055        15963 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.058, whole genome shotgun sequence.
ACCESSION   DACIWG010000055 DACIWG010000000
VERSION     DACIWG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.058"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..450)
                     /locus_tag="IYF54_002069"
     CDS             complement(<1..450)
                     /locus_tag="IYF54_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9832387.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IYF54_002070"
     CDS             674..1465
                     /locus_tag="IYF54_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9832388.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IYF54_002071"
     CDS             1431..2384
                     /locus_tag="IYF54_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ9832389.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IYF54_002072"
     CDS             complement(2782..3756)
                     /locus_tag="IYF54_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832390.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IYF54_002073"
     CDS             3871..4305
                     /locus_tag="IYF54_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9832391.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IYF54_002074"
     CDS             complement(4430..5416)
                     /locus_tag="IYF54_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ9832392.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IYF54_002075"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IYF54_002075"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ9832393.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IYF54_002076"
     CDS             complement(6546..8468)
                     /locus_tag="IYF54_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ9832394.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IYF54_002077"
     CDS             complement(8555..10495)
                     /locus_tag="IYF54_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ9832395.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="IYF54_002078"
     CDS             complement(10783..11523)
                     /locus_tag="IYF54_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ9832396.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="IYF54_002079"
     CDS             complement(11536..12627)
                     /locus_tag="IYF54_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ9832397.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="IYF54_002080"
     CDS             complement(12617..13723)
                     /locus_tag="IYF54_002080"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ9832398.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14419)
                     /locus_tag="IYF54_002081"
     CDS             complement(13742..14419)
                     /locus_tag="IYF54_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ9832399.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14430..15209)
                     /locus_tag="IYF54_002082"
     CDS             complement(14430..15209)
                     /locus_tag="IYF54_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ9832400.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15238..15588)
                     /locus_tag="IYF54_002083"
     CDS             complement(15238..15588)
                     /locus_tag="IYF54_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ9832401.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15604..15960)
                     /locus_tag="IYF54_002084"
     CDS             complement(15604..15960)
                     /locus_tag="IYF54_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832402.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4737 a         3344 c         2690 g         5192 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag aggtagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccgggaag attgctccta aaatcattgc tcctaaaata gcacctccag
    13801 caattggttt tttccaagca tagaatccta gtgctcctaa tgtagcgcca atgccgattg
    13861 gaatagatgc tgcacacgcg cttaatataa ttaatggacc caaaaaacgt ccagatgaat
    13921 ttcctgcccc catcatgacg tcagcaccaa aagtggaatt tgattgattg atcgtaaatt
    13981 tacgaatcaa gacaatgatg ccaccaacaa tcaaacctag aaccaaacca gtcaataaag
    14041 agagtgcaaa attatcgatt ggtgccgcta ttttcatact taagagaatt gctggtaccc
    14101 caattccgat cccagttagg atcgaaccgc ctaagtccaa gatcccaact aaagagcctt
    14161 ctaaaattct ggcaaataag aaactagcac caaacgctgc cgctgcgcca taggatccgc
    14221 catccagccc ggcttttaac atcgccacga tcgaaacttc attgaatgca cccacaccat
    14281 acatcacata catgtgtgtt cctgcaaata cagcagcgga cataattcct acgattagcg
    14341 gaaaagacca gtctgcaaac caaaagctct tttgccgctt tgcttctaat acttttgctt
    14401 gttcttctgt tgtctccatg attctcctcc tatttcacac taaagatgtt gtgcaagtta
    14461 tcaagccaat taggcacttg aagctggaag ctcttgatca tttccatatc aaatccacgg
    14521 aaaaatccac tgaaaataaa taataaaaca atagcgatca tcatgctctt cgtgacgcga
    14581 ttccaaccgc tctcatcgac gcctttacca atcaagattc ctagaacaac accaggtacc
    14641 gcatttccca taatcaattg cgcgatacca ccaaaaagag tgccccaaaa accagttcgt
    14701 ttccctgcgt ctaaagctgc caaccaaaaa ataactggca ttactgtatt gatcagaatc
    14761 gtcgctgctg gtaccaaaac ggaaaccgcc gtcacttgta acgcctctgg aattgctgca
    14821 gctgttgtgt ttaagaaggc taccaccact gccccgataa ccgctccaat gatccccatt
    14881 tttttcggat catgcatggt ctgggtgaca tctttgtttt ttgctagaag tgctgccgct
    14941 gcccaattgg gaatgattcg atgcgtcaca tcttgtgtga atgcaccagc accaactgaa
    15001 gaagcccatg cattaaagaa aaagcctaaa ccaaatgaaa aatgagaagc tggatcaccc
    15061 atgcaggcat tcatttcgcc taatgttcta aaagctccta aaccttgtgt attaggtgca
    15121 tgaaacattc gagctgcccc gacagatgct gcaaaaccaa gtaatccccc gataatgacc
    15181 gatttgacta gaatgattac gaaatccacg ttattctttc ccccttatga ttcaaaatta
    15241 gattttttta gttacaaatg gaatggcaat cccatttgga tcttcagtca tttgctggct
    15301 gaacgatatg tctgccaatt ttatttctgt tacagccaca gtaacagaga gttcgacttc
    15361 atagctaata cgttgccgtg ggaagaagaa aaatagaaag cgttctgtgt atttgttttc
    15421 gaccgccttt aaaatatcca cattgactgg ttcgattcgc aataatacgt ttgattgttc
    15481 cattattttg ttttgaattt ctgctaacgc tgatgcaaaa gcatgttgct ttgtttcccc
    15541 tttaccggag acgataactt tttgctgaat tgtcttttga acgctcattt catgtccctc
    15601 tttctatttt ccatgctttt tgataaattc ttctgtcaga cgtttcccta attcctcttg
    15661 atccataaag ccgaaaccta gaacagtttt gccatccttg atagctgtga tcccttcttc
    15721 aatcgaacgc ataccatgtc gttctggata gccatatttt gttgctgctg ttaatgcacc
    15781 tgcaccacca ctaccacaaa aagagatacc catatctgca tgctcttgtt gcatcacatc
    15841 gcccaaacgc atatctgccc ccattcctgg tacgacaact gcttttccac cagctgcttc
    15901 tacccctttg gccacatttt gtcctttacc catacggtct gcaataacta ctttaatcat
    15961 gat
//
LOCUS       DACIWG010000056        15801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.059, whole genome shotgun sequence.
ACCESSION   DACIWG010000056 DACIWG010000000
VERSION     DACIWG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.059"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            549..1082
                     /locus_tag="IYF54_002085"
     CDS             549..1082
                     /locus_tag="IYF54_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ9832403.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1572..2096)
                     /locus_tag="IYF54_002086"
     CDS             complement(1572..2096)
                     /locus_tag="IYF54_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832404.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(2089..2562)
                     /gene="tsaE"
                     /locus_tag="IYF54_002087"
     CDS             complement(2089..2562)
                     /gene="tsaE"
                     /locus_tag="IYF54_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ9832405.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2685..3665)
                     /gene="pta"
                     /locus_tag="IYF54_002088"
     CDS             complement(2685..3665)
                     /gene="pta"
                     /locus_tag="IYF54_002088"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ9832406.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3818..4498)
                     /locus_tag="IYF54_002089"
     CDS             complement(3818..4498)
                     /locus_tag="IYF54_002089"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ9832407.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4624..5493
                     /locus_tag="IYF54_002090"
     CDS             4624..5493
                     /locus_tag="IYF54_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832408.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5519..6034
                     /locus_tag="IYF54_002091"
     CDS             5519..6034
                     /locus_tag="IYF54_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832409.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(6081..6644)
                     /locus_tag="IYF54_002092"
     CDS             complement(6081..6644)
                     /locus_tag="IYF54_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832410.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6699..6878)
                     /locus_tag="IYF54_002093"
     CDS             complement(6699..6878)
                     /locus_tag="IYF54_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832411.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6955..7464)
                     /locus_tag="IYF54_002094"
     CDS             complement(6955..7464)
                     /locus_tag="IYF54_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832412.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7560..7940)
                     /locus_tag="IYF54_002095"
     CDS             complement(7560..7940)
                     /locus_tag="IYF54_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ9832413.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8270..9019
                     /locus_tag="IYF54_002096"
     CDS             8270..9019
                     /locus_tag="IYF54_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ9832414.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9203..10480)
                     /locus_tag="IYF54_002097"
     CDS             complement(9203..10480)
                     /locus_tag="IYF54_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832415.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10559..11203)
                     /gene="pcp"
                     /locus_tag="IYF54_002098"
     CDS             complement(10559..11203)
                     /gene="pcp"
                     /locus_tag="IYF54_002098"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ9832416.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11402..12346)
                     /locus_tag="IYF54_002099"
     CDS             complement(11402..12346)
                     /locus_tag="IYF54_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ9832417.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12348..13034)
                     /locus_tag="IYF54_002100"
     CDS             complement(12348..13034)
                     /locus_tag="IYF54_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ9832418.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13507..13827
                     /locus_tag="IYF54_002101"
     CDS             13507..13827
                     /locus_tag="IYF54_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ9832419.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13988..14893
                     /locus_tag="IYF54_002102"
     CDS             13988..14893
                     /locus_tag="IYF54_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9832420.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14890..15663
                     /locus_tag="IYF54_002103"
     CDS             14890..15663
                     /locus_tag="IYF54_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9832421.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT         4625 a         3207 c         2830 g         5139 t
ORIGIN      
        1 cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg
       61 tgaaaaagct gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt
      121 tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag
      181 ttgtgaaatc agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat
      241 attttgctat tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
      301 agttgtgaaa aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca
      361 caactttgca taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc
      421 acaaggaatg agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt
      481 tttttgttaa atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt
      541 ttacagaaat gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg
      601 cttgttctct tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat
      661 tgatacagcc tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag
      721 aagacgtacg caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc
      781 aaaaaaatcg tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat
      841 gcttagatta ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta
      901 gccgtcgcct gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga
      961 acatccaatt agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc
     1021 tgctttatca agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt
     1081 aaaagaatac ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac
     1141 aaggagcaat ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag
     1201 gagctggcag gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt
     1261 aataagaaca gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc
     1321 gcagatgcaa gcttttggac accgtttacc aagaggttgt gaaatcagcg ttttgacctg
     1381 agtattagag aaacaaacgg caaaatagtt cctcatattt tgccgttttt tgagctaata
     1441 ccgaaggtca gctgattgaa caccgtttat tcaaaaaaaa tggactgcga cacgactttt
     1501 gtcacagtcc cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt ctcactcttt
     1561 tcctatttct tctattcaat caaataactc attttatata catctaaaaa ttcacctaga
     1621 tctgttcttg cccctctagg catgaccgct tcgatttgga agcccatttt tcggtaaagg
     1681 tgtacggctc tttcattacg aacttgaacg tcaagctcca atcgaaaaag tacacccatt
     1741 tccttcgccc aactgatgat ttcttctagc ataagagtgc ctagtcccat tccccaatat
     1801 tcctgcagaa tactgatacc gacttctccc acgtgagaca aacgaaattg actgtctgcc
     1861 ttgacagatg ctgttccaat aatcgtccct tcatagattg caagcagcaa taaattatta
     1921 ttgctttcgt atatataatc caactcttct tctaaggttt ctggtggtaa caggagttct
     1981 gcttcatcca aaactaaaaa ttctgtctcc ttattaactt gtcccatcat caaaagaatg
     2041 tcttttgcat ctcctggcac tgcttcccgc aagatgatcc caacttcttc actcatgatt
     2101 ttcagcccat ttttctagaa ttcgctgttt gaaggtttca ctttgctccc cgtatgagat
     2161 tagtttcaca taccgctttt ctaaatctgt atcacttttt tctaaaatca gttccaaata
     2221 atcctccggc agtgcttctt ctaatagatt tccccattca acaacggaaa gcccatcacc
     2281 ttcaaaatat tcatctaacc ctaaatctgc tccgctagct gctacccgat aaatatccat
     2341 atgatacaac ggaatccgac cttgactata ttctctaata atcgtgtatg tcggactttt
     2401 gatcatttgc gttatcccta atccttgggc aattcctttc gtcaacgttg ttttacccgc
     2461 acctagatca ccagtcaata tcagattgtc acctggcatt gcggaatgac caattgcctc
     2521 tgcgaattgt tctgtcattg ttaatcctga tagttcgatc atttttcatc ttctcccgct
     2581 cttctttctt tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag
     2641 tcaagaacag cagctgctgt ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt
     2701 tgtgctgctg tgataatcga taatttatat acatcttctt catttgctcc acgagacaaa
     2761 tcagaaaccg gtttgttcaa gccttgtaga attggtccga ttgcttcaaa gttaccgaaa
     2821 cgctgagcaa ttttatagcc gatatttcct gactgcaatt ctggaaatac aaagactgtt
     2881 gcttgaccag ctacttctga atctggcgct ttcagttgtg caactgaagg aacataagaa
     2941 gcatcaaatt gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt agcgatttta
     3001 gttgcttcaa ctactttgtc tacttctggc gatttcgctg agccttttgt cgagaagctc
     3061 atcattgcta cttttggttc gatatcgaac aattctgctg tttttgcaga atctacggcg
     3121 atttcagcca attcttgcgc agttggattc acgttgatcg cacaatccga aaagacatat
     3181 ttttcttgat ctcttccacg taccatgatc atcgctccgc ttgtccgact gatgccaggc
     3241 tttgttttga tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca
     3301 gagaccattc cgtctgcaat ccccatatac gtaagcatcg taccaaagta attcacatct
     3361 tttaaaatct tttctgcttg ttctttggat gcctttccat ttctacgttc aacaaaagta
     3421 tccaccattt catcccactt atcgtaatta tcagggctga tgatcgtaaa gctagatgtt
     3481 ttgattcctc tggcatgcgc tgcatttctt atttcttctt cgttccctac taaaatgggt
     3541 tccacaagtt cttctgcttt caaccgggca gctgcgccta aaatacgagc atcagtagct
     3601 tccgggaaaa cgattttgat cccacggcga acaattttaa atcttaaact atcaaacaat
     3661 tccacaagat gacctccaga cgtttttata tgtattatca tacactattc cagaaaaaaa
     3721 gaacagttta ttttatctgt atcagtcaaa aatgctggaa aaattaagtt tttcacatac
     3781 aaagcgaact gcactataac aggaaagcgc tgttttatta cactgtttca ggcagttgcc
     3841 aatcaatcgg ttcttctcct aacgcaagga gcgcatcatt cgttttcgag aagggtttgg
     3901 aaccaaaaaa gccacgactt gcagataacg gactgggatg tggagatgtt atgatgatat
     3961 ggcgactctt atcgatcatt ttgatttttt cttgtgccgg ttttccccac aagataaaca
     4021 cgattggttt ttcccgttca tttaattttt caatgatcgt gtctgttaat ctttcccaac
     4081 ctttcccgcg atgagaatac gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa
     4141 ggactccctg tttagcccat gacactagat ttccatgttg aacaggtgcg attccaagat
     4201 catcataaag ctctttatag atatttctta acgatggcgg gatctttacg ccaggttgca
     4261 cagaaaaaga taagccatga gcttgatttt ctccatgata aggatcttgt cctaaaatca
     4321 ccactttaac ttcttcataa ggagtcaatt ccaatgcttc atagatatga tacatatctg
     4381 gatggatctt ttgggacgca tattcttttt ttaaaaattc tcttagatgc agataataat
     4441 cttttgaaaa ttctgaggac aaaacttctt gccaactgtt atgaatgatt tctttcaaaa
     4501 actccacctc ctaataaaaa gaataactca cattttcttc aatcacaagg gacaatcttc
     4561 accctcagca cttgattaat atggtaaact aggaacaaga agcagaaagg aacgataatc
     4621 attatgatca aattaattgc atcagatatg gacggcacat tattagatgc ccatatgagt
     4681 gtttctcctg aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg
     4741 gtcgctactg gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt aggaatcgat
     4801 tgtgcgatga tcactttgaa cggggcacag gtatttgata aagaaggaaa ttcattgttt
     4861 accgtgtcga ttccaaataa tcaagcaatc tctgttttag atattctaga tgctaacggt
     4921 atttactatg aagtagcaac caacgatggt ctttattctg aaaatcaagc gaaacgaatt
     4981 gaaagttttg cttccatggt ggctacccat ttacctcacc ttacgtataa aatggcgatt
     5041 gccatggcct cagctaagct ggagttattg cacatcacat acgtggacag tattcgtgct
     5101 attttggaag aaaagaatct ggaagtttta aaaattattt gttttcatac agaagggcca
     5161 gctgtattag gtccagttgg aaagcagatt tctcttttag gcgatctagc cgttacttcc
     5221 tctggtcaaa acaatatcga agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc
     5281 catgtcgctc acgagcgtgg gatttcttta gaagaagtca tgacgattgg cgataacttc
     5341 aatgatgtca gcatgctgca agtagcagga gtcagtttcg caatgggaaa tgcagaaatc
     5401 gaagtaaaag attatgccaa atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa
     5461 gccatattgc gcgcgattgc agaagacttg tagttttatc tagaaaagag ggatatagat
     5521 gtcagaattt ttcattcagg aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa
     5581 cgaaaaagga cattccctgt ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt
     5641 gtctctttat gccatgaatg gcgatctcgt tgctcacatc aagcagataa gcctgacatt
     5701 cggtacgcgt tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt
     5761 caattggccc ggtgactttt attatattcg tagacttcac tggacagtcc aaggtgatat
     5821 ctataatcat caatatcaaa tccatcattt caatcagcaa atcatgaaaa tggataaagc
     5881 aacacttttt tcaggagatt actatgtatt ggatgtctca aatgatgaag atgctccagt
     5941 ttgtatttgt attgctgcgg ttttagatta ctggctctac cgtaaagaca aaagtcgcga
     6001 taagcctgtt tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata
     6061 cgcatttcag gtacatcttt ttagatattc ttttgcatat gatcataaag atgaccactg
     6121 atcatccgct gaccgacaac tttgaagtca tggcgttcgt ataatttttt agcctttgga
     6181 tttttttcat ccacacttag accgatgacg gagcgattag ctttcttcgc caatttaggt
     6241 aatgcttcta ataatcgcga accgatgcct tgtcctctaa aatcttctga tacactgata
     6301 gagtccaaat accattcgtt gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca
     6361 tctagctgat atttctctaa gataggtacc agtggctgat cgatgattga ttcttcttca
     6421 tctgtataac cgtaagcaac tcctgcaact ttaccgtcta tttcttctac gatacctcgt
     6481 gaataactgt aacgataatt tggatcagtg attgcttctt ctaaaatctc taaagtcttt
     6541 tgttcccctg tctgcttcaa aaatggcagt tccatatctt ttagaatcac taagatcaat
     6601 ggggcgattt ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt
     6661 ctccattcta acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc
     6721 ttttcagtaa tgaaataacc atgagtaacg attcttgccg cttcagttcg gcaataaggg
     6781 cagacgactg gcatttcttt atcattatgt tcgactacat tattgacttc ttcgtatgca
     6841 aatttttttc cgcaattagg gcatttcatt ttttccatgt tatctcaact tcctttcatc
     6901 tttactctct tagcttactc ttatattgta aaaaagagaa gcaatatcct tcttctatgc
     6961 agatatccat acctgacggt cctgttctcc atctattaat cggactttat aataaggacg
     7021 ttcatctttg actagaagtg tccactcttt cacttttgct tttttcccgt agtagctgac
     7081 tgcttccttc atcgctgctt gcggttgttt gaactcttcc gttttttcta tatcaaaaag
     7141 atgttctgcg gccttttccg tgtcatttcg tacggttgtt ttcccagtgg atggattgac
     7201 tgctatgact tgtccttcac aaaaaaactg gtattcatat gttgcattcg taggattctc
     7261 caaatctttt tcaactgcaa aatgaatagc agatatgtta tgttcttttg tttcgttttt
     7321 tacgattgct gctgcttttt tcaatgagat ccgatattcc tggtcactaa tatcatccat
     7381 gtgaaaagcg gcattgagca gtcccgtcat accggtaatc cctattacga tgagggcagc
     7441 aacaccaaac atcatttttt tcattcttca ttctcctgtt tctttttttt aaagaatatc
     7501 aagaaatgaa cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt
     7561 tattcgtgca gttctaatgg caatccgtct ggatcaaaga aaaacgtcat tttttttcct
     7621 gtaaaggtat cttctctaat cggttctgat tcgatcccta aggactgcaa ttctgcaatt
     7681 gtttcttcaa tatcgtctac tttgaaagca agatgacgca agccacaagc ttctggatag
     7741 tttggtcgtt gtggcgcatt agcaattcca aaaatctcca attcagcact tcctagtttc
     7801 agatctaatt tatagtcatc tcgttctttg cggtaatttt ctctgattac ttcaaatcct
     7861 aatttattaa cataaaaatc acgtgatttc tgataatctg aaacaatgat ggctatatga
     7921 tggatcgttg taaggttcat atgagcactc ctattcttcg tttaagctcg gatctatatt
     7981 aatctccagt tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat
     8041 aagaaaaaag gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat
     8101 ataataaatg aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca
     8161 caaattattt ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt
     8221 tagtaaatac agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata
     8281 ttttcagttt ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt
     8341 tttcagtctt ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt
     8401 tattacattt atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa
     8461 atcagtcaac ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc
     8521 agcaaagaac tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc
     8581 cgtattgtta agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattcactgg
     8641 aaaaagctgg tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct
     8701 gattggtcgt tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag
     8761 cttgactgct ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc
     8821 ttcagggctt gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt
     8881 ggcgatgttc gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc
     8941 accttggtat aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa
     9001 cgcacaacaa cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct
     9061 cacgctctaa acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa
     9121 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     9181 cttctgtccc agcctctatt ttttatagta attccttcag atactttctt gcctcatcat
     9241 tgaaacgcag tgattcttct aacttctgat aatattccaa cgcttgtttc tgagcttcag
     9301 taaaacgata atgttttcct ttccatttgt cgattgtctc ttgcacattt ccagattgga
     9361 tatctttcac tactagttgc catttttctt ttgagactgt acggtctttc ttatcagcag
     9421 attgtatagc ttctcgttct ttacgcaata cttttaaaaa accatttctg actatccgag
     9481 gaatgctgtc gaagaaccct tctgtaaaac catagcgaca atattgcttc caaataaata
     9541 gatagatttt tggttggatg aatcgttggt ttttcgcaaa atattccatc tgagcagtga
     9601 cttctggtgt tcgttgacgt tttgtctttc tcaacacgga aataaatgtt tcaaaataag
     9661 cattaggatg tactggcact tcttcatata cgatggattg ccaatcttta cgtaaaaatg
     9721 cgcgatagtt gatcaagaat tcttctacat cctttactaa ttcttctgca aagtcgtcaa
     9781 atagttcttt ttttatctgt cgcttttttg actgcccatt gatgtgttca cttattttct
     9841 tgatttcatt ttccaatgct tgataatccg ctatgtaaat cggcatttct acttggcgaa
     9901 attctgcgag ccgctgagct aattttatat ccggtaaata tggctgtttc atccgacgat
     9961 tttggtacaa gacaaaacag tatttttgcc atagtccttc aggaaaatcg atattcttct
    10021 gcttaagcca taataattcg tctaaggcca gatcaacttt tgtcattccc tgatggatcg
    10081 aagctgccac tgtcggagag acatgcaggt gatcggcaaa gtggttgatg cctagtttta
    10141 ggatctttcc ggtttttggc gatttaacga tgtattgcac ttttaattct ctaccacact
    10201 cacaatacaa tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag
    10261 aaaacaccca atctgttgca gctaaataat gttcttccat aaaacgagag ctgattagat
    10321 atcggcggtg cttatcgact agttcccgct gttttttcgt taattttgca tagatctccc
    10381 aacgttgact gcttgtgtat tgtttagaaa ttttgatggg atagcttttg ctgatttctt
    10441 ttggaaacca ttcttcaaag acggattgcc attcttccac ggctgtttct ttcctttcta
    10501 attacaaaaa gctggctact aacataacgt tagcaaccag ccagtgtttc ttgctaaatt
    10561 agtgagttgt tccggcattt ccggaaatat cttctttatt ttctactgct gcttcaactg
    10621 cataagtcaa agaagaagca atgtcttcta aagacataga aggcgtaccc actggacgat
    10681 cgatcacttg atcgggtgag aatggcacgt ggatgaaccc acctcgaatt gccgggaatt
    10741 gtgtttcgat caaatgaagc aaacgataca tgatatcatt gcagacaaat gttcctgctg
    10801 tataagagat gtacgctggt agaccatgat cttgtacatt ttttgtcatt gctttgattg
    10861 gcaatgaggt aaagtaagcg gctggaccgt tttcttctaa agccgtatcg aatggctgat
    10921 ttccttcatt atcagggatt cttgcttctg ctagattgat tgcgacacgt tcgaacgaga
    10981 ctgctgaccg cccacctgct tgtccgacac agatcacgac atctggttgg tgctgttgga
    11041 tggcttcttt gactacttga ctgctttttt cgaatacggt cggtacttcc actttgatga
    11101 tttcagcacc gctgatttca tctggcattt tttttactgc ttcataggct ggattcactt
    11161 tgtccccacc aaatggatca aaacctgtta ctagtacttt catctgttgt tcctccgtcc
    11221 gattgattta acatatggtt atcttttatc ataccatttt ccttgaacag cgtaaataaa
    11281 ggaaacaata gtttaaataa cataacagat caaacttaat atattcttat aaaaaaatag
    11341 tgttccacta caatgttaga agaggttgcg acaattattc tgtcacaacc cctctcttct
    11401 attatttaaa tagaatcata taagcaattt ggaaaactag cataaataac gcaataaaca
    11461 actggttttt gatcactccg tatttgtctt tcatctccag catcgctaca ggcacgatat
    11521 tgaagttagc agccatcggt gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca
    11581 ttcctattaa cgctggattc gctccatgac taaatacgaa tggtgcaccg atcccaaccg
    11641 tcatcacggt gatggccgca aatgcattcc ccatgattac cgtaaatatc accatgccaa
    11701 gagcgtaaat gatgatgccg atgttcacat ttccttctgg aacgatcgta cctactgcat
    11761 tggaaatcac tgtcccaact ccagcgcttg taaataccgc acctagagaa gctaaaagca
    11821 ttggcaacat gcttaacggc cctactgtgc ccagcatatc tgctgcatca tctaaaaatg
    11881 tacttggtcg attatctcga gaaaagaaca tcaagattag catggctgca aaaacaccga
    11941 cgcccacgcc gactaaagca cctaaattgg taaatagagc aaacaagatc gcaaagacac
    12001 cgatactcaa agctggaata aaaattttca ttcctagttt gtctgacatt ttttccatat
    12061 attcttttga tggaagccgg ctttctcctt tgctgacttt cttagcaatt gctggtatgg
    12121 tcatcgcaaa gatcaagcca ccgttcacga ttgccggaag ccatcttcct cctgcaataa
    12181 ctacccctaa gaccgtccag aaaaaggctg acccataacg atgaggattg gttttatctg
    12241 ttaaatttct tactccagtg taaatcaata cgagtcccat cacgatataa aggatctcca
    12301 gtaatttatc gcctaataaa atatcaggat ttgtaaaaaa gtccatctta ttgccccttt
    12361 cctggataat atttcttcgc taatttcttt tctttaagat agaagtagac acttgccaca
    12421 actaaagccg tgatagccac tggaacttcc actaaagcaa gctgtcccaa tgttacgtca
    12481 tatcctagat ctttcagtgt tgattggact agcaacgccc ctgctcctcc gatgaatagt
    12541 acttggccaa agaaccagca gacattttcc atagctgaag ccataccttt tagttcttct
    12601 tcatgcttag gatggacttc taaccctttt gattcgatgg tccccaatgc catcggtaaa
    12661 atgattggac gaacaaatcc agcaactcca ccaaaactca cgttaaaagc cgcaaagatc
    12721 attcgaaaga tcccataaat atctacaatc ataccagcag atactttctt gaatcgcttg
    12781 atcaaggaag ccgctgattc tttcagacca tttctttcaa gcgttcctgt tgccaacata
    12841 atgatgataa aaatcgccat cccgcgattg tttacaaaac ttgtacctaa aagttctaac
    12901 attccttgaa tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc
    12961 aatatcgagt ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc taaaaggcta
    13021 agataatttt ccatatattt tcctccataa aatgacaata tttttttaat tataaagcaa
    13081 aacacataaa aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg
    13141 ttattttttt attatataat tcaccttact tattgtaaga taaagctttt tttaatttat
    13201 tgcctgctaa tatataaaaa taaggtctag ttactgtttt tttaaatgat taataaataa
    13261 caactccttt ctatgaaatt gcaaagcatt catcttcttt tctgctaacg atatatccgt
    13321 caatgcttgt tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta
    13381 tactacagaa aaagatatac gatctgccac cgggtagtaa aaaagaggat gatcattcca
    13441 ttaggtcttt gaagggatga ttctcctctt tttgtgtatt ttttatattg aaggaggaac
    13501 gtatgtatgg catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg
    13561 atgatgaaat ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattgga
    13621 atgattgcta gtttttactt tttgtcaaag gcaatcaaaa gtttgcctat gagtctctct
    13681 tatcctattt ggacagggat tggcgcagtt ggttcggtca ttattggtgt ggtcttgttt
    13741 catgataagc tgaatctttc cacttggttc tttgtcgggt tgctgctcat cagcatcatt
    13801 ggcatcaaaa tcactagcgg tcactagata cttagacaag cagacaagga ctgatcagaa
    13861 aaagcggggc tttttatttg atattcctct ttctatgaaa aaacaagaaa ataagactaa
    13921 tgacctagcg acttctctac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
    13981 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
    14041 cctcagcgat gtcagtttta gcttatctcc tggcaaaatc gttggattag taggtcctaa
    14101 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagaagg
    14161 aacgatccgt attggtgaaa atgagatttc tccctctagt cataaaggat tagaacaagt
    14221 aggagcattg atcgaatatc cgggtatcta tccattctta acaggttatg atcatctgaa
    14281 gttattctct gaaacaaacg atgtatctgc tatcgacact atcgtgaaac agctcaagat
    14341 ggaaaaatac attcacaaaa aagcgaaatc ctactcgctg ggaatgaaac aaaaattggg
    14401 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
    14461 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
    14521 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
    14581 gatcctgatc aatcaaggaa aaatcatcaa acaatgtaca atggctgaat tgaaccaaac
    14641 agcgcaagat ttcatcgtta tctctacgac aaatgatcaa gctgctcgaa cgatcttgac
    14701 agacgctgga tatcccttgg aagatcctga taaaatcaag cttctaaaaa aaaacgaaga
    14761 gttattagga actgttatcc agttattgtc tgcagaaaat gtccatgtga cagatgtcca
    14821 gcatctgacg aatgatttgg aatcttccct attggaaatt ctggattcag taaaggggga
    14881 agaagaaaaa tgacgttgct tataaaacaa gaattattta aattgatcaa gaaaaagtca
    14941 actgctgttt tatctgttct attagttgct ttgctcatag gaacagcact tctagccaaa
    15001 aaatacacga cgatcattga tccagttgaa atgactgctc agcttttcag tgccacatct
    15061 tggattgtct ttatcatgat tgctgctgca agtacgatca tttcaatgga atcacaatat
    15121 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagtaaa
    15181 tggatcacat tagttattta ttctatttat ttataccttt tagctattat tgtcaccgtg
    15241 ctgatgaaac tgatcctttt cccttcgatt tcctttacgg agaagatttc tactggtcag
    15301 acactgatcc aaagcttatt cacttatact ttgggtagtt atatcggttt atggctaatt
    15361 ttaagtttag tgttgatgat tgcttgtttt atcaatagtt ccggtgcctc catctcagca
    15421 gggatcgtct tttattttgc ttcatcaatc atatctggta tcctcatcgc actcattcaa
    15481 aaatgggagt ggatcaaatg gaatccaatc agtatgctga atctgcaaaa ccaaattggc
    15541 aacgaagaga ttatgaagca attgactcat ctttctacta atcaaatgct gtttggcaat
    15601 ttagcttaca ttgctttgtt cttagcattg ggatacctcg tattcaagcg aaaaaatgta
    15661 tgatggttca ttaacaaaga agttgtgaca gaagtggcca gtttcaagaa atttcggctt
    15721 atttttagaa gaagctactt cttgaacacc atttattcaa aaaaagaagc gatgacacga
    15781 atgttgtcac cgcttctttt t
//
LOCUS       DACIWG010000057        15739 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.060, whole genome shotgun sequence.
ACCESSION   DACIWG010000057 DACIWG010000000
VERSION     DACIWG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.060"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(30..314)
                     /locus_tag="IYF54_002104"
     CDS             complement(30..314)
                     /locus_tag="IYF54_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ9832422.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(304..1038)
                     /locus_tag="IYF54_002105"
     CDS             complement(304..1038)
                     /locus_tag="IYF54_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ9832423.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1167..1790
                     /locus_tag="IYF54_002106"
     CDS             1167..1790
                     /locus_tag="IYF54_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ9832424.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1982..3103
                     /locus_tag="IYF54_002107"
     CDS             1982..3103
                     /locus_tag="IYF54_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ9832425.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3110..6238
                     /locus_tag="IYF54_002108"
     CDS             3110..6238
                     /locus_tag="IYF54_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ9832426.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6257..9451
                     /locus_tag="IYF54_002109"
     CDS             6257..9451
                     /locus_tag="IYF54_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ9832427.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9597..10304)
                     /locus_tag="IYF54_002110"
     CDS             complement(9597..10304)
                     /locus_tag="IYF54_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9832428.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            11069..11467
                     /gene="spxA"
                     /locus_tag="IYF54_002111"
     CDS             11069..11467
                     /gene="spxA"
                     /locus_tag="IYF54_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ9832429.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            11799..12452
                     /locus_tag="IYF54_002112"
     CDS             11799..12452
                     /locus_tag="IYF54_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ9832430.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            12678..13676
                     /locus_tag="IYF54_002113"
     CDS             12678..13676
                     /locus_tag="IYF54_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ9832431.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            13852..15660
                     /gene="pepF"
                     /locus_tag="IYF54_002114"
     CDS             13852..15660
                     /gene="pepF"
                     /locus_tag="IYF54_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ9832432.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         5475 a         2635 c         3243 g         4386 t
ORIGIN      
        1 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
       61 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
      121 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
      181 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
      241 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
      301 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
      361 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
      421 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
      481 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
      541 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
      601 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
      661 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
      721 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
      781 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
      841 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
      901 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
      961 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
     1021 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
     1081 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
     1141 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
     1201 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
     1261 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
     1321 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
     1381 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
     1441 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
     1501 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
     1561 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
     1621 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
     1681 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
     1741 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
     1801 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
     1861 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
     1921 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
     1981 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
     2041 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
     2101 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
     2161 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
     2221 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
     2281 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
     2341 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
     2401 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
     2461 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
     2521 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
     2581 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
     2641 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
     2701 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
     2761 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
     2821 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
     2881 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
     2941 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
     3001 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
     3061 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
     3121 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
     3181 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
     3241 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
     3301 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
     3361 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
     3421 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
     3481 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
     3541 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
     3601 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
     3661 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
     3721 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
     3781 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
     3841 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
     3901 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
     3961 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
     4021 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
     4081 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
     4141 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
     4201 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
     4261 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
     4321 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
     4381 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
     4441 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
     4501 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
     4561 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
     4621 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
     4681 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
     4741 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
     4801 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
     4861 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
     4921 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
     4981 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
     5041 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
     5101 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
     5161 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
     5221 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
     5281 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
     5341 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
     5401 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
     5461 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
     5521 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
     5581 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
     5641 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
     5701 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
     5761 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
     5821 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
     5881 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
     5941 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
     6001 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
     6061 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
     6121 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
     6181 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
     6241 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
     6301 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
     6361 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
     6421 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
     6481 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
     6541 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
     6601 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
     6661 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
     6721 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
     6781 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
     6841 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
     6901 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
     6961 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
     7021 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
     7081 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
     7141 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
     7201 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
     7261 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
     7321 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
     7381 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
     7441 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
     7501 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
     7561 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
     7621 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
     7681 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
     7741 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
     7801 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
     7861 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
     7921 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
     7981 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
     8041 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
     8101 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
     8161 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
     8221 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
     8281 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
     8341 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
     8401 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
     8461 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
     8521 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
     8581 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
     8641 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
     8701 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
     8761 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
     8821 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
     8881 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
     8941 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
     9001 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
     9061 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
     9121 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
     9181 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
     9241 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
     9301 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
     9361 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
     9421 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
     9481 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
     9541 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
     9601 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
     9661 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
     9721 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
     9781 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
     9841 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
     9901 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
     9961 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    10021 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    10081 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    10141 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    10201 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    10261 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    10321 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    10381 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    10441 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    10501 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    10561 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    10621 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    10681 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    10741 actattaaag aaaaaaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
    10801 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
    10861 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
    10921 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
    10981 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
    11041 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
    11101 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
    11161 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
    11221 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
    11281 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
    11341 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
    11401 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
    11461 attatagaag gataatacga aacttggaaa agactattac aaagatagtt tttccaagtt
    11521 tttttgtttt tacaaaaagt attcttgatt agatgtataa aaaggtagta gaagatgatg
    11581 attgcacgaa aaatagagag ggaaaattag ctgaaaagtc ggctatatag tattcgtctg
    11641 ccaaaaatgt tatagttaaa taagatagct tgaaaggttg aaaaatccgc tggttttatt
    11701 aaattcgctt aacagcgaga ttttttttct ttacatttaa gctgatttcg ctacaatgaa
    11761 tctatattaa acattgtggc aaagtgaggt gtagcaaaat ggaaatggaa catattaatg
    11821 aaaatacgat ccgtgtacta attggtcatg aagatttaga ggaacggggc gtttcatttt
    11881 tggatttact tggcaatcac aaagaaatcg aaaatttctt ttatagtatt ttggaagaag
    11941 ttgatatcga agaggagttc cgaggaagcg aagctgtcac tttccaagtt ttgccaaaag
    12001 gagacggatt agaactgttt atcagtaaaa atcttccgga tgaagaaatt tctcaatcag
    12061 aagattctac tgttgattca gaagatgtga gtgactttct tcgtcagcag attattggca
    12121 gcgatccgga ggaagaatta tctggaagcg ccctagaaga acaggaaaga caagcaactt
    12181 ttgcttttga agattttgaa caagtgatcc aacttgcaac agatgtcgaa ctagagtctg
    12241 cttggactga tttgtattta ttcgaagagc gctattacct agtcgttcac ttctggatgg
    12301 aaaatctcaa ccaagcggat gtagagaatc agattgctcg aattttggag tttgctgaaa
    12361 agagcgatcg tacaccagaa acattgaatg aatatggaca atgtctgatg gcgcgtaatg
    12421 ctatcgagcg aacgaaattt tattttgtat aaatcagcat tatagtaaga gactgtgaca
    12481 aaaattgtgt cgcagtcttt ttttcgtata aatgtgttca aaagcttgca tctgcggtaa
    12541 caagcccaac caaacgaaaa atattgggaa ctatttttgt ttagctgttc ttattacttg
    12601 atgcagaata gctttttcac gccctctttt ttcgtgtaaa taaattctta gacgtaaata
    12661 agttcagagg gagaaaaatg ctgcttgcaa tgaatgaata taataagtgc tgttttgcta
    12721 aacatgcaga aaaaggaaag aaatattatt gtcgtggctg tcgaaaaaga gtgattttga
    12781 aaaagggaaa gaaaaaatgt gctcattttg cccatcaaaa atcagataac tgttcggtct
    12841 ttagtgaaag agaatcaaaa gagcacttac agttaaagga atgcttcatg gattggctgg
    12901 gacagtcagt agaaccagta tttttggaag cctatttgcc gaggttgcgg caaaggccgg
    12961 atattttgtt ggcgaatcta gcaatagaaa tccaatgttc tagactgtct caccagagat
    13021 ttattgaaag aacccaaaat tatttgaata acagttacca agtatggtgg atacttggta
    13081 attcttttct tggacaatcg caattctcat tgatagaaaa aagttgctgc tactataata
    13141 gaaaaagagg agtgcattgc tggaaagctg atctcaagaa acaaaagctt tatttgtatc
    13201 atcatattac agagactgta tcagggcata tttccttttg cagtagctgc tggacttttt
    13261 ctagtaggga ccttaaagaa atattcacta gtaacgaaat taaaatgaac caaatgaaaa
    13321 aaatagaaaa agtgagtgaa gatgggaaga aatggctgac acgccaactt attcacaagc
    13381 aaaagaatac agtatccata caagaacaat gctacttgcg tcataaacat cttttgcatc
    13441 tatcatcgtg gatctatcaa aaaagtcggt ttttctttta tttacaagaa caggtttttc
    13501 tttatcgaat gttgtacgaa gaagccttgg aaaaccagaa agttcctgat ttcaactcat
    13561 ggctttgcca aataaaagaa cataaaaggg aatggctttt tcctatgatt gatgaagaga
    13621 tggtctatca gcagtttttt aacgaatgca tacacttgtc ttctttgaaa aaatagtaag
    13681 tataaaaata ggctaggaat cggttttcta tgtaaaacaa gatttagtta tgctaaaatt
    13741 gatggaagca gacacatgaa tgagtagaaa agttagatgc tgtgacatgt ggatgttaga
    13801 ctatgatcta ttttgataga tagagctaag ggaagaaaag gagtggattt catggaagta
    13861 aagcagttgc caaaacgaga agaattgcct gaaaatttaa cttgggactt gaccaagatc
    13921 ttttcaagcg accaagagtt tgatgagaaa tatttggaat tatcagaaga gttaaaacaa
    13981 tctgaaaaac acaaaggaac acttgatcaa ggcgcttctc aatttttaaa tgcgattgaa
    14041 ttcgtattga gggtttatcg ccaaactgaa gtcatttatg tatatgcgca ccttaaaaac
    14101 gatcaagaca ctggaaatac agattaccaa gcgctttatg caagagcaag cagtctgttt
    14161 tcgaaagtta gtgaagccgt ttcctggttt gaaccagaaa tattgcaatt gtcagatgac
    14221 cagatttggc aatatttcaa agaagaacca aaattggaag tctatcgcca ttatatccag
    14281 caaatagtag ataatcgagc ccatgtctta tctgctgagc aggaatctct tcttgctgga
    14341 gcaggtgaaa tctttgatgc ttcaagtgat acatttgctg ttttgaataa tgcagatcta
    14401 gtttttccaa cgattgaagg agaaaatggt gaaatagtcc aattatctca tggcgtgtat
    14461 ggtcagttgc tagaaagcac ggatcgaagg gtgcgcgaag cagcatttaa ggggttgtac
    14521 agtgtttacg aacaatttag aaatacattt gcttctactt taggcacaca tataaaagga
    14581 cataatttta aagcgaaagt ccgtaattac agctctgcca gagaagcgtc tttgagcaat
    14641 aatcatattc ctgaaagtgt atacgatact ttggtagacg tggtaaacaa gcatttgcct
    14701 ttgttacatc gatacatgga attacggaaa cgtttattag aagtggaaaa actgcacatg
    14761 tatgatcttt atacaccggt cttaggggaa gctccaatta cctttacgta cgaagaagca
    14821 aaagaaaaag ctttagaagc actgaaacca atgggtgaag aatacatggc catcgtagaa
    14881 aaagcattct ctgaacgttg gatcgatgtt gtcgaaaata aagggaaacg aagcggtgct
    14941 tattcttcgg gaagctatga cacaaatcca tatattttat tgaattggca tgatacgctg
    15001 gatcagctat ttacgcttgt ccacgaaatg ggacatagtg ttcatagtta tttcactcgt
    15061 tcgaaccagc cttatgtgta cggcgactac tccatctttt tagcagaaat tgcttcgaca
    15121 acgaatgaaa atatcctaac ggagtattta ttggaaacag aaaaagaccc tcgtgtacgg
    15181 gcttatgtac tcaaccatta cttggatggg tttaaaggaa cagttttccg ccagacacaa
    15241 ttcgctgaat tcgagcattt catgcatacg gaagatgaaa aaggtgtgcc attgactagt
    15301 gaatacctaa gtgatagtta tggtaaattg aatgcaaaat attatggtcc agcagtcgaa
    15361 gaagacccag aaattaaatt cgaatggtca cggataccgc atttttatta taattactat
    15421 gttttccaat actcgactgg cttttctgct gcttcagcac tggcgaagaa aatactaaac
    15481 caggaaccag aggcactaga gaactacttg gcttacctaa aagcaggtaa cagcgattat
    15541 cctgtggaag taatgaaaaa agcaggagtt gatatgacac aggctgcata tattgaagat
    15601 gcaatgtcga tgtttgaaca acgtctaaat gaattagaag aattgatcga tcgtttataa
    15661 ggctttctat ttttgatttt ctcgaataga aacgaaaaaa atacgtcggc tctcttgtaa
    15721 gggagtcgac gtatttttt
//
LOCUS       DACIWG010000058        15401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.061, whole genome shotgun sequence.
ACCESSION   DACIWG010000058 DACIWG010000000
VERSION     DACIWG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.061"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(131..1426)
                     /gene="purB"
                     /locus_tag="IYF54_002115"
     CDS             complement(131..1426)
                     /gene="purB"
                     /locus_tag="IYF54_002115"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ9832433.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1439..2572)
                     /gene="purK"
                     /locus_tag="IYF54_002116"
     CDS             complement(1439..2572)
                     /gene="purK"
                     /locus_tag="IYF54_002116"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ9832434.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2556..3044)
                     /gene="purE"
                     /locus_tag="IYF54_002117"
     CDS             complement(2556..3044)
                     /gene="purE"
                     /locus_tag="IYF54_002117"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ9832435.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3460..4767)
                     /locus_tag="IYF54_002118"
     CDS             complement(3460..4767)
                     /locus_tag="IYF54_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ9832436.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4770..5351)
                     /locus_tag="IYF54_002119"
     CDS             complement(4770..5351)
                     /locus_tag="IYF54_002119"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ9832437.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5467..5564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5923..7215)
                     /locus_tag="IYF54_002120"
     CDS             complement(5923..7215)
                     /locus_tag="IYF54_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9832438.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7212..7835)
                     /locus_tag="IYF54_002121"
     CDS             complement(7212..7835)
                     /locus_tag="IYF54_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832439.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7838..8674)
                     /locus_tag="IYF54_002122"
     CDS             complement(7838..8674)
                     /locus_tag="IYF54_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ9832440.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8691..9578)
                     /locus_tag="IYF54_002123"
     CDS             complement(8691..9578)
                     /locus_tag="IYF54_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ9832441.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9594..10877)
                     /locus_tag="IYF54_002124"
     CDS             complement(9594..10877)
                     /locus_tag="IYF54_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9832442.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11560..12243)
                     /locus_tag="IYF54_002125"
     CDS             complement(11560..12243)
                     /locus_tag="IYF54_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ9832443.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12261..12878)
                     /locus_tag="IYF54_002126"
     CDS             complement(12261..12878)
                     /locus_tag="IYF54_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ9832444.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12888..13712)
                     /locus_tag="IYF54_002127"
     CDS             complement(12888..13712)
                     /locus_tag="IYF54_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ9832445.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13705..14265)
                     /locus_tag="IYF54_002128"
     CDS             complement(13705..14265)
                     /locus_tag="IYF54_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832446.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14439..15395
                     /locus_tag="IYF54_002129"
     CDS             14439..15395
                     /locus_tag="IYF54_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ9832447.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4530 a         3429 c         2633 g         4809 t
ORIGIN      
        1 ttaaaatcag gttcctatta ttgataagaa aatataataa actaaaagaa gcagaaccgc
       61 taacggatct gcttcttttt tggttatttt tgctattcta ttttaattga attttgcaac
      121 tcactcttct ttattccaaa cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat
      181 gatgataatc aaaggcatcg tctatttctt ctttagataa taccgatgtg atggttggat
      241 cttcttctaa cagcgggcgg aactgcactt ggttgtccca agcgtacgca gtttttggct
      301 gaacaagatc ataagcttct tctcttgtca ttcccttatc gatcaatttc agcataacgc
      361 gctggctata aatcaaacca aatgttgcag acatattccg cttcatgttt tcaggaaaga
      421 ccgttagatt tttaacgata ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg
      481 tatcaggcaa gatgattcgt tctgctgaag aatgagaaat atctctttca tgccataatg
      541 tgacattttc ataggcagtg accatgtgtc cgcgcaccac tctggctaga ccagccatat
      601 tttctgaacc gatcggattc cttttgtgcg gcatagcaga agaacctttt tgacctttcg
      661 caaaaaattc ttctacttca cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa
      721 aacgttcaat acttgtagca ataagcgcca tcgctgagaa atattcagca tgtagatccc
      781 gagggagaac ttgagtagaa atttcttgtg gacggatccc caattgttta catacatact
      841 cttcaataaa tggcgggata tttgcgaacg ttcccactgc tcctgagatt tttcctgctt
      901 ctactccttt tgccgcatgt tcaaatcgtt cgatgttccg tttcatttct gaataccatg
      961 tggcaagttt caatccaaaa gtagttggtt cagcatggac accgtgtgtc cgacccatca
     1021 tcactgtata tttgtgttct tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt
     1081 cttcccgtaa aatatcattt gcttgcttga tgagatagcc ataagcagta tctacgacat
     1141 ccgtacttgt tagaccataa tggacccatt ttctttcttc tcccaatgtt tcagaaaccg
     1201 ctcgtgtaaa cgcaacgaca tcatggcgtg ttaatttttc gatttccaag atccgatcga
     1261 tatcaaatga tgcatgctca cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt
     1321 ctgcccaagc ttcatctgct aaaatctcga cttcgagcca cgcacggtat ttgttctctt
     1381 ccgtccagat attccccatt tcagggcgtg tataacgttc taacatgttt tttctcctct
     1441 aatcccaaat attcgtttgt ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga
     1501 tagtgatatg tcccatcttg cgaccttttt ttgcttgttt ttttccatag aaatggaatt
     1561 gccagtctgg cttattctca atcaagcgat agctttccag cagttgttct cccaaaacat
     1621 tgaccattac tgcattgctt atcaaacgta ctttttctcc taatggccaa ccacaaatac
     1681 ctcgaatatg ggcatcgaac tgactcatag aacaagcttc aatagaataa tgtccagaat
     1741 tgtgaggacg aggagctaat tcattcacat atagcccgcc tgtttttgtt aaaaacattt
     1801 ctacccctaa aacacctgaa aggctcactg cttctgcaat cacacgggca atacgctcag
     1861 cctcctcgat cacatcctga tcaatcgcgg ctggagcaat cgtttcatga agaatattgt
     1921 tgtggtggat gttttccacg accggaaaag tcgtgaaatc tccttgacca tttcctgcca
     1981 ccatgatcga aatctctttt tcaaacggga tccatgcttc taaaacacag gttccttctc
     2041 ttaaaaggtc catggcagga gctagatccg cccgactttt caaaacatac tgccccttgc
     2101 catcatagcc gcctcgagtg gttttcaaaa cacaaggata gccgatcccg tcaatcgcat
     2161 cttggatatc cgtcggactg acgatcgttg cataaggagc aatcacgatg ttattcgttt
     2221 ccaaaaaaga tttttctaac aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag
     2281 gaataaatga catgggtaaa atcgcattta acgcctctac actgacgttt tcgaattcat
     2341 aggtaatgac atctgttctt ctggcaagct ctttcagcgc aaaggtgtca tcatagacac
     2401 tttcaatatg ccaatctgca acttgtgcgg ctggacaatc gctcacagga tccagcacac
     2461 ctactttaaa gcccatttct tttgcactaa tcgccatcat acgacctaat tgaccgccac
     2521 cgacgatccc gatcgttgaa ccaggcaaca aaggtttaac caagttgatc actactttct
     2581 ataacagttt tagccaacat tgaacgtctt tcagccaatt tagcctccaa ttctaaatca
     2641 tacatggaaa gcatctgtat cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt
     2701 gctgtagtag ctacaggtac tcctccaggc atttggacaa tcgaaagaag cgaatccaaa
     2761 ccatttaatg ttcgtgattg gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg
     2821 accatacccg gcagatgcgc ggctccccct gctcctgcaa taatgatttt gattcctttt
     2881 tttcgagcgt tttgtgcata ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc
     2941 tttttttcat aagacacacc gaattcatcc aatatttgac atgcttcttt cattgtttcc
     3001 caatctgaag tactccccat aatcacagag atttttgctg tcattgtttt tcctcccagt
     3061 ttttctacat tcatatttta acaatcactt tttttgatgt caaagaaatt tcgaatgtta
     3121 acaaagaaaa acattttatt gttcgtgttt attaaagata aaaaaaagga atgtgacaaa
     3181 agttaggtca cattcctttt tatggataaa cggtgttctc ctgccaactc ctcggtcaca
     3241 aggaacaata ttcaacaaac atgagaagct tttttttgaa attgttcctt gtgactcgga
     3301 gatgaacggc aggctcacga cctcttcctt caacggtgtt caatcagctg accctcggta
     3361 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctaacaatc
     3421 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
     3481 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
     3541 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
     3601 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
     3661 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
     3721 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
     3781 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
     3841 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
     3901 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
     3961 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
     4021 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
     4081 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
     4141 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
     4201 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
     4261 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     4321 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     4381 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     4441 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     4501 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     4561 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     4621 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     4681 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     4741 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     4801 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     4861 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     4921 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     4981 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     5041 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     5101 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     5161 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     5221 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     5281 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     5341 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     5401 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     5461 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     5521 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     5581 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     5641 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     5701 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     5761 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     5821 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     5881 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     5941 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     6001 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     6061 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     6121 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     6181 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     6241 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     6301 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     6361 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     6421 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     6481 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     6541 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     6601 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     6661 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     6721 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     6781 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     6841 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     6901 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     6961 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     7021 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     7081 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     7141 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     7201 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     7261 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat gctgtataca ccagaacagt
     7321 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     7381 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     7441 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     7501 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     7561 tgaaacaacg taatgatgaa acacatccgt ttgatttttc ctttcctcca acataccttt
     7621 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     7681 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     7741 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     7801 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     7861 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     7921 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     7981 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     8041 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     8101 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     8161 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     8221 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     8281 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     8341 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     8401 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     8461 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     8521 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     8581 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     8641 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     8701 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     8761 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     8821 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     8881 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     8941 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     9001 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     9061 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     9121 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     9181 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     9241 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     9301 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     9361 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     9421 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     9481 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     9541 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttcctcctg ctgctatcct
     9601 agaatgtttg acgcaaactc ataaagactc tctgctgctt cttctgctgt tacttcttca
     9661 taaatgactt tttcaactaa aagattgtat tggtcttgaa tttccggatt caaagcacta
     9721 tcaaagacat tcacttcgcc gttgtccata tcggaaatca tttctaaata atcaaatgtc
     9781 gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc gaatattcga catgattggt
     9841 acaccacgtt ctccttttaa aatttcattg gcttcttcac tgttttgaaa gaaatcaata
     9901 aacttagcag cttcttcttt atgggctgag ctttttgcca tactcagcat ttgcgaagaa
     9961 cgtaaaggga taccagattc tccatctgct tttctgcgag gtggattaat caactgaatc
    10021 tccatatctt caggagcccc tgacaatatt tctgtcaact ggttacttga tagaaacacc
    10081 attgccgctt ctctatccct aaccgcctga aaagacagat ccgtcgtact tgcagctcca
    10141 ccacctggtt ctggatgggc ccctttgtct accagatttt tacgcagctg gaaaaaatct
    10201 gttagatagt ctggattttc aaatcccaag ctgttagata gatcttcccg attataaaaa
    10261 ttcaatcctt tctcaacttg tggtatttga ataatacagc cagctataaa atcttcgaat
    10321 cttgaaatag catagattcc taattcttca gtgatttgat agctgatttg ttcaaattca
    10381 tcccatgtcc aatttgaagc tggttcaggg atatttaatt gttcaaatat atttttattg
    10441 taagcaatcg caaacgaatt caccccgtta gacagtccaa ctaattgccc gtcgtaagtc
    10501 gtcgttgcta aataggattc atctgcatta gaaatatcaa ttatcccttg ctccacatat
    10561 tcatctaatg gttcaatctg attgatgtat tgaggaaatt gattgttcag ttggaaaatg
    10621 tcccagatat catctgatgc tgctagtgta cctaattttg tgtaatatcc ttcataatca
    10681 taaaattcat aatcgatcgt cacatgggga tttttttctt cataaagctc gattactttt
    10741 actgttgcat cgtgtcggac ttgcgaaccc caccaagcca tgttcagcgt gatttttttg
    10801 tcatcttctt gcccacaagc cgtcaagaag aataacataa cacttaaagc taagatgatt
    10861 ctcattcttt tcttcattct ctgcacctct acttccattg ttatttttca atatactgta
    10921 tttttataca gaacatattt attaaacgat ctttttcaag gggaataaaa accaagactt
    10981 gccttatcgt tctgcctagg atgaacaact caatactttc aagaaaaatt agatagtgct
    11041 gattcttctt tcatcgctaa aaacacttct tgcttcaatg atgggaggat aattacgcaa
    11101 agaaaaaagg atatcagaca actgcccaat atccttgaca tagctaaata agcatgcttc
    11161 ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag taattctgta tgaaattagg
    11221 tacaatatac acgtcgatgc acaagcattg actatggcaa cttgattggt cagttcccgc
    11281 tgacttttca agccccgaca atttagctgc tactaaattg tcgggtgcct tttttagttt
    11341 ttattcgttt ttcattctat agaaagcgct tcaataaatc aagacatttt tttgttagaa
    11401 tgctaataat aacaaaaggt tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa
    11461 aaatttctct tttttttgaa ttctgaacaa atagtactgg agtacaaaag acgaggaaga
    11521 gccactatct gctcttcctc gtcttttgat tctcacctgt tattcatgat tctttttctc
    11581 agtttcccga tatttttcca ttttacgact aaaaagctct ccgctacttg gtggcgtata
    11641 tgtgatggca tttgctcctg cttgaatggt ctcagcaata ctttcttccg ttggaccacc
    11701 tgtcgcaatg ataggaagtt caggatattt tttacgaaaa tggcgtactg cttctgcagt
    11761 ttctttacct gcactgatat tgataatcga aataccagct gctaactttt catctatttc
    11821 tgtatatttc gacgttaccg tactgataac tggaatatca actactcgac aaacttcttt
    11881 tactgtctct aaaggcgtag gaccgttcaa aacgacccct aacgaacctt gcgcttctgc
    11941 gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa
    12001 aactggaatg tcagctgcct caataatgct tttagtaatc gctggatgag gtgtaaaagg
    12061 atacacagca attactgcat tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa
    12121 gatgatcgaa cggatcaatt ttccaaatat cttgatcccg cttgcttcta aaatgatttc
    12181 cggtactctt acgatatctt ttctcaattc cgtcatgatt tctggcgtaa attgttctgc
    12241 catcagccac ttctcctttg ctatctccgg tcatactgat tcaattgata cgtctcgatg
    12301 acctggatga ttttttgcgc ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct
    12361 tgtgcggctt cctgatagtt ggtggccgta atcacttttt catacagcgc aggattccaa
    12421 tctactcctt ggacgaacag ttgtgtatgg tcatccatgg actgttccca tgaatcatac
    12481 actttgaagt ctccttggat agttatccat tcttcgttaa caaattcctt tgtttccagg
    12541 cttactttct tctgatcccc ataagccttg atcccgaaaa gattattgta tttgctagcc
    12601 aaggtacttt ttccccaatt tgattctaat atagcttgtc ctaaaatgat actaggaagc
    12661 actccataac catcttgtag ttcttgggca tgcggaacaa gccgttcaat aaattgctga
    12721 tgggattgcg cttcattttc ttgctgatta tcagtaaaag gagaagacag attcttcaag
    12781 gaaaaaacaa aggctaagag tatcactgct acaccggcaa ccactgctgg ccaacggata
    12841 ttcttgcgct tttgcttttt ccactttttt ttcgccatga tcaattccta cttttcattt
    12901 aattcattca gatattcttc tagcgtcaat tggtgtttgg tgatatactc agcgttttct
    12961 ttaccgacat aacgtaagtg ccaaggttca tatgtgatct tagttatatc ttcgtgtcct
    13021 tttggataac gaacgataaa tccatactgc gcagcattat ctgcgatcca tttagctcca
    13081 ggctgatctc cgtaacttgc atccaataca gtagaaggat aactgttgta ccagttttga
    13141 tcgacgacat cgactgccag acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt
    13201 tctttagtaa tctttgtcgc ctcctcttct gatacacctt gacgactcat cacatcttgg
    13261 acattttgtt cgaaaacttg ttgttgggat gatacagacc ggtaagctga gaccatcact
    13321 aatggaaatc cagctgcttc agcagccttg gcaaatgctt catattctgg agcaatgcgt
    13381 ttgtcgatca agtatccgtt acttaatgat gccaattgag tagactcatc aatttctttt
    13441 tctaatttgt gatctggacc aaccaaaact agcttccaat cgtctgtttt aacatctggc
    13501 aattgtttgg acgtacgatc ttttgtttct ttgtccttta ccttgctgct ggttgtttga
    13561 ctgttctttt ctttactagc agttgttttg gtgcttgttc gttcgactgc ttgattgccg
    13621 gcttgaacat gcgaatcatc atcgctaaaa ataacatagc ccattgctgc gatcataatc
    13681 acaactgctc caaatcctaa aaatttattc acttgaaaaa tcctttcgtt gttcttcacg
    13741 ttccccattc gcctcatgaa cgtaagtttc ttccagattt tcattcaccc aatcgagaag
    13801 ttcgattttt tctccttcga tctgtttttg aacatctgct ggcagacgaa aattcaaaat
    13861 atcatcatag ccccattcat catattgaat acttactttc aagtcaaggt atctaccagc
    13921 ctgctgatta tttttcaaag ggatcagctc gacttttgct gctttgctgc taagccattt
    13981 ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg
    14041 aaaaataatc gtttgacgca aaatcttttg cgttagcatc cattcataat cagtgaaaag
    14101 attttttact ttctgcatcg cttcagatgg cagatttttc tcgccatctt tccatttttg
    14161 ccattgttct ggactgatca gcaagtattc atctaaaaaa tttttttcag tttcaaactg
    14221 ttctaataat acactcataa taagatcaat atatacttga tgcacgttta tcccctcctt
    14281 ttaactattc tacttaagtt ttgatcaaaa agcacctctt aaatcgctgc tttcattttt
    14341 tttaatgttt caagtcctct cctctttatt cttcgtgatt tttgttataa taatagacag
    14401 acaagaaact caatccaact atgaaggagc gttttcagat gaaaaaatat caatggggaa
    14461 tcgttggtct tggtaccatc gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg
    14521 aactaacagc agttgcttcc agaacttcag aaaaagcgga aaacttcgct caccgttaca
    14581 atattccaaa agcttacgga agctatcaag aaatgttaga cgatgctgaa atcgacatcg
    14641 tgtacattgc tgtaccaaat agacagcata tcgaccatat cttggcagca ttggaagcag
    14701 gcaaacacgt tctatgtgaa aaagccatca cgatgaataa aaaagaactg gctgacgcta
    14761 tgcgcttagc agaagaaaag aatttgatct tagcagaagc gatgacaatc ttcaacatgc
    14821 cactttatca gcagttacgc tcaataatgg atacaggaaa actgggggct ttaaaaatga
    14881 tccaagcacc tttcggcagt tacaaagaac cagaccctaa aaaccggttc ttcaatccag
    14941 aattagctgg aggagctctg ttggatatcg gtacgtatgc tgtatctttc gctcgtttct
    15001 ttttgagctc tcaacctgaa gtagttgcct ctacaatggt tccttttgaa acaggcgtgg
    15061 acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga acttgctgct gtcagcttga
    15121 cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt tgagaacggt tatgtgacga
    15181 ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt tacagatggt acaaaggaat
    15241 tcatcgaaag cgggaataca tctgaagcaa tgaattacga aataaagaat atggtacaca
    15301 tgattgaagg tgacttgccc aatcgctctc tcttcttgac aaaagatgtc atcgaaatct
    15361 tggatcaaat gcaagttcta tggaaaaata tgtaatttta a
//
LOCUS       DACIWG010000059        15244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.062, whole genome shotgun sequence.
ACCESSION   DACIWG010000059 DACIWG010000000
VERSION     DACIWG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.062"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            168..362
                     /locus_tag="IYF54_002130"
     CDS             168..362
                     /locus_tag="IYF54_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832448.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IYF54_002131"
     CDS             631..930
                     /locus_tag="IYF54_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832449.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IYF54_002132"
     CDS             complement(1027..1461)
                     /locus_tag="IYF54_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832450.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IYF54_002133"
     CDS             complement(1693..2403)
                     /locus_tag="IYF54_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ9832451.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IYF54_002134"
     CDS             complement(2393..3253)
                     /locus_tag="IYF54_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ9832452.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IYF54_002135"
     CDS             complement(3258..3896)
                     /locus_tag="IYF54_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ9832453.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IYF54_002136"
     CDS             complement(3911..4210)
                     /locus_tag="IYF54_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9832454.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IYF54_002137"
     CDS             complement(4253..5737)
                     /locus_tag="IYF54_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ9832455.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IYF54_002138"
     CDS             complement(5757..6218)
                     /locus_tag="IYF54_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9832456.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IYF54_002139"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IYF54_002139"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ9832457.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IYF54_002140"
     CDS             7521..8291
                     /locus_tag="IYF54_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9832458.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IYF54_002141"
     CDS             complement(8564..9391)
                     /locus_tag="IYF54_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832459.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IYF54_002142"
     CDS             9959..11194
                     /locus_tag="IYF54_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ9832460.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IYF54_002143"
     CDS             complement(12193..12453)
                     /locus_tag="IYF54_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ9832461.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IYF54_002144"
     CDS             complement(12480..13781)
                     /locus_tag="IYF54_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9832462.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IYF54_002145"
     CDS             complement(14073..14531)
                     /locus_tag="IYF54_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ9832463.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..>15244)
                     /locus_tag="IYF54_002146"
     CDS             complement(14544..>15244)
                     /locus_tag="IYF54_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ9832464.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
BASE COUNT         4876 a         3105 c         2365 g         4898 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcaaatgcgt
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacgac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttat
//
LOCUS       DACIWG010000060        14093 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.064, whole genome shotgun sequence.
ACCESSION   DACIWG010000060 DACIWG010000000
VERSION     DACIWG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.064"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(449..2446)
                     /gene="tkt"
                     /locus_tag="IYF54_002147"
     CDS             complement(449..2446)
                     /gene="tkt"
                     /locus_tag="IYF54_002147"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ9832465.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2548..2799)
                     /locus_tag="IYF54_002148"
     CDS             complement(2548..2799)
                     /locus_tag="IYF54_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ9832466.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2946..3569
                     /gene="lexA"
                     /locus_tag="IYF54_002149"
     CDS             2946..3569
                     /gene="lexA"
                     /locus_tag="IYF54_002149"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ9832467.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SNFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3748..3966
                     /locus_tag="IYF54_002150"
     CDS             3748..3966
                     /locus_tag="IYF54_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832468.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4254..4766)
                     /locus_tag="IYF54_002151"
     CDS             complement(4254..4766)
                     /locus_tag="IYF54_002151"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ9832469.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="IYF54_002152"
     CDS             complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="IYF54_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ9832470.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7219..7653)
                     /locus_tag="IYF54_002153"
     CDS             complement(7219..7653)
                     /locus_tag="IYF54_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ9832471.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7663..8451)
                     /locus_tag="IYF54_002154"
     CDS             complement(7663..8451)
                     /locus_tag="IYF54_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9832472.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8448..9389)
                     /locus_tag="IYF54_002155"
     CDS             complement(8448..9389)
                     /locus_tag="IYF54_002155"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ9832473.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9566..10153)
                     /locus_tag="IYF54_002156"
     CDS             complement(9566..10153)
                     /locus_tag="IYF54_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ9832474.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="IYF54_002157"
     CDS             complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="IYF54_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ9832475.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11975..12985
                     /gene="gap"
                     /locus_tag="IYF54_002158"
     CDS             11975..12985
                     /gene="gap"
                     /locus_tag="IYF54_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ9832476.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(13070..14005)
                     /locus_tag="IYF54_002159"
     CDS             complement(13070..14005)
                     /locus_tag="IYF54_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832477.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3895 a         3162 c         2478 g         4558 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaggagcaa tcgctttttt attgatcacc atttttcttt taggtctatg gtgtcgggca
      181 ctgcgggtct tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa
      241 gttctcttct taaaacaaac ttaattaatt aaattatact tttaattctc aagaaacaca
      301 aactaatccg aagaattctt tgttttgtca tttaaatgta acatagccga aaacgaaaag
      361 taatattgat ttattcttct aacacataaa gaacagctgt ctccaacttg cagacagctg
      421 ttcttatcag tcaaacaatt gcttggatct atgatagttg attgtacgta ttcacgacat
      481 tttctactgt aaatccaaat tcttctaaaa ttttagtgcc aggggcagat gcaccgaaat
      541 gatcgattgt aatcgttgcg ccctctgttc ctacgtagcg ttcccaacca aatgatgcag
      601 cagcttcgat agccacacgt ttcttcactg atttaggaag tacactttct ttatattcag
      661 aagattgttt ttcaaagagg tcaaagcttg gcatagaaac aacagaaaca tctttgcctt
      721 tttcagccaa ttctttttga gctttcacag ccaaatcaac ttcagaacct gttgcaatca
      781 aaataccttc tggtgtttct ccttgtgatg gagaaagaac atacgcgcct tttttcacca
      841 tttcatctgc tacttctttt gtagaaggca atactggaag gttttgacga ctcaatacaa
      901 ggatagtagg cgcatctgtt gtctccatcg ctactttcca agcagcacgt gtttcatttc
      961 cgtctgccgg acgaatcact tgaacaccag gcatgcagcg aacagatgct agttgttcaa
     1021 tcggttcgtg cgttggtccg tcttctccga cagcaaccga gtcatgtgtt aagacgtaaa
     1081 caacaggagt atgttggatt gctgctaaac gaactgctgg tcttagataa tcgacaaaga
     1141 caaagaatgt tccaccgtag atacgagttc cgccatgaag ttggatacca ttcatagccg
     1201 aagccattgc aaattcgcgg acaccgaacc agatgttgcg tccttcataa tgttcaggag
     1261 tgaaatcttt atctgcagcc accattgtat tattggaacc tgacaagtcc gctgaaccac
     1321 cccagaagct tggtatagct ttcgataatt cttggatgac ttctttactt gaaacacggc
     1381 ttgcttgact gcttccaact tcataagttg gcaattctgc atcccagttt tctggtagtt
     1441 tgccatcaaa agcatcttca aattgttgtg ctaattcagg ataagctttt ttataattgg
     1501 caaacatttc attccatgca tcttcagctt tttgtccttc ttctatcatc gtttgatgga
     1561 agcgggcagc tacttcttct ggaacagtga aatctggata ctcccaacca taaacttctt
     1621 tagccatctt gatgccatct tcaccaatag gagcaccatg aacagcagat gttccttctt
     1681 taggagcacc atagccgatg actgttttta cttcgatcaa tgttggtttg tctgtttcag
     1741 cttttgcttc ttcaatcgct ttagagattg cttctaggtc atttccgtct ttcactaaga
     1801 tatgttgcca gccatatgct tcatagcgag caccaacgtt ttctgtgaaa gcttttgaag
     1861 ttggaccgtc tagtgaaata tcgtttgaat cgtataaaac gatcaatttc cctagtttca
     1921 tatgacctgc catcgaactt gcttcttgag ataccccttc catcaagtct ccgtcaccgc
     1981 atattgcata cgtatagtga tccatcacat taaagttttc tttgttatac gttgctgcaa
     2041 ggtgcgcttc tgccattgcc ataccgacag ccattgcaat cccttgtcct aatggccctg
     2101 tagtggcttc tacaccatct gtgtgatgta cttcaggatg tcctggtgtt ttagaatccc
     2161 attgacggaa ttgtttcaaa tcatcgattg tcacttgata gccagctaaa tgaagcaagc
     2221 tatacaacat tgctgaccca tgtcctgccg aaagaacgaa acggtcgcgg tcagcccagt
     2281 tttttgatgt cgttggattt actttcaggt gttttgtcca taacgcatat gccataggtg
     2341 ctgcgcccat cggcaatcca ggatgtcctg agtttgcttt ttggactgct tcaatactta
     2401 atgtacgaat tgtgttaacg ccaagttggt caattttgtc aaacaaaata acccctccta
     2461 ttaatttagg ttttttttac ctttatttct tacactttca ttctataaga tgtcacacga
     2521 aaaagcaatc tttttcagta ttttttacta aaagttgtca tttcggttgt gtaagccttt
     2581 tttcttttgg atttctttta atttttccgg ggtcacgtca ttgccctcag gatcgacgac
     2641 tttcattcct tcgatatgat gacgcatacc ggaacgaaaa gcttccaaat actctttgcg
     2701 taagttgatt tgttcttgtt cttcggcttt tgagagacca ttttccttct ttttacgtgc
     2761 aagttcattg atcctctcta ttttttcagg tgacagcatg ctcagccctc cttttactct
     2821 ccttcatatt atataaaaac tgaaaaaaaa acaactagat aaactaaata aaatacgaac
     2881 attcgtttga tttagcgctt tttatatggt actatttatt tatcaagtga aagaaggtgt
     2941 accttgtggc ccgacaaact gaatcaagac aaatcgaagt cttacgatat atccatgaac
     3001 aagtaaccca aaaaggatac ccgccgactg tccgtgaaat cggggaagct gttcagcttt
     3061 cttctacttc tactgttcac ggtcatcttg cacggcttga aaaaaaaggg tttatccaaa
     3121 gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca aggtttagaa aagattggga
     3181 ttcgtccaac aactattcca gtacttggcg tcgtaacagc tggagaaccc attttagcag
     3241 tagaggatgc ttcaaacttc tttccaatcc ctcctaatct ttcttccgaa gaaggcagtc
     3301 tctttatgct gacaattcgt ggagatagta tgatcaatgc tgggatcttg gatggtgaca
     3361 acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga tattgtcatt gcaatgactg
     3421 ccgaggacga agcgacttgc aaacgattct ataaagaaaa aaactttttc cgtctgcagc
     3481 ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt atctatatta ggacgagtag
     3541 taggacttta tcgtagtcat atctattaat tgctactaat aaaagaaaac tttttaaaaa
     3601 gagtttgaga cgatggtctc aaactctttt ttcgcccggg ttcttcttct ttcaaatttt
     3661 tatcacttcc ctctttacaa accgaacaaa tattcgtata ctaaatatac gaacaattgt
     3721 tcgcataaat taacgaggtg agaattcatg aaatcaatga gacgtcttgg cttagtatta
     3781 actgttttgt ttttaggtat tattattggt aatttcggca tacgtccagc tttacttgtg
     3841 tttgttccgt tatttacttt gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct
     3901 gagcggcgca gaattcatga agatcatttc tatcatgagc cctactacag atccaggcaa
     3961 tcataataag gattgactgc taattattca aaagacaaga agttaaactt ttacgggggt
     4021 gtgacaaaaa tcggtcacat ccttttttcg aataaacggt gttctaaagc cagtttttcg
     4081 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
     4141 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
     4201 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
     4261 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
     4321 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
     4381 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
     4441 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
     4501 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
     4561 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
     4621 attcgttttt cttttgcata atttacgatt tgcttcgtag cttcccgata agcgtcacca
     4681 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     4741 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     4801 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgccg
     4861 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     4921 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     4981 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     5041 ttcttgagaa tggatcagtt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     5101 caaataggcc tcatcaggcg aaatccccat cagataaaca cgattgaatg aataagaccg
     5161 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataataacta gagagttcgg
     5221 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     5281 ctggataatc tcaggcacga atttcaacgt agaagaatca aatgctaaat aaaggctacc
     5341 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     5401 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     5461 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     5521 accaaaagcg accgcatcaa gctgactcga tgcattggaa aggacaaatt tcagatgctg
     5581 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     5641 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     5701 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     5761 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     5821 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     5881 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     5941 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     6001 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     6061 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     6121 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     6181 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     6241 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     6301 cgcctgaagt cctatccgtt ctgaatgatg aatggcattg attcctaatg aaacaaggat
     6361 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     6421 aaattctaca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     6481 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     6541 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     6601 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     6661 gattccttct tcgatctttt cttggtatac agattggttt ggtccatacc catgctcaaa
     6721 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     6781 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     6841 gattacagct tcttggattc ggctgattgc tttatccata tcgtgcatca gatacggatc
     6901 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     6961 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     7021 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     7081 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     7141 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     7201 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     7261 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     7321 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     7381 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     7441 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     7501 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     7561 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     7621 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     7681 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     7741 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     7801 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     7861 atcagcctta tcgaagaaat ctgtctcgat tggacctgga ttcactgtcg taacagctac
     7921 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     7981 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagacg cgatattaat
     8041 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     8101 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     8161 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     8221 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     8281 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     8341 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     8401 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     8461 tcctcgcgaa atggaatctc aatgctatca aaatctttca tgatcgtcgt gttttcaaac
     8521 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     8581 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     8641 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     8701 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     8761 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     8821 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     8881 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     8941 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     9001 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     9061 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     9121 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     9181 aaaatatgat cgccgtgtaa atgtgtaata aaaattttac cgatttttct cggacgaata
     9241 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     9301 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     9361 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     9421 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     9481 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     9541 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     9601 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     9661 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcgg gtaatttctc
     9721 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     9781 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     9841 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     9901 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     9961 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
    10021 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
    10081 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
    10141 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
    10201 tgtaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
    10261 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
    10321 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
    10381 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
    10441 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
    10501 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
    10561 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
    10621 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
    10681 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
    10741 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
    10801 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
    10861 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
    10921 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
    10981 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
    11041 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggg
    11101 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
    11161 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
    11221 tctagttcga tttttcgctc ttgtcctggt tcgccgtttt cagcaatttc cggtgcagga
    11281 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
    11341 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
    11401 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
    11461 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
    11521 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
    11581 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
    11641 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
    11701 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
    11761 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
    11821 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
    11881 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
    11941 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
    12001 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
    12061 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
    12121 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
    12181 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
    12241 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
    12301 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
    12361 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
    12421 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
    12481 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
    12541 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
    12601 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
    12661 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
    12721 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
    12781 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
    12841 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
    12901 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
    12961 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
    13021 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
    13081 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
    13141 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
    13201 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
    13261 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
    13321 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
    13381 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
    13441 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
    13501 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
    13561 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
    13621 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    13681 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    13741 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    13801 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    13861 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    13921 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    13981 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    14041 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DACIWG010000061        14075 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.065, whole genome shotgun sequence.
ACCESSION   DACIWG010000061 DACIWG010000000
VERSION     DACIWG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.065"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..587)
                     /locus_tag="IYF54_002160"
     CDS             complement(<1..587)
                     /locus_tag="IYF54_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832478.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IYF54_002161"
     CDS             complement(735..1277)
                     /locus_tag="IYF54_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ9832479.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IYF54_002162"
     CDS             1412..1801
                     /locus_tag="IYF54_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832480.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IYF54_002163"
     CDS             1805..2569
                     /locus_tag="IYF54_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9832481.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="IYF54_002164"
     CDS             2698..3645
                     /locus_tag="IYF54_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ9832482.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="IYF54_002165"
     CDS             3775..4515
                     /locus_tag="IYF54_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832483.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="IYF54_002166"
     CDS             complement(4806..5474)
                     /locus_tag="IYF54_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9832484.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="IYF54_002167"
     CDS             5608..5895
                     /locus_tag="IYF54_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832485.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="IYF54_002168"
     CDS             6311..7849
                     /locus_tag="IYF54_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ9832486.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="IYF54_002169"
     CDS             7846..8811
                     /locus_tag="IYF54_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832487.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="IYF54_002170"
     CDS             8798..10120
                     /locus_tag="IYF54_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9832488.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="IYF54_002171"
     CDS             10361..11104
                     /locus_tag="IYF54_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832489.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="IYF54_002172"
     CDS             11119..12111
                     /locus_tag="IYF54_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ9832490.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="IYF54_002173"
     CDS             12123..12860
                     /locus_tag="IYF54_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832491.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="IYF54_002174"
     CDS             12850..13572
                     /locus_tag="IYF54_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ9832492.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13656..>14075)
                     /locus_tag="IYF54_002175"
     CDS             complement(13656..>14075)
                     /locus_tag="IYF54_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ9832493.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4713 a         2387 c         2912 g         4063 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgaggtc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    13681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    13741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    13801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    13861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    13921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    13981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    14041 ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DACIWG010000062        13948 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.066, whole genome shotgun sequence.
ACCESSION   DACIWG010000062 DACIWG010000000
VERSION     DACIWG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.066"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            950..1423
                     /locus_tag="IYF54_002176"
     CDS             950..1423
                     /locus_tag="IYF54_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ9832494.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="IYF54_002177"
     CDS             1432..1659
                     /locus_tag="IYF54_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832495.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="IYF54_002178"
     CDS             2295..2666
                     /locus_tag="IYF54_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832496.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="IYF54_002179"
     CDS             complement(2922..3164)
                     /locus_tag="IYF54_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9832497.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="IYF54_002180"
     CDS             complement(3196..4098)
                     /locus_tag="IYF54_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9832498.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="IYF54_002181"
     CDS             complement(4111..4299)
                     /locus_tag="IYF54_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ9832499.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IYF54_002182"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="IYF54_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ9832500.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="IYF54_002183"
     CDS             complement(4914..5807)
                     /locus_tag="IYF54_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9832501.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6823)
                     /locus_tag="IYF54_002184"
     CDS             complement(5885..6823)
                     /locus_tag="IYF54_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ9832502.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="IYF54_002185"
     CDS             complement(6857..7207)
                     /locus_tag="IYF54_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9832503.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="IYF54_002186"
     CDS             complement(7240..8142)
                     /locus_tag="IYF54_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ9832504.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="IYF54_002187"
     CDS             complement(8135..8992)
                     /locus_tag="IYF54_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ9832505.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="IYF54_002188"
     CDS             9326..10135
                     /locus_tag="IYF54_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832506.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="IYF54_002189"
     CDS             10175..10672
                     /locus_tag="IYF54_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9832507.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="IYF54_002190"
     tRNA            complement(11057..11130)
                     /locus_tag="IYF54_002190"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="IYF54_002191"
     CDS             11317..11640
                     /locus_tag="IYF54_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832508.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="IYF54_002192"
     CDS             11804..12058
                     /locus_tag="IYF54_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832509.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="IYF54_002193"
     CDS             12128..12373
                     /locus_tag="IYF54_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832510.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="IYF54_002194"
     CDS             12449..12814
                     /locus_tag="IYF54_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832511.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="IYF54_002195"
     CDS             complement(12875..13438)
                     /locus_tag="IYF54_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832512.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4090 a         2577 c         2304 g         4977 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc aactttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatgaatt
//
LOCUS       DACIWG010000063        13925 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.067, whole genome shotgun sequence.
ACCESSION   DACIWG010000063 DACIWG010000000
VERSION     DACIWG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.067"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            131..505
                     /locus_tag="IYF54_002196"
     CDS             131..505
                     /locus_tag="IYF54_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ9832513.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IYF54_002197"
     CDS             complement(619..1248)
                     /locus_tag="IYF54_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9832514.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IYF54_002198"
     tRNA            complement(1382..1454)
                     /locus_tag="IYF54_002198"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IYF54_002199"
     tRNA            1668..1741
                     /locus_tag="IYF54_002199"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IYF54_002200"
     CDS             1696..1845
                     /locus_tag="IYF54_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832515.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IYF54_002201"
     CDS             2338..3666
                     /locus_tag="IYF54_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ9832516.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IYF54_002202"
     CDS             3868..4701
                     /locus_tag="IYF54_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ9832517.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IYF54_002203"
     CDS             4717..5454
                     /locus_tag="IYF54_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ9832518.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IYF54_002204"
     CDS             complement(5609..6577)
                     /locus_tag="IYF54_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ9832519.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IYF54_002205"
     CDS             6809..7642
                     /locus_tag="IYF54_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ9832520.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IYF54_002206"
     CDS             7676..8515
                     /locus_tag="IYF54_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832521.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IYF54_002207"
     CDS             8541..9101
                     /locus_tag="IYF54_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ9832522.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IYF54_002208"
     CDS             9300..11021
                     /locus_tag="IYF54_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ9832523.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IYF54_002209"
     CDS             11186..12328
                     /locus_tag="IYF54_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ9832524.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IYF54_002210"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IYF54_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ9832525.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4756 a         2299 c         2835 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaag
//
LOCUS       DACIWG010000064        13161 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.068, whole genome shotgun sequence.
ACCESSION   DACIWG010000064 DACIWG010000000
VERSION     DACIWG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.068"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(285..2012)
                     /locus_tag="IYF54_002211"
     CDS             complement(285..2012)
                     /locus_tag="IYF54_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9832526.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2005..3198)
                     /locus_tag="IYF54_002212"
     CDS             complement(2005..3198)
                     /locus_tag="IYF54_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ9832527.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3385..4029)
                     /locus_tag="IYF54_002213"
     CDS             complement(3385..4029)
                     /locus_tag="IYF54_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9832528.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4123..4257)
                     /locus_tag="IYF54_002214"
     CDS             complement(4123..4257)
                     /locus_tag="IYF54_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ9832529.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4259..6391)
                     /gene="feoB"
                     /locus_tag="IYF54_002215"
     CDS             complement(4259..6391)
                     /gene="feoB"
                     /locus_tag="IYF54_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ9832530.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6388..6861)
                     /locus_tag="IYF54_002216"
     CDS             complement(6388..6861)
                     /locus_tag="IYF54_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ9832531.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7262..7486
                     /gene="nrdH"
                     /locus_tag="IYF54_002217"
     CDS             7262..7486
                     /gene="nrdH"
                     /locus_tag="IYF54_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ9832532.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7645..9804
                     /gene="nrdE"
                     /locus_tag="IYF54_002218"
     CDS             7645..9804
                     /gene="nrdE"
                     /locus_tag="IYF54_002218"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ9832533.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9854..10819
                     /gene="nrdF"
                     /locus_tag="IYF54_002219"
     CDS             9854..10819
                     /gene="nrdF"
                     /locus_tag="IYF54_002219"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ9832534.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10908..12047)
                     /locus_tag="IYF54_002220"
     CDS             complement(10908..12047)
                     /locus_tag="IYF54_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ9832535.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12356..13069)
                     /locus_tag="IYF54_002221"
     CDS             complement(12356..13069)
                     /locus_tag="IYF54_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ9832536.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
BASE COUNT         4044 a         2732 c         2307 g         4078 t
ORIGIN      
        1 ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga gaatgcactt
       61 cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc tctttgactc
      121 gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga tgagctacgc
      181 attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca aatttggata
      241 tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt tttccagctt
      301 tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg atcttttcca
      361 ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa attgtctaat
      421 actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt atggatctgc
      481 tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata tttggcaaac
      541 atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat cgcttgacct
      601 agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt caagttgtca
      661 tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc gattcctatg
      721 attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat gtaggctggc
      781 actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac ttgatcttta
      841 tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat ggcatctgac
      901 tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat cgttacatca
      961 tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc tgtatcccat
     1021 tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact ccctatacca
     1081 atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg ttttttactt
     1141 ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg ctgtgtaaaa
     1201 cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc attcccgact
     1261 tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc gatcatagag
     1321 gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc catgatcgta
     1381 cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct cccgaatgaa
     1441 tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt aaaacgaacg
     1501 gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc gataccgaaa
     1561 aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa gtcaaggatc
     1621 ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa tggtacccca
     1681 atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat caaatcattc
     1741 catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt aagtcctgga
     1801 cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa aggagctgga
     1861 atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt ttgtaaaaat
     1921 gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac ccagtttgcc
     1981 actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg tttctgctaa
     2041 tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct ctacaactgc
     2101 aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca tgtctttccc
     2161 aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct tcctagcttc
     2221 taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa cagccatcag
     2281 catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat cagattcgat
     2341 attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg aacgatcgac
     2401 ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt cttcacctgt
     2461 tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa tccgtaaagc
     2521 ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt gcaggtccag
     2581 taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg cttcgtccat
     2641 cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt gttgcatccc
     2701 accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat tttctaatgc
     2761 tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta acccgtattc
     2821 tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa ccatattgat
     2881 tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat cttctccatc
     2941 aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga tcaatgtgga
     3001 tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta cgtcaaaatt
     3061 cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt tcggctggtg
     3121 atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg ttaaatgttt
     3181 tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt tctttctgaa
     3241 aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga aagaaatttg
     3301 cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt agccaagtta
     3361 atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt aaaaaaagtt
     3421 tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc ctgccgtaaa
     3481 tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg cgataactgt
     3541 ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat gcattgcttt
     3601 ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc gcttcgtttg
     3661 attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt gcttctccac
     3721 acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat attgcaatag
     3781 gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc gtacttccat
     3841 gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag aagcattgta
     3901 cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc ggttttcaac
     3961 gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga tttttttctt
     4021 tccagacatt ttctttcctc tttctttttt tactttccct catcttacta tgctctagca
     4081 aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct tgatctgttc
     4141 cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt ttaaacgagt
     4201 atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg tcgccatcct
     4261 attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt ggctgacgga
     4321 acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct aacgctacct
     4381 gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa taagctaaac
     4441 cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc cgatggatag
     4501 ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat gaataagctg
     4561 ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt tcggatactt
     4621 ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg accaatcctg
     4681 taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa acaactgcca
     4741 ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga gcataaaaat
     4801 tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt cctgcttttt
     4861 taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat tgaggaaaat
     4921 gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa cgtgtttttt
     4981 tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca gaaggagcta
     5041 cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc aactttgcag
     5101 aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg ttttcaatcg
     5161 tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata ggaatgaagg
     5221 attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg atcctcgcca
     5281 tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat tgcggtagaa
     5341 atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc tgcatccatc
     5401 ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat gtaccaaaca
     5461 atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc agataataga
     5521 tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt gcaatctgat
     5581 cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag agtgtacata
     5641 gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg tcgtccaata
     5701 gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact gctatctcgt
     5761 aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag cgtttcttgc
     5821 cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa cgtggatcat
     5881 atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc ttttcgatta
     5941 gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca ccaagaccat
     6001 aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc atcatattca
     6061 gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa agatttcgtt
     6121 ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga accaaataat
     6181 ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg ggtaggtcct
     6241 gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact gttacgcccg
     6301 gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta gttttcccgc
     6361 tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta tcaagattag
     6421 ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt cgattggatc
     6481 tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc ccatatccat
     6541 taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag tctctcctgc
     6601 ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat tgacaaagat
     6661 ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta aaatggcatt
     6721 gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga tattttgcaa
     6781 atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga aattctgctt
     6841 tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat gtattaaaat
     6901 cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa tcattctcac
     6961 atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg agcgaatctt
     7021 tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca caattttttc
     7081 tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt tcaaaaacac
     7141 aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt ttgacttttt
     7201 tactaagagt tttatactat ctattataga taataagaat gtgttagagg aggcactcac
     7261 catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt
     7321 tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt
     7381 agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac
     7441 agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa
     7501 tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt
     7561 ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat
     7621 acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc
     7681 aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa
     7741 gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttccatct
     7801 attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa
     7861 ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct
     7921 ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac
     7981 ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg
     8041 aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag
     8101 ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt
     8161 ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg
     8221 caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc
     8281 gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc
     8341 gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat
     8401 ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat
     8461 gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa
     8521 ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat
     8581 ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca
     8641 aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa
     8701 caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc
     8761 gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct
     8821 gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg taacttaggc
     8881 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
     8941 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
     9001 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
     9061 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
     9121 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
     9181 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
     9241 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
     9301 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
     9361 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
     9421 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
     9481 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
     9541 atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt cgatcaaggc
     9601 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
     9661 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
     9721 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
     9781 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
     9841 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
     9901 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
     9961 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
    10021 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
    10081 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
    10141 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
    10201 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
    10261 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
    10321 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
    10381 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
    10441 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
    10501 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
    10561 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
    10621 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
    10681 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
    10741 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
    10801 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
    10861 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
    10921 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
    10981 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
    11041 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
    11101 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
    11161 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
    11221 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
    11281 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
    11341 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
    11401 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
    11461 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
    11521 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
    11581 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
    11641 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
    11701 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
    11761 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
    11821 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
    11881 taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat tttcaatatt
    11941 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
    12001 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
    12061 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
    12121 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
    12181 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
    12241 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
    12301 atttggctgg gcttattact tgatgtagga catctatttt acaacctcag atcatttatt
    12361 gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca
    12421 catcggcata agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt
    12481 agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa
    12541 aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt
    12601 tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa
    12661 aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg
    12721 cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc
    12781 ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac
    12841 gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct
    12901 caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac
    12961 ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga
    13021 tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct
    13081 ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta
    13141 cgaaaaaagg actgtgacaa a
//
LOCUS       DACIWG010000065        13147 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.069, whole genome shotgun sequence.
ACCESSION   DACIWG010000065 DACIWG010000000
VERSION     DACIWG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.069"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(77..149)
                     /locus_tag="IYF54_002222"
     tRNA            complement(77..149)
                     /locus_tag="IYF54_002222"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IYF54_002223"
     CDS             530..1099
                     /locus_tag="IYF54_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ9832537.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IYF54_002224"
     CDS             1086..1946
                     /locus_tag="IYF54_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9832538.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IYF54_002225"
     CDS             2136..2840
                     /locus_tag="IYF54_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832539.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IYF54_002226"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IYF54_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ9832540.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IYF54_002227"
     CDS             4677..5993
                     /locus_tag="IYF54_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832541.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IYF54_002228"
     CDS             5994..6863
                     /locus_tag="IYF54_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832542.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IYF54_002229"
     CDS             6918..7727
                     /locus_tag="IYF54_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ9832543.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IYF54_002230"
     CDS             complement(7830..8459)
                     /locus_tag="IYF54_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ9832544.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IYF54_002231"
     CDS             8629..9921
                     /locus_tag="IYF54_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9832545.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IYF54_002232"
     CDS             complement(9997..10659)
                     /locus_tag="IYF54_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9832546.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IYF54_002233"
     CDS             10884..11168
                     /locus_tag="IYF54_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ9832547.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IYF54_002234"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IYF54_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ9832548.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACIWG010000066        13087 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.070, whole genome shotgun sequence.
ACCESSION   DACIWG010000066 DACIWG010000000
VERSION     DACIWG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.070"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(80..2200)
                     /locus_tag="IYF54_002235"
     CDS             complement(80..2200)
                     /locus_tag="IYF54_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9832549.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2430..3608
                     /locus_tag="IYF54_002236"
     CDS             2430..3608
                     /locus_tag="IYF54_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ9832550.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="IYF54_002237"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="IYF54_002237"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ9832551.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4405..4890
                     /gene="ybaK"
                     /locus_tag="IYF54_002238"
     CDS             4405..4890
                     /gene="ybaK"
                     /locus_tag="IYF54_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ9832552.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4961..6214)
                     /locus_tag="IYF54_002239"
     CDS             complement(4961..6214)
                     /locus_tag="IYF54_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9832553.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6330..7679
                     /gene="gdhA"
                     /locus_tag="IYF54_002240"
     CDS             6330..7679
                     /gene="gdhA"
                     /locus_tag="IYF54_002240"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ9832554.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7791..9137
                     /locus_tag="IYF54_002241"
     CDS             7791..9137
                     /locus_tag="IYF54_002241"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ9832555.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9445..9831)
                     /locus_tag="IYF54_002242"
     CDS             complement(9445..9831)
                     /locus_tag="IYF54_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ9832556.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10710..10922
                     /locus_tag="IYF54_002243"
     CDS             10710..10922
                     /locus_tag="IYF54_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832557.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11200..12999)
                     /locus_tag="IYF54_002244"
     CDS             complement(11200..12999)
                     /locus_tag="IYF54_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ9832558.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4292 a         2354 c         2577 g         3864 t
ORIGIN      
        1 attattctaa gatttctctt taccaacatc atttgacaaa gagcctaatt ttgtcgctgc
       61 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      121 acgtttgcgt tttataaaga tcaacagttg attgtccctt gtgttcacta aagacactag
      181 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      241 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      301 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      361 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      421 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      481 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      541 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      601 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      661 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      721 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      781 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      841 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
      901 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
      961 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1021 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1081 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1141 cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1201 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1261 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1321 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcaagctgg agaccattct
     1381 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1441 cattttttcc tttagcgagc ccgaacaaat catcatttgg tgctgcataa tgttctttta
     1501 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1561 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1621 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1681 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1741 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1801 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1861 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     1921 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     1981 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2041 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2101 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacaac tagtaaagaa aaaaatccga
     2161 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2221 ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt
     2281 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2341 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2401 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2461 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2521 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2581 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2641 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2701 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2761 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2821 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg
     2881 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     2941 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3001 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3061 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3121 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3181 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3241 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3301 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3361 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3421 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3481 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3541 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3601 accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta
     3661 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3721 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3781 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3841 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     3901 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     3961 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4021 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4081 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4141 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4201 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg
     4261 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4321 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4381 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4441 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4501 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctca tatatttaaa
     4561 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4621 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4681 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4741 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4801 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4861 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     4921 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     4981 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5041 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5101 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5161 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5221 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5281 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5341 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5401 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5461 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5521 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5581 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5641 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5701 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5761 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5821 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     5881 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     5941 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6001 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6061 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6121 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6181 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6241 tatctgaaga aagaaaacag aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
     6301 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
     6361 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
     6421 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
     6481 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
     6541 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
     6601 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
     6661 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
     6721 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
     6781 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
     6841 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
     6901 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
     6961 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
     7021 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
     7081 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
     7141 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
     7201 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
     7261 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
     7321 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
     7381 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
     7441 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgcccagc
     7501 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
     7561 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
     7621 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
     7681 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
     7741 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
     7801 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
     7861 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
     7921 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
     7981 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
     8041 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
     8101 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
     8161 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
     8221 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
     8281 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
     8341 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
     8401 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
     8461 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
     8521 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
     8581 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
     8641 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
     8701 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
     8761 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
     8821 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
     8881 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
     8941 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
     9001 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
     9061 acaagaaaaa catgttcgca cttctaggca aaccgggctt tgaagatctt gctaaagaat
     9121 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
     9181 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
     9241 attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg
     9301 attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga
     9361 aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt
     9421 gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg
     9481 taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg
     9541 caagatcgtt ccttcggtgt aagcagaacc attggcagag tcaccagaag taatgacatc
     9601 gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc
     9661 tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt
     9721 gactaccgca taataatctt tacctacata agtagactga tagtagtccc aacctttcca
     9781 tcctataaaa gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct
     9841 ccttttttat ttttattata ttaaatatta tacagataat aacggaatat tatcatcgaa
     9901 acagctaaca aaattgtcat aaaaaacgag atggttgata ttagaacttg tgtttttgtt
     9961 taggtctaga gataaagaga gacaatttcc aaatatatgt aatgaataca aaaagacgtt
    10021 tgtcgcggat taccgttaca aacgtctttt attgattttc cgagatctca tcagtcgcca
    10081 tttggaatgg ttcactcctt atctagattc gtttaatgct cggactgctt gttcgattgt
    10141 aataccaaac gcaacgttat tttcgttttg tgcatcaatc gccataaaac ggttagaagt
    10201 tgtatcaaaa gctacgcggt attgcatatt tgtttttgta aacgtcataa aattcatctc
    10261 ctttgtaatg taatttagtt cttgaaagtt tcctttaaac tttcgaactt ataccaagtt
    10321 tagcatcgta cgttttttga tgcaattaaa aagcatttat cattttcctt atttattttt
    10381 tcttaaatgg tttagctatt ttcttttaat ccgataagta gcccaatcat caaggttaca
    10441 aatactattg cttgaatata aatcatatgc tcacctcttt ataaataact tagcaatata
    10501 aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca caacgtctaa
    10561 gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaaaataa aaaataaaaa
    10621 aatgaataaa aacgttttca aatacaaggt aatttgttat tataaaataa agaaaaaaca
    10681 aaataaaaat aaagaaaaga gggaatgaaa tggtaaaagc agaagtagga agatcttata
    10741 tatgcaaacc gatcggagta tctcatgaac taatcggctg tattgaacgt acatatatca
    10801 acacggctgt cgtgtatgtt gaaagttatg accaacgaga ctatcaactt atcaatgaaa
    10861 gcaagtatcg catgctcgta aaattctgtg atattagcgc acctactgaa ttggtttctt
    10921 aaacagtatt tttgagaatt cgcaagaact cccttataat ataaataaag acagaaggga
    10981 gcgagaagat atgccaaaaa gaaaaagttc agggaaaatg ggaattattc ttgcagtgat
    11041 ctctgcaatt cctgctattt tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta
    11101 agcacacatt atgagcgtca aacaaaaaac gcatatacct gcggtggcag gttatgcgtt
    11161 ttttgttgta taaagacttt tttcattaac gatttttgat tagccgactc ccaaaacaaa
    11221 atttagcagt ttatctgaat aagtgatttc ttcatctgtt tgtttcacac tttcatttag
    11281 tagtcccatc tcatcggtta tgataccatc cacaccataa aacatcatgc gactcatggc
    11341 atctgaatcg ttgattgtcc atacatatac ttcttttccg tcttggtggg cagaattaat
    11401 gaagttacgg ttgatcgtac tatattccat tgtcagaaaa tcagcaggcg tcacaggggg
    11461 cccaacaata ttgaaaggta agatgtagcc cacataaaag tttggttctg cttcttttaa
    11521 atctgaaacg gtttggtacg tcaaactttg caaaatatga tggttctcta agataacttt
    11581 gcggtattta tggacaaatc gttcgacaag gtcatccgaa tcttttttag aggtcttgat
    11641 ctcaataagt aatttttggt ttagcttatg tgctttttcc aaataagaat caaaagaaca
    11701 aacaggggct tgcactccgt tttcagtaac tgtaagattt gtcaattctt tcaatgtaag
    11761 atcctgtggt gttccattta cccctgttaa gtgacgaaga tttgcatcat gcatcactac
    11821 gaaatgacca tcttttgttt cctgaacatc catctctaca taatcaggat gatagtctct
    11881 gcttgttttt tcaagtgctt ctaaagtatt ttgcgcccca ttttgggtac tcacacctct
    11941 gtgagagaca gtcactggtt ttgtctgaac agctgaagtc agataatcca tattgtaaag
    12001 acagacgcca ataccgaaaa aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga
    12061 gaaacgcaag ttgggggctt gtgggataaa ccacttaggg atctcaggca agaagccttc
    12121 gtcatccatg aaatcaacaa tgatataaaa aatccctacg gtagacatca cgatattgaa
    12181 taataaaatc ccttgtaaaa gcgtcatagc aaacacagcg ctgatcaatg catagtctgg
    12241 gaaaaattgt tcaaccatac tttgtgccaa aataacaacg atatatccta aagaagaaag
    12301 gagtaaaatc gtaccgccaa tcacaataaa ttggcccgtg atggccaaaa gacgcgattt
    12361 ggtcaatgac cagctttccc gaatggctgc tcgaaaagaa cgatctcgta gaatcatttc
    12421 cggtaaagca aaaatcagtc gaatcccaat atagccaagg aaaatataaa cgagtaaaaa
    12481 ggaactgacg atgatccaac gattagcaaa aataaaatcc ataatgaaag cggggatttt
    12541 tatttttgac agtagatcag aattaaaact taatccgctg atcggtagaa tcaataaaaa
    12601 ataagcaagg aaaaacaaaa aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa
    12661 gtgtaagagt tgcttcaaag agattggttc tttcttttta ataaaaaaaa cactcatcaa
    12721 aagaaaagta aattcgaaat aaactaacag taaaatcgtc atcaaaacta cgattaacga
    12781 gagtagaacc ccaggatgtt gagaaaaaat tgaaggaata ttgtcataag acaaatagtc
    12841 aattgcccct cggtgtaaaa taaaacgggt cgtactgctc aataagggta aacaaataaa
    12901 taaaataaag ccatgcatca gcaagacatc acggaaataa gcttgtgttc cttttaaaaa
    12961 gtcccaagca ttacctaagc tacgtttcaa ataattcatc ctatcacctg ctcttcacat
    13021 tgtagcgcaa atctaatgaa aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag
    13081 atttaac
//
LOCUS       DACIWG010000067        12938 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.071, whole genome shotgun sequence.
ACCESSION   DACIWG010000067 DACIWG010000000
VERSION     DACIWG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.071"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            365..2737
                     /locus_tag="IYF54_002245"
     CDS             365..2737
                     /locus_tag="IYF54_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832559.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2798..4258
                     /locus_tag="IYF54_002246"
     CDS             2798..4258
                     /locus_tag="IYF54_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ9832560.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            4269..4712
                     /locus_tag="IYF54_002247"
     CDS             4269..4712
                     /locus_tag="IYF54_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ9832561.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQ"
     gene            4797..6473
                     /locus_tag="IYF54_002248"
     CDS             4797..6473
                     /locus_tag="IYF54_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ9832562.1"
                     /translation="MNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQ
                     KEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVETE
                     KKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAIQGLYEGKYLSYPRTDTPYITEGEY
                     AYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLS
                     PLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPLDLGWKKLFGKES
                     DGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEA
                     QSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSA
                     EMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAAS
                     EKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQN
                     SRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            6719..7315
                     /locus_tag="IYF54_002249"
     CDS             6719..7315
                     /locus_tag="IYF54_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9832563.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            7328..8227
                     /locus_tag="IYF54_002250"
     CDS             7328..8227
                     /locus_tag="IYF54_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ9832564.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            8230..8714
                     /gene="ssb"
                     /locus_tag="IYF54_002251"
                     /pseudo
     CDS             8230..8714
                     /gene="ssb"
                     /locus_tag="IYF54_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8736..8993
                     /locus_tag="IYF54_002252"
     CDS             8736..8993
                     /locus_tag="IYF54_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832565.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(9152..9286)
                     /locus_tag="IYF54_002253"
     CDS             complement(9152..9286)
                     /locus_tag="IYF54_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ9832566.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            9429..9689
                     /locus_tag="IYF54_002254"
     CDS             9429..9689
                     /locus_tag="IYF54_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832567.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            10138..10344
                     /locus_tag="IYF54_002255"
     CDS             10138..10344
                     /locus_tag="IYF54_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ9832568.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            10344..10595
                     /locus_tag="IYF54_002256"
     CDS             10344..10595
                     /locus_tag="IYF54_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832569.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10611..11048
                     /locus_tag="IYF54_002257"
     CDS             10611..11048
                     /locus_tag="IYF54_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832570.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11484..11672
                     /locus_tag="IYF54_002258"
     CDS             11484..11672
                     /locus_tag="IYF54_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832571.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            11730..11996
                     /locus_tag="IYF54_002259"
     CDS             11730..11996
                     /locus_tag="IYF54_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832572.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            12006..12239
                     /locus_tag="IYF54_002260"
     CDS             12006..12239
                     /locus_tag="IYF54_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ9832573.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12582..12764
                     /locus_tag="IYF54_002261"
     CDS             12582..12764
                     /locus_tag="IYF54_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ9832574.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            12754..>12938
                     /locus_tag="IYF54_002262"
     CDS             12754..>12938
                     /locus_tag="IYF54_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832575.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         4786 a         2142 c         2713 g         3297 t
ORIGIN      
        1 tactgtggga tttcccagaa actcactgta tagccacaac ctcgcctaat agaatacggt
       61 gataaaagag ctagaagctg ccttccaaac tactatttga aagtctagtc cattatcgaa
      121 acagctaatg agttggtagt gataacagtg gttcgggtaa cagactgaac agggtagaca
      181 aacgctacag gaggaaatag caaattcccg ttggatcctt tccatacatc cattgtactc
      241 tgagaaatca cacaatatcg aaacaaattc cgttaggaat gtgttattca ataaatttta
      301 taatctagaa acattttgaa taaaaactat tagacaaatg atattttacg aggaggaaac
      361 ggacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
      421 attagtacga ttgcaaaatg aattaaatga tcaaatcgaa aagatgtttg aacaccaaag
      481 aaagaccaac ggtcaaccaa tgaacgataa gagaaacggg cattcttggt ttaggcaaca
      541 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaaaaaca
      601 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggttataaca agtacggtgg
      661 cctggatatg acgatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      721 aggtgaatcg actttttcag ctgccacgat ccgaaaatac caacgaaaat tggaaacatt
      781 agaacagctg aaagaaaaat cagagaaagg aaaagaaaat attcttcctg aagtacaagc
      841 gatcattgac agcggacgag tgacgcaatg gaaaaagaat ccaactatct attttctgaa
      901 aggctatcga aaagtcgctt tagaactcaa tgaaaacggg ggatttgagg aatctaaaaa
      961 ataccaagcc acgtcggaag aagaaaaggc agctgttcgt gaattacttg aagaagctac
     1021 acctactgat tctgtgaagg agaaagaaac cgatcatttc tatcgagtag aattcaacga
     1081 aaatgatcca gcaaaagggt tgaaaagtta tggtggggag atcgtaacac tagaattgat
     1141 cgatcaactc aaaacactgg ataaccaata cgccgcagaa gcaggctatt ataaattcta
     1201 ttttgaagaa gtgaagaatg gagaagtagt agatcagata cgaatggatc taggggatgg
     1261 cgtcaatagt aatcacccca tctatttaga gttagctaga ttgtgcgaac aagtacctga
     1321 gaaggaacaa aaacaaacag tcaaaaatga acctatttca aaagtacaaa atgagattaa
     1381 agagacaaaa acaaaacaaa agggttatca aaaacgaacg gcagaagaga ttaagcaaga
     1441 agtcaaatca ctttcccaac aggcgcttga tgcggtgaaa aagcatactc attcacctga
     1501 agacgtcaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1561 attattgatc gaggaacaat ttccaggcgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1621 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1681 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagagg ctacaccaga
     1741 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1801 tgaaaaagtg tttgatatca cacaaacaca attgaagcct gaagactatc cgaaaatctt
     1861 cccgaaccgc gtctttgatt ttcaattgga tcaaacaggg catacagaac ttcaagttgg
     1921 gattcaagcg gtagccaaaa atattggaat tcaaattcga gatatgacag aaagtgaagt
     1981 ctatcaaaga gaattaggac aagcacgtgg ggcgtacgtt cgtaacgaat ttactggcga
     2041 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcca caagtattca
     2101 cgaattagcc catgctagaa tgcacaaatt ttcagaatta gatactgcaa caaaagaact
     2161 acaggcagaa atgacctcct atattgtgtg caagcatttt gggatggaca cctcggagaa
     2221 agctattcct tatattgcga catggacgaa gaatggtcag acgttggatg ataaggaagc
     2281 tgcggaacgt gggaaaatca tgaatgatgt cagtcgtgtc gcaaatgaat tcattcaaac
     2341 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccagc cagcagaagt
     2401 gaagcaggat gagaaaaatg ttgtagaaac agcagaacaa aagaatattg tgctcgttgg
     2461 ctcactttgg atcgacaaga aagacgggaa agtcctggaa gaagacacgg gccgaacgtt
     2521 acatttgaag gatgcggcgt ttattgaaga tacgcagcat aatcaagtga ttttaaaagg
     2581 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2641 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2701 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2761 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgagaaaa aaatgagtct
     2821 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2881 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2941 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     3001 actttttttc atagacaaag agattcaaga ctctaagcaa caatttaaag cagccacaga
     3061 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     3121 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattattt
     3181 gaaggaagaa cgaaaactgc ccgattggtt gattgattat gcagaaaaag aaggattgat
     3241 cgccgaacta aagccaaaac atgaacggca aaatttctta gttcaagacg atcgtttgga
     3301 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3361 tcaaggaaca ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3421 taaaaattcg acagcgaatc atggatttaa cctaaagatc ggtgacccta aacagttaaa
     3481 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3541 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3601 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccattg agatttgtgt
     3661 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3721 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3781 aaaagaatac aaagtcattt atgaagaagt tgcaaaagaa atgaaagttg aacctgaagc
     3841 aattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3901 tcacaaagta aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagctat
     3961 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaaggttgtg aacgcgtgga
     4021 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     4081 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     4141 acctgaagta aaaaaggatt ggaatgatca attgaagcat gaaatccacc aacaagagat
     4201 cagaaagcaa aaacgtgcga tgttgtttca acaaggcaaa caacaagaaa gggagtgaaa
     4261 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     4321 atagcttctc taaagccacc cgaaaagacg ggtattttga aatccaagat agattgtttc
     4381 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcgcccgata
     4441 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cctacacatt
     4501 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4561 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatagctt
     4621 ggacgatcat tcaacaagca ggcgccgatc atggaaagat atttaaaaga ctttggatca
     4681 actccttaga aaaagaagcc atttatcagt gattccagca attacgagac gcagaagaga
     4741 cctatcccaa atttgaagaa gcacaagcac gccagatcgc tgattggttg atcggcatga
     4801 acggcagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agtttttcct
     4861 taggcagagt ccaaacccca acgctttaca tgatttatca gctgcaggaa aagattagaa
     4921 acttccaaaa agagccttat tttgaaggaa aagcccaagt aatcgctcaa aatggcgcat
     4981 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     5041 taaaagaaaa gggggttcaa ttgggaaaac agccaggtac gatccaccaa gttgaaaccg
     5101 agaaaaaaag tgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     5161 atcagctcat gaaagctagt gccaaagata ctttggaagc catacaaggg ttgtatgaag
     5221 ggaaatatct aagctaccca cgaacagata ccccttatat tacagaaggg gagtatgcgt
     5281 atctgttgga tcacttagac gaatacaaac actttttaaa ggccgaagcc attcctactc
     5341 ctattcatac accgaatagt cgatatgtga ataataagaa agtccaggaa cattacgcga
     5401 tcattcctac aaaaacggtc atgacggcag cggcttttga acaattaagt ccactgcagc
     5461 aggccattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aaatatacct
     5521 atgaagaaac aacgatcttg acgcaagttc agcagcttca gttaaaggca atcggaaagg
     5581 tgccgctaga tctaggttgg aagaagttat ttggaaaaga gagcgacgga aaagagaagg
     5641 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggtg gatctacaag
     5701 ttcttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcaa
     5761 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5821 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5881 atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg ctttgccaag
     5941 ccgtggaaaa agagccgctg cttacgagcg ccgaaatgac cgccaaatgg gaaagctact
     6001 tgttaaagat cggtgaacga aaaggaacac aaaccacttt tctagccaat atccaaaaat
     6061 ttgtctctca cttattggaa gtcgtaccag ggcaaatcca atccactgat tttggatcga
     6121 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     6181 gtccaaaatg ccaggaacaa gaagtcttgc tctatcaaaa ggtggcagct tgtacgtctg
     6241 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     6301 tgaaggagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6361 agggttcatt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6421 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgccg cactgcaaaa taatggaggg
     6481 ttaatcttat gcgtaatacc acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6541 ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa ttgaagaacc
     6601 agcagaggat tcaaaaagaa atcgaattag atcgataaaa tcttcctaac tattgtatac
     6661 gtataaactt atgttatact gtttttataa gtttatacgt atacaaagga gggagaaaat
     6721 gacaaaagca atggtataca agctgctaga agaatttaat ggaactttgg ctttgaagga
     6781 tgccaaaaaa gcgggcattt ctcctgtaac gatcaaacgt atggtagatc gaggggagct
     6841 ggatcgagag tatccaggtt ttttcacctt acctggtcaa tttcctgatg aattgtttat
     6901 ggcgcaaaaa aaatatgaac gcggcattat ttcacatatt accgcgttag atttgtatga
     6961 tttaacagat atgatcccta gacagatcga tctgactgta ccttacggtt atcacgtttc
     7021 tgaaaaagga ttgaaggaat tcgcagttga gctacactat acaaaagcgg aatggtatga
     7081 attaggtaag attgaaataa aaagtcgcta tgggaatcct attatagctt atgatacaga
     7141 aagaacttta tgtgacattt ggaatccctg gtataccgtt gaggatgaaa tcaaagtaaa
     7201 agcaatcaaa aactatatgg agtcggacag aaaaaatctt agaaaattga atgagtatcg
     7261 acgaatttta ccaacagaca aaacgatgcg ttcttatatt atggctttaa attaaaggag
     7321 aagatacatg acacctacgc agttaaaagc aaaagtgaag aatatttcta gagaaaaaga
     7381 agttgatccg cagcttgttt taagacattt tatgatggaa aaattcttgg aaaaaatctc
     7441 agagtctcct tatcgtgaaa attttgtgtt aaaaggcggc ttcttgattg gctcaaagta
     7501 tggcattgaa aatcgcacaa ccaaagatat tgatacgact ttacgggaaa tgaaagtaac
     7561 caaagaaaca ttaacaacgg ttctaaacga aattttttca acgccgacga aagaaggcat
     7621 tcaatttgag attcaaggaa tgaaagaaac gagagaagcc gattactatc caggatttag
     7681 cttacgtgtg ctggcacatt tggaaaacat gcggccagat tttaaggtag atgtgactac
     7741 aggtgactcg atctatcctg caacgatcac tcacagtcat aaattgatgt ttgaggatcg
     7801 aacgattcct ttggaatcct atcctacaga acaaattatt gctgaaaaat taagtgcaac
     7861 ctttgatttt ttaacagata attcgagagg aaaagatttt tatgatctct atactattcc
     7921 taaaatggag aagatcgatg agaagacctt gaaagatagt gtgagaaata catttacacg
     7981 tcgaggaaaa actgatccgt taaagatata ctatgaaaag gatatggatg tactgaaaaa
     8041 tagtcacgag ctaaaagata cctggaagaa atatcaaaaa agcaatcctt atgctgcatc
     8101 gatcacgtac gatgagacaa tggattcgat tagtaatttg atggaaaaag tgattcacgt
     8161 cgaagaaaaa gagcaaaaac tagcttatca aagacaccaa cagatgaata gagaaatgga
     8221 gagataaaga tgattaataa tgtgacatta gtaggacgct taaccaaaga tcctgattta
     8281 cgttataccg caagtggtac tgcagtagcg acttttaccc tagccgtcaa ccgaaatttc
     8341 acgaatcaaa acggcaatcg agaagccgat tttattaact gcgtgatttg gcgcaaacca
     8401 gcagaaacaa tggcgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt
     8461 caaacgcgga cttacgacaa ccagcaagga caacgtgtat atgtgactga agttgtagca
     8521 gataactttc aactattaga aaataaagca gcgacagaga gccgtgcgca cgctgatcaa
     8581 tcgtccacca gtccaagcac cgccaccttt gaacaacgga tacagcgacg cctaacaaca
     8641 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8701 atctaccgtt ttagtttgag aaagaaggag taagcatggc aaaaaaaatt agtctgacga
     8761 tggatctatt gatacccgta attgcattta tagacgggat ttctttgggc gtcactgaaa
     8821 atcctttggt aaatagaatt gcgaaaggat cactatttct tgtattcgtg tatcttgtct
     8881 gttcttatct tttaaaaaga gcaggaaagg ataattcctt ttggcgattg actaaagtca
     8941 cagtagcagc gacagctgtt atacttacgg ctattctacc gctttatatg tagagttaac
     9001 gggaaatcgt tgaacttctt gcgaaatatt gctataatga acatgaaaaa gaggagttgc
     9061 gctaacaacc cctcattaac agcaccgatt aaagaacggt ggcagaatat tagttatttg
     9121 gaaataaccg ctgacctgct aaagtagacg gttatttctt tttgtcattt ttaagcatat
     9181 ttacaatcaa actaatcaaa gcaatggtaa acataccaaa accaagaatg gtttgaattg
     9241 tctcatatgc ggacaaaagg cttactcctt ttatagattt tagcacttac attcataagc
     9301 accaccacct ttccagaagt cgccaccgtc ttcaaacttt actgtcttga ctattatagc
     9361 atatctaaaa tcgaataaaa attctcaatt ttaactgttt ttgctataat tttagcgagg
     9421 tgatcgtgat gaacaaatat gttggtttgc ttgataaaat ccgagtaata aaaacacaac
     9481 cattattggt tcgctttact ttacaaacaa ttcatgaatc gattaattgt gtagttgcag
     9541 atattgaaat tatcgataaa ttgttaatta tggatgatgg aaaatacaat attgccgtca
     9601 caggtcattt caataaacgg aatcaattag tcattgaatc gatgcaaatt agaaaccctg
     9661 atcattttac gcgatcaatg ggcatataaa taaaagaaga gctgcaatcc taatgtagga
     9721 ctgcagctct ttttgtttta catagaaaca ggaagaaagg gaaaggcctg tttaggaagg
     9781 cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc aaccgccacc gccgttcgag
     9841 aagaaaggaa gtgaacgttg cacgttcact aagataaggg gtcttgcttt gtaagacaaa
     9901 ataggaaaag cttcctctca ccaaaaggtt cgttcctgtt cgctatctct ttattgattt
     9961 gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa gggtaaatgg
    10021 cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca acaaatcaat
    10081 aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga atcaatcatg
    10141 cgaaagaaat ttgattattt gggtgttccc ttcagcgaat tattcccaaa ctatcatgca
    10201 ccacatactg tcgaatgcga ttgtggagaa agagcgaaat gtatcaaaag ctattgtttg
    10261 tatcaatgtc caacatgcgg gaaaaaatat accttgtctt atggcgatta tattttgatt
    10321 gaggagaaag gaaagaaacg ctgatgaaaa cacaacacaa attattgact gtaaaagaag
    10381 tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa gctttactta
    10441 ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact atgctatttg
    10501 aagacctagt gaagattgca ggatgtgaac tgcattatca agaaaacttg gaaacttatg
    10561 aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat atggctcaaa
    10621 attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa atggaggata
    10681 tgacgttcct atacaacgaa acacgtgttc caaaaaaaca ttataaagaa aaattgtcgg
    10741 tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tcttattgcg acgtactatc
    10801 gaacactgga agaactgaaa aagcaaaatg caaaatggtt tttccaagcc ttactatgct
    10861 ttgaaatagg tgtcaaacca agcactatta aaccttctga ataccaggct ttagaactaa
    10921 cgtatgcgaa atttatcgaa acgaaaaaag ccaagactgt ttcaagtgaa tggttagatt
    10981 attttgaaaa tatcaataag tatggcgcat attacaccat gaaaaaggaa gacaacgaaa
    11041 atgaataagg tgatttatca actaacaacg gattccatat caacgatttg aaagaaaaaa
    11101 aggtcaataa tcatttatac cgaaaagtga gaatgtgtgg aataaggaca aaaaaagcct
    11161 agtaactaag ggaatggttc ttatttcgca ccaaccgccc ccgccgctta ggtggtgcga
    11221 aataaacggg caaagcccga taacttaaga gaaagaaggt gatccatttg aaaaagatta
    11281 ttcccttatt gttagtactg tttgttggcg aaatacttat gcgagatagt gcagctgtac
    11341 agttaaacct cgctgtattt ttggtaatac tggtgttttg tttgatcttt tggcggtttg
    11401 tattagtcct cgtgagtcta ctattcaaga tgattgcagc catatttttc atggggttat
    11461 ttcgatgaaa ggagagagta ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    11521 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggcaaaagtg
    11581 aatccaggat tatgtgtatt gcgttagatt tattgattgc ctgtattgga caatatctct
    11641 attgtattcg aggaagaaag ctttacgctt gaatgcgata agttaacccg atatatcgat
    11701 ccaaaattct atgaggataa aaacctggca tgggaactgg ctttatagac agagtgctag
    11761 tgcaagtaaa ggaagaaagg aagatcacca tgaaatacaa tttattagac cataagcgaa
    11821 tgtgggtaga gaaattagag gaagacttag aacagttgga attgttaggt cataataaga
    11881 cttcaacggt gtataaagaa ggactaaaac gaacgaaaaa agcacaaaaa gaactagcaa
    11941 cactggaaag tagggttcag aaaaaagaag tagacttaac acacacttac ctttaggagt
    12001 agaaaatggc agacaagaga aaagaaaaga ttcaaaaatt actggcgctt gcggcggatg
    12061 taaatgatga ggaatcagtg agcgcattag ccaaagccca gcaattgatg atggactatg
    12121 atcttactga agatgagatt ttcaattatc atgagcagca acggatagaa gcagtagaaa
    12181 cgcacgtgat ctatcgtgga cgtccgcaaa aatggctgta tcgtttattt agaaattaag
    12241 ataaaaaagg ctagttaaca ggtttgttga ctggcctttt ttgatatgtt tgtacatgtt
    12301 ttattgtgtt ttaatttttc tcttttttat agcaagctcc ttttataata aatattccga
    12361 aaagtgaaga ccatttttta tttattaaga ctattattat tagtgagata aaatttgatt
    12421 gatcttacag aatattggtg acgattatga cactaaagat actatttatg aaaattttgc
    12481 aaaaatgttc tgaaaattac atataattgt tttataaaaa agggaggcaa ttatatgaag
    12541 aaaaaatttg ttagtatttt ttgattttag gaattgtttt attgagtgta tctactttag
    12601 gaattacagt agatgctgca acttattatg gaaatggtgt ttattgtaat actcaaaaat
    12661 gttgggtaga ttggagtaga gctcgctctg agattgttga tagaggagta aaagcttacg
    12721 ttaatggttt tacaaaagtg ctaggtggag tcggtggaag ataaaaaaga aaaacttttt
    12781 gacttaattg cgaatgcata taattcaaag tcatataata atgtagaagt aaaagaagga
    12841 tttcttcaag atgctaagaa aatacgagag gataaaccat atcctgaaat tattatatca
    12901 gtgtacaaac atttatctaa attaaaagct gaggaacg
//
LOCUS       DACIWG010000068        12728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.072, whole genome shotgun sequence.
ACCESSION   DACIWG010000068 DACIWG010000000
VERSION     DACIWG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.072"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..305
                     /locus_tag="IYF54_002263"
     CDS             6..305
                     /locus_tag="IYF54_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9832576.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IYF54_002264"
     CDS             378..1739
                     /locus_tag="IYF54_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ9832577.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IYF54_002265"
     CDS             1761..2021
                     /locus_tag="IYF54_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832578.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IYF54_002266"
     CDS             2035..2787
                     /locus_tag="IYF54_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ9832579.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IYF54_002267"
     CDS             complement(2881..3486)
                     /locus_tag="IYF54_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832580.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IYF54_002268"
     CDS             complement(3539..4513)
                     /locus_tag="IYF54_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ9832581.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IYF54_002269"
     CDS             complement(4891..5670)
                     /locus_tag="IYF54_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9832582.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYF54_002270"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYF54_002270"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ9832583.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IYF54_002271"
     CDS             7161..8558
                     /locus_tag="IYF54_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ9832584.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IYF54_002272"
     CDS             8639..9079
                     /locus_tag="IYF54_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ9832585.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IYF54_002273"
     CDS             9227..9553
                     /locus_tag="IYF54_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9832586.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IYF54_002274"
     CDS             9554..10303
                     /locus_tag="IYF54_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832587.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IYF54_002275"
     CDS             complement(10383..10838)
                     /locus_tag="IYF54_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9832588.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IYF54_002276"
     CDS             complement(11265..11540)
                     /locus_tag="IYF54_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832589.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IYF54_002277"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IYF54_002277"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ9832590.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4077 a         2187 c         2595 g         3869 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaac
//
LOCUS       DACIWG010000069        11813 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.073, whole genome shotgun sequence.
ACCESSION   DACIWG010000069 DACIWG010000000
VERSION     DACIWG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.073"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..205)
                     /locus_tag="IYF54_002278"
     CDS             complement(<1..205)
                     /locus_tag="IYF54_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832591.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="IYF54_002279"
     CDS             complement(221..835)
                     /locus_tag="IYF54_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ9832592.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="IYF54_002280"
     CDS             complement(982..1665)
                     /locus_tag="IYF54_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ9832593.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="IYF54_002281"
     CDS             complement(1774..2232)
                     /locus_tag="IYF54_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ9832594.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="IYF54_002282"
     CDS             complement(2316..4079)
                     /locus_tag="IYF54_002282"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ9832595.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="IYF54_002283"
     CDS             complement(4300..5760)
                     /locus_tag="IYF54_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832596.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="IYF54_002284"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="IYF54_002284"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ9832597.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="IYF54_002285"
     CDS             complement(6917..8344)
                     /locus_tag="IYF54_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9832598.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="IYF54_002286"
     CDS             complement(8375..9589)
                     /locus_tag="IYF54_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ9832599.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9930..10172)
                     /locus_tag="IYF54_002287"
     CDS             complement(9930..10172)
                     /locus_tag="IYF54_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ9832600.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10204..10761)
                     /locus_tag="IYF54_002288"
     CDS             complement(10204..10761)
                     /locus_tag="IYF54_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ9832601.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10774..10962)
                     /locus_tag="IYF54_002289"
     CDS             complement(10774..10962)
                     /locus_tag="IYF54_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ9832602.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="IYF54_002290"
     CDS             complement(10975..11541)
                     /gene="amaP"
                     /locus_tag="IYF54_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ9832603.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3465 a         2447 c         1934 g         3967 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca
     9841 tataaaatat ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc
     9901 atttcaacat aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact
     9961 aatactaaaa tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt
    10021 ccccaagttc ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt
    10081 gcgatgatcc ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata
    10141 ccgccgacga ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac
    10201 ttcttattgt acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc
    10261 agattttgct ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt
    10321 tactttgtct tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc
    10381 aacattcaca ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat
    10441 caattctttg atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc
    10501 tagatccaca gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc
    10561 tgtgttgact aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc
    10621 atctacatct ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa
    10681 ttcaccattc atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt
    10741 attcatgttt ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat
    10801 tcctgactgt ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa
    10861 caatgtcttg aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc
    10921 aaagataata gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc
    10981 cactcgagag tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac
    11041 tgccactttg accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg
    11101 catcaataca cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg
    11161 gatatggatt cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc
    11221 ttcgtgtaaa tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc
    11281 atcttcccgt ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa
    11341 gatgataata gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta
    11401 gtaattcgtt aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc
    11461 attgatcaac gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat
    11521 tatttttaac cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa
    11581 ctctaccgac aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg
    11641 ctaccaaaca ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa
    11701 ttgcttactt attctctatt tacttttgat tgctggctat acttaaagga tattattcaa
    11761 gattaaaaat tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DACIWG010000070        11731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.074, whole genome shotgun sequence.
ACCESSION   DACIWG010000070 DACIWG010000000
VERSION     DACIWG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.074"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            395..601
                     /locus_tag="IYF54_002291"
     CDS             395..601
                     /locus_tag="IYF54_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ9832604.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(803..1804)
                     /locus_tag="IYF54_002292"
     CDS             complement(803..1804)
                     /locus_tag="IYF54_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832605.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1809..3722)
                     /locus_tag="IYF54_002293"
     CDS             complement(1809..3722)
                     /locus_tag="IYF54_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ9832606.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3890..4396)
                     /locus_tag="IYF54_002294"
     CDS             complement(3890..4396)
                     /locus_tag="IYF54_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ9832607.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4556..4996
                     /locus_tag="IYF54_002295"
     CDS             4556..4996
                     /locus_tag="IYF54_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9832608.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5022..6179)
                     /locus_tag="IYF54_002296"
     CDS             complement(5022..6179)
                     /locus_tag="IYF54_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832609.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6182..6538)
                     /locus_tag="IYF54_002297"
     CDS             complement(6182..6538)
                     /locus_tag="IYF54_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832610.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="IYF54_002298"
     CDS             complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="IYF54_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ9832611.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8006..8845)
                     /locus_tag="IYF54_002299"
     CDS             complement(8006..8845)
                     /locus_tag="IYF54_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ9832612.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9030..9917
                     /locus_tag="IYF54_002300"
     CDS             9030..9917
                     /locus_tag="IYF54_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ9832613.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNNDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10511..11206)
                     /locus_tag="IYF54_002301"
     CDS             complement(10511..11206)
                     /locus_tag="IYF54_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ9832614.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11190..11588)
                     /locus_tag="IYF54_002302"
     CDS             complement(11190..11588)
                     /locus_tag="IYF54_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ9832615.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3501 a         2323 c         1756 g         4151 t
ORIGIN      
        1 tctaaaaaat taaaaaaaat tttggtgaat taagtatttc cttgataaca ctggcttttg
       61 gcctcatttt aggttatcat ttgtaatttt atttcacagt tgatatgatt gttatgtaaa
      121 ataaaaaagt ttaggataca tcgaattttt ggggaaattc ttttgggggt atcctaaatc
      181 ttttctatta tattcccata ttttctatta ttggtctaga cgtatgatgg taaactttgt
      241 acctatctga ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc
      301 tagatgatca ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat
      361 ttatcataca attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct
      421 ttttttcgct agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa
      481 taacaacgga atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct
      541 tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg
      601 acttataaaa tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg
      661 aataaaaggt gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac
      721 catgaaaagc ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca
      781 acctcttttg catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt
      841 cttgcgcttg tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct
      901 ccgcataatc tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt
      961 ccatttgttt cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac
     1021 gatccgcatg tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat
     1081 tatcaaagta agcccatcca catttttcat aaagaaagct agagatttta tcacttactt
     1141 gagtaagtgc agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt
     1201 tctccaaagt cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca
     1261 aatattctcc actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat
     1321 ctgcttgatc ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg
     1381 gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg
     1441 tgtctgaaca tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg
     1501 cgcctccatg catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga
     1561 tatccatatt attatttttg tactttgaag gataggatat ttcttttttt atatcgattt
     1621 gttcgatttt ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt
     1681 caaattgttt tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata
     1741 cagtgactaa aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt
     1801 tcattcattt accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa
     1861 aatccacctg aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt
     1921 ttttttgcat ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca
     1981 cttgattgat cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct
     2041 tgcttttttg gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc
     2101 tcattaaaaa tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc
     2161 ttttgctctg tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct
     2221 ttgagtagac ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact
     2281 actttcaaaa agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt
     2341 ccatgccctg ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat
     2401 ttttgcgcgt tagacacagg tactacatca tctgctgttc catggataat caatttaggc
     2461 aatgaatctg acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga
     2521 tcaaaatagt attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg
     2581 atgtctaact gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta
     2641 aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg
     2701 acaggaatat ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa
     2761 agtaaaatct tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca
     2821 tctgactcat attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa
     2881 tcaacaaata ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc
     2941 ccgttattcc aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga
     3001 tcgacataat atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga
     3061 tgaagttgtt ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct
     3121 gtacctccaa gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga
     3181 taagtagcaa tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc
     3241 tcagtgacta ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt
     3301 tctgtcatct gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta
     3361 ccgatatcat cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg
     3421 tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc
     3481 gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt
     3541 tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc
     3601 cgagattccg attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta
     3661 cttacagcaa ataagatgac caacgctccc tttaatactt gttttgttct caagctattc
     3721 actccttttt atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa
     3781 taaatggttt atcccataaa aatagaacag gattattttc tcattttacg taataaagca
     3841 gaatggataa accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca
     3901 aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt
     3961 tcataaaagg attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta
     4021 atccatgagc aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt
     4081 cgatcgaatg acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat
     4141 tcgcatgggt ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct
     4201 cccatggttt tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt
     4261 tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt
     4321 cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt
     4381 cggctgtgtt tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta
     4441 tctagctagt tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt
     4501 ttcgactatt tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat
     4561 gattaaattg tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat
     4621 cgtccatgat ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga
     4681 atttatccac ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc
     4741 tattagttat aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat
     4801 tgagattcgt acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa
     4861 acttttgact ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa
     4921 gcaagagctc aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct
     4981 ttttgcaatg tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat
     5041 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     5101 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     5161 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     5221 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     5281 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     5341 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     5401 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     5461 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     5521 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     5581 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     5641 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     5701 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     5761 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     5821 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     5881 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     5941 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     6001 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     6061 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     6121 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     6181 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     6241 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     6301 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     6361 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     6421 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     6481 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     6541 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     6601 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     6661 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     6721 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     6781 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     6841 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     6901 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     6961 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     7021 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     7081 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     7141 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     7201 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     7261 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     7321 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     7381 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     7441 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     7501 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     7561 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     7621 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     7681 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     7741 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     7801 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     7861 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     7921 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     7981 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     8041 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     8101 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     8161 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     8221 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     8281 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     8341 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     8401 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
     8461 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
     8521 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
     8581 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
     8641 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
     8701 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
     8761 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
     8821 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
     8881 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
     8941 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
     9001 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
     9061 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
     9121 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
     9181 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
     9241 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
     9301 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
     9361 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
     9421 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
     9481 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa
     9541 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
     9601 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
     9661 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
     9721 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aatgacaaat
     9781 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
     9841 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
     9901 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
     9961 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
    10021 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
    10081 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
    10141 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
    10201 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc
    10261 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    10321 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    10381 catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg
    10441 aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg
    10501 agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga
    10561 aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta
    10621 atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg
    10681 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10741 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10801 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10861 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10921 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10981 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    11041 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    11101 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    11161 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    11221 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11281 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11341 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11401 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11461 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11521 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11581 atcgtcatct ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg
    11641 tttctagatt ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt
    11701 gtgatttctc agagtacaat ggatgtatgg a
//
LOCUS       DACIWG010000071        11247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.075, whole genome shotgun sequence.
ACCESSION   DACIWG010000071 DACIWG010000000
VERSION     DACIWG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.075"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYF54_002303"
     CDS             complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYF54_002303"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ9832616.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2280..2558
                     /locus_tag="IYF54_002304"
     CDS             2280..2558
                     /locus_tag="IYF54_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832617.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2655..3476
                     /locus_tag="IYF54_002305"
     CDS             2655..3476
                     /locus_tag="IYF54_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ9832618.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3761..4771)
                     /locus_tag="IYF54_002306"
     CDS             complement(3761..4771)
                     /locus_tag="IYF54_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9832619.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4783..5736)
                     /locus_tag="IYF54_002307"
     CDS             complement(4783..5736)
                     /locus_tag="IYF54_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ9832620.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5726..6796)
                     /locus_tag="IYF54_002308"
     CDS             complement(5726..6796)
                     /locus_tag="IYF54_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832621.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6802..7845)
                     /locus_tag="IYF54_002309"
     CDS             complement(6802..7845)
                     /locus_tag="IYF54_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832622.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7846..8787)
                     /locus_tag="IYF54_002310"
     CDS             complement(7846..8787)
                     /locus_tag="IYF54_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ9832623.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8871..9218
                     /locus_tag="IYF54_002311"
     CDS             8871..9218
                     /locus_tag="IYF54_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ9832624.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9288..10949)
                     /locus_tag="IYF54_002312"
     CDS             complement(9288..10949)
                     /locus_tag="IYF54_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ9832625.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3173 a         2287 c         2046 g         3741 t
ORIGIN      
        1 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
       61 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      121 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      181 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      241 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      301 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      361 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      421 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      481 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      541 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      601 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      661 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
      721 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
      781 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
      841 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
      901 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
      961 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1021 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1081 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1141 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1201 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1261 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1321 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1381 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1441 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1501 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1561 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1621 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1681 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     1741 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     1801 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     1861 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     1921 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     1981 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2041 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2101 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2161 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2221 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2281 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2341 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2401 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2461 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2521 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2581 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2641 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     2701 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     2761 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     2821 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     2881 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     2941 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3001 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3061 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3121 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3181 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3241 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3301 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3361 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3421 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3481 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3541 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3601 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3661 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     3721 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     3781 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     3841 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     3901 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     3961 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4021 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4081 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4141 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4201 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4261 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4321 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4381 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4441 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4501 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4561 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4621 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4681 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     4741 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     4801 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     4861 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     4921 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     4981 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5041 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5101 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5161 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5221 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5281 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5341 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5401 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5461 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5521 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5581 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5641 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     5701 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     5761 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     5821 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     5881 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     5941 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6001 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6061 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6121 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6181 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6241 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6301 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6361 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6421 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6481 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6541 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6601 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6661 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     6721 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     6781 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     6841 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     6901 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     6961 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7021 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7081 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7141 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7201 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7261 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7321 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7381 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7441 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7501 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7561 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7621 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7681 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     7741 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     7801 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     7861 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     7921 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     7981 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8041 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8101 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8161 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8221 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8281 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8341 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8401 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8461 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8521 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8581 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8641 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     8701 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     8761 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     8821 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     8881 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     8941 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9001 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9061 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9121 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9181 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9241 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9301 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9361 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9421 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9481 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9541 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9601 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9661 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
     9721 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
     9781 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
     9841 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
     9901 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
     9961 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10021 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10081 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10141 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10201 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10261 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10321 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10381 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10441 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10501 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10561 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10621 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10681 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    10741 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    10801 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    10861 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    10921 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    10981 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11041 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11101 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11161 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11221 aaagtcttgt cacagtccct tatctga
//
LOCUS       DACIWG010000072        11119 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.076, whole genome shotgun sequence.
ACCESSION   DACIWG010000072 DACIWG010000000
VERSION     DACIWG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.076"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            102..338
                     /gene="secG"
                     /locus_tag="IYF54_002313"
     CDS             102..338
                     /gene="secG"
                     /locus_tag="IYF54_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ9832626.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            450..1196
                     /locus_tag="IYF54_002314"
     CDS             450..1196
                     /locus_tag="IYF54_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ9832627.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1199..3556
                     /gene="rnr"
                     /locus_tag="IYF54_002315"
     CDS             1199..3556
                     /gene="rnr"
                     /locus_tag="IYF54_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ9832628.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            3578..4042
                     /gene="smpB"
                     /locus_tag="IYF54_002316"
     CDS             3578..4042
                     /gene="smpB"
                     /locus_tag="IYF54_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ9832629.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4344..5063
                     /gene="atpB"
                     /locus_tag="IYF54_002317"
     CDS             4344..5063
                     /gene="atpB"
                     /locus_tag="IYF54_002317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ9832630.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5116..5331
                     /gene="atpE"
                     /locus_tag="IYF54_002318"
     CDS             5116..5331
                     /gene="atpE"
                     /locus_tag="IYF54_002318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ9832631.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            5422..5946
                     /gene="atpF"
                     /locus_tag="IYF54_002319"
     CDS             5422..5946
                     /gene="atpF"
                     /locus_tag="IYF54_002319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ9832632.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5933..6475
                     /locus_tag="IYF54_002320"
     CDS             5933..6475
                     /locus_tag="IYF54_002320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ9832633.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            6498..8054
                     /locus_tag="IYF54_002321"
     CDS             6498..8054
                     /locus_tag="IYF54_002321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ9832634.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            8068..8970
                     /locus_tag="IYF54_002322"
     CDS             8068..8970
                     /locus_tag="IYF54_002322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ9832635.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            8993..10399
                     /gene="atpD"
                     /locus_tag="IYF54_002323"
     CDS             8993..10399
                     /gene="atpD"
                     /locus_tag="IYF54_002323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ9832636.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10413..10835
                     /locus_tag="IYF54_002324"
     CDS             10413..10835
                     /locus_tag="IYF54_002324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ9832637.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3684 a         1982 c         2455 g         2998 t
ORIGIN      
        1 ttttgaactt tcagctgtta ttccatttat atagagttgt cacggacttt tatctatgat
       61 aaagttaaag agacgatatc ttttgggaag gaggctgcga tatgtataat ttaattttgg
      121 gaatcgtgat cgttttatcg attgttatgg tcattgcgat tatgatgcag ccaagcaagc
      181 aaaatagtgc agcgagtgca tttaccggcg gtgcggatca actattcggc aaacaaaaag
      241 ctcgcggctt tgaagctgtc atgcagcgat caactgcagt tatgggagct atctggatga
      301 tcttattatt tgtcctagca tatttatctt cgaagtagtt gaataagcct ctcgcaaaac
      361 ggcgggcggc tttttttatt gcaaagagaa cgcttaagaa gaagttatga aagaaactat
      421 ggtaaaatga ggcgaaagag gtgagacgag tgagacaatt accaaaaccc ctttatgcaa
      481 aacatggaaa gcgagcggtt ttgctgcttc acgcttactc aggaagtcca aacgatgttc
      541 gaatgctcgc acgatttttg gaaaaagcag attatacagt ttatgcgccg ctttttaaag
      601 gacacggaac aatggagcca tatgatatct tgcaagaaaa agcagaaagc tggtgggcag
      661 acactaaaaa agccatccat tttttacagt ctgagcagtt ttctgatatt gccgtattag
      721 gattatcaat gggcggcatc tttgctgtac gagcactaga agaagaatca gtgattggcg
      781 gcggtttttt ctgttcgcct ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa
      841 aatacgtacg tcaagtgctg aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag
      901 cagttgctta ccgctcgtta gctgaacaac aattaatgga tatacaagat caggcagcaa
      961 tcgtagaaag taggttgtca gatatccaac agccgatatt tttagctcaa gctgggaaag
     1021 atgaaatgat cgaccctaat ggcgtatttg aaactgcacg caagttgagc cgtcagcgag
     1081 tgacacttca atggtatcct gaaagtggtc atgtgattac agttggtaca gcaagacgcg
     1141 aattagaaaa agacgtcttg gaatttttag aaaaattacc ttggaatgag gaataaatat
     1201 gacaaaagaa acgataaaag agaaaattct ctttttcatg gaaagcagtc gcaaacgcag
     1261 tttttctatg gaagaaatcg ctgaaggttt agggttccaa aaaagcgatg attttaaatt
     1321 actagtacaa acgattgctc aaatggaaag agaacagagt atagttttca ataaaaaagg
     1381 aaaagtgaaa cttcctttaa aaccaatctt gatcgaagga acatttcgag caaatgaacg
     1441 aggatttgga tttgtaacga ttgatcccga agaagatgac atctatatcc caaaagaagc
     1501 gactggtttt gcaatggatg gagacaccgt agcaatcgat atcatgaagc gagcaaatac
     1561 agatatggat cgcggagcag aggggaaagt agtcgagatt cgagaccggg cgactactca
     1621 gttagcaggg gaattcgttg cttattcaga agaagaaata caagaaacag atctttacgg
     1681 cgttgttatc ccgaaagata gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg
     1741 agttcgtcca gttgacggaa gtattgtgct gatcgaactg acccattacc ctgaaaaagg
     1801 gtatgcgact agtttagaag ggataatcaa gcaagtgatt ggtcataaga atgaccctgg
     1861 catggatatc ctctcaattg tcgtggctaa tggtatccct acgaaatttc ctgatgatgt
     1921 attgtctgaa gcagatgaag ttccagatca aatcagcgaa aaggatataa ctggacgtag
     1981 agatttaaga aatcagctca ttgttacgat tgatggagaa gatgcaaaag atctagatga
     2041 tgcagttacg gttaaaaaat tagataacgg taattatttt cttggagtac acattgcaga
     2101 tgtttcttac tatgtgacac aaggcagtca attggacaga gaagcatatg aaagagggac
     2161 aagtgtatat ttgactgatc gagtgattcc gatgatcccg caacgtttat cgaatggtat
     2221 ctgttcactg aatccccatg ttccaaggct gaccatgagt tgcgaaatgg agattgatcc
     2281 aaatggacaa gtagtctctc atgatatttt cccaagtgtg atccaaacaa cagaacggat
     2341 gacctacaca gctgtcaatc aaatcttaga agatcaagac gaagaaacga tgcaacgcta
     2401 tgctcagctc gtacctatgt tccaagaaat gggtgaattg catcgaatcc ttgaaacgat
     2461 gcgaatcaaa cgtggcgcga tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc
     2521 tgaaggacac ccaaatgaca tccagctaag aagcagagga attggtgaac gcttgatcga
     2581 atctttcatg cttgcggcca atgaaacggt agctgaacat tataataaat taaatctgcc
     2641 gtttatttac cgaatccatg aacagccaaa agaagaaaaa atgcagcgct tctttgattt
     2701 tgcttcagct ttgggcattc tggtccgagg aacgaaagga acgatcacac caaaagattt
     2761 acagcatgtg atcgaaggag tagaagacaa accagaagct gcagttatca atactatgtt
     2821 gctaagaagc atgcaacaag cacgctattc agaagacaat tttgggcatt atggcttagc
     2881 ggcagattac tacacacact ttacatctcc tattcgacgc tatcccgatc taatcgtcca
     2941 tcgtctgatc cgttcttaca gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc
     3001 ggaacttcct gaaattgcag atcatagttc taagatggaa cgtcgtgcag tagaaacaga
     3061 acgtgaagta gattccatga agaaagcaga attcatgatg gacaaagtgg gagaagaatt
     3121 cgatgggatc atcagctctg ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt
     3181 agaaggattg atccatatca atgaattgaa acaggactac ttccattttg ttgaaaatca
     3241 tttagctctt gtaggtgaaa gaactggtct gacttttaaa atcggacaaa aagtgcgcgt
     3301 aaaagtagtc aaagctgatc cagatgaacg agcagtggat tttgaactcg tttccgcaga
     3361 agaggtcact gcgttggaac gtccaaaagc acgcaatgga aaatctggaa aacgagaagg
     3421 aaaacaagca ggcaaacgct cagaaaaaaa tggcaagaag aacaaacgat atagtgatag
     3481 taagcaaacg ttcggtaaga aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa
     3541 gaaaaagaaa aaataaaatg agttggagga aacagctatg ccaaaaggcg agggaaaact
     3601 aattgcacaa aacaagaaag ctcgccatga ttattcgatc atcgacacga tggaagcagg
     3661 gatggtcttg caaggaacgg agatcaagtc gatacgaaac agccggatca atctgaaaga
     3721 tggatttatt cgcgtccgca acggggaagc tttcttgcat aatgttcata tcagtcctta
     3781 tgaacaagga aatattttta atcatgatcc gttgcgcacg agaaagttat tattacacaa
     3841 gaaacaaatc gttcgtcttg aaaacgaatt gaaaaacact ggaatcactg ttgttccttt
     3901 aaaagtctat attcgtaacg gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa
     3961 atcgtatgat aaacgggaag atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac
     4021 attaaaaaat ttctctagat aatttcatta tcctttttcg catattcggt gcttttgctt
     4081 ttacttaatg ataaaatttg ttagcattta atctgataaa acatgattat cacaggtttt
     4141 ttcttagatc aggagcaccg tgcatgggga aaaattcgct tcgtttttga ctgaaataaa
     4201 aaagtgtcta attttcatga aaatgttttg aattttctgt ttcatggtgg tatgataccg
     4261 tgtggttaga aaccgattgg aaaaaaccag atacgcacat gaattgattt tatgtgaatt
     4321 tcggggaaaa gaggtgaaat ggattggatg aacgctcgct aacgtttcac attggaccaa
     4381 tttggtttga tggtacagtt tgtataatgg tgttactgac ctgtttgatt gttttcttct
     4441 tagcttactt ttttacaaga aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag
     4501 aatgggtcat tgattttagt cgaggaatcg taacagataa tctgcctaga aaagaattga
     4561 gcaatttcca tttgttggca tttacattgt ttttatttgt ttttgttgca aacaacatag
     4621 gtttagtaac aaaaatcgtc ttacctaacg aaacaacact ttggaaaagc ccgacagcag
     4681 acccgtttgt tactttaact ttggcattta taatgatctt acttactcac ttattcgggg
     4741 tgaaaaagtt aggcttcaaa ggatatttcg taaattcgtt cttgaaaccc tatagtttta
     4801 tgtttccgat gaaactgatt gaagaattta caaatttatt aacacttgct ttacgtcttt
     4861 atgggaatat ttatgcgggg gaagttttgc taaccttgat tgcaaaaatg atgacaagct
     4921 taggctggtt ctcattacca ttagcaattc cattagagat ggtctggatc gcgttctcct
     4981 tgtttattgg gagcatccaa gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc
     5041 ataagattga agtagaagaa taatactcgg ttaaaaaaca aactactatt tatctatttt
     5101 aggaggaaaa caattatgaa ttatatcgca gcagcaatcg caatcatggg agcagcaatc
     5161 ggagccggtt atggtaacgg tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa
     5221 cctgaaatgt ctggtcaatt ccgtacaaca atgtttatcg gggtagctct agttgaagct
     5281 gttcctatct taggggtagt tatcgccttg attctagttt ttgccgttta attgaatcag
     5341 tattaaggca gaaacaacat cgtttgttac tgctttttta catggaaact agaaatactc
     5401 caaagaaagg aaggctcgcc tatgctgaat caattggcaa ttgcagaagt tggcaatgcg
     5461 atgttaggta atatcatcgt tgtcagcggc tcatttttga tactactggc tctcttgaag
     5521 cactttgctt ggggaccaat cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat
     5581 gatttagatt ctgcagaaca atctcgcatc aactcagcga aaatggaaca agaacgcgaa
     5641 caacaattgt tagcctctcg ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt
     5701 ggagaattaa gccgccaaaa tattttgaag gatgctcaag aagaagcagc tcgtctaaaa
     5761 agcaaagccc aagctgatat cactgtagaa cgtgattcag cgctgaactc tgtaaaagac
     5821 gacgttgcag aactctctct tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca
     5881 gaaatgcatg aatcattaat caatcaatac attgaaggtc taggttcttc aaatgaaact
     5941 agataaatat acagtaggaa gacgttacgg gaaagcttta tttgaactgg cgatcgattc
     6001 aaatcaagca gaagaaatct atcaagatct cgtaagttta cgtcaaatct atgaacaagt
     6061 acctggatta gggaatatgt taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat
     6121 catggacaag cttgtcagcg gttatgatgg gatcgttaaa aactttttag aagttgttta
     6181 tgagtataac cgaatggatg accttttgtt catgatcgaa gaatatgaac gtcgatatga
     6241 tgaacatcaa gggttactat tgggaagtgt cacaacagca gtcccacttt cagacgaaca
     6301 acgaatacaa ttagaaaaga atgttgcaaa aacaatggac tatcaatcag ttgaattaaa
     6361 acaaatcgtt gattcttcta ttattggtgg cgcgattgtt gaagccaacc atcgagtaat
     6421 cgacggaagc attcgcacgc aattagaaaa aatgcgtaat caattaaaca gatagtacaa
     6481 agaggtaggt gaatcgaatg gccatcaaag cagaagaaat tagtgccttg attaaagaac
     6541 aaattgaaaa ttatcaaaac gtgctttcag tcgaagaaat cggtaccgta acttatgtag
     6601 gtgacgggat cgctcgtgca catggtttag aaaacgcgat gagtggtgag ttgcttgaat
     6661 tttcaaacgg ctcatacgga atggcgcaaa acttagagtc aaatgatgta ggtatcatta
     6721 tccttggcga tttcgagtcc attcgtgaag gagataaagt aaaacgaaca ggtaagatca
     6781 tggaagttcc agttggagag gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa
     6841 tcgacggact aggtgaaatc gttactgaca aagctcgtcc agtagaagcg atggcaccag
     6901 gtgttatgca acgtaaatct gttaacgaac caatgcaaac agggctaaaa gcgatcgacg
     6961 cccttgtgcc aatcggacgc ggacaacgtg aattagtcat cggtgaccgt aaaacaggga
     7021 aaacttctat tgcgatcgat acgatcatca accaaaaagg tcaagatatg atctgtatct
     7081 atgtagcaat cggacaaaaa gattctacag ttcgtacaca agttgaaaca ttgaaaaaat
     7141 atggcgcaat ggattacaca atcgttgtga atgccggtgc gtctcaacca gcaccattgc
     7201 tttatatcgc accatatgct ggtactgcaa tgggtgaaga attcatgtac aacggtaaac
     7261 atgtattgat catttttgat gatctttcaa aacaagctgt tgcttatcgt gaactttcct
     7321 tgctacttcg ccgtccacca ggtcgtgaag cttatcctgg ggatgttttc tacttgcact
     7381 cacgtttatt agaacgtgca gcaaaattaa gtgatgattt aggtggcggt tcaatgactg
     7441 ccttaccatt tgttgaaaca caagctggag atatctctgc ttatatccca acaaacgtta
     7501 tttcgatcac tgatggacaa atcttcttag aaagcgactt gttctacgca ggtactcgtc
     7561 cagcggttga tgccggacta tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa
     7621 tgaaaaaagt tgctgggact ttacgcctag acttagcaag ttaccgtgaa ttagaagcat
     7681 ttacacaatt cggttctgac ttggatgcag caacacaagc aaaactaaat cgtggacgcc
     7741 gtacagtaga aatcttgaaa caaaaattgc atgcaccgct agctgttgaa aaacaagtag
     7801 tgattttgta tgctttgaca catggattct tagatagtat ccctgtagac agtattttag
     7861 acttcgaaca tgaattattc gaatatctgg ataccaatca ttcagatatc tttgaaacaa
     7921 tccgtacaac aaaagatctt ccagaagaag aagcgttgaa cagtgcgatt caggaatata
     7981 aagacatgtt cttagctaca aaaggaagta attcctctac agaagataaa ttgaaatcca
     8041 ttcaaaatgc atagtgaggt gagatgaatg ggtgcttcat taaatgaaat caagacacgt
     8101 atcgcttcaa cgaaaaaaac gagccaaatc acgcgggcaa tgcagatggt gtctgcttct
     8161 aagttgacaa aatctgaagc ttcttcacag aagtttcagg tttatgcaac aaaagtaaga
     8221 gaaatcgtta cgcatttgac tgctactcag ttaaatgata ttgcctcaga aaatcctaga
     8281 ggggatatca attacaacag catgctgctc agccgtgctg tgaagaaaac tggttatatc
     8341 gtcatcactg cagatggcgg tttagtcggt ggatataaca gctcgatatt gaaacaaacg
     8401 atgtctattt tagaagaaga ccaccaatca cccgatgatt acgtgatgat cgcaattggc
     8461 ggaactggtg cagacttctt taaagcacgc ggtatcaatc tagcatatga gctgcgcaat
     8521 ctatctgatc agccaagctt tgatgaagtc cgcaaaatcg taaatatggc aacaactatg
     8581 ttccaaaacg aagtattcga tgaattatac gtatgttata accatcatat caattcgcta
     8641 actagtcagt ttcgtgtaga aaaaatgctg ccaatctcag acttagatcc agaagaagca
     8701 acgactttcg aacaagaata tatttttgaa ccttcaaaag aagaaatctt gtctcagtta
     8761 ttgcctcagt atgctgaaag tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa
     8821 catgctgctg gtatgacagc aatgaaaaca gcaactgata atgctgctaa tatcattgat
     8881 gagctgacgg tttcttataa ccgtgcaaga caaggggcta ttacccaaga aattaccgaa
     8941 attgttgccg gagcttcggc actagaatag tattgagatt ggaggaaaaa agatgagttc
     9001 aggcaagatt gttcaagtaa tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc
     9061 cttaccagat atcaacaacg cattagttgt ttataaaaat gacgaaaata aatcaaaagt
     9121 tgttcttgaa gcagcgttag aattagggga cggagtgatc cggaccattg cgatggaatc
     9181 aacagatggt ttacaaagag gaatggaagt cattgataca ggcaaagcaa tctctgttcc
     9241 tgtaggtaaa gaaacattag gtcgcgtatt caacgtacta ggagatacga tcgatttaga
     9301 aacacctttc ccagaagatg cggaaagaag cgaaattcat aaaaaagcac cagcctttga
     9361 cgaattaagt acaagtacag aaattttgga aacagggatc aaagttatcg atttgcttgc
     9421 cccatattta aaaggtggga aagtcggact attcggtggt gccggtgttg gtaaaaccgt
     9481 actgatccaa gaactgatcc ataatatcgc ccaagaacat ggtggtattt ctgtatttac
     9541 cggtgtaggt gaacgtactc gtgaaggtaa tgacttgtat tatgaaatga aagattcagg
     9601 agttatcgaa aaaacagcca tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat
     9661 gcgtgttgct ttgactggtt tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga
     9721 tgtactgttg tttatcgaca acattttccg tttcactcaa gctggatctg aagtatcagc
     9781 cttgttagga cggatgcctt ctgcggttgg ttatcaacca acattggcaa cagaaatggg
     9841 tcaattacaa gaacgtatca catctacgaa aaaaggttcg atcacatcga tccaagcaat
     9901 ctatgtgcca gccgatgact acactgaccc ggcgccggca acagcattcg cccatttgga
     9961 tgcgacaacc aacttggaac gtaaattaac agaacaaggg atttatccag cggtagatcc
    10021 attggcttct tcttcaagtg ctttagcacc tgaaatcgtg ggggaagaac attataaagt
    10081 agcaacagaa gtccaacacg tattgcaacg atatcgtgaa ttacaagata tcattgctat
    10141 cttaggtatg gatgaattat ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt
    10201 ccaattcttc ttgtcacaaa actttaacgt ggctgaacaa tttactggtc ttccaggttc
    10261 atatgtccct gtagaagaaa cagtgaaagg attccgcgaa atcttggaag gcaaatacga
    10321 tgatcttcct gaagaagctt tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc
    10381 gaaaacacta ggctactaga aaggggtgcc ccatggatca atatttaaca gtaaatgtgg
    10441 taactcctgc cggtttggtc tatgatcacc atgcagctat cgtcgttgca cgtacgacag
    10501 ctggtgagct tgggattttg cctaaacatg caccaatcat cgttccattg acgattgatg
    10561 aagttcgtgt aaaacgaaca gattcagata cccacgtcga ttggatcgct gttaatggcg
    10621 gaatcatgga agttcgtgat aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac
    10681 gtgatatcga tgtatctcgt gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag
    10741 aagcaaaagc aaaacaagat acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag
    10801 caatcaatcg tatcaaagta tctaagcact cataaaatga gatgcggatg tttatccgtg
    10861 tttaagaaca gtaaaaagag gttatgacag aagtggtcag cttcaagaca taagaaggaa
    10921 tttacgaaaa ttgcttctca aatttttgtg aaatctcagc ttatttttcg aagaagctac
    10981 ttctggaaca ccgtatatca gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa
    11041 acaaatggaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagat
    11101 gattgaacac cgtttattt
//
LOCUS       DACIWG010000073         9967 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.077, whole genome shotgun sequence.
ACCESSION   DACIWG010000073 DACIWG010000000
VERSION     DACIWG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.077"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            8..81
                     /locus_tag="IYF54_002325"
     tRNA            8..81
                     /locus_tag="IYF54_002325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IYF54_002326"
     tRNA            101..173
                     /locus_tag="IYF54_002326"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IYF54_002327"
     tRNA            201..274
                     /locus_tag="IYF54_002327"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IYF54_002328"
     tRNA            288..361
                     /locus_tag="IYF54_002328"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IYF54_002329"
     tRNA            385..474
                     /locus_tag="IYF54_002329"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IYF54_002330"
     tRNA            487..560
                     /locus_tag="IYF54_002330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IYF54_002331"
     tRNA            579..654
                     /locus_tag="IYF54_002331"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IYF54_002332"
     tRNA            656..726
                     /locus_tag="IYF54_002332"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IYF54_002333"
     tRNA            740..813
                     /locus_tag="IYF54_002333"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IYF54_002334"
     tRNA            838..925
                     /locus_tag="IYF54_002334"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IYF54_002335"
     tRNA            947..1018
                     /locus_tag="IYF54_002335"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IYF54_002336"
     tRNA            1041..1130
                     /locus_tag="IYF54_002336"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IYF54_002337"
     tRNA            1143..1216
                     /locus_tag="IYF54_002337"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IYF54_002338"
     tRNA            1235..1307
                     /locus_tag="IYF54_002338"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IYF54_002339"
     tRNA            1311..1383
                     /locus_tag="IYF54_002339"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IYF54_002340"
     tRNA            1388..1458
                     /locus_tag="IYF54_002340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IYF54_002341"
     tRNA            1471..1544
                     /locus_tag="IYF54_002341"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IYF54_002342"
     tRNA            1556..1627
                     /locus_tag="IYF54_002342"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IYF54_002343"
     CDS             1893..3086
                     /locus_tag="IYF54_002343"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ9832638.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IYF54_002344"
     CDS             3216..4688
                     /locus_tag="IYF54_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9832639.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IYF54_002345"
     CDS             4771..5559
                     /locus_tag="IYF54_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ9832640.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IYF54_002346"
     CDS             complement(5626..6294)
                     /locus_tag="IYF54_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ9832641.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IYF54_002347"
     CDS             6409..8121
                     /locus_tag="IYF54_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832642.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IYF54_002348"
     CDS             8124..9896
                     /locus_tag="IYF54_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832643.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2902 a         1781 c         2279 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggcagaa
//
LOCUS       DACIWG010000074         9955 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.078, whole genome shotgun sequence.
ACCESSION   DACIWG010000074 DACIWG010000000
VERSION     DACIWG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.078"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(1..657)
                     /locus_tag="IYF54_002349"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IYF54_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IYF54_002350"
     CDS             complement(662..1429)
                     /locus_tag="IYF54_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ9832644.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="IYF54_002351"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="IYF54_002351"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ9832645.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="IYF54_002352"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="IYF54_002352"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ9832646.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="IYF54_002353"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="IYF54_002353"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ9832647.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="IYF54_002354"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="IYF54_002354"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9832648.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="IYF54_002355"
     CDS             4828..5145
                     /locus_tag="IYF54_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ9832649.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="IYF54_002356"
     CDS             5145..6851
                     /locus_tag="IYF54_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9832650.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVSIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="IYF54_002357"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="IYF54_002357"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ9832651.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="IYF54_002358"
     CDS             8422..8694
                     /locus_tag="IYF54_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ9832652.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="IYF54_002359"
     CDS             8715..9614
                     /locus_tag="IYF54_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ9832653.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1679 c         1995 g         3143 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tcgatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DACIWG010000075         9863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.079, whole genome shotgun sequence.
ACCESSION   DACIWG010000075 DACIWG010000000
VERSION     DACIWG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.079"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(251..1099)
                     /locus_tag="IYF54_002360"
     CDS             complement(251..1099)
                     /locus_tag="IYF54_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ9832654.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1262..2164
                     /gene="gnd"
                     /locus_tag="IYF54_002361"
     CDS             1262..2164
                     /gene="gnd"
                     /locus_tag="IYF54_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ9832655.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2166..3167
                     /locus_tag="IYF54_002362"
     CDS             2166..3167
                     /locus_tag="IYF54_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9832656.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3164..3448
                     /locus_tag="IYF54_002363"
     CDS             3164..3448
                     /locus_tag="IYF54_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9832657.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3481..4278
                     /locus_tag="IYF54_002364"
     CDS             3481..4278
                     /locus_tag="IYF54_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9832658.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4292..5125
                     /locus_tag="IYF54_002365"
     CDS             4292..5125
                     /locus_tag="IYF54_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9832659.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5143..6000
                     /locus_tag="IYF54_002366"
     CDS             5143..6000
                     /locus_tag="IYF54_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9832660.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6022..6531
                     /locus_tag="IYF54_002367"
     CDS             6022..6531
                     /locus_tag="IYF54_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9832661.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6568..6969
                     /locus_tag="IYF54_002368"
     CDS             6568..6969
                     /locus_tag="IYF54_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832662.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7081..7434
                     /locus_tag="IYF54_002369"
     CDS             7081..7434
                     /locus_tag="IYF54_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832663.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7468..7911
                     /locus_tag="IYF54_002370"
     CDS             7468..7911
                     /locus_tag="IYF54_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ9832664.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7928..9013
                     /gene="uxuA"
                     /locus_tag="IYF54_002371"
     CDS             7928..9013
                     /gene="uxuA"
                     /locus_tag="IYF54_002371"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ9832665.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9006..9719
                     /locus_tag="IYF54_002372"
     CDS             9006..9719
                     /locus_tag="IYF54_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ9832666.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3292 a         1558 c         2171 g         2842 t
ORIGIN      
        1 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
       61 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
      121 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
      181 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
      241 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
      301 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
      361 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
      421 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
      481 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
      541 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
      601 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
      661 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
      721 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
      781 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
      841 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
      901 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
      961 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
     1021 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
     1081 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
     1141 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
     1201 taaaatctat ttacaaaaaa attttttgta taatgagggt ataagaaatg gaggcgctat
     1261 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
     1321 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
     1381 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
     1441 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
     1501 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
     1561 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
     1621 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
     1681 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
     1741 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
     1801 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
     1861 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
     1921 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
     1981 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
     2041 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
     2101 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
     2161 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     2221 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     2281 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     2341 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     2401 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     2461 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
     2521 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     2581 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     2641 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     2701 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     2761 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     2821 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     2881 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     2941 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     3001 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     3061 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     3121 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     3181 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     3241 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     3301 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     3361 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     3421 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     3481 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     3541 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     3601 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     3661 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     3721 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     3781 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     3841 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     3901 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
     3961 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     4021 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     4081 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     4141 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     4201 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     4261 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     4321 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     4381 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     4441 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     4501 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     4561 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     4621 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     4681 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     4741 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     4801 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     4861 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     4921 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     4981 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     5041 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     5101 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     5161 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     5221 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     5281 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     5341 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     5401 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     5461 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     5521 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     5581 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     5641 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     5701 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     5761 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     5821 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     5881 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     5941 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     6001 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     6061 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     6121 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     6181 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     6241 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     6301 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     6361 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     6421 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     6481 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     6541 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     6601 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     6661 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     6721 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     6781 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     6841 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     6901 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     6961 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     7021 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     7081 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     7141 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     7201 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     7261 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     7321 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     7381 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     7441 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     7501 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     7561 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     7621 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     7681 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     7741 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     7801 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     7861 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     7921 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     7981 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     8041 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     8101 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     8161 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     8221 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     8281 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     8341 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     8401 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     8461 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     8521 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     8581 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     8641 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     8701 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     8761 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     8821 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     8881 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     8941 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     9001 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     9061 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     9121 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     9181 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     9241 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     9301 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     9361 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     9421 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     9481 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     9541 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     9601 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     9661 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     9721 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     9781 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     9841 aaacacaaaa cgcatctact gtt
//
LOCUS       DACIWG010000076         9818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.080, whole genome shotgun sequence.
ACCESSION   DACIWG010000076 DACIWG010000000
VERSION     DACIWG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.080"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            54..506
                     /locus_tag="IYF54_002373"
     CDS             54..506
                     /locus_tag="IYF54_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832667.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IYF54_002374"
     CDS             520..1209
                     /locus_tag="IYF54_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832668.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IYF54_002375"
     CDS             1334..3685
                     /locus_tag="IYF54_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ9832669.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IYF54_002376"
     CDS             3765..4160
                     /locus_tag="IYF54_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832670.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IYF54_002377"
     CDS             4485..5102
                     /locus_tag="IYF54_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832671.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IYF54_002378"
     CDS             5143..5442
                     /locus_tag="IYF54_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832672.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IYF54_002379"
     CDS             5476..5643
                     /locus_tag="IYF54_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ9832673.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6293)
                     /locus_tag="IYF54_002380"
     CDS             complement(5688..6293)
                     /locus_tag="IYF54_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832674.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6418..9405
                     /locus_tag="IYF54_002381"
     CDS             6418..9405
                     /locus_tag="IYF54_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ9832675.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9554..9763
                     /locus_tag="IYF54_002382"
                     /pseudo
     CDS             9554..9763
                     /locus_tag="IYF54_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3359 a         1479 c         1879 g         3101 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat tgccaattgt
     6241 cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc cataaaaaac
     6301 tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta ctgctatcat
     6361 agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag ggtttaaatg
     6421 gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc tagtacagaa
     6481 caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga gactatacga
     6541 cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt tttaggaact
     6601 tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc aactcaacta
     6661 aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta tcagcacaca
     6721 aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat agaaacatac
     6781 ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga tatgttgttt
     6841 gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat tactactttt
     6901 tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg acaattgtct
     6961 ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt aattggaact
     7021 ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc tgacgatagt
     7081 cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt ctctactgaa
     7141 aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa ttatgcattt
     7201 aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc gttgttagtg
     7261 gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc actatctaat
     7321 ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg tcttcgtacc
     7381 ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt agtaatgtcc
     7441 agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa atattctgag
     7501 gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga tcgtgaacca
     7561 atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat tccggatatt
     7621 ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga actctggacc
     7681 ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc tattaaacat
     7741 actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac taataaatgt
     7801 gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga tatttatatt
     7861 cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa aacttgggtt
     7921 caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa tgaaatgtct
     7981 ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg ttggcctact
     8041 tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact taaaaagaca
     8101 tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact tcctaaaatt
     8161 gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca atcatttaac
     8221 cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt agcagttctt
     8281 ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt tgattcttta
     8341 aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac actttcagga
     8401 gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat atggggtgat
     8461 gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc cctatattct
     8521 agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa ttttgtctct
     8581 gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga ttcgctttac
     8641 cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat catgactgat
     8701 acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt tcaatttagt
     8761 ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa cgctcactac
     8821 ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta tacccattgg
     8881 gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc aacagaatta
     8941 acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat aactgagtat
     9001 ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat atatgcacgg
     9061 caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg taatattttt
     9121 tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga gcagctcaat
     9181 gcattaggat tagttactaa tgccatcata tattggaact cactgtattt agaaaaagta
     9241 tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg caggcaatct
     9301 ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata cgaagctgaa
     9361 ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg ttcgggtttt
     9421 gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat agaaaaagag
     9481 aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg tgctaccgca
     9541 ctatttcgtt tctatgttcc atgtacccct ttatcaagaa gcgcaaacac gccaaattgc
     9601 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     9661 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     9721 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     9781 ttactaatag tctttcctaa cattgattta atagtctt
//
LOCUS       DACIWG010000077         9773 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.081, whole genome shotgun sequence.
ACCESSION   DACIWG010000077 DACIWG010000000
VERSION     DACIWG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.081"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1329)
                     /locus_tag="IYF54_002383"
     CDS             complement(<1..1329)
                     /locus_tag="IYF54_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9832676.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIKPVVLVNFNILKNISKFL"
     gene            complement(1470..2072)
                     /locus_tag="IYF54_002384"
     CDS             complement(1470..2072)
                     /locus_tag="IYF54_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ9832677.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2317..2523
                     /locus_tag="IYF54_002385"
     CDS             2317..2523
                     /locus_tag="IYF54_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832678.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2835..3554
                     /locus_tag="IYF54_002386"
     CDS             2835..3554
                     /locus_tag="IYF54_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ9832679.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3635..4318)
                     /locus_tag="IYF54_002387"
     CDS             complement(3635..4318)
                     /locus_tag="IYF54_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ9832680.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4536..6722
                     /locus_tag="IYF54_002388"
     CDS             4536..6722
                     /locus_tag="IYF54_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9832681.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7068..7478
                     /locus_tag="IYF54_002389"
     CDS             7068..7478
                     /locus_tag="IYF54_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ9832682.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7492..7848
                     /locus_tag="IYF54_002390"
     CDS             7492..7848
                     /locus_tag="IYF54_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ9832683.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(8458..8601)
                     /locus_tag="IYF54_002391"
     CDS             complement(8458..8601)
                     /locus_tag="IYF54_002391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832684.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            8521..>9773
                     /locus_tag="IYF54_002392"
     rRNA            8521..>9773
                     /locus_tag="IYF54_002392"
                     /product="16S ribosomal RNA"
BASE COUNT         3450 a         1676 c         1977 g         2670 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttttatttg
       61 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
      121 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
      181 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
      241 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
      301 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
      361 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
      421 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
      481 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
      541 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
      601 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
      661 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
      721 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
      781 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
      841 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
      901 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
      961 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
     1021 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
     1081 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
     1141 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
     1201 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
     1261 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
     1321 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
     1381 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
     1441 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
     1501 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
     1561 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
     1621 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
     1681 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
     1741 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
     1801 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
     1861 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
     1921 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
     1981 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
     2041 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
     2101 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
     2161 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
     2221 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
     2281 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
     2341 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
     2401 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
     2461 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
     2521 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
     2581 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
     2641 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
     2701 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
     2761 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
     2821 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
     2881 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
     2941 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
     3001 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
     3061 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
     3121 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
     3181 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
     3241 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
     3301 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
     3361 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
     3421 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
     3481 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
     3541 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
     3601 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
     3661 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
     3721 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
     3781 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
     3841 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
     3901 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
     3961 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
     4021 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
     4081 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
     4141 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
     4201 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
     4261 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
     4321 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
     4381 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
     4441 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
     4501 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
     4561 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
     4621 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
     4681 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
     4741 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
     4801 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
     4861 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
     4921 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
     4981 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
     5041 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
     5101 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
     5161 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
     5221 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
     5281 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
     5341 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
     5401 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
     5461 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
     5521 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
     5581 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
     5641 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
     5701 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
     5761 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
     5821 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
     5881 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
     5941 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
     6001 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
     6061 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
     6121 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
     6181 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
     6241 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
     6301 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
     6361 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
     6421 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
     6481 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
     6541 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
     6601 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
     6661 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
     6721 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
     6781 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
     6841 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
     6901 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
     6961 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
     7021 aaagaaatta ctgtagaatt ggattataat taaaaaaggg tgagaaaatg ataaaagtat
     7081 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
     7141 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
     7201 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
     7261 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
     7321 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
     7381 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
     7441 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
     7501 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
     7561 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
     7621 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
     7681 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
     7741 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
     7801 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
     7861 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
     7921 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
     7981 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     8041 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     8101 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     8161 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     8221 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     8281 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     8341 gcagccgaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     8401 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttta
     8461 acaagcaagc aatatgctag caaacaaatt gagcttaaca atcgcaagat tgttcgaact
     8521 ttttatgaga gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa
     8581 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaggag tggcgaacgg
     8641 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta
     8701 ataccgtata acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct
     8761 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga
     8821 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc
     8881 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg
     8941 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag
     9001 agtaactgtt catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc
     9061 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc
     9121 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa
     9181 ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta
     9241 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc
     9301 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga
     9361 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg
     9421 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg
     9481 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt
     9541 tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg
     9601 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt
     9661 tagttgccat cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaagg
     9721 tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt gct
//
LOCUS       DACIWG010000078         8893 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.082, whole genome shotgun sequence.
ACCESSION   DACIWG010000078 DACIWG010000000
VERSION     DACIWG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.082"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(230..1186)
                     /locus_tag="IYF54_002393"
     CDS             complement(230..1186)
                     /locus_tag="IYF54_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ9832685.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1290..1892
                     /locus_tag="IYF54_002394"
     CDS             1290..1892
                     /locus_tag="IYF54_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ9832686.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1980..3479)
                     /locus_tag="IYF54_002395"
     CDS             complement(1980..3479)
                     /locus_tag="IYF54_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ9832687.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3495..4019)
                     /locus_tag="IYF54_002396"
     CDS             complement(3495..4019)
                     /locus_tag="IYF54_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832688.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4317..5066
                     /locus_tag="IYF54_002397"
     CDS             4317..5066
                     /locus_tag="IYF54_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ9832689.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5057..5842
                     /locus_tag="IYF54_002398"
     CDS             5057..5842
                     /locus_tag="IYF54_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ9832690.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5811..6527
                     /locus_tag="IYF54_002399"
     CDS             5811..6527
                     /locus_tag="IYF54_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ9832691.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6629..7648)
                     /locus_tag="IYF54_002400"
     CDS             complement(6629..7648)
                     /locus_tag="IYF54_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ9832692.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7667..8725)
                     /locus_tag="IYF54_002401"
     CDS             complement(7667..8725)
                     /locus_tag="IYF54_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ9832693.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2677 a         1795 c         1593 g         2828 t
ORIGIN      
        1 tcgtttggct ggacttatta ccgcagagga aagcttttga acaccgttta agaaagaggt
       61 tgtgagccta ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg
      121 tttgttgaat attgtgactt gtgaccgagg agttgacagg agaacaccgt ttaaagaaga
      181 ggttgtgaaa tcagcttttt gacctgaata ttagctgaat tcacaacctc tatttttgga
      241 ttttttgttg ggagaattct gccttgattt caataaaact tttaaaagac tgcaaaaact
      301 ggaacaattc tgctcgatcc tcgaattcag ggcgactagt cggcagaggc atttgccgat
      361 acaatacata aacttcttca atacgtgtta aaatttgtct gccatcattt gactctgaaa
      421 atgtttctgc agtataaatc aataaaccat agatatgttt tccataataa gctggttgct
      481 ggattctctc tagattttcc agcatgtctt taatcacatt ggcctgtgct cttctcatag
      541 agaagtaagt ttcgtagtat agcggctgtc tgagccagta gttttcttga tgttctctgg
      601 catcttcttg gctttcgcga atgaatgtca gtaaatgttc gcatttgatc tgcacatctt
      661 ctttattttc tgataaaaga aattctgcca tttgtctcaa aatatttcga aattcttttt
      721 cgatctgtat ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa acatttgcaa
      781 gcaaagcaaa tactaatcca atcgccatta atcccgcttc attgccgatc agctgccaag
      841 acatgttttc ctccactaaa taatgagtga ccaaaaccga atttactact atcccttcgg
      901 ttaatttgaa gcgagaggca agcgggatga acaggaataa aaaaagactg aagccaaaaa
      961 tagtataccc aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat ataaaggctg
     1021 cgattcgatt catgcccgta cgcaatgtag agcgcttggt atttcctaca cttaataatg
     1081 caatgatccc tgcagctggc caatacaaca gcgataagct acccgctgtg accatcgaaa
     1141 agaagacact gatcactgtt ttgactgttc gcattcctat acgcatttac ttcccccttt
     1201 tttattttaa ttatacttga ataatgaaaa actagctatc agacaaaagt ttaggtatga
     1261 tagtaaaaag accaaaaagg agaatctcta tgcaatttgg ccgttttcga ctaggtatgc
     1321 gtacgatcaa aagtgcctta gctgtttttt tatgtatctt gttttttcat ttgactcaac
     1381 gaggaatccc gatgattgct gcattatctg ctgttttttc tttgcgtcag gacttgacta
     1441 caaccgtttc ttttggaaag tctaggatca tcggcaatac attaggtggt acattagcta
     1501 tcatttattt ttttgtcaaa agctattttc acaatgattt tttggtagaa ttgatcttgc
     1561 ttcctattct agttattatt gtgatcgtcg tgtcagatgg tattaacaat aattccggta
     1621 tcatttccgc aatagctacg ttgttgctga ttgcgttgag catccctcaa ggcgaatccg
     1681 ctttgtatgc tatccaacgt gtactagata cttttatagg gacattcatt gcaatcggta
     1741 tcaatttctt tcttcgtcct cctgaaacag aaaagaagga agaactatca gaagatttgg
     1801 tcgaactcca aaagaaagaa cgattgctaa gaacaaattt acaagagata gaagctaaga
     1861 tccaaaaaca aaagaaaaat agcgaggact aattttcttt tgcaataaaa taaaaaaatg
     1921 gagagaagaa ctttaaaaag tcttcctcca tttttgattg aacggtgaag tgtactactt
     1981 tattcttcct tcatcgagtt ttgttctgcc tcttttggcg cttcatacat atttacttgg
     2041 ctagtagtcc cacgtgtcga caaattctct acgatttcct tcaagtcaag tcctgttgtc
     2101 gcttttagtg tttcttgagt agaagaaagc aaatttgtgg cataatttgt cactcggttc
     2161 gctccactgg tttctcctga actgcttcct gtatcgacaa cagagatttt ttcgatattt
     2221 cctagcggct gtgctgcttc gcgcatcaat tgcggtagca tatcgataac catactcaag
     2281 acagctgctt cgccatattc cttgaaggca ttggcgatct tttgttttgc ttcggcttct
     2341 gcttttcctt ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg tgtcttattg
     2401 gcttccgctt ctgccagtgc ttccacacgg aatcgttctg cttcggcttc agtcacttct
     2461 ttgacttttt gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc tgcttttttc
     2521 ttcacttccg aatcatattg cttttcacga cgtgtgattt ctttttcttc cagttcgatt
     2581 tgtttttgac gttctacgat tttaatctgc atttcctgtt cgattacttg ttgttgtgct
     2641 cgggcacttt ctaaattgta ggcttggtct gcttttgctt tagcaacatc ttgttcttgt
     2701 ttgtaggttg ctaatttcag ttctttttct tttaatgatt ctgcgatctc tgtttgtcgc
     2761 tgtaattcag ctgcttgtga ttctttttct gcttgagctt ttttgatacg cgtttctttt
     2821 agagcttccg cttctgctat atctgcatct cttttgactt gggcgattct tggtttccct
     2881 aaagagtcaa gataaccgtt tttgtctctg acctctttga tagtgaaaga aacgatgatc
     2941 aaccccattt ttgcaaggtc tacagaagcg acttcttgta cgctttgact gaacttatcg
     3001 cgattttgat agatttcttc tacagtcata gaaccaagaa tcgagcgcaa gtgtccttct
     3061 aatacttctc ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa ctgttctgct
     3121 gctgtagcaa tctcttctac agatgaaccg attttgatga tcgaagtacc atcacacatc
     3181 accggtacgc cttgttccgt atacacttca ggggtggaga cgtccaattt actcgataat
     3241 agactgatcc gattcgaacg ttgaaaaact ggcaagacga atgcgccacc tccacgcaca
     3301 attttgattt tgttattgct ttcatcaatg tgcacatttt tgcttcctaa ataacttcca
     3361 ctgatgatca acgcttcatc cggttttgct gtctggtact tcgctacaaa gacgatcagc
     3421 aacatcagta aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg tatttccatt
     3481 taacacacgc tcctttaagc ttttatcatg ctttcataag gcatgacata acagattctt
     3541 tccttgatct caatgatcaa tactttcgtt ccccgctcag ctgtctgttc ttgggggctg
     3601 taaaaacaag ctggacgact aatggaaccg gtaacacttt tcaactgaat ttctcccata
     3661 cctcgtacag ggatcggtgt gatcacaaca gctacttgtc cttccagagt tttttcagaa
     3721 gacgacatcg ttgattcggc atgtttcatc ggcatcaata tatagaaatt aagcaaaaat
     3781 accaacacta tcgatatgcc tccactagca ataaataaaa ataaagatga ttgatctgtt
     3841 aaacgctcac ctagaaaacc aaataagctt gtgaaagtta accaagggat aataagcatc
     3901 ggatcaagcg ggccatcaaa atcgaataca tctccaaaaa taatcaatag gactgccaat
     3961 cctgcacaaa tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc ccctatcata
     4021 tacacgcctc cttccatttc tacgtctatc ataccaatgt ttgaaaattc tgtatagctt
     4081 cttttaccga ctcagtggtg ttttttatag gaaggttgtg tttatcgttg acaatttgtt
     4141 aaatttttgt tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc
     4201 ctttttttaa gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct
     4261 ccacattcgt ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga
     4321 accgatctag tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac
     4381 tcatccaaaa aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg
     4441 aaatcatgat tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat
     4501 attctcacgc agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa
     4561 aagtgattgg aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc
     4621 gctatttcgt ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa
     4681 caaacaacac gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga
     4741 ttattggact tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag
     4801 tactgcttgg acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg
     4861 aaaatgaagt agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca
     4921 atacacctgc aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa
     4981 aaaaatcagt gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac
     5041 cgttcggact caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct
     5101 ttttagcaat gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat
     5161 gaaattaatg gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac
     5221 ccatgaattc atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga
     5281 agaagtcacc cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt
     5341 ctcaagtctg atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc
     5401 caaagaacaa tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat
     5461 ggatgactcc atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga
     5521 agccgtgcta caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac
     5581 agaagctgaa gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat
     5641 ctcagaaaaa atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt
     5701 gaatctcatc cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac
     5761 aaatggcggt acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa
     5821 atgtcctgat aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa
     5881 gcatccctaa tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt
     5941 tcagacaaaa aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta
     6001 tccatttttt atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg
     6061 ctgcagatga tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga
     6121 tcaaaccgtt cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc
     6181 agacaatcca aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca
     6241 ccactcccat tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga
     6301 ctttaaaaaa aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac
     6361 tggctcaaaa aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag
     6421 aacaagatat attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt
     6481 atgaattgac aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat
     6541 cccatacaaa atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa
     6601 cattttattg acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata
     6661 cctcattttt cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa
     6721 tcatatttga taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag
     6781 ggtacatttc tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg
     6841 atcggaaaaa aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat
     6901 tgcttttcaa agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa
     6961 tcaagatgct accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt
     7021 cacctgggat atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa
     7081 gcgagtggaa cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca
     7141 ctttggctcc tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta
     7201 tgatccctac tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat
     7261 ctttgaaaga tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag
     7321 tcaaagataa ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac
     7381 caaaatcagc tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat
     7441 aacgaatccc atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc
     7501 tcaacaatac agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt
     7561 cataaagact gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg
     7621 gacgaacacc gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac
     7681 ctatgccatg aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa
     7741 tagttgacag taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat
     7801 aggctgctgc cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt
     7861 caaaattgac tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg
     7921 caggtacaaa taccactttg actagactgt aataccatga agttttttca gatgcagctt
     7981 ttttgaagct tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta
     8041 aaccagctaa ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa
     8101 acgctacttg gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt
     8161 ggaacagcca aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg
     8221 ctttgatatt tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca
     8281 aaaagattcg gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa
     8341 tcgcactgac gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca
     8401 acgttgtttg cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa
     8461 taatataaat aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat
     8521 tgatatcttg catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt
     8581 tcgatagaat ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta
     8641 ataatataat aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg
     8701 atcgaaaaat gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat
     8761 aaaaaaagct tttattttgc taatacgtat taacattttt aattttatct ttacattgtt
     8821 acttattgaa caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttttact
     8881 agaacacaaa aaa
//
LOCUS       DACIWG010000079         8735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.083, whole genome shotgun sequence.
ACCESSION   DACIWG010000079 DACIWG010000000
VERSION     DACIWG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.083"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(474..1061)
                     /locus_tag="IYF54_002402"
     CDS             complement(474..1061)
                     /locus_tag="IYF54_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832694.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1236..2558
                     /locus_tag="IYF54_002403"
     CDS             1236..2558
                     /locus_tag="IYF54_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9832695.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2713..3489
                     /locus_tag="IYF54_002404"
     CDS             2713..3489
                     /locus_tag="IYF54_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ9832696.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3482..4051
                     /gene="rnmV"
                     /locus_tag="IYF54_002405"
     CDS             3482..4051
                     /gene="rnmV"
                     /locus_tag="IYF54_002405"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ9832697.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4044..4928
                     /gene="rsmA"
                     /locus_tag="IYF54_002406"
     CDS             4044..4928
                     /gene="rsmA"
                     /locus_tag="IYF54_002406"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ9832698.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5379..6008
                     /locus_tag="IYF54_002407"
     CDS             5379..6008
                     /locus_tag="IYF54_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832699.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYF54_002408"
     CDS             complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYF54_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ9832700.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7450..7872
                     /gene="mgsA"
                     /locus_tag="IYF54_002409"
     CDS             7450..7872
                     /gene="mgsA"
                     /locus_tag="IYF54_002409"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ9832701.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8195..8731
                     /locus_tag="IYF54_002410"
     CDS             8195..8731
                     /locus_tag="IYF54_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ9832702.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2766 a         1543 c         1790 g         2636 t
ORIGIN      
        1 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
       61 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
      121 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
      181 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
      241 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
      301 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
      361 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
      421 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
      481 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
      541 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
      601 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
      661 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
      721 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
      781 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
      841 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
      901 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
      961 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
     1021 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
     1081 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
     1141 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
     1201 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
     1261 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
     1321 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
     1381 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
     1441 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
     1501 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
     1561 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
     1621 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
     1681 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
     1741 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
     1801 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
     1861 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
     1921 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
     1981 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
     2041 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
     2101 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
     2161 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
     2221 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
     2281 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
     2341 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
     2401 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
     2461 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
     2521 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
     2581 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
     2641 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
     2701 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
     2761 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
     2821 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
     2881 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
     2941 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
     3001 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
     3061 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
     3121 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
     3181 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
     3241 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
     3301 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
     3361 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
     3421 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
     3481 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
     3541 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
     3601 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
     3661 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
     3721 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
     3781 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
     3841 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
     3901 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
     3961 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
     4021 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
     4081 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
     4141 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
     4201 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
     4261 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
     4321 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
     4381 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
     4441 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
     4501 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
     4561 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
     4621 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
     4681 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
     4741 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
     4801 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
     4861 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
     4921 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
     4981 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
     5041 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
     5101 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
     5161 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
     5221 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
     5281 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
     5341 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
     5401 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
     5461 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
     5521 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
     5581 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
     5641 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
     5701 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
     5761 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
     5821 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
     5881 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
     5941 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
     6001 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
     6061 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
     6121 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
     6181 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
     6241 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
     6301 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
     6361 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
     6421 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
     6481 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
     6541 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
     6601 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
     6661 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
     6721 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
     6781 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
     6841 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
     6901 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
     6961 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
     7021 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
     7081 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
     7141 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
     7201 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
     7261 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
     7321 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
     7381 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
     7441 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
     7501 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
     7561 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
     7621 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
     7681 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
     7741 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
     7801 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
     7861 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
     7921 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
     7981 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
     8041 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
     8101 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
     8161 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
     8221 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
     8281 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
     8341 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
     8401 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
     8461 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
     8521 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
     8581 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
     8641 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
     8701 tttaagaagt ttagcgatcc acgctaaata aaaaa
//
LOCUS       DACIWG010000080         8603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.084, whole genome shotgun sequence.
ACCESSION   DACIWG010000080 DACIWG010000000
VERSION     DACIWG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.084"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYF54_002411"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYF54_002411"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ9832703.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IYF54_002412"
     CDS             complement(1098..2201)
                     /locus_tag="IYF54_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ9832704.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IYF54_002413"
     CDS             complement(2291..3226)
                     /locus_tag="IYF54_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ9832705.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IYF54_002414"
     CDS             3528..5462
                     /locus_tag="IYF54_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ9832706.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IYF54_002415"
     CDS             5919..6335
                     /locus_tag="IYF54_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ9832707.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IYF54_002416"
     CDS             6349..6915
                     /locus_tag="IYF54_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ9832708.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IYF54_002417"
     CDS             6938..7924
                     /locus_tag="IYF54_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ9832709.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IYF54_002418"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IYF54_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ9832710.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1658 c         1750 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DACIWG010000081         8424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.085, whole genome shotgun sequence.
ACCESSION   DACIWG010000081 DACIWG010000000
VERSION     DACIWG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.085"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            471..2107
                     /locus_tag="IYF54_002419"
                     /pseudo
     CDS             471..2107
                     /locus_tag="IYF54_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYF54_002420"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYF54_002420"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ9832711.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="IYF54_002421"
     CDS             complement(3183..3818)
                     /locus_tag="IYF54_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ9832712.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYF54_002422"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYF54_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ9832713.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="IYF54_002423"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="IYF54_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6416..>8424
                     /locus_tag="IYF54_002424"
     CDS             6416..>8424
                     /locus_tag="IYF54_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ9832714.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDSFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1548 c         1292 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacagcttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaat
//
LOCUS       DACIWG010000082         8228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.086, whole genome shotgun sequence.
ACCESSION   DACIWG010000082 DACIWG010000000
VERSION     DACIWG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.086"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IYF54_002425"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IYF54_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ9832715.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IYF54_002426"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IYF54_002426"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ9832716.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IYF54_002427"
     CDS             2591..3376
                     /locus_tag="IYF54_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9832717.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IYF54_002428"
     CDS             3389..4240
                     /locus_tag="IYF54_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ9832718.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IYF54_002429"
     CDS             complement(4364..4816)
                     /locus_tag="IYF54_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9832719.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IYF54_002430"
     CDS             complement(5281..5649)
                     /locus_tag="IYF54_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ9832720.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IYF54_002431"
     CDS             5799..6281
                     /locus_tag="IYF54_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ9832721.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IYF54_002432"
     CDS             6318..7328
                     /locus_tag="IYF54_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ9832722.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IYF54_002433"
     CDS             complement(7443..8156)
                     /locus_tag="IYF54_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832723.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACIWG010000083         8046 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.087, whole genome shotgun sequence.
ACCESSION   DACIWG010000083 DACIWG010000000
VERSION     DACIWG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.087"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(98..1228)
                     /locus_tag="IYF54_002434"
     CDS             complement(98..1228)
                     /locus_tag="IYF54_002434"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ9832724.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IYF54_002435"
     CDS             complement(1877..3079)
                     /locus_tag="IYF54_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9832725.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IYF54_002436"
     CDS             complement(3139..3342)
                     /locus_tag="IYF54_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9832726.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IYF54_002437"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IYF54_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IYF54_002438"
     CDS             4879..5691
                     /locus_tag="IYF54_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9832727.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IYF54_002439"
     CDS             complement(6715..6867)
                     /locus_tag="IYF54_002439"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832728.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IYF54_002440"
     CDS             complement(7058..7936)
                     /locus_tag="IYF54_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ9832729.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         2457 a         1647 c         1254 g         2688 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagta
//
LOCUS       DACIWG010000084         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.089, whole genome shotgun sequence.
ACCESSION   DACIWG010000084 DACIWG010000000
VERSION     DACIWG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.089"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYF54_002441"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYF54_002441"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ9832730.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IYF54_002442"
     CDS             complement(2032..3273)
                     /locus_tag="IYF54_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ9832731.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IYF54_002443"
     CDS             complement(3291..4214)
                     /locus_tag="IYF54_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ9832732.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IYF54_002444"
     CDS             complement(4435..6927)
                     /locus_tag="IYF54_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ9832733.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IYF54_002445"
     CDS             complement(6934..7401)
                     /locus_tag="IYF54_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ9832734.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IYF54_002446"
     tRNA            complement(7537..7610)
                     /locus_tag="IYF54_002446"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IYF54_002447"
     tRNA            complement(7668..7741)
                     /locus_tag="IYF54_002447"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACIWG010000085         7699 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.090, whole genome shotgun sequence.
ACCESSION   DACIWG010000085 DACIWG010000000
VERSION     DACIWG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.090"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            544..1107
                     /locus_tag="IYF54_002448"
     CDS             544..1107
                     /locus_tag="IYF54_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832735.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IYF54_002449"
     CDS             complement(1386..1673)
                     /locus_tag="IYF54_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9832736.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IYF54_002450"
     CDS             complement(1663..1992)
                     /locus_tag="IYF54_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9832737.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IYF54_002451"
     CDS             2297..2626
                     /locus_tag="IYF54_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832738.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYF54_002452"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYF54_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ9832739.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IYF54_002453"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IYF54_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ9832740.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERVPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IYF54_002454"
     CDS             complement(4890..5498)
                     /locus_tag="IYF54_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ9832741.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYF54_002455"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYF54_002455"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ9832742.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IYF54_002456"
     CDS             complement(6528..7496)
                     /locus_tag="IYF54_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ9832743.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2351 a         1532 c         1280 g         2536 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg actcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatt
//
LOCUS       DACIWG010000086         7625 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.091, whole genome shotgun sequence.
ACCESSION   DACIWG010000086 DACIWG010000000
VERSION     DACIWG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.091"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(14..706)
                     /locus_tag="IYF54_002457"
     CDS             complement(14..706)
                     /locus_tag="IYF54_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9832744.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IYF54_002458"
                     /pseudo
     CDS             791..967
                     /locus_tag="IYF54_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IYF54_002459"
     CDS             complement(1143..1712)
                     /locus_tag="IYF54_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9832745.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IYF54_002460"
     CDS             complement(1786..2109)
                     /locus_tag="IYF54_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832746.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IYF54_002461"
     CDS             complement(2263..3075)
                     /locus_tag="IYF54_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832747.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IYF54_002462"
     CDS             3429..4277
                     /locus_tag="IYF54_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ9832748.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IYF54_002463"
     CDS             4422..5363
                     /locus_tag="IYF54_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ9832749.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IYF54_002464"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IYF54_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacct
//
LOCUS       DACIWG010000087         7600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.092, whole genome shotgun sequence.
ACCESSION   DACIWG010000087 DACIWG010000000
VERSION     DACIWG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.092"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(137..427)
                     /locus_tag="IYF54_002465"
     CDS             complement(137..427)
                     /locus_tag="IYF54_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832750.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="IYF54_002466"
     CDS             complement(545..805)
                     /locus_tag="IYF54_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832751.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="IYF54_002467"
     CDS             complement(942..1355)
                     /locus_tag="IYF54_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ9832752.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="IYF54_002468"
     CDS             complement(1811..2296)
                     /locus_tag="IYF54_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832753.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="IYF54_002469"
     CDS             complement(2435..2650)
                     /locus_tag="IYF54_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ9832754.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IYF54_002470"
     CDS             2874..3674
                     /locus_tag="IYF54_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9832755.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IYF54_002471"
     CDS             3693..5561
                     /locus_tag="IYF54_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9832756.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IYF54_002472"
     CDS             5554..6045
                     /locus_tag="IYF54_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ9832757.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IYF54_002473"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IYF54_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ9832758.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="IYF54_002474"
     CDS             6605..7600
                     /locus_tag="IYF54_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ9832759.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DACIWG010000088         7158 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.093, whole genome shotgun sequence.
ACCESSION   DACIWG010000088 DACIWG010000000
VERSION     DACIWG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.093"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(560..1600)
                     /locus_tag="IYF54_002475"
     CDS             complement(560..1600)
                     /locus_tag="IYF54_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ9832760.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IYF54_002476"
     CDS             complement(1949..2275)
                     /locus_tag="IYF54_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832761.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IYF54_002477"
     CDS             complement(2262..3050)
                     /locus_tag="IYF54_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9832762.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="IYF54_002478"
     CDS             complement(3418..3708)
                     /locus_tag="IYF54_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ9832763.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="IYF54_002479"
     CDS             complement(3844..4023)
                     /locus_tag="IYF54_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832764.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="IYF54_002480"
     CDS             complement(4020..4370)
                     /locus_tag="IYF54_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832765.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="IYF54_002481"
     CDS             complement(4363..5688)
                     /locus_tag="IYF54_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ9832766.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="IYF54_002482"
     CDS             6138..6752
                     /locus_tag="IYF54_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832767.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..>7158)
                     /locus_tag="IYF54_002483"
     CDS             complement(6917..>7158)
                     /locus_tag="IYF54_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832768.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2188 a         1292 c         1062 g         2616 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctat
//
LOCUS       DACIWG010000089         7035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.094, whole genome shotgun sequence.
ACCESSION   DACIWG010000089 DACIWG010000000
VERSION     DACIWG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.094"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            541..2133
                     /locus_tag="IYF54_002484"
     CDS             541..2133
                     /locus_tag="IYF54_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ9832769.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2146..2886
                     /locus_tag="IYF54_002485"
     CDS             2146..2886
                     /locus_tag="IYF54_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ9832770.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            3054..4403
                     /locus_tag="IYF54_002486"
     CDS             3054..4403
                     /locus_tag="IYF54_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9832771.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4531..5121)
                     /locus_tag="IYF54_002487"
     CDS             complement(4531..5121)
                     /locus_tag="IYF54_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ9832772.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            5268..6620
                     /gene="brnQ"
                     /locus_tag="IYF54_002488"
     CDS             5268..6620
                     /gene="brnQ"
                     /locus_tag="IYF54_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ9832773.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         2099 a         1286 c         1428 g         2222 t
ORIGIN      
        1 aattttctgt tctagttctt tttttattgt attacataca tttttgtgta taattatact
       61 atcgtttgca atggatagat gattcacatt ttgattgttt tttcggtaaa agtaaagatt
      121 tctatattta tcagtatttt ttcaaaaacc agcagttata gattgacaaa gcctctcgag
      181 tatcgtaaac tttttttgta taaataactg aatacgagat gcctcaatca atgaggtaga
      241 ggtcgcgaga atcattaacc ctgtggagtg aagcaaacat ggagaagcag gaatagggag
      301 gatcgccgaa atgtgcactt ttttgcttgg agtgtatgtt gggacgcaag ttaagagctt
      361 gcggactgtc ttagcagtga tgctaagttg cgctatgttt tgtgagaggt ttatcacctt
      421 atggaatttt agcggccttt ttatatctat aaatcggtcg ctttttattt tttcagataa
      481 agctttaccg catttcatct agaccgctcg tgtatcatat aatcagaaag ggagaaaaaa
      541 atgaaaaaaa atatggtttc agttgccgta ctatttgctt ttgtcttctc actattcagt
      601 gtgggatctc tggcaaatgc agatgaaaaa acaccgagcg atgagtttcg cgtaggtatg
      661 gaggctgggt atgcaccctt caactggtct caacaaacag atgagaataa tgccttgcca
      721 attcaaggcc aaaatagtta cgccggcggt tatgatgttc aaatcgctaa aaaagtagca
      781 gatggtttag ggaaaaaatt agtcatcgtc caaacgaaat gggatggtct ggctcctgcc
      841 cttcaatcag gcaagatcga cgcgatcatc gccggaatga gccctactgc tgaacgacga
      901 aaagaaattg attttaccga tccttattac gaatcacaat tagtagtagt agttcaaaaa
      961 gatagcaaat atgcaaaagc aaaaagttta gcagatcttt ctggagcaaa gatcactgct
     1021 cagttaaata cattccacta tactgtgatc gatcagatac cagatgtgca gaaacaacaa
     1081 gcaatggata atttctcagc aatgagaaca gctttagctt ctggaatgat cgatggttat
     1141 gtcagcgaac gtccggaagg tgtaacggca actagtgtga acaaagactt ggaaatgctt
     1201 gaattcagcg gtgcagatgg cttccagaca aacccggaag atgttcagat ttctgttggt
     1261 atgcgtaaag gcgacccgga tatccaaaaa gtaaaccaga ttctttctgg catctctaac
     1321 gatgaacgaa ccaaaattat ggaccaagca gtcaaagatc agcctgctgc aactgatagt
     1381 gaagaaaaac ctggcatcat ttctgatttc actacgattt ggaaacaata cggcaatatg
     1441 ttcttacgag gtgctggact taccttgttc atcgcattga tcgggacagt tgtcggtact
     1501 acacttggat tactgattgg tgtcttccgt tcggtacctg aatcaggaaa tgcactgact
     1561 cgcttcttcc aaaaaatcgg aaatgcactt ctttctatct atgtcgaagt attccgcgga
     1621 acaccgatga tggtacaagc aatggtcatc ttctacggtc tggcactcgc ttttggcatt
     1681 tcattgaacc gaacgattgc cgctttgttt attgtctctc tcaatactgg agcttacatg
     1741 acagagatcg ttcgtggggg gatttttgca gtagatgaag ggcagttcga agctgcgcaa
     1801 gccattggta tgacccatgg ccaaacgatg agaaaagttg ttattccaca agttttacgg
     1861 aatatcttgc cagcaacagg aaatgagttt gttatcaata tcaaagatac agcagtttta
     1921 agtgtaatcg gcgtagctga tttgttcttc caaggaaact ctgcttccgg tgcgaatttc
     1981 caattcttcc aaacatttac gatcgtcggg atcatctact tagtcatgac ctttacgatc
     2041 accagaatcc tgcgggcggt agaaaagaaa atcgatggtc cttcagctta taccaagctt
     2101 gaagaaatcg acaattcaaa tttgcaggaa taaggaggaa gaatcatgaa tgaaaaaatt
     2161 attgaaatca aacatctaag aaaaagcttc ggtgaaaatg aagtattgaa agatatcagc
     2221 gtaacagtca ataaaggtga agttgtgacg atcatcggtt cttccggttc tggtaaatct
     2281 actttcttgc gctgtatcaa tttactggaa aaaccaacag gcggcgagat catttataat
     2341 ggagaaaatg tattagctcc taaatataat ttacctaaat atcgaacgaa tctaggtatg
     2401 gttttccaat ccttcaacct gttcaacaat atgaatgtct tggaaaactg tatgtctggt
     2461 caaacaactg tcctgaaacg agataaagaa actgccaaaa aagtagcaat cgaaaatctg
     2521 gaaaaagttg gtatggatcg ttatatcgaa gccaaaccag cacaattatc tggtggacag
     2581 aaacagcgtg tcgcaattgc tcgggcatta tcaatgaatc cagatgtatt gctattcgac
     2641 gaacctactt cggcgttaga ccctgaaatg gttggcgaag tgcttaatac gatcaaagac
     2701 ttagcacata ctggcttgac aatgcttatc gtgacgcatg agatggaatt tgcaaaagat
     2761 gtatctgacc gtgtcatttt tatggataaa ggtgtgattg cagaagaagg tacaccagaa
     2821 gatatctttg tccatccgaa agaagaacgt acaaaagaat tcctttcacg tattttaaat
     2881 gcttaggatc aatagctgat tgggacaaag agctgcggtg tttaccgcag ttctttttta
     2941 tgaattactc ataaaaggta aatttctgaa aattttggaa tcttagttgt gtttttcaga
     3001 aaactctctt aatatggaag taattaaact tttttagtta ggatgattta aaaatgaatg
     3061 aagagcaaga actgcagcgt ggactgaaaa atagacatgt ccaactgatt tctattggcg
     3121 gagcgattgg aacggggttg tttttaggtt ccggaaagtc tatccagcta gctggccctt
     3181 cgatattgct cgcctatctt attactggat gcatctgttt tttcattatg cgtgcgttag
     3241 gcgaacttct gttatcaaac accaataacc attcttttct ggattttgtt gcagaatatc
     3301 ttggcaaaaa agcggctttc atcactggct ggacgtattg gttctgctgg gtatcgattg
     3361 caatggccga tttaacagcc atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc
     3421 agtggcttcc tgaactgatt gcattaggtc ttcttctttg cttgaatatg gtggctgtct
     3481 cattgtttgg tgagttagag ttttggtttg cattgatcaa agttgtagcg atcattgcct
     3541 ttatcctcgt aggagcttat atgatcctca cccactatac gacagatatt ggcacagcca
     3601 gtatcacgaa tctatggtct catggcggat tctttccaac tggtgcaaaa ggatttatct
     3661 tagctttcca aatggtaacc tttgcttttg ctgggataga attagttgga ttagtcgctg
     3721 gagaaacaga aaatcctgaa aaagtgttgc cggaagctat caataatatt cctatcagga
     3781 tcatcttatt ttacttagga tctctttttg tcattatggc aatctatcca tggaacagct
     3841 tgaatccaga taacagtcca tttgtagaag tattttcgga aattgggatc acgattgctg
     3901 cttccttgat caatttagtc gtattaagtg cagcagcttc tgcctgcaac agtgcaatat
     3961 atagtacagg acgtatgctt cgttctttag cgcaagaagg cagtgcccct aaaaaattca
     4021 aaaagttaac aacacatcat gtgccaggga atgctttgac attttctact atcgtcattt
     4081 tcatttctgt tatcttgaat tacgtgatgc ctagtgaagt attcacacta gtaagcagta
     4141 ttgccactac ttgtttcttg tttatctgga gtatgcttgt ctacacgcac atgaaatatc
     4201 gtaaaagtat cctcggcaaa aaagctcatt catttaaaat gccgttgtac ccatttagta
     4261 attatctggt atttctttac atggcctttg tgtgtgtcgt cttgttccta ggaaaagata
     4321 cgagaatcgc tttattacta acacctgtgt ggttcttgct tctattacta atctatcata
     4381 tgaaatatga acgtaagaag taagaaagta gttgtgacag aagtggtcag cttcgagaaa
     4441 taagaaggaa tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttcttg
     4501 gagataaaag cttctgtccc agcctctttt ctatttcccc ggaaacaacc gctgataata
     4561 ggcatctgct tttttatagg aaaaaatcca aataccagat acaaaaaaac gtccgaataa
     4621 cccgatcaac ggtacaaaaa agacaccggc aatcaagcat aagttaatca tcatggagat
     4681 aagacttcga cggtcctttg acaaaactct cacgacatgg gaatctggtt tatttgcttt
     4741 catgatttgc ctcatcaaga taccaaaaga aaaactccac atcgtgtata ctaagacata
     4801 agccatctca ggccaaaaag cttcagggtt acgcccgacc caactagtaa cagaaggaca
     4861 taaagtcagc caaaagagca aaaagatatt cgaccagacc actcggtaat taattcgttc
     4921 tgccatctga aatatacgat gatggttcac ccagatcacc gcaacaaata taaagctgat
     4981 ggtataagct aataatgtat ttcggatgac ccataaagaa tgaaagctag atgtattagg
     5041 gatcttgatc tctaatacaa taactctcaa aacaatggct accactccat cagtaaatgc
     5101 ttctaatctc gactttgtca cgatgattcc tcctcagatg attcagcgct tttattttat
     5161 cacttacgtt attaaataac catcgtttta tatagggaat tttctgaaaa ttgttgcata
     5221 tttttcgaaa ttttgttact tttaaaaaaa ccgtaaagga gttatgtatg acaaaaaaat
     5281 tatcatttaa agactatctt tttataggct ccatgctttt cggtctattt tttggtgctg
     5341 gaaatcttat ctttcccgtt catttgggtc aagaagcagg cgctgctacc ttttgggcga
     5401 atctaggttt cttggttacc ggtatcggtc ttcccttctt aggggtgatt gccattggtg
     5461 tatcgaaaag ctcaggtgta tatgaattag caaaacggat aaatcaaaca tatgcgatgg
     5521 tttttaccat cttgctttac ttagttatcg gaccgttttt tgctttacct cgccttgcaa
     5581 ccacatcttt cgagattggt ttagcaccat ttatcgatcc tagcagacag acgctatatc
     5641 tagcaatttt cagtattgta ttttttgtgc tggcttggtg gttttcaaga aaaccgacaa
     5701 agatattaga ttatgttgga aagttcctga atcctgcatt tttggtttta ttaggtgctt
     5761 tgcttctcct tgcttttttg aatccactag gttctgtaaa tcaagcgcct attcagccaa
     5821 attatcaaga gcatgctttt ttcacagggt ttactcaagg atataacaca ttagatgcgc
     5881 ttgctgcttt agcatttggg atcatcattg tcacaacaat ccaaaacatg ggtgtaacaa
     5941 aacctgctga aatcgctaaa gacacgatca aatctggtgc gatcagcatt atattgatgg
     6001 gaatcattta tactttgctc gcttatttag gtgctatgag cttaggtagc tttgctctaa
     6061 gtgaaaatgg cgggatcacg ctggctcaga ttgctgatca ttatttaggt acttatggca
     6121 gtatcttatt agcatttatc gttatattag cttgcttgaa gaccggtatt ggtttgatta
     6181 cagcttttgc agaaacattc acagatttgt ttccgcaaag aaattacgca ttctttgttg
     6241 cacttgctag tatccttcct tgtttagcgg caaacgtagg cttgacgaat atcattcaat
     6301 tttcgttgcc tgttttgatg ttcatctatc cattagcaat gacactgatt ttactagtgt
     6361 tagttggtcc tttgtttaag cagcgtacgg ctgtttatcg aatgactact tattttacat
     6421 tgattgcttc gatttttgat gggttgaacg cttgcccaga aactatcaaa caaacaccta
     6481 ttgttcaaaa tcttttatat gttgcggaaa gctacttgcc gtttttcaaa ttggggatgg
     6541 ggtggatcgt accagcagtt atcggttttg ttatcggact gatttggagt ttcgctaaga
     6601 aagaagaagt agcagattga taaaagaggt tgtgaaagag acgttcagcc ttgagcaata
     6661 aaaagtaatt ttgaaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga
     6721 aactgacttt tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat
     6781 taagaaatat tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga
     6841 gaaactggct tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa
     6901 acaaaacggc aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag
     6961 aagctgatca aacaacattt cttttataat atagatttta gacatatata tgatataaaa
     7021 atagttttcg caaaa
//
LOCUS       DACIWG010000090         6594 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.095, whole genome shotgun sequence.
ACCESSION   DACIWG010000090 DACIWG010000000
VERSION     DACIWG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.095"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(31..978)
                     /locus_tag="IYF54_002489"
     CDS             complement(31..978)
                     /locus_tag="IYF54_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ9832774.1"
                     /translation="MSERISYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1156..1959)
                     /locus_tag="IYF54_002490"
     CDS             complement(1156..1959)
                     /locus_tag="IYF54_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ9832775.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2105..3019
                     /locus_tag="IYF54_002491"
     CDS             2105..3019
                     /locus_tag="IYF54_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ9832776.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3067..3537
                     /locus_tag="IYF54_002492"
     CDS             3067..3537
                     /locus_tag="IYF54_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ9832777.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3622..4230
                     /locus_tag="IYF54_002493"
     CDS             3622..4230
                     /locus_tag="IYF54_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ9832778.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4364..4564
                     /locus_tag="IYF54_002494"
     CDS             4364..4564
                     /locus_tag="IYF54_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9832779.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            5318..6544
                     /locus_tag="IYF54_002495"
     CDS             5318..6544
                     /locus_tag="IYF54_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ9832780.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2228 a         1001 c         1317 g         2048 t
ORIGIN      
        1 tttttctttt gaaaatattg ttttcagcta ttaatatcct gcagacaatt ttacttgatt
       61 ggtttgaagc tcatgggtgg caaagtagct tttcagattg gttaaaaaaa tagccattaa
      121 tttcttttga aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta cattgtccat
      181 tttccaaaga ggatgatctt gtggaagagg ctcttcctca aaaacgtcta atgctgcaaa
      241 ggaaagttgc tggttattta aagcctgcac cagatcttgt gtcttaacag aagcaccacg
      301 gccaacattg atgaatacag attttggatc aaagtgttca aacatatgct gatcaaagag
      361 cccagttgtt tcctccgtta gaggtaagat attgactacg aaatctactt caggaagaac
      421 ttcctttagt tgatttaaag aaaaagtctt ttccattcct tctgctggat gaccggatgt
      481 attgattcca tagcagttca tgtctaatgc tttgcatttt tttgcaatca tttttccaat
      541 atggccagtt ccaatgatta gtattttttt cccttggata gattggtaat ggaccgatga
      601 acctaaccat ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac gagaataccc
      661 aaggatgatt cctaagacat gctccgtaat cgattcactg tggatgccac taccatttga
      721 aagtaaaata tgtttctttg aaaaagtctc aaggggtagc tgatcaacac cagctgagat
      781 agattggacc catttcaagg tagaagaggc taatagtttt tcttggtagt ctgagttcca
      841 gcccaaactg atctctacat tttgtagatc ctctggttgt aattctgttt taaagatata
      901 accaggcgct atagtatgga tttcttcgat aaattcatca cgaaatggac gatctgcata
      961 actgattctt tctgacatag ttaatttccc gcctttcttt actctaagtg tagcgcatac
     1021 aagaagaact ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt caaataaaaa
     1081 aggggctgtg acaaaaaaat gtcacaacct ctaaaagggt ctattgaagt agaaataacg
     1141 agttgtcttt atttcttaat tgatcgatat ttttgcccat cctttgacag cttgagcaaa
     1201 aataaagatt cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa gaaagaccat
     1261 caagattgtt gcaattatag taggcaatga aattgaatag atcaatgttt ttaaacaatc
     1321 aagaaaagta gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa tgttggtaaa
     1381 tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa atgtcaggac
     1441 ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct tacctgttaa
     1501 gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca acaaagcaga
     1561 agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc cgacactaac
     1621 aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct ctggatcaaa
     1681 tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg tttgaagctg
     1741 cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat ccaattgaat
     1801 gttgtttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta tactcaaaac
     1861 taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta ggtctttgaa
     1921 tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc atctagtagt
     1981 gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta aggaaacagc
     2041 tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat ccaatataaa
     2101 taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa aactatttgg
     2161 agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga ttatgtcagg
     2221 gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg caattatttt
     2281 ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta cgtatggcta
     2341 ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca tattatcctt
     2401 gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa atagtcattt
     2461 gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat tattattacg
     2521 acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt taagtgatac
     2581 actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg atatctactg
     2641 gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag cgataatggt
     2701 gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctatcgact atgaaaaaat
     2761 ggaacaagaa attgaagcaa tcgatggggt aaaggatgtt catcatgtcc atatttggca
     2821 gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc agctactaag
     2881 cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat atggtattac
     2941 acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa tttttttaaa
     3001 taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa taaggtaggt
     3061 tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg atattgggag
     3121 atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt tagttttgct
     3181 ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc gtggttttgt
     3241 tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa atcaccgaat
     3301 tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct ttcttacgga
     3361 ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga gtatgtggcg
     3421 aaaatcatca ctcaaattat tgttatcctt gcgaactata ttttcagcaa gttgtttatt
     3481 tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga cgactaggca
     3541 taacaattga agaatttcct tctctttgtt actctttgta atgtagacat tattaagaaa
     3601 acattgagga ggaatttttt tatgacttac acattaccag atctacctta tgcatatgac
     3661 gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa gcatcacaat
     3721 acttatgtga cgaatttaaa ttcagcaatt gagaaatacc cagaattagg cgaaaaaaca
     3781 atagaagaat tattatctga tatggacgct attccaacag atatcaagac agctgtacgt
     3841 aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc accaaatgct
     3901 ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg tgatttttct
     3961 tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg atgggcttgg
     4021 cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga ttctccattg
     4081 atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta ttatttgaaa
     4141 tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa ttgggatgaa
     4201 gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca gtcttaaaaa
     4261 taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg tataatgttc
     4321 ttgcaaggtt acttgcaaga acttttttgg aggatataca tttatggaac acggaacggt
     4381 taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg gagatgacgt
     4441 atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag aaggtcaaga
     4501 agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag tgactaaatt
     4561 ataataattt gttgaataaa gacacgactt cttagagagt cgtgtctttt tgtctattta
     4621 tgaagttttt agattttttg gctaagtaaa tgcatttgtt cacttgacat gtcctaacta
     4681 attatcaagc aaggaacatt ttcttcgtta gtaatggatt ttaaagaaat ttcagttttt
     4741 ggatgttgaa taaaagacca tttgtgaaac cactagtata catactcatt accatgtcaa
     4801 ttctattact ttaaaagaaa atcgttttac tttgaagagg agcatatcta tccattaaaa
     4861 gatgatggta atgcaatcac tagttcgagt cattttaaga gccatgcagt gagctggaaa
     4921 taaaacgaat agaagatggg tttaacaaaa ggaccagtca taggaagaaa ttgtcgtgaa
     4981 atcgaatgat aatctgaacg gtaggcaaag actgttaaaa agatgggaaa ggtatgtgcg
     5041 atcattttac tgcacgatta tttggaaaat accttgttgt tcgctttttc tggaaaaaat
     5101 acataaaaaa agataaaacg attgcgaact gcgtgtattc ggtctttcga tctttttcac
     5161 ggtatggtat gatagttaag aattttgaga aattttaaaa aataataatc gttacacaaa
     5221 aatgtttttt aaagttttct tatgattaaa aagtgaattt gctgattgaa cagttatatg
     5281 agacaatatt ttagaaaatg tgaaacagag gtgtattatg aagcgatggc aaaaagttct
     5341 attaaccttg ttaggggtat tcgttttgct gattgccgga ttatcagcat acggaatcag
     5401 atttatggga gaagcgagcc aaacagtaaa caaaatttct aaaaactcta atcgaatatc
     5461 ctccaaacgt tctgagagag taagcattga tgacaaagaa cctttttcgg ttttgctatt
     5521 aggtttagat accggtggat taggaagaac ggaacaagga cgttctgata cgatgatggt
     5581 agtaacagtt aatccgaaac aaaaaaaatc aacgatcatc agtttggacc gagatattta
     5641 tacgaatatc gtaggttatg gaacggtaga taaattaaat catgcctacg catttggtgg
     5701 tgtcgaaatg gccatggatt caattgagca actgctcgat attcctattg atcattatgt
     5761 aacgatcaat ttggatggca tggaagattt gatcaatgcg gtcggtggca tcaaagtgaa
     5821 taataaaatt gactttactt tggatggtgt ccatgtacca gtaggtgaac aaacattgaa
     5881 tggagaaaaa ggtttagcct attctcgaat gcgacacgaa gatccagaag gcgatatcgg
     5941 tcgacaacgt cgtcagcggg aagtagtgac gaaaatcgtc aataaagttt taagtttaga
     6001 tggcgtaagc aattatagaa aaattttaaa agcagttgag aacaatgtga cgacggactt
     6061 agattgggat gacatgcttg atgtagcgac gaactacacc ccggcgtttg aaacaatcaa
     6121 acaagaccag ttgcaaggcg aaggacaaat gatcaatgat atctactatc aaattttagg
     6181 aaaaaatgat cttctttcga tccaaaatga actgaagaaa caattaaata taaaaacaag
     6241 cgatacatta cctaatttga aaaatgagaa tgcttcaatg atgttctttg atgattcgga
     6301 agatggcgat tcgacaaacg gtgatggatc gaatagtgaa gggtacaccg attattcttc
     6361 ctattcagaa aacacatatg atcagtacca agatccgaat acctattatc agcaaacaga
     6421 agatacaagt cagtactata caccaagtca agatactcaa agttatgatc cttacgctgg
     6481 acaggaacaa aatggttatg gttatgacca gaataatgag tatggaacaa caagtgggta
     6541 ctagaaaaaa atagtttggt taataaagag tgccacctaa attagacaaa aaat
//
LOCUS       DACIWG010000091         6238 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.096, whole genome shotgun sequence.
ACCESSION   DACIWG010000091 DACIWG010000000
VERSION     DACIWG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.096"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(315..602)
                     /locus_tag="IYF54_002496"
     CDS             complement(315..602)
                     /locus_tag="IYF54_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9832781.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(622..846)
                     /locus_tag="IYF54_002497"
     CDS             complement(622..846)
                     /locus_tag="IYF54_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ9832782.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1486..2019)
                     /locus_tag="IYF54_002498"
     CDS             complement(1486..2019)
                     /locus_tag="IYF54_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9832783.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2040..2984)
                     /locus_tag="IYF54_002499"
     CDS             complement(2040..2984)
                     /locus_tag="IYF54_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ9832784.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3621..3707
                     /locus_tag="IYF54_002500"
     CDS             3621..3707
                     /locus_tag="IYF54_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ9832785.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3828..4517)
                     /locus_tag="IYF54_002501"
     CDS             complement(3828..4517)
                     /locus_tag="IYF54_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832786.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4524..4802)
                     /locus_tag="IYF54_002502"
     CDS             complement(4524..4802)
                     /locus_tag="IYF54_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832787.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4762..5676)
                     /locus_tag="IYF54_002503"
     CDS             complement(4762..5676)
                     /locus_tag="IYF54_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ9832788.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5658..6038)
                     /locus_tag="IYF54_002504"
     CDS             complement(5658..6038)
                     /locus_tag="IYF54_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ9832789.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1649 a         1173 c         1055 g         2361 t
ORIGIN      
        1 cgtatttggt gcaaataacc gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc
       61 ccccccactc tccacaatac aaaaaagact agcaaatgct agccactttt tgtaaagttt
      121 cgcttgtttc accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt
      181 ttcgttgttc aaagattctt atgccattta ctattaataa atctataaat atgataattt
      241 ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat
      301 tttattccaa agaattaact ccaaatacca atagaagccc atcctctata tacttgagat
      361 tccttactaa caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt
      421 aaaatatatc ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc
      481 tcactgattt ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata
      541 gttgcttcat tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc
      601 attataataa cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc
      661 attaacaata atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc
      721 tttgttacaa taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc
      781 gatgccagct agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt
      841 tttcatgatt gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta
      901 gtaattttat tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt
      961 tgaattcata atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat
     1021 aatgtgtata ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt
     1081 tttattggtc taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct
     1141 aattctttgt gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg
     1201 ttatgctttt tacatatctc atattcattt attattcttt tctaaggtta ataataggat
     1261 aatctctttc agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta
     1321 caaatacttc ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt
     1381 tatttccttt gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga
     1441 tagaaccgaa tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg
     1501 ccaccatttt ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt
     1561 cgttgaacta ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt
     1621 ttcgatttgt tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat
     1681 atgagaatta ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg
     1741 caactgatct agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc
     1801 gttgttttgt tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc
     1861 aagttgcaat actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat
     1921 ggaagaatct aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc
     1981 tgacaattcc tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc
     2041 tactggaata aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg
     2101 gactctgttt gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg
     2161 agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg
     2221 tattcttttt gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca
     2281 tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata
     2341 acaggttttc cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca
     2401 gttagttctt gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt
     2461 ttaggaatat ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt
     2521 ctgtattgct ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat
     2581 tgttgtaaag aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg
     2641 taaattcgta tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac
     2701 attacaaaca tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag
     2761 attttttcat aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag
     2821 ttacttaaat cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc
     2881 attctagaca cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga
     2941 attgtattta attcatggtg gtattttaca atctcattag ccatataatc acctcaatcc
     3001 aattatgaat gacatttttt aaatgtcaag acattcatat ttgacgttca aacagacaaa
     3061 atgtccttca taaacagaca aaatgtcctt cataaacaga caaaatgtcc ttcataaaca
     3121 gacaaaatgt ccttcataac attctgtatc cattggtaga gtaaggacac agcttacggt
     3181 aaagaggttt aaaaagaggt ttaaaaagag atataaaaag aggggggcta attttcccgc
     3241 ctaaaaacca cttctttttt ctttaaacag gttcgcgcat ttttcaaaac tgccaatcca
     3301 aaaccttgct tcctacggtc gccaaaaagg tgtgatcgct acgctctcac attgtactgc
     3361 gcagtcgcct aaaggctcct ttgctaaaaa catgttggta aagaggcctt tgagcggttc
     3421 tgaatgctta tctaagccgt tttaagacag tcttggataa aaatccttat ctagctttaa
     3481 agcgccttac aggcggtcag ggcaagccct aacaacccta aaataagcga tacttaaaaa
     3541 caccacacaa gtttaatttt ttaagatata ataacttgtc gcctatccga cagtcttgcg
     3601 actggaagga ggtgtatcta atgtttgaat tgttcatttg cccccttctt atagaagttt
     3661 gtgtctcttt attcagttat tggttgaata accgaaacaa gaagtaaaat caataaaggc
     3721 gacatcaacc tgaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtatcta
     3781 atgtttgatc atttgcatgt ttagtttaac atgattgccg atcaaattca attttgctgt
     3841 tgctgatcta accattcctt gacacttttt agataattta atgccgtttc ttttttcgtt
     3901 ggattttgta tgttcgttgc ttgctgaagc agtgctttat aacgttcgta ggtaactgat
     3961 tgttgccgtt cttttcgttc ctgttcacta gttgaaaaag ggattgtatc actgaataaa
     4021 ttataacaag cttcttctgt ctgcgtatct ttaaaatatt gcacgtcttt tttggttcgc
     4081 tcatagttgt cttgaacctc tttttgctgt ttttctggca agttttgata aagacgagtg
     4141 agtttttcca tgtttttatc cgtcaaacgt ttttcttgat tcacaaaaat atcgttcatt
     4201 tcctcataac aagtggtaaa ctcttccatt ttttgtgtta acacttgctc ctttttcttt
     4261 ttatcacatg ctgcttcata cttttgagtg atatccccta ataaaatact tcttgaaata
     4321 cgtttatttc cctctaaaac gccgaataaa ccacttaaaa cgattgataa tgtaaatata
     4381 acccctggca ataaaaacct cttaaatcga attttagagc cttttaatga atatcttgtt
     4441 tgataagcct tatagctaag ccaaataatt cctaaaatac tggcaaaaat gagtagatag
     4501 ggtaaataga atgccatttc tatttattct ccccatacca gccaaaaggc taacagtgtc
     4561 atgattaagc aacataaggg ggcaagaata tccattgatc gctgcataat tacttttttc
     4621 tgttctttat ttcgctggtt taatagcgca tggtggaatt ttttgacttg tgaatcaatt
     4681 gtagtttgta ttctttcttt ggtataaggt gcaagaaaag cacgttgttt ctttatttca
     4741 ttttctatgt cttgtaacgt tttagaacga taattcttgt tggtagtctg ctcctctttc
     4801 aacgtgttgg tcatggtttc ttcgagtcgg tctaatttcc ggttgttcag tcgtaagagt
     4861 tcgttgttct cttcgtactt cttttgcagt ttcgcgaatg ctagctcgag ctcttcgtag
     4921 ctcgttggtt cgttgttgat tgtaggctcg ttctcgtctg tccagctcat gtaaaatcgt
     4981 ctccttttcg taatcctttc ctagtttgtt ggatcgtact tttttctttt tgtggatcaa
     5041 ttcgtaagta aaatttttcc cacgattcaa gcaatgaatg ccgaatgttt taagagaatc
     5101 acaaaattgg ttatacgttc gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg
     5161 aatttcgtct ttccaaacag gttcattttt ttcttttaat ttgatttcag acatggttcg
     5221 tttttcgggt ttcttccctc gttcagtgat tgttaagtca tgttcaagac aaatctcgtc
     5281 atttaggtca taacatttgt ttaaaaatgc ttgaccatgg gcttgaaatt tgttcccagt
     5341 ttccagattg acggagttaa tgacaatgtg gttatgaata tggtctttgt ctgtatgtgt
     5401 gtaaatcgcg atttggtggt caggggctat ttttttcgca tactctgccc ctagttgatt
     5461 ggctttttca gcggtaattt ctcctggtcg aaaagactgg atcaataagt gtgcttgtac
     5521 acgatcgtct tttccataca gcatgcgaat ttgtttcatt tcgcttttgg cataatcaat
     5581 gtcacagttt atccctgttt tgacggttgc tcgtggttcg gcgtaattga tcgctcggct
     5641 acaagaagtg gttcggctta ctttaactgt tgccatatct tttgtagctc cttttgcatg
     5701 atttttaggt tttcggataa gcgttgggcg tcgccaggat caacgtcgtg ttggtgtgtg
     5761 ttgcaccact tggcaatttg attgatattc ccgccagctt tggctaaatc gcgcgcgatt
     5821 gcctgagcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg tgccttggct
     5881 ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag ctttgcaaat
     5941 tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt atatttttta
     6001 gaatgattgc tagccaaata ttgattttgt tcgctcataa gtcccaccct ttcagtacgt
     6061 cctacggacg tatcgttcgc aataccattg tacctaaaaa ggtacaataa gtaaagctca
     6121 ctaaagctca ctaaagggct tggcaagctc tatatttgct agccaaatac ctccgtattt
     6181 ggtgcaaata accgcttgtc aaaggggcgc aagctccccc ttgaatcccc ctcaaaac
//
LOCUS       DACIWG010000092         6061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.097, whole genome shotgun sequence.
ACCESSION   DACIWG010000092 DACIWG010000000
VERSION     DACIWG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.097"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            825..1559
                     /locus_tag="IYF54_002505"
     CDS             825..1559
                     /locus_tag="IYF54_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ9832790.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1575..2744
                     /locus_tag="IYF54_002506"
     CDS             1575..2744
                     /locus_tag="IYF54_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ9832791.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2760..3755
                     /gene="lacD"
                     /locus_tag="IYF54_002507"
     CDS             2760..3755
                     /gene="lacD"
                     /locus_tag="IYF54_002507"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ9832792.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3834..4760
                     /locus_tag="IYF54_002508"
     CDS             3834..4760
                     /locus_tag="IYF54_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ9832793.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4779..6029
                     /locus_tag="IYF54_002509"
     CDS             4779..6029
                     /locus_tag="IYF54_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9832794.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          996 c         1243 g         1796 t
ORIGIN      
        1 cagcgaatcc ttgataatga gctcggatgt gctaacgcta gcgaggcagt ctaactttaa
       61 ctgttagctc ctgccgaaga tcgtcacatc cgaggaggct cattgtcaag gacaatcgga
      121 atagcatacg gaaagaagtt ctgcacctta taaaatttgt caaaaaaact gttgccattc
      181 caaagtttga aaaatgctat ttcgaggtag aaggatatta tataagcacg gttgactaaa
      241 cgaagtgata ataaaaaaag ttattcaaga ataagaaaaa ttatgatacg aattcagata
      301 aactggagaa tacagagacc tttaaaaggg ataagaaaca acataaattc ctcttaagag
      361 agaacgaaag gacaaagcca gcaacttgat acacgagcta gtattagagg aatttattct
      421 catagactcc cttttgaata cgtttcaact ttaattttca tgttcataaa tgatttgaaa
      481 ccaaagcaaa tgcgttgcac ttttcctccg tcttccgaaa atcgttcaat tcactggtaa
      541 gtagtcattt gatagtttga ggcaaattgt tgattgatga agggtaggag ctaaaaaaag
      601 ataccagttg aagagagaaa aagaaaaaaa tagagggtgt tcttgaatga aaaaagttgg
      661 aaaattattt ttcatttcct tttattccta ttaaagtcag tgatgtcaat catattaaca
      721 agactaaaaa tgaaaataaa aaaaagattg ttcttagtat tgactcataa aaaataccac
      781 tactatactt acattgtggt tatagccagt taggagagac atcgatgcaa aatacctttc
      841 aatcaaaatc actttattat cagctggtag aaatattgaa acaacgaatc gaaaacagta
      901 tggtcgctca tgacaagctg ccatcagaaa gagagttgac cagtttttat ggcgttagtc
      961 gcacgacggt gcgactagca ttgcaagaac tggaaaaaat gggctacatt tatcgacaac
     1021 atggaaaagg aacctttgtt tcggaattaa ggacaaatgc gactgatcta gctggcgcat
     1081 atagttttac cgaacaaatg cgggcaatgg gcaaacatcc tgaaaccaag atattggcct
     1141 tcaagaagat tgaagcaact aaatatttag cgagtcatct gaatgttcaa ctaggtgagc
     1201 ctttatttca attagaccga ttgcgcttag ctgatgattt gccaatgatg atcgagaaat
     1261 cctatttgcc agtgaagcta tttatgtccc tttctcaaga aattctattt ctgaaacctt
     1321 tatataattt gttttcagag aatttcaatc aaatcgtacg gttggctgac gaggaatttt
     1381 atgccagcat tgtccagaaa gaagatagtg agttgctaaa tattccagaa ggatcaccag
     1441 tgctccattt agttcgctca acctataatc tcgagaacga agtgattgaa tttacattaa
     1501 gtgtagcgcg ggctgataaa tttcattata agatcaggca tctgcgttcc actaactgaa
     1561 ttggaggaaa gaaaatgttt atgttctcaa aagaagaact acgaaagcta ggagcagaaa
     1621 atactacccg agaaattcga caacaaccgg aactgtggca tgagacgttt gaccgttatc
     1681 aagaaacagt tgcagattta gatcgtttgt ttacggatgc gaaacaacat gctggagagc
     1741 gaccaatccg agtcattttt acaggggccg gaacctcgca atatgtggga gatacactaa
     1801 ttccgtttct tactagtcaa ggaaatacga accagtttat ttttcaaagt attggttcga
     1861 ccgatatcgt tgctacgcca aaagaatacc tgattcctga tgagacaacg atcttggtct
     1921 catttgcccg tagtggaaat agtccagaaa gtctagccgc agttaagatg gccaatcagc
     1981 tggtcaacaa gatctatcat atcgtgatca cctgtgcacc agacggatta ttggctacac
     2041 aaatgcctac aaaaaaagaa aatctagttt tactaatgcc aaacaaagcc aatgacgctg
     2101 gttttgctat gacaagcagt ttcagctgta tgacgctgac tgcgctactt atttttgatc
     2161 aagcaagcaa tcagcaaaaa gaaagatatg ttcatcaatt aagccgacta ggagaagaag
     2221 tcatccaacg agaatcagag atccaagggt atctggcatc agactttcat cgcatcattt
     2281 acttaggttc gggaagctta ggcggattga ctagagaagc gcaattaaaa gttctcgagt
     2341 taactgctgg aaaaatcgca accgtatttg atacatcttt aggattcaga catggtccca
     2401 aatcttttgt caatgagcaa acattggttt tcctgtttgt cagcaatgac tcatatacgc
     2461 agaaatacga tgtagacatg ctggaagaaa tcaaaaatga cggtattgcc caacgcgttg
     2521 ttggaattgg tcaaaagggt tcattgttta gtggtgattc ctttgaatta gttgcagatt
     2581 cgttactgcc ggatggctat cttgctttag cagatatcat ggtagcccaa acgatcgcat
     2641 tactttgttc ggtgaaggtt ggtaatacac cagatacacc atcgccaagt ggaacagtca
     2701 atcgtgtggt caaaggcgta acgatttatc catatgaaaa ataggaaaga gtgtgaagca
     2761 tgttagaatg gtcggaaaat aaaagacaag caatggaacg tctatcgacg ccagaaggca
     2821 ttattaccgc tttggcgatc gatcagcgag gcgcgctgaa gaaaatgatg tcggctttag
     2881 ggatggaagc gacaaaggaa aaaattgagc agtttaaaac actaatttcc caagaattga
     2941 ctccttatgc ctcagcaatt ttattggatc ctgaatacgg actgcctgct gcacagaagc
     3001 gtgcagcagc gagcggtctg ctgttggcat atgaaaaaac tggttatgat caaaccgtac
     3061 ccggccgctt gccggatctg ctgtcggact gttcagttct gcgattgaaa gagttaggtg
     3121 cggatgcggt gaaattttta ctgtattacg attatgaaga agcagaggaa atcaatcggc
     3181 aaaagcaagt ttttgttgaa cgaattggaa gcgaatgcat ggctgaagga ctgccatttt
     3241 atttagagtt attgtcatat gatgctaaaa atgtagatac gaaatcgctt tcctatgcca
     3301 aagcaaaacc gcgcaaagtg atcgaaatga tgaaagaatt ctcgaaaacc cagtataaag
     3361 tagatgtctt aaaggttgaa gtaccagtaa acatgtcatt tgttgaagga aatgcaacag
     3421 gggagatcgc ctatacacgt caagaagctg ccgcttattt taaagaacaa agcgatgcaa
     3481 cagatttgcc gtatatcttc ttaagtgctg gggtatcagc ggcattattc atggagacat
     3541 tgcgttttgc caaacatgct ggggcacgat tcaacggtgt actctgtggc agagcaactt
     3601 ggaaagatgc agtcgctcct ttcgttgaaa aaggagaagc ggcaacttta gaatggctga
     3661 cagaacaagg gacacaaaac attcgtcaat tgaatgaagt gataagagaa actgccatcc
     3721 catggtatga gaaagtaagt gaaagcactt gttaatgttt ctaatatatt agtagtgata
     3781 agagatatgt gtgattataa tttatcatta ttgaaactgg gaggatattt tatatgaata
     3841 aaagattgtt agattattca gcttcagatt ttttgaaagc taagccgatg gaattaaagc
     3901 aggcaatttt ggcttctgaa ggtagaacaa ttatggcaga aaatgtgcca agtagctgtc
     3961 cttacatggg aaaatcagtg acaaatgcag aattggagag agctgctgga gctgatttaa
     4021 ttttgtttaa tgcactagat ctttttaatc ctcaaatttc aggtgtacct ttaactctaa
     4081 aagagaatcc aataatctgg attaaaaaag ctttaggaag agctattggt gtaaatttag
     4141 aacctgtaga caaagaagtt ttgatgagtg aaaatcgaac tgaaatttca agtggtagaa
     4201 tcgtttctcc tgagacttta aaaaaggcaa atgaattagg ctttaatttt atttgtttaa
     4261 ctggaaatcc aggaacagga gtatctaata gaactattga gaaagcaata acattatcca
     4321 aatcaatttt taatggatta attatcgctg gaaaaatgca tggtgctggt gttgatgaac
     4381 caattatgaa cttagatata gcaaaaagat ttattaaaac aggtgtagat attttattag
     4441 ttcctgctcc atacactgtt ccgtatttca tggaaagtga tttgcgagga atcgttgatt
     4501 atataaaaga ttttaataga aataaatcta ttgaggaaaa agttctagta ctaacggcta
     4561 atggaacaag ccaagactct tctgaccaag aaactattaa aaaaattgca ttagcatcaa
     4621 aagcatgtgg agctgatatt caacacatag gggattcttt aaatggtatt tgtcttccag
     4681 aaaatatttt tgctttgggt caagcaattc ggggatatcg tcatcaaatt attatgttag
     4741 gaaaatctaa ttatcgttaa ttaaggagga aatgaaagat gatcgacaaa gttcaaaaaa
     4801 tagctgcaaa aattagtaat caaagacatc tactagcttt aagagacagc atggctgcag
     4861 ttatgccatt aatcattatt ggttcctttt ttatgttatt agcaaatttc ccgatagaag
     4921 gatttacaaa atggttatct gaaattggag tacaatcctt tttgaacaaa gcatcagatt
     4981 caacatttgg tattattgga ttagctgtta cctactcttt gggacacaat ctagcaaaga
     5041 cttatggtgt agactcatta tcagcaggat tattatcttt gagtggatat gttttactta
     5101 cacctttatt ggatgcagat ggttctatag gatttccaac aaaatacttt ggtacctcgg
     5161 gtctttttgt tggtattatt gtagcacttg tatctacaga aattttccgg tggtttgtac
     5221 ttaaaaatat tacaattaaa atgccggcaa gcgttccacc aaatgtaagc cgagctttcg
     5281 ttgctattat tcctggcttt tttgtcgttc tactatggtt tattgttgtt gtaatttgtt
     5341 ataaacttgg aattgaaaat gttcatgctc ttattgcaga tacactcgca aaaccactat
     5401 ctttactgac gaaaacttta ccaggaatta tattggttat acttattcaa tgtttctttt
     5461 ggatgtttgg tattcatggt gcacaggtaa cggggccaat aattgaaccg ttattattgc
     5521 aaaactctga tgtaaataga attgcttatc aagctggaaa agaacttcct aacattataa
     5581 cttatgaatt cttatataac tttgtttttt ctggtggtgc aggttgttta tttgcacttg
     5641 ccattttaac atttttcttt tcaaagagtc aggaaaataa aactttaggt aagttatcaa
     5701 tcgctccagt aagttttcaa gtagcagagc cccttctatt tgggtttcca actatattaa
     5761 atataaaaat gttaattcca tttgtaactg caccagtagt aacaacatta attacttatt
     5821 tttcaatgag tatgggatta gttgcaaaac ctgtaggagc aacaatccca tggacaactc
     5881 caccgataat tgccggattt ttagcttctg gtggacgaat aagtggtgca gtgattcaag
     5941 taataacaat tgcaataaat gttttgatat actatccatt ctttaaatta gatgataatg
     6001 ccaagctaaa aagcgaaaaa aatgattaga actacctata tgtttctaac tgtagttaag
     6061 t
//
LOCUS       DACIWG010000093         6058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.098, whole genome shotgun sequence.
ACCESSION   DACIWG010000093 DACIWG010000000
VERSION     DACIWG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.098"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            292..2523
                     /gene="pflB"
                     /locus_tag="IYF54_002510"
     CDS             292..2523
                     /gene="pflB"
                     /locus_tag="IYF54_002510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ9832795.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2767..3528
                     /gene="pflA"
                     /locus_tag="IYF54_002511"
     CDS             2767..3528
                     /gene="pflA"
                     /locus_tag="IYF54_002511"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ9832796.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3613..4539
                     /locus_tag="IYF54_002512"
     CDS             3613..4539
                     /locus_tag="IYF54_002512"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ9832797.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4721..5614
                     /locus_tag="IYF54_002513"
     CDS             4721..5614
                     /locus_tag="IYF54_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ9832798.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5748..5873
                     /locus_tag="IYF54_002514"
     CDS             5748..5873
                     /locus_tag="IYF54_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832799.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         2143 a         1022 c         1195 g         1698 t
ORIGIN      
        1 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
       61 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
      121 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
      181 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
      241 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
      301 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
      361 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
      421 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
      481 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
      541 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
      601 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
      661 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
      721 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
      781 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
      841 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
      901 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
      961 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
     1021 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
     1081 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
     1141 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
     1201 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
     1261 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
     1321 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
     1381 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
     1441 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
     1501 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
     1561 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
     1621 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
     1681 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
     1741 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
     1801 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
     1861 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
     1921 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
     1981 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
     2041 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
     2101 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
     2161 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
     2221 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
     2281 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
     2341 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
     2401 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
     2461 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
     2521 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
     2581 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
     2641 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
     2701 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
     2761 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
     2821 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
     2881 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
     2941 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
     3001 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
     3061 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
     3121 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
     3181 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
     3241 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
     3301 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
     3361 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
     3421 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
     3481 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
     3541 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
     3601 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
     3661 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
     3721 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
     3781 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
     3841 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
     3901 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
     3961 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
     4021 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
     4081 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
     4141 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
     4201 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
     4261 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
     4321 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
     4381 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
     4441 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
     4501 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
     4561 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
     4621 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
     4681 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
     4741 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
     4801 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
     4861 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
     4921 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
     4981 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
     5041 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
     5101 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
     5161 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
     5221 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
     5281 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
     5341 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
     5401 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
     5461 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
     5521 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
     5581 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
     5641 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
     5701 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
     5761 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
     5821 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
     5881 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
     5941 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
     6001 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaat
//
LOCUS       DACIWG010000094         6025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.100, whole genome shotgun sequence.
ACCESSION   DACIWG010000094 DACIWG010000000
VERSION     DACIWG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.100"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            234..899
                     /locus_tag="IYF54_002515"
                     /pseudo
     CDS             234..899
                     /locus_tag="IYF54_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            935..1180
                     /locus_tag="IYF54_002516"
     CDS             935..1180
                     /locus_tag="IYF54_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9832800.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1283..2152
                     /locus_tag="IYF54_002517"
     CDS             1283..2152
                     /locus_tag="IYF54_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ9832801.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2133..2867
                     /locus_tag="IYF54_002518"
     CDS             2133..2867
                     /locus_tag="IYF54_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9832802.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2900..3808
                     /locus_tag="IYF54_002519"
     CDS             2900..3808
                     /locus_tag="IYF54_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ9832803.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3805..4347
                     /gene="sat4"
                     /locus_tag="IYF54_002520"
     CDS             3805..4347
                     /gene="sat4"
                     /locus_tag="IYF54_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ9832804.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4440..5234
                     /locus_tag="IYF54_002521"
                     /note="aph(3')-IIIa"
     CDS             4440..5234
                     /locus_tag="IYF54_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ9832805.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5342..5530)
                     /locus_tag="IYF54_002522"
                     /pseudo
     CDS             complement(5342..5530)
                     /locus_tag="IYF54_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5510..>6025
                     /locus_tag="IYF54_002523"
     CDS             5510..>6025
                     /locus_tag="IYF54_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9832806.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2070 a          899 c         1324 g         1732 t
ORIGIN      
        1 aaaagcccta caatgccgtt gtaaaaattg gggaaaaggg atatattgat tttatctctt
       61 tttcaaaata aacatggggc tgacccaaaa gtagctccta tatgaaaaat agaggtcttt
      121 ccatttagat agtagtggaa agacctctat ttatcttttg aaagtgaata caaaaataaa
      181 gcacactctg ctataatata ggtgattaat ccatttgaga gaggtgcttt atcatgtatc
      241 aaaattatac cataaaccaa ctttgtttgc ctatagatct tgaaattaaa ttagaagaaa
      301 atgattttgc tcatgccatt gtgcagtttg ttgactctat tcccgatgaa gtctttcttc
      361 cttattatca atctatgggt agaccccaat atcatcctcg tatgctactt tccattattc
      421 tttgtgccta tattcaaggg gtttattcgg gccgccaaat acaaaatatg ctgatagata
      481 gtattcggat gcgctactta tctcaagagc aattccctaa ctttcgtact attaatcgtt
      541 ttcgcgttca tccgattatg aacgatattc tagatcattc atttgttcaa tttcgtgagc
      601 tactggttca aagtggctta atcagtggca gtgctctata cattgatggt acaaaaattg
      661 aagcggatgc gaataaatac tctttcgttt ggaaaaaaag tattttaaag tataaggata
      721 gccttgatca aaaagccctt gaaaactacc aacaaatggt ggaaacaaaa atcttacctg
      781 agttaataga tgatttaaag gatgaacttt ctatagaaga gattgaaagg attcgtcaat
      841 ctttggaaga aaaggaaaat caattaattg aagcgattga ccaaaccgaa acagtagaag
      901 ggggctgacc cattattaat aaggagtgga caacatgatt gatgtaaatg agctattgga
      961 agaagctatt agagaaactg aaaatctaaa tgatggcgaa gtttttcttg ttaaagattt
     1021 attcaaggga tatgtgggga ataggatacc cagaaaggat cgacttctgc ttggaacgtt
     1081 atttttaaac catgttagca aaatgaatgg taatttaaag gctattgaaa agacttcatc
     1141 taatcagcag aggtataaaa agacaattga taagttttaa ctatcttttg tgaaattaaa
     1201 aaaattataa atatgttgag tagaagcaaa agatttggct tggaattttt acagcagtga
     1261 atcgataaaa ggagcatgct tgatggtaga taatattatt aaatcagtag cagagaaatt
     1321 atcctctctg tcttatatag aaggtattgt tttaggtggt tcacgtgcaa ggggcaccca
     1381 tacagaggat tcggatatag atatcggcat ctattacaat tcagaatcat ttgacataaa
     1441 tactattaat caattcgcta caaagctgga tgatgagcat agaaataacc ttgttgtacc
     1501 tcccggagca tggggtgatt ggattaatgg cggcggatgg ttagtcataa acgggtatca
     1561 tgtggattta attttacgtg atattaaacg tgtggaacaa ataatgaaag atacagagca
     1621 cggaattgtt actgccaatt atcagactgg gcatccccat ggttatatta gtgcaatgta
     1681 tcgaggagaa ttagcgatta gcaaaatact atatgctaag aatgaaagct tatgcgaatt
     1741 aaaaaaacag gcagaaactt atcccaatgc tttgcagaaa agtttagtta acttttttat
     1801 gtttgaagca gggttctctt taatgtttgt aaaagcaaat tcgggaacag acgataaata
     1861 ttatattgcg ggtcatgttt ttcgtatagt ttcatgttta aatcaagtgt tatttgcatg
     1921 taataatgct tattgtatca acgaaaagaa agctataaaa ctgcttgaaa cttttgaaca
     1981 taaacctgaa aaatataccg agaaggtaaa tcatattttt gaagtactcg gtatctcact
     2041 ttttgaatgc tacgacatga ccgagaagct ttataatgaa gtgaatgaaa ttgtatcgga
     2101 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     2161 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     2221 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     2281 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     2341 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     2401 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     2461 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     2521 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     2581 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     2641 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     2701 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     2761 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     2821 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     2881 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     2941 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     3001 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     3061 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     3121 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     3181 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     3241 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     3301 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     3361 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     3421 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     3481 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     3541 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     3601 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     3661 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     3721 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     3781 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     3841 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     3901 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     3961 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     4021 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     4081 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     4141 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     4201 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     4261 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     4321 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     4381 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     4441 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     4501 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     4561 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     4621 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     4681 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     4741 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     4801 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgattag
     4861 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     4921 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     4981 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     5041 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     5101 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     5161 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     5221 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     5281 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     5341 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     5401 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     5461 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     5521 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     5581 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     5641 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     5701 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     5761 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     5821 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     5881 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     5941 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     6001 agaaccggaa agcagaatga tagag
//
LOCUS       DACIWG010000095         6006 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.101, whole genome shotgun sequence.
ACCESSION   DACIWG010000095 DACIWG010000000
VERSION     DACIWG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.101"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..386)
                     /locus_tag="IYF54_002524"
     CDS             complement(<1..386)
                     /locus_tag="IYF54_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ9832807.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPT"
     gene            complement(383..2482)
                     /locus_tag="IYF54_002525"
     CDS             complement(383..2482)
                     /locus_tag="IYF54_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ9832808.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(2533..3354)
                     /locus_tag="IYF54_002526"
     CDS             complement(2533..3354)
                     /locus_tag="IYF54_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ9832809.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(3351..4160)
                     /locus_tag="IYF54_002527"
     CDS             complement(3351..4160)
                     /locus_tag="IYF54_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ9832810.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(4181..4675)
                     /locus_tag="IYF54_002528"
     CDS             complement(4181..4675)
                     /locus_tag="IYF54_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ9832811.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(4687..5175)
                     /locus_tag="IYF54_002529"
     CDS             complement(4687..5175)
                     /locus_tag="IYF54_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ9832812.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(5252..>6006)
                     /locus_tag="IYF54_002530"
     CDS             complement(5252..>6006)
                     /locus_tag="IYF54_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9832813.1"
                     /translation="FKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKD
                     VFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYD
                     ILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETV
                     ISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSR
                     SIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         1836 a         1373 c          987 g         1810 t
ORIGIN      
        1 gtaggattaa ccactatcgg caacgagttc acctcgcaac gctctccaaa cccattacaa
       61 aagaaatccc ctaaaggact ttcaacagca gggctatccg acccatccca ataaattctt
      121 aagattaaat cacgtaaaac aaaactgcca aattctgttc gatcacgaat cgtcatccac
      181 atatgacgaa ttattcccgt tccagtaata tccgcaatca cagtttcttc accagaagtc
      241 agcggaattg cgccacgacc tttgcggctt ggtcctagct cgcttggcaa catcgcagct
      301 ttacctttct cgcctgttgg attttcggga gtgattgctc gactctggat ttttttaaat
      361 tgggtaatat tttttagtat actcatcttt ttctcctttg aatgtgaatc gtttttccct
      421 tttctacgtg aagaattttt ttcgtatcac ttattttcaa tggcttttct gacaaatttg
      481 aaatactcaa ctcggattgc gtacattcaa tcgaaatgaa ggcttgaccg aacttcaaat
      541 tcttccaaga aaacgttaaa ttatcaacag gatcaatttg caatactcct tttttgaatt
      601 tcaatccgaa atattgactg atcattaggc tgacaaaaag acccgatgcc cagccgcatt
      661 tgccgcagcc aaagtctcgc acgacttctc cttgttgagc ctctaagcga tacggccacc
      721 accaccacga accgtctaaa tctgccaaac aaattagttc ttctaatcgg gttacgaaag
      781 cttgagggtt gtgcagctcg ctcattaaaa cagtgataaa gccaggaaaa gtggcgccgg
      841 attctaatcc ccaagtgatg cctttgattt cctcgctata ggttggattg aaagaactag
      901 cagaaaaggc catggtgttc tgatataaat gatctttatt atctaaaaat tgataaaaag
      961 gcatcaacgt agtgtctgat tcttctccat catgcatcaa taaattaatt tttccatcca
     1021 caatcacatc acttaaaaag attaaccctt gctcaagaat cggtttttga tagtgcttca
     1081 ctgaataggt tgttttctcc tcatcaccga ttccctccaa aaattgctca ccaaaaggac
     1141 catcgatagt catttgttta caaattgatt cctttagttt acaggcaatg tttttgtatc
     1201 gcttgctaag actcagttgt tctagggctt caaaaatttc tgcaagtcca ctacaggcct
     1261 tccaaagaca aagattgctg ccggtatgat actttccaag tgcaaaggcg tctgataacc
     1321 aaacagatgg aaaaagcatt ggttcccctg accgacgttg agctaatatg tcctcaataa
     1381 ttcttgaagc attcagtaga acctgcggat accgctcaaa gaattttgta tccccagtat
     1441 gttcaaaata aagactagcc agtaacgcac tggataacga attactcaga gaatgattga
     1501 tacctcctgt aaattttgaa ccaggaaact taacgccata ttgatcaaac catagaaccg
     1561 ttttttgaca taactccggt tcaaacatgg tgaatggcaa tgatgcgtaa tacatatcct
     1621 tattccaaat tcgattagta gcaggatagc tgccccaatt gctgccgacg acctgtccct
     1681 gtcgattcat accgaaacta gaaaagcttt gataaagtgc acgacgatag agatggacaa
     1741 ctctatcgtt tggcattgac agctgcccaa atagctgagc aaaatagttc aatgtttcct
     1801 tcagccattt ttcgatatca gattcctgaa aagtcattac ttcttcaaaa gcatttgggt
     1861 ccacaaaagc aatggcgaag cttgccactt cctgttgtgc gatcgtgtcc ttatttcctt
     1921 tttgagaaat caacacattt tgctgatccg aatacttctc ttggtacaaa ggcacatctt
     1981 tcactgaaac ttgaaggctt tcgtgtgttt cattcacaat gtaaacctgt tgaacaagca
     2041 tacttaaacg cttattagcc agtattggag caaaggatac tacaatgacc ttaaacgtcg
     2101 aataatgatg aatatatcta ggcaaacagt cttgaatcac atcgacttca atacgatgat
     2161 ccgattctga caaatggtag acaatcccgt ttacttcaac ctttaaggat aacgaattgg
     2221 tttgagaaag ctgtttattg acccaaacac ctggtttttc ctcagttagt aaattttctc
     2281 gataaaaggt caacgtttga atcgtcccgc aaacatcaag gtttcctaat gtcttttgat
     2341 tacttacatc gaaggtcaat ccctttacat caggaatatt ccgatagtag ctgtattcgt
     2401 ttgaagtagt cacatttccg tcgtttaact cagcattaaa taatgggtgt ttgggtgaat
     2461 aaattacttc attaatagtc atcttttccc tccttgctaa aaaaactgca gcctagtagc
     2521 tgcagcttcc tactatgcga ttccaagtaa tgacaataaa attcccaaga cgaccattcc
     2581 aaaaataatt ttgatgacgc cgactttttt atctagtaat ttgaaacagc ccaatgttac
     2641 caataacggt acaattcctt taaaaatact atcgaggatt tcttggacat tcattagttc
     2701 ttcgcccttc attgaaaagc tcaaaacggt attgaatttg accatactga tcgtcattgc
     2761 gcctaccatg atcagaccca agatactagc agcttttgtc agaatttcaa ttaacccgtt
     2821 cgcatacagc ttttcaatat atttagagcc taatgaatag cctaaaacgc cgccatagta
     2881 tcgcatcaaa taattaggta tattgaacat aactaaaaag ataattggtg ccaaaatatt
     2941 tccggcattg gctaagttga tcgctagtcc cgcggtaacg acacgccaaa ctccccagaa
     3001 caatgaatcg ccaattccag ccaatggtcc catcaaactc gttttgatcg cgttgatact
     3061 ttccacgtca aaaccagctt tttcagaatt ttccttctcc atcgatgccg caatccccat
     3121 gacaaaggac gcgcaaggaa tggtcgtcga aaaatactgt gtatgacgtt ccagcgccgc
     3181 agctcgatct tcttcacttt tgtagaaatg attaatgaag ggcatcatag aataggcgaa
     3241 accaccgccg cccatcgtca ccggattcac cgaagcatac atagtcatcg agcgccaaaa
     3301 catcttccga accaattttt tctcatcttt agaaattcca tcagctaatt tcattgaaag
     3361 aaatcctcct cttcttggtc aagcgttgca tttacagcag catcgctttg aactgcatga
     3421 acagatatat tttttagttg tagatctctt tgggctataa caattgcaat aatcgttcca
     3481 actgcggcaa ttgcaatcaa tggtaactcg agatacgcca taacaacggc cccaaagaaa
     3541 aaccacacag caatattttt actccaaagc atccgtaaca gcatagccat cccaactgcc
     3601 ggcagcagat ttccagcgac tgttaatccg ttcatgatga cctctggaat attatccaag
     3661 acgcttttga caacatctgc tccaaataaa acggcaaaga atggaataag agcataaagc
     3721 gcatagtgga caaaccacat aacgaagtgc agattgatga ttcctttttg atcaccagat
     3781 tttgcaaatt tgaaaaattt agcagccatc ggtccacata caaaagaaaa aatcatattc
     3841 attaactgta atcctaatac tgctagtgga aagacaatgg gaataatcgc atccacgttt
     3901 cctcccattt tgattgcgaa cgctgtagcc agcccagcag ccaaagaagg ctccgccgta
     3961 ctagagattc cgatattgac gacacccata aagatggctt ctagtgacgc cccgacaatc
     4021 aaccccgtct gcaaatcccc catcaacgct cctgctaacg gagcaacaac aatcggacga
     4081 ctcagcatgg acatcccgaa catttcatgt ccagcaactc caataaaaac ggccaaagcg
     4141 actacaaatg catcatacat aatttttctc cttccttttt ctaaaaatca ctcaaaatag
     4201 agatagctga acgtttttcc atcgccggtg taggctgaaa aacggtatct ggataaagtt
     4261 caactaattc ttttaagtct tgaatttctg acgtcgttaa catcacttcc tttgataagg
     4321 ttttcttatc ttccgatggc ggtgtcattt tcccagcatt ccccacgttc attaattcga
     4381 tttccgttgg gaaagctttc gcaactttta ccgcatcttt gacattaggg actaaaacca
     4441 tcaagcgcat gtccttcgcc cgcggatctt ttaacaaatg aatcgcgtca tcaactgttt
     4501 taataatgac tttgacattc gatggtgcag ccatcttcaa tgtcatcttt tgaatatcgt
     4561 tggtagccgc ctcatcgctt gctactacta acccggttaa atttagactc tttgtccaag
     4621 tcatcgctac ttgtccatga attaatcgtt catcaattcg cactgcttta atcatttttc
     4681 ttcctcctaa aaaaaatctt cctcttcatt tactacatgt acctcatcaa gaatggtcat
     4741 ctttaattcc tgttggcatt tatttaccag ttcctgaata tcctcctttg ttaattcctt
     4801 ggttgccaat actatttcga gcagaattgg cagattaaag ccagacaaaa tagttacagc
     4861 aatctctttc tccaaaaagg ttttggtaat cttctgattg acacttccgc caaataaatc
     4921 ggtaaaaaca aaaatctgat cctgctcgtt cacctggttt acaaatacat tcaactcctg
     4981 ttcaaaatca cactcatcca cataagcatt aatcaccgta atctcttgaa ccttccccgt
     5041 cagtaattga atactgctaa gaaagcctga agctaagtga ccgtgagtcg ctattagaat
     5101 tttcttcata tggttaattc ctttctttct gttcactttt atataagcaa ttcctgtgcc
     5161 aacttttaaa aacattggtt taatcacgtt aattcacatt aaaaaaagtg tctaacctaa
     5221 aaaaaacaga ttagacactt ttttagacac tttatttttg aatatacggt cctaataatt
     5281 catagagcag cgaaagctca taagcattga ctttaaaatt atatttcatc tccattgatt
     5341 gaaaaatact tctagaaaca taataaaatt catcttcatc tggtttcaaa tcataattcg
     5401 gttcttcaga tttttctgtc cgagccaaga ataaacgttc catcatcaat gccgtatgca
     5461 tatagagatt tagttttaca cggccattta aagtgaaatg atagtaattt tcatatttag
     5521 aaatgactgt ttccacttca ctcatcacga tcgttgggtt cagaaagttc aaacggtccg
     5581 caattccttc caacgaaaag aattttacta gctcctgaat caagcggtta aattctgccg
     5641 gcttcatttc aggactcaaa atatgctcca aattggctgc tccatcaccg tctaagatgt
     5701 cataaacatt aatattagga attgaaaagg aacgtggaag atccgaagtc gtgatgacca
     5761 attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt agttctctgt
     5821 agtccttcgt gaaaacttcg atggattcat ccgataaatg atgacgaaaa acatctttca
     5881 atttgtccga aatccccaaa cccgacatac aggaaatgat aatgttgcta ctttgagaaa
     5941 agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt
     6001 taaatg
//
LOCUS       DACIWG010000096         6000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.102, whole genome shotgun sequence.
ACCESSION   DACIWG010000096 DACIWG010000000
VERSION     DACIWG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.102"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(65..934)
                     /locus_tag="IYF54_002531"
     CDS             complement(65..934)
                     /locus_tag="IYF54_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ9832814.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1226..2836)
                     /locus_tag="IYF54_002532"
     CDS             complement(1226..2836)
                     /locus_tag="IYF54_002532"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ9832815.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3058..3303)
                     /locus_tag="IYF54_002533"
     CDS             complement(3058..3303)
                     /locus_tag="IYF54_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832816.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFGTHKGLKK"
     gene            complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="IYF54_002534"
     CDS             complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="IYF54_002534"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ9832817.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(5397..5789)
                     /locus_tag="IYF54_002535"
     CDS             complement(5397..5789)
                     /locus_tag="IYF54_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ9832818.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1760 a         1281 c          988 g         1971 t
ORIGIN      
        1 agaatggaga gcgacttcaa aagtcgttct ccattcctat atatcgtatc tgttatgtgg
       61 atgcttacgc tttgttagct gaaccgaacc agtcgatatg agatttagca tcttcaacga
      121 ttgctgcttt acctggagct aataatttac gagggtcgaa tcctttacct tctttatctt
      181 taccagcttc gatatattta cgagtagctg cagcaaatga taattggaat tcagtgttaa
      241 catttacttt tgagataccc attgagatag ctttttcgat ttgatcttga ggaatacctg
      301 atccgccatg taatactaaa ggcatgtctg gacctacagc atcagcaatt gcttgtaagt
      361 ggtcaaaagc taatcctttc cagttttctg gatattgacc atggatgtta ccgataccgc
      421 aagctaagta gtcgatacct gtagcaacca tttgtttgca ttcttcaata tcagctaatt
      481 cgccgttacc gatgattccg tcttcttctc cgccgattga accaacttca cattcaactg
      541 agataccttt tgcatgtgct ttttcaacaa cgtctttagc taatttcaag ttttcttcaa
      601 atggtagatg tgaaccatcg aacattactg aagtatagcc taattcgata cattccaaag
      661 cagcttcgta atcaccatgg tctaagtgga tagcaactgg aacagtgatg ttcattgctt
      721 caactaaatc agtgattaaa tctttacaga ttttgtaacc acccatgtat ttagctgcac
      781 ccattgatgt ttggataagt actggtgctt gttttgcttc agccgcttca aggattgctt
      841 gagtccattc caagttgttt gtgttgaatc cgccgacagc gtaaccgcct ttacgagcag
      901 cttttaaaaa ttcagctcct gatactactg gcataataaa attcctccta agttttgtaa
      961 aactaaattt gttaagacaa ttcttaacca acgaccctat tttaccaaac atttcggtag
     1021 ttttctacta aaatcaatga aaatgaaaat tttcaagaag ttttttgcaa aaaataaaat
     1081 atccaatagc gctttaatcg aaaaaagtct tttgctttct atcttttttc ctacaaataa
     1141 taacaaatca gaaaaagaga aaagtgacaa gaaaattgct tatccccttc tcttgctact
     1201 tttctctttc tatcaagttc aaaaattatt ttgtttcgtg atctgctaat gatgctccga
     1261 caaatgcttt gattaatttt tgcgggcgat ttggacgaga aatcaattct gggtggaatt
     1321 ggcatcccac aaagaatttt ttctctggca gctctacgat ttctactaaa cggttatctg
     1381 gtgagacacc tgaaaagact agcccttgtt cttcaaacaa ttgacgatat ttgttattga
     1441 actcatagcg gtgacggtga cgttcttgca caacatcggc tccatcatat gcttcagccg
     1501 tcttcgttcc ttttttgatt ttacatgggt ataatccaag gcgtaaagtt ccacccaaat
     1561 tctcgatgtt ttcctgatca gccattaaat caataatatt gtttgctgta tctggattcg
     1621 tttcagctga tgacgcatct tctaatccaa cgacgttacg tgcaaattcg acacaagcca
     1681 tctgcatacc aagacaaata cctaaaaacg gaacgtcatt ttcgcgagca taacgaatcg
     1741 cttcgatttt cccttctact ccgcgatcac caaatccacc aggaaccaag attccatctg
     1801 cgtctttcaa acgttcttct acattttctc tcgttaattc ttgtgctttg atccaatcga
     1861 tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa tgcttcaact actgagatat
     1921 atgcatctgg taactcgaca tatttaccga caagcgcaat ccgtactttt ttcttcaagt
     1981 tcaagacacg ttcttctaat tctttccatt ctgtcatgtc tgcttctggt gtatctagtt
     2041 ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg taaaactaat gggatggagt
     2101 aaagtgtatc tacatctggc gactcgataa ctgcttctgg ttcgacatca caaaattgtg
     2161 ccaatttgtt tttcacgctt tgagaaacag gttgctctgt acgtaccacc aaaatattcg
     2221 gttggatacc taaaccacgt agctctttta cactgtgctg cgttggttta gttttcattt
     2281 ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat atacataaca ttatctgtac
     2341 cgacatctgc tttcatttga cgaagtgctt caaggaatgg caaagattct atatccccta
     2401 ctgttccccc tacttctgta atgatcacat ctgaatcagt cattttagct gctcgcatga
     2461 ttttttcttt gatctcattt gtaatatgag gaataacttg aacagttgct cccaagtatt
     2521 cgcccttacg ttctttacgc aaaacttcag aatagatttt tcctgtcgtc acgttggagt
     2581 atttgttcaa attgatgtca ataaaacgtt cgtagtggcc cagatccaaa tctgtttcag
     2641 caccatcatc tgttacgaat acttctccgt gctgatatgg gctcatcgtc cccggatcca
     2701 cgttgatgta tggatcaaat ttttggatag ttacttttaa gccgcggttt ttcaataagc
     2761 gtcctaaaga agctgcaaca atcccttttc cgattgatga aacgacaccg ccggtcacaa
     2821 aaatatattt tgtcataagt tcaaaaactc ctttttaatt tatgtgcagg aaaagtctca
     2881 gacaataaac aaagctccct attcttcaat aagaataggg agccgaaatt tctgtaaact
     2941 tctgaccctc taaaagggtg cccaaggtgt ataatacaag ctgactaagg gaaagtcaag
     3001 tactcaaatg aatactatcc gcttacaatt ttttcttttt cgatatcctt tgagaaatta
     3061 tttcttaagt cctttatgag tgccaaaaaa atttaaccca cataataagc ttgatataaa
     3121 catagctaac gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa
     3181 taatagataa aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact
     3241 actttttcct acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt
     3301 cacccttatt tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat
     3361 atcttttcta ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac
     3421 ctttttttca acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa
     3481 aaaataagga gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct
     3541 gccaactcct cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt
     3601 atttcttgtg attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa
     3661 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
     3721 atttttctta atactcgaaa ctaaacgtct ttttcaaaac ctcttggtaa acggtgttca
     3781 aaagcttgca tctgcggtaa taagttcagc caattcaaaa acaagaaaag ctgtttttgc
     3841 ttggctattc ttattacttg ttgcagggca gctttttcac aacctctcca ctaaacggtg
     3901 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
     3961 ccgttagttt ctctaatact caggtcaaaa cgctgatttc acagcctcta attcacggta
     4021 ttcctgtttt aataaagttc tagatattgt tctctttccc actcggaaac agtttgtctg
     4081 aatgccgccc attcgagacg ttttgcttca acaaaatttg agaagatatg ttgtcctaat
     4141 gcgtcaatca ttacattatc cttacgcaat tctttgatag cattatggat agttgaaggt
     4201 aagtcctgga tatgtgcagc ttctctttct tcctcatcca ttacataaat gttacggtca
     4261 acagctgctg gcggtgtgat ctgattacgg ataccgtcta gtcctgcttg caacaatact
     4321 gccattgcca agtatgggtt tgcagatgga tcaactgacc gcaattccaa acgtgtggat
     4381 aaaccgcgag attctggtac acgtactaat ggcgaacgat tacggccact ccatgcgaca
     4441 taaacaggcg cttcataacc tggaacgaga cgtttataag aattgactgt tggattgcaa
     4501 acggctgtgt aggcacgagc atgtttcagc aaaccaccta agaaatgata agctgtttca
     4561 cttagttcca attcaccgtc tttatcatag aaaacatttc cttcatcatt gaacaaagac
     4621 atattgcaat gcataccaga accattgatc ccaaataacg gtttcggcat gaaagtcgcg
     4681 tgaagtccat gcttgcgtgc aatcgttttg acaacgagct tgaaggtttg gatattatca
     4741 caagcatcta ccacatctgc atatttaaag tcaatttcat gctgtcctgg cgctacttca
     4801 tgatgtgaag cttccacttc aaagcctaga ctttctaatt ccaacacgat gtcacggcgg
     4861 caattttcac ctaaatctgt tggtgcaaaa tcaaaataac cacctttatc attcaaggtc
     4921 gttgtgatcc caccgtcttc gtctaattta aataagaaga attctggttc aggtcctaaa
     4981 ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg ctcgtttcaa attcccgcga
     5041 ggatcgccgg caaatggtgt cccatccgga ttatagatat cacaaattaa acgagcaact
     5101 tttccatgag cactttccca cgggaagatc atccaagtgg agagatctgg atacaaatac
     5161 atatcgcttt cttcaattcg aacaaatcct tcaatcgaag aaccgtcaaa catcatttta
     5221 ttttctaata ctttgtcgat ctggctcact ggaacttcta catttttgat cgtacctaag
     5281 atgtctgtga acatcaatcg taaaaatcgt acgttttctt cctcaatgat tcgtttgatt
     5341 tcttcacctg ttatctgttt ctttaccatg gtatccctct tcctttttta atttattcag
     5401 tcaaagaatt gtctttgcaa tccttgcgac tgataatgac ctgttttgct taacccacct
     5461 tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat gatctgtcaa cggcttcttg
     5521 ttttttgctt cttgtttttt cgcctgtgcc atttcgtata cacgtttgat cccagccata
     5581 ttcaatccct ctgaaagata gtctttaatc tcaagcaaca tatcaatatc attcagcgaa
     5641 tacatacgtc ggtttccttc gcttcgttcc ggatgaacta aatcttgttc ttcataatag
     5701 cgaatttgcc gagcagacaa atcagtcagc ttcataactg taccaattgg aaatacggac
     5761 atagatcggc gcaattcctt ttctcccatc agacgtcccc tttcgtttta taagttggct
     5821 aaagtgtacc aattaaaaaa aatattgtca actgcatatg ttaggttttc taacatgaga
     5881 tctctatttt ttataaaata acaaaaaaat aagaacacaa aaaaggtatc tagttgattt
     5941 tcgtaccaga taccttttat tttttattat actgtactat cctttattag tgtttttatt
//
LOCUS       DACIWG010000097         5988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.103, whole genome shotgun sequence.
ACCESSION   DACIWG010000097 DACIWG010000000
VERSION     DACIWG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.103"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(76..1170)
                     /locus_tag="IYF54_002536"
     CDS             complement(76..1170)
                     /locus_tag="IYF54_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9832819.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IYF54_002537"
     CDS             1359..2120
                     /locus_tag="IYF54_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ9832820.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IYF54_002538"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IYF54_002538"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ9832821.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IYF54_002539"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IYF54_002539"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ9832822.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IYF54_002540"
     CDS             complement(3760..4773)
                     /locus_tag="IYF54_002540"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ9832823.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IYF54_002541"
     CDS             4938..5858
                     /locus_tag="IYF54_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ9832824.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1830 a         1197 c         1076 g         1885 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtcccacg ctctaaacac
     5941 gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaat
//
LOCUS       DACIWG010000098         5954 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.104, whole genome shotgun sequence.
ACCESSION   DACIWG010000098 DACIWG010000000
VERSION     DACIWG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.104"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..813
                     /locus_tag="IYF54_002542"
     CDS             <1..813
                     /locus_tag="IYF54_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ9832825.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IYF54_002543"
     CDS             807..1229
                     /locus_tag="IYF54_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832826.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="IYF54_002544"
     CDS             2399..3367
                     /locus_tag="IYF54_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9832827.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="IYF54_002545"
     CDS             3384..3908
                     /locus_tag="IYF54_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9832828.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="IYF54_002546"
     CDS             4284..4565
                     /locus_tag="IYF54_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832829.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="IYF54_002547"
     CDS             4567..4983
                     /locus_tag="IYF54_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832830.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="IYF54_002548"
     CDS             5613..>5954
                     /locus_tag="IYF54_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ9832831.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DACIWG010000099         5727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.105, whole genome shotgun sequence.
ACCESSION   DACIWG010000099 DACIWG010000000
VERSION     DACIWG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.105"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            196..921
                     /locus_tag="IYF54_002549"
     CDS             196..921
                     /locus_tag="IYF54_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832832.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IYF54_002550"
     CDS             911..1420
                     /locus_tag="IYF54_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832833.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IYF54_002551"
     CDS             complement(1490..2938)
                     /locus_tag="IYF54_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ9832834.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IYF54_002552"
     CDS             complement(2938..3654)
                     /locus_tag="IYF54_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ9832835.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IYF54_002553"
     CDS             complement(3635..3985)
                     /locus_tag="IYF54_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ9832836.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IYF54_002554"
     CDS             4129..4902
                     /locus_tag="IYF54_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ9832837.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IYF54_002555"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IYF54_002555"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACIWG010000100         5698 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.106, whole genome shotgun sequence.
ACCESSION   DACIWG010000100 DACIWG010000000
VERSION     DACIWG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.106"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="IYF54_002556"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="IYF54_002556"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="IYF54_002557"
     tRNA            203..276
                     /locus_tag="IYF54_002557"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="IYF54_002558"
     tRNA            316..405
                     /locus_tag="IYF54_002558"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="IYF54_002559"
     tRNA            420..491
                     /locus_tag="IYF54_002559"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="IYF54_002560"
     tRNA            507..579
                     /locus_tag="IYF54_002560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="IYF54_002561"
     tRNA            589..661
                     /locus_tag="IYF54_002561"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="IYF54_002562"
     tRNA            697..772
                     /locus_tag="IYF54_002562"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="IYF54_002563"
     tRNA            774..854
                     /locus_tag="IYF54_002563"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="IYF54_002564"
     tRNA            861..934
                     /locus_tag="IYF54_002564"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="IYF54_002565"
     tRNA            937..1009
                     /locus_tag="IYF54_002565"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
     gene            1021..1092
                     /locus_tag="IYF54_002566"
     tRNA            1021..1092
                     /locus_tag="IYF54_002566"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1053..1055,aa:Gln,seq:ttg)
     gene            1138..1208
                     /locus_tag="IYF54_002567"
     tRNA            1138..1208
                     /locus_tag="IYF54_002567"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1170..1172,aa:Cys,seq:gca)
     gene            1229..1313
                     /locus_tag="IYF54_002568"
     tRNA            1229..1313
                     /locus_tag="IYF54_002568"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Leu,seq:caa)
     gene            1375..1447
                     /locus_tag="IYF54_002569"
     tRNA            1375..1447
                     /locus_tag="IYF54_002569"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1408..1410,aa:His,seq:gtg)
     gene            1457..1528
                     /locus_tag="IYF54_002570"
     tRNA            1457..1528
                     /locus_tag="IYF54_002570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Gln,seq:ttg)
     gene            1546..1630
                     /locus_tag="IYF54_002571"
     tRNA            1546..1630
                     /locus_tag="IYF54_002571"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1580..1582,aa:Leu,seq:caa)
     gene            1885..2760
                     /locus_tag="IYF54_002572"
     CDS             1885..2760
                     /locus_tag="IYF54_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9832838.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2864..3628
                     /locus_tag="IYF54_002573"
     CDS             2864..3628
                     /locus_tag="IYF54_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ9832839.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3645..5057
                     /locus_tag="IYF54_002574"
     CDS             3645..5057
                     /locus_tag="IYF54_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9832840.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            5074..>5698
                     /locus_tag="IYF54_002575"
     CDS             5074..>5698
                     /locus_tag="IYF54_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ9832841.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1719 a          965 c         1287 g         1727 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
     1081 cagctacccc agtttctata atttattctt tattttatag attgataata gtataatggc
     1141 ggtatagcca agtggtaagg cagaggtctg caaaaccttc atcaccggtt caaatccggt
     1201 taccgcctta gtactaataa atcaacttgc cggcgtggcg gaattggcag acgcgctgga
     1261 ctcaaaatcc agtgcccgtt gagggcgtgc cggttcgacc ccggccgccg gtattgacta
     1321 agatgtcttc atcttagttt tttattgaga aattaaataa catcttttat gatggcgaat
     1381 gtggcgaagt ggttaacgca ccggattgtg gctccggcat tcgtgggttc gattcccatc
     1441 attcgccctt tattcttggg gtatagccaa gcggtaaggc aagggacttt gactccctca
     1501 tgcgttggtt cgaatccagc taccccagtt attattttta aatttgccgg cgtggcggaa
     1561 ttggcagacg cgctggactc aaaatccagt gtccgttgag gacgtgccgg ttcgaccccg
     1621 gccgccggta taattatcaa tatagcaact ttcttataga gagttgctat attttttttg
     1681 tttttatgta attacctcca aatcaattcg atacaagtat tatcataaaa cgagtaaaaa
     1741 agatattgat aacgctttta ttatgtgtta aactattttt gtaaattatg tagggtgcta
     1801 ctattcaatt aggcacgacc taagaaaggg ttctttctta ggtcgttttt tgtctttaaa
     1861 aattgtttgg agacaaaaat atgtatgaag gagtctgcaa gttgttttgt tttatcatat
     1921 agaggaggtg aattaacggt gtatgtgatg aaaaagctga atcataatgc tgtattgatc
     1981 gatgacgatg gaatagaaaa aattgcgatg gggaaaggga ttggcttttc tgctaaagtg
     2041 agtaagccat ttgatgttga ggatgctgac aaattatttg tattagattc taaagaaaag
     2101 atcaaacgat tcagtgagat agtctcgcag attccaatcg aatttatgga ttttgcggaa
     2161 gaagtgattg aactgataac agctaggatc tcctctccat tagattcaaa tatctatatt
     2221 acattgacag atcatattta ttttgctatt caacgaaaaa gagaagatag agatgtcaca
     2281 gcaattatgt tgccagaaat gagattcctt tatccaaatg aatatgatat agcagtagaa
     2341 gtggtcgagc gaatcaatca tcgatatcaa accgagttag ggtcaaatga agtaggattc
     2401 atcactatgc atatcattaa tgctgagcta ggagagccta atagtttaaa gagcttaaaa
     2461 atagtagaaa tgacgaccac gattttgtcc catctagaaa aatcctattc aaacgtttat
     2521 gataaagaat cactgactta tcatcgctta atggttcata tcaaattcct ggttaaacgc
     2581 ttgatttatc aggaagaaac gccagtagaa aatattggtt tttttactga aacatttaaa
     2641 catagcaaag cgtattgtac agcactaaag attaaagata tcatcgaaca gcaatttcaa
     2701 cttgccattc aagaaaatga aatcatctat ttggcaattc atctagcaag aatctattaa
     2761 aataagggcg taactatcta taggcgcaac caaatgacac atagtaaatg gactatgatt
     2821 atttggttgc gcctatttta gtagaaaaaa gaaggagaat gaaatgggaa aagtgatttt
     2881 taattcggat gattttggct atagccatgg tgtgaattat gggattatgg atgcttatca
     2941 gcgaggaatt ttgacatcaa ctacattgat ggcaaatatg ccgggtttcg agcatgctgt
     3001 gaaactaaga aaggaaatgc ctaggctagg tgtaggagtc catttaacat tgacgtgtgg
     3061 caaaccactc ctcaaaactg tcgatactct tatcgaaagc ggagagttca gatccttatc
     3121 ttattatcag catccattta acattgacaa agatcaactg tatcaggaat ggaatgctca
     3181 gattcaaaaa atttatcatg cggggatcat accaactcat ttagacagtc atcatcatac
     3241 tcatactttg gatgaaaatc aggaggtagt tgttacatta gcaaaaaaat atgatttgcc
     3301 ggttcggagc aactttgaac gaaaagatga agttcgtcat gtcgattatt ttgagccttg
     3361 tttcgatgtc gtaggagaaa ctgatgaaaa aaaacgacaa atcaatacga gtgtggaaga
     3421 atatttggca agtttactca tgatattgag agaagataaa acgatcgaag tcatgtgtca
     3481 cgcagcgtat ttggatcagg aattgttaaa tggttcaagt tttgtgtatc ctcgtaccca
     3541 ccaagtcgaa tttttaattc attcagcatt tgctcaaaaa gtgaaaaatg atcctttgat
     3601 tgagttagta acatatgagg atatttaata gaggaggaat aaaaatgaat gttaaatcaa
     3661 atgtgcagaa gcttggaaga gcgatgctac ttccagttgc agcgatgcca ttagcgggat
     3721 tgatcatgcg tttatcagct gacgatatgc tgaacatccc agtaattggt gcagctggaa
     3781 atgccgtttt tggaaacttg gatatcttat ttgctatagg agttgcaatc ggttttgtca
     3841 aaaataagga caaaggaata cctgcactta ctggtttttt agcgattgct accttgaaaa
     3901 aaggcttaga aattatggac ccagaagtga atatggggat ttttggtgga attatttccg
     3961 gtttagtagc ggcatggacg tataatcgct ttaaagaaca aaaattacca gtagtttttt
     4021 cttattttgc gggcgaaaaa ttcccattga ctatggtgat gattctacaa acaatcgcag
     4081 ctgttttttt gggctttatc tggccgctca tccaaattgg gattgataat tttgcaaaac
     4141 tgctcgttga tatgggagca ttaggtgtgg ggatatttat gtttttgaat cgtttgctga
     4201 tcccctttgg actgcaccat gtgttgaata cgtatattta ttacgattta ggaagctata
     4261 catctcctag caaggaagtt ttcagaggag agatgacacg ctttatcaac ggagatcctc
     4321 aggctggact gtttttatca ggtttttttg tagtaatgat gtttggtata ccagctatat
     4381 gtctagctat ctataaagca gcttataaag aaaataaaga gatggtcaaa ggaatcatgg
     4441 gaagcggtgc aggaacttca tttattgcaa atatcactga gcctgttgaa tttagtttca
     4501 tgtttctttc tcctatgttg tacgtgatcc atgcactttt tgccggttta gcagggattg
     4561 tctgttactt attcaatata cgaattggtt ttacattcgg agcatgtatt gttgattatc
     4621 tcatcaattt tcgaatcgct accaatgcta ttttgattct tccaatcgga attttctttt
     4681 ttgccttgta ttacgttact ttttattatt tgatcaataa gagaaacatt cagacattag
     4741 gacgtgaagc taaggcagag tttggcaatg aagtaacctt agaagaaaca gagttagggt
     4801 tagcaagtaa aaattatgaa tatatggcaa caaaaatgct acaagcgttt ggaggaaaag
     4861 ccaatatcct ggatgtctat agttgtaata cacgcctacg ggtagaggta gtggatccca
     4921 ccatggtcga ggaacagcga atcaagcaat tagggataag cgggatcatt aaaccaactg
     4981 aaaaaaatta tcaaattatc atcggcttag aagtgactta tgttatggca gaattcaata
     5041 aattattaga agaatagaaa ggaaaagatg atgatgaaaa aacagattat cactatagct
     5101 ggaggcggga gtacgtatac accaggaatc gttcaagcca ttttgaacaa tgaaaaacgg
     5161 ttgccattat ctgaaatacg tttatatgat attgatgaag aaagaaatat ggatatgtac
     5221 ttgattgtta aatttatgct gaaaagaaaa ggtttttcaa atattcgcat tcgtgcaacg
     5281 gatgatccaa agcttgcttt tacaggatgc gattttgttt tctcacaaat tcgtgttgga
     5341 ggattggaga tgcgagaaaa ggacgaaaag attccactta aacatggatt agtaggacaa
     5401 gaaacgtgtg gactcggagg atttgcttat gggatgcgct caatcaaagg tttattggaa
     5461 attgtcgatc atatacaaga ctatgccccc aaagcatgga ttttgaatta tacaaatcca
     5521 gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga atgtaaaaat gattaatgcc
     5581 tgtgatatga ctatttcaat tgaagaaacg attgcagtga attacggtta tgatcgtaag
     5641 aattggatcg ttacgtatta tggtttaaac cactttggct ggtatacatc gatttatg
//
LOCUS       DACIWG010000101         5667 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.107, whole genome shotgun sequence.
ACCESSION   DACIWG010000101 DACIWG010000000
VERSION     DACIWG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.107"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..142)
                     /locus_tag="IYF54_002576"
     CDS             complement(<1..142)
                     /locus_tag="IYF54_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9832842.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IYF54_002577"
     CDS             complement(183..437)
                     /locus_tag="IYF54_002577"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9832843.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IYF54_002578"
     CDS             complement(548..802)
                     /locus_tag="IYF54_002578"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ9832844.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IYF54_002579"
     CDS             complement(995..1270)
                     /locus_tag="IYF54_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ9832845.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IYF54_002580"
     CDS             complement(1242..2195)
                     /locus_tag="IYF54_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9832846.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IYF54_002581"
     CDS             2807..4300
                     /locus_tag="IYF54_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ9832847.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IYF54_002582"
     CDS             4435..4731
                     /locus_tag="IYF54_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ9832848.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5624)
                     /locus_tag="IYF54_002583"
     CDS             complement(4755..5624)
                     /locus_tag="IYF54_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9832849.1"
                     /translation="MNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPE
                     EQSEYIQRKVVQGWTPDVIVGRAAFPIRCSARTIYRMFKKGLFNPSDLPMKGKRKPNG
                     HQERRGKQAFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIIT
                     LKPCGRQAIDIENKLNHWFESVPKNLFKSITFDCGKEFSNWKQISNANDIAIYFADPG
                     TPSQRGLNENSNGLLRRDGLLKSMDFNSVDESFIQSVASKRNNIPRKSLNYRTPLEVF
                     LSYVSIDDLSNLI"
BASE COUNT         1810 a          969 c          984 g         1904 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagcaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataa
//
LOCUS       DACIWG010000102         5128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.108, whole genome shotgun sequence.
ACCESSION   DACIWG010000102 DACIWG010000000
VERSION     DACIWG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.108"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..708
                     /locus_tag="IYF54_002584"
     CDS             <1..708
                     /locus_tag="IYF54_002584"
                     /inference="COORDINATES: protein motif:HMM:NF025056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ9832850.1"
                     /translation="KKLENNSKEDLFTSKNYVQWMTDVNYIFKTDLKNSIFKDEFEKA
                     YFYKNSRLKNSLYEIISILELIMSKSEGEITSMKEKIFISHSSKDKELAKEFVNFMKT
                     SFEINNRKIFCTSLNGSLGVGDNFITKIKKEIAESAIIIFLITPNYLSSKFCLCELGA
                     AWALDQNIFPILCESASIDDLNATPLLGTQCVKLDDEENMIDLAQMIVSTDLADSQSL
                     LDYKGNLKNFLKLIDAK"
     gene            754..1896
                     /locus_tag="IYF54_002585"
     CDS             754..1896
                     /locus_tag="IYF54_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9832851.1"
                     /translation="MFFENKLWYIVHEGDVFMNSDLFKIIEGGIEKDTQKVINYSKKL
                     AEQFYLEGKTSTGKKIERIIAENNTRVSSLDSLTSRPTDRDTKVDLVDVWIPFETGDT
                     LYFDSIIEKEIKNFTLTYYNRNRLIDHGIESSNNLLLYGPPGTGKSSLAKYIALQLKL
                     PLITVRMDGVISSLLGSTAKNIRKVFDYASKQPCILFLDEFDVIAKIRDDQSEIGELK
                     RVVNSLIQNIDSFSEDSILIAATNHSHLLDNAVWRRFDKVLFIRIPEYNTRKDIIVEF
                     SNRIENNFCNDSKKLDVLTSLTENFSPSQIKTTIGNSIKKTIVSGDELLKFSEVVYAI
                     FLEINQEDAVNDVDIIKFLHSNNISQREIAEQFSFSLRKVKDCLSN"
     gene            1911..4256
                     /locus_tag="IYF54_002586"
     CDS             1911..4256
                     /locus_tag="IYF54_002586"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HAQ9832852.1"
                     /translation="MDSKKSRLISFKQRDQDNLKVGPSPQTQVPTWVLSGADLDRKTK
                     FLIEKLDVIENNFPRFSTQELPDVLSVSFIEDAKAKTHQRKILDLMGSELNGRQIGMS
                     DDNSILLEFLSKDGIAKIKENIDSDQEKYKVSISAIEDFTPYKAKKNLNEKNKNNYLL
                     EFFDYSDKKMNENTFKYVEHLLKKNNIKFEEKIYNEDILLQIDNSSIKDALNLVENDS
                     IPIKSINPIAKTEVSPFRNVLAEAEEESMWVSYDEEKEYPIIGLLDSGVDMNSQTSQW
                     VVKGSGCNIPDEELDTSHGTFIATLLIHGNRYSNYTDCSVDGCRIVDVPIVPRYSIPE
                     NELINNIRDAISNNPEVKIWNLSVSLVEQIDQNSFSTFAIALDEIQDEFKVLICKSAG
                     NDRSFYAGSSAGIISKGAESLRSLTVGSILRNSDNFGFCKKGFPAPYSRIGRGPANII
                     KPEVVHFGGDVFALNAAPTSVEDYQSQGETSYLNAVKRIEAGTSFSTPKIAKSLSEIN
                     NSLIDSDLLLSKTLLIHSSTYFNNPDMKIEDKIKALGFGYPKKSSDILVEDTEYRTTM
                     ILKGELRKSNFIDIMSFPFPKNLLRDGHYYGKIKITLCYDNILDKRMASEYSQSNITL
                     RFGTFEKIEEVRPFANQKVPTNPFKRIGSKNVLLENRYSVNSMRNNTEYASERKLIKG
                     DFKYHSTKQYSIDLSETKPSKLSDLESDKSWFLYMSPEFRNPALDKYSSDQLKMNFCC
                     IITIEDPFKENNIYEGVIKSLEENNFEYNNIEINQDLSINL"
     gene            4760..>5128
                     /locus_tag="IYF54_002587"
     CDS             4760..>5128
                     /locus_tag="IYF54_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAQ9832853.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEV"
BASE COUNT         1949 a          648 c          880 g         1651 t
ORIGIN      
        1 aaaaaacttg aaaataattc aaaagaagat ctatttacaa gcaaaaatta cgtccaatgg
       61 atgactgacg taaattatat ttttaaaacc gatttaaaga atagtatttt taaagatgaa
      121 ttcgaaaaag cttattttta caaaaatagt agattgaaaa atagtttata tgaaattata
      181 tctattttag aactgattat gagtaagtca gaaggagaaa taactagtat gaaggaaaaa
      241 atttttataa gccatagtag taaagataag gagttagcta aagaatttgt aaatttcatg
      301 aaaacatctt ttgaaattaa taatagaaaa atattttgta cctcattgaa tgggagtcta
      361 ggagtaggag ataattttat cacaaaaata aaaaaagaaa ttgctgaatc agcgattatt
      421 atttttttaa taactcctaa ttatctgagt agtaaatttt gcttatgtga gctaggagct
      481 gcatgggctc tcgatcaaaa tatttttcct attttgtgcg aatcagcaag cattgatgat
      541 ttaaatgcaa cacctttatt aggtacacaa tgtgtaaaat tagatgatga agaaaatatg
      601 atagatttag ctcaaatgat tgtatctact gatttagcgg atagtcaatc tctattagat
      661 tacaaaggta atctgaaaaa ctttttaaaa ctaattgatg caaagtaaag cagtttttta
      721 tggaacgaaa aagcaatata tgggaacaaa tatatgtttt tcgagaacaa attatggtat
      781 attgttcacg aaggggatgt atttatgaat tctgatcttt ttaaaatcat tgaaggcggt
      841 attgaaaaag atactcaaaa ggttataaat tactcaaaga aactagcgga acagttttat
      901 ctagaaggga aaacatctac tggtaagaaa atagagagaa taattgcaga gaacaatacg
      961 cgcgtttcct cgttagattc actaacttct agaccaacag atcgagatac aaaagtagat
     1021 cttgtcgatg tatggattcc ttttgaaact ggcgacacct tgtattttga tagtattata
     1081 gaaaaagaaa taaaaaattt tactttaaca tattacaaca gaaataggct aattgatcat
     1141 ggaattgaaa gttcaaataa tttattgttg tatggaccac caggtactgg taaatcttct
     1201 ttagcgaaat atattgcttt acaattaaag cttccactta ttactgtaag gatggatggg
     1261 gttatttctt cacttttagg aagtacagca aaaaatatca gaaaggtatt tgactatgct
     1321 tctaaacaac cgtgcatttt attcttagat gaatttgatg tcatagctaa aatacgtgat
     1381 gatcaaagtg aaattggcga gcttaaaaga gtggtaaata gcttaattca aaatattgat
     1441 tccttttcag aagatagtat attgattgca gctaccaatc attcgcattt gttagataat
     1501 gcagtttgga gacgttttga taaagtattg tttattagaa ttcctgaata taatacacga
     1561 aaagatataa tagtagagtt ttcaaataga attgaaaaca atttttgcaa cgattcgaaa
     1621 aaattagatg tactaactag tcttacagaa aatttttctc catcacaaat aaaaactaca
     1681 ataggaaatt caataaaaaa aactatagtt agcggagatg aattgttgaa atttagtgaa
     1741 gtagtgtatg caatattttt ggaaattaat caggaagatg ctgtgaatga tgttgatata
     1801 attaaatttt tacattctaa taacatttca caaagagaaa ttgcagaaca gtttagtttt
     1861 tcactgagaa aggtcaaaga ctgtttatca aattgaggac gtgatactat atggattcga
     1921 aaaagtccag attaatcagc tttaaacaaa gagatcaaga taatttgaaa gttggaccat
     1981 ctccgcaaac tcaagttcct acctgggttt tgagtggtgc agatctagat agaaaaacta
     2041 aatttctaat agaaaaatta gacgtgatag aaaataattt ccctcgattt tcgactcaag
     2101 aactacctga cgtactatca gttagcttta tagaagatgc aaaagcaaaa acgcatcaaa
     2161 ggaaaatatt agatctcatg ggctctgaat taaatggtcg tcaaataggt atgtcggatg
     2221 ataacagcat attactagag tttttatcaa aagatggcat agctaaaatc aaagaaaata
     2281 ttgatagtga tcaagaaaaa tataaagttt ctatttctgc tattgaggat tttactcctt
     2341 acaaggcaaa gaaaaatttg aatgaaaaaa acaaaaataa ttatctcttg gaattttttg
     2401 actactcaga taaaaaaatg aatgaaaata cttttaaata tgttgagcat ctgttaaaga
     2461 agaataatat aaaatttgaa gaaaaaattt acaacgaaga cattcttttg caaattgata
     2521 attcttctat aaaagatgcg ttaaaccttg ttgagaatga ttcaattcct ataaaaagta
     2581 taaaccctat cgcaaagacg gaagtgtctc cctttcgaaa cgttttagct gaggcggaag
     2641 aagaaagtat gtgggtatca tatgatgaag aaaaggaata tccgataata ggtcttttag
     2701 attctggtgt tgacatgaat tcccaaacta gccaatgggt tgttaaggga agtggctgta
     2761 atattcctga tgaggagctt gatactagtc atggtacttt tattgccaca ttactaattc
     2821 atggaaatcg atatagtaac tacactgatt gttctgtaga tggctgcaga atagttgatg
     2881 tgccaattgt gccaagatat agtattcctg aaaatgaatt aattaacaat attagagatg
     2941 ctatttctaa taatcctgaa gtaaaaatat ggaatctatc tgttagttta gtagagcaaa
     3001 ttgatcagaa tagtttttct acatttgcaa ttgcattgga tgaaattcaa gatgaattta
     3061 aagtattgat atgtaaatct gctggaaatg ataggtcatt ttacgctgga agctctgcag
     3121 gaataattag caagggtgct gagtcacttc gttcattaac agtaggatca atactgagaa
     3181 atagcgataa ttttggcttt tgcaaaaaag ggtttcctgc tccatattct cgcattggga
     3241 ggggacctgc aaatattata aaacccgagg tagttcattt tggaggagat gtatttgcac
     3301 taaatgctgc tcctacaagt gtagaagatt atcaaagcca aggagaaaca tcatatttaa
     3361 atgccgtaaa aagaattgaa gccggcacga gtttttctac tcctaaaatt gcaaaatctc
     3421 tatctgaaat aaacaatagt ttaattgatt ctgatttact tttatctaaa accttattga
     3481 ttcattcttc aacttatttt aataatccgg atatgaaaat tgaggataaa attaaagcgc
     3541 taggttttgg ttatccaaaa aaatcttcag atattttagt tgaagatact gaatatagaa
     3601 cgacaatgat tttgaagggt gaattaagaa aaagcaattt tatcgatata atgtcttttc
     3661 cttttccaaa aaacttactt agagatggac attattatgg taaaataaaa atcactcttt
     3721 gttatgataa tatattagat aagcgaatgg caagcgaata ttctcagtca aatataacac
     3781 ttaggtttgg aacttttgaa aaaattgaag aagtaagacc ttttgctaat caaaaagttc
     3841 ctactaaccc cttcaaaaga atcggttcta aaaatgttct tttagaaaac agatacagtg
     3901 ttaattctat gagaaacaat actgagtatg cttcggaacg aaaactgata aaaggtgact
     3961 ttaaatacca tagtaccaaa cagtactcaa ttgatttgtc tgaaactaaa ccatcgaagt
     4021 tgagtgattt agaaagtgat aaatcatggt ttctatatat gtcaccagag tttagaaacc
     4081 cggcattgga taagtatagt tctgaccagt taaaaatgaa tttctgctgt ataataacta
     4141 ttgaagatcc atttaaagaa aataatattt atgaaggcgt tataaagtct ttagaagaaa
     4201 ataattttga atataacaat atagaaatta atcaagacct gtcaataaac ctgtaaaaaa
     4261 actagaccta tttttaggtc tagttttttt acaggtttac ataagataca ttatgcgaag
     4321 ttcattattt taaaagctta aataataaac ttttttataa taccaattta tttatgtaaa
     4381 ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc tccaaaacta
     4441 atgactatct attttcaaag aagctagaat gattatctga tcaaatgata atttatattt
     4501 aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg tacacgataa
     4561 agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa aatcactaaa
     4621 ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat tttgatgaat
     4681 tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta ttaccaaata
     4741 gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg gtagaaatcc
     4801 caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg ctagagaact
     4861 agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg atttaagttt
     4921 aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt atgttctaat
     4981 tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg ttgggtatga
     5041 aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat ttatcccaat
     5101 aattaaagaa ggacgtgcaa gcgaagta
//
LOCUS       DACIWG010000103         4809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.110, whole genome shotgun sequence.
ACCESSION   DACIWG010000103 DACIWG010000000
VERSION     DACIWG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.110"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IYF54_002588"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IYF54_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ9832854.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IYF54_002589"
     CDS             complement(3250..3768)
                     /locus_tag="IYF54_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ9832855.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1578 a          787 c          732 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagggactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccacttagc
//
LOCUS       DACIWG010000104         4806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.111, whole genome shotgun sequence.
ACCESSION   DACIWG010000104 DACIWG010000000
VERSION     DACIWG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.111"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYF54_002590"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYF54_002590"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ9832856.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="IYF54_002591"
     CDS             complement(1276..2112)
                     /locus_tag="IYF54_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832857.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="IYF54_002592"
     CDS             complement(2296..3513)
                     /locus_tag="IYF54_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ9832858.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3896..3979
                     /locus_tag="IYF54_002593"
     tRNA            3896..3979
                     /locus_tag="IYF54_002593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4806)
                     /locus_tag="IYF54_002594"
     CDS             complement(4219..>4806)
                     /locus_tag="IYF54_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832859.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1460 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaaa
//
LOCUS       DACIWG010000105         4790 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.112, whole genome shotgun sequence.
ACCESSION   DACIWG010000105 DACIWG010000000
VERSION     DACIWG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.112"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(74..1072)
                     /locus_tag="IYF54_002595"
     CDS             complement(74..1072)
                     /locus_tag="IYF54_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ9832860.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="IYF54_002596"
     CDS             complement(1147..1443)
                     /locus_tag="IYF54_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ9832861.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="IYF54_002597"
     CDS             complement(1436..1729)
                     /locus_tag="IYF54_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832862.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1875..2426)
                     /locus_tag="IYF54_002598"
     CDS             complement(1875..2426)
                     /locus_tag="IYF54_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ9832863.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2600..2899)
                     /locus_tag="IYF54_002599"
     CDS             complement(2600..2899)
                     /locus_tag="IYF54_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ9832864.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2926..3192)
                     /locus_tag="IYF54_002600"
     CDS             complement(2926..3192)
                     /locus_tag="IYF54_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ9832865.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3479..4222
                     /locus_tag="IYF54_002601"
     CDS             3479..4222
                     /locus_tag="IYF54_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832866.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4259..4756)
                     /locus_tag="IYF54_002602"
     CDS             complement(4259..4756)
                     /locus_tag="IYF54_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832867.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1754 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacacacccc
     1741 ctgttatttt ttacataatg aaaatagcat attactgggt acattgataa ttcatataac
     1801 atgtatcatc acatgtattt ttaaagtcaa aaagcatgta aaactactta taagtaacat
     1861 gagaaaataa aatgttattt ttcttgaaat aataattgac tatctgccaa ttgccattca
     1921 ttacgaacat gaatacttag tgggtgggat aacttacctt ctgcatttat caatactttg
     1981 gtcatcttct tttgtttcgc actcaattga tcgtaaatag tctttgcttg ttcataagcc
     2041 acttgactcg tcgctgtaat cgaaccacaa gcaaaaataa tggtattgat tttcttttct
     2101 gtaaaaacag tcgttaatat ttccaaagat tgttcatcat ttcctcgaag aaatacccga
     2161 ggacttttcg cttttgtgta cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat
     2221 tgtcggatat tcttttcgat taataacata ctcaaatcat tcgtaaatgg ttctgtatct
     2281 gttggacgaa tagtaatgac tactgctaat ttatcttccg cttcttttcc tttgatcgcc
     2341 cattgtcgtt tgaaaacata gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt
     2401 gtggtcacta attctttttc aatcatctct ttttctccta tgctattttt tgttcaagca
     2461 cttcaattac cattgccgtt ataagtgtca atactagttg aaaatatttc atattctttc
     2521 tccttttttc ttcagaattt gatgaatcgc ggtaaggata tcttctaaac atttcgcaat
     2581 cattctcaaa taatcgatct tattcatccg ttcctccaat ctgactccaa accaatcgtc
     2641 cttctaccag taacccacga acaaattctc gctggctggt aatatctgta atgaaataaa
     2701 ctacatagtc ttttccatct tctggtgcgc aataaatata aagatgtgtc atcactaaac
     2761 gtggtagatg aacatcttca ggaagcatac ccatttcaat ttcctctttt tccatcgtaa
     2821 cggattcata gccccactct tcagaagcag tgaccagctg tgcacacttt tccagtactt
     2881 cgatagccgt ttctaacaag tcctttcgtt cctttctttt agatactagt attcttctgg
     2941 taataacatc gtttgatact cacccgaatc aatggcccat attttttgag acactgattt
     3001 caaacgaagc ttaggtgaaa tcagatactc ttttctttct tccggttctt cttgacgatg
     3061 aaccaacttt tgatttcctt tttccgtaga aaattcaaaa atttgaagat agtctaaagg
     3121 aaattgcttt tgaacgtttt gatcgattag attccaacat aaagcttgta attccaccgg
     3181 caaactgtcc attactccct ttgtgacata acgttatcct ttattttgaa acatttcttt
     3241 tccctccttt gccataaaaa tagcccaatc ttctattaaa gaagattggg cttcatggat
     3301 ataatatata tttgtaaata aagttaattc agttataatt atcaaaacta cacaattatt
     3361 aaagtttttc atatctcttt gtttagtaag tttttctaaa tcttaacccg tggtgctagt
     3421 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcatat
     3481 agtagtctca aaactcccaa gaaatgagga ctatttaaca aactacgatg gttacgtcgt
     3541 gacaactgaa cattttgact tagccaatcc tgttactaaa gtcgatgata tccccgatta
     3601 tgaaatgtac agtcaaacaa tcgatagctt aaataagcgt tttggcaatc gtgttttaaa
     3661 aggcatcaaa atcggttaca tcgcttctga aaaggatcgg atcatcgact atttagccga
     3721 taaagattac gatttaaaat tactcagtgt gcatcataat gggcaatttg attatttaga
     3781 tgacgaagtc aaagatatgg atcctgctat tgtgattcct cagtattttg cacaactttc
     3841 agaagcacta gttgttatag aagcagatgt ctttgctcat tttgactatg gttttcgggt
     3901 atttggtcta tccgttgctg aatttaaaca atatgaagcg cagtttttac cgattctaga
     3961 tcaagtaatc aaaaataaat tggcattcga attaaatgct aagagcgctt acttgtatga
     4021 caatttagcg ttatatgaat atgtcatcga tttatatctt tcacgtggtg gtaccttgtt
     4081 tagtgttgga tcagatgggc attaccttga acatttccgt ttccatttcg atgatctttt
     4141 tgctctgtta aaagcaaatg gtgtgacgga attagcgatt tatcaaaaag gaaagcgaat
     4201 tatggttcca ttaccagtct aaaaaaacgt cctcagcatc aaaagtgagg acgttttttt
     4261 agatttcttt ttctaaaatc ggacaaatac gatcttgttt tcctaaagga aaatgtttga
     4321 tcccgatttt tttaaatcca tgacgttcat aaaaacgaat acctgcatga tttttttcat
     4381 aaacagtcaa acgtaaataa cgtttttgtt tcgttttagc ataacgattc gcttcatcga
     4441 gtaaccgttg ccctaaccct ttgttttgat aatctttgag cagatagagc cgttgaatct
     4501 ctatagcatc tttttcatca ttgactttca tataaccaat gagtatttga tgatccttaa
     4561 taccaataaa tttactggtt gactgtgcca attctactgc caatatttca gttgtcaatt
     4621 gtgtctgtaa atatccttgc atatcaagtg gatctgcgcc atcgataaac gattcataaa
     4681 aactgacaac acttacaact tgtaaagcat ctagatcatc ataatctaaa tacgctagtg
     4741 tgatttgatt gttcatctaa ctcctccttt tgacatagta tactataaat
//
LOCUS       DACIWG010000106         4465 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.113, whole genome shotgun sequence.
ACCESSION   DACIWG010000106 DACIWG010000000
VERSION     DACIWG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.113"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            574..1008
                     /locus_tag="IYF54_002603"
     CDS             574..1008
                     /locus_tag="IYF54_002603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832868.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IYF54_002604"
     CDS             2298..3221
                     /locus_tag="IYF54_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ9832869.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IYF54_002605"
     CDS             3237..3776
                     /locus_tag="IYF54_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ9832870.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACIWG010000107         4305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.114, whole genome shotgun sequence.
ACCESSION   DACIWG010000107 DACIWG010000000
VERSION     DACIWG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.114"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            148..1137
                     /locus_tag="IYF54_002606"
     CDS             148..1137
                     /locus_tag="IYF54_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ9832871.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IYF54_002607"
     CDS             1134..1553
                     /locus_tag="IYF54_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832872.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IYF54_002608"
     CDS             1562..1870
                     /locus_tag="IYF54_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832873.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IYF54_002609"
     CDS             1874..2236
                     /locus_tag="IYF54_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832874.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IYF54_002610"
     CDS             2458..3864
                     /locus_tag="IYF54_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ9832875.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACIWG010000108         4234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.115, whole genome shotgun sequence.
ACCESSION   DACIWG010000108 DACIWG010000000
VERSION     DACIWG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.115"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(37..1257)
                     /locus_tag="IYF54_002611"
     CDS             complement(37..1257)
                     /locus_tag="IYF54_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9832876.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IYF54_002612"
     CDS             complement(1277..1882)
                     /locus_tag="IYF54_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ9832877.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IYF54_002613"
     CDS             complement(1931..2863)
                     /locus_tag="IYF54_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ9832878.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IYF54_002614"
     CDS             complement(3284..4144)
                     /locus_tag="IYF54_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832879.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1203 a          853 c          695 g         1483 t
ORIGIN      
        1 tttattcttt atttaaagct ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gata
//
LOCUS       DACIWG010000109         3680 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.117, whole genome shotgun sequence.
ACCESSION   DACIWG010000109 DACIWG010000000
VERSION     DACIWG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.117"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..276
                     /locus_tag="IYF54_002615"
     CDS             <1..276
                     /locus_tag="IYF54_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ9832880.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IYF54_002616"
     CDS             419..640
                     /locus_tag="IYF54_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832881.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IYF54_002617"
     CDS             656..1498
                     /locus_tag="IYF54_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832882.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IYF54_002618"
     CDS             1561..3363
                     /locus_tag="IYF54_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ9832883.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3680)
                     /locus_tag="IYF54_002619"
     CDS             complement(3422..>3680)
                     /locus_tag="IYF54_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ9832884.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          521 c          750 g         1098 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcctatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaatggatat agtaatgccc
//
LOCUS       DACIWG010000110         3641 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.118, whole genome shotgun sequence.
ACCESSION   DACIWG010000110 DACIWG010000000
VERSION     DACIWG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.118"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            77..337
                     /locus_tag="IYF54_002620"
                     /pseudo
     CDS             77..337
                     /locus_tag="IYF54_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            482..1336
                     /locus_tag="IYF54_002621"
     CDS             482..1336
                     /locus_tag="IYF54_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9832885.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            1407..1622
                     /locus_tag="IYF54_002622"
     CDS             1407..1622
                     /locus_tag="IYF54_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAQ9832886.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            1639..1911
                     /locus_tag="IYF54_002623"
     CDS             1639..1911
                     /locus_tag="IYF54_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ9832887.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            1913..2776
                     /locus_tag="IYF54_002624"
     CDS             1913..2776
                     /locus_tag="IYF54_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ9832888.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            3320..3431
                     /locus_tag="IYF54_002625"
                     /pseudo
     CDS             3320..3431
                     /locus_tag="IYF54_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            3452..3535
                     /locus_tag="IYF54_002626"
     CDS             3452..3535
                     /locus_tag="IYF54_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ9832889.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         1386 a          547 c          654 g         1054 t
ORIGIN      
        1 gctaaagagg tccctagcgc ctacgaggaa tttgtatcga taaggggtac aaattcccac
       61 taagcgctcg ggaccccttg taggaaaatg tcctaagtgt ggcaacaata ttgtattaaa
      121 aaaatcgttt tatggttgtt caaattatcc tgaatgtaag tttactttag ctgaacattt
      181 tagaaagaaa aaactaacca aaacgaatgt aaaagaatta ctggagggaa aagaaaccct
      241 ggtaaaagga atcaaaaaca aagagaaaaa gccctacaat gccgttgtaa aaattgggga
      301 aaagggatat attgatttta tctctttttc aaaataaaca taaaagccct ttaaagaggg
      361 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
      421 ttgtgatttg tgattcctaa tgatacaata ttactatata aaaaaagaat ggggcgtagt
      481 tatggagaag gaagaactaa aaatacttga agaattaaga cgtattttaa acagtaaaaa
      541 tgaagcaatt gttatcttaa acaattattt taaaggtggt gttggaaagt caaaattatc
      601 gactatgttt gcttatttga cagacaaatt taatttaaaa gttttaatga tcgataagga
      661 cttacaagca acattgacaa aagacttagc aaaaacattt aaggtagaat tgccacgtgt
      721 caatttttat gaaggcttga aaaatggaaa cttggcttct tctattattc atttgactga
      781 taatttagac ttgatccctg gcacgtttga tttgatgtta ctgccaaaat taactcgctc
      841 atggacgttt gaaaacgaaa gtagattgct tgctactctt ttagcacctt taaaaagtga
      901 ctatgatctc attattattg atactgtacc aacgccaagc gtttatacaa ataatgcaat
      961 cgtggcaagt gattacgtta tgatcccttt acaagcagaa gaagaaagta caaacaacat
     1021 tcaaaactat atttcctatt tgattgattt acaagaacag tttaaccctg gactagatat
     1081 gatcggtttt gttccttatt tagttgatac ggacagcgca acgataaaat caaacctgga
     1141 agaactgtac aaagaacata aagaggataa tttggttttc cgaaatatta tcaagcgaag
     1201 taataaagta agtacctggt ctaaaaacgg cattacagaa cacaaaggct atgacaaaaa
     1261 agttttatct atgtatgaga acgtattttt tgaaatgctt gagcgaatca ttcaattaga
     1321 aaacgaaaaa gaatagaatc acaaatcact tgtgatttgt gattgttgat gataaaataa
     1381 gaataagaag aaatagaaag aagtgagtga ttgtgggaaa tttaggcgca caaaaagcaa
     1441 aacgaaatga tacaccaatc agtgcaaaaa aagatataat gggagataag acggttcgtg
     1501 ttcgtgctga cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa
     1561 aagaagttat ggataaagcc ttagaagaat atattcggaa atatttacct gacaaacttt
     1621 aaaaggagtg aaaatgaaat ggcagttaca tatgaaaaaa catttgaaat tgaaatcatt
     1681 aatgagttat cggcaagtgt ttataatcga gtattaaact atgttttgaa ccatgaatta
     1741 aataaaaatg actctcaatt attggaagtc aatttattaa accaattaaa gcttgcaaaa
     1801 cgtgtaaatc tttttgatta ttctttagaa gaattacaag ccgttcatga gtattggcgg
     1861 tcaatgaatc gttactcaaa acaagttttg aataaagaga aagtggctta atatggcaaa
     1921 tatagtcaat tttactgaca aacaatttga gaatcgctta aatgataatt tagaagaatt
     1981 gattcaagga aaaaaagcgg ttgaatcgcc aaccgctttt ttacttggtg ggcaaccagg
     2041 gtcagggaaa accagtttgc gatcagcaat tcttgaagaa acacaaggga atgttattgt
     2101 cattgataat gataccttta aacaacagca ccctaatttt gatgaactag cgaaacttta
     2161 tgagaaagac gtagtaaaac acgtcacccc ttattctaat cgcatgacag aagcgatcat
     2221 aagccgtttg agcgatcaag gctataattt agtgattgaa ggtacaggac gaacaacaga
     2281 cgttcctatt caaaccgcaa caatgcttca agccaaaggt tatgaaacaa aaatgtatgt
     2341 tatggcagta cctaaaatca actcatattt aggaacaatt gaacgatatg aaactatgta
     2401 tgcagatgat ccaatgacag ccagggcaac accaaaacaa gcgcatgata ttgttgtcaa
     2461 aaacttaccg accaatttag aaacccttca taaaacgggc ttatttagcg atataaggct
     2521 ttacaataga gaaggagtga aactctattc aagattagaa acgccttcta ttagtccgaa
     2581 agagacctta gaaagagaat taaatcgtaa agtatcaggg aaagaaattc aaccgacttt
     2641 ggagagaata gagcaaaaaa tgcttcaaaa tcaacaccaa gaaacccctg aatttaaagc
     2701 aattcaacaa aaaatggaaa gcttgcagcc acctacacca ccaataccca aaacacctaa
     2761 acttccagga ctttaaacct taaaaaagga aaagagtagt taccaaaaaa cggtaactac
     2821 tcttttttta taaaaacatt tcctcatgtt ttaaaagctg atcgagaaat aaagcacaca
     2881 acggtattct ttcttttttc agttggtaca ttttgcagtc tgtaacgtgt catttactgt
     2941 ttgttacctt aacatatcca gtcgaaaaca caagggtata taaacgagca agctcgccct
     3001 ggttctgttg caaagttaga aaaatatagc taattggggt cccgagcgcc tacgaggaat
     3061 ttgtatcgat aagaaataga tttaaaaatt tcgctgttat tttgtacatt taacttgacg
     3121 gtgacatctc tctattgtga gttattagtg gtacagtttt caaccgtttt aatcataaaa
     3181 aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt attgcaggtg tatttcttat
     3241 ctatgggttt aacatggatt ttatcattaa aatcatgagt attgtccgag agtgattggt
     3301 cttgcgtatg gttaacccta aagttatgga aataagactt agaagcaaac ttaagagtgt
     3361 gttgatagtg cattatctta aaattttgta taataggaat tgaagttaaa ttagatgcta
     3421 aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc gtaatgtaga
     3481 taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata aataaatacg
     3541 ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa ctaaaattac
     3601 aaacaaatcg tttaacttct gtatttattt atagatgtaa t
//
LOCUS       DACIWG010000111         3638 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.119, whole genome shotgun sequence.
ACCESSION   DACIWG010000111 DACIWG010000000
VERSION     DACIWG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.119"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..330
                     /locus_tag="IYF54_002627"
     CDS             <1..330
                     /locus_tag="IYF54_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832890.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="IYF54_002628"
     CDS             complement(844..1011)
                     /locus_tag="IYF54_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9832891.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IYF54_002629"
     CDS             complement(1099..3039)
                     /gene="tet(S)"
                     /locus_tag="IYF54_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ9832892.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(3040..3126)
                     /locus_tag="IYF54_002630"
                     /pseudo
     CDS             complement(3040..3126)
                     /locus_tag="IYF54_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         1169 a          606 c          563 g         1300 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgtaagtt gattttatta aacatatgcc ttactttgtc
     1141 tattctatta tttggacgac gtggttggaa agcagactta atgttagtaa cctgataacc
     1201 ttttaactct gttaaacaga cactgcgtcc atttgtaaag aaagttaaac tgtttcgata
     1261 ctcttgaata caacgggctg gaatttcacc aatgagaatg acctcgttac cttttaactt
     1321 agtatttaaa atatttgcac aatatttgga agcatcatta tatgctctcg aaagatattc
     1381 ttgtggtaca taaatttcga agctaagata tggctctaat aactctgtac cactctttct
     1441 aaaagcctgc tctagtacaa taggcgcaag cattcggaaa tctgctggcg tactgacagg
     1501 gctgtaatat agaccatact taaaacagat cttacagtct gttaatttcc aaccgtacaa
     1561 tccttgttca cacccatagc gtataccttc cataactgca ttttgaaatg attgatttaa
     1621 ataacctaga gaaaccaggc tctcatactg aatgccacta cccaaaggaa gtggtgttac
     1681 agataaacca atagaagccc agaaaggatt tggaggtact tcgatatcaa tggtaaattc
     1741 agattttttt aatggtcttt ccatataaat gacagttggt tttcttgttt ctatctcaat
     1801 atgatatttt tcttgaatca gagtacaagt tacctccatt tggacctcac ctaaaaaaga
     1861 tagcacaatt tcgtgagtta ctgtatctac ataatattgt agaaggggat cactatcgga
     1921 tatttcaaaa agtgcatcta gtaacttttc tctttgtact gatttacatg gttcaattgt
     1981 tgtttgtaac ataggaagag gattctcaag aatttctcta tgtggtaatc tttttttatc
     2041 tccaagtacg ttatttagtt ttaaaagctc attttttaaa ataataatct caccaggctc
     2101 tgccttatct atctggcgta attctccatt tattgaagta tacatttctg taacttttat
     2161 tttttccttt tctgatatat tgactgagtc tcgcaaatgt agcgttccac tataaagacg
     2221 tacatagaca agccgttgac catcatctga atattctact ttaaaaacat ttccacaaag
     2281 tttatctgaa ttgagttgtg tgggtgaaaa taatttgcta gtaattacct ctataagttg
     2341 tttaattcca atattattct ttgcacttcc gtgataaaca ggatataagg agcaactttg
     2401 aattctttcg ttttcctcct tttcaagttc tgcaatattc aatgttttcc caatggtata
     2461 tttttctaat aaataatcat ttcccacaat tactgtctcc cattgttctg gttcagtata
     2521 atctattaca taaggtttca aatttagatt cacagtctgc ttaattatga tgtcgtcaga
     2581 aagtttgtcc ttaatatctt gataaacatc tggcaaattt attccatttt gatcaatttt
     2641 gttaataaaa aatattatag gtatgttcat ctttctaagt gcatggaata gtatacgagt
     2701 ttgtgactgt actccatctt ttgcagagat tagcaaaata gctccatcca aaacagataa
     2761 tgaacggtat acatctgcca aaaaatccat gtgtccagga gtatctacaa tatttacttt
     2821 aacattttcc cgttgaaaag atgttattgc ggtctgaata gtaatacctc tctggcgttc
     2881 caaaaacata gtatccgttt tcgttgtacc gctatctaca cttcctaact ctttaattgc
     2941 tccgctactg tatagtaagc tttctgtcaa agtagttttt cctgcatcaa catgtgctaa
     3001 gataccgata ttaataattt tcaattttat ttcctccatt caaaagctca aaaagcagaa
     3061 aaaatcccag tgataaatac tcttatcact gggattttaa tacaaaaata gctgtatact
     3121 acacactgat agccacagat gctttatgat aaaatgataa aagtattctt aaactgggca
     3181 caaaaaacca gtccttattt taaataagga tgcttaatta attattcccg ctatctaatt
     3241 gacagtttat ttaagaatac cttgccgcat ttttattatc tccttttgaa tgttatctta
     3301 attctatcat atgatagaat gcttgtaaag ccaaccatat tttttgaacg aaagggaaat
     3361 taatgagaaa ttaatggaag ttagtgtagt agttgaagat aactcttcag aatggctatc
     3421 aaaattcagc gatttaataa cgatttacaa gttcataagg gattctttat tcatccagct
     3481 attatattac ttttcaggtc aataggacaa ctgtccacaa cctttctaca aatatattac
     3541 gtataatttt tgaaatatat actgcttaac aaaatatttt gcagtggttc caatagtttt
     3601 gaattgttaa tattaagttt gacttggaaa atatcatt
//
LOCUS       DACIWG010000112         3484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.120, whole genome shotgun sequence.
ACCESSION   DACIWG010000112 DACIWG010000000
VERSION     DACIWG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.120"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(5..1495)
                     /locus_tag="IYF54_002631"
     CDS             complement(5..1495)
                     /locus_tag="IYF54_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ9832893.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(1890..2129)
                     /locus_tag="IYF54_002632"
     CDS             complement(1890..2129)
                     /locus_tag="IYF54_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9832894.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
     gene            complement(2491..3228)
                     /gene="erm(B)"
                     /locus_tag="IYF54_002633"
     CDS             complement(2491..3228)
                     /gene="erm(B)"
                     /locus_tag="IYF54_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9832895.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(3353..3436)
                     /locus_tag="IYF54_002634"
     CDS             complement(3353..3436)
                     /locus_tag="IYF54_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ9832896.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         1026 a          610 c          551 g         1297 t
ORIGIN      
        1 attttcagcc tgtatcatag ctaaacaaat cgagttgtgt gtccgttttt gggcgttctg
       61 ctagcttgtt taaagtctct tgaatgaatg tatgttctaa gtcaaaaaac tctgacagcg
      121 cctttatata gctttctttt tcttcttttt ttactttaat gatcgatagc aacaatgatt
      181 taacactagc aagttgaatg ccaccatttc ttcctggttt aatcttaaag aaaatttcct
      241 gattcgcctt cagtaccttc agcaatttat ctaatgtccg ctcaggaatg cctagcgctt
      301 ctctaatctc ttttttagtc gtcaccaaat aaggcttgta tacatcactt ttttcgctaa
      361 tataagccat taaatcttct ttccattctg acaaatgaac acgttgacgt tcacttcttt
      421 ttttcttgaa tttaaaccac ccttgacgga caaataaatc tttactggtt aaatcacttg
      481 atacccaagc tttgcaaaga atggtaatgt attccctatt agccccttga tagttttctg
      541 aataggcact tctaacaagt ttaatcactt ctttttcttc taagggttga tctaatcgat
      601 tattaaactc aaacatatta tattcgcacg tttcgattga atagcctgaa ctaaagtagg
      661 ctaaagagag ggtaaacatg acgttattac gccctattaa acccttttct cctgaaaatt
      721 tcgtttcgtg caataagaga ttaaaccagg gttcatctac ttgttttttg ccttctgtac
      781 cgcttaaaac cgttagactt gaacgagtaa agcccttatt atctgtttgt ttgaaagacc
      841 aatcttgcca ttctttgaaa gaataacggt aattgggatc aaaaaattct acattgtccg
      901 ttcttggtat acgagcaatc ccaaaatgat tgcacgttag atcaactggc aaagactttc
      961 caaaatattc tcggatattt tgcgagatta ttttggctgc tttgacagat ttaaattctg
     1021 attttgaagt cacataaact ggcgtttcta aaacaaaata tgcttgataa cctttatcag
     1081 atttgataat taacgtaggc ataaaaccta aatcaatagc tgttgttaaa atatcgcttg
     1141 ctgaaatagt ttcttttgcc gtgtgaatat caaaatcaat aaagaaggta ttgatttgtc
     1201 ttaaattgtt ttcagaatgt cctttcgtgt atgaacggtt ttcgtctgca tacgtaccat
     1261 aacgataaac gtttggtgtc caatgcgtaa atgtatcttg attttcgtga atcgcttctt
     1321 cggaagtcag aacaacgcca cgtccgccaa tcatgctttt ttttgagcga tacgcaaaaa
     1381 tagccccttt gcttttacct ggcttggtag tgattgagcg aattttacta tttttaaatt
     1441 tgtactttaa caagccgtca tgaagcacag tttctacaac aaaagggata ttcattcagc
     1501 tgttctcctt tcttacgaaa attaattagt tagaagctac gatcaaagtt gaatcacaac
     1561 aaaaaaggca atcaactaag tttttcttaa ttgattgcct ggtatcttct taaagacttg
     1621 aaattccctc aaaaacccga tataatgggt ttacagatat ttaagtatct gattaataaa
     1681 gtaattaaat actttaccaa atttcgggtc tcgacttctt taattgattg gtggtaatca
     1741 attaaggctc gcaacttatt ttcttggcgg aaaataagtt ggtcgtggct ctttttttgt
     1801 attctttatt cagttcgttg tttcgttata tctagtatac cgcttttaaa aaaaataagc
     1861 aacaatttcg ttaaatatat taacgacaat cattgcttct cttcttctat ttgcaactct
     1921 acttgttcta attcattagg aattaagtca ataactaagg ttgagtttgt caattctgca
     1981 attcgttcca atgtttttaa tgtcggatct gattttcctg attcaatcaa tgaataattt
     2041 gatctactca tttctaattc ttgggagaat ttttcttttg atttggtacc tctcatcttt
     2101 tttaagcttt ctctaaatgc tagttccaag tttctccctc ctgtttatgt aagtattttg
     2161 cacgttttcc atttaattat atcgttacac cttgtaaatc acaagtgatt aatcacaaat
     2221 cacttgtgat tagtgattgt tattggtgtt gcctagaacg attctaagca catgtttaat
     2281 tttaaagtgg gcaattatct ttaaaatgtc ctacaatgat tcaataaaag catggctgct
     2341 agtgaaactt gcgaccatgc ttttaaaaat acttaatggg gtcccgagcg ctttagctcc
     2401 ttggaagctg tcagtagtat acctaataat ttatctacat tccctttagt aacgtgtaac
     2461 tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata atagataact
     2521 attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta ctttggcgtg
     2581 tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc gattgaccca
     2641 ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg tggtatggcg
     2701 ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt ccgctggcag
     2761 cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg ttcggtgaat
     2821 atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag acgcatggct
     2881 ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatac tcccaacaat
     2941 tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac
     3001 acgaatgttc agttttaatt tttctgacga taagttgaat agatgactgt ctaattcaat
     3061 agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt tacctgttcc
     3121 aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt ggttgagtac
     3181 tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt aatcactcct
     3241 gaagttatta catctgtaaa taaatacaga agttaaacga tttgtttgta attttagtta
     3301 tctgtttaaa aagtcataag attagtcact ggtaggaatt aatctaacgt atttatttat
     3361 ctgcgtaatc actgttttta gtctgtttca aaacagtaga tgttttatct acattacgca
     3421 tttggaatac caacatgacg aatccctcct tcttaattac aaatttttag catctaattt
     3481 aact
//
LOCUS       DACIWG010000113         3479 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.121, whole genome shotgun sequence.
ACCESSION   DACIWG010000113 DACIWG010000000
VERSION     DACIWG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.121"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..203
                     /locus_tag="IYF54_002635"
     CDS             <1..203
                     /locus_tag="IYF54_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9832897.1"
                     /translation="PLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSL
                     NYRTPIEIFLSYVQEAFYSSLI"
     gene            697..984
                     /locus_tag="IYF54_002636"
     CDS             697..984
                     /locus_tag="IYF54_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832898.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            1002..1424
                     /locus_tag="IYF54_002637"
     CDS             1002..1424
                     /locus_tag="IYF54_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832899.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1444..3036
                     /locus_tag="IYF54_002638"
     CDS             1444..3036
                     /locus_tag="IYF54_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832900.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3292..>3479
                     /locus_tag="IYF54_002639"
     CDS             3292..>3479
                     /locus_tag="IYF54_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832901.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1386 a          537 c          664 g          892 t
ORIGIN      
        1 gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg
       61 attttagaga agtgaatcag acatttattt ccagtgtcag caatcaacgt aatcatattc
      121 caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag
      181 cattttattc tagcttaatt tgacaaatca tatgaaaaaa gtatataaaa aatgtttatg
      241 gatcagctat atcaccctag gtatagctgt taaagagatg ggagaaaaca ttaaccacga
      301 ttctaacaca aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt
      361 tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt
      421 tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa
      481 tacacaaata accttaattt ttaaagagca taaggaaacg tgggaacact ttttaaaaaa
      541 agtgttccaa taggctgtgc ctattacgtt tctctaaaac aaaagtgggt acaaaatatt
      601 cgatttatgg atagtgtatg gatagtaaaa cagctataca tggatagcat atggatagac
      661 tccatagact atccatactt ttttcggagg gtgaaaatgg aaattaaaga gattaaagta
      721 agaggtatta ataaaaaata tgttcaagaa attgatcata gatgcgtaga attaacagaa
      781 caaactggtc aaaaatggag tcgaaatgat tatctaaaac tattgattga aaacgacttt
      841 gaacgtcctt taatggacta caagaaagat caatttgatc gtttattgga aaaatttaca
      901 gatgttcagc tgcataatac aaaagttttg gaagcataca cgaacgaggt caagaactta
      961 attgaaatat taatcgcaca ttaggaagga gataatgttc tatggttaaa gcgatagaaa
     1021 agcatattac attttatgaa gcgcacgatg aagttttaaa acatattatg gaaactcagc
     1081 ctacggtaaa aaactattct gaagccattc gatttttatg tatgaattac gatccagaaa
     1141 ttgaaaaaaa taataatatg acgaacgcaa agttgaacgc aatgggaaag gaaatttcta
     1201 ttttaataga aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatattaa
     1261 ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg tgtggaaggg aaaataaaag
     1321 cgaatcaaac gaagaagtat tctcctaaaa tcaagaaatc aataaatgaa tctgttcatg
     1381 ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag ataaaaagta gaaggggaat
     1441 atgatgaata aatcgcctgc agtcattctc acttgtcaat ttaccttgcc caataaaaaa
     1501 tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga aatggaacga
     1561 cgtacgcctg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
     1621 attcctgaag gagaaacttt ttcggagagt aagacggata aagaattcag cgaaacgtcg
     1681 aatgaagctc aatctttgat gggaaatgga atcaactttg acaagttaga ggaacaagat
     1741 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
     1801 acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag caataaaaaa atatcaaaca
     1861 gagccacaca aaaaagcttc aggaaacctt cctggagttt tttctatggc ttcagacgaa
     1921 gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc aaagaggaca acaaaaaggc
     1981 tcaatcattt atcaagatgt tgtctctcat gataattcgt atcttgaaaa aataggattg
     2041 tacgatccca aaacagatac tttagatgaa gaaggattaa aagcagctgg aagaaaaatg
     2101 atggagacat tattcgagaa agagcaactg aacgaaacag gctactggat ggcaacgatt
     2161 caccgtaata ccaaacatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
     2221 aaaccttaca cggaggttga aaatggtgtg agttatatcg taccgaaagg taagagaaga
     2281 caagcaacat tagacgcaat gaagaccaat tatgtacatg agttagaacg tttttcttat
     2341 gaaaaggatc gaatgattgg tatggagaaa cgaaatttat tgacgcggaa aagtgatttg
     2401 cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt acgatcgaca cgcaatgaac
     2461 ttattaaatg aagtatataa aaccctacct gacaaacgtt cggaatggca ttatggggat
     2521 gaaaaacgaa ccaaactttc accatacaca agagagaaac tcgatgagtt aaccaaatat
     2581 gttttaacaa aagaacctga atatcaagaa tatatacaga tttcaaaagc attgaaagaa
     2641 gaagcaaaaa gtctttatgg ggaaagcaaa agagaatcaa aggatgcaga aaaaaatgct
     2701 ctctttgatt tgaaaaagcg cacaggaaat gccattctaa cagaactgaa gaaacagaat
     2761 agtcaaataa aacctttaat agaaagtatg gatcagatgc aatctattta caatgaacga
     2821 tcctttcgaa cgcttaaaca gttaccaacg aatgaataca aatatagtta cgaagaattc
     2881 atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt gcgtcaatta
     2941 aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagccta cgaagagcaa
     3001 caacgaagaa tccaaataga acaagaaaga cagtaaagac agtaaagtta gcgccttatt
     3061 atactacttg ggaaatgaat gaagagggga tcaataactt cccgtgtaaa agcattgaag
     3121 agtctatttc actcacagaa tgttagttgt taagtgaata aaagaagtaa aagagtcctg
     3181 aacgtaattg tccaggactc tttttttcaa tgaattgttt tccgccaatt caatgagtgg
     3241 tgcgatttgc gactacatta tatcaaaaga aagagaaggg aggaagaaga aatggcttat
     3301 tcaacgattg gagttgttgt tgaaaaaagc agagacaatc tcgtttttgt gacagaaatt
     3361 caaacaggca gagcttttgt cgtcacagac aaagcggcaa aagcgtatca aaatggcgat
     3421 attttaacgt taaatctgac tacgaagacg ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DACIWG010000114         3220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.122, whole genome shotgun sequence.
ACCESSION   DACIWG010000114 DACIWG010000000
VERSION     DACIWG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.122"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            50..1438
                     /locus_tag="IYF54_002640"
     CDS             50..1438
                     /locus_tag="IYF54_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ9832902.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="IYF54_002641"
     CDS             1776..>3220
                     /locus_tag="IYF54_002641"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ9832903.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DACIWG010000115         2999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.123, whole genome shotgun sequence.
ACCESSION   DACIWG010000115 DACIWG010000000
VERSION     DACIWG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.123"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..586)
                     /locus_tag="IYF54_002642"
     CDS             complement(<1..586)
                     /locus_tag="IYF54_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832904.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="IYF54_002643"
     CDS             complement(1224..1481)
                     /locus_tag="IYF54_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ9832905.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="IYF54_002644"
     CDS             complement(1474..1743)
                     /locus_tag="IYF54_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ9832906.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1874..2053)
                     /locus_tag="IYF54_002645"
     CDS             complement(1874..2053)
                     /locus_tag="IYF54_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832907.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2434..>2999)
                     /locus_tag="IYF54_002646"
     CDS             complement(2434..>2999)
                     /locus_tag="IYF54_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832908.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          914 a          562 c          396 g         1127 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
     1921 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
     1981 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
     2041 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
     2101 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
     2161 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
     2221 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
     2281 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
     2341 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
     2401 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
     2461 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
     2521 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
     2581 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
     2641 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
     2701 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
     2761 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
     2821 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
     2881 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
     2941 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttctt
//
LOCUS       DACIWG010000116         2995 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.124, whole genome shotgun sequence.
ACCESSION   DACIWG010000116 DACIWG010000000
VERSION     DACIWG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.124"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..178
                     /locus_tag="IYF54_002647"
     CDS             <1..178
                     /locus_tag="IYF54_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ9832909.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
     gene            complement(369..1901)
                     /locus_tag="IYF54_002648"
     CDS             complement(369..1901)
                     /locus_tag="IYF54_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9832910.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            2135..2836
                     /gene="nagB"
                     /locus_tag="IYF54_002649"
     CDS             2135..2836
                     /gene="nagB"
                     /locus_tag="IYF54_002649"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ9832911.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
BASE COUNT          909 a          521 c          608 g          957 t
ORIGIN      
        1 tgtatttatg tttatgtact ttattggagg tgcggctggt tcattgctag ggacgacaat
       61 gtggcagcag tatggctggt taggtgtaac ggttagcggg cttgtttttc aaggatgtgc
      121 gttcttcttt caaacagttg tttttaaagg aaagagaaat ctcgaaaata aaaaatagac
      181 gaaaagaggt tgtgaaaagc tgttctgcat caagtaataa gaatagccaa ataaaaatag
      241 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctaattg gccaccattt
      301 attcagaaga aggagttgtg acaaaacttt tgtcacagct cctttttttc aactattcaa
      361 tcaaacgttt attttggttc aatctcaatg actcgttgca caagatcttg ataaaattct
      421 tgttcatgag agacgatgat tactgttcct ggaaatttct gtatcccgtc ttgtaagctt
      481 tctttcgttt cttgatcgat atggttcgtc ggttcatcga gaatcagtaa attaccttgg
      541 ttcattgtca actgtgcaag tttcacctta gtttgttccc caccacttaa tgtagtgaga
      601 ggttcacttg ctaattgatt aactaaccct gctttagcta gttggcgacg taattctttg
      661 atagtcgctt ttggaaattg actgcttaaa tattgtagtg gtgtttctaa tggattttcc
      721 cagtacagat cttgtgaaaa gtaattgatt tttgtgttat ctgggaagtg gaaatcgcca
      781 cttatttttt caatctcacc gatcagtgtt tttaataaag tagattttcc aatcccgttg
      841 aagcctcgaa tggcaatatt ttcaccaaag cggatggtca aattgatggg tgcaagtagc
      901 ggtcgatcat atccgatgac taaatcagtt gtttccactg ctaatgtcgt gacaataggc
      961 gagaatggaa aatcaaagac agctttactt gctgaagctg gaggcgtcag ccgttcgatt
     1021 ttatctaatt gtttttgtcg gctttttgcc atcgttgatc gggaaccggc tttgtatttt
     1081 cgaatataag cttcggtctt ttcgatttgt ttttgttgag catgatattg gcgcaaatag
     1141 ctttcgcgat tttgttcctt ttgtttcaat gcttgtttta gatggccagt atacttagta
     1201 atctttccaa actcgatgtc tgcgatatgg gtggtgatct gatcaagaaa ttcttggtca
     1261 tgggaaatca cgatatacgc accgtcaaaa tcctgaagaa actgcgtcag ccattggata
     1321 tgctgatcat ctaagtgatt ggttggttca tctaagatca gaacatctgg gttttctaaa
     1381 agtagttttg ctaaaatcac ctttgaacgt tgtccaccac ttagattttt caacagtgaa
     1441 tctaaaccta agacatcgat ccccaacccg ctgctcatct cagcaactaa ggtgtcgatt
     1501 tgataaaaca ctccttggtc taattccgtt tggagtttcc cagctttttc cagcaaggaa
     1561 tcatcgaagg tctcgctata tgtttggtaa agaaaagcaa ttttttcttc cttttcgtat
     1621 tcttttgcgt acgcagtttt tagaaattct tcgattgtta atgtttcatc gacttccaca
     1681 tattgatcga ggtaaccttt gtgaatattt ttttgccaat ggatctgtcc gtcatctggt
     1741 aagatctcac cagtgatgat tttaatcaat gtacttttac cggctccgtt ttgtccaatc
     1801 agcccaagat gttcgccagc atttaattgc agatcgacac cttcatagag gattttgtcg
     1861 ccaaattgct gggtaatgtt gtttaatgtc agtatactca taataactcc tttttcataa
     1921 aaaaactgca gacataaagc gtccacagtg aatcacttgc ttggttttca tgcaacatgg
     1981 attgtttgat tgaaccatct tatgaaacct aacacggatt cctatttatt gtcaagaaaa
     2041 tgaaaaaaat gcaatttggt ctatactaat tattgtaaaa agaaaaaaag aatgctataa
     2101 ttgggctata ccaaaatata aaggagcaat cactatggaa atcattcgtg taaaaaacgc
     2161 tgaagaaggc ggaaaaaaag catttgaaat catcaaagaa ggtatgaaca aaggtgcaaa
     2221 agtattagga ttggcaacag gaagcacacc agttacttta tataaagaaa tgacttcaag
     2281 cgatttggat ttcagtgaaa tgacttctgt aaacttagat gagtacgttg gactaggcgg
     2341 ttcagatgaa caaagctacc gtcatttcat ggatgtccaa ttatttgata aaaaaccatt
     2401 caaagaaacg tttgtaccag atggaaaagc agaagatctg gaagcagcat gcaaaaagta
     2461 tgatcaaatc atcgatgcac atccaatcga tatccaaatc ttaggaatcg ggcaaaatgg
     2521 acatatcggt ttcaatgaac ctggtgcttc atttgacgga acgacttcag tagttgactt
     2581 aacagaatca acaatcaatg caaacaaacg attcttcgat aaagcagaag atgtaccaac
     2641 aaaagctgtt tcaatgggaa tcggttcaat tatgaaaagt aaagaaatcg tcttgatggc
     2701 atttggcgaa agcaaagctg atgcaatcaa agggatgatt gaaggacctg taacgaatca
     2761 tttgccagca agtgtattgc aaaatcacga caatgttgtt gtgatcatcg atgaagaagc
     2821 agcaagtaag ttgtaatttt caatagtagg ctaaagaggt tgtgaaatca gcgttttgac
     2881 ctgagtatta gagaagacaa aggaaaaata gctccccata ttttgccgtt tgttgagtaa
     2941 taccgagggt cagctgattg aacaccgttt attcataaaa aggactatga caaaa
//
LOCUS       DACIWG010000117         2559 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.125, whole genome shotgun sequence.
ACCESSION   DACIWG010000117 DACIWG010000000
VERSION     DACIWG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.125"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..902)
                     /locus_tag="IYF54_002650"
     CDS             complement(<1..902)
                     /locus_tag="IYF54_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ9832912.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IYF54_002651"
     CDS             1185..2153
                     /locus_tag="IYF54_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832913.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IYF54_002652"
     CDS             2266..2544
                     /locus_tag="IYF54_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ9832914.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT          837 a          466 c          524 g          732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaa
//
LOCUS       DACIWG010000118         2483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.127, whole genome shotgun sequence.
ACCESSION   DACIWG010000118 DACIWG010000000
VERSION     DACIWG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.127"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(1014..1304)
                     /locus_tag="IYF54_002653"
     CDS             complement(1014..1304)
                     /locus_tag="IYF54_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832915.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IYF54_002654"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IYF54_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACIWG010000119         2469 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.128, whole genome shotgun sequence.
ACCESSION   DACIWG010000119 DACIWG010000000
VERSION     DACIWG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.128"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..418
                     /locus_tag="IYF54_002655"
     CDS             <1..418
                     /locus_tag="IYF54_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832916.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IYF54_002656"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IYF54_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ9832917.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IYF54_002657"
     CDS             1305..2459
                     /locus_tag="IYF54_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ9832918.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          852 a          443 c          521 g          653 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtata
//
LOCUS       DACIWG010000120         2422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.129, whole genome shotgun sequence.
ACCESSION   DACIWG010000120 DACIWG010000000
VERSION     DACIWG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.129"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..290
                     /locus_tag="IYF54_002658"
     CDS             <1..290
                     /locus_tag="IYF54_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832919.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="IYF54_002659"
     tRNA            complement(803..876)
                     /locus_tag="IYF54_002659"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="IYF54_002660"
     CDS             complement(1065..2084)
                     /locus_tag="IYF54_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ9832920.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="IYF54_002661"
     CDS             complement(2095..2301)
                     /locus_tag="IYF54_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ9832921.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          811 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tc
//
LOCUS       DACIWG010000121         2399 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.130, whole genome shotgun sequence.
ACCESSION   DACIWG010000121 DACIWG010000000
VERSION     DACIWG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.130"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            207..2069
                     /locus_tag="IYF54_002662"
     CDS             207..2069
                     /locus_tag="IYF54_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9832922.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="IYF54_002663"
     CDS             2069..>2399
                     /locus_tag="IYF54_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9832923.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DACIWG010000122         2388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.131, whole genome shotgun sequence.
ACCESSION   DACIWG010000122 DACIWG010000000
VERSION     DACIWG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.131"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(493..1785)
                     /locus_tag="IYF54_002664"
     CDS             complement(493..1785)
                     /locus_tag="IYF54_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832924.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IYF54_002665"
     CDS             complement(2059..2319)
                     /locus_tag="IYF54_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832925.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          788 a          448 c          360 g          792 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaa
//
LOCUS       DACIWG010000123         2365 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.132, whole genome shotgun sequence.
ACCESSION   DACIWG010000123 DACIWG010000000
VERSION     DACIWG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.132"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            150..1451
                     /locus_tag="IYF54_002666"
     CDS             150..1451
                     /locus_tag="IYF54_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9832926.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="IYF54_002667"
     CDS             complement(1555..1755)
                     /locus_tag="IYF54_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ9832927.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          768 a          454 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DACIWG010000124         2351 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.133, whole genome shotgun sequence.
ACCESSION   DACIWG010000124 DACIWG010000000
VERSION     DACIWG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.133"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..544
                     /locus_tag="IYF54_002668"
     CDS             <1..544
                     /locus_tag="IYF54_002668"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9832928.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IYF54_002669"
     CDS             complement(1017..1589)
                     /locus_tag="IYF54_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832929.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IYF54_002670"
     CDS             complement(1605..2210)
                     /locus_tag="IYF54_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ9832930.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACIWG010000125         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.134, whole genome shotgun sequence.
ACCESSION   DACIWG010000125 DACIWG010000000
VERSION     DACIWG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.134"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(51..1598)
                     /locus_tag="IYF54_002671"
     CDS             complement(51..1598)
                     /locus_tag="IYF54_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ9832931.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYF54_002672"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYF54_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ9832932.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IYF54_002673"
     CDS             complement(2043..2237)
                     /locus_tag="IYF54_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832933.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACIWG010000126         2005 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.136, whole genome shotgun sequence.
ACCESSION   DACIWG010000126 DACIWG010000000
VERSION     DACIWG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.136"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            306..848
                     /locus_tag="IYF54_002674"
     CDS             306..848
                     /locus_tag="IYF54_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832934.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1004..1453
                     /locus_tag="IYF54_002675"
     CDS             1004..1453
                     /locus_tag="IYF54_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ9832935.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1483..>2005)
                     /locus_tag="IYF54_002676"
     CDS             complement(1483..>2005)
                     /locus_tag="IYF54_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ9832936.1"
                     /translation="EAIVTDLTYVRVRKKWHYICLILDLFNREIIGYSCGEKKDASLV
                     KEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVEST
                     YKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRIL
                     DNERGSDTSGEAA"
BASE COUNT          713 a          312 c          378 g          602 t
ORIGIN      
        1 aggcttacca gcgagataga gatcgacgat ttgttgtttg aattcttttg aataggttct
       61 tcgttgacgt cttggcatga aaaatcctcc agtgtgtttt ttattattct acacacctta
      121 ttttttctgt ctagtctagt gtagccgatt caagttcttc gtgttctctt agacataaaa
      181 attcctcctg gtatgttttc ttctagtcta cacaccttaa tttttctgtc tagttaattg
      241 tagcctatcc agtttggaaa aataaaaaat tatatttcat caacgtttca taaaaggtgt
      301 gtagaatgtt attgaaagta gctataaaat gctattgtgt aaaaaataaa ggaggaataa
      361 tttatgaaaa aatagtacca agaggagcat gcctattact tgcattatgt ttgagcttaa
      421 gcgcagttgg gtgtcaaaaa agtaatgaaa acaataaaat aaaagaagga caatccataa
      481 gttctgaaga ggaaggcatg tcaattaatg caaatggcaa aaatgaaact tttaagccat
      541 cagattatac attggaggca aagaaagaat atgtttatga atatttaggg cttaaattta
      601 aactctcaga caaatttaga aattatatag ctgataaaaa gatagcgatg ttagatgatc
      661 aaagtcctat agataaagaa ctaaagtatg ctattttgac atttgagaaa atgacagaag
      721 aacaaaagaa tgcagttata gaaaagatgg gcgatggata taaaaattgg caaaatgagc
      781 ttgaaagaat tgggactatt ggtatatttg aaaaaaatac atcagaagaa aaaaatctaa
      841 aattataaaa tgtgatactc atactaaaat aggagtttca agtgatggaa aatacgattg
      901 ctatttaagc acaaatagcg gagctgaaag taatcttctt gatgaattaa aaagaactga
      961 gattcaaatt atagataaaa aagaacgtcc taaaaatggt tttgtgctat cagaaaaaac
     1021 tgatttagaa aatacagaag catttaacaa agaatcggtt aaagatttac gtaaactacc
     1081 aacaaaggac ataaatggca aggattttac aagtaaagat tttgagaagt atgatcttac
     1141 tatggtaaat gtatttgcaa cttggtgtac agcttgtgta aaggaaattc ctgatttggt
     1201 tgaggttcag aatgaaatga aaagcaaggg tgtaaatatc gttggagttg ttacagatcc
     1261 tgttgatgat aacggtgaaa acaaggaagc aattgaaaag tcaaagttaa tacatgaaaa
     1321 gacaaaggct tcatatcctt tcttaatgcc agatggaatg gcaacacttt ttttgacaaa
     1381 aattataagg tgttgtgaat gctgcggtgt tatggctccg attgcccttg acaatgagca
     1441 tcctcggatc tgattttctc cggcagaagc acaatcattc aattacgctg cctctccaga
     1501 ggtatcagat ccgcgctcat tatcaaggat tcgcttcgcc aatctctgtt gtcgaatttg
     1561 gatcggtgtt tcatacgcca acgtgccatg taagcgtaga tagttccacc aatgcacata
     1621 atcaaacaat tctagacgta gctgtgccag tgtctcaaat tggtattgat gcacgaattc
     1681 tactttgaca gatttatagg ttgattccac aacggcatta tcatacggac aacccttcct
     1741 actcaatgaa cgagtaattc caaaaccatt cagaatctca tgaatggttt ggttatcaaa
     1801 ttcctttcca cggtctgtat gaaaaagctt gacgtctgtt aaagaatacg gtatccgtcc
     1861 aaaggcttct tttaccaatg aggcatcttt cttctcacca caggaataac caataatttc
     1921 tcgattaaac aaatcaagta ttaagcagat ataatgccac tttttcctca cgcgaacata
     1981 agtaagatcc gtaacgatgg cttcc
//
LOCUS       DACIWG010000127         1916 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.138, whole genome shotgun sequence.
ACCESSION   DACIWG010000127 DACIWG010000000
VERSION     DACIWG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.138"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..398
                     /locus_tag="IYF54_002677"
     CDS             6..398
                     /locus_tag="IYF54_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9832937.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="IYF54_002678"
     CDS             399..1838
                     /locus_tag="IYF54_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ9832938.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          807 a          138 c          325 g          646 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat gatggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagt
//
LOCUS       DACIWG010000128         1758 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.139, whole genome shotgun sequence.
ACCESSION   DACIWG010000128 DACIWG010000000
VERSION     DACIWG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.139"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            514..1644
                     /locus_tag="IYF54_002679"
     CDS             514..1644
                     /locus_tag="IYF54_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ9832939.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          520 a          284 c          360 g          594 t
ORIGIN      
        1 tataaaaaaa cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt
       61 tgcaacggga ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta
      121 aatttcacta tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac
      181 tcgtaaatag aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt
      241 tgcccagaaa cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt
      301 tccaatagct aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg
      361 atgctaggtc agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac
      421 tgacttattt gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag
      481 gagatgcggt gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg
      541 atcggtcttt tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat
      601 acaaaaagtc tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc
      661 ttagctcgtt cagtcaaact acactattta cttcacccta aaatagcagg atacggttta
      721 gccttatcga ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac
      781 ggcgcacaac gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat
      841 ctttttttga ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat
      901 ctaaaaaaac cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag
      961 attgctggag cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt
     1021 ccatttaaaa aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca
     1081 ggcggggtat tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat
     1141 gaaaggatcg cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg
     1201 acccattcgt tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt
     1261 atcacaaaaa aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca
     1321 gaagagcttg ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg
     1381 agaatctttt gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga
     1441 ttcggaactt tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg
     1501 ccaatgactg gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt
     1561 tctttaggaa tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg
     1621 ctctatcgac ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata
     1681 aagtacaatc ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa
     1741 agtatctcaa aaaatcat
//
LOCUS       DACIWG010000129         1722 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.140, whole genome shotgun sequence.
ACCESSION   DACIWG010000129 DACIWG010000000
VERSION     DACIWG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.140"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(32..580)
                     /locus_tag="IYF54_002680"
     CDS             complement(32..580)
                     /locus_tag="IYF54_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832940.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(581..1438)
                     /locus_tag="IYF54_002681"
     CDS             complement(581..1438)
                     /locus_tag="IYF54_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ9832941.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          484 a          347 c          247 g          644 t
ORIGIN      
        1 taaaggtagt tacttagttc ttctttgatt attatctaac tttattattc attccccacc
       61 ctttgcgcag tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc
      121 cacgatcatc taattctgga acaattggat atttttctct gaacaagtct agttctttca
      181 ccacgtcatt aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat
      241 ctaaagcttt tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa
      301 ctctggaagt cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct
      361 tatcaaactt tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa
      421 cattggagga agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg
      481 gtgtaattgc ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt
      541 tattgttccc aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc
      601 aattctcgtg ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat
      661 cccattgttt taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc
      721 gttttaagcg cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt
      781 gggcttgttt atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa
      841 caccagcaac atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat
      901 attgcatcgc atttctcatc gattttactt gggtctgtaa gacgacgatt gcccagtccg
      961 caatgtacat cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga
     1021 cgtcaaactc atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg
     1081 aatcattata ttggtctttg atccaatcat catacatttc aaaggacatg tctgctggaa
     1141 gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca
     1201 acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt
     1261 tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt
     1321 caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc
     1381 acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc gctaccacgt
     1441 tcctacctct tttcttttag caatattcat ttgatactat tataacatat ttatttttag
     1501 ttactaccaa aaaaaagata tatatgtact accttataat taataaggaa aaggtagtac
     1561 ataaataagg aaaagctgaa atatattgat ttatagcttt cctcaaagta atgacgttat
     1621 ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt
     1681 cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tc
//
LOCUS       DACIWG010000130         1720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.141, whole genome shotgun sequence.
ACCESSION   DACIWG010000130 DACIWG010000000
VERSION     DACIWG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.141"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            386..574
                     /locus_tag="IYF54_002682"
     CDS             386..574
                     /locus_tag="IYF54_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ9832942.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="IYF54_002683"
     CDS             652..1665
                     /locus_tag="IYF54_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ9832943.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
BASE COUNT          577 a          278 c          361 g          504 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttca
//
LOCUS       DACIWG010000131         1703 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.142, whole genome shotgun sequence.
ACCESSION   DACIWG010000131 DACIWG010000000
VERSION     DACIWG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.142"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..588
                     /locus_tag="IYF54_002684"
     CDS             <1..588
                     /locus_tag="IYF54_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832944.1"
                     /translation="EVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDI
                     PRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLW
                     KELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQ
                     EKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(585..1181)
                     /locus_tag="IYF54_002685"
     CDS             complement(585..1181)
                     /locus_tag="IYF54_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9832945.1"
                     /translation="MQQNPSIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQE
                     VLDFVRDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLD
                     KFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLV
                     YRSIVQDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
     gene            1242..>1703
                     /locus_tag="IYF54_002686"
     CDS             1242..>1703
                     /locus_tag="IYF54_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAQ9832946.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKT"
BASE COUNT          563 a          279 c          328 g          533 t
ORIGIN      
        1 gaagtacttc ctacttttct tgctggaaag cttggaattg atttatcaga tagccagcat
       61 tatgatgaaa acttagatga tttaattaca actatatttg gtgttaagag aaaaccagca
      121 ttaggtgata tacctaggca tattaaaggc agtcctttac tacctataga acataatgaa
      181 aataaagata ttgaaatctt aggaattatc actgataaag taactgtacc taaaatggat
      241 ggctcaaggg gttctgcatt atataaaatt ccatttagat taaatagagt accaagtcag
      301 ctttggaaag agttgtttgt tagaaattgg aattcaccac cacgatatac aacaatgcat
      361 agaccaggaa ttgcttctgt tgtagacgat gaaattttac ttaatggaac aactattgag
      421 gaagtccggg aatatcatcg ggaaacttta aaactttgca ttgaaaaaag taatactcaa
      481 gaaaaaaaaa tattgctaga ggaggaacaa aaaaaagaga gagagaatga gcgagtcaga
      541 tcacataaag aaagtgttca gaaattagca gatgaattag agttctagtt gatagtcagt
      601 tctttcttaa ttcgatacac agtttgtcta gttacattat agtctgttgc tatttttgaa
      661 atagcaacac cattctcctg gtcctgaacg atgcttctat aaacaaggcg acgttgagga
      721 tcttttgtgt tcgcactgta taacttagga cgtcctttat agacgccgtt tgctttagca
      781 atctttatcc cttgtgcctg gcgtcgtttt gattcagtac gctcttgttc agcaatcatg
      841 gccaagattt gaatgattag gtctttgata aatttatcca ggagaggatt tccgattgct
      901 tcagccatga tcggtaaatt agtgataata agtcgcacat ttttcttctt tagataattt
      961 actgaatcga taatctcgtc ataattacgt ccaagtcgat cgatagcctc tacaataaaa
     1021 atatcttggt ctctaacaaa atctagaact tcctgaaaaa tcggtcgatg agtaatagtc
     1081 gctccagatt gtttctcgat gaaaattttt tcagcaccaa attttctcat ttcctcgatt
     1141 tgacgttgtt cattttgatc gatcgatggg ttctgttgca aagttttcca aaaaatctat
     1201 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     1261 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     1321 gctatcgtga agttcaggaa ttgttatatg atcgtggaat aaatgtttgt catactacga
     1381 tttatcgttg ggtacaagag tacagcaaag tcctctatta tctttggaag aagaaaaata
     1441 gacaatcctt ctattcatgg aaaatggacg aaacctatat caaaattaag ggacgttggc
     1501 attatcttta tcgtgcaatt gatgcggacg gcttaacctt agatatctgg ttacgaaaga
     1561 aacgggatac gcaagcagcc tatgctttct taaaacgact ccataaacag tttggtgagc
     1621 cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc cgcctttaga aagttacaga
     1681 gtgtgggttt atatactaag aca
//
LOCUS       DACIWG010000132         1655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.143, whole genome shotgun sequence.
ACCESSION   DACIWG010000132 DACIWG010000000
VERSION     DACIWG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.143"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            48..1210
                     /locus_tag="IYF54_002687"
     CDS             join(48..324,324..1210)
                     /locus_tag="IYF54_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9832947.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(1575..1648)
                     /locus_tag="IYF54_002688"
     tRNA            complement(1575..1648)
                     /locus_tag="IYF54_002688"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1612..1614),aa:Asn,seq:gtt)
BASE COUNT          589 a          296 c          326 g          444 t
ORIGIN      
        1 aaattaaggt gtgtagacta gaagaaaaca taccaggagg aatttttatg tctaagagaa
       61 cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac ttagctggaa
      121 agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt gacaaatggg
      181 taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg cctgaacaaa
      241 aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat gatattttaa
      301 agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata agcatcttta
      361 ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt attatcaatc
      421 aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat ttatccgcag
      481 ccgaaaggcc tacggctcaa gaaaaataaa aaaagcctta tcaaaacgag gcattcagat
      541 cagccgacga aaaattagta gaatcatgaa aaatagagga ttaaaatcga gctatactgt
      601 tgcttatttt aaagtacatc attctacttg caatgaagcc aaaacgacaa acgtattgaa
      661 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
      721 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      781 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      841 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      901 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      961 ctgtcctttt gataatgccg tagctgaatc aacttataag tcgttgaaag tagaatttgt
     1021 ctatcaatac acatttgaaa ccttacaaca attggatttg gagttatttg actatgtcaa
     1081 ttggtggaac caccttcggt tgcacggtac acttggctac gagacaccgg ttggttaccg
     1141 taaccagaga ttggcgcagc gaatccttga taatgagctc ggatgtgcta acgctagcga
     1201 ggcagtctaa ctttaactgt tagctcctgc cgaagatcgt cacatccgag gaggctcatt
     1261 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
     1321 aaaactgttg ccattccaat gagaaaaagc atggaatgcg ctgcatcagg tatcgaggct
     1381 tagaaaaaaa tcaggtgcat acgatgcttg tttttgccga aatgagtctg aaaaaattgg
     1441 tcacttagca gtgggagcat atgtcttcat attttttatt atacaaaaat agacaaaagt
     1501 atcagtctac actcttagta aagaggaaac tgatactttt gtctacagta tgatatttct
     1561 tacatctact tttactccgg cagtaggact cgaacctacg acatcatgat taacagtcat
     1621 gcgctactac caactgagct atgccggaat aacat
//
LOCUS       DACIWG010000133         1642 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.144, whole genome shotgun sequence.
ACCESSION   DACIWG010000133 DACIWG010000000
VERSION     DACIWG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.144"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..>1642)
                     /locus_tag="IYF54_002689"
     rRNA            complement(<1..>1642)
                     /locus_tag="IYF54_002689"
                     /product="23S ribosomal RNA"
BASE COUNT          348 a          507 c          350 g          437 t
ORIGIN      
        1 taagtcctcg atcgattagt atcagtccgc tccatacatc actgtacttc cactcctgac
       61 ctatctacct gatcatctct cagggatctt actttcttaa agaaatggga aatctcatct
      121 tgaggtgggc ttcacactta gatgctttca gcgtttatcc cttccctaca tagctaccca
      181 gcaatgccct tggcagaaca actggtacac cagcggtaag tccatcccgg tcctctcgta
      241 ctaaggacag ctcctctcaa atttccaacg cccgcgacgg atagggaccg aactgtctca
      301 cgacgttctg aacccagctc gcgtgccgct ttaatgggcg aacagcccaa cccttgggac
      361 cgactacagc cccaggatgc gacgagccga catcgaggtg ccaaacctcc ccgtcgatgt
      421 ggactcttgg gggagataag cctgttatcc ccagggtagc ttttatccgt tgagcgatgg
      481 cccttccatg cggaaccacc ggatcactaa gcccgacttt cgtccctgct cgacttgttg
      541 gtctcgcagt caagctccct tctgccttta cactcttcga atgatttcca accattctga
      601 gggaaccttt gggcgcctcc gttacctttt aggaggcgac cgccccagtc aaactgccca
      661 tctgacactg tctcccacca cgattagtgg tgcgggttag agtggccata acgcaggggt
      721 agtatcccac cagcgcctcc atcgaaacta gcgttccgat ttctacggct cctacctatc
      781 ctgtacatgc ggtacagaca ctcaatatca aactacagta aagctccatg gggtctttcc
      841 gtcctgtcgc gggtaacctg catcttcaca ggtactaaaa tttcaccgag tctctcgttg
      901 agacagtgcc caaatcgtta cgcctttcgt gcgggtcgga acttacccga caaggaattt
      961 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcaa ttcgtacctt
     1021 cgcttgcgct aagcactcct cttaaccttc cagcaccggg caggcgtcag cccctatact
     1081 tcatcttacg attttgcaga gacctgtgtt tttgataaac agtcgcttgg gcctattcac
     1141 tgcggctgat cgtatgatca gcaccccttc tcccgaagtt acggggtcat tttgccgagt
     1201 tccttaacga gagttctctc gctcacctta ggattctctc ctcgactacc tgtgtcggtt
     1261 tgcggtacgg gccgctgttt tctcactaga agcttttctc ggcagtgtga catcaggaac
     1321 ttcggtacta ttatttccct ccccatcaca gcttgtcctt aagaaaagaa gcatttgact
     1381 cctcttcaga cttactgctt ggacagacat ttccgatcgt ctgcattcct tagcctcctg
     1441 cgtccctcca ttgctcaaac aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg
     1501 cctacgcctg tcggcctcgg cttaggtccc gactaaccct gggcggacga gccttcccca
     1561 ggaaacctta gtcattcggt ggacaggatt ctcacctgtc tttcgctact cataccggca
     1621 ttctcacttc taagcgctcc ag
//
LOCUS       DACIWG010000134         1636 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.145, whole genome shotgun sequence.
ACCESSION   DACIWG010000134 DACIWG010000000
VERSION     DACIWG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.145"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(183..1502)
                     /locus_tag="IYF54_002690"
     CDS             complement(183..1502)
                     /locus_tag="IYF54_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ9832948.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DACIWG010000135         1624 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.146, whole genome shotgun sequence.
ACCESSION   DACIWG010000135 DACIWG010000000
VERSION     DACIWG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.146"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..485
                     /locus_tag="IYF54_002691"
     CDS             <1..485
                     /locus_tag="IYF54_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ9832949.1"
                     /translation="KKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTD
                     VKLFHTDWGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVAESTYKSVKVEFVHQYQ
                     FETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA
                     "
     gene            592..807
                     /locus_tag="IYF54_002692"
                     /pseudo
     CDS             592..807
                     /locus_tag="IYF54_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1098..1622)
                     /locus_tag="IYF54_002693"
     CDS             complement(1098..1622)
                     /locus_tag="IYF54_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832950.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          542 a          257 c          261 g          564 t
ORIGIN      
        1 ggaaaaagtg gcattatatc tgcttaatac ttgatttgtt taatcgagaa attattggtt
       61 attcctgtgg tgagaagaaa gatgcctcat tggtaaaaga agcctttgga cggataccgt
      121 attctttaac agacgtcaag ctttttcata cagactgggg aaaggaattt gataaccaaa
      181 ccattcatga gattctgaat ggttttggaa ttactcgttc attgagtagg aagggttgtc
      241 cgtatgataa tgccgttgcg gaatcaacct ataaatctgt caaagtagaa ttcgtgcatc
      301 aataccaatt tgagacactg gcacagctac gtctagaatt gtttgattat gtgcattggt
      361 ggaactatct acgcttacat ggcacgttgg cgtatgaaac accgatccaa attcgacaac
      421 agagattggc gaagcgaatc cttgataatg agcgcggatc tgatacctct ggagaggcag
      481 cgtaattgga atgattgtgc ttctgccgga gaaaatcaga tccgaggatg ctcattgtca
      541 agggcaatcg gagccataac accgcagcat tcacaacacc ttataatttt tgtcaaaaaa
      601 agtgttgcca ttccaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      661 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      721 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      781 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca
      841 caaaattatt gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt
      901 tttctattat tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt
      961 aaatattaag atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac
     1021 aaaatatgtg atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt
     1081 atctagtaat ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag
     1141 gcttttttta tatcttctaa attagaagat atttgtatat caatatggaa tatattacct
     1201 tttttgtatt tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt
     1261 atcgaggcag ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac
     1321 ttgtcacatt tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg
     1381 cacgactcac ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca
     1441 acatctaaac tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa
     1501 tttatctttg gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct
     1561 ctatcaaaat agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc
     1621 aaac
//
LOCUS       DACIWG010000136         1519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.147, whole genome shotgun sequence.
ACCESSION   DACIWG010000136 DACIWG010000000
VERSION     DACIWG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.147"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1103)
                     /locus_tag="IYF54_002694"
     rRNA            complement(<1..1103)
                     /locus_tag="IYF54_002694"
                     /product="23S ribosomal RNA"
     gene            complement(1432..>1519)
                     /locus_tag="IYF54_002695"
     rRNA            complement(1432..>1519)
                     /locus_tag="IYF54_002695"
                     /product="16S ribosomal RNA"
BASE COUNT          356 a          417 c          283 g          463 t
ORIGIN      
        1 cctagttgtc tgtgcaaccc cacatccttt tccacttaac atatattttg ggaccttagc
       61 tggtggtctg ggctgtttcc ctttcgacta tggatcttat cactcacagt ctgactcccg
      121 gatatgaatg aatggcattc ggagtttatc tgaattcggt aacccgagat gggcccctag
      181 tccaaacagt gctctacctc catcattctc aattccgagg ctagccctaa agctatttcg
      241 gagagaacca gctatctcca agttcgtttg gaatttctcc gctacccaca cctcatcccc
      301 gcacttttca acgtacgtgg gttcggtcct ccagtgcgtt ttaccgcacc ttcaacctgg
      361 acatgggtag atcacatggt ttcgggtcta cgactacata ctcaaacgcc ctattcagac
      421 tcgctttcgc tgcggctccg tctcttcaac ttaacctcgc atgcaatcgt aactcgccgg
      481 ttcattctac aaaaggcacg ccatcaccca ttaacgggct ttgacttgtt gtaggcacac
      541 ggtttcaggt tctatttcac tccccttccg gggtgctttt cacctttccc tcacggtact
      601 ggttcactat cggtcactag ggagtattta gccttgggag atggtcctcc cggattccga
      661 cggaatttct cgtgttccgc cgtactcagg atcctcctag gtgtcttcca catttcgtct
      721 acggggtttt taccctcttt gactgacttt tccaagtcat tcgactatct gaaagaacta
      781 ccatattgga gtcctacaac cccaacaagc aagcttgctg gtttgggctt ttcccgtttc
      841 gctcgccgct actcagggaa tcgaattttc tttctcttcc tgcaggtact tagatgtttc
      901 agttctctgc gtctacctcg aatgtgctat gtattcacac aatcgtaaca tcctattaaa
      961 gatgttgggt tcccccattc ggaaatctct ggatcatagc ttacgtacag ctccccaaag
     1021 catatcggtg ttagtcccgt ccttcatcgg ctcctagtgc caaggcatcc accgtgcgcc
     1081 cttattcact taaccttatc aaccttacgg ttgggttttg atccttcttc tagcgataga
     1141 agttagatca agaaaataag caattgaact tattaaaaaa ctctatccag ctcacaaatc
     1201 agcctgtacg gcaaaaagat cgatcgtctc ataaaatcaa agattttatg gccgtccgcc
     1261 tattttgctt caggctaaac aatttgttca gctttcaaat tcaacgcggt gttctcggtt
     1321 tgtattactt acatttactt caaatatcca gttttcaatg aacaaaagtg tttgagagta
     1381 aacctctcaa aactgaacaa agtaaaaaca aattgtgtag tctccgtaat attccttaga
     1441 aaggaggtga tccagccgca ccttccgata cggctacctt gttacgactt caccccaatc
     1501 atctatccca ccttaggcg
//
LOCUS       DACIWG010000137         1482 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.148, whole genome shotgun sequence.
ACCESSION   DACIWG010000137 DACIWG010000000
VERSION     DACIWG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.148"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            367..921
                     /locus_tag="IYF54_002696"
     CDS             367..921
                     /locus_tag="IYF54_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ9832951.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1175..>1482
                     /locus_tag="IYF54_002697"
     CDS             1175..>1482
                     /locus_tag="IYF54_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832952.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT          592 a          156 c          256 g          478 t
ORIGIN      
        1 atgaaaagtt tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
       61 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      121 tctaagaact tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca
      181 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      241 tataaaatca taaactaaac cccttgattt tcttcacttt taacagagta tatatataag
      301 ttcatgatta tggtaaaata tatatataat catgaactca gaatattttt aagaagggaa
      361 acaatagtga gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag
      421 ttttggctaa gaagaacaag taatccagaa agagatagtt ttttattttt gtttggaatc
      481 aataatgggt tacgtatgtc agatattata ggattaaaag ttggagacat acgaggtaaa
      541 agtaagccta ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat
      601 ttgagagaag agattttatt atatactgaa ggtaaagaag aaaatgattg gttatttcca
      661 agtcggcaac aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata
      721 gcagaaaaat taggaagaga tgatatagga acacatacat taagaaaaac ttttgggtat
      781 cactactaca agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg
      841 caagcaatta caaaaaggta cattggtata actgaagatg aaataggggc atcgttacgt
      901 ggttttaaat tgggaggcta attgagatat taggatatat tttttgtgta taatgaaaac
      961 ttagaaggtg agattaatga agataactga attttcggaa gtatttaaaa cgtttagtaa
     1021 catttacaat agaataccag agatagaaca aaatgttgta gttgataatg attattatat
     1081 agagaatgat tttaataaac caagaaaata tgacaaccta tacaatttta tacacaatag
     1141 aacaacaata aataacaaca gtaacactcg agttttgatt aattcagcat attttcatat
     1201 taaatattta aagaaatatg agtatgaaga agttgtatta gattatgggc taaatagaca
     1261 gtcattttta atttatgcaa taaataacgg aaatttgcca agcaataaaa agaacataat
     1321 tattgaaaca tatgcctatc tggagggggg atctaaaaaa ctaattaagt catgtaatat
     1381 agagtatcaa ttatcagtag gagaaattaa atgtgttttc gaagaagatt tagaacttga
     1441 cttaaaaaaa atttttgcca gcattcatga ttatataggt tt
//
LOCUS       DACIWG010000138         1457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.149, whole genome shotgun sequence.
ACCESSION   DACIWG010000138 DACIWG010000000
VERSION     DACIWG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.149"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            109..1041
                     /locus_tag="IYF54_002698"
     CDS             109..1041
                     /locus_tag="IYF54_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ9832953.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IYF54_002699"
     CDS             1113..>1457
                     /locus_tag="IYF54_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832954.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACIWG010000139         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.150, whole genome shotgun sequence.
ACCESSION   DACIWG010000139 DACIWG010000000
VERSION     DACIWG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.150"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(150..1337)
                     /locus_tag="IYF54_002700"
     CDS             complement(150..1337)
                     /locus_tag="IYF54_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ9832955.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACIWG010000140         1423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.151, whole genome shotgun sequence.
ACCESSION   DACIWG010000140 DACIWG010000000
VERSION     DACIWG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.151"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            98..1390
                     /locus_tag="IYF54_002701"
     CDS             98..1390
                     /locus_tag="IYF54_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9832956.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DACIWG010000141         1415 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.152, whole genome shotgun sequence.
ACCESSION   DACIWG010000141 DACIWG010000000
VERSION     DACIWG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.152"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            94..1389
                     /locus_tag="IYF54_002702"
     CDS             94..1389
                     /locus_tag="IYF54_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ9832957.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          450 a          279 c          265 g          421 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa aacattttta
     1381 accaaataaa cagaacagaa agaggttttc ttctt
//
LOCUS       DACIWG010000142         1381 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.153, whole genome shotgun sequence.
ACCESSION   DACIWG010000142 DACIWG010000000
VERSION     DACIWG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.153"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            82..1377
                     /locus_tag="IYF54_002703"
     CDS             82..1377
                     /locus_tag="IYF54_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ9832958.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIKVR"
BASE COUNT          474 a          257 c          261 g          389 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaaagt aagataacaa
     1381 a
//
LOCUS       DACIWG010000143         1364 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.154, whole genome shotgun sequence.
ACCESSION   DACIWG010000143 DACIWG010000000
VERSION     DACIWG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.154"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            82..>1364
                     /locus_tag="IYF54_002704"
     CDS             82..>1364
                     /locus_tag="IYF54_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ9832959.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DACIWG010000144         1352 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.155, whole genome shotgun sequence.
ACCESSION   DACIWG010000144 DACIWG010000000
VERSION     DACIWG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.155"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            11..1350
                     /locus_tag="IYF54_002705"
     CDS             join(11..452,452..1350)
                     /locus_tag="IYF54_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ9832960.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACIWG010000145         1343 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.156, whole genome shotgun sequence.
ACCESSION   DACIWG010000145 DACIWG010000000
VERSION     DACIWG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.156"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(69..1208)
                     /locus_tag="IYF54_002706"
     CDS             complement(69..1208)
                     /locus_tag="IYF54_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ9832961.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          351 a          289 c          249 g          454 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt att
//
LOCUS       DACIWG010000146         1195 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.157, whole genome shotgun sequence.
ACCESSION   DACIWG010000146 DACIWG010000000
VERSION     DACIWG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.157"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..1076
                     /locus_tag="IYF54_002707"
     CDS             <1..1076
                     /locus_tag="IYF54_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ9832962.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACIWG010000147         1190 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.158, whole genome shotgun sequence.
ACCESSION   DACIWG010000147 DACIWG010000000
VERSION     DACIWG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.158"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            141..398
                     /locus_tag="IYF54_002708"
     CDS             141..398
                     /locus_tag="IYF54_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832963.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IYF54_002709"
     CDS             complement(630..1043)
                     /locus_tag="IYF54_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ9832964.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          454 a          151 c          148 g          437 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta
//
LOCUS       DACIWG010000148         1155 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.159, whole genome shotgun sequence.
ACCESSION   DACIWG010000148 DACIWG010000000
VERSION     DACIWG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.159"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..128
                     /locus_tag="IYF54_002710"
     CDS             <1..128
                     /locus_tag="IYF54_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ9832965.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          438 a          151 c          164 g          402 t
ORIGIN      
        1 tgaacaattt aatagaacaa gaccatcgac ctattaaacg acgaaataaa ttttatcaaa
       61 gtctccgtac agcctcttcc acgattaagg gcatggagac cattcgagga atatataaaa
      121 agaactgaag aaatggaacg ctcttcggct tttcggtgtc tactgaaatc aaggtattaa
      181 tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacagaac ccattagcaa tatataaaag aaggtatcaa gagtcacagt gaatttattc
      301 cgaaaagtga aaagagcaaa tataaaattt aataaaaata agaactaaat aggaaaacgt
      361 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
      421 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
      481 tctatttaac tcattagaca ttctatgctc atttttctcc aaatttgttt caactctttc
      541 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
      601 atgatatata tctacagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
      661 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
      721 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
      781 tattgattgc aaaattatgt gacaaaaaga actagtagaa gaaggaataa tggcttgata
      841 ataaagtact acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac
      901 tatattttag gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa
      961 tttaaaggta ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt
     1021 aataagagtt tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag
     1081 tgatataatg aaatatctac acgacaaaat tatgatcttg cgtaaagtta taaaatatta
     1141 ataaaaatat tttat
//
LOCUS       DACIWG010000149         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.160, whole genome shotgun sequence.
ACCESSION   DACIWG010000149 DACIWG010000000
VERSION     DACIWG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.160"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            6..980
                     /locus_tag="IYF54_002711"
     CDS             6..980
                     /locus_tag="IYF54_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ9832966.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACIWG010000150         1059 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.161, whole genome shotgun sequence.
ACCESSION   DACIWG010000150 DACIWG010000000
VERSION     DACIWG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.161"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(37..993)
                     /locus_tag="IYF54_002712"
     CDS             complement(37..993)
                     /locus_tag="IYF54_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832967.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DACIWG010000151         1048 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.162, whole genome shotgun sequence.
ACCESSION   DACIWG010000151 DACIWG010000000
VERSION     DACIWG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.162"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..468)
                     /locus_tag="IYF54_002713"
     CDS             complement(<1..468)
                     /locus_tag="IYF54_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ9832968.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ"
BASE COUNT          319 a          225 c          164 g          340 t
ORIGIN      
        1 ttgcgtaata aaatcatcag gcacatcgat aatgtcgcaa gcaatatcag cacgtgagaa
       61 atgtggttct aaaaataaat catggataaa gttcttcatc gaacccgcca aaaattggct
      121 aattttattt gggttaaagt caattcgccc ttgcccttct tgatatttca aaatttcaat
      181 gtaagcaata ttctcatcat gcttatccac aatttgccaa ccatttagac caacagattt
      241 aacataacca ttattttcat tcagtcgcat taactgttca aagtctaaaa tcaatacatc
      301 attttgcgtg tgataataaa tattctcttt gagttttcca acaatggtaa ttcggtcaat
      361 tgaccactgt attttaaggg gggtggtcaa agtttgcaca cttaaccgcc tgttagatag
      421 ggctgttaaa ttttcccact catttatgat cgtgccacgt ttactcaaat tttgttcctc
      481 ctcaaagaaa aagagacttt tggaaagaat ttccagagtt cacgtaaccc taaaaattca
      541 tatccaaaaa tctctagctt gaagaaacct aaaaatattc gtataatgaa cctgagttac
      601 ggagctaggt gaagtagttt ccgtaaatag tcgagagtgt tagcgcactg ctcggctttt
      661 attttgctct tttttctcgt tttctttgat tatagcactc ctcctaaaaa aataaaaaga
      721 cctcagggaa tttcattccc cgaacccctt gagcctcctc ctgccgaaat ggaaagggcg
      781 cacccgctgt ttatttcatt cagctatcgg ctttcacaat ctaacacgat cgttccgcta
      841 ccagtcgcct tacaggctcc attttcgctc ctctaacgtg ttttaacgat tgtgccgata
      901 actaaacgaa ataaacgcta aaaccgctca gaacgcaaaa tatgccattt tagagctatt
      961 gtagaaaatg tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa
     1021 atctgctaat tattttctac cagacaaa
//
LOCUS       DACIWG010000152         1047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.163, whole genome shotgun sequence.
ACCESSION   DACIWG010000152 DACIWG010000000
VERSION     DACIWG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.163"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..203
                     /locus_tag="IYF54_002714"
     CDS             <1..203
                     /locus_tag="IYF54_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832969.1"
                     /translation="IYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQK
                     FLNRSHKGFQQVTDTLVSMFTE"
     gene            256..1038
                     /locus_tag="IYF54_002715"
                     /pseudo
     CDS             256..1038
                     /locus_tag="IYF54_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          438 a          155 c          137 g          317 t
ORIGIN      
        1 ccatttactc aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta
       61 gaaaagagca gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata
      121 catacaatca aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat
      181 tagtttcaat gtttactgag taactaatta ttttgcagga ggacaattta tttacacaaa
      241 attattgacg ctccccaaga tatgctagat tactactatt ttgaaaaaaa tttatcatat
      301 aattatgtaa tacatttaaa aacttttaca ggaatgattt ttaaattcgc caaccgtcga
      361 tatggattaa aatataatcc cgtaaatggg gtttctttag caagaaaacc taagacaaac
      421 gaagaaataa aaaaagaaaa agatggttat ttatcaaaag aagaagttca aatgattgct
      481 agtaaacaaa aaagttcaca tcaacagtca cgttattctt taattactaa atttttgttt
      541 ttaactggac tacgttatgg tgaattgata tctttgactg aatctgatta tgatggtaat
      601 aaaattacgg tttctggtac ttatgattac gagttaaaaa ttaaaacaac aactaaaaat
      661 acaggttctt atagaaccat tgaattatca agtaatgcta aagaaatcat agatcaatta
      721 attgaagaaa acaaattaat taagaataat aaagataaat acattttcat ctctaaaaat
      781 ggtaacccga tatctattca agcatataat caatctttaa gagcagtatc caaagaatta
      841 aacatagata aaaaagtttc ttctcacatg ttaaggcatt cccacatttc tctacttaca
      901 gaattaggaa tcccattaaa agccattatg gatcgtgtag gacatgaaga ttcaaaaaca
      961 acattaaaga tttatactca tacaactaaa aatatgcaaa atcaactagt agaaaaacta
     1021 ggaaaaatag aaatttaaat tgcccct
//
LOCUS       DACIWG010000153         1032 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.164, whole genome shotgun sequence.
ACCESSION   DACIWG010000153 DACIWG010000000
VERSION     DACIWG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.164"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            79..>1032
                     /locus_tag="IYF54_002716"
     CDS             79..>1032
                     /locus_tag="IYF54_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ9832970.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYD"
BASE COUNT          381 a          192 c          176 g          283 t
ORIGIN      
        1 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
       61 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      121 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      181 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      241 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      301 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      361 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      421 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      481 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      541 gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg tcaaaccgta
      601 tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa agaagtactg
      661 agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct acaggatatt
      721 aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa aggcatgaaa
      781 gatactatcc atcaaattta tcctaaagca aaatatcagc attgttgtat ccatgtatct
      841 cgtaacatcg ctcataaagt acgtgtcaaa gaccgaaaag aaatctgtga tgactttaag
      901 gctgtttatc aagctaactc aaaagaagaa gcgaatacct tcttatccgg catgattgag
      961 aaatggaaga aaaactatcc taaagtgacg cagtcactca tagaaaacca agacttatta
     1021 actttttatg at
//
LOCUS       DACIWG010000154          915 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.165, whole genome shotgun sequence.
ACCESSION   DACIWG010000154 DACIWG010000000
VERSION     DACIWG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.165"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(719..>915)
                     /locus_tag="IYF54_002717"
     CDS             complement(719..>915)
                     /locus_tag="IYF54_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ9832971.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGITA"
BASE COUNT          285 a          177 c          139 g          314 t
ORIGIN      
        1 atttgatact gccatctaaa ttataaacaa tttatagcga taataccatt gttatctctt
       61 tcacattcct atgctttgct gtatccgtca ctgtttccat gcaactttta tgatgtcttg
      121 ataataaatg actactctaa gaagaagcgc acccacgatt aaactgaaaa cgagccgtaa
      181 cactaaggaa aaatcttttc caatatcttc aactcacttg agaaggcact tacccttcaa
      241 ttccgatttt cacagagata ttgtctagca gaaataaaaa tggataggtc gcttttatcg
      301 tataaggtac cgtatctcca aacacttctt tttaccctaa tgatgaattt tgtatcatat
      361 actagccttg aaattgttta tttgagtaat ctttgagctc tgcaactgct tcagctgtca
      421 atccactttt atgttcaatc tgataattga cttgttagtg ataagtagtg attgtttgaa
      481 ttcaatatcc gaagacaaca acactcaaaa aaacgttacg accttgttga acatcatgat
      541 gttcaacaag ctatcttgct atacaaggat gtttttgaaa cgaaaacaat aatttgacac
      601 taatccattt aagaatgctg cttgagaaat ttttatcaaa tgacactact caaattccta
      661 aaatggtttt gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc caaatatctt
      721 aggctgttat tcccattaat accttgattt cagtagacac cgaaaagccg aagagcgttc
      781 catttcttcg gttcttttta tatattcctc gaatggtctc catgccctta atcgtggaag
      841 aagctgtacg gagactttga taaaatttat ttcgtcgttt aataggtcga tggtcttgtt
      901 ctattaaatt gttaa
//
LOCUS       DACIWG010000155          898 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.166, whole genome shotgun sequence.
ACCESSION   DACIWG010000155 DACIWG010000000
VERSION     DACIWG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.166"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            161..358
                     /locus_tag="IYF54_002718"
     CDS             161..358
                     /locus_tag="IYF54_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ9832972.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            360..671
                     /locus_tag="IYF54_002719"
     CDS             360..671
                     /locus_tag="IYF54_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ9832973.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            775..>898
                     /locus_tag="IYF54_002720"
     CDS             775..>898
                     /locus_tag="IYF54_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ9832974.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
BASE COUNT          353 a           88 c          165 g          292 t
ORIGIN      
        1 gtaaaaacat tgtatagcat tttacacagg agtctggact tgactgatta aattcttatt
       61 caggaattat tttgctacgt ttcaagaagg aattgttttt tgaaatttaa tgaatgttag
      121 aataatagtc taaaaacatt aatttagggg tgattagatt atgaaacatt taaaaatttt
      181 gtctattaaa gagacacaac ttatctatgg gggtaccact catagtggaa aatattatgg
      241 aaatggagtg tattgcacta aaaataaatg tacggtcgat tgggccaagg caactacttg
      301 tattgcagga atgtctatag gtggtttttt aggtggagca attccaggga agtgctaaaa
      361 tgaaaaaaaa tgctaagcaa attgttcatg aattatataa tgatatatct ataagtaaag
      421 atcctaaata ttctgatatt cttgaggttt tacaaaaggt atatttaaaa ttagaaaaac
      481 aaaaatatga attagatccc agtcctttaa taaatagatt ggtgaattat ctatatttta
      541 ctgcttatac taataaaata agattcactg aatatcaaga ggaattaata agaaatttga
      601 gtgaaattgg aagaactgct ggaataaatg gtttatatcg agcagattat ggagataaat
      661 ctcaatttta atgatactta taatattaaa tatgtctcaa aattttttga ttagaaaaat
      721 aggtatattg aatcagtgac ataaaagtat ttatagtttg tagggggaat cgtaatggaa
      781 gaaaaaaata gactaaatgc taaacaatgt tcagatcaag agcttaaaaa aattaaagga
      841 ggtgctggaa caaaacctca aggaaaaccg gcatcaaatt tagtggaatg tgtttttt
//
LOCUS       DACIWG010000156          897 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.167, whole genome shotgun sequence.
ACCESSION   DACIWG010000156 DACIWG010000000
VERSION     DACIWG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.167"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..434)
                     /locus_tag="IYF54_002721"
     CDS             complement(<1..434)
                     /locus_tag="IYF54_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9832975.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IYF54_002722"
     CDS             complement(451..855)
                     /locus_tag="IYF54_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ9832976.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          270 a          145 c          142 g          340 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttgtata gactgattgt aacatgg
//
LOCUS       DACIWG010000157          847 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.168, whole genome shotgun sequence.
ACCESSION   DACIWG010000157 DACIWG010000000
VERSION     DACIWG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.168"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            69..644
                     /locus_tag="IYF54_002723"
     CDS             69..644
                     /locus_tag="IYF54_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ9832977.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(713..>847)
                     /locus_tag="IYF54_002724"
     CDS             complement(713..>847)
                     /locus_tag="IYF54_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9832978.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          144 c          181 g          286 t
ORIGIN      
        1 cgttctttta aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg
       61 ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta
      121 ttgggatcaa ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg
      181 attctatcgg aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag
      241 gattgtccag cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc
      301 caatccaaat tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag
      361 ataaaaacag cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg
      421 tttcttcgat cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga
      481 tcgttcagct attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg
      541 cgggtacaga ctaccttgat cgcttgttgt ctatcgtcgt tgtttcatta gtcatcacta
      601 tgttgcccca acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat
      661 tagcaaaata atgctaatct ggctctgttt tttcatttta ttatttgtca aattaagcga
      721 gacaaaactt tccatttacg taactcaaaa aaacttctaa tggtgtttgg taatttagtg
      781 attttctagg gatatggttt cttttggacg caacggatga gatgaatcct tgattgactt
      841 ggttgaa
//
LOCUS       DACIWG010000158          844 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.169, whole genome shotgun sequence.
ACCESSION   DACIWG010000158 DACIWG010000000
VERSION     DACIWG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.169"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..501
                     /locus_tag="IYF54_002725"
     CDS             <1..501
                     /locus_tag="IYF54_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ9832979.1"
                     /translation="QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQE
                     TKRKIVPPEIKTWWRLELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIA
                     GLISDQNLWAGLARHTKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLG
                     LDVTEE"
BASE COUNT          272 a          132 c          211 g          229 t
ORIGIN      
        1 caatatcgtg tggttgaccc tgtttcattc aaaccaatct atgggcggtc tgggaagctg
       61 gaaacggcat attggggtag tcgtgccagt gagcgacaaa taaggatgta caacaaaaaa
      121 ttggaacaag aaaccaaacg gaaaattgta ccaccggaaa taaaaacttg gtggcgactg
      181 gaattgcagt tacggcgtgg aaaagctact gattggtatg cgatggttca tgaaagttta
      241 gacagtttcg caagtcctca ttttttgccg actgatacaa agcatactga tagaattatg
      301 attgcaggat tgatttcaga tcaaaattta tgggctggct tggctcgtca tacaaaatat
      361 aaatatcgaa atttgttgaa aaaagaaagc caaagtgatg agttagttaa tcatttacga
      421 gagacatttt cggaatcggc agatgttttg aaaaaagaat tggatacgtg gcttttgggg
      481 ttggacgtga cggaagaatg agtggatgaa tttttgtcca cttttttatt ttcagcacaa
      541 gtcggcatgg tgggtcgtgc gtttgcagca agagcgaaaa aacgctaacg aatccgcaaa
      601 tgccgacttg gataaaacag ttgcgcaaag agacttggct ttttataata aatttaggga
      661 tatcccagtg tcactctttg gggtaattcg ggggaaggag ttacccctca ctctggaaaa
      721 ttagagcgaa gcagacgaga ttatcccaat gaatatgacg gcttactcgg agtgataccg
      781 agtaaaaaag tcagataagt gagagtaaat ttacgtcaat gtttaagata agcgattatt
      841 ttgc
//
LOCUS       DACIWG010000159          836 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.170, whole genome shotgun sequence.
ACCESSION   DACIWG010000159 DACIWG010000000
VERSION     DACIWG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.170"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            9..>836
                     /locus_tag="IYF54_002726"
     CDS             9..>836
                     /locus_tag="IYF54_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ9832980.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     "
BASE COUNT          287 a          147 c          159 g          243 t
ORIGIN      
        1 gagtacaaat gacctatacc catcttacaa cggatgaact tgtaataata gagtcttatt
       61 tcaaaatgaa tcaatctgtt gctaaaactg cccattgctt gaatcgttca agacaaacga
      121 tccataaagt atatctgttc ttcaagcaag gaaaatcagc cttagaatat tatcaacagt
      181 ataagaaaaa caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag
      241 aatatattca aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag
      301 cgtttcctat tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg
      361 attcttctga cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg
      421 gaaaacaaac tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa
      481 atgagtttgg tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa
      541 ttaccttagt tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag
      601 cgattgatat tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca
      661 aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca
      721 atgatattgc catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga
      781 attctaacgg attgttacgt agagatggtt tattgaaatc tatggatttc aattca
//
LOCUS       DACIWG010000160          811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.171, whole genome shotgun sequence.
ACCESSION   DACIWG010000160 DACIWG010000000
VERSION     DACIWG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.171"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IYF54_002727"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IYF54_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ9832981.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACIWG010000161          789 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.172, whole genome shotgun sequence.
ACCESSION   DACIWG010000161 DACIWG010000000
VERSION     DACIWG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.172"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            22..714
                     /locus_tag="IYF54_002728"
     CDS             22..714
                     /locus_tag="IYF54_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ9832982.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          158 g          198 t
ORIGIN      
        1 aaaaaaagga ttttagtaac aattggtcta gcttttttgt tagctggttg taccaacggg
       61 aatgaatcga aaaaagaatc aacaaaagaa acagcgaaag caacagttgc aactactaca
      121 acaactagca cagagagcaa gtcaactgca gcaagtacag aaacagcaga tacctctact
      181 tcgtcagagt cttccaaaac gactgaaaca actcaaacat ctacttcaag ttcttctgta
      241 gaggcttcat cttcctcttc agaaagcagc gaacaagttg aacaaacact gtggaatgca
      301 gacaaatccg cacaattaga ggcatttatg accaaatggg gccaaacaat gggacaacaa
      361 tacaaaagtt atacgaatca aatgagtgtg gacttatatg gtcttaaagt ccctcaagct
      421 attttgaacg gggagtggaa gatggctatt ggtggagtgc ctgtttctgc agaatggtca
      481 gaaagtggga caggtcaagc agactatcaa atcacagccg tctattccga tgcagagaca
      541 gaaccatatc tgaaaaagca cgtctatcta ttcgggtttc agcaaaatca gccaaaagta
      601 ttggtgacac aacaaaatca ggggaatccc gataattatc tttacttcaa cgagacagcg
      661 aataatgaat tgaaaaatgg ctttaatcaa atcgtttatg gacaagcaaa ttaatgagat
      721 aaacaagggg ttgtgacatg tcttttatca caaccccttg ctcagtttat ttgttagtct
      781 tttattttt
//
LOCUS       DACIWG010000162          747 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.176, whole genome shotgun sequence.
ACCESSION   DACIWG010000162 DACIWG010000000
VERSION     DACIWG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.176"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..415)
                     /locus_tag="IYF54_002729"
     CDS             complement(<1..415)
                     /locus_tag="IYF54_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832983.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
     gene            complement(545..>747)
                     /locus_tag="IYF54_002730"
     CDS             complement(545..>747)
                     /locus_tag="IYF54_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832984.1"
                     /translation="IYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQK
                     FLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          262 a          114 c          109 g          262 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacata ctgggagcgt caataatttt gtgtaaataa attgtcctcc tgcaaaataa
      541 ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa cctttatggc
      601 ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag cgttctagtg
      661 attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc ttattgaaag
      721 actcgattag attggttgag taaatgg
//
LOCUS       DACIWG010000163          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.177, whole genome shotgun sequence.
ACCESSION   DACIWG010000163 DACIWG010000000
VERSION     DACIWG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.177"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            119..448
                     /locus_tag="IYF54_002731"
     CDS             119..448
                     /locus_tag="IYF54_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9832985.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..725
                     /locus_tag="IYF54_002732"
     CDS             438..725
                     /locus_tag="IYF54_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9832986.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKIIPVLLCGTHEKFYEQLKKLI"
BASE COUNT          283 a           80 c          138 g          230 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggtttaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaaga
      661 ttattccagt attgttatgt ggaacgcatg aaaaatttta tgaacagttg aaaaaattaa
      721 tctagtgatt t
//
LOCUS       DACIWG010000164          692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.178, whole genome shotgun sequence.
ACCESSION   DACIWG010000164 DACIWG010000000
VERSION     DACIWG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.178"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..605)
                     /locus_tag="IYF54_002733"
     CDS             complement(<1..605)
                     /locus_tag="IYF54_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832987.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          224 a          134 c           99 g          235 t
ORIGIN      
        1 atcaattcgt catatcttcc aaaatcaaca ttgtactcat aaccaagaag tgtcggaatc
       61 aggtcaattt caatttcttt ttcaattgaa cgtttaataa gtttcgtgga tacatacccg
      121 acaaaaaaga gaataatacc cacttcaaac aaatacatgt gtccatttac aaagtgaata
      181 aacgtcaata aaaaatcctt cactcctttt gttgttcttc cagattgttc aatttgtttt
      241 attaaagtcg ctagaattgc atttacaaca attaaatcga ataggctttt gaaaagtttt
      301 cctgctgtta atttttgcct ggtaaattgc atgactaatt gagtaaaaat agacaatagc
      361 atgacaaaaa tcaacgtcat tagcactgta aaaatgtctg atgctgcaat cctattggga
      421 tcatttatca agtaataagc cgttccaaat actccaatcg agtaaacggt taaatcaaga
      481 ataagtcgtg ccccgtaact acaaagtttt cctaatggcc ataattgcaa tacccaaaat
      541 ttctttcgtt tgctatttct tttcagagga agccaggtgt gttcaatcca gtcgtccgat
      601 ttcatttcga tttcttccct aaattctttc ctttaacgtt ttcctttgct actttagcag
      661 cgataggttg ctcaatgaac caatttttta ag
//
LOCUS       DACIWG010000165          656 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.179, whole genome shotgun sequence.
ACCESSION   DACIWG010000165 DACIWG010000000
VERSION     DACIWG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.179"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..>656
                     /locus_tag="IYF54_002734"
     CDS             <1..>656
                     /locus_tag="IYF54_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ9832988.1"
                     /translation="RYKDIACKLKESICKQMTIDGTFGEQFLEGIGDEEKTTYSVKNY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDPLYQNTMTFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEELICLADLDGSWW
                     WWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVLQIDPVDNLTFS
                     "
BASE COUNT          198 a          107 c          152 g          199 t
ORIGIN      
        1 agcgatacaa agacatcgcc tgtaaactaa aggaatcaat ttgtaaacaa atgaccatag
       61 atggtacttt tggggagcaa tttttggagg ggatcggtga tgaggagaaa acaacctatt
      121 cagtgaagaa ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg gaaaatcaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcctttata tcagaacacc atgacctttt
      301 ctgctagttc tttcaatcca acttatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagtgacatg cacaaccatc
      421 aagccttcgt aacccgatta gaggagctga tttgtttggc agatttagac ggttcatggt
      481 ggtggtggcc gtatcgttta gaggctaaac aaggagaagt tgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggcctttttg tcagtataat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttct
//
LOCUS       DACIWG010000166          617 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.180, whole genome shotgun sequence.
ACCESSION   DACIWG010000166 DACIWG010000000
VERSION     DACIWG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.180"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..614)
                     /locus_tag="IYF54_002735"
     CDS             complement(<1..614)
                     /locus_tag="IYF54_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ9832989.1"
                     /translation="MPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHAN
                     WHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTV
                     TSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVH
                     AFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DACIWG010000167          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.183, whole genome shotgun sequence.
ACCESSION   DACIWG010000167 DACIWG010000000
VERSION     DACIWG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.183"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(5..90)
                     /locus_tag="IYF54_002736"
     tRNA            complement(5..90)
                     /locus_tag="IYF54_002736"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IYF54_002737"
     tRNA            complement(107..178)
                     /locus_tag="IYF54_002737"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IYF54_002738"
     tRNA            complement(190..262)
                     /locus_tag="IYF54_002738"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IYF54_002739"
     tRNA            complement(280..361)
                     /locus_tag="IYF54_002739"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IYF54_002740"
     tRNA            complement(369..441)
                     /locus_tag="IYF54_002740"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IYF54_002741"
     tRNA            complement(445..517)
                     /locus_tag="IYF54_002741"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACIWG010000168          442 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.184, whole genome shotgun sequence.
ACCESSION   DACIWG010000168 DACIWG010000000
VERSION     DACIWG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.184"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..>442
                     /locus_tag="IYF54_002742"
     CDS             <1..>442
                     /locus_tag="IYF54_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832990.1"
                     /translation="FVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKVREENRV
                     LSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVISDAHKG
                     LVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIF"
BASE COUNT          159 a           83 c           80 g          120 t
ORIGIN      
        1 cttcgtttct agcttaacag aacagctaga acctatggtt aacgagtggc agaatcgttt
       61 attatcagaa aaaaattatc cttacttaat gaccgatgta ctctatataa aagtacgaga
      121 agaaaatcga gtactctcaa aaagctgtca tatagcgatt ggaataacca aagatggcga
      181 ccgtgaaatt atcggcttca tgattcaaag tggcgaaagc gaagagacct ggacaacatt
      241 ttttgaatac ctaaaagaac gcggtttaca aggtacggaa ctcgttattt ctgatgcgca
      301 caaaggatta gtctctgcca ttagaaaatc cttcaccaac gtaagttggc aaagatgcca
      361 agttcacttc ctaagaaata tctttaccac cattcctaaa aaaaattcaa aatctttcag
      421 agaagctgtt aaaggaattt tt
//
LOCUS       DACIWG010000169          422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.denovo.186, whole genome shotgun sequence.
ACCESSION   DACIWG010000169 DACIWG010000000
VERSION     DACIWG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.denovo.186"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            62..>422
                     /locus_tag="IYF54_002743"
     CDS             62..>422
                     /locus_tag="IYF54_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ9832991.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKV"
BASE COUNT          161 a           78 c           75 g          108 t
ORIGIN      
        1 taacagtcct tttacggtac aatgttttta acgacaaaaa catacccagg aggactttta
       61 catgacccaa gtacatttta cactgaaaag cgaagagatt caaagcatta ttgaatattc
      121 tgtaaaggat gacgtttcta aaaatatttt aacaacggta tttaatcaac taatggaaaa
      181 tcaacgaaca gaatatattc aagcaaaaga atatgaacga acagaaaacc gacaaagtca
      241 acgaaatggc tattatgagc gcagctttac gacacgtgta ggcacgctag aattaaaagt
      301 acccagaaca cgtgatggcc atttttcacc cacagtgttt gaacgttatc aacgaaacga
      361 aaaagccctc atggcttcaa tgttggaaat gtatgtatca ggcgtttcaa ctcgtaaagt
      421 at
//
LOCUS       DACIWG010000170        22694 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.041, whole genome shotgun sequence.
ACCESSION   DACIWG010000170 DACIWG010000000
VERSION     DACIWG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.041"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..151)
                     /locus_tag="IYF54_002744"
     CDS             complement(<1..151)
                     /locus_tag="IYF54_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9832992.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSIN"
     gene            381..878
                     /gene="dfrG"
                     /locus_tag="IYF54_002745"
     CDS             381..878
                     /gene="dfrG"
                     /locus_tag="IYF54_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ9832993.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(950..2902)
                     /locus_tag="IYF54_002746"
     CDS             complement(950..2902)
                     /locus_tag="IYF54_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ9832994.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2909..3205)
                     /locus_tag="IYF54_002747"
     CDS             complement(2909..3205)
                     /locus_tag="IYF54_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9832995.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3380..4183)
                     /locus_tag="IYF54_002748"
     CDS             complement(3380..4183)
                     /locus_tag="IYF54_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ9832996.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(4199..4315)
                     /locus_tag="IYF54_002749"
     CDS             complement(4199..4315)
                     /locus_tag="IYF54_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ9832997.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4560..5489)
                     /locus_tag="IYF54_002750"
     CDS             complement(4560..5489)
                     /locus_tag="IYF54_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9832998.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5506..6528)
                     /locus_tag="IYF54_002751"
     CDS             complement(5506..6528)
                     /locus_tag="IYF54_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ9832999.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6525..8552)
                     /locus_tag="IYF54_002752"
     CDS             complement(6525..8552)
                     /locus_tag="IYF54_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833000.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8549..11002)
                     /locus_tag="IYF54_002753"
     CDS             complement(8549..11002)
                     /locus_tag="IYF54_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ9833001.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10986..11381)
                     /locus_tag="IYF54_002754"
     CDS             complement(10986..11381)
                     /locus_tag="IYF54_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9833002.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(11453..12091)
                     /locus_tag="IYF54_002755"
     CDS             complement(11453..12091)
                     /locus_tag="IYF54_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833003.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(12147..12641)
                     /locus_tag="IYF54_002756"
     CDS             complement(12147..12641)
                     /locus_tag="IYF54_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ9833004.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(12708..13487)
                     /locus_tag="IYF54_002757"
     CDS             complement(12708..13487)
                     /locus_tag="IYF54_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ9833005.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(13529..13750)
                     /locus_tag="IYF54_002758"
     CDS             complement(13529..13750)
                     /locus_tag="IYF54_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833006.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(13747..14037)
                     /locus_tag="IYF54_002759"
     CDS             complement(13747..14037)
                     /locus_tag="IYF54_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833007.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(14034..15218)
                     /locus_tag="IYF54_002760"
     CDS             complement(14034..15218)
                     /locus_tag="IYF54_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ9833008.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(15400..16803)
                     /locus_tag="IYF54_002761"
     CDS             complement(15400..16803)
                     /locus_tag="IYF54_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ9833009.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(16825..17598)
                     /locus_tag="IYF54_002762"
     CDS             complement(16825..17598)
                     /locus_tag="IYF54_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833010.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(17608..17985)
                     /locus_tag="IYF54_002763"
     CDS             complement(17608..17985)
                     /locus_tag="IYF54_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9833011.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(18006..18320)
                     /locus_tag="IYF54_002764"
     CDS             complement(18006..18320)
                     /locus_tag="IYF54_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ9833012.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(18520..20313)
                     /locus_tag="IYF54_002765"
     CDS             complement(18520..20313)
                     /locus_tag="IYF54_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ9833013.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(20310..22499)
                     /locus_tag="IYF54_002766"
     CDS             complement(20310..22499)
                     /locus_tag="IYF54_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ9833014.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
BASE COUNT         6838 a         4293 c         3644 g         7919 t
ORIGIN      
        1 catttattga agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg
       61 aatctcgtaa atgtagtact ccactataaa ggcgtatata tgcaagtaag tagttcagtt
      121 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
      181 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
      241 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
      301 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
      361 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
      421 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
      481 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
      541 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
      601 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
      661 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
      721 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
      781 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
      841 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
      901 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
      961 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1021 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     1081 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     1141 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     1201 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     1261 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     1321 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     1381 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     1441 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     1501 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     1561 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     1621 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     1681 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     1741 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     1801 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     1861 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     1921 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     1981 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     2041 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     2101 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     2161 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     2221 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     2281 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     2341 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     2401 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     2461 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     2521 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     2581 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     2641 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     2701 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     2761 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     2821 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     2881 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     2941 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3001 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     3061 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     3121 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     3181 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     3241 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     3301 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     3361 tactccaaaa ctgaactact taatatgcaa gacgttgtct tttttttgta tattcaattt
     3421 tgaaaacatt tccgcaaagt tcagacggac ctcgatgtgt tgatgaataa aatttattag
     3481 taataacttc tataaggtta tcaatcccta tattactttt tgcacttcca tgataaagag
     3541 ggaacagaga acaattctga aatcttatgc tttcctcttg ttcgagttcc aatgcttcta
     3601 atgatttacc ggacatatat ttctctaaaa ggtcatcgtt tccctctatt accgtatccc
     3661 attgttcaga ttcggtaaag ttcgtcacac acatattagg atacagttct accttctgtt
     3721 tgattacaat ttcggcagaa agtttctctt taatatcctg ataaaccgtt gataaatcaa
     3781 ttccattttg gtcaatctta ttgataaaaa agattgtggg aatccccatt ttcctaagtg
     3841 catgaaataa tatacgagtt tgtgcttgta cgccatcttt tgcagaaatc agtagaattg
     3901 ccccatctaa aactgataat gaacgatata cttctgctaa gaaatccata tgtcctggcg
     3961 tgtctatgat gttcaccttc gtattttccc actgaaaaga ggttattcct gtctgaattg
     4021 taattcctct ctgacgttct aaaagcgtat tatccgtcct cgttgtacct ttgtccacgc
     4081 ttcctaattc tgtaatcgct ccactgttat ataataagct ttctgttaag gtagtttttc
     4141 ctgcatcaac atgagctaaa actccaatat taataatttt catgtgattt tcctccattc
     4201 aaaaacccaa aagggcataa aaatcccagt gataaatact tttatcactg ggatttttat
     4261 gcataaccat aggtatacaa agcatacaga tattctctgg atactttaga atcacatgat
     4321 aaaggtattc ttaaactggg tacaaaaaac taagccctcc taaaaaagga catctaatta
     4381 tttgttcccg ctatcaaatt gacagtttat ttaagaatac cttgccgcat atttattaac
     4441 tccttttaaa tagtcactta aataatagca cgtaagagca tatttgtaaa ggaatctcca
     4501 attttttatc aaaaagagta catgattaca aagtatctgt aatcatgtac caatatttgt
     4561 tattttacaa tcttccaatt atcttgtttt tctaatatca actcaaattg cgagatttgg
     4621 gttgcctttg tttcttgatc taaatatttc acagcgacat tcacgattac ttggttatct
     4681 tttctggtaa agataggatt caccaattcc tcgaaaacat attccttgtt aatcataggt
     4741 aacacatgat tacttacata gtaagtcaat tctttttctg tggcggtagg atagagctgg
     4801 aagaacgttt ctaaaaagct agtgatttcg tccatcgttt ctgtatctac tgtatggtca
     4861 ctttctagct gtttgggttg gtagtccgat ttttgaggtt tcttactcat tgtcggattt
     4921 ttgataataa ccatattatc tgattcatct atatggacaa ccacatgaaa gctagacgat
     4981 atagtttcct tgtctttgtc ttcggtaata acttgttcaa cagaaaacag tacctcaaag
     5041 gtgttctcat tcacttgaga aacctgccac acctgtatat cgttcacact agaactagta
     5101 ggaatatcct tacgaatcat ttcttcattt aatacctgca attcttccgt caaataatgg
     5161 gtcaattttt catttcgttt atcaatggct tcttgggatt gttgccacga aaagtagtct
     5221 ttcgcaaagg atttcacaaa actttctact tggttggtat cgacaatctg ctgtttaatg
     5281 atttctgttt cacggacagt atgagtgtca atggcagtaa aatttttata gattccaaat
     5341 ccgacactac caatcaataa aacccataaa acaaaggtta atttcttatg cgtgccaact
     5401 ttaatcatcg gaacttttct ttgcttgctc ggttttttag atttctttgg tttctccttc
     5461 ttttcttttc gctcgatttt tataatcatt tgattcattc ctttcttagt tatgaataac
     5521 acgtctagcc ccaatcagat ggtcttgcca ataacgactg cttaagtcgc cataaccgat
     5581 tgggtcgcct gcatgataaa agcgatttcc ctctaaatat atcgccacat gagtcacata
     5641 tgtgcctgca ttataggttg aatgaaagaa gataaggtcg cctgcttggg cttcttccat
     5701 agagatttct tgcgtagcgt catactgatc ttgggcgaca cgagggagag aaatgccagc
     5761 tttgtcatac acccattgta ttaagccact acaatcaaaa gaggtggtag gcgacgcccc
     5821 tccaaagaca tagggaaacc cttcgtattt caaggcttcg tccataatga cttgcacggt
     5881 ttcatcatca aattctgttg gggaagtgtc ggttaggtat tgtgaaacca actgaacata
     5941 aaactgattt ccatagttat atcgccaacc accattcaca ggtatggcta taggattggg
     6001 atagtctgct ttttgaccac ccgacttctc tttagaaaac tgttcggcta attcataggt
     6061 atattttttc ccatgactcc tcacataatt taaaaagcca ccgccataat tataagattg
     6121 gataacagaa tctatatcca ctccttgttg ttctgcactg gttaataatt cactgaaata
     6181 tttaacccct tgtttgatgg actcttcggt actcaaactg ttgggcggta aacccaaaga
     6241 ttcggaactt tgcataacat cttctgctgt accgcccgat tccacttgca taatggctaa
     6301 gatataagaa acatagtctt caatcccata ttccttacca tatttttcaa tcaacggacg
     6361 gtgtgccaac acttctggtg aaacggtcac accgccttgt ggaatagaag aatccttgtt
     6421 gttttcgcca ctgtccgtat catctgcaaa gaaaatgact aggaataata agagaaaaaa
     6481 gaagagagag aagaccacac tacaaatcac tgttattttt agcttcatgt ttgttgtcct
     6541 cgcttatccg ttcttttatt tagcttcctc tgattgaatt gcttcggtgg tgtcttttgt
     6601 ttttgctctt ttttcaatgg tcgctgatga ttttcacgtt tagtgttttg tgacaaaaca
     6661 ggcgtgacgg tttgctgacg cttgcgaaca tgctgctggt tattctgaac aggagatgat
     6721 gggttttctt ttcgtctttt ttcttttgct ttatccagct ccaaacgctt ttgagcaatg
     6781 ttctttcgtt tttgctcctg ctggttcata cgctgttgtt tgcgttgagc tttggtatct
     6841 gccatagatt gtttaaagtc tgttacatta tgagtgacat tctcttttcc ttgatgaagg
     6901 gcatatttga catgggttgg tgtcgcctgt atctgttgtt ttgcctgctt tcttttattg
     6961 actagacgat ttttcgtgtc caatacatgg gctgtctttt cacctaatcg ttgcccgata
     7021 ccgccttgtt gctttttcac attggaaagt gctgtttctt tctttggtgt agtccgttca
     7081 ttttgacgag tatggttagc tttattggtt tgattctgct ttgttgtcat tgtcttttgc
     7141 ttcgttgacc ctgcttttcc acgaaaaatc ttactcatac tgcgttgaaa tcttctggtt
     7201 tgtcgattca tcatttgacg aggtctgcga aacacttgtc tgcccatgtt ttgagagtcg
     7261 ttgctttgta aagtgaatag gctcattaaa tcgcccagtt taaaataaat ccctgcaaag
     7321 gtcacaattt gtaaaaacat aatcatgaaa aagggagaac ttgctgacag cgaataaagc
     7381 atagtggaaa tactaaaggc aacggtaata atcaaggtaa taccagccct tgtcataatc
     7441 acattaaata acttggtaat ggcatgcctg cccatgccat caaaactcgg caacatactt
     7501 aataggaaac tgattggcaa aaacatagcg aaaataataa atagcacttg cgaaaaaatc
     7561 atgattcctg ttagaaggaa aacaaaaatc gagataccaa tattgaatac aaacaaaaag
     7621 aagactgttc ctaatctgga aatcgtcttc ggtagggtta aataggtatt gtcatgatct
     7681 tcaatttctt caatcactac attttctcta tcttcaccat tgtttgtatc tggactggta
     7741 gacaataaac tttccacacg ttcttcccct aacgcttcta catcagtgga gccatattga
     7801 agcaatagcc atggttgctt tacttgaaca gagaataagc tgtcacgtat catgtccaca
     7861 ctgtctttcc cttgactatc cgtgttagga agaacaattt ttgtcccaat attcagactg
     7921 gcggtactga catctttgga aaaatcattg attttcgcaa tataatcggg tgcatacgca
     7981 ataaaggaag ccgatagaat aaacaccacg acaaagttta aaacagctcg tatcactttc
     8041 gtcgtttctc gcttaaccaa tcctgtatag gcaacataga ttccaacaat gagaataaaa
     8101 agaaggagga aaccgacata aaaccctgaa ctggaaaagc cattcggtgt aatgcctgca
     8161 atcgtctgca tgttttcgcc aattgcttgg gatgtttgac taatgaaatc taaggcatag
     8221 gcttctttca ccaaataacc tgtggcgttc gatagataaa gagaaattgt ccaaataaaa
     8281 ttagtaatgg catacaatcc atacatcacc gattgtccta tgccatcact ccaattccat
     8341 ggaagccagc cccagctatt gtccacataa taatcaagct gatattgatt caaaccatac
     8401 ttggagtatt cattgcttgt atcaatggtt tcatcaacta agcctgtcgc ttgtactgca
     8461 ttgcccagcg tgcctaataa aagtaaaata acgcccacac aaaggagcgt tatccaacta
     8521 tagtgaagaa atttcttttg tttttgtgtc attcttccac ctcatttcgc acaggcggtc
     8581 tggtatcaaa ggcatgaagc aactcttcaa aaatcgggtg aaactgcata acccctacac
     8641 gaccatagat gtctgaaact aaacattgtc cattttctaa ttcacgtaag cgtttctgat
     8701 tgttttcatc ttccttgtcc acaccaaaaa attctaaggt ctttttgatt tccactaggt
     8761 cagtggaacg aaaggcaaat ttcaagccaa tattattctt catcttttca tctagtaaat
     8821 cgtccgcgtt ttgagtaacg aaatagaccc ctgcattcat ggcacgtcct gctcgaacca
     8881 atcgcataga tagagctttt ccttgtgcca cttgtaaaaa actccaagcc tcatcaaggt
     8941 caacaatttt aaaaatggaa cggtcagagt ggataaagtc taaagcaaag gtactaatga
     9001 caatcagcat tgccacactt aataattcca tggtagtgta ttcttcaaaa gagctattgg
     9061 aatcgggtaa gaccaaatct gccacttgaa taatattgag ttgtttttcc aagctaatgg
     9121 attgggtgac actgccatca ctgaataaca gatgggcaaa atcataatcg acaaagcttt
     9181 cgatatggtc agcaatacta ttgctgatgg gcgtatcttc ttttcgcaat tcatcaatca
     9241 ccaataacaa ccctcgttgt ttgctttgag ttacactccg aatagcttta cgcaggacag
     9301 gaaacttctc gctatctcgg ctggaaatac ctgttaagaa cgtgagaata tcaatggcaa
     9361 ggctttctga atctttcggg agttttaaaa tcacataagg gtcaagtaac cctttattgt
     9421 cgttctcact cgtgagatta acaatattga tttcatgggc aatatcaggt aaggtttctt
     9481 tccattcacc acgttcagct tttgggtcaa caatggttgc ctgtccgcca aagagtactg
     9541 aataatagac cagcatattg ttagagaatg attttccgcc acctaatgaa ccaataaaag
     9601 cggacgctaa tgcatttgtt acagagcctt taattccttg acttgcaagg ctaggttgga
     9661 gataaatatt tctgcctgta tcgagattgt aaccgacgta aataccagaa ttttcaccaa
     9721 tcatttgtgt agcaccgaaa ccaagaccag ccaaaaaatc cgacgtgaca tattgaatat
     9781 agtcattgat atatcgcttg cttgagggaa taaattcatt atgcaagcct agcatatcgc
     9841 caaaaggtcg tactaatttg atagacagat catcataaaa atcttttact tcatcacaac
     9901 gttgtttcag ctcgtccaaa ttctttgctg atacccgtac cacatagctt agtttgtaca
     9961 tagattcttt ggtttggtct aaattacttt ctaattcatc gacagattct aaagcgtcca
    10021 ccacattagt actggtttcg ttgtcgcttt cccatgcgtg gctatctaaa tctttcagtt
    10081 ctttcttttt attgcgaacg gtagataagg cttttttatt ggcgacaatt tcaacattca
    10141 tactggtatc aacggggaaa gtaaactgct gttgctggta ataaaaaatt tcactattag
    10201 ggaaatccaa ttcaccaaca atactattaa ttgtaaaata cgccacataa gtagtggtat
    10261 cttcctgttc aatccgtaaa tagcgttgat tttcttcgat taaacagcga gtgggtttga
    10321 gaatatcgta tttctttacc aacacttttt tatcttcgta tcgtttcggt aaatggtagt
    10381 cgtagtcatc aaaagggaca cctgtttgcc cgtaaagatg ttcaataata taaccaaaat
    10441 catctttctc caatttacga atctgaaagc gtctggataa tttactttct aacaactgtt
    10501 ccatcttaga aaagcgttcg atttccgcca taggcataga aacaaaatcg cccattaatt
    10561 tgtgattcac accatgaaag aattccgcaa atgctttttg aatttctttc ccaacatttt
    10621 ttacggatac ttcctgttca ttgaggtaga gtttaaaacc aataaaaaag cgatagtcta
    10681 cttgattgtc accaatcata gaaattaacg cttcggtctg ttcatctatt ttttcgtaag
    10741 ctatctcttt taatcgtcca gtgatttctt ttttcgattg ttcctgtgtg gctcgaatac
    10801 ttgattcggt tgctagttgc agagcatgaa tcttgccatc acgattttgg gcaatcaatt
    10861 gacagaaatt atcatgaact tgaatttttt catcggaact taaaaaactg taattatagg
    10921 gaattaattc ataataagcg aatacttcgc catcatgatt aaatacgaga ttatcttcca
    10981 tatatttaat cggatatgcc atctagttgt tcactcctta caatcgtaat cgattcattc
    11041 attgtttctt tttttaatcg tatttgtttg ccagcgtagg ttactttagg gcgtagagca
    11101 taggtaatga tagactttag aaatccatag ggtttctttc cgtcaaaggt cttttggcac
    11161 atgaacccag tgagaacaat gggaatccct aagtatttga gtagtgctcc gtcaatcaga
    11221 gaaagtggtg gtaaactgcc aaagagtatg acgacaaaca aagaaacgat aaaccatgtc
    11281 atttgcgtaa aggtaattgg gaaagggagt tgtaaatcat taatcgcata aatcactttt
    11341 tctactgacc aaatactggt gtaacttctt atttttttca tgtcttcacg tcctttccgt
    11401 aaaaatagaa aaagaacaat caaaataggt gattgctctt tcaatgcttt aactattcaa
    11461 ttttattttt tgtccatttt gcaataatac cttcatcagt ttcttcatca tctggattca
    11521 ttgaatatct gaaacttaaa ttggtatctg accctaatcg ttcatataag ctgggtctat
    11581 acaatctcgt tttattgtca ttaaaaggac ttcgttttcc cagctcacca tttcccttta
    11641 aaggatttct tctaactgaa aaacaatatt tttttctggg atcttctgaa tcactttgac
    11701 ttaatgagaa acacatggct tctttttggt cttcattttt cttctcacta acttctgtcg
    11761 gaataaatcg agcaagagtt tggctaaatt gaaataaaac atctcccaag ttactactgt
    11821 attcgatacc aaaatattct cttattgttt tcgtgtctgt atataatttt accgaattag
    11881 catagacagc tagcatatct gaaaaatcgt tttcctttaa aaattctagt tttaacagag
    11941 catattctgg aactttttcc tctttttcaa atagcttaac aagtacaata taattttgct
    12001 gtttgtaacg aaagtaaaag gaatctatca cccagccttt atctttcatg tctagtttta
    12061 atagtttaag attttcaaat acatttacca taatttatac ctctccgtgc tatttattaa
    12121 tttaattata acacggattg ttaaacttac tgacaatatt caaatacccc atgagaggta
    12181 actaggtaat taccctctat ttccatatca cgtcccaaag attggtagtc aatataattt
    12241 tgtaaattgg aagggacttc accaagctgt cctgtttctt ccacgtaata acgggcgaca
    12301 tcttccatcg aatcacaatc actataacaa tgaatatcct ctttgttttc cagtaattct
    12361 tccaagctac taaaccacat gccttgaatt tctttgagtt cattataaat aggtgtacct
    12421 tcgatttctt gaatggcttc acatagacgg ttgatttcac taatcggcgt gtattcgtcc
    12481 acatcaaaag gaagctcaaa atcatgtatt gcatattctt cgtagtcctc atttaaaccg
    12541 attctttctg ccatttcttc ctcatcaatt ggtggagtaa accatgctcc gactaattca
    12601 ccttcattat accgtcctaa atttgccaca tagactttca tttccaattc ttatcacatc
    12661 ctttttataa aaatagtcat aaaaaaagta gcaatcagtt aatgacgcta cttatcaagg
    12721 tatccgctaa tataatctgt aactgtactt gaaataccta ttattagttt tgcttctctc
    12781 tttccaataa tatttgtatt agggtgtgca agtgaatgcc tatttcttgc ttcattaaga
    12841 gagctaatca ttccatttga tgaccttatc gttgtcttca ctatatcagc aagttcagta
    12901 ggttgaattt ctttttcaat taattgttgt atcctactat ataatttaga tagattttcg
    12961 tctctaggaa ctgtaatttc atattttttt agtatttcaa ttaaatatcc atgaaaagca
    13021 gtgtgaactc tgtcaaaggc acgttgatat tcaccattac tcataaaaga ctcagcgtct
    13081 tcaatagctt gtgaaaccac atcactaatt ttcaactgac taaagttaat tacttcgtaa
    13141 ttctctggaa gtggtactaa aatagtatcc actaatacat tatcaccttg tttatcatat
    13201 atggagcttg ctgtctgggt aatcaaatct ttatgacttt caaaaaagtc taaatcatca
    13261 aaaggaactc ttattttaag atatgtgcaa agcgtattcc agcccttttt atatccttta
    13321 taataagtaa aatcgtcagt ctcatctaaa gttacctctg atattttcat tttagaaaca
    13381 atctcgttac ctccttcata ataatttgaa gagctctttc tttctaatgt tttaattaaa
    13441 agagatttaa attgatcatt tgtaaacata ttcataaatc ctttcataac aaaatatata
    13501 cttcctattt tatcatgaaa aatatctgtt aagcaccaat gattcggtta aataagttta
    13561 ataacacatc tttcacgcca gctgcattga acacaagtcc gacggcgatt agagcaataa
    13621 tcaagaagcc aatgagtttt gaaaattctc gtttaaaacc taaaaacagt ccaatgacaa
    13681 caatcgctaa caataccaat gactgtgcat tatccaagaa ccagttgtac aaattctgcc
    13741 caaaattcat gtgattgtcc tcctttcctt aagatatttt tctcgataat agatatagct
    13801 ttttacacgc cttaataatt gaaaaggggt taaatgctct ccatagacgg ttactcgatt
    13861 ggtataatta ttataaaatc tcaccttgta aaaatcatag gtatagatgg tatcaccagc
    13921 ttgccgtttt ccctcaataa tgagagacgt atatttccct gtattttgta tatacgcttg
    13981 aaactcaatg ccacactgaa accctaactt ctttaattct tttgttaaaa ggctcatgta
    14041 atcaaatcct ccatggctag atgttgttgt tcaattaatt tctcatgttt ttctgtcagt
    14101 ttggcttcct gaatcatggt ggagataatg gttgtaccgt tgagaacatc taaaatgctt
    14161 gcgattttta atgtaggggc gacttgatga tgtaaccagt tcaatgttcg ttcaaaagaa
    14221 taaggctctg gctgtgtggt taggcgtaat gctccacgat ttttaccgat aaaccattcc
    14281 caacgttcat tagttttcca atcactccga cgtttagtgt cgtctttatc tgcaaagcgg
    14341 atatagcgat tgataatttc aaaggctgtt ttttccgcct gttgatgtgc caataaatct
    14401 ttcatggctt gggtggctcg gtcatttttt aaccgaattt caaaccgatt ctttacttct
    14461 gcgtcttcta agggaacatc atttttcacg tattgctcat aatctttctc atatatacag
    14521 aaatagactt cactttttaa tgagccaata taaagtgtgt ttcccatgta tggtttttca
    14581 tcacggtgaa ccaattcacc actgcgataa tttttaaagc ttcgaaagac ggaaatgcat
    14641 tcttcctgtt tacatttctt tgccaaatct ggaatattca aaatccctgt tttatcgttg
    14701 atagctaaat ccaagcgttt gaaaatacct ttatgttcca tgcaatcttg aaagaaatca
    14761 taccagctcc gatgttgagc caatagaaag ttttcaaatt gccgacagcc ttttcctttc
    14821 agttctaata aaacaccttt acttacatca tgagagacta ggacaacaat atcaccgaaa
    14881 cgatagtgtt cgggataaga ataatagcca aagtcttcgt gtagcatata atccatattc
    14941 aaacgtaaaa cctcaccgat aatttgagcc acatcattgg tagggaatcg aatccgtaca
    15001 tagtcgaata acatctccaa tgggttatcg ggattgaagc gttccaaagc gttcagtaaa
    15061 tcttctttca cctgttgagt agggactgcc ttgcctgttt caatatccga taggtaaggt
    15121 ctggtaatat gagaagcaac cgccagttta ttttgagaaa cgccataagc cagtcgcttt
    15181 tctttcaatt ccttaatcca aattttttcg ttttccattc gcaaacctcc aatctaaaaa
    15241 agatgtaaac tatgaccctt gagtttacac ctttcatatt ttccgcaatc ctttgtacca
    15301 tcaacattgg ttgatgagaa ttgcctgttt tcagttgtat ttgtgccccc ctgttagata
    15361 gagggggcta aaggattggc gtggctaacg ccacaccaat caaagcaagt ctgaacctgt
    15421 acctttcact tcgtgcggta caggctcaga ttgctttttt tgaatgactt ttcctatttc
    15481 tcctaagaaa tcgtaccttt ttggtacaag gggtgtataa aactcactaa tcacactttt
    15541 tcctgtatcc acgtaacctc ttcccttgat gggttttaag aaaaattgtt tttgtgtatc
    15601 gcttccgaac atcataccaa agcccatttc agacatacga cccaaagcca cacggaagtt
    15661 aaagttgtcc cgaatcccat cactaaaata ttttgcgtct ggacgttgac aggcaagaat
    15721 taaaaagaag cctgcttgtc gtcctaacat tacaatttgt ttgagtttat gaataacggt
    15781 ggtactttca cgacctagca tatccatcat tgccacatat tcatcaaaaa taagaaaatt
    15841 ggcagacaaa ccaagataag cataattttc tcctgtttta tagttagggt gttgtttcat
    15901 ggtttcactg cgatcaatca tatcttcgta aaattgattg atacatgcca acatatcttc
    15961 tttacggtaa tagacattat tcatgaccgc ccctaagtct gccaaatcag catttttagg
    16021 gtcaagaata taaagctttg catttgtatg caggagagat tcaatcaatg tgagaataaa
    16081 ataggatttt ccactaccag tgcccccagc aattaacatg tgagggagag aatcgaactc
    16141 ccaatagacc gattccatca atttgacacg tccatcttga gctaccacgt cttcaatgcc
    16201 aattcgattg ccaatagtat cataaagtag gacatactct acataagaat ctttcaacgt
    16261 tttttccact aattcacaat acaatcctgt ttctagtttg ttttctaggt gcaaaagctg
    16321 atcttgatat ttccctaagg tgatttctac atgaatatgg attagtccat ctttcattcg
    16381 ataatatatt ttaggaaaat ggcggattcg ttgtttggtt ttcttgctgg gtaaatcttt
    16441 aaagaatccc tctgattgag tttcttcggc ttcataccaa tgattttcta aaatcatacg
    16501 agctaacttt tgacggtgct ctaactgttt tatagaatct atacgatagt gataataaag
    16561 ccagctgata agtccgcata caaaaagagc agtgagaata ctgctccaaa agtatggcgt
    16621 atgaagttga tagaaaaatg tatctaactg aactgcttgc cagttcaccg attgtaacgc
    16681 tctccaatga aaagggagta ggacgactag aaaaactacc agcaatgaaa ttagtacaaa
    16741 atgatagacc aagtgcttat cgttggcttt gattcgctct cctctgtcat gaagcaatgg
    16801 cataaaaatc ctcctttcca cctatcaaaa taattgtgta ttgtagatac gagcctgttt
    16861 atcaaaagtt ggttgagaaa tggcttcata tttctgataa gcgattcgct tttcaaagcg
    16921 taaatttttt tctttcggat aataatgacg tcgatagaga gtgtgtccta acagtttttt
    16981 cgcttctttt gtcaacggat agagatagcg aaacatgcgt ccattgattt tctcaatccc
    17041 tttgtattcg caaaatgcct gggaaagcca atgctttttc tctacctgtt caaagcgagc
    17101 attttcctct aataaaaggc gagcacttcg agggtgtatc ttttcccatg actgcttatc
    17161 acgatagaca cttgtcttga aatacccgca gtaaaagaag ttggacgctt gatagacata
    17221 gccacatttt ccctcaattc catctgccag tgtatataaa aaatgacagt ccgtatgttc
    17281 tttcaaccac ttaattagag ctgaaagagc ttgactgcca aaataattgg tttgattcat
    17341 ttctggaaga aaacacatct ttccaatttc taaataatct gtggtttgaa gggaactgtc
    17401 gggaaagagt ttgcgtatgg tttgtagggg ttgcgtaccc cagcctaatt ccacaacgcc
    17461 taataatttt tcttctgaaa aaatccctag aaagtattta cacaatctag ggagtatctt
    17521 tgaataatgg tattgccgaa taaaagaaat ggcttcatcc ttcggaattt ctttcaaaat
    17581 caatggtgtg tttgtcataa gcaacaccta tttttcactt tcggacgctt cttttttata
    17641 tttctgatga ggttttgtct gtccttttaa tacaagatcg tctgctttca cgtaccaatc
    17701 aacgtctgcc cctcgaaaag tagccgtcgc tacggtatct gccacaggat tgattaattc
    17761 cacttctgcg ttgtagtcaa attctttcaa aggaacagaa gcggggatac tgacttgtat
    17821 catgcgtcct tgtccttttg attttaagtc ataggtacgt tctttaattt cttcggaaac
    17881 cgtaccatct tcattctgga tacgaacctc acgacgcaag gcggaaaatt tcaatgtgcc
    17941 aaatgtctgg tctttttcaa tcacaatacc ttctgctaat ctcatatttt ttcacctttc
    18001 ctttcttatg ctttcaccat atcgtctgcg tgcataatgt aattggtaaa tcctcgattg
    18061 ccaattttat aaccttctgc ggtaatatgt gggttgatga gtttgacttt ttcgtcggat
    18121 tcaaaatgtt tctcgccagc ttcggaggga agaatgacaa caatatcatc tgctcgttga
    18181 atcgttgagt ataaattgta actacgagag agaatggttg gtctgccatt tattcgacgt
    18241 tgttcaatgt tcccttcacc agcatattct aattgtccaa atgttttttc catattggga
    18301 atgacatatt taagttccat atttttacct atcctttctg tttttatgtt tgaatgaatc
    18361 ttatttgtgt gatggtttta aaggggggag agcgaccttt tgattcttga ttttttattt
    18421 tcataagttc acttcctttc aaaattgggt aaaaaaatag acgctccgta ctgagcgtcc
    18481 gattcctact atatttaaaa caacgactac ctaggtattt taaatattat aatcaaattc
    18541 tattttatct aataattgag tgggataaag caagtctcct ttactcgtaa aataacccac
    18601 ctctgagaaa agaaataaat tattttttgc tcttgaacaa attacgtata gtagttttct
    18661 cgctgaacta attcctttat cagaaacatt aaaaatttca ttccaatgag gaattttccc
    18721 gtttagaaga ttaatagcga taacagtatc atactcattt cctttaactt tatgtgctgt
    18781 agtaagtgtg ataccatctc gagatttaaa catcttctta aaactagata cactactaga
    18841 taatgaatta ttttttatcc gatcttctac ttcgttaata aaaaatagat agtgcttaaa
    18901 aatagacggg taatttttct cgtcaatatt aagattaaca aatatatgtt tggtaacaaa
    18961 tttgtataaa tcaacaccgt tgtcgtctcc actatacatt cttttcacac tgtttattag
    19021 ctgtaataaa tctaagtcaa taaaataatc tttaacttct attccaaatt cattttttaa
    19081 tataattaaa atatcgtttg caattagctt tcttcttcga accttttcac ctgattcagt
    19141 aaagtacaaa attgctaact tgaaaaataa actatgatta tttaccttaa ttggtgatat
    19201 atttatagca tcaaaattgc aatacggtaa ttctttcttt aaatactcac ccagtgaaaa
    19261 cagaggatag tgttgaggag caagtacaca aatatctttt tcatgaatgc cgctttcaat
    19321 cttttccttt attatatttt ttattatgtt gggtaaatcc tccttagtaa ctttgcaatc
    19381 ataacttata aaaccacggt cgtttttatt attccctctg ctttcaatct cgtaaggaca
    19441 actctgaaaa ttagagtaga agtctactaa tctctgatta gacctgtaac agccatgtag
    19501 cgtctttgat tgaaaagtgt gactagttag catttctagt tcttcaactg atttagctat
    19561 accatctaat gatccataaa ttgcttggtc aatatctcca acaaacattg actgaatttg
    19621 agggttgttt tgaagtaatt tagctattac attgtattgt aattctctag tatcttgaaa
    19681 ttcatccaca taaatcgccc ttatattact agcaatatta cgtgcaacgt tactgtttac
    19741 agttaaaatt ttatacgcta ctagtaaaat catatcaaaa tcaatttctt tatttttttc
    19801 caacatagtg tgatacattt cagaaacttg agtatactga acttcattaa ttttaaaatc
    19861 atgagaaagt gacaaatcaa ttttgttcag ttcatacgtt ttgagattta aattcagcat
    19921 attattaatt tggctgacgt aagctctttg agtataatca tcaataattg taaatccttt
    19981 tgatactctt gagagattca ttttaaaagg ataaataata aattcaagac aaaactgatg
    20041 tattgtcccc acccaaatat taatatcatc atctatacta agtaaactta ggcgttcctt
    20101 aatttcttca gcagctctat tagtgtatgt gatagcaatt attcttttta gtgatccatc
    20161 gtgtttaatt gcttggtaag caattttcct tgtaagtacc cttgttttcc cactcccagg
    20221 acatgcattt acataaaggt tatcttcact ttccacaaca tctttttgtt ctaaatttaa
    20281 atcatttacg attttttcga agatatttgt cattttgaaa cctcagcata aagtattttt
    20341 gtgtaatttt ctatctgatt gattagatac acaacatcct catctttacc aaagtactct
    20401 aataaagaaa ttattgctat attcaaatca gctgtatcac ttgtttttaa actagctgat
    20461 aaagctgaca attctgtagt ttcctcgtac aagtcgattg tatggagtag aattttttta
    20521 taaattgaga gatctactat ttcctgacat gcaaaagcaa tagcattaag aatatactta
    20581 ggaattttga ttaaaaagtc aattttttca cctaactcaa ttgcaaacca ccccttacca
    20641 acatgattgg ctagttttaa catcgctaaa tttctttcta aatattgttc actttgtatt
    20701 tcttctttat aagcagttat tgttttttta tctttgaatg cgtcatctat aattggactg
    20761 acataagtta gaatattttc tttattcaca tctaaaaact caatttcaaa tgttgtatta
    20821 gcataaaaac aattaaccca attgttttca ttaaaaaggg aggataattt ttcttttctt
    20881 gcttgacctt tctcttgtgc agtgctttta tacaatcgat gttctgatgg gattgcctgt
    20941 tcatctaaat cagttacaat agcacacttt ctctgtattc tatcatcgtc aaacaagcta
    21001 gcgatatatt caaacgaagt actaccaaca ttaatcaaac ctattccaag ttcgtctaag
    21061 tttataccaa ataatttttt tactatttga ggaataagta tttcttcagc atctccttca
    21121 actaagataa cacctttaga aaaaagcaat ccatttcttt taacatctaa atatctctct
    21181 actgcgttta ttagatttag attgttcttt tttaactttt ctttagcaaa tgagttcaga
    21241 ttatttgctg gattcataac gatagatttg ccattaaacg attttaatac attcatccga
    21301 gatatttcag acacttcagc taaatgtaca gaatgtgtgg tcatgagaat ctgagtatag
    21361 tttttttgaa gatttaatcc ttcgaacaga gttttttgaa tatgatgatg aatatgagct
    21421 tctggttctt caatcaacat aatattaagt agttctcttg aacgacacgc ttcaaattcg
    21481 actattttca aagctaaata aatcatattt agatgtccta accctagtaa gtctaagtcg
    21541 atattttgtt ctggcttcat tgaaagaaat tttgctaaag aacccatatc atctgacata
    21601 gttgaggaaa gaagaagatt aggagaatac actgaaccaa ttatgctatt tagttgccga
    21661 ttaatatttt cgccaatttt cctaatttca ggaacactgg taatagtctc atttaattgc
    21721 tgtataattg tttttaatga ctcaacatta tctgatgata tttttgattc aattgtttcc
    21781 atgattcttt tgactgggtt tcgattgttt ttcatttctc taagaacatc tcgaagagcg
    21841 tcaacataaa caaccgaaat atgcttgaaa atatctgaca tgtcaattct tgagcccaac
    21901 agagcttcat cgtcttcttc aggattacta gctatattgt cattgagtct tcctaccaat
    21961 ttatcataat tttcattgat acagaaattt agatttgatt tagatgtgta gtaaaattca
    22021 tagtcggata gacgaataga atttctaaat tcgttaaatt gatgagagtc taattcagtg
    22081 gaaatatcaa ataattgctt tctaatagct ttgttaggtc taataaatag agaaatacta
    22141 cctacacctt tatctgcatt tgaaatcaat tcttctaaga ttgtttgact ttcagaattt
    22201 aaagaaatgg aagcacaaat ctcattgtca aattcttctt cgctgattcc ttcaaatgtc
    22261 gcagagataa taatccaatg cccttgccac ccattttgaa ttccatgaaa aaaatctgat
    22321 tcttttagtg ttttactact atagtaataa ctatcatcta ataagattct caatgcagta
    22381 agagcattgg ttttccctga atcattctca cctataatag tatttgtctc attatcgaat
    22441 cgaaagattt cggattcata atttctaaaa tttcgaattt gaagagtaga tataaacata
    22501 agcagatctc ctaactattt aattaatatg attatatagt aagtttatca taaattatgt
    22561 tagtaaaagt tctcattata agggagcgtc aataattttg tgtaaatgat tcttccctac
    22621 tgcagattgt ttctaattct caatcagttg gcttagcatt tgttcgagct cgccttcggc
    22681 ctgttggaaa cctt
//
LOCUS       DACIWG010000171        20862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.045, whole genome shotgun sequence.
ACCESSION   DACIWG010000171 DACIWG010000000
VERSION     DACIWG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.045"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            267..1304
                     /locus_tag="IYF54_002767"
     CDS             267..1304
                     /locus_tag="IYF54_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9833015.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IYF54_002768"
     CDS             complement(1393..1716)
                     /locus_tag="IYF54_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ9833016.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IYF54_002769"
     CDS             complement(1703..2410)
                     /locus_tag="IYF54_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ9833017.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IYF54_002770"
     CDS             2496..2837
                     /locus_tag="IYF54_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ9833018.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IYF54_002771"
     CDS             complement(3008..3856)
                     /locus_tag="IYF54_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833019.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IYF54_002772"
     CDS             4454..5317
                     /locus_tag="IYF54_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9833020.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IYF54_002773"
     CDS             5417..5899
                     /locus_tag="IYF54_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ9833021.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IYF54_002774"
     CDS             complement(6162..6629)
                     /locus_tag="IYF54_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ9833022.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IYF54_002775"
     CDS             6752..7603
                     /locus_tag="IYF54_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9833023.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IYF54_002776"
     CDS             complement(7854..9812)
                     /locus_tag="IYF54_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9833024.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IYF54_002777"
     CDS             complement(9809..10681)
                     /locus_tag="IYF54_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ9833025.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IYF54_002778"
     CDS             complement(11023..11895)
                     /locus_tag="IYF54_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ9833026.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IYF54_002779"
     CDS             12023..12592
                     /locus_tag="IYF54_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ9833027.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IYF54_002780"
     CDS             12659..13396
                     /locus_tag="IYF54_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ9833028.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IYF54_002781"
     CDS             13627..14895
                     /locus_tag="IYF54_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ9833029.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IYF54_002782"
     CDS             15382..15846
                     /locus_tag="IYF54_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ9833030.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IYF54_002783"
     CDS             15830..16459
                     /locus_tag="IYF54_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ9833031.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IYF54_002784"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IYF54_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9833032.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IYF54_002785"
     CDS             18338..18784
                     /locus_tag="IYF54_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ9833033.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IYF54_002786"
     CDS             complement(18983..19498)
                     /locus_tag="IYF54_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ9833034.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IYF54_002787"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IYF54_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6630 a         3703 c         4316 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DACIWG010000172        15975 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.057, whole genome shotgun sequence.
ACCESSION   DACIWG010000172 DACIWG010000000
VERSION     DACIWG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.057"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(10..885)
                     /locus_tag="IYF54_002788"
     CDS             complement(10..885)
                     /locus_tag="IYF54_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ9833035.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1033..1365
                     /locus_tag="IYF54_002789"
     CDS             1033..1365
                     /locus_tag="IYF54_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ9833036.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1477..2685
                     /locus_tag="IYF54_002790"
     CDS             1477..2685
                     /locus_tag="IYF54_002790"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ9833037.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2839..3294
                     /locus_tag="IYF54_002791"
     CDS             2839..3294
                     /locus_tag="IYF54_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833038.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3656..3931
                     /locus_tag="IYF54_002792"
     CDS             3656..3931
                     /locus_tag="IYF54_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ9833039.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3944..6022
                     /locus_tag="IYF54_002793"
     CDS             3944..6022
                     /locus_tag="IYF54_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ9833040.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6182..8068
                     /locus_tag="IYF54_002794"
     CDS             6182..8068
                     /locus_tag="IYF54_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ9833041.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            8080..9027
                     /locus_tag="IYF54_002795"
     CDS             8080..9027
                     /locus_tag="IYF54_002795"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ9833042.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9047..9574
                     /locus_tag="IYF54_002796"
     CDS             9047..9574
                     /locus_tag="IYF54_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ9833043.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9637..10290)
                     /locus_tag="IYF54_002797"
     CDS             complement(9637..10290)
                     /locus_tag="IYF54_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ9833044.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10422..11264
                     /locus_tag="IYF54_002798"
     CDS             10422..11264
                     /locus_tag="IYF54_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ9833045.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11422..12318
                     /locus_tag="IYF54_002799"
     CDS             11422..12318
                     /locus_tag="IYF54_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ9833046.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12321..13034
                     /locus_tag="IYF54_002800"
     CDS             12321..13034
                     /locus_tag="IYF54_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ9833047.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13054..13572
                     /gene="msrA"
                     /locus_tag="IYF54_002801"
     CDS             13054..13572
                     /gene="msrA"
                     /locus_tag="IYF54_002801"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ9833048.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13569..13796
                     /locus_tag="IYF54_002802"
     CDS             13569..13796
                     /locus_tag="IYF54_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ9833049.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13948..14499
                     /gene="lepB"
                     /locus_tag="IYF54_002803"
     CDS             13948..14499
                     /gene="lepB"
                     /locus_tag="IYF54_002803"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ9833050.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14582..15124)
                     /locus_tag="IYF54_002804"
     CDS             complement(14582..15124)
                     /locus_tag="IYF54_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ9833051.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15361..15759)
                     /locus_tag="IYF54_002805"
     CDS             complement(15361..15759)
                     /locus_tag="IYF54_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ9833052.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         5540 a         2747 c         3216 g         4472 t
ORIGIN      
        1 tcttttttct tatagttttt tcagtaatct tgtctgtggc ttcaaataag taaagccttg
       61 cccttccact tcatgcacat tgatgattga aaggaaagct ttttcgtcaa tctcgtggac
      121 gatcctctta gcttcagtta attcactaga gctaacgacc atatagatga ttttttttga
      181 attaccagag aatgcgcctt ctccagaaag gaaagtcaca cctctttgca gaccggtcat
      241 cagtagtggt gcaatctctt cgcttttgtt ggagacgaca agtagacctt ttgcagaata
      301 tccgccatct aagatcgaat caatcacacg actgaaaaca tacgaaacaa tcaacgtata
      361 catcatacgt ttcaagtcga tataggttaa tgaagcaagt aagacgatga tatcaaatgc
      421 caatagggaa cgccccatag tgatgccgta attttgttct aaaatccgag cgataatatc
      481 actgccgcct gttgttccac ccatgcggta gacgatcccg cttcctacac ctgcaactaa
      541 acccgcaagt agagaaacga tcagtagatc atgctgtaaa ttgatttcta gtggaaccct
      601 ttgccagatc cataaaaaga atgacaaaga aagtgtacct agaacagtat aggccaagga
      661 acgtttaccg aggatctttc ctcctagtaa aatcaatggg atgttgatta ataaagtcgt
      721 ataagctgga tcaataccaa ataaagctct taagatcaag gtgatccctg agactccccc
      781 ttctgctaaa tgattagcga tgttcaagta aacaagacca aatgcataaa tacttgttcc
      841 tataatgata aaaagaactt cttttactgt ttttcgtttg accatatgaa ttccagcttt
      901 caattatgta ttttcacaga atttccattt agaaattcct tcctaaatgt agcatggaaa
      961 ttcgattttc tcaattgctc ttatctagga tttgcgttaa agttttggta agatagtttt
     1021 ggagggaaaa agatggataa aaagcgttct ttatacagta tgcagcagga agtggatgaa
     1081 tatatccagc aattcaaggt ggggtatttc tctccattag ctcagatggc tcgattaact
     1141 gaagaagtag gtgaattggc aagagaagtc aaccacactt atggagaaaa aagcaaaaag
     1201 gcttctgaac cagttaactc ggtagcagaa gaacttggtg atgttttatt cgttacgatg
     1261 atcatggcta attcattgaa tatcgactta actgaagttt ttgaaaaaaa catggaaaaa
     1321 ttcaatcaaa gagaccataa tcgctttgag cgtaaggacg agtaaacaag atgaagtggt
     1381 cctggccttg aagactctga aacggacttg tcaaacagtc accatgcaac tgagtcttta
     1441 tctcaggaca agaaacacgc aggaaaggtt ggaaaaatga aattaaaaca attacctctg
     1501 gaataccaaa aggcaattcc agttctgaaa aaattagaga atgcaggata tgaagcttat
     1561 tttgtaggtg gaagtgttcg tgatatcttg ctgaaccagc caatccatga tgtggatatc
     1621 gcaacaagcg catttccaga agaaatcaaa cagctttttc caaaaacgat agatattggg
     1681 atcgaacatg gaactgtgtt ggtattagaa aatgatgaac aatatgagat caccacattt
     1741 cggacggaat caacttatca ggactaccgt agaccagatc atgtcgaatt cgttcgttcg
     1801 ttggcagaag atttgaaaag aagagatttt acgatcaatg cgtttgcttt gaaagaagat
     1861 ggagaaatca tcgatttgtt cgatggtctg caagatctag aagaacagat cttgcgagct
     1921 gtaggcaatc ctcatgagcg gttccatgaa gatgccttac gaatgatgcg cgggttgcgt
     1981 tttgtcagtc aactagggtt caaattagag gaagaaacct tctacgccat caaagaaaac
     2041 cacgagctat taggtaaaat atctgtagaa cgtataaatg tggaattcat caagatgctc
     2101 ttgggaaatg aacgggccgc gggaatccat gcatttattg aaacagagtg ctacatttgc
     2161 tgtcccggtt tgaaagaaga aggcgaagca cttttacgtt ttgctgaatt accatccgtc
     2221 ccattgaaag aagagagcca agcatgggca ttattgatca gccaactgga tctaaaagaa
     2281 aatgagattc gttccttttt gaaatcgtgg aagtgttcta accaaatgat taaagacgtt
     2341 caagcacttg tccaaggact acgacaacga gaaactggag ctttggaacc attcgaattg
     2401 tacttcttag gcaaaaaaca tgcactgcaa gtagaagaat tgatgatcta ttttgacgaa
     2461 acacctcaga tggatgccgt aggagaatcc tatgatcgtt tacctatcaa gtcattaagc
     2521 gaattagcag tagatggtaa aatcttgcta gaagaaactg gaaaaaaacc tggtaagtgg
     2581 gttagtgaag ctttgcaatt agctgaaaaa gccgtcgtag aacaaagaat aaaaaatgat
     2641 aaaaaacagg ttttggattt tttatcgaaa gaaaaattga tttaaaaaac actagactgt
     2701 atctgctcag ctttgttggc tacttaaagt gggcagatac gtcattagaa gaaaaaacat
     2761 aagatgtgac cacttacata aatttgtgct aaactgaggt agtacaagaa taaattacat
     2821 cataaaggag tctttttaat gagtaaagaa atgacagcgc taaaatttta ttttagaaat
     2881 ggagaaacct ggacgatcaa ccgccgtcat atcggcgatc tttggatcaa acaaatcaca
     2941 acaagttttg gacgaatcaa tggcagtgaa tttgttgaaa tccatccgtg tgctggattt
     3001 aaaatcgaaa tcttccatga aggggacgca gttgcgacac atgatatcaa cctaggcgga
     3061 ctggaaatgg gcatgtttaa tcgcgcattg aaatacgaag atatcgagcg aatggaaatc
     3121 ctttaccgaa atggcacacc tgatcttgtg tacttcccat atctagataa aggaacagaa
     3181 ggattagaca atcaatatca atcaacaaaa atcagcgaaa aaacaggtaa cttgtatatc
     3241 gtgatcaatc cagatcaacg tgtagaagat gtatacgggg aatttttcga ataaatcgat
     3301 ttttacgcaa aaatgaggct gtgaaagaga tgtgtcttat tactactgat aggctgtctc
     3361 ttccacaccg tttattcaga ataagggact gtgacaagat ttttgtcgca gccccatttt
     3421 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
     3481 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
     3541 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
     3601 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
     3661 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
     3721 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
     3781 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
     3841 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
     3901 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
     3961 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
     4021 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
     4081 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
     4141 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
     4201 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
     4261 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
     4321 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     4381 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     4441 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     4501 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     4561 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     4621 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     4681 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     4741 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     4801 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     4861 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     4921 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     4981 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     5041 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     5101 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     5161 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     5221 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     5281 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     5341 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     5401 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     5461 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     5521 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     5581 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     5641 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     5701 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     5761 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     5821 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     5881 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     5941 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     6001 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     6061 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     6121 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     6181 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     6241 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     6301 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     6361 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     6421 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     6481 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     6541 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     6601 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     6661 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     6721 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     6781 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     6841 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     6901 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     6961 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     7021 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     7081 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     7141 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     7201 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     7261 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     7321 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     7381 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     7441 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     7501 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     7561 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     7621 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     7681 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     7741 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     7801 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     7861 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     7921 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     7981 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     8041 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     8101 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     8161 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     8221 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     8281 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     8341 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     8401 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     8461 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     8521 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     8581 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     8641 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     8701 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     8761 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     8821 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     8881 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     8941 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     9001 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     9061 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     9121 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     9181 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     9241 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     9301 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     9361 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     9421 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     9481 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     9541 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     9601 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     9661 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     9721 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     9781 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     9841 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     9901 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     9961 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
    10021 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
    10081 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
    10141 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
    10201 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
    10261 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
    10321 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
    10381 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
    10441 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
    10501 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
    10561 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
    10621 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
    10681 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
    10741 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
    10801 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
    10861 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
    10921 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
    10981 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
    11041 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
    11101 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
    11161 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
    11221 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
    11281 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
    11341 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
    11401 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
    11461 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
    11521 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
    11581 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
    11641 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
    11701 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
    11761 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
    11821 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
    11881 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
    11941 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
    12001 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
    12061 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
    12121 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
    12181 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
    12241 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
    12301 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
    12361 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
    12421 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
    12481 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
    12541 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
    12601 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
    12661 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
    12721 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
    12781 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
    12841 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
    12901 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
    12961 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
    13021 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
    13081 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
    13141 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
    13201 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
    13261 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
    13321 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    13381 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    13441 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagagtatta taaaaagcat
    13501 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    13561 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    13621 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    13681 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    13741 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    13801 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    13861 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    13921 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    13981 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    14041 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    14101 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    14161 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    14221 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    14281 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    14341 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    14401 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    14461 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    14521 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    14581 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    14641 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    14701 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    14761 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    14821 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    14881 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    14941 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    15001 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    15061 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    15121 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    15181 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    15241 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    15301 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    15361 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    15421 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    15481 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    15541 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    15601 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    15661 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    15721 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    15781 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    15841 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    15901 cactttcact taactaatag atggaatttc tttataacgc cgattgtaaa attaagctag
    15961 acaactaaaa aagtc
//
LOCUS       DACIWG010000173        14338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.063, whole genome shotgun sequence.
ACCESSION   DACIWG010000173 DACIWG010000000
VERSION     DACIWG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.063"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(101..544)
                     /locus_tag="IYF54_002806"
     CDS             complement(101..544)
                     /locus_tag="IYF54_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ9833053.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="IYF54_002807"
     CDS             complement(739..2079)
                     /locus_tag="IYF54_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9833054.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="IYF54_002808"
     CDS             2361..3257
                     /locus_tag="IYF54_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ9833055.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="IYF54_002809"
     CDS             complement(3400..4341)
                     /locus_tag="IYF54_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ9833056.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="IYF54_002810"
     CDS             4467..5588
                     /locus_tag="IYF54_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ9833057.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="lsa"
                     /locus_tag="IYF54_002811"
     CDS             5846..7348
                     /gene="lsa"
                     /locus_tag="IYF54_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ9833058.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..7942
                     /locus_tag="IYF54_002812"
     CDS             7415..7942
                     /locus_tag="IYF54_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ9833059.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8154..9215
                     /locus_tag="IYF54_002813"
     CDS             8154..9215
                     /locus_tag="IYF54_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ9833060.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9218..10114
                     /gene="murQ"
                     /locus_tag="IYF54_002814"
     CDS             9218..10114
                     /gene="murQ"
                     /locus_tag="IYF54_002814"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ9833061.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10128..12110
                     /locus_tag="IYF54_002815"
     CDS             10128..12110
                     /locus_tag="IYF54_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ9833062.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="IYF54_002816"
     CDS             complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="IYF54_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ9833063.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12837..14261
                     /locus_tag="IYF54_002817"
     CDS             12837..14261
                     /locus_tag="IYF54_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ9833064.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4625 a         2591 c         3033 g         4089 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt
     9061 tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga
     9121 atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta
     9181 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     9241 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca
     9301 agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact
     9361 gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt
     9421 gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt
     9481 accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc
     9541 aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat
     9601 caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     9661 atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc
     9721 ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg
     9781 accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt
     9841 gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt
     9901 cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc
     9961 gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa
    10021 actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc
    10081 tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg
    10141 aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg
    10201 ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg
    10261 taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc
    10321 tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag
    10381 caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg
    10441 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    10501 aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa
    10561 tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact
    10621 tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca
    10681 tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag
    10741 tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga
    10801 tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag
    10861 gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc
    10921 ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca
    10981 tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg
    11041 tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta
    11101 cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac
    11161 aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag
    11221 gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag
    11281 aaatgatcaa aggggcttta ccagtcggaa tcttaggaat cggcgaacca ttgatttacg
    11341 gagtaacatt acctctagga cgtccattta ttactgcttg tatcggtgga ggtatcggtg
    11401 gggcagtgat cggaatgatc ggaaatgtcg gagcaatcgc tattggacct tcaggagccg
    11461 ctttgattcc attgatctct gatggtaaat ggttaggcta tatccttggt ttattggctg
    11521 cttacgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    11581 aagaagaact agcgaccgaa acagccacag taaatgaagt acctgaaaaa gcagtacctc
    11641 aagcttcagc tgaaagtgtg actatcttat catcagttgc tgacggaaca gttgtttctt
    11701 tagataaagc aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt tactttgtag
    11761 aaccagaaaa cggacatatc tattccccgg ttactggagt aataagttct gttttcccaa
    11821 caaaacatgc tatcggcatt acgacaccaa atggcttaga aatcttgctt cacatgggca
    11881 tcaacacagt tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa ggacagcaag
    11941 tgacacctga tacgcttgta gcagaagctg acttagaagc aatcaaagca gcagacaaag
    12001 aaacctccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    12061 aaacaggtaa agcgaaagca aaagcacctg tgatggatgt caaagcttaa taagaataga
    12121 aacaatagag aacataaaaa gaagaagccg aaatttattt ttcagcttct tcttttgtgt
    12181 tatttatttt tttctgccaa aatttgataa acgcgtgtca catcagatgg tgttccccgt
    12241 aattcataat ctgccaagaa aggaaagccg aatctttgtg cgtattgttt ggctgtcaga
    12301 caatattgat tgttgaagtt tttattaccg ctaccgacta caccaaggca aagtttatga
    12361 ttgtcttcgt attccagata ttcgcgcatc gtttcggtca agatttctgt gtcaccgttg
    12421 tctaacccat tcccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
    12481 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
    12541 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaagcacg cgtatttcct
    12601 gaaatcgata tatataaaat gttcatggat catcccccaa ttctttccct ttagtatagc
    12661 atctttttaa gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
    12721 atgagaaaaa agtcggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
    12781 aaaaaaggtt tgttatactt tttgataaaa gcggttttac tttggaggat gcttacatgg
    12841 atacagagca acagaaaaaa ggaattggtt tgattccact tgctgcctta gtgattggtt
    12901 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggtggcc
    12961 cagctatctt cagctgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca
    13021 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
    13081 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
    13141 gaaacgtggc gttcgcaaca ttgttgatga gtgcattagg ttatttcttc cccgtattcg
    13201 aaggtggtca aaatgtggca tcgatcgtga tgagtacgat cttcttatgg ggatatgcat
    13261 ggctagtgaa tcgtggagtt gaaaatgcta gctttgtcaa tgctatcgtc acattttgta
    13321 aattagtgcc attattttta tttattgtag tagctatcag caccttttcg atccaacaat
    13381 tcattaataa cttttctagc gatatcatat tgaatgcgaa tgggcagccg atcccgtttt
    13441 ctagtcaagt attgagttgc atcatggtga tgctctgggt attcgttggt attgaaggag
    13501 cttctgtcgt aggttcacga gcaaaaaaac aatcggatgt tgggaaagca acgattctag
    13561 gaatattcgg tttgatcatt atttacgtct tggtttccat gcttccatat ggcacaatga
    13621 cgatgaatca gctgcaaacg atcaaagcgc aacctgctat gggatatgta tttgaaatga
    13681 tggttggtaa atggggggcg gtcgtgatca acggcggttt gatcatttct ttagttgggg
    13741 tttggctctc atggacaatc ttgccgattg aaacaatggg gaatatggct gaggacggac
    13801 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
    13861 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcatata
    13921 actttgctta tacacttggt acagcagcga ttttcttcac gtggcttttc ttagggctgt
    13981 accaaatgaa gctttcttat cagcggaaag agtgggtcca atttttagta gggtttttag
    14041 caagtgcttt tcaagtatgg gccatgctag ttgtagcctt taatgaagtg atgcttgttc
    14101 ttgttctttt cttaccaggt atttattttt acctcaaagc acgtaaagaa caaaacgaac
    14161 ctgtacaaca tatccactta gataagattt tacttatcgg tgtgacgatg gtcggagtaa
    14221 tcgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa gagaggctgt
    14281 gaagcctgtg gtttattcaa aaaaggctct ttgtcaaatg atgttggtaa agagaaat
//
LOCUS       DACIWG010000174         7780 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.088, whole genome shotgun sequence.
ACCESSION   DACIWG010000174 DACIWG010000000
VERSION     DACIWG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.088"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            245..1213
                     /locus_tag="IYF54_002818"
     CDS             245..1213
                     /locus_tag="IYF54_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ9833065.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IYF54_002819"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IYF54_002819"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ9833066.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IYF54_002820"
     CDS             2243..2851
                     /locus_tag="IYF54_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ9833067.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /gene="vanY"
                     /locus_tag="IYF54_002821"
     CDS             3279..4190
                     /gene="vanY"
                     /locus_tag="IYF54_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAQ9833068.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERVPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IYF54_002822"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IYF54_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ9833069.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IYF54_002823"
     CDS             complement(5115..5444)
                     /locus_tag="IYF54_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9833070.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="IYF54_002824"
     CDS             5749..6078
                     /locus_tag="IYF54_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ9833071.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="IYF54_002825"
     CDS             6068..6355
                     /locus_tag="IYF54_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ9833072.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="IYF54_002826"
     CDS             complement(6634..7197)
                     /locus_tag="IYF54_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9833073.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1297 c         1546 g         2370 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagt ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctaa
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DACIWG010000175         6031 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.099, whole genome shotgun sequence.
ACCESSION   DACIWG010000175 DACIWG010000000
VERSION     DACIWG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.099"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            199..864
                     /locus_tag="IYF54_002827"
                     /pseudo
     CDS             199..864
                     /locus_tag="IYF54_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            900..1145
                     /locus_tag="IYF54_002828"
     CDS             900..1145
                     /locus_tag="IYF54_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ9833074.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1248..2117
                     /locus_tag="IYF54_002829"
     CDS             1248..2117
                     /locus_tag="IYF54_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ9833075.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            2098..2832
                     /locus_tag="IYF54_002830"
     CDS             2098..2832
                     /locus_tag="IYF54_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ9833076.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2865..3773
                     /locus_tag="IYF54_002831"
     CDS             2865..3773
                     /locus_tag="IYF54_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ9833077.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3770..4312
                     /gene="sat4"
                     /locus_tag="IYF54_002832"
     CDS             3770..4312
                     /gene="sat4"
                     /locus_tag="IYF54_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ9833078.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            4405..5199
                     /locus_tag="IYF54_002833"
                     /note="aph(3')-IIIa"
     CDS             4405..5199
                     /locus_tag="IYF54_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ9833079.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5307..5495)
                     /locus_tag="IYF54_002834"
                     /pseudo
     CDS             complement(5307..5495)
                     /locus_tag="IYF54_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5475..6017
                     /locus_tag="IYF54_002835"
     CDS             5475..6017
                     /locus_tag="IYF54_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ9833080.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2071 a          900 c         1322 g         1738 t
ORIGIN      
        1 aagggatata ttgattttat ctctttttca aaataaacat ggggctgacc caaaagtagc
       61 tcctatatga aaaatagagg tctttccatt tagatagtag tggaaagacc tctatttatc
      121 ttttgaaagt gaatacaaaa ataaagcaca ctctgctata atataggtga ttaatccatt
      181 tgagagaggt gctttatcat gtatcaaaat tataccataa accaactttg tttgcctata
      241 gatcttgaaa ttaaattaga agaaaatgat tttgctcatg ccattgtgca gtttgttgac
      301 tctattcccg atgaagtctt tcttccttat tatcaatcta tgggtagacc ccaatatcat
      361 cctcgtatgc tactttccat tattctttgt gcctatattc aaggggttta ttcgggccgc
      421 caaatacaaa atatgctgat agatagtatt cggatgcgct acttatctca agagcaattc
      481 cctaactttc gtactattaa tcgttttcgc gttcatccga ttatgaacga tattctagat
      541 cattcatttg ttcaatttcg tgagctactg gttcaaagtg gcttaatcag tggcagtgct
      601 ctatacattg atggtacaaa aattgaagcg gatgcgaata aatactcttt cgtttggaaa
      661 aaaagtattt taaagtataa ggatagcctt gatcaaaaag cccttgaaaa ctaccaacaa
      721 atggtggaaa caaaaatctt acctgagtta atagatgatt taaaggatga actttctata
      781 gaagagattg aaaggattcg tcaatctttg gaagaaaagg aaaatcaatt aattgaagcg
      841 attgaccaaa ccgaaacagt agaagggggc tgacccatta ttaataagga gtggacaaca
      901 tgattgatgt aaatgagcta ttggaagaag ctattagaga aactgaaaat ctaaatgatg
      961 gcgaagtttt tcttgttaaa gatttattca agggatatgt ggggaatagg atacccagaa
     1021 aggatcgact tctgcttgga acgttatttt taaaccatgt tagcaaaatg aatggtaatt
     1081 taaaggctat tgaaaagact tcatctaatc agcagaggta taaaaagaca attgataagt
     1141 tttaactatc ttttgtgaaa ttaaaaaaat tataaatatg ttgagtagaa gcaaaagatt
     1201 tggcttggaa tttttacagc agtgaatcga taaaaggagc atgcttgatg gtagataata
     1261 ttattaaatc agtagcagag aaattatcct ctctgtctta tatagaaggt attgttttag
     1321 gtggttcacg tgcaaggggc acccatacag aggattcgga tatagatatc ggcatctatt
     1381 acaattcaga atcatttgac ataaatacta ttaatcaatt cgctacaaag ctggatgatg
     1441 agcatagaaa taaccttgtt gtacctcccg gagcatgggg tgattggatt aatggcggcg
     1501 gatggttagt cataaacggg tatcatgtgg atttaatttt acgtgatatt aaacgtgtgg
     1561 aacaaataat gaaagataca gagcacggaa ttgttactgc caattatcag actgggcatc
     1621 cccatggtta tattagtgca atgtatcgag gagaattagc gattagcaaa atactatatg
     1681 ctaagaatga aagcttatgc gaattaaaaa aacaggcaga aacttatccc aatgctttgc
     1741 agaaaagttt agttaacttt tttatgtttg aagcagggtt ctctttaatg tttgtaaaag
     1801 caaattcggg aacagacgat aaatattata ttgcgggtca tgtttttcgt atagtttcat
     1861 gtttaaatca agtgttattt gcatgtaata atgcttattg tatcaacgaa aagaaagcta
     1921 taaaactgct tgaaactttt gaacataaac ctgaaaaata taccgagaag gtaaatcata
     1981 tttttgaagt actcggtatc tcactttttg aatgctacga catgaccgag aagctttata
     2041 atgaagtgaa tgaaattgta tcggagataa ataacttttt aaacgaggag agttcagatg
     2101 aaagaaaaca aatatgatga taatatattt tttcaaaaat acagtcaaat gagtcgctcg
     2161 cagaaaggac tggctggtgc gggagaatgg gagactttga aaaagatgct acctgatttt
     2221 aagggtaagc gtgtgcttga tttaggatgc ggctatggat ggcactgtat atatgcgatg
     2281 gaaaacggtg cttcctctgt agtaggtgtt gatatttctc ataaaatgct cgaagtagca
     2341 aaaggaaaaa cccattttcc acagattgaa tatgaatgct gtgccataga agatgtggat
     2401 ttcccagagg agagctttga tgtaatacta agttcgcttg cgtttcatta tgtagcagac
     2461 tatgagaatt taataaaaaa gatatatagg atgctgaagg ctggtggcaa tttagttttt
     2521 acagttgaac atcctgtttt tactgctcat ggaacacaag actggtatta taacgaaaaa
     2581 ggagaaatac tgcatttccc ggtggacaat tattattatg agggcaaacg gacagctatg
     2641 tttttggaag aaaaggttac aaaatatcat agaacactga ccacatatct aaatacactg
     2701 ctttcaaata gttttataat aaatcagatt gtggagccac agccgccaga gaacatgatg
     2761 gatattccgg ggatggcgga tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa
     2821 aagaagatgt aataatatag aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag
     2881 aaatgatgga tttagtactt tctttagcag aacaggatga acgtattcga attgtgaccc
     2941 ttgaggggtc acgcgcaaat attaatatac ctaaagatga atttcaggat tatgatatta
     3001 catattttgt aagtgatata gaaccgttta tatctaatga tgactggctt aatcaatttg
     3061 ggaatataat aatgatgcaa aagccggagg atatggaatt attcccacct gaagaaaagg
     3121 gattttccta tcttatgcta tttgatgatt acaataaaat tgatcttacc ttattgccct
     3181 tggaagagtt agataattac ctaaagggcg ataaattaat aaaggttcta attgataaag
     3241 attgtagaat taaaagggac atagttccga ctgatataga ttatcatgta agaaagccaa
     3301 gcgcaaggga gtatgatgat tgctgcaatg aattttggaa tgtaacacct tatgttatta
     3361 aaggattgtg ccgtaaggaa attttatttg ctattgatca ttttaatcag attgttcgcc
     3421 atgagctgct gagaatgata tcatggaagg tcggcatcga aacaggcttt aaattaagtg
     3481 taggcaagaa ctataagttt attgaaaggt atatatccga ggatttgtgg gagaaacttt
     3541 tgtccaccta ccggatggat tcctatgaaa acatatggga agcattattt ctatgccatc
     3601 aattgttcag ggcggtatcc ggtgaggtgg cggaaaggct tcattatgcc tatccggagt
     3661 atgataggaa tataacaaaa tataccaggg acatgtataa aaaatacact ggtaaaaccg
     3721 gctgcctgga tagcacatat gccgctgata tagaagagag gcgggaacag tgattacaga
     3781 aatgaaagca gggcacctga aagatatcga taaacccagc gaaccatttg aggtgatagg
     3841 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
     3901 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
     3961 tgaatatatt gacaatactg ataagataat atatctttac taccaagacg ataaatgcgt
     4021 cggaaaagtt aaactgcgaa aaaattggaa ccggtacgct tatatagaag atatcgccgt
     4081 atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg
     4141 ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc
     4201 ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta tgttatacgc
     4261 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
     4321 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     4381 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     4441 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     4501 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     4561 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     4621 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     4681 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     4741 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     4801 acgaatagct tagacagccg attagccgaa ttggattact tactgaataa cgatctggcc
     4861 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     4921 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     4981 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     5041 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     5101 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     5161 aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt agatgtctaa
     5221 aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat ccgcaactgt
     5281 ccatactctg atgttttata tcttttctaa aagttcgcta gatagagttc tatattagag
     5341 atgtaaagag ttttggtgaa gaggtttgtg agtatggctt ttataaccag ggtgcaatga
     5401 gaagcactaa cagcagctag cttaagaacg cataggatat actttttatg gaagtcacag
     5461 aggaggaata aaatttgaac aggattaaca gagtaacctc tattcttatt cagctgcaat
     5521 caaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc ttaaggacag
     5581 tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct gaggccggaa
     5641 aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca gcggcggaag
     5701 tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa tcatttataa
     5761 aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt gaaaaaaact
     5821 atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag aaaaatacat
     5881 tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg gtaatatcaa
     5941 ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag ccggttctgt
     6001 tgcaaagttt taaataaaga ataaaatccc t
//
LOCUS       DACIWG010000176         4944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.109, whole genome shotgun sequence.
ACCESSION   DACIWG010000176 DACIWG010000000
VERSION     DACIWG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.109"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            21..1061
                     /locus_tag="IYF54_002836"
     CDS             21..1061
                     /locus_tag="IYF54_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ9833081.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1159..1347
                     /locus_tag="IYF54_002837"
     CDS             1159..1347
                     /locus_tag="IYF54_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9833082.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1405..1758)
                     /locus_tag="IYF54_002838"
     CDS             complement(1405..1758)
                     /locus_tag="IYF54_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9833083.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2289..2711
                     /locus_tag="IYF54_002839"
     CDS             2289..2711
                     /locus_tag="IYF54_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ9833084.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2708..2938
                     /locus_tag="IYF54_002840"
     CDS             2708..2938
                     /locus_tag="IYF54_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ9833085.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3436..3636
                     /locus_tag="IYF54_002841"
     CDS             3436..3636
                     /locus_tag="IYF54_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ9833086.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3663..4856
                     /locus_tag="IYF54_002842"
     CDS             3663..4856
                     /locus_tag="IYF54_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ9833087.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1705 a          798 c          912 g         1529 t
ORIGIN      
        1 aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata
       61 gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc
      121 ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac
      181 aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg
      241 aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta
      301 tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt
      361 atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa
      421 atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt
      481 tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg
      541 gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac
      601 aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact
      661 atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag
      721 cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac
      781 cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa
      841 atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc
      901 aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc
      961 taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc
     1021 ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata
     1081 taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt
     1141 ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat
     1201 aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc
     1261 aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag
     1321 ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct
     1381 ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt
     1441 tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac
     1501 ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga
     1561 tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt
     1621 ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt
     1681 gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt
     1741 atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat
     1801 aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt
     1861 tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa
     1921 ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga
     1981 aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa
     2041 aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca gattacatag
     2101 atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac
     2161 ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg
     2221 ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg
     2281 tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct
     2341 gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag
     2401 caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt tctactgttg
     2461 atacgtacgt cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa
     2521 gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac
     2581 ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt
     2641 cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt
     2701 gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga
     2761 tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg
     2821 ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct
     2881 acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc
     2941 ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt
     3001 attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc
     3061 tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa
     3121 cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta
     3181 gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc
     3241 gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc agaagtgaaa
     3301 ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt gtcagtgtat
     3361 tattataaaa ctatttgata taattatagg tgaatattgg agttttccta gcctataatt
     3421 gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct aggatatgga
     3481 acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac aaaagggcat
     3541 atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga agaaattcta
     3601 ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa
     3661 ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc
     3721 taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc actactaaaa
     3781 aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa ttaagtgaga
     3841 ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca tggtataaga
     3901 cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag catttctctt
     3961 attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat tggcaactaa
     4021 aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta ttttcagttg
     4081 ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag attattggca
     4141 atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt gaaaaagtaa
     4201 tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct ctgtggtttt
     4261 tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa gatattgatt
     4321 ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa tcgaattata
     4381 gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat aaatgcacat
     4441 taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca gactttgtaa
     4501 tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt gaacgctatg
     4561 ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct catgcttcct
     4621 tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga cacgaagata
     4681 ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc gaggtagcta
     4741 acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat acttcaccaa
     4801 agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt
     4861 gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttcg gctctataat
     4921 ttatgggact gatgaatcag aatt
//
LOCUS       DACIWG010000177         3720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.116, whole genome shotgun sequence.
ACCESSION   DACIWG010000177 DACIWG010000000
VERSION     DACIWG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.116"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            554..640
                     /locus_tag="IYF54_002843"
                     /pseudo
     CDS             554..640
                     /locus_tag="IYF54_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
     gene            641..2581
                     /gene="tet(S)"
                     /locus_tag="IYF54_002844"
     CDS             641..2581
                     /gene="tet(S)"
                     /locus_tag="IYF54_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ9833088.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2669..2836
                     /locus_tag="IYF54_002845"
     CDS             2669..2836
                     /locus_tag="IYF54_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ9833089.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3350..3706)
                     /locus_tag="IYF54_002846"
     CDS             complement(3350..3706)
                     /locus_tag="IYF54_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ9833090.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1327 a          577 c          618 g         1198 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaacccttt taatgatatt ttccaagtca
       61 aacttaatat taacaattca aaactattgg aaccactgca aaatattttg ttaagcagta
      121 tatatttcaa aaattatacg taatatattt gtagaaaggt tgtggacagt tgtcctattg
      181 acctgaaaag taatataata gctggatgaa taaagaatcc cttatgaact tgtaaatcgt
      241 tattaaatcg ctgaattttg atagccattc tgaagagtta tcttcaacta ctacactaac
      301 ttccattaat ttctcattaa tttccctttc gttcaaaaaa tatggttggc tttacaagca
      361 ttctatcata tgatagaatt aagataacat tcaaaaggag ataataaaaa tgcggcaagg
      421 tattcttaaa taaactgtca attagatagc gggaataatt aattaagcat ccttatttaa
      481 aataaggact ggttttttgt gcccagttta agaatacttt tatcatttta tcataaagca
      541 tctgtggcta tcagtgtgta gtatacagct atttttgtat taaaatccca gtgataagag
      601 tatttatcac tgggattttt tctgcttttt gagcttttga atggaggaaa taaaattgaa
      661 aattattaat atcggtatct tagcacatgt tgatgcagga aaaactactt tgacagaaag
      721 cttactatac agtagcggag caattaaaga gttaggaagt gtagatagcg gtacaacgaa
      781 aacggatact atgtttttgg aacgccagag aggtattact attcagaccg caataacatc
      841 ttttcaacgg gaaaatgtta aagtaaatat tgtagatact cctggacaca tggatttttt
      901 ggcagatgta taccgttcat tatctgtttt ggatggagct attttgctaa tctctgcaaa
      961 agatggagta cagtcacaaa ctcgtatact attccatgca cttagaaaga tgaacatacc
     1021 tataatattt tttattaaca aaattgatca aaatggaata aatttgccag atgtttatca
     1081 agatattaag gacaaacttt ctgacgacat cataattaag cagactgtga atctaaattt
     1141 gaaaccttat gtaatagatt atactgaacc agaacaatgg gagacagtaa ttgtgggaaa
     1201 tgattattta ttagaaaaat ataccattgg gaaaacattg aatattgcag aacttgaaaa
     1261 ggaggaaaac gaaagaattc aaagttgctc cttatatcct gtttatcacg gaagtgcaaa
     1321 gaataatatt ggaattaaac aacttataga ggtaattact agcaaattat tttcacccac
     1381 acaactcaat tcagataaac tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg
     1441 tcaacggctt gtctatgtac gtctttatag tggaacgcta catttgcgag actcagtcaa
     1501 tatatcagaa aaggaaaaaa taaaagttac agaaatgtat acttcaataa atggagaatt
     1561 acgccagata gataaggcag agcctggtga gattattatt ttaaaaaatg agcttttaaa
     1621 actaaataac gtacttggag ataaaaaaag attaccacat agagaaattc ttgagaatcc
     1681 tcttcctatg ttacaaacaa caattgaacc atgtaaatca gtacaaagag aaaagttact
     1741 agatgcactt tttgaaatat ccgatagtga tccccttcta caatattatg tagatacagt
     1801 aactcacgaa attgtgctat cttttttagg tgaggtccaa atggaggtaa cttgtactct
     1861 gattcaagaa aaatatcata ttgagataga aacaagaaaa ccaactgtca tttatatgga
     1921 aagaccatta aaaaaatctg aatttaccat tgatatcgaa gtacctccaa atcctttctg
     1981 ggcttctatt ggtttatctg taacaccact tcctttgggt agtggcattc agtatgagag
     2041 cctggtttct ctaggttatt taaatcaatc atttcaaaat gcagttatgg aaggtatacg
     2101 ctatgggtgt gaacaaggat tgtacggttg gaaattaaca gactgtaaga tctgttttaa
     2161 gtatggtcta tattacagcc ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat
     2221 tgtactagag caggctttta gaaagagtgg tacagagtta ttagagccat atcttagctt
     2281 cgaaatttat gtaccacaag aatatctttc gagagcatat aatgatgctt ccaaatattg
     2341 tgcaaatatt ttaaatacta agttaaaagg taacgaggtc attctcattg gtgaaattcc
     2401 agcccgttgt attcaagagt atcgaaacag tttaactttc tttacaaatg gacgcagtgt
     2461 ctgtttaaca gagttaaaag gttatcaggt tactaacatt aagtctgctt tccaaccacg
     2521 tcgtccaaat aatagaatag acaaagtaag gcatatgttt aataaaatca acttacattg
     2581 atagtgaact aaagagaaat ttttaaaatg aatattgagt tgttaattag aacaggagga
     2641 tatagatatt gtaaatacta ggtggatact gtgccctata tgtggcaata agacccgttt
     2701 aaaaatgagg gaagatactg aattaaaaaa tttccctcta ttttgtccta aatgcagaca
     2761 agaaatttta attgaaataa cgaagtttag aataactgta attacagagc cagacgcaaa
     2821 gacgcagagc cgataattcg agatatcaaa atctcatttt atcggctttt tcttttctat
     2881 gcataccatt gagcttagtt cttgatatcc tttataagaa aataagtgta ctgatttttt
     2941 ttgcagtagc tgtattacag tagacatccc tcaagcataa aaagtataca attaaaattc
     3001 aatacttgat tattgatgaa gtacagagtt actcgtcatt tcaaaactag tatatcagtc
     3061 aagtttttcc taaggaacaa aaacgaactt cgctatggga atcaaaaatt tttagtcgga
     3121 tgaagtgata tgcaataaat ccagaaaatt ttcccaacaa caaaaaatga actgtacttt
     3181 gcagcgataa gagtaaccaa agaactattt atcattcgct ctagttccta agtgtaacaa
     3241 agataaattc attttctatt tatgattaac aaaaatcaga aaaaattcat tacatctttg
     3301 taatcaatct ttcctgatag tattaattaa tccattgtgt attattgaac tagttaaatt
     3361 tacatttttc tccgtaaatt gcttgaatca ctttcccaat cgtcactaat tcatcttcat
     3421 ccagacaatc caactgtgct tctacttgaa gacgtttgga gcttttatga ctgttttctt
     3481 cccctaaaat aaatccatcc aaagaaatat ttagcaaagt cactaaatca tatacaacat
     3541 gaaaacttgg ctgttgtcct ttattttcaa tgttcgtcaa atagcgtggc gcaatctgga
     3601 tcattgatcc tacctgctca cgagtcaatc ctttactaag acgggcttct ttaatcgcaa
     3661 gacccaatgg tttgaaatca aaatttgata gagtagtttg ccatattctt gcacccaacg
//
LOCUS       DACIWG010000178         2551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.126, whole genome shotgun sequence.
ACCESSION   DACIWG010000178 DACIWG010000000
VERSION     DACIWG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.126"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            <1..459
                     /locus_tag="IYF54_002847"
     CDS             <1..459
                     /locus_tag="IYF54_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ9833091.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IYF54_002848"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IYF54_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ9833092.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IYF54_002849"
     CDS             1346..2500
                     /locus_tag="IYF54_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ9833093.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          453 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa t
//
LOCUS       DACIWG010000179         2234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.135, whole genome shotgun sequence.
ACCESSION   DACIWG010000179 DACIWG010000000
VERSION     DACIWG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.135"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            complement(<1..1068)
                     /locus_tag="IYF54_002850"
     CDS             complement(<1..1068)
                     /locus_tag="IYF54_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ9833094.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHAT"
     gene            1621..2169
                     /gene="aac(6')"
                     /locus_tag="IYF54_002851"
     CDS             1621..2169
                     /gene="aac(6')"
                     /locus_tag="IYF54_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ9833095.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          717 a          395 c          437 g          685 t
ORIGIN      
        1 tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc
       61 ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc
      121 ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa
      181 cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc
      241 ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta
      301 atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc
      361 ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc
      421 actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat
      481 ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc
      541 gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc
      601 aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc
      661 cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc
      721 aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc
      781 gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg
      841 gatcaactgt tcaatatttc cataagttga attacccccg taccagagac tggcgacaac
      901 ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt
      961 ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata
     1021 cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt
     1081 ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga
     1141 tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga
     1201 aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg
     1261 aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa
     1321 attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt
     1381 ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt
     1441 gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt
     1501 ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag atttttgagc
     1561 gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt
     1621 atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta
     1681 ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg
     1741 atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt
     1801 ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc
     1861 tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct
     1921 tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta
     1981 agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt
     2041 gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat
     2101 gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat
     2161 tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt
     2221 tacgaaaatt actt
//
LOCUS       DACIWG010000180         1994 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.137, whole genome shotgun sequence.
ACCESSION   DACIWG010000180 DACIWG010000000
VERSION     DACIWG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.137"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            45..437
                     /locus_tag="IYF54_002852"
     CDS             45..437
                     /locus_tag="IYF54_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ9833096.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            438..1877
                     /locus_tag="IYF54_002853"
     CDS             438..1877
                     /locus_tag="IYF54_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ9833097.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          830 a          153 c          342 g          669 t
ORIGIN      
        1 cattgtatag cattttacac aggagtctgg acttgactga gtttatggaa gaagttttaa
       61 ttgatgataa tatggttttt gatattgata atttaaaagg atttcttaat gataccagtt
      121 catttgggtt tatagctaaa gaaaataata aaattatagg atttgcatat tgctatacac
      181 ttttaagacc tgatggaaaa acaatgtttt atttacactc aataggaatg ttacctaact
      241 atcaagacaa aggttatggt tcaaaattat tatcttttat taaggaatat tctaaagaga
      301 ttggttgttc tgaaatgttt ttaataactg ataaaggtaa tcctagagct tgccatgtat
      361 atgaaaaatt aggtggtaaa aatgattata aagatgaaat agtatatgta tatgattatg
      421 aaaaaggtga taaataaatg aatatagttg aaaatgaaat atgtataaga actttaatag
      481 atgatgattt tcctttgatg ttaaaatggt taactgatga aagagtatta gaattttatg
      541 atggtagaga taaaaaatat acattagaat cattaaaaaa acattataca gagccttggg
      601 aagatgaagt ttttagagta attattgaat ataacaatgt tcctattgga tatggacaaa
      661 tatataaaat gtatgatgag ttatatactg attatcatta tccaaaaact gatgagatag
      721 tctatggtat ggatcaattt ataggagagc caaattattg gagtaaagga attggtacaa
      781 gatatattaa attgattttt gaatttttga aaaaagaaag aaatgctaat gcagttattt
      841 tagaccctca taaaaataat ccaagagcaa taagggcata ccaaaaatct ggttttagaa
      901 ttattgaaga tttgccagaa catgaattac acgagggcaa aaaagaagat tgttatttaa
      961 tggaatatag atatgatgat aatgccacaa atgttaaggc aatgaaatat ttaattgagc
     1021 attactttga taatttcaaa gtagatagta ttgaaataat cggtagtggt tatgatagtg
     1081 tggcatattt agttaataat gaatacattt ttaaaacaaa atttagtact aataagaaaa
     1141 aaggttatgc aaaagaaaaa gcaatatata attttttaaa tacaaattta gaaactaatg
     1201 taaaaattcc taatattgaa tattcgtata ttagtgatga attatctata ctaggttata
     1261 aagaaattaa aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac
     1321 aaaatttgtt aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata
     1381 cagatattag tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt
     1441 tgcgtgaaac tatttataat gatttaactg atatagaaaa agattatata gaaagtttta
     1501 tggaaagact aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt
     1561 ttagttgtaa tcatctattg ttagatggca ataatagatt aactggaata attgattttg
     1621 gagattctgg aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag
     1681 aagaaatagg aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg
     1741 agaaagcaaa agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt
     1801 atggaattaa aaatattaaa caggaattta tcgaaaatgg tagaaaagaa atttataaaa
     1861 ggacttataa agattgatta tataatatat gaaaagctat tataaaagac attagtatta
     1921 aatagtttaa aaaaatgaaa aataataaag gaagtgagtc aagtccagac tcctgtgtaa
     1981 aatgctatac aatg
//
LOCUS       DACIWG010000181          772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.173, whole genome shotgun sequence.
ACCESSION   DACIWG010000181 DACIWG010000000
VERSION     DACIWG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.173"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002854"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9833098.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          287 a          137 c          120 g          228 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DACIWG010000182          772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.174, whole genome shotgun sequence.
ACCESSION   DACIWG010000182 DACIWG010000000
VERSION     DACIWG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.174"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002855"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9833099.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          286 a          137 c          121 g          228 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DACIWG010000183          772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V441
            SAMEA5368195-rid9535733.guided.175, whole genome shotgun sequence.
ACCESSION   DACIWG010000183 DACIWG010000000
VERSION     DACIWG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368195
            Sequence Read Archive: ERR3171372
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699855.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699855.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 17:56:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,726
            CDSs (with protein)               :: 2,726
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 62
            CDSs (without protein)            :: 62
            Pseudo Genes (ambiguous residues) :: 0 of 62
            Pseudo Genes (frameshifted)       :: 31 of 62
            Pseudo Genes (incomplete)         :: 28 of 62
            Pseudo Genes (internal stop)      :: 15 of 62
            Pseudo Genes (multiple problems)  :: 10 of 62
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368195-rid9535733.guided.175"
                     /isolate="V441"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2014"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002856"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="IYF54_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ9833100.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNDLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          285 a          136 c          122 g          229 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      241 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagccg tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac gatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
